Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 724,813 A→T 91.4% intergenic (+17/‑226) ybfA → / → rhsC DUF2517 family protein/Rhs family putative polymorphic toxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273724,8130AT91.4% 491.7 / 25.2 163intergenic (+17/‑226)ybfA/rhsCDUF2517 family protein/Rhs family putative polymorphic toxin
Reads supporting (aligned to +/- strand):  ref base A (4/10);  new base T (51/96);  total (56/107)
Fisher's exact test for biased strand distribution p-value = 7.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 163 total aligned reads displayed.

GCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATAT  >  CP009273/724667‑724959
                                                                                                                                                  |                                                                                                                                                  
gcTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCAt                                                                                                                                                 <  2:1081944/150‑1 (MQ=255)
gcTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCAt                                                                                                                                                 <  1:2226327/150‑1 (MQ=255)
gcTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCAt                                                                                                                                                 >  2:2421657/1‑150 (MQ=255)
   gCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGa                                                                                                                                              <  1:4500756/150‑1 (MQ=255)
   gCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGa                                                                                                                                              <  1:4500691/150‑1 (MQ=255)
    ccTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGaa                                                                                                                                             >  2:1066203/1‑150 (MQ=255)
      tttCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGaaaa                                                                                                                                           <  2:1136363/150‑1 (MQ=255)
      tttCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGaaaa                                                                                                                                           >  1:895891/1‑150 (MQ=255)
         cATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCg                                                                                                                                        <  2:4605245/150‑1 (MQ=255)
            gCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCa                                                                                                                                     <  1:1238886/150‑1 (MQ=255)
             cTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAg                                                                                                                                    <  2:1074365/150‑1 (MQ=255)
                  cTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAATCCGCCCGCGGaa                                                                                                                               >  1:2891098/1‑150 (MQ=255)
                  cTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGaa                                                                                                                               <  2:2947958/150‑1 (MQ=255)
                  cTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGaa                                                                                                                               <  2:2947956/150‑1 (MQ=255)
                    ggAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAAc                                                                                                                             <  1:3688933/150‑1 (MQ=255)
                    ggAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAAc                                                                                                                             <  2:3414087/150‑1 (MQ=255)
                      aaaGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGc                                                                                                                           >  1:3206111/1‑150 (MQ=255)
                        aGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTg                                                                                                                         <  1:4317794/150‑1 (MQ=255)
                          aCCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGc                                                                                                                       <  2:1768799/150‑1 (MQ=255)
                             gcgcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGCAAAGGCAACTGGTGATTTTTATTGATTGCTGAGGTTTGAATGCATTGAAAACCGCCAGCGGAACCGCTGGCggg                                                                                                                    >  2:1025251/1‑149 (MQ=255)
                               gcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGttt                                                                                                                  <  1:3472225/150‑1 (MQ=255)
                               gcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGttt                                                                                                                  <  1:3472250/150‑1 (MQ=255)
                               gcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGttt                                                                                                                  <  2:546004/150‑1 (MQ=255)
                                  cGCTTTTACAGCTACCTGCATCGCGTCGGGTCGAAAACCCGCTATAAACGGGTGTGTTTGTATCAGGCCGAAAAGGCAACTGTTTATTTTTATTTATTGCTTAGTTTTTAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGtttttt                                                                                                               <  1:945818/150‑1 (MQ=255)
                                   gCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTg                                                                                                              >  2:3519880/1‑150 (MQ=255)
                                   gCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTg                                                                                                              >  2:988300/1‑150 (MQ=255)
                                      ttaaCAGCTACCTGCATCGGGTCTGGTCTAAAACCAGCTATAAACGGGTGTGTATGGATCCGGCCGAAAAGGCAAGTGTTTATTTTTTTTGTTTGCTGATTTTTGATTTCATTTAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTAt                                                                                                           <  1:4142604/147‑1 (MQ=255)
                                          cAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAgg                                                                                                       >  1:25627/1‑150 (MQ=255)
                                             cTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGTATGGATCAGGCCGAAAAGGCAACTGGTTATTTTTATTGATTGCTGAGTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAg                                                                                                    <  1:250804/150‑1 (MQ=255)
                                             cTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAg                                                                                                    <  2:175002/150‑1 (MQ=255)
                                               aCCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGAt                                                                                                  <  2:4503269/150‑1 (MQ=255)
                                               aCCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATAAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATGGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGAt                                                                                                  <  2:566029/150‑1 (MQ=255)
                                                ccTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCAGTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATg                                                                                                 <  2:2986273/150‑1 (MQ=255)
                                                  tGCATCGCGTCTGGTCCAAAACCAGCGATAAACCGGTGTGGTTGGATCAGGCCGAAAAGGAAACTGGTGATTTTTATTGATTGCTGAGTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTATTTTGTATCAGGAAGATGtt                                                                                               <  1:1040351/150‑1 (MQ=255)
                                                   gCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTc                                                                                              <  1:4721740/150‑1 (MQ=255)
                                                   gCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTc                                                                                              <  1:4721087/150‑1 (MQ=255)
                                                          gTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGCAGATGTTAAGAGGaa                                                                                       >  1:96725/1‑150 (MQ=255)
                                                            cTGGTCGAAAACCAGCTATAAACGGGTGTGGTTGGATCCGGCCGAAACGGCACGTGGTTTTTTTTTTTGTTTGCTGTGTTTTGAATTCATTGAAAACCGCCCGCGGAAACGGTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAata                                                                                     <  1:2381037/150‑1 (MQ=255)
                                                             tGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATAATGAAGATGTTAAGAGGAatat                                                                                    >  1:4568397/1‑150 (MQ=255)
                                                             tGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAatat                                                                                    >  2:4403589/1‑150 (MQ=255)
                                                              gttccaaaaCCAGCTATAAACCGGTGTGTATGGATCAGGCCGAAAAGCCAACTGGTTATTTTAATTGATTGCTTAGTTTTTAATTAATTCAAAACCGCCCGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATAtt                                                                                   <  1:2014069/145‑1 (MQ=255)
                                                              ggTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATAtt                                                                                   <  2:2029330/150‑1 (MQ=255)
                                                                tctaaaaCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTa                                                                                 <  1:204880/147‑1 (MQ=255)
                                                                tCGAAAACCAGCGATAAACCGGTGTGGATGGATCTGGCCGAAAAGGCAACTGGTGATTTTTATTGTTTGCTGTTTTTTGAATTCTTTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTa                                                                                 <  1:2927244/150‑1 (MQ=255)
                                                                 cGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGATTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAg                                                                                <  1:1013614/150‑1 (MQ=255)
                                                                  gAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGa                                                                               <  2:3543866/150‑1 (MQ=255)
                                                                  gAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATCAGGAAGATGTTCAGAGGAATATTTAGa                                                                               >  1:1050275/1‑150 (MQ=255)
                                                                     aaCCAGCTATAAACGGGTGTGGTTGGATCAGGCCGAAAAGGAAAGTGGTTATTTTTATTGATGGCTTAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACAt                                                                            <  1:161338/150‑1 (MQ=255)
                                                                      aCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATc                                                                           <  2:2051282/150‑1 (MQ=255)
                                                                       ccAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTTTAAAGGATATGTTGAGATGGATATTTAGaaaaag                                                                          >  1:40488/1‑145 (MQ=255)
                                                                         aGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGAAATCGtt                                                                        >  2:4087480/1‑150 (MQ=255)
                                                                           cGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTtat                                                                      <  1:3028818/150‑1 (MQ=255)
                                                                            gATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTtata                                                                     <  1:1066203/150‑1 (MQ=255)
                                                                              tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                   <  1:4688849/150‑1 (MQ=255)
                                                                              tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                   <  1:3483201/150‑1 (MQ=255)
                                                                              tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                   <  1:3481804/150‑1 (MQ=255)
                                                                              tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                   <  1:1092427/150‑1 (MQ=255)
                                                                               aaaCCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcaa                                                                  >  2:3515063/1‑148 (MQ=255)
                                                                                aaCCGGTTTGTATGGATCAGGCCTAAAAGGCAACTGGTTATTTTTATTGATTGCTGAGTTTTTAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCaa                                                                 <  1:1266666/150‑1 (MQ=255)
                                                                                aaCCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCaa                                                                 >  2:3327108/1‑150 (MQ=255)
                                                                                  ccGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATc                                                               <  1:2135868/150‑1 (MQ=255)
                                                                                   cGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCg                                                              <  1:4337815/150‑1 (MQ=255)
                                                                                    ggTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGa                                                             <  2:3892217/150‑1 (MQ=255)
                                                                                      tgtgGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGaaa                                                           <  2:3348412/150‑1 (MQ=255)
                                                                                        tggatggaTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCCGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTAAGAGGAATATTTAGACATCGTTATACCAATCGAAACa                                                         >  2:1810949/1‑150 (MQ=255)
                                                                                        tggatggaTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACa                                                         <  1:3266260/150‑1 (MQ=255)
                                                                                               atCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTc                                                  <  2:1374900/150‑1 (MQ=255)
                                                                                               atCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAACAATCGAAACAAATAtta                                                  >  1:2732195/1‑149 (MQ=255)
                                                                                               atCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTc                                                  >  1:2732132/1‑150 (MQ=255)
                                                                                                tCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTTAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCa                                                 <  1:1436673/150‑1 (MQ=255)
                                                                                                 cAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCAt                                                <  1:3802036/150‑1 (MQ=255)
                                                                                                  aGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGATGAATATTTAGACATCGTTATACCAATCGAAAAATATATTCATg                                               >  1:1536345/1‑150 (MQ=255)
                                                                                                     ccGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACAAATATTCATGaaa                                            >  1:4351376/1‑150 (MQ=255)
                                                                                                      cGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAat                                           <  2:2254291/150‑1 (MQ=255)
                                                                                                      cGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATAAGGAAGATGTTCAGAGGAATATTTTGACAACGTTATAACACTAGAAACAAATATTAAAGAAat                                           >  1:3727676/1‑150 (MQ=255)
                                                                                                        aaaaGGCAGGTGGTGTTTTTTATTGATTGGTGTTTTTTGATTACTTTGAAAACCGCCAGCGGAAAGGCTGGGGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAatat                                         <  1:2887779/150‑1 (MQ=255)
                                                                                                         aaaGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAatata                                        <  2:531609/150‑1 (MQ=255)
                                                                                                          aaGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATATAGACATCGTTATACCAATCGAAACATATATTCATGAAatatat                                       <  2:213260/150‑1 (MQ=255)
                                                                                                               aaCTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCCGCGGAAACGCTGGCGGTTTTTTGTATCATGAAGATGTTCAGAGGAATATTTAGACATCGTTATAACAATCGAAACATATATTCAAGTAATATATATaaa                                  >  1:373598/1‑150 (MQ=255)
                                                                                                                aCTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAat                                 <  1:4612852/150‑1 (MQ=255)
                                                                                                                aCTGGTGATTTGTATTGATGGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAat                                 <  2:3465037/150‑1 (MQ=255)
                                                                                                                  tGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATAATGAACATTTTAAGAGGAATATTTATAAATACGTATAACAAAAGAAACAAATATTAAAGAAAAATATATaaaaaa                               >  1:483693/1‑147 (MQ=255)
                                                                                                                     tGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATAtttt                            <  1:3349622/150‑1 (MQ=255)
                                                                                                                     tGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATAtttt                            <  2:4588692/150‑1 (MQ=255)
                                                                                                                     tGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATAtttt                            >  2:4505409/1‑150 (MQ=255)
                                                                                                                     tGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATAtttt                            >  2:3859539/1‑150 (MQ=255)
                                                                                                                         ttttATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTa                        <  2:25627/150‑1 (MQ=255)
                                                                                                                            tATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAAtt                     <  1:787678/150‑1 (MQ=255)
                                                                                                                            tATTGATTGCTGAGTTTTGAATTCAATGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAAtt                     <  1:787808/150‑1 (MQ=255)
                                                                                                                              ttgattgCTGATTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGt                   <  1:22179/150‑1 (MQ=255)
                                                                                                                              ttgattgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTACTAATTGt                   >  2:4351664/1‑150 (MQ=255)
                                                                                                                               tgattgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGtt                  <  2:4519691/150‑1 (MQ=255)
                                                                                                                                 attgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAACCGCTGGCGGATTATTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCt                <  2:1587370/150‑1 (MQ=255)
                                                                                                                                 attgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCt                <  1:608797/150‑1 (MQ=255)
                                                                                                                                 attgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATATAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCt                <  2:1587375/150‑1 (MQ=255)
                                                                                                                                  ttgCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCtt               <  2:2473662/150‑1 (MQ=255)
                                                                                                                                        aGTTTTTAATTCATTTAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGa         <  1:476515/150‑1 (MQ=255)
                                                                                                                                        aGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTTTAAGGGATATTTTAAGAGGAAAATTTATACAAAGTTTTAACAAAAGAAAAAAATATTAATTAAAAAAATATAAAAATTTTAATAATTTTtattatattt         >  1:478846/1‑146 (MQ=255)
                                                                                                                                         gTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGTTGTTAAGAGGAATATTTAGACCTCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTACTAATTGTTCTTATCTGAc        >  2:2456990/1‑150 (MQ=255)
                                                                                                                                         gTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATTAAATATATATAAATATTTTCCTAATTGTTCTTATCTGAc        >  2:3799760/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATAT  >  CP009273/724667‑724959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTC  >  CP009273/724664‑724962
                                                                                                                                                     |                                                                                                                                                     
GGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATT                                                                                                                                                       <  SRR19973082.3028385/150‑1 (MQ=60)
 GCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTC                                                                                                                                                      >  SRR19973082.2451730/1‑150 (MQ=60)
   GCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCAT                                                                                                                                                    <  SRR19973082.2240368/150‑1 (MQ=60)
      GCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGA                                                                                                                                                 <  SRR19973082.4528063/150‑1 (MQ=60)
      GCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGA                                                                                                                                                 <  SRR19973082.4528128/150‑1 (MQ=60)
         TTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAA                                                                                                                                              >  SRR19973082.902073/1‑150 (MQ=60)
         TTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAA                                                                                                                                              >  SRR19973082.902042/1‑150 (MQ=60)
               GCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCA                                                                                                                                        <  SRR19973082.1247377/150‑1 (MQ=60)
                   TTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGG                                                                                                                                    >  SRR19973082.2147840/1‑150 (MQ=60)
                     CTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAATCCGCCCGCGGAA                                                                                                                                  >  SRR19973082.2908951/1‑150 (MQ=60)
                     CTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAA                                                                                                                                  >  SRR19973082.2908797/1‑150 (MQ=60)
                       GGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAAC                                                                                                                                <  SRR19973082.3711746/150‑1 (MQ=60)
                       GGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAAC                                                                                                                                <  SRR19973082.3721902/150‑1 (MQ=60)
                         AAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGC                                                                                                                              >  SRR19973082.3225657/1‑150 (MQ=60)
                          ttgcccgcgccagctttttcagctacctgtttcgagtctgttcgaaaaccagagataaaggggtgtgtatttttctgtcggcaaagggaaatgttttTTTTTATTGTTTGCTTAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCT                                                                                                                             <  SRR19973082.3588463/53‑1 (MQ=52)
                           AGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTG                                                                                                                            <  SRR19973082.4343857/150‑1 (MQ=60)
                                  GCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTT                                                                                                                     <  SRR19973082.3493567/150‑1 (MQ=60)
                                  GCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTT                                                                                                                     <  SRR19973082.3493542/150‑1 (MQ=60)
                                  GCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTT                                                                                                                     <  SRR19973082.2759996/150‑1 (MQ=60)
                                   CCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT                                                                                                                    <  SRR19973082.3079276/150‑1 (MQ=60)
                                     CGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTT                                                                                                                  >  SRR19973082.1083825/1‑150 (MQ=60)
                                     CGCTTTTACAGCTACCTGCATCGCGTCGGGTCGAAAACCCGCTATAAACGGGTGTGTTTGTATCAGGCCGAAAAGGCAACTGTTTATTTTTATTTATTGCTTAGTTTTTAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTT                                                                                                                  <  SRR19973082.952385/150‑1 (MQ=60)
                                       CTTTTACAGATACCTGCATCGGGTCTGGTCTAAAACCTGCTATAAACCGGTTTGTATGTATCAGGCCTAAAAGGCAACTGTTTTTTTTTATTGATTGCTTTGTTTTTAATACATTTAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGT                                                                                                                <  SRR19973082.3514285/150‑1 (MQ=60)
                                         TTAACAGCTACCTGCATCGGGTCTGGTCTAAAACCAGCTATAAACGGGTGTGTATGGATCCGGCCGAAAAGGCAAGTGTTTATTTTTTTTGTTTGCTGATTTTTGATTTCATTTAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTAT                                                                                                              <  SRR19973082.4167984/150‑1 (MQ=60)
                                            cccGCTACCTGCATCGCGTCTGGTCGAAAACCAGCTATAAACCGGTGTGGTTGGATCAGGCCGAAATGGCAACTGTTTATTTTTATTGATTGCTGATTTTTTAATTCATTTAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAG                                                                                                           <  SRR19973082.3776704/147‑1 (MQ=60)
                                             CAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTttattatg                                                                                                          >  SRR19973082.25970/1‑142 (MQ=60)
                                              AGCTACCTGCATCGCGTCTGGTCGAAAACCAGCTATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGA                                                                                                         <  SRR19973082.1432826/150‑1 (MQ=60)
                                              AGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGA                                                                                                         >  SRR19973082.2688177/1‑150 (MQ=60)
                                                CTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGTATGGATCAGGCCGAAAAGGCAACTGGTTATTTTTATTGATTGCTGAGTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAG                                                                                                       <  SRR19973082.252729/150‑1 (MQ=60)
                                                CTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAG                                                                                                       <  SRR19973082.715278/150‑1 (MQ=60)
                                                  ACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCGGGCGGTTTTTTTTTTAAGGAAGAT                                                                                                     >  SRR19973082.3208004/1‑150 (MQ=60)
                                                     TGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTT                                                                                                  >  SRR19973082.329982/1‑150 (MQ=60)
                                                     TGCATCGCGTCTGGTCCAAAACCAGCGATAAACCGGTGTGGTTGGATCAGGCCGAAAAGGAAACTGGTGATTTTTATTGATTGCTGAGTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTATTTTGTATCAGGAAGATGTT                                                                                                  <  SRR19973082.1047532/150‑1 (MQ=60)
                                                      GCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTC                                                                                                 <  SRR19973082.4749489/150‑1 (MQ=60)
                                                      GCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTC                                                                                                 <  SRR19973082.4750145/150‑1 (MQ=60)
                                                           GCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGG                                                                                            <  SRR19973082.1129157/150‑1 (MQ=60)
                                                             GTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGCAGATGTTAAGAGGAA                                                                                          >  SRR19973082.97692/1‑150 (MQ=60)
                                                               CTGGTCGAAAACCAGCTATAAACGGGTGTGGTTGGATCCGGCCGAAACGGCACGTGGTTTTTTTTTTTGTTTGCTGTGTTTTGAATTCATTGAAAACCGCCCGCGGAAACGGTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATA                                                                                        <  SRR19973082.2395640/150‑1 (MQ=60)
                                                               CTGGTCGAAAACCAGCGATAAACCGGGGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATA                                                                                        <  SRR19973082.4220677/150‑1 (MQ=60)
                                                                TGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATAATGAAGATGTTAAGAGGAATAT                                                                                       >  SRR19973082.4596141/1‑150 (MQ=60)
                                                                TGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTAAGAGGAATAA                                                                                       >  SRR19973082.4596419/1‑150 (MQ=60)
                                                                 gttccAAAACCAGCTATAAACCGGTGTGTATGGATCAGGCCGAAAAGCCAACTGGTTATTTTAATTGATTGCTTAGTTTTTAATTAATTCAAAACCGCCCGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATT                                                                                      <  SRR19973082.2026803/145‑1 (MQ=60)
                                                                   TCTAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTA                                                                                    <  SRR19973082.206566/150‑1 (MQ=60)
                                                                   TCGAAAACCAGCGATAAACCGGTGTGGATGGATCTGGCCGAAAAGGCAACTGGTGATTTTTATTGTTTGCTGTTTTTTGAATTCTTTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTA                                                                                    <  SRR19973082.2945343/150‑1 (MQ=60)
                                                                    CGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGATTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAG                                                                                   <  SRR19973082.1020650/150‑1 (MQ=60)
                                                                    CGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAACATTTAG                                                                                   <  SRR19973082.2375144/150‑1 (MQ=60)
                                                                     GAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATCAGGAAGATGTTCAGAGGAATATTTAGA                                                                                  >  SRR19973082.1057511/1‑150 (MQ=60)
                                                                        AACCAGCTATAAACGGGTGTGGTTGGATCAGGCCGAAAAGGAAAGTGGTTATTTTTATTGATGGCTTAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACAT                                                                               <  SRR19973082.162747/150‑1 (MQ=60)
                                                                        AACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACAT                                                                               <  SRR19973082.4364737/150‑1 (MQ=60)
                                                                          CCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTttttaaaggatatgttgagatggatatttagaaaaag                                                                             >  SRR19973082.40981/1‑113 (MQ=60)
                                                                          CCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACCCAGGCGGTTTTTTttttaaggaatattttaagatgaattattttaaaaag                                                                             >  SRR19973082.41048/1‑113 (MQ=60)
                                                                              CGATAAACGGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTAT                                                                         <  SRR19973082.3047099/150‑1 (MQ=60)
                                                                              CGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTAT                                                                         <  SRR19973082.3047457/150‑1 (MQ=60)
                                                                               GATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATA                                                                        <  SRR19973082.1073516/150‑1 (MQ=60)
                                                                                 TAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACC                                                                      <  SRR19973082.4717123/150‑1 (MQ=60)
                                                                                 TAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACC                                                                      <  SRR19973082.3503156/150‑1 (MQ=60)
                                                                                 TAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACC                                                                      <  SRR19973082.3504560/150‑1 (MQ=60)
                                                                                 TAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACC                                                                      <  SRR19973082.1099837/150‑1 (MQ=60)
                                                                                 TAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACC                                                                      <  SRR19973082.4717131/150‑1 (MQ=60)
                                                                                   AACCGGTTTGTATGGATCAGGCCTAAAAGGCAACTGGTTATTTTTATTGATTGCTGAGTTTTTAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAA                                                                    <  SRR19973082.1275391/150‑1 (MQ=60)
                                                                                     CCGGTGTGGATGGATCCGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATC                                                                  <  SRR19973082.4543481/150‑1 (MQ=60)
                                                                                     CCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGTTTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATC                                                                  <  SRR19973082.2149447/150‑1 (MQ=60)
                                                                                      CGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCG                                                                 <  SRR19973082.1964842/150‑1 (MQ=60)
                                                                                      CGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCG                                                                 <  SRR19973082.4363939/150‑1 (MQ=60)
                                                                                           TGGATGGATCAGGCCGAAAAGGAAACTGTTTATTTTTATTGATTGCTGATTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACA                                                            <  SRR19973082.2452293/150‑1 (MQ=60)
                                                                                           TGGATGGATCAGGCCGAAAAGGAAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACA                                                            <  SRR19973082.3286382/150‑1 (MQ=60)
                                                                                           TGGATGGATCAGACCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACA                                                            <  SRR19973082.4483802/150‑1 (MQ=60)
                                                                                                  ATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTttttaaggaaaaatttgaaattaatatttttaaaaagttataaaaaaagaaaaaaaaaatc                                                     >  SRR19973082.91453/1‑89 (MQ=60)
                                                                                                  ATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAACAATCGAAACAAATATTA                                                     >  SRR19973082.2748817/1‑150 (MQ=60)
                                                                                                  ATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTA                                                     >  SRR19973082.2748754/1‑150 (MQ=60)
                                                                                                  ATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGGTTTTTttttaagggagatgttaagatgaaaaattataaaaaattataacaaaagaaaaaaatatta                                                     >  SRR19973082.91595/1‑89 (MQ=60)
                                                                                                   TCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTTAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCA                                                    <  SRR19973082.1446445/150‑1 (MQ=60)
                                                                                                   TCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCA                                                    <  SRR19973082.1446453/150‑1 (MQ=60)
                                                                                                    CAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCAT                                                   <  SRR19973082.3825506/150‑1 (MQ=60)
                                                                                                     AGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGATGAATATTTAGACATCGTTATACCAATCGAAAAATATATTCATG                                                  >  SRR19973082.1546481/1‑150 (MQ=60)
                                                                                                        CCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTttataaggaatattttaataggaaaatttataaaacgttataacaatcgaaacaaataataaataaa                                               >  SRR19973082.2108102/1‑83 (MQ=60)
                                                                                                        CCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACAAATATTCATGAAA                                               >  SRR19973082.4377546/1‑150 (MQ=60)
                                                                                                         CGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATAAGGAAGATGTTCAGAGGAATATTTTGACAACGTTATAACACTAGAAACAAATATTAAAGAAAT                                              >  SRR19973082.3750740/1‑150 (MQ=60)
                                                                                                          GAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTTTAAGGAAGATGTTaacaggaaaatttataaaaaggtaaaaacaaacaaacaaaaaataaataaaaa                                             >  SRR19973082.1660252/1‑97 (MQ=60)
                                                                                                           AAAAGGCAGGTGGTGTTTTTTATTGATTGGTGTTTTTTGATTACTTTGAAAACCGCCAGCGGAAAGGCTGGGGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATAT                                            <  SRR19973082.2905606/150‑1 (MQ=60)
                                                                                                            AAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATA                                           <  SRR19973082.3498806/150‑1 (MQ=60)
                                                                                                                  AACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCCGCGGAAACGCTGGCGGTTTTTTGTATCATGAAGATGTTCAGAGGAATATTTAGACATCGTTATAACAATCGAAACATATATTCAAGTAATATATATAAA                                     >  SRR19973082.375994/1‑150 (MQ=60)
                                                                                                                   ACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAAT                                    <  SRR19973082.4640839/150‑1 (MQ=60)
                                                                                                                     TGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTttataatgaacattttaagaggaatatttataaatacgtataacaaaagaaacaaatattaaagaaaaatatataaaaaa                                  >  SRR19973082.487131/1‑70 (MQ=60)
                                                                                                                     TGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCGGGCGGTTTTTTGTtttagggagagtttgagaggtataattataaaacgttataccaaaccaaaaaaaaattaaataaaaaaatataaaaaa                                  >  SRR19973082.105702/1‑72 (MQ=60)
                                                                                                                        TGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTT                               <  SRR19973082.3370331/150‑1 (MQ=60)
                                                                                                                               TATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATT                        <  SRR19973082.792711/150‑1 (MQ=60)
                                                                                                                               TATTGATTGCTGAGTTTTGAATTCAATGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATT                        <  SRR19973082.792841/150‑1 (MQ=60)
                                                                                                                                 TTGATTGCTGATTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGT                      <  SRR19973082.22485/150‑1 (MQ=60)
                                                                                                                                    ATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATCATGAAAATGTTAAGAGGAATATTTAGAaaaagatatacccaaacaaacatatattaatgaaaaatatataaaaattttaataatttttaa                   >  SRR19973082.3424248/1‑87 (MQ=60)
                                                                                                                                    ATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCT                   <  SRR19973082.613115/150‑1 (MQ=60)
                                                                                                                                       GCTGTGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTAT                <  SRR19973082.4043765/150‑1 (MQ=60)
                                                                                                                                       GCTGATTTTTTAATTCCTTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTAT                <  SRR19973082.2180409/150‑1 (MQ=60)
                                                                                                                                       GCTGATTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCGGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTAT                <  SRR19973082.489344/150‑1 (MQ=60)
                                                                                                                                           AGTTTTTAATTCATTTAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGA            <  SRR19973082.479887/150‑1 (MQ=60)
                                                                                                                                           AGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTttttaagggatattttaagaggaaaatttatacaaagttttaacaaaagaaaaaaatattaattaaaaaaatataaaaattttaataatttttattatattt            >  SRR19973082.482237/1‑48 (MQ=60)
                                                                                                                                           AGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGA            <  SRR19973082.479920/150‑1 (MQ=60)
                                                                                                                                           AGTTTTCAATCCACTCAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCATAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGA            <  SRR19973082.479778/150‑1 (MQ=60)
                                                                                                                                            GTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTttttaagggattttttgagatgaaaaattataaaaacttataacaaaacaaaaaaataataaataaaaatatataaaaaatttacaaatttttattttattta           >  SRR19973082.2479579/1‑47 (MQ=60)
                                                                                                                                             TTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTttattatgaaggttttcagaggaaaaattataaaacgttttaacaaaagaaacaaaaattcattaaaaataaataaaaattttaaaaaatttttttttatgaca          >  SRR19973082.560791/1‑46 (MQ=60)
                                                                                                                                                     TCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTC  <  SRR19973082.3450550/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTC  >  CP009273/724664‑724962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑