Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 724,813 A→T 100% intergenic (+17/‑226) ybfA → / → rhsC DUF2517 family protein/Rhs family putative polymorphic toxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273724,8130AT99.4% 729.8 / ‑4.3 243intergenic (+17/‑226)ybfA/rhsCDUF2517 family protein/Rhs family putative polymorphic toxin
Reads supporting (aligned to +/- strand):  ref base A (0/3);  new base T (100/140);  total (100/143)
Fisher's exact test for biased strand distribution p-value = 2.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 243 total aligned reads displayed.

CTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGA  >  CP009273/724668‑724956
                                                                                                                                                 |                                                                                                                                               
cTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCa                                                                                                                                               >  1:2801531/1‑148 (MQ=255)
 tGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCAt                                                                                                                                              <  2:586119/148‑1 (MQ=255)
 tGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCAt                                                                                                                                              <  2:260450/148‑1 (MQ=255)
  gCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCAtt                                                                                                                                             >  2:2125120/1‑148 (MQ=255)
   ccTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTg                                                                                                                                            <  2:1578549/148‑1 (MQ=255)
   ccTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTg                                                                                                                                            >  1:2594482/1‑148 (MQ=255)
      ttCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGaaa                                                                                                                                         >  2:504373/1‑148 (MQ=255)
      ttCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGaaa                                                                                                                                         <  2:2117409/148‑1 (MQ=255)
       tCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGaaaa                                                                                                                                        <  2:2454562/148‑1 (MQ=255)
       tCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGaaaa                                                                                                                                        >  2:2691067/1‑148 (MQ=255)
        cATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAAc                                                                                                                                       >  2:2656845/1‑148 (MQ=255)
           gCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGc                                                                                                                                    >  2:816380/1‑148 (MQ=255)
              tttCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAg                                                                                                                                 >  1:3728091/1‑148 (MQ=255)
              tttCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAg                                                                                                                                 >  1:2692057/1‑148 (MQ=255)
              tttCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAg                                                                                                                                 >  1:3795599/1‑148 (MQ=255)
               ttCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAATCCGCCAGc                                                                                                                                >  2:3192459/1‑148 (MQ=255)
                 cTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCgg                                                                                                                              >  2:2713600/1‑148 (MQ=255)
                   ggAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGaa                                                                                                                            <  1:504373/148‑1 (MQ=255)
                    gAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGaaa                                                                                                                           >  2:2954708/1‑148 (MQ=255)
                    gAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGaaa                                                                                                                           >  2:2611992/1‑148 (MQ=255)
                      aaGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACg                                                                                                                         <  2:1195303/148‑1 (MQ=255)
                       aGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAAACGCCAGCGGAAACGc                                                                                                                        >  2:3147570/1‑148 (MQ=255)
                           cgcgCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGACACGCTGGc                                                                                                                    >  2:2370894/1‑148 (MQ=255)
                              gcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAAGTCATTGAAAACCGCCAGCGGAAACGCTGGCGGt                                                                                                                 <  2:657122/148‑1 (MQ=255)
                               cccGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGtt                                                                                                                <  1:1225948/148‑1 (MQ=255)
                                ccGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGttt                                                                                                               >  2:2534039/1‑148 (MQ=255)
                                  gCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGttttt                                                                                                             <  1:1560602/148‑1 (MQ=255)
                                      ttACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGTTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTa                                                                                                         <  2:427956/148‑1 (MQ=255)
                                        aCAGCTACCTGCATCGCGTCTGGTCTAAAACCAGCGATAAACCGGTGTGGATTGATCAGGCCTAAAAGGCAACTGGTTATTTTTATTGATTGCTGAGTTTTGAATAAATTGAAACCCGCCAGCGGAAACGCTGGCGGTTTTTTGTATc                                                                                                       <  1:3246140/148‑1 (MQ=255)
                                        aCAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATc                                                                                                       >  1:3787401/1‑148 (MQ=255)
                                            cTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACGGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTTATTTTTATTGATTGCTGTGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGa                                                                                                   <  1:2626137/148‑1 (MQ=255)
                                            cTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGa                                                                                                   <  2:3491807/148‑1 (MQ=255)
                                            cTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGa                                                                                                   <  2:1365211/148‑1 (MQ=255)
                                                  gCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGt                                                                                             <  2:2581786/148‑1 (MQ=255)
                                                  gCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGt                                                                                             >  2:2968925/1‑148 (MQ=255)
                                                  gCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGt                                                                                             >  2:1627820/1‑148 (MQ=255)
                                                  gCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGt                                                                                             <  2:1769535/148‑1 (MQ=255)
                                                        cgTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCCGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCagag                                                                                       >  1:1038559/1‑148 (MQ=255)
                                                           cTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCCGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGaa                                                                                    <  1:768745/148‑1 (MQ=255)
                                                             ggTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAata                                                                                  <  2:3700935/148‑1 (MQ=255)
                                                             ggTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAata                                                                                  <  2:3728091/148‑1 (MQ=255)
                                                              gTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAatat                                                                                 >  2:1928540/1‑148 (MQ=255)
                                                              gTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAatat                                                                                 <  1:1169578/148‑1 (MQ=255)
                                                                  aaaaCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAg                                                                             >  2:3625530/1‑148 (MQ=255)
                                                                    aaCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGAAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGAc                                                                           <  1:3705009/148‑1 (MQ=255)
                                                                     aCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACa                                                                          <  1:2710462/148‑1 (MQ=255)
                                                                     aCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACa                                                                          <  2:754060/148‑1 (MQ=255)
                                                                      ccAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACAt                                                                         >  2:502625/1‑148 (MQ=255)
                                                                          cGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATAGtt                                                                     >  2:202252/1‑148 (MQ=255)
                                                                           gATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTta                                                                    <  1:1605983/148‑1 (MQ=255)
                                                                                aCCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGGTTTTTGTGTGAGGAAGATGTTCAGAGGAATATTTAGAAATCGTTATACCa                                                               >  1:2603844/1‑148 (MQ=255)
                                                                                 ccGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCaa                                                              >  1:3451030/1‑148 (MQ=255)
                                                                                  cGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAAt                                                             <  2:3383838/148‑1 (MQ=255)
                                                                                   ggTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATc                                                            <  2:3647864/148‑1 (MQ=255)
                                                                                   ggTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATc                                                            >  2:1841469/1‑148 (MQ=255)
                                                                                      gtgGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTATACATCGTTATACCAATAGaa                                                         >  1:1900347/1‑148 (MQ=255)
                                                                                      gtgGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGaa                                                         <  2:3795599/148‑1 (MQ=255)
                                                                                          atggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGACACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACat                                                     <  2:3313145/148‑1 (MQ=255)
                                                                                            ggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACatat                                                   <  1:1768468/148‑1 (MQ=255)
                                                                                              atCCGGCCGAAAAGGCCACTGGTGATTTTTATGGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACatatat                                                 <  1:2201627/148‑1 (MQ=255)
                                                                                              atCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACatatat                                                 <  2:2801531/148‑1 (MQ=255)
                                                                                              atCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACatatat                                                 <  2:887951/148‑1 (MQ=255)
                                                                                                  ggCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATAAGGAATCTGTTGAGATGACAATTTATTCATCGTTATAACAATACAAAAAAATAttaaa                                             >  1:135196/1‑145 (MQ=255)
                                                                                                  ggCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCAt                                             <  1:728174/148‑1 (MQ=255)
                                                                                                   gCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATg                                            <  1:85538/148‑1 (MQ=255)
                                                                                                    ccGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATAATGAAGAAGTTAAGAGGAATATTTAGTCATAGGTATTACAAACGAAAAAAATATTCATGa                                           >  1:1432551/1‑148 (MQ=255)
                                                                                                    ccGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGa                                           <  2:1010018/148‑1 (MQ=255)
                                                                                                      gAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGaaa                                         <  1:2986469/148‑1 (MQ=255)
                                                                                                       aaaaGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATTTTGAGAGGAATATTTAGACATCGTTATACCAAACGAAACATATATTAATTaaaa                                        >  1:888335/1‑147 (MQ=255)
                                                                                                         aaGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATCATGAAGATGTTAAGAGGAAAATTTTTTAATCGTTATAACAAAAGAAACAAAAATTAATTAAaaat                                      >  1:2950845/1‑148 (MQ=255)
                                                                                                         aaGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAatat                                      <  1:1975665/148‑1 (MQ=255)
                                                                                                          aGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATAGTTATAACAATCGAAACATATATTAATTAAatata                                     >  2:1659206/1‑148 (MQ=255)
                                                                                                           ggCAACTGTTGATTTTTATTGATTGCTGATTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAatatat                                    <  1:1035718/148‑1 (MQ=255)
                                                                                                           ggCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTATACATCGTTATACCAATCGAAACATATATTCATGAAatatat                                    >  2:2279733/1‑148 (MQ=255)
                                                                                                            gCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAatatata                                   <  2:2858449/148‑1 (MQ=255)
                                                                                                                 tGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAat                              <  1:536935/148‑1 (MQ=255)
                                                                                                                  ggTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCCGCGGAAACGCTGGCGGTTTTTTTTATAAGGAAGTTGTTAAGATGCATATTTAGACAAAGTTATAACACTCGAAACCTAAAATAATGAAATATATAAAAata                             >  1:2632267/1‑148 (MQ=255)
                                                                                                                  ggTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCATAGGAATATTTAGACATCCTTATACCAAACGAAACAAATATTAATGAAAAATATATAAata                             >  1:3108388/1‑148 (MQ=255)
                                                                                                                  ggTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAata                             <  2:1746668/148‑1 (MQ=255)
                                                                                                                   gTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAatat                            <  1:1302494/148‑1 (MQ=255)
                                                                                                                   gTGATTTTTATTGATTGCTGAGTTTTGAAGTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAatat                            <  2:2943520/148‑1 (MQ=255)
                                                                                                                     gATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATAttt                          <  2:1110647/148‑1 (MQ=255)
                                                                                                                     gATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATAttt                          <  2:1049262/148‑1 (MQ=255)
                                                                                                                     gATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATAttt                          <  1:3175537/148‑1 (MQ=255)
                                                                                                                      aTTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATAtttt                         <  2:3353408/148‑1 (MQ=255)
                                                                                                                       tttttATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTc                        <  1:2118776/148‑1 (MQ=255)
                                                                                                                        ttttATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTTTAAGGAAGATGTTAATAGGAATATTTATACAAAGTTATACCAAAACAAAAATATATTCATTAAAAATATATAAATAtattaa                       >  1:3320599/1‑146 (MQ=255)
                                                                                                                        ttttATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTcc                       <  1:3356119/148‑1 (MQ=255)
                                                                                                                           tATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTaa                    <  1:3065705/148‑1 (MQ=255)
                                                                                                                             ttgattgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATAAGGAAGATTTTAAGAGGAATATTTATACATAGTTATAACAAACGAAACAAAAATTCATTAAATATATATAAATATTTTACTAAtt                  >  1:944576/1‑148 (MQ=255)
                                                                                                                             ttgattgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTATACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAAtt                  >  1:2857720/1‑148 (MQ=255)
                                                                                                                             ttgattgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTAAGAGGAATAATTATACAAAGTTATAACAATAGAAAAAAATATTAATTAAAAATATAAAAATATTTTACTaaat                  >  1:2860911/1‑146 (MQ=255)
                                                                                                                              tgattgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTg                 >  2:3153612/1‑148 (MQ=255)
                                                                                                                              tgattgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTg                 >  2:3804250/1‑148 (MQ=255)
                                                                                                                              tgattgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATTTTCATAGGAATATTTAGACATAGTTATACCAATCGAAACATATAATCATGAAATATATATAAATATTTTCATAAttt                 >  1:627075/1‑147 (MQ=255)
                                                                                                                               gattgCTGAGTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGt                <  1:3241950/148‑1 (MQ=255)
                                                                                                                               gattgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGt                <  1:449370/148‑1 (MQ=255)
                                                                                                                                  tgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATCAGGAAGATGTTAACAGGAAAATTTATAAAAAGTTTTAACAAAAGAAACATATAATAATTAAAAATATATAAAAATTTTAATAATttttat             >  1:2337471/1‑146 (MQ=255)
                                                                                                                                    cTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTa           <  2:944680/148‑1 (MQ=255)
                                                                                                                                             gAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGa  <  1:3406173/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGA  >  CP009273/724668‑724956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCT  >  CP009273/724666‑724961
                                                                                                                                                   |                                                                                                                                                    
CGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCA                                                                                                                                                    >  SRR19973069.894088/1‑150 (MQ=60)
CGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCA                                                                                                                                                    >  SRR19973069.2820129/1‑150 (MQ=60)
 GCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCAT                                                                                                                                                   <  SRR19973069.3195753/150‑1 (MQ=60)
 GCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCAT                                                                                                                                                   <  SRR19973069.3777921/150‑1 (MQ=60)
 GCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCAT                                                                                                                                                   <  SRR19973069.1875884/150‑1 (MQ=60)
   TGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTG                                                                                                                                                 >  SRR19973069.2612208/1‑150 (MQ=60)
   TGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTG                                                                                                                                                 >  SRR19973069.2611791/1‑150 (MQ=60)
   TGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTG                                                                                                                                                 <  SRR19973069.3668409/150‑1 (MQ=60)
      CTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAA                                                                                                                                              >  SRR19973069.837610/1‑150 (MQ=60)
       TTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAA                                                                                                                                             <  SRR19973069.3046982/150‑1 (MQ=60)
         TCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACC                                                                                                                                           >  SRR19973069.3417740/1‑150 (MQ=60)
          CATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGCTGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAAACG                                                                                                                                          >  SRR19973069.2956449/1‑150 (MQ=60)
              CTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAG                                                                                                                                      >  SRR19973069.2710096/1‑150 (MQ=60)
              CTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAG                                                                                                                                      >  SRR19973069.3820157/1‑150 (MQ=60)
              CTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAG                                                                                                                                      >  SRR19973069.3820197/1‑150 (MQ=60)
              CTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTGTATTGATTGCTGAGTTTTGAATTCATTGAAAAACGCCAG                                                                                                                                      >  SRR19973069.3752409/1‑150 (MQ=60)
                   CTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAA                                                                                                                                 >  SRR19973069.2877249/1‑150 (MQ=60)
                     GGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAAC                                                                                                                               <  SRR19973069.2723786/150‑1 (MQ=60)
                     GGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAAC                                                                                                                               <  SRR19973069.507984/150‑1 (MQ=60)
                     GGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAAC                                                                                                                               >  SRR19973069.2963052/1‑150 (MQ=60)
                                GCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTT                                                                                                                    <  SRR19973069.2250996/150‑1 (MQ=60)
                                 CCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT                                                                                                                   <  SRR19973069.3151398/150‑1 (MQ=60)
                                 CCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT                                                                                                                   <  SRR19973069.1234321/150‑1 (MQ=60)
                                    GCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTG                                                                                                                <  SRR19973069.1571256/150‑1 (MQ=60)
                                     CTTTTACCGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTTGATGGATCAGGCCGAAAAGGAAACTGGTTATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGT                                                                                                               <  SRR19973069.3099749/150‑1 (MQ=60)
                                       TTTACAGCTACCTTCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAAGTGGTTATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTAT                                                                                                             <  SRR19973069.3264928/150‑1 (MQ=60)
                                        TTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATC                                                                                                            >  SRR19973069.3812332/1‑150 (MQ=60)
                                        TTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATC                                                                                                            >  SRR19973069.3811966/1‑150 (MQ=60)
                                          ACAGCTCCCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACGGGTGTGGATGTATCAGGCCTAAAAGGCAACTGGTTATTTTTATTGATTGCTGTGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAG                                                                                                          <  SRR19973069.1351203/150‑1 (MQ=60)
                                          ACAGCTACCTGCATCGCGTCTGGTCTAAAACCAGCGATAAACCGGTGTGGATTGATCAGGCCTAAAAGGCAACTGGTTATTTTTATTGATTGCTGAGTTTTGAATAAATTGAAACCCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAG                                                                                                          <  SRR19973069.3267394/150‑1 (MQ=60)
                                              CTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACGGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTTATTTTTATTGATTGCTGTGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAG                                                                                                      <  SRR19973069.2643712/150‑1 (MQ=60)
                                              CTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGACTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAG                                                                                                      <  SRR19973069.1257949/150‑1 (MQ=60)
                                                ACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGAT                                                                                                    <  SRR19973069.2216207/150‑1 (MQ=60)
                                                      ATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAG                                                                                              >  SRR19973069.1182128/1‑150 (MQ=60)
                                                        CGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCCGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATGTTGAGAG                                                                                            >  SRR19973069.1045775/1‑150 (MQ=60)
                                                        CGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACCCGGGCGGTTTTTTTTATCAGGGAGATGTTaaaag                                                                                            >  SRR19973069.3741371/1‑145 (MQ=60)
                                                           GTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATAAGGAAGATGTTAAGAGGAA                                                                                         >  SRR19973069.3306281/1‑150 (MQ=60)
                                                           GTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCCGAAACGCGGGCGGTTTTTTGTAaaaggaccatgttaagtcgaa                                                                                         >  SRR19973069.3308852/1‑129 (MQ=60)
                                                             CGGGTCCAAAACCAGCGATAAACCGGTGTGGATGGATCCGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATA                                                                                       <  SRR19973069.774175/150‑1 (MQ=60)
                                                              TGGTCGAAAACCAGCGATAAACGGGGGTGGATGGATCAGGCCTAAAAGGCAAGTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATAT                                                                                      <  SRR19973069.718466/150‑1 (MQ=60)
                                                               GGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAACGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATT                                                                                     <  SRR19973069.1804422/150‑1 (MQ=60)
                                                                GTCGAAAACCAGCGATAAACCGGTGTGTATGGATCAGGCCGAAATGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTT                                                                                    <  SRR19973069.3472169/150‑1 (MQ=60)
                                                                GTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTT                                                                                    <  SRR19973069.1177692/150‑1 (MQ=60)
                                                                    AAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGAC                                                                                <  SRR19973069.2674653/150‑1 (MQ=60)
                                                                    AAAACCAGATATAAACCTGTTTGGATGGATCAGGCCTAAATGGCAACTGGTGATTTTTATTGATTGCTTAGTTTTGAATTCATTCAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGAC                                                                                <  SRR19973069.3757640/150‑1 (MQ=60)
                                                                      AACCAGCTATAAACCGGTGTGGATGGATCAGGCCTAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACAT                                                                              <  SRR19973069.3729456/150‑1 (MQ=60)
                                                                      AACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGAAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACAT                                                                              <  SRR19973069.3729217/150‑1 (MQ=60)
                                                                       ACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATC                                                                             <  SRR19973069.2728631/150‑1 (MQ=60)
                                                                        CCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCG                                                                            <  SRR19973069.2864128/150‑1 (MQ=60)
                                                                          AGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTT                                                                          <  SRR19973069.545616/150‑1 (MQ=60)
                                                                            CGATAAACGGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTTATTTTTATTGATTGCTTATTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTAT                                                                        <  SRR19973069.1323342/150‑1 (MQ=60)
                                                                            CGATAAACCGGTGTGGATGTATCAGGCCGAAACGGCAACTGGTTATTTTTATTGATTGCTGAGTTTTGAATTAATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTAT                                                                        <  SRR19973069.1404157/150‑1 (MQ=60)
                                                                             GATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATA                                                                       <  SRR19973069.1616788/150‑1 (MQ=60)
                                                                                AAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGGTTTTTGTGTGAGGAAGATGTTCAGAGGAATATTTAGAAATCGTTATACCA                                                                    >  SRR19973069.2621227/1‑150 (MQ=60)
                                                                                 AACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAA                                                                   >  SRR19973069.3473425/1‑150 (MQ=60)
                                                                                 AACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGAAATCGTTATACCAA                                                                   >  SRR19973069.3473406/1‑150 (MQ=60)
                                                                                  ACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTTATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAAT                                                                  <  SRR19973069.610130/150‑1 (MQ=60)
                                                                                  ACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAAT                                                                  <  SRR19973069.2258982/150‑1 (MQ=60)
                                                                                      GTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTATACATCGTTATACCAATAGAA                                                              >  SRR19973069.1913490/1‑150 (MQ=60)
                                                                                      GTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAAAATTTAGACATCGTTATACCAATCGAA                                                              >  SRR19973069.1913489/1‑150 (MQ=60)
                                                                                             TGGATCAGGCCGAAAAGGCAACTGGTTTTTTTTATTGTTTGCTGTTTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA                                                       <  SRR19973069.1303290/150‑1 (MQ=60)
                                                                                             TGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGATTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA                                                       <  SRR19973069.506229/150‑1 (MQ=60)
                                                                                             TGCATCAGGCCGAAAAGGCAACTGGTTATTTTTAGTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGGTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA                                                       <  SRR19973069.1695871/150‑1 (MQ=60)
                                                                                              gtaaCAGGCCGAAACGGCAAGTGGTGATTTTTTTTGATGGCTGATTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATAT                                                      <  SRR19973069.990770/146‑1 (MQ=60)
                                                                                              GGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATAT                                                      <  SRR19973069.1780931/150‑1 (MQ=60)
                                                                                               GATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTTTCAGGACGATGTTCAGAGGAATATTTAGAAATCGGTATACCAATCGAAACATATATT                                                     >  SRR19973069.2053776/1‑150 (MQ=60)
                                                                                               GATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATT                                                     <  SRR19973069.1941784/150‑1 (MQ=60)
                                                                                                ATCCGGCCGAAAAGGCCACTGGTGATTTTTATGGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTC                                                    <  SRR19973069.2216568/150‑1 (MQ=60)
                                                                                                  CAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTttataaggaatctgttgagatgacaatttattcatcgttataacaatacaaaaaaatattaaa                                                  >  SRR19973069.136454/1‑87 (MQ=60)
                                                                                                  CAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCAT                                                  <  SRR19973069.914456/150‑1 (MQ=60)
                                                                                                   AGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAAAATATATTCATG                                                 >  SRR19973069.3386489/1‑150 (MQ=60)
                                                                                                    GGCCGAAAAGGCAACTGGTTATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGA                                                <  SRR19973069.733383/150‑1 (MQ=60)
                                                                                                    GGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATAATGAAGAAGTTAAGAGGAATATTTAGtcataggtattacaaacgaaaaaaatattcatga                                                >  SRR19973069.1442549/1‑116 (MQ=60)
                                                                                                    GGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGA                                                <  SRR19973069.733319/150‑1 (MQ=60)
                                                                                                    GGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGA                                                <  SRR19973069.1556295/150‑1 (MQ=60)
                                                                                                     GCCGAAAAGGCAACTGGTGTTTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA                                               <  SRR19973069.86444/150‑1 (MQ=60)
                                                                                                       CGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATTTTGAGAGGAATATTTAGACATCGTTATACCAAACGAAACATATATTaattaaaa                                             >  SRR19973069.894472/1‑142 (MQ=60)
                                                                                                       CGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAAT                                             <  SRR19973069.2731789/150‑1 (MQ=60)
                                                                                                        GAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATA                                            <  SRR19973069.3006308/150‑1 (MQ=60)
                                                                                                        GAAAACGCAACTGGTTATTTTTATTGATTGCTGTGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATA                                            <  SRR19973069.2580174/150‑1 (MQ=60)
                                                                                                         AAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATCATGAAGATGTTAAGAGGAAaattttttaatcgttataacaaaagaaacaaaaattaattaaaaat                                           >  SRR19973069.2970427/1‑104 (MQ=60)
                                                                                                         AAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATAT                                           <  SRR19973069.161261/150‑1 (MQ=60)
                                                                                                          AAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATGTTAAGAGGAATATTTAttccaagttataacaatcgaaaaaaataataatgaaatata                                          >  SRR19973069.1135722/1‑109 (MQ=60)
                                                                                                           AAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATAT                                         <  SRR19973069.1989402/150‑1 (MQ=60)
                                                                                                             GGCAACTGTTGATTTTTATTGATTGCTGATTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATAT                                       <  SRR19973069.1042908/150‑1 (MQ=60)
                                                                                                             GGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATAT                                       <  SRR19973069.2709093/150‑1 (MQ=60)
                                                                                                             GGCAACTGGTGATTTTTATTGATGGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATAT                                       <  SRR19973069.2062907/150‑1 (MQ=60)
                                                                                                                  CTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCCGCGGAAACGCTGGCGGTTTTTTTTATAAGGAAGTTGTTAAGATGCATATTTAGACAAAGTTATAACACTCGAAACCTAAAATAATGAAATATATAAAAATA                                  >  SRR19973069.2649897/1‑150 (MQ=60)
                                                                                                                  CTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATAAGGGAGAAGTTGAGATGAATATTTAGACATAGTTATAacaaaagaaaaaaataataaattaaaatatataaata                                  >  SRR19973069.2649780/1‑113 (MQ=60)
                                                                                                                  CTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCATAGGAATATTTAGACATCCTTATACCAAACGAAACAAATATTAATGAAAAATATATAAATA                                  >  SRR19973069.3128835/1‑150 (MQ=60)
                                                                                                                  CTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATAGTTATACCAATCGAAACAAATATTCATTAAATATATATAAATA                                  >  SRR19973069.3128991/1‑150 (MQ=60)
                                                                                                                   TGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATAT                                 <  SRR19973069.540709/150‑1 (MQ=60)
                                                                                                                   TGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATAT                                 <  SRR19973069.540719/150‑1 (MQ=60)
                                                                                                                    GGTGATTTTTATTGTTTGCTGATTTTTGAATTCATTCAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATT                                <  SRR19973069.324135/150‑1 (MQ=60)
                                                                                                                     GTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTT                               <  SRR19973069.1311321/150‑1 (MQ=60)
                                                                                                                       GATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTC                             <  SRR19973069.3196201/150‑1 (MQ=60)
                                                                                                                        ATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTTTAAGGAAGATGTTAATAGGAATATTTATACAAAGTTATACCAAAACAAAAATATATTCATTAAAAATATATAAATATattaa                            >  SRR19973069.3342368/1‑145 (MQ=60)
                                                                                                                        ATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAAAATTTAGACAACGTTATAACAATCGAAAAAAATATTAATTAAAAATATATAAATATTTTaa                            >  SRR19973069.3343200/1‑148 (MQ=60)
                                                                                                                         TTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCT                           <  SRR19973069.2133377/150‑1 (MQ=60)
                                                                                                                          TTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTA                          <  SRR19973069.3378078/150‑1 (MQ=60)
                                                                                                                            TTATTGATTGCTGAGTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAAT                        <  SRR19973069.1045139/150‑1 (MQ=60)
                                                                                                                            TTATTGATTGCTGAGTTTTGAATTCATTGAAAACCCCCAGCGGAAACGCTGGCGGTTTTTTttaacatgaagaaggtaataggaaaaattatacaacgttaaaacaaaacaaaaaaaaaataaataaaaataaataaaaaatttacaaaa                        >  SRR19973069.844897/1‑61 (MQ=60)
                                                                                                                             TATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATAAGGAAGATTTTAAGAGGAATATTTATACATAGTTATAACAAACGAAACAAAAATTCATTAAATATATATAAATATTTTACTAATT                       >  SRR19973069.950943/1‑150 (MQ=60)
                                                                                                                             TATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTATACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATT                       >  SRR19973069.2876518/1‑150 (MQ=60)
                                                                                                                             TATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATT                       <  SRR19973069.3085973/150‑1 (MQ=60)
                                                                                                                             TATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATT                       <  SRR19973069.3085536/150‑1 (MQ=60)
                                                                                                                             TATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATT                       <  SRR19973069.2974321/150‑1 (MQ=60)
                                                                                                                             TATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATT                       <  SRR19973069.2629457/150‑1 (MQ=60)
                                                                                                                             TATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTAAGAGGAATAattatacaaagttataacaatagaaaaaaatattaattaaaaatataaaaatattttactaaat                       >  SRR19973069.2879718/1‑86 (MQ=60)
                                                                                                                             TATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCACCGGAAACGCTGGCGGTTTTTTGTATAAGGAATATTTTCAGAGGAATATTTAGACAACGTTATACCAATACAAACATATATTAATGAAATATATATAAATAATTTACTAAAT                       >  SRR19973069.950900/1‑150 (MQ=60)
                                                                                                                              ATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTTTAAGGAAGATTTTCAGAGGAAaaattatacaaacttataacaatcaaaaaaaataataatgaaaaaaaaaaaaaaaatttaaaaaatt                      >  SRR19973069.2623085/1‑83 (MQ=60)
                                                                                                                              ATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTG                      <  SRR19973069.499197/150‑1 (MQ=60)
                                                                                                                              ATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGTAGATTTTAAGAGGAAAATTTATACAACGTTATACCAAaagaaacaaaaaataattaaaaaaaaaaaaaaaatttactaattt                      >  SRR19973069.2623004/1‑105 (MQ=60)
                                                                                                                              ATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATTTTCATAGGAATATTTAGACATAGTTATACCAATCGAAACATATAATCATGAAATATATATAAATATTTTCATAATTT                      >  SRR19973069.631234/1‑150 (MQ=60)
                                                                                                                              ATTGATTGCTGAGTTTTGAATTCATTGAAAACCCCCACCGGAAACGCTGGCGGTTTTTTttataatgaacattttaataggaaaaattataaaaagttttaaccaaacaaaaataaaaaaattaaaaaaatataaatattttaataaaat                      >  SRR19973069.2608365/1‑59 (MQ=60)
                                                                                                                                 GATTGCTTAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTC                   <  SRR19973069.452607/150‑1 (MQ=60)
                                                                                                                                 GATTGCTGAGTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTC                   <  SRR19973069.3263169/150‑1 (MQ=60)
                                                                                                                                 GATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTC                   <  SRR19973069.452657/150‑1 (MQ=60)
                                                                                                                                  ATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTttatgaggtatattttaacatgaatatttataaaaagttataacaaaagaaaaaaataataaataaataaatataaaaattttaataaattttat                  >  SRR19973069.2766846/1‑55 (MQ=60)
                                                                                                                                  ATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATCAGGAAGATGTTaacaggaaaatttataaaaagttttaacaaaagaaacatataataattaaaaatatataaaaattttaataatttttat                  >  SRR19973069.2353375/1‑71 (MQ=60)
                                                                                                                                  ATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATAAGGAAGATTTTCAGAGGAAAATTTAtaaaaacttataacaaaaaaaaaaaaaattaaataaaaaaatataaataatttaaaaaattttat                  >  SRR19973069.1100790/1‑85 (MQ=60)
                                                                                                                                   TTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTT                 <  SRR19973069.822148/150‑1 (MQ=60)
                                                                                                                                         AGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTTTAAGGTAGATGTTCATAGGAATATTTATAAATCGTTATAACAATAGAAAAATATATTAATGAAATATATATAAATATTTTtataatttttattttatta           >  SRR19973069.1539429/1‑131 (MQ=60)
                                                                                                                                              TGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGAT      <  SRR19973069.3283658/150‑1 (MQ=60)
                                                                                                                                              TGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGAT      <  SRR19973069.1203075/150‑1 (MQ=60)
                                                                                                                                               GAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATA     <  SRR19973069.2754810/150‑1 (MQ=60)
                                                                                                                                               GAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATA     <  SRR19973069.3428382/150‑1 (MQ=60)
                                                                                                                                                 ATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATGAGGAAGAAGTTCAGAGGAAAAATTAGAAAACCTTATACCAATCGAAAAATAAATTAATTAAAAATATATAAAAATTTTacaaaattttattttatgaaagatatc   >  SRR19973069.3392668/1‑123 (MQ=44)
                                                                                                                                                 ATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTTTCAGGAAGATTTTCAGAGGAAAATTTATAAATCGGTATACCAAaccaaaaaaaaaataattaaatatataaaaataatttacaaaattttattatatgacagatata   >  SRR19973069.3392578/1‑86 (MQ=60)
                                                                                                                                                 ATTCATTGAAAACCGCCAGCGGAAACCCTGGCGGTTTTTTttattaagtagatttttaaatgcaaatatataaaaaggtttaaaaaaacaaaaaaaaataaaataaaaataaataaataaattaaaaaattttaatatattaaaaataat   >  SRR19973069.2676072/1‑40 (MQ=60)
                                                                                                                                                  TTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCT  <  SRR19973069.2139744/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                    
CGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCT  >  CP009273/724666‑724961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑