Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 724,813 A→T 92.9% intergenic (+17/‑226) ybfA → / → rhsC DUF2517 family protein/Rhs family putative polymorphic toxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273724,8130AT92.9% 560.3 / 22.5 192intergenic (+17/‑226)ybfA/rhsCDUF2517 family protein/Rhs family putative polymorphic toxin
Reads supporting (aligned to +/- strand):  ref base A (6/8);  new base T (74/104);  total (80/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 192 total aligned reads displayed.

CGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATAT  >  CP009273/724666‑724959
                                                                                                                                                   |                                                                                                                                                  
cgcTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCa                                                                                                                                                  <  1:3924866/150‑1 (MQ=255)
cgcTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCa                                                                                                                                                  <  1:1738405/150‑1 (MQ=255)
    gCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGa                                                                                                                                              <  2:1066490/150‑1 (MQ=255)
        ttCATGCTTTTCTGGAAAGACCGCGCACGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTGTAGTGATTGCGGTGTTTTGAATTCCTTGAAAAc                                                                                                                                          >  1:1425890/1‑150 (MQ=255)
         tCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGTAAAcc                                                                                                                                         <  2:933628/150‑1 (MQ=255)
         tCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAAcc                                                                                                                                         <  1:4258406/150‑1 (MQ=255)
         tCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAAcc                                                                                                                                         <  1:4256842/150‑1 (MQ=255)
          cATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAATCCg                                                                                                                                        >  1:4239490/1‑150 (MQ=255)
            tGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGcc                                                                                                                                      >  2:4077617/1‑150 (MQ=255)
            tGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGcc                                                                                                                                      <  1:2127684/150‑1 (MQ=255)
             gCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCa                                                                                                                                     <  2:313024/150‑1 (MQ=255)
             gCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCa                                                                                                                                     <  1:4099198/150‑1 (MQ=255)
             gCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCa                                                                                                                                     <  1:2402792/150‑1 (MQ=255)
              cTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAg                                                                                                                                    <  1:1216524/150‑1 (MQ=255)
                 ttCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCgg                                                                                                                                 <  1:2076168/150‑1 (MQ=255)
                       aaaGACCGCGCCCGCTTTTTCAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGTAATGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACTTTGATAACCGCCAGCGGAAACGc                                                                                                                           >  2:4308963/1‑150 (MQ=255)
                        aaGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGAGGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCt                                                                                                                          >  1:650658/1‑150 (MQ=255)
                         aGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGCAACGCTg                                                                                                                         >  1:3407161/1‑150 (MQ=255)
                         aGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGcgg                                                                                                                         >  1:4231898/1‑148 (MQ=255)
                         aGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGcgg                                                                                                                         >  1:4029491/1‑148 (MQ=255)
                         aGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTg                                                                                                                         <  1:4050196/150‑1 (MQ=255)
                         aGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTg                                                                                                                         >  2:3229591/1‑150 (MQ=255)
                          gACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAATCCGCCAGCGGAAACGCTgg                                                                                                                        >  2:1019106/1‑150 (MQ=255)
                                gcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATCAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAACCCCTGGCGGttt                                                                                                                  >  2:3797146/1‑150 (MQ=255)
                                gcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAATCCGCCAGCGGAAACGCTGGCGGttt                                                                                                                  >  2:1291765/1‑150 (MQ=255)
                                gcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGttt                                                                                                                  <  1:3393345/150‑1 (MQ=255)
                                     cTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGt                                                                                                             >  2:2126087/1‑150 (MQ=255)
                                       tttACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCTATAAACCGGTGTGGATGTATCAGGCCTAAAAGGAAACTGGTTATTTTTATTGATTGCTGAGTTTTTAATACATTTAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTAt                                                                                                           <  1:1210628/150‑1 (MQ=255)
                                        ttACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTTTTGATTGCTGATTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATc                                                                                                          <  1:2015496/150‑1 (MQ=255)
                                        ttACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATc                                                                                                          >  1:2888159/1‑150 (MQ=255)
                                        ttACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATc                                                                                                          >  2:1998540/1‑150 (MQ=255)
                                          aCAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAg                                                                                                        <  2:498297/150‑1 (MQ=255)
                                               tACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGa                                                                                                   <  1:596080/150‑1 (MQ=255)
                                                acgtGCATCGCGTCGGGTCTAAAACCAGCTATAAACCGGTGTGGATGTATCAGGCCGAAAAGGAAACTGTTTTTTTTTTTTGATTGCTGTTTTTTTAATTAATTTAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGAt                                                                                                  <  1:3739592/147‑1 (MQ=255)
                                                aCCTGCATCGCGTCTGGTCGAAAACCAGCTATAAACCGGTGTGTATGGATCAGGCCGAAAAGGCAACTGGTTATTTTTTTTGATTGCTGATTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGAt                                                                                                  <  1:1551698/150‑1 (MQ=255)
                                                   tGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGtt                                                                                               <  1:277980/150‑1 (MQ=255)
                                                     cATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCa                                                                                             <  1:3199395/150‑1 (MQ=255)
                                                           gTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGCTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCCGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGaa                                                                                       >  2:4000026/1‑150 (MQ=255)
                                                            tCTGGTCTACAACCACCTATAAACCGGTGTGGATGTATACGGCCGAACAGTCAACGGTTGATTTTGAGTGCTTGCTTAGTTTTGAATTAATGTAAAACGGCCAGCGGAAACGATGGCGTTTTTTTGTATCAGGAAGATGTTCAGAGGAat                                                                                      <  1:3076158/150‑1 (MQ=255)
                                                            tCTGGTCTAAAACCCGCGATAAACGGGTGTGGATGGATCAGGCCTAAAAGGCAAGTGGTGATTTTTTTTGATTGCTGAGTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAat                                                                                      <  1:871111/150‑1 (MQ=255)
                                                            tCTGGTCGAAAACCCGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAat                                                                                      <  1:3076212/150‑1 (MQ=255)
                                                            tCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAat                                                                                      <  1:4298853/150‑1 (MQ=255)
                                                             cTGGCCGAAAACCAGCGATAAACCGGTGTGGATGGATAAGGCCTAAAAGTCAACTGGTTTTTTTTATTGATTGCTGAGTTTTTATTTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAata                                                                                     <  1:3131602/150‑1 (MQ=255)
                                                               ggcccaaaaCCCGCTATAACCGGGGGTGGTTGGATCTGGCCTAAACGGCAACTGTTTTTTTTTTTTGTTGGCTGTTTTTTTAATTATTTTAAACCCGCCAGCGGAACCGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATAtt                                                                                   <  1:21018/145‑1 (MQ=255)
                                                                gTCGAAAACCAGCGATAAACGGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATAttt                                                                                  <  1:2745413/150‑1 (MQ=255)
                                                                      aaCCAGCGATAAACCGGTGTGTATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGATTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACAt                                                                            <  1:1931810/150‑1 (MQ=255)
                                                                             gATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGTTGTTCTGAGGAATTTTTAGACATAGTtatt                                                                     >  1:3896038/1‑149 (MQ=255)
                                                                             caTAAAACCGTTTGGATTGAACACGCCGAAAACGCAACATTTTATTTTTATTTATTGCATATTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTtata                                                                     <  2:2622969/149‑1 (MQ=255)
                                                                                aaaCCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCa                                                                  >  2:4295337/1‑150 (MQ=255)
                                                                                   ccGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATc                                                               <  1:260302/150‑1 (MQ=255)
                                                                                   ccGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATc                                                               >  2:3326859/1‑150 (MQ=255)
                                                                                     ggTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGa                                                             <  1:1674042/150‑1 (MQ=255)
                                                                                     ggTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGa                                                             >  2:3529008/1‑150 (MQ=255)
                                                                                      gtgtGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGaa                                                            >  2:2497058/1‑150 (MQ=255)
                                                                                        gtgGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAAAATTTAGACATCGTTATACCAATCGAAAc                                                          >  1:1798598/1‑150 (MQ=255)
                                                                                         tggatggaTCAGGCCGTAAAGGCAACTGTTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACa                                                         <  1:3949089/150‑1 (MQ=255)
                                                                                         tggatggaTCAGGCCGAAAAGGAAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACa                                                         <  1:1157789/150‑1 (MQ=255)
                                                                                          ggatggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACat                                                        >  2:639034/1‑150 (MQ=255)
                                                                                            atggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACatat                                                      <  2:3439777/150‑1 (MQ=255)
                                                                                              ggatCAGGCCGAAATGGAAAGTGGTGTTTTTTTTTGTTGGCTGTGTTTTTATTTCTTTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACatatat                                                    <  1:1099835/150‑1 (MQ=255)
                                                                                               gatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATAtt                                                   <  2:14687/150‑1 (MQ=255)
                                                                                                 tCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCa                                                 <  2:228008/150‑1 (MQ=255)
                                                                                                 tCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCa                                                 <  2:1935236/150‑1 (MQ=255)
                                                                                                 tCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCa                                                 >  2:4167881/1‑150 (MQ=255)
                                                                                                   aGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTTTAAGGAAGATGTTAAGAGGAATATTTATAAAAACTTATACCAATAGAAAAATATATTAAtt                                               >  1:1911261/1‑149 (MQ=255)
                                                                                                   aGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATAAGGCAGATTTTAAGAGGAAAATTTATAAAACGTTTTTAAAATAGAAACATATATTAATg                                               >  1:1839058/1‑150 (MQ=255)
                                                                                                     gCCGAAAAGGCAACTGTTTATTTTTTTTGATTGCTGAGTTTTGAATTAATTTAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGaa                                             <  1:4118716/150‑1 (MQ=255)
                                                                                                      ccGAAAAGGCAACGGGTGATTTTTATTGAGTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAAGCGAAACATATATTCATGaaa                                            <  2:4208695/150‑1 (MQ=255)
                                                                                                         aaaaGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCATGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAatat                                         >  2:2244821/1‑150 (MQ=255)
                                                                                                           aaGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAatatat                                       <  2:1657544/150‑1 (MQ=255)
                                                                                                            aGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGTTGTTCAGAGGAATATTTAGACAACGTTATACCAATCGAAACAAATATTCAATAAatatata                                      >  1:1717058/1‑150 (MQ=255)
                                                                                                                 aCTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGCTGTTCAGAGGAATATTTAGACATCGTTATACCAATCGTAACATATATTCATGAAATATATATAAat                                 >  2:1600334/1‑150 (MQ=255)
                                                                                                                 aCTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATTTTCAGAGGAATATTTATACATCGGTATACCAATCGAAACATATATTAATGAAataaatataaat                                 >  1:1064585/1‑150 (MQ=255)
                                                                                                                 aCTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAat                                 >  2:2089736/1‑150 (MQ=255)
                                                                                                                 aCTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAat                                 >  1:3259703/1‑150 (MQ=255)
                                                                                                                 aCTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCCCCAGCCGAAACCCTGGCGGGTTTTTTTATCAGGAAGATGTTAATATTAAAATTTATACAACGCTATAACCAACGAAAAAAAAATTAATTAAAAAAAAATaaaa                                 >  1:2704663/1‑149 (MQ=255)
                                                                                                                   tGGTGATTTTTATTGATTGCTGAGTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAatat                               <  2:666653/150‑1 (MQ=255)
                                                                                                                    ggTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATTTTCAGAGGAATATTTAGACATCGTTATACCAATAGAAACATATATTAATGAAATATATATAAATAtt                              >  2:2960266/1‑150 (MQ=255)
                                                                                                                        aTTTTTATTGCTTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTcc                          <  1:2034544/150‑1 (MQ=255)
                                                                                                                         tttttATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACACATATTCATGAAATATATATAAATATTTTCCt                         <  2:553053/150‑1 (MQ=255)
                                                                                                                            ttATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAAt                      <  2:755591/150‑1 (MQ=255)
                                                                                                                             tATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGAACTCGTTATACCAATCGAAAAATATATTCATGTAATATATATAAATATTTTACTAAtt                     >  2:3302820/1‑150 (MQ=255)
                                                                                                                               ttgattgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCGGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGt                   <  2:871253/150‑1 (MQ=255)
                                                                                                                                 gtttgCTGATTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTc                 <  1:1455055/148‑1 (MQ=255)
                                                                                                                                   ttgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCtt               <  1:4041580/150‑1 (MQ=255)
                                                                                                                                    tgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCTGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTa              <  2:4239490/150‑1 (MQ=255)
                                                                                                                                    tgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTa              <  1:111887/150‑1 (MQ=255)
                                                                                                                                     gCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATTTTAAGAGGAAAATTTAGACATCGTTATACCAATCGAAAAATAAATTAATGAAAAATATATAAATATTTTACTAATTTTtattat             >  1:1055189/1‑150 (MQ=255)
                                                                                                                                     gCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACACTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTACTAATTGTTCTTAt             >  2:850508/1‑150 (MQ=255)
                                                                                                                                       tGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCt           <  1:4270347/150‑1 (MQ=255)
                                                                                                                                        gAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTg          <  2:3309945/150‑1 (MQ=255)
                                                                                                                                        gAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTg          <  2:787620/150‑1 (MQ=255)
                                                                                                                                          gTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGAc        <  2:2014023/150‑1 (MQ=255)
                                                                                                                                            tttGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAg      <  1:3795794/150‑1 (MQ=255)
                                                                                                                                              tGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGat    <  2:844133/150‑1 (MQ=255)
                                                                                                                                              tGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGat    <  2:2888159/150‑1 (MQ=255)
                                                                                                                                               gAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTAATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGata   >  2:3235409/1‑150 (MQ=255)
                                                                                                                                               gAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATAAGGAAGATGTTGAGATGAAAATTTTGACATAGTTATAACAATAGAAACATATATTAATTAAATATATATAAATATTTTACTAATTTTTATTATATGTCAGata   >  1:4284957/1‑150 (MQ=255)
                                                                                                                                                aaTTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGatat  >  2:250698/1‑150 (MQ=255)
                                                                                                                                                aaTTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTAAGAGGAATATTTATACATAGTTATACCAATCGAAACATATATTCATTAAATATATATAAATATTTTACTAATTGTTCTTATCTGTCAGatat  >  2:249871/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATAT  >  CP009273/724666‑724959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATC  >  CP009273/724666‑724960
                                                                                                                                                   |                                                                                                                                                   
CGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCA                                                                                                                                                   <  SRR19973079.3944718/150‑1 (MQ=60)
CGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCA                                                                                                                                                   <  SRR19973079.1747621/150‑1 (MQ=60)
 GCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACAT                                                                                                                                                  <  SRR19973079.1200282/150‑1 (MQ=60)
        TTCATGCTTTTCTGGAAAGACCGCGCACGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTGTAGTGATTGCGGTGTTTTGAATTCCTTGAAAAC                                                                                                                                           >  SRR19973079.1433608/1‑150 (MQ=60)
         TCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACC                                                                                                                                          <  SRR19973079.4279944/150‑1 (MQ=60)
         TCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACC                                                                                                                                          <  SRR19973079.4278378/150‑1 (MQ=60)
         TCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACC                                                                                                                                          <  SRR19973079.4278373/150‑1 (MQ=60)
         TCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACC                                                                                                                                          <  SRR19973079.3207123/150‑1 (MQ=60)
          CATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAATCCG                                                                                                                                         >  SRR19973079.4260945/1‑150 (MQ=60)
            TGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCC                                                                                                                                       <  SRR19973079.614962/150‑1 (MQ=60)
            TGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCC                                                                                                                                       <  SRR19973079.2138676/150‑1 (MQ=60)
             GCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCA                                                                                                                                      <  SRR19973079.2415193/150‑1 (MQ=60)
             GCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCA                                                                                                                                      <  SRR19973079.4119980/150‑1 (MQ=60)
             GCTGTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCA                                                                                                                                      <  SRR19973079.4120013/150‑1 (MQ=60)
              CTTTTCTGGAAAGACCGCGCCCGCTTTAACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAG                                                                                                                                     <  SRR19973079.1223513/150‑1 (MQ=60)
                 TTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGG                                                                                                                                  <  SRR19973079.2087021/150‑1 (MQ=60)
                   CTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAA                                                                                                                                >  SRR19973079.4346461/1‑150 (MQ=60)
                        AAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGAGGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCT                                                                                                                           >  SRR19973079.654326/1‑150 (MQ=60)
                         AGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGCAACGCTG                                                                                                                          >  SRR19973079.3424747/1‑150 (MQ=60)
                         AGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAACCGCTG                                                                                                                          >  SRR19973079.3424743/1‑150 (MQ=60)
                         AGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTG                                                                                                                          <  SRR19973079.4070687/150‑1 (MQ=60)
                         AGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCGG                                                                                                                          >  SRR19973079.4049879/1‑150 (MQ=60)
                         AGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCGG                                                                                                                          >  SRR19973079.4049827/1‑150 (MQ=60)
                         AGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCGG                                                                                                                          >  SRR19973079.4253308/1‑150 (MQ=60)
                                GCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTT                                                                                                                   <  SRR19973079.3410844/150‑1 (MQ=60)
                                       TTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCTATAAACCGGTGTGGATGTATCAGGCCTAAAAGGAAACTGGTTATTTTTATTGATTGCTGAGTTTTTAATACATTTAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTAT                                                                                                            <  SRR19973079.1217597/150‑1 (MQ=60)
                                        TTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTTTTGATTGCTGATTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATC                                                                                                           <  SRR19973079.2026129/150‑1 (MQ=60)
                                        TTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATC                                                                                                           >  SRR19973079.2902755/1‑150 (MQ=60)
                                          ACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATCAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCCGCGGAAACGCTGGCGGTTTTTTGTTTCAG                                                                                                         >  SRR19973079.2668709/1‑150 (MQ=60)
                                               TACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGA                                                                                                    <  SRR19973079.599564/150‑1 (MQ=60)
                                                ACGTGCATCGCGTCGGGTCTAAAACCAGCTATAAACCGGTGTGGATGTATCAGGCCGAAAAGGAAACTGTTTTTTTTTTTTGATTGCTGTTTTTTTAATTAATTTAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGAT                                                                                                   <  SRR19973079.3758760/150‑1 (MQ=60)
                                                ACCTGCATCGCTTCTGGTCTAAAACCAGCTATAAACCGGTGTGTTTGTATCAGGCCGAAAAGGAAACTGGTTATTTTTATTGATTGCTGTTTTTTGAATAAATTGAAAACCGCCCGCGTAAACGCGGGCGGTTTTTTGTATCAGGAAGAT                                                                                                   <  SRR19973079.1497916/150‑1 (MQ=60)
                                                ACCTGCATCGCGTCTGGTCGAAAACCAGCTATAAACCGGTGTGTATGGATCAGGCCGAAAAGGCAACTGGTTATTTTTTTTGATTGCTGATTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGAT                                                                                                   <  SRR19973079.1560265/150‑1 (MQ=60)
                                                ACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGTTTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGAT                                                                                                   <  SRR19973079.3577117/150‑1 (MQ=60)
                                                  CTGCATCGCGTCGGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCCGGCCGAAAAGGCAAGTGTTTATTTTTATTGATTGCTGAGTTTTGAATTCATTTAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGT                                                                                                 <  SRR19973079.2226163/150‑1 (MQ=60)
                                                   TGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTT                                                                                                <  SRR19973079.279706/150‑1 (MQ=60)
                                                    GCATCGCGTCTGGTCGAAACCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCGGGCGGTTTTTTGTATCAGGAAGATGTTC                                                                                               <  SRR19973079.1220023/150‑1 (MQ=60)
                                                     CATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCA                                                                                              <  SRR19973079.3215722/150‑1 (MQ=60)
                                                     CATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCA                                                                                              <  SRR19973079.3216758/150‑1 (MQ=60)
                                                          CGTCGGGTCGAAAACCAGCGATAAACCGGTGGGGATGGATCAGGCCGAAAAGGCAAGTGGTGATTTTTTTTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGA                                                                                         <  SRR19973079.2726221/150‑1 (MQ=60)
                                                            TCTGGTCTACAACCACCTATAAACCGGTGTGGATGTATACGGCCGAACAGTCAACGGTTGATTTTGAGTGCTTGCTTAGTTTTGAATTAATGTAAAACGGCCAGCGGAAACGATGGCGTTTTTTTGTATCAGGAAGATGTTCAGAGGAAT                                                                                       <  SRR19973079.3092080/150‑1 (MQ=27)
                                                            TCTGGTCTAAAACCCGCGATAAACGGGTGTGGATGGATCAGGCCTAAAAGGCAAGTGGTGATTTTTTTTGATTGCTGAGTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAAT                                                                                       <  SRR19973079.876152/150‑1 (MQ=60)
                                                            TCTGGTCGAAAACCCGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAAT                                                                                       <  SRR19973079.3092135/150‑1 (MQ=60)
                                                            TCTGGTCGAAAACCAGCGATAAACCGGTGTGTATGGATCTGGCCGAAAAGGCAACTGTTGATTTTTATTGATTGCTGATTTTTGAATTCATTTAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAAT                                                                                       <  SRR19973079.3092119/150‑1 (MQ=60)
                                                            TCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAAT                                                                                       <  SRR19973079.4320523/150‑1 (MQ=60)
                                                            TCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAAT                                                                                       <  SRR19973079.3092214/150‑1 (MQ=60)
                                                             CTGGCCGAAAACCAGCGATAAACCGGTGTGGATGGATAAGGCCTAAAAGTCAACTGGTTTTTTTTATTGATTGCTGAGTTTTTATTTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATA                                                                                      <  SRR19973079.3147715/150‑1 (MQ=60)
                                                               GGTCCAAAACCAGCTATAAACGGGTGTGGATGTAACAGGCCCAAACGGCAACGGGTGTTTTTTATGGTTTGCTGATTTTTTAATTCATTGAAAACCGCCAGCGGAACCGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATT                                                                                    <  SRR19973079.1834681/150‑1 (MQ=60)
                                                                GTCGAAAACCAGCGATAAACGGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTT                                                                                   <  SRR19973079.2759574/150‑1 (MQ=60)
                                                                GTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTT                                                                                   <  SRR19973079.2449833/150‑1 (MQ=60)
                                                                     AAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCACCGGAAACGCGGGCGGTTTTTTttataatgaagaatttaagaggacaaatttgaca                                                                              >  SRR19973079.2938030/1‑116 (MQ=60)
                                                                      AACCAGCGATAAACCGGTGTGTATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGATTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACAT                                                                             <  SRR19973079.1942126/150‑1 (MQ=60)
                                                                      AACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTATACAT                                                                             >  SRR19973079.3994722/1‑150 (MQ=60)
                                                                          agaataccggtattgcgttcgtttccttgaaggAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTT                                                                         <  SRR19973079.716194/117‑1 (MQ=60)
                                                                          agaataccggtagtgcgttcgtttccttgaaggAAGGCAACTGGTGATTTTTATTGTTTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTT                                                                         <  SRR19973079.1193555/117‑1 (MQ=60)
                                                                             GATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGTTGTTCTGAGGAATTTTTAGACATAGTTATT                                                                      >  SRR19973079.3915812/1‑150 (MQ=60)
                                                                              ATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACCCTGGCGGTTTTTTTTATAAGGAAGAatttaaaatgaaaaattatacaaacgtataa                                                                     >  SRR19973079.3702080/1‑119 (MQ=60)
                                                                                   CCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATC                                                                <  SRR19973079.261972/150‑1 (MQ=60)
                                                                                     GGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATCAGGAAGATGTTCAGATGAAAATTTAGACAAAGTTATAACAAACGA                                                              >  SRR19973079.3818765/1‑150 (MQ=60)
                                                                                     GGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGA                                                              <  SRR19973079.1683072/150‑1 (MQ=60)
                                                                                     GGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGA                                                              <  SRR19973079.1683107/150‑1 (MQ=60)
                                                                                        GTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAAAATTTAGACATCGTTATACCAATCGAAAC                                                           >  SRR19973079.1807965/1‑150 (MQ=60)
                                                                                         TGGATGGATCAGGCCGTAAAGGCAACTGTTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACA                                                          <  SRR19973079.3968999/150‑1 (MQ=60)
                                                                                         TGGATGGATCAGGCCGAAAAGGAAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACA                                                          <  SRR19973079.1164443/150‑1 (MQ=60)
                                                                                         TGGATGGATCAGGCCCAAAAGGCAACTGGTTATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACA                                                          <  SRR19973079.2844738/150‑1 (MQ=60)
                                                                                          GGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGGAGATGTTCAGAGGcctatttagtcaaagttataccaatagaaaaaa                                                         >  SRR19973079.4104182/1‑117 (MQ=60)
                                                                                              GGATCAGGCCGAAATGGAAAGTGGTGTTTTTTTTTGTTGGCTGTGTTTTTATTTCTTTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATAT                                                     <  SRR19973079.1105991/150‑1 (MQ=60)
                                                                                                ATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTC                                                   <  SRR19973079.1376907/150‑1 (MQ=60)
                                                                                                ATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTC                                                   <  SRR19973079.2137076/150‑1 (MQ=60)
                                                                                                   AGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTttttaatgaaaattttaagatgtaaatttatacaaagttataaaaaaagaaaaaaaaattaaat                                                >  SRR19973079.1848081/1‑86 (MQ=60)
                                                                                                   AGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTttataagggagatttttagatggaaatttataaaaatgtttacaaaaagaaaaagatattaaat                                                >  SRR19973079.1848192/1‑86 (MQ=60)
                                                                                                   AGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTttataaggcagattttaagaggaaaatttataaaacgtttttaaaatagaaacatatattaatg                                                >  SRR19973079.1848741/1‑86 (MQ=60)
                                                                                                   AGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTTTAAGGAAGATGTTAAGAGGAATATTTAtaaaaacttataccaatagaaaaatatattaatt                                                >  SRR19973079.1921454/1‑116 (MQ=60)
                                                                                                   AGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATGTTGAGAGGAATATTTAGAAATAGTTATACCAATacaaaaaaaaattaatt                                                >  SRR19973079.1922206/1‑133 (MQ=60)
                                                                                                    GGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGGTTTTTGTATCAGGAAGATTTTGAGAGGAATATTTATTCATAGGTATACCAATCGAAACATATATTCATGA                                               >  SRR19973079.4142033/1‑150 (MQ=60)
                                                                                                     GCCGAAAAGGCAACTGTTTATTTTTTTTGATTGCTGAGTTTTGAATTAATTTAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA                                              <  SRR19973079.4139618/150‑1 (MQ=60)
                                                                                                     GCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA                                              <  SRR19973079.1940063/150‑1 (MQ=60)
                                                                                                       CGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAAT                                            <  SRR19973079.2098377/150‑1 (MQ=60)
                                                                                                       CGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGATATACCAATCGAAACATATATTCATGAAAT                                            <  SRR19973079.4296148/150‑1 (MQ=60)
                                                                                                        GAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATAAGGAAGATTTTCAAAGGAATATTTAtaaaaagttattacaaacgaaaaaaatattaaataaata                                           >  SRR19973079.3400105/1‑111 (MQ=60)
                                                                                                            AGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGTTGTTCAGAGGAATATTTAGACAACGTTATACCAATCGAAACAAATATTCAATAAATATATA                                       >  SRR19973079.1726219/1‑150 (MQ=60)
                                                                                                            AGCCAACGGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATA                                       <  SRR19973079.1111986/150‑1 (MQ=60)
                                                                                                               CAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTCAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAA                                    <  SRR19973079.773442/150‑1 (MQ=60)
                                                                                                                 attgtttattttaattgtgtgctgatattttAATACAATGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAAT                                  <  SRR19973079.1146405/119‑1 (MQ=60)
                                                                                                                 ACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATTTTCAGAGGAATATTTATACATCGGTATACCAATCGAAACATATATTAATGAAATAAATATAAAT                                  >  SRR19973079.1070400/1‑150 (MQ=60)
                                                                                                                 ACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAAT                                  >  SRR19973079.3276161/1‑150 (MQ=60)
                                                                                                                 ACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAAAATTTAGACATCGTTATAACAATCGAAACATATATTCATGAAATATATATAAAT                                  >  SRR19973079.1071332/1‑150 (MQ=60)
                                                                                                                 ACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCCCCAGCCGAAACCCTGGCGGGTTTTTTTATCAGGAAGATGTTaatattaaaatttatacaacgctataaccaacgaaaaaaaaattaattaaaaaaaaataaaa                                  >  SRR19973079.2718662/1‑88 (MQ=60)
                                                                                                                    GGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATGTTAATAGGAATATTTATAAATCGTTATACCAAACGAAAAAAATATTAATTAAATATATATAAAAATT                               >  SRR19973079.4283889/1‑150 (MQ=60)
                                                                                                                        ATTTTTATTGCTTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCC                           <  SRR19973079.2045241/150‑1 (MQ=60)
                                                                                                                        ATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGTAGATGTTCAGAGGAAAATTTATAAATCGGTATACCAATCGAAACATATATTCAAGAAATATATATAAATATTTTaa                           >  SRR19973079.1735886/1‑148 (MQ=60)
                                                                                                                           TTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAA                        <  SRR19973079.4278357/150‑1 (MQ=60)
                                                                                                                            TTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATAAGGAAGATGTTAAGAGGAATATTTATACAAAGTTATACCAATCGAAAAATATATTaaataaaaatatataaataatttacaaat                       >  SRR19973079.2054897/1‑121 (MQ=60)
                                                                                                                            TTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTAAAAGGAAGATGTTaagatgaaaattttgaaaaagttataacaaacgaaacataaattaaataaaaatatataaatattttaaaaat                       >  SRR19973079.4253909/1‑77 (MQ=60)
                                                                                                                                TGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCGGGCGGGTTTTTttttaaagaacattttcagagggaaatttataaaacgttatacaaaacgaaaaaaaaaataattaaaaatatataaaaaatttaataaatttt                   >  SRR19973079.4072712/1‑57 (MQ=60)
                                                                                                                                TGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACCCTGGCGGTTTTTTttttaaccgacaattttaaaagaaaaattatacaaacgtttaaaacaacaaaaaaaaaataaataaatataaataaaaaattttcaaattttt                   >  SRR19973079.4072706/1‑57 (MQ=60)
                                                                                                                                 GTTTGCTGATTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTC                  <  SRR19973079.1462951/150‑1 (MQ=60)
                                                                                                                                 GATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTC                  <  SRR19973079.3005211/150‑1 (MQ=60)
                                                                                                                                 GATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTC                  <  SRR19973079.1462683/150‑1 (MQ=60)
                                                                                                                                   TTGCTGATTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTT                <  SRR19973079.4062050/150‑1 (MQ=60)
                                                                                                                                   TTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTT                <  SRR19973079.2031049/150‑1 (MQ=60)
                                                                                                                                   TTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTT                <  SRR19973079.4062011/150‑1 (MQ=60)
                                                                                                                                    TGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTA               <  SRR19973079.112836/150‑1 (MQ=60)
                                                                                                                                     GCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATTTTAAGAGGAAAATTTAGACATCGTTATACCAATCGAAAAATAAATTAATGAAAAATATATAAATATTTTACTAATTTTTATTAT              >  SRR19973079.1060983/1‑150 (MQ=60)
                                                                                                                                     GCTGAGTTTTGAATTCATGGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTAT              <  SRR19973079.1621123/150‑1 (MQ=60)
                                                                                                                                      CTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTttataaggaacattttaacaggcaaaattagacatagttaaaccaaaagaaacaaagattaattaaatatatataaatattttactaatttttattata             >  SRR19973079.3785212/1‑51 (MQ=60)
                                                                                                                                       TGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCT            <  SRR19973079.4291940/150‑1 (MQ=60)
                                                                                                                                       TGAGTTTTGAATACATTGAAAACCGCCAGCGTAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCT            <  SRR19973079.2009125/150‑1 (MQ=60)
                                                                                                                                        GATTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTG           <  SRR19973079.1887126/150‑1 (MQ=60)
                                                                                                                                            TTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAG       <  SRR19973079.3815230/150‑1 (MQ=60)
                                                                                                                                               GAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATAAGGAAGATGTTGAGATGAAAATTTTGACATAGTTATAACAATAGAAACATATATTAATTAAATATATATAAATATTTTACTAATTTTTATTATATGTCAGATA    >  SRR19973079.4306588/1‑150 (MQ=60)
                                                                                                                                                AATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGATGAATATTTAGACATCGTTATACCAAACGAAACAAATATTCAAGAAATATATATAAATATTTTACAAATTGTTCTTATCTGACAGATAA   >  SRR19973079.3964513/1‑150 (MQ=60)
                                                                                                                                                 ATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATC  <  SRR19973079.324657/150‑1 (MQ=60)
                                                                                                                                                 ATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATC  <  SRR19973079.325186/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                   
CGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATC  >  CP009273/724666‑724960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑