Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 724,813 A→T 92.4% intergenic (+17/‑226) ybfA → / → rhsC DUF2517 family protein/Rhs family putative polymorphic toxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273724,8130AT92.4% 617.9 / 25.5 202intergenic (+17/‑226)ybfA/rhsCDUF2517 family protein/Rhs family putative polymorphic toxin
Reads supporting (aligned to +/- strand):  ref base A (5/11);  new base T (71/115);  total (76/126)
Fisher's exact test for biased strand distribution p-value = 7.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 202 total aligned reads displayed.

CGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATAT  >  CP009273/724666‑724959
                                                                                                                                                   |                                                                                                                                                  
        ttCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAAc                                                                                                                                          >  2:3139036/1‑150 (MQ=255)
          cATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCg                                                                                                                                        <  1:4630034/150‑1 (MQ=255)
            tGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGcc                                                                                                                                      >  1:2764688/1‑150 (MQ=255)
              cTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAg                                                                                                                                    <  2:1307194/150‑1 (MQ=255)
                tttCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTTTTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCg                                                                                                                                  <  1:37004/150‑1 (MQ=255)
                tttCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCcgcg                                                                                                                                  >  1:2212657/1‑150 (MQ=255)
                     ggAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAAc                                                                                                                             <  2:2541920/150‑1 (MQ=255)
                      gAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACg                                                                                                                            <  2:1562846/150‑1 (MQ=255)
                       aaaGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGc                                                                                                                           >  1:4557417/1‑150 (MQ=255)
                            ccGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCg                                                                                                                      <  1:1503715/150‑1 (MQ=255)
                             cgcgCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCgg                                                                                                                     <  2:4217350/150‑1 (MQ=255)
                               cgcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAACACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGtt                                                                                                                   <  2:4490902/150‑1 (MQ=255)
                                   cGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGtttttt                                                                                                               <  1:2320846/150‑1 (MQ=255)
                                       tttACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTAt                                                                                                           <  2:861320/150‑1 (MQ=255)
                                       tttACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTAt                                                                                                           <  2:2320196/150‑1 (MQ=255)
                                           cAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCTATAAACCGGTGTGGATGTATCAGGCCGAAAAGGCAACTGGTTATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAgg                                                                                                       <  1:2201407/150‑1 (MQ=255)
                                            aGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGCTTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGa                                                                                                      >  2:3636322/1‑150 (MQ=255)
                                             gCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGaa                                                                                                     <  1:3876548/150‑1 (MQ=255)
                                                 ccTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATg                                                                                                 <  2:1304056/150‑1 (MQ=255)
                                                  cTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGt                                                                                                <  2:79239/150‑1 (MQ=255)
                                                   tGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGtt                                                                                               <  2:2099801/150‑1 (MQ=255)
                                                    gCAGCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTc                                                                                              <  1:2387206/150‑1 (MQ=255)
                                                     cATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCa                                                                                             <  1:1038260/150‑1 (MQ=255)
                                                     cATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCa                                                                                             >  2:461545/1‑150 (MQ=255)
                                                     cATCGCGTCTGGACGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCa                                                                                             <  1:1038405/150‑1 (MQ=255)
                                                      aTCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAACAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCag                                                                                            <  2:4378567/150‑1 (MQ=255)
                                                      aTCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTTTAAGGGAGATGttata                                                                                            >  1:1612146/1‑147 (MQ=255)
                                                       tCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCaga                                                                                           >  1:998671/1‑150 (MQ=255)
                                                           gTCTGGTCGAAAACCAGCGCTAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTGTGTATCGGGAAGAGGTTCAGAGGaa                                                                                       >  2:1685910/1‑150 (MQ=255)
                                                           gTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGaa                                                                                       >  2:1685898/1‑150 (MQ=255)
                                                             cTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAata                                                                                     <  2:3960104/150‑1 (MQ=255)
                                                                gTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAACGGCAACTGGTGATTTTTATTGTTTGCTGAGTTTTTAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATAttt                                                                                  <  1:258204/150‑1 (MQ=255)
                                                                 tCGAAAACCAGCTATAAACGGTTGTGGAGGTATCAGGCCGAAAAGGCAACTGGTGTTTTTTATTGATTGCTGTTTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTa                                                                                 <  1:1226762/150‑1 (MQ=255)
                                                                 tCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTa                                                                                 <  1:1791158/150‑1 (MQ=255)
                                                                   gAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGa                                                                               >  1:349915/1‑150 (MQ=255)
                                                                     aaaCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTTTCAGGTAGCTGTTTAGAGGAAAAATTATACa                                                                             >  1:4057478/1‑150 (MQ=255)
                                                                      aaCCAGCGATAAACGGGTGTGGTTGGATCAGGCCGAAAAGGCAACTGGTTATTTTTATTGTTGGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACAt                                                                            <  1:4490909/150‑1 (MQ=255)
                                                                      aaCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGTTTTTTTAATTCATTGAAAACCGCCTGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACAt                                                                            <  1:3535758/150‑1 (MQ=255)
                                                                      aaCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACAt                                                                            <  2:4154962/150‑1 (MQ=255)
                                                                        ccAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCg                                                                          <  2:3698718/150‑1 (MQ=255)
                                                                         cAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGt                                                                         >  2:4172454/1‑150 (MQ=255)
                                                                          aGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCATGAAGATGTTGAGAGGAATATTTAGAAAACGtt                                                                        >  2:2801689/1‑150 (MQ=255)
                                                                            cGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTtat                                                                      <  1:2232865/150‑1 (MQ=255)
                                                                             gATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTtata                                                                     <  2:3470358/150‑1 (MQ=255)
                                                                              aTAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAc                                                                    >  2:4313577/1‑150 (MQ=255)
                                                                               tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                   >  2:1957597/1‑150 (MQ=255)
                                                                               tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                   <  1:4278973/150‑1 (MQ=255)
                                                                               tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATGTTCAGAGGAATAATTAGACAACCTTATAcc                                                                   >  1:1124818/1‑150 (MQ=255)
                                                                                   ccGGTGTGGATGGATCAGGCCTAAAAGGAAACTGGTGATTTTTATTTATTGCTGATTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATc                                                               <  1:3775580/150‑1 (MQ=255)
                                                                                   ccGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCATGAAGATGTTAAGAGGAATATTTAGAAATAGTTATACCAATc                                                               >  1:740361/1‑150 (MQ=255)
                                                                                   ccGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATc                                                               <  2:2212657/150‑1 (MQ=255)
                                                                                   ccGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGAAATCGTTATACCAATc                                                               >  1:1866575/1‑150 (MQ=255)
                                                                                    gggTGTGGATGGATCAGGCCTAAAAGGAAACTGGTTATTTTTATTGATTGCTGAGTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCg                                                              <  1:3268256/149‑1 (MQ=255)
                                                                                    cGGTGTGGATGGATCAGGCCGAAAAGGCAACTGTTTATTTTTATTGATTGCTGAGTTTTGAATAAATTGAAAACCGCCAGCGTAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCg                                                              <  1:2064709/150‑1 (MQ=255)
                                                                                     ggTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGa                                                             >  1:2309629/1‑150 (MQ=255)
                                                                                       tgtgGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGaaa                                                           <  1:4771876/150‑1 (MQ=255)
                                                                                        gtgGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGTGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAAc                                                          <  2:4226969/150‑1 (MQ=255)
                                                                                        gtgGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGGTATACCAATCGAAAc                                                          >  1:3102090/1‑150 (MQ=255)
                                                                                         tggatggaTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTTAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACa                                                         <  1:2223722/150‑1 (MQ=255)
                                                                                            atggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACatat                                                      <  1:3178773/150‑1 (MQ=255)
                                                                                            atggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATAGAAACatat                                                      >  1:4341371/1‑150 (MQ=255)
                                                                                               gatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAATCCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATAtt                                                   <  2:1122450/150‑1 (MQ=255)
                                                                                               gatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATAtt                                                   <  1:4256294/150‑1 (MQ=255)
                                                                                                atCAGGCCGAAAAGGCAACTTGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTc                                                  <  2:3277181/150‑1 (MQ=255)
                                                                                                 tCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCa                                                 <  1:3044404/150‑1 (MQ=255)
                                                                                                  cAGGCCTAAAAGGCAACTGGTGTTTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCAt                                                <  1:279068/150‑1 (MQ=255)
                                                                                                      ccGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTAAGAGGAATATTTAGACATCGTTATACCAAACGAAACATATATTCATTaaa                                            >  2:1793214/1‑150 (MQ=255)
                                                                                                           aaGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAatatat                                       <  2:775462/150‑1 (MQ=255)
                                                                                                           aaGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAAAAAATATTAATGAAatatat                                       >  2:1256469/1‑150 (MQ=255)
                                                                                                              gCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAAtatatata                                    <  1:2658659/150‑1 (MQ=255)
                                                                                                              gCAACTGGTGATTTTTATTGATTGCTGAGTTTGGAATTCAGTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACCTCGTTATACCAATCGAAACATATATTCATGAAAtatatata                                    <  2:2857889/150‑1 (MQ=255)
                                                                                                               cAACGGGTGTTTTTTATTGATTGGTGTTTTTTGAATTCATTGAAAACCGCCAGCGGAACCGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATaa                                   <  1:3197964/150‑1 (MQ=255)
                                                                                                               aaaCTTGTGATTTTTATTGATTGATGATTTTTTAATAAATTTAAAACCTCCTGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATaa                                   <  1:1739016/149‑1 (MQ=255)
                                                                                                                aaCTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATaaa                                  <  2:3177922/150‑1 (MQ=255)
                                                                                                                    ggTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTTTAATGAATATGTTAATAGGAATATTTATAAATAGTTATAACAATAGAAAAATATATTAATGAAATATATATAAATAtt                              >  2:2799426/1‑150 (MQ=255)
                                                                                                                     gTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATAttt                             <  2:2314379/150‑1 (MQ=255)
                                                                                                                     gTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCGGGCGGTTTTTTGTAGCAGGAAGATGTTCAGAGGAAAATTTAGACATCGTTATACCCAACGAAACATATATTCATGAAATATATATAAATAttt                             >  1:1477419/1‑150 (MQ=255)
                                                                                                                       gATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATTAAATATATATAAATAtttta                           >  2:1558373/1‑149 (MQ=255)
                                                                                                                        aTTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTcc                          >  2:1709243/1‑150 (MQ=255)
                                                                                                                         tttttATTGATGGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCt                         <  1:2164464/150‑1 (MQ=255)
                                                                                                                          ttttATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTa                        <  2:1832395/150‑1 (MQ=255)
                                                                                                                          ttttATGGAGTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTa                        <  2:559501/150‑1 (MQ=255)
                                                                                                                              attgattgCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATAATGAAGATGTTAAGAGGAAAATTTTTAAAAAGTTATAACAAACGAAAAAAATATTAATTAAATAAATATAAAAAATTTACTAAttt                    >  1:1348711/1‑149 (MQ=255)
                                                                                                                              attgattgCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCGGGCGGTTTTTTTTTTGAGGTAGATTTTCAGATGAAAATTTAGAAAAAGTTTTACCAAAAGAAACAAATATTAATGAAAAATATATAAAAATTTTACTAAttt                    >  1:3758281/1‑149 (MQ=255)
                                                                                                                               ttgattgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATCAGGAAGTTGTTCATAGGTATATTTATAAATCGTTATACCAATAGAAAAATATAATCATTAAATATATAAAAATATTTTACTAAtttt                   >  2:891279/1‑148 (MQ=255)
                                                                                                                               ttgattgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATAGAAACAAAAATTCATTAAATATATATAAATATTTTCCTAAtttt                   >  2:891362/1‑148 (MQ=255)
                                                                                                                                tgattgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGtt                  <  2:4346057/150‑1 (MQ=255)
                                                                                                                                 gattgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTc                 <  1:461545/150‑1 (MQ=255)
                                                                                                                                  attgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATAATTAGACATCGTTATACAAAACGAAAAAAATATTAAAGAAATATATATAAATATTTTCCTAATTGTTCt                >  2:1632218/1‑150 (MQ=255)
                                                                                                                                       tGATTTTTTAATTCATTGAAACCCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCt           <  1:2801689/150‑1 (MQ=255)
                                                                                                                                       tGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTATTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCt           <  1:639527/150‑1 (MQ=255)
                                                                                                                                        gAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTg          <  2:4557417/150‑1 (MQ=255)
                                                                                                                                         aGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGa         <  2:3105993/150‑1 (MQ=255)
                                                                                                                                           ttttGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACa       <  1:4696887/150‑1 (MQ=255)
                                                                                                                                           ttttGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTAACAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACAAATATTCATGAAATATATATAAATATTTTCCTAAATGTTCTTATCTGAcc       >  1:792395/1‑149 (MQ=255)
                                                                                                                                            tttGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAg      <  1:1543160/150‑1 (MQ=255)
                                                                                                                                             ttGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGa     >  2:4252057/1‑150 (MQ=255)
                                                                                                                                             ttGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGa     >  2:1487695/1‑150 (MQ=255)
                                                                                                                                                aaTTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGatat  >  2:679651/1‑150 (MQ=255)
                                                                                                                                                aaTACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGatat  >  2:1984950/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATAT  >  CP009273/724666‑724959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATC  >  CP009273/724665‑724960
                                                                                                                                                    |                                                                                                                                                   
GCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTC                                                                                                                                                    <  SRR19973078.2225130/150‑1 (MQ=60)
       CTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAA                                                                                                                                             >  SRR19973078.3783108/1‑150 (MQ=60)
       CTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAA                                                                                                                                             >  SRR19973078.4167342/1‑150 (MQ=60)
        TTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAA                                                                                                                                            >  SRR19973078.1544280/1‑150 (MQ=60)
          TCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACC                                                                                                                                          <  SRR19973078.578820/150‑1 (MQ=60)
           CATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCG                                                                                                                                         <  SRR19973078.4656263/150‑1 (MQ=60)
             TGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCC                                                                                                                                       >  SRR19973078.2780141/1‑150 (MQ=60)
                 TTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTTTTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCG                                                                                                                                   <  SRR19973078.37434/150‑1 (MQ=60)
                 TTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCG                                                                                                                                   <  SRR19973078.37447/150‑1 (MQ=60)
                 TTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATGTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCCGCG                                                                                                                                   >  SRR19973078.2225577/1‑150 (MQ=60)
                      GGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAAC                                                                                                                              >  SRR19973078.4641607/1‑150 (MQ=60)
                        AAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGTAACGC                                                                                                                            >  SRR19973078.4583269/1‑150 (MQ=60)
                         AAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCT                                                                                                                           <  SRR19973078.1774384/150‑1 (MQ=60)
                             CCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCG                                                                                                                       <  SRR19973078.1512833/150‑1 (MQ=60)
                              CGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGG                                                                                                                      <  SRR19973078.4549014/150‑1 (MQ=60)
                                    ctaTTTTACAGCTACCTGCATCGGGTCTGGTCTAAAACCAGCTATAAACGGGTGTGTTTGGATCAGGCCTAAATGGAAACTGTTTTTTTTTATTGTTTGCTGTTTTTTTATTTAATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTT                                                                                                                <  SRR19973078.884608/147‑1 (MQ=41)
                                    CGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTT                                                                                                                <  SRR19973078.2334109/150‑1 (MQ=60)
                                    CGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGATTTTT                                                                                                                <  SRR19973078.2334104/150‑1 (MQ=60)
                                        TTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTAT                                                                                                            >  SRR19973078.2696243/1‑150 (MQ=60)
                                           ACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAG                                                                                                         <  SRR19973078.2237506/150‑1 (MQ=60)
                                            CAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCTATAAACCGGTGTGGATGTATCAGGCCGAAAAGGCAACTGGTTATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGG                                                                                                        <  SRR19973078.2214275/150‑1 (MQ=60)
                                             AGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGA                                                                                                       >  SRR19973078.4724183/1‑150 (MQ=60)
                                              GCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAA                                                                                                      <  SRR19973078.3898669/150‑1 (MQ=60)
                                               CTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAG                                                                                                     <  SRR19973078.1585687/150‑1 (MQ=60)
                                                 ACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGcaccgcgggcgggtttttgtttggggtcgag                                                                                                   >  SRR19973078.3952266/1‑119 (MQ=60)
                                                     GCAGCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTC                                                                                               <  SRR19973078.2400659/150‑1 (MQ=60)
                                                      CATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGTATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCA                                                                                              <  SRR19973078.1044755/150‑1 (MQ=60)
                                                      CATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGTTTATTTTTATTGATTGCTGAGTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCA                                                                                              <  SRR19973078.1045343/150‑1 (MQ=60)
                                                      CATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATCAGGGAGATGgtga                                                                                              >  SRR19973078.4631270/1‑146 (MQ=60)
                                                      CATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCA                                                                                              <  SRR19973078.1044682/150‑1 (MQ=60)
                                                      CATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTTTAAGGAAGATGTTTA                                                                                              >  SRR19973078.1221457/1‑150 (MQ=60)
                                                      CATCGCGTCTGGACGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCA                                                                                              <  SRR19973078.1044829/150‑1 (MQ=60)
                                                       ATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTttttgagggagcttttgag                                                                                             >  SRR19973078.1621814/1‑131 (MQ=60)
                                                       ATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTttttcgggatgatgttgat                                                                                             >  SRR19973078.1621811/1‑131 (MQ=60)
                                                       ATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTttttaatggagattttgag                                                                                             >  SRR19973078.1621772/1‑131 (MQ=60)
                                                       ATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTttaagggagatgttata                                                                                             >  SRR19973078.1621787/1‑133 (MQ=60)
                                                        TCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATAAGGAAGATGTTCAGA                                                                                            >  SRR19973078.1004917/1‑150 (MQ=60)
                                                           CGTCTGGTCTAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGA                                                                                         <  SRR19973078.2555420/150‑1 (MQ=60)
                                                           CGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTTTCTGGTATATTTTCAGAGGA                                                                                         >  SRR19973078.2601181/1‑150 (MQ=60)
                                                            GTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAA                                                                                        <  SRR19973078.345069/150‑1 (MQ=60)
                                                                 GTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAACGGCAACTGGTGATTTTTATTGTTTGCTGAGTTTTTAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTT                                                                                   <  SRR19973078.260108/150‑1 (MQ=60)
                                                                 GTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTT                                                                                   <  SRR19973078.3946528/150‑1 (MQ=60)
                                                                  TCTAAAACCAGCGATAACCCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGTTTATTTTTATTGATTGCGGATTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCGGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTA                                                                                  <  SRR19973078.1234687/150‑1 (MQ=60)
                                                                  TCGAAAACCAGCTATAAACGGTTGTGGAGGTATCAGGCCGAAAAGGCAACTGGTGTTTTTTATTGATTGCTGTTTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTA                                                                                  <  SRR19973078.1234265/150‑1 (MQ=60)
                                                                  TCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTA                                                                                  <  SRR19973078.1802010/150‑1 (MQ=60)
                                                                    GAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGA                                                                                >  SRR19973078.352131/1‑150 (MQ=60)
                                                                      AAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTttttcaggtagctgtttagaggaaaaattataca                                                                              >  SRR19973078.4080561/1‑116 (MQ=60)
                                                                      AAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAAAGCTGGCGGTTTTTTttataatgtatatgttaacttgaaaaatttgaca                                                                              >  SRR19973078.4081072/1‑116 (MQ=60)
                                                                       AACCAGCTATAACCGGGTGTGGATGTATCAGGCCTAAATGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTTAATTCATGGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACAT                                                                             <  SRR19973078.3254196/150‑1 (MQ=60)
                                                                       AACCAGCGATAAACGGGTGTGGTTGGATCAGGCCGAAAAGGCAACTGGTTATTTTTATTGTTGGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACAT                                                                             <  SRR19973078.4516331/150‑1 (MQ=60)
                                                                       AACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGTTTTTTTAATTCATTGAAAACCGCCTGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACAT                                                                             <  SRR19973078.3555753/150‑1 (MQ=60)
                                                                             CGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTAT                                                                       <  SRR19973078.2245852/150‑1 (MQ=60)
                                                                                TAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACC                                                                    <  SRR19973078.4303415/150‑1 (MQ=60)
                                                                                TAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACC                                                                    <  SRR19973078.4303244/150‑1 (MQ=60)
                                                                                TAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATGTTCAGAGGAATAATTAGACAACCTTATACC                                                                    >  SRR19973078.1131615/1‑150 (MQ=60)
                                                                                   ACGGGTGTGGTTGGATCCGGCCTAAAAGGAAAGTGGTGTTTTTTATTGTGTGCTGTTTTTTGAATTCATTGAAACCCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAAT                                                                 <  SRR19973078.3670048/150‑1 (MQ=60)
                                                                                   ACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAAT                                                                 <  SRR19973078.3156618/150‑1 (MQ=60)
                                                                                    CCGGTGTGGATGGATCAGGCCTAAAAGGAAACTGGTGATTTTTATTTATTGCTGATTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATC                                                                <  SRR19973078.3797257/150‑1 (MQ=60)
                                                                                    CCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATCAGGAAGATGTTCAGAGGAAAATTTATACATAGTTATAACAATC                                                                >  SRR19973078.4296296/1‑150 (MQ=60)
                                                                                    CCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCATGAAGATGTTAAGAGGAATATTTAGAAATAGTTATACCAATC                                                                >  SRR19973078.744817/1‑150 (MQ=60)
                                                                                    CCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACAACGTTATACCAATA                                                                >  SRR19973078.745708/1‑150 (MQ=60)
                                                                                    CCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGAAATCGTTATACCAATC                                                                >  SRR19973078.1877725/1‑150 (MQ=60)
                                                                                     GGGTGTGGATGGATCAGGCCTAAAAGGAAACTGGTTATTTTTATTGATTGCTGAGTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCG                                                               <  SRR19973078.3286790/150‑1 (MQ=60)
                                                                                     CGGTGTGGATGGATCAGGCCGAAAAGGCAACTGTTTATTTTTATTGATTGCTGAGTTTTGAATAAATTGAAAACCGCCAGCGTAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCG                                                               <  SRR19973078.2076875/150‑1 (MQ=60)
                                                                                     CGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGGTATACCAATCG                                                               >  SRR19973078.2793571/1‑150 (MQ=60)
                                                                                      GGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGA                                                              >  SRR19973078.2322861/1‑150 (MQ=60)
                                                                                        TGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAA                                                            <  SRR19973078.4798620/150‑1 (MQ=60)
                                                                                         GTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAAC                                                           <  SRR19973078.3550060/150‑1 (MQ=60)
                                                                                         GTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAAC                                                           >  SRR19973078.4417011/1‑150 (MQ=60)
                                                                                         GTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGGTATACCAATCGAAAC                                                           >  SRR19973078.3119557/1‑150 (MQ=60)
                                                                                          TGGATGTATCAGGCCGAAAAGGCAACTGGTTATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACA                                                          <  SRR19973078.2236544/150‑1 (MQ=60)
                                                                                          TGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTTAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACA                                                          <  SRR19973078.2236673/150‑1 (MQ=60)
                                                                                             ATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTTTCAGGCAGATGTTCAGAGGACTAGTTAGAAAACGGTATACCAATCGAAAacaat                                                       >  SRR19973078.4609100/1‑145 (MQ=60)
                                                                                             ATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATAT                                                       <  SRR19973078.3196457/150‑1 (MQ=60)
                                                                                             ATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATAGAAACATAT                                                       >  SRR19973078.4365828/1‑150 (MQ=60)
                                                                                                GATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATT                                                    <  SRR19973078.4280497/150‑1 (MQ=60)
                                                                                                 ATCAGGCCTAAAAGGCAACTGGTTTTTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTC                                                   <  SRR19973078.940570/150‑1 (MQ=60)
                                                                                                 ATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTC                                                   <  SRR19973078.2810286/150‑1 (MQ=60)
                                                                                                 ATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTC                                                   <  SRR19973078.285796/150‑1 (MQ=60)
                                                                                                 ATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTAAGAGGAATATTTAGACAACGTTATAacaaaagaaaaaaatatac                                                   >  SRR19973078.2216993/1‑131 (MQ=60)
                                                                                                  TCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCA                                                  <  SRR19973078.3061668/150‑1 (MQ=60)
                                                                                                   CAGGCCTAAAAGGCAACTGGTGTTTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCAT                                                 <  SRR19973078.281052/150‑1 (MQ=60)
                                                                                                       CCCAAAACGCAACTGGTGATTTTAATAGATTGCAGAGAATTCAATTCAATGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAA                                             <  SRR19973078.3864169/150‑1 (MQ=60)
                                                                                                        CGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAAT                                            <  SRR19973078.1855819/150‑1 (MQ=60)
                                                                                                            AAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATAAGGAAGATTTTAATAGGAATATTTAGACAAAGTTATAAAAATCGAAAAATATATTCATGAAATATAA                                        >  SRR19973078.2205484/1‑150 (MQ=60)
                                                                                                               GCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATA                                     <  SRR19973078.2673803/150‑1 (MQ=60)
                                                                                                               GCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATA                                     <  SRR19973078.1372198/150‑1 (MQ=60)
                                                                                                                CAACGGGTGTTTTTTATTGATTGGTGTTTTTTGAATTCATTGAAAACCGCCAGCGGAACCGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAA                                    <  SRR19973078.3215734/150‑1 (MQ=60)
                                                                                                                AAACTTGTGATTTTTATTGATTGATGATTTTTTAATAAATTTAAAACCTCCTGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAA                                    <  SRR19973078.1749611/150‑1 (MQ=60)
                                                                                                                  ACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAAT                                  <  SRR19973078.1492126/150‑1 (MQ=60)
                                                                                                                      GTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCGGGCGGTTTTTTGTAGCAGGAAGATGTTCAGAGGAAAATTTAGACATCGTTATACCCAACGAAACATATATTCATGAAATATATATAAATATTT                              >  SRR19973078.1486444/1‑150 (MQ=60)
                                                                                                                       TGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTT                             <  SRR19973078.2318395/150‑1 (MQ=60)
                                                                                                                          TTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCT                          <  SRR19973078.2177109/150‑1 (MQ=60)
                                                                                                                          TTTTTATTGATGGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCT                          <  SRR19973078.2177139/150‑1 (MQ=60)
                                                                                                                            TTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTttataatgaagattttaatatgaaaaattagacaacattataacaaaacaaacaaataataattaaataaatataaaaattttaataa                        >  SRR19973078.4668680/1‑62 (MQ=60)
                                                                                                                               ATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATAATGAAGATGTTAAGAGGAAaatttttaaaaagttataacaaacgaaaaaaatattaattaaataaatataaaaaatttactaattt                     >  SRR19973078.1357142/1‑83 (MQ=60)
                                                                                                                               ATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCGGGCGGTTTTTTTTTTGAGGTAGATTTTCAGATGAAAATTTAGAAAAAGTTTTACCAAAAGAAACAAATATTAATGAAAAATATATAAAAATTTTACTAATTT                     >  SRR19973078.3779863/1‑150 (MQ=44)
                                                                                                                                  GATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTC                  <  SRR19973078.464541/150‑1 (MQ=60)
                                                                                                                                        TGATTTTTTAATTCATTGAAACCCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCT            <  SRR19973078.2817286/150‑1 (MQ=60)
                                                                                                                                        TGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTATTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCT            <  SRR19973078.643629/150‑1 (MQ=60)
                                                                                                                                            TTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACA        <  SRR19973078.1969813/150‑1 (MQ=60)
                                                                                                                                            TTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACA        <  SRR19973078.4723390/150‑1 (MQ=60)
                                                                                                                                            TTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAAAATTTAGACAAAGTTATACCAATAGAAACAAATAATCAATAAATATATATAAATATTTTACTAATTTTTATTATATGACA        >  SRR19973078.3197321/1‑150 (MQ=60)
                                                                                                                                            TTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTAACAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACAAATATTCATGAAATATATATAAATATTTTCCTAAATGTTCTTATCTGACC        >  SRR19973078.797012/1‑150 (MQ=60)
                                                                                                                                             TTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATAAGGAAGATGTTCAGAGGAAAATTTATAAATAGTTATACCAATCGAAACAAAAAATCATGAAATATATATAAATATTTTACTAATTTTTATTATATGACAG       >  SRR19973078.2350865/1‑150 (MQ=60)
                                                                                                                                             TTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAG       <  SRR19973078.1552388/150‑1 (MQ=60)
                                                                                                                                               TGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGAT     <  SRR19973078.691531/150‑1 (MQ=60)
                                                                                                                                               TGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGAT     <  SRR19973078.3093472/150‑1 (MQ=60)
                                                                                                                                                  ATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATC  <  SRR19973078.2378415/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
GCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATC  >  CP009273/724665‑724960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑