Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 724,813 A→T 100% intergenic (+17/‑226) ybfA → / → rhsC DUF2517 family protein/Rhs family putative polymorphic toxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273724,8130AT98.8% 827.3 / 0.1 241intergenic (+17/‑226)ybfA/rhsCDUF2517 family protein/Rhs family putative polymorphic toxin
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base T (106/132);  total (107/134)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 241 total aligned reads displayed.

CGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATAT  >  CP009273/724666‑724959
                                                                                                                                                   |                                                                                                                                                  
cgcTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATGGCTGAGTTTTGAATTCa                                                                                                                                                  >  1:1140549/1‑150 (MQ=255)
  cTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCACCTGGTGATTTGTATTGATTGCTGAGGTTTGACTTCAtt                                                                                                                                                >  1:2871862/1‑150 (MQ=255)
  cTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCAtt                                                                                                                                                <  1:397508/150‑1 (MQ=255)
    gCCTTTCTTGCTTTTCTGGAAAGACCGCGCCCGATTTTACAGCTTCCTGCATCGCGTCTGGTCGAAAACAAGCGATTAACCGGTGTGGATGGATCAGGCAGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGa                                                                                                                                              <  1:359400/150‑1 (MQ=255)
    gCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGa                                                                                                                                              >  2:3575233/1‑150 (MQ=255)
    gCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGa                                                                                                                                              >  1:2443143/1‑150 (MQ=255)
      cTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGaaa                                                                                                                                            >  1:619185/1‑150 (MQ=255)
         tCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAAcc                                                                                                                                         <  2:2908381/150‑1 (MQ=255)
           aTGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGc                                                                                                                                       >  2:217757/1‑150 (MQ=255)
            tGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGcc                                                                                                                                      <  1:3383283/150‑1 (MQ=255)
              cTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAg                                                                                                                                    >  1:1708222/1‑150 (MQ=255)
              cTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAg                                                                                                                                    <  1:2335615/150‑1 (MQ=255)
                    tGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGaaa                                                                                                                              <  1:3235516/150‑1 (MQ=255)
                     ggAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAAc                                                                                                                             <  2:1254127/150‑1 (MQ=255)
                     ggAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAAc                                                                                                                             <  2:10353/150‑1 (MQ=255)
                      gAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACg                                                                                                                            <  2:968830/150‑1 (MQ=255)
                      gAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACg                                                                                                                            <  2:1577891/150‑1 (MQ=255)
                      gAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACg                                                                                                                            <  2:2628338/150‑1 (MQ=255)
                       aaaGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGc                                                                                                                           >  2:2798598/1‑150 (MQ=255)
                          gACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTgg                                                                                                                        <  1:1811425/150‑1 (MQ=255)
                           aCCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGc                                                                                                                       <  1:1329083/150‑1 (MQ=255)
                                gcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGttt                                                                                                                  >  1:3451145/1‑150 (MQ=255)
                                  ccGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGttttt                                                                                                                <  2:1951464/150‑1 (MQ=255)
                                   cGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGtttttt                                                                                                               <  2:3356784/150‑1 (MQ=255)
                                    gCTTTTACAGCTACCTGCATCGCGTCTGGTCGACAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTg                                                                                                              <  2:1771128/150‑1 (MQ=255)
                                     cTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGt                                                                                                             >  2:2924467/1‑150 (MQ=255)
                                      ttttACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTa                                                                                                            >  1:3879583/1‑150 (MQ=255)
                                          aCAGCGACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAg                                                                                                        <  2:4121269/150‑1 (MQ=255)
                                             gCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGaa                                                                                                     <  2:3994918/150‑1 (MQ=255)
                                              cTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAg                                                                                                    <  1:2944456/150‑1 (MQ=255)
                                                aCCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGAt                                                                                                  >  2:2749734/1‑150 (MQ=255)
                                                 ccTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTGTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATg                                                                                                 >  1:1989659/1‑150 (MQ=255)
                                                  cTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGt                                                                                                <  2:1470302/150‑1 (MQ=255)
                                                   tGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGtt                                                                                               >  2:1802922/1‑150 (MQ=255)
                                                   tGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATGtt                                                                                               >  2:1315106/1‑150 (MQ=255)
                                                     cATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCa                                                                                             <  1:417983/150‑1 (MQ=255)
                                                            tCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGTGGAat                                                                                      >  1:3924326/1‑150 (MQ=255)
                                                              tGGTCGATAAACAGCGATAAACCGGTGTGTATGGATCAGGCCGTAAAGGCAACTGGTGTTTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGATACGCTGGCGGTTTTTTGTATCAGTACGATGTTCAGAGGAatat                                                                                    <  1:3777818/150‑1 (MQ=255)
                                                              tGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAatat                                                                                    <  1:1017555/150‑1 (MQ=255)
                                                              tGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAatat                                                                                    <  2:885728/150‑1 (MQ=255)
                                                               ggTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGGTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATGTTCAGAGGAATAtt                                                                                   >  1:1395910/1‑150 (MQ=255)
                                                               ggTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATAtt                                                                                   <  2:2022691/150‑1 (MQ=255)
                                                                gTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATAttt                                                                                  <  1:1926937/150‑1 (MQ=255)
                                                                 tCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTa                                                                                 <  1:3684771/150‑1 (MQ=255)
                                                                  cGAAAACCAGCGATACACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCAGGCGGTTTTTTTTATAAGCAAGATGTTCAGAGGACTATTTAg                                                                                >  1:3160102/1‑150 (MQ=255)
                                                                   gAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGa                                                                               >  1:1275907/1‑150 (MQ=255)
                                                                    aaaaCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGAc                                                                              <  1:531186/150‑1 (MQ=255)
                                                                     aaaCCAGCGATAAACCGGTGTGGATGTATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACa                                                                             <  1:3238368/150‑1 (MQ=255)
                                                                     aaaCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACa                                                                             <  2:3953112/150‑1 (MQ=255)
                                                                      aaCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACAt                                                                            <  2:2220554/150‑1 (MQ=255)
                                                                       aCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATc                                                                           >  1:3115491/1‑150 (MQ=255)
                                                                          aGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCggt                                                                        >  2:628416/1‑148 (MQ=255)
                                                                          aGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGAGTGCTGAGTTTTTAATTCATTGAAAACCGCCAGCGGAAACGATGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGtt                                                                        <  2:3196646/150‑1 (MQ=255)
                                                                          aGCGATAAACCGGTGTGGATGGATCAGGCAGAAAATGCAACTGGTGATTTTTATTGATTGCTGATTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGtt                                                                        <  1:2191040/150‑1 (MQ=255)
                                                                            cGATCAACCGGTGTGGATGGATCAGGCCGAAAAGGAAACTGGTGAATATAATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACTCTGGCGGTTTTTTGTATCAGGAAGATGTTATGAGGAATATATAGACATCGTtat                                                                      >  2:3563539/1‑150 (MQ=255)
                                                                              aTAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAc                                                                    >  1:3755136/1‑150 (MQ=255)
                                                                               tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                   <  1:944944/150‑1 (MQ=255)
                                                                               tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                   <  2:2644710/150‑1 (MQ=255)
                                                                               tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                   <  2:1704541/150‑1 (MQ=255)
                                                                                aaaCCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCa                                                                  >  2:3340697/1‑150 (MQ=255)
                                                                                 aaCCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTTCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCaa                                                                 <  1:2661472/150‑1 (MQ=255)
                                                                                       tgtgGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGaaa                                                           <  2:717933/150‑1 (MQ=255)
                                                                                       tgtgGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGaaa                                                           >  2:1746689/1‑150 (MQ=255)
                                                                                           gatggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACata                                                       <  1:3793164/150‑1 (MQ=255)
                                                                                           gatggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACata                                                       <  2:1242628/150‑1 (MQ=255)
                                                                                           gatggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACata                                                       <  2:2953062/150‑1 (MQ=255)
                                                                                              ggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACatatat                                                    >  1:147931/1‑150 (MQ=255)
                                                                                              ggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACatatat                                                    <  2:2469054/150‑1 (MQ=255)
                                                                                              ggatCAGGCAGAAAAGGCAACTTGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGTAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACatatat                                                    <  2:542363/150‑1 (MQ=255)
                                                                                                 tCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCa                                                 <  1:2142006/150‑1 (MQ=255)
                                                                                                  cAGGCCGAAACGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCAt                                                <  1:854299/150‑1 (MQ=255)
                                                                                                  cAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCAt                                                >  2:3837560/1‑150 (MQ=255)
                                                                                                    ggCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGa                                              <  2:4143798/150‑1 (MQ=255)
                                                                                                    ggCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGa                                              <  1:208038/150‑1 (MQ=255)
                                                                                                    ggCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGa                                              <  1:208052/150‑1 (MQ=255)
                                                                                                     gCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGaa                                             <  1:2463208/150‑1 (MQ=255)
                                                                                                            aGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCTAAACATATATTCATGAAatatata                                      <  1:1603342/150‑1 (MQ=255)
                                                                                                              gCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAAtatatata                                    >  2:62575/1‑150 (MQ=255)
                                                                                                              gCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAAtatatata                                    >  2:2142686/1‑150 (MQ=255)
                                                                                                                  cTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAata                                >  2:2765830/1‑150 (MQ=255)
                                                                                                                    ggTGATTTGTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATAtt                              >  1:702459/1‑150 (MQ=255)
                                                                                                                     gTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATAAATAAATAttt                             >  1:931301/1‑150 (MQ=255)
                                                                                                                      tGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATAtttt                            <  1:1588242/150‑1 (MQ=255)
                                                                                                                        aTTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTcc                          >  1:3456405/1‑150 (MQ=255)
                                                                                                                        aTTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTcc                          <  2:3086732/150‑1 (MQ=255)
                                                                                                                            ttATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAAt                      >  1:2685987/1‑150 (MQ=255)
                                                                                                                              attgattgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAAttt                    >  1:1191427/1‑149 (MQ=255)
                                                                                                                              attgattgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTg                    <  1:2495766/150‑1 (MQ=255)
                                                                                                                                tgattgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGtt                  <  1:4029774/150‑1 (MQ=255)
                                                                                                                                 gattgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTc                 >  2:783065/1‑150 (MQ=255)
                                                                                                                                      cTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATc            >  1:3027447/1‑150 (MQ=255)
                                                                                                                                      cTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATc            >  2:4041062/1‑150 (MQ=255)
                                                                                                                                      cTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATc            <  1:1349648/150‑1 (MQ=255)
                                                                                                                                        gAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTg          >  2:3022906/1‑150 (MQ=255)
                                                                                                                                           ttttGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTATACATCGTTATACCAATCGAAACATATATTAAAGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACa       >  1:1878214/1‑150 (MQ=255)
                                                                                                                                            tttGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAg      <  2:3866524/150‑1 (MQ=255)
                                                                                                                                              tGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTGATTGTTCTTATCTGACAGat    >  2:335329/1‑150 (MQ=255)
                                                                                                                                              tGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGat    >  2:1842594/1‑150 (MQ=255)
                                                                                                                                              tGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGat    >  2:1410730/1‑150 (MQ=255)
                                                                                                                                                aaTTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACCTCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGatat  >  2:3491433/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATAT  >  CP009273/724666‑724959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGAT  >  CP009273/724664‑724957
                                                                                                                                                     |                                                                                                                                                
GGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATT                                                                                                                                                  >  SRR19973074.3092406/1‑150 (MQ=60)
 GCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTC                                                                                                                                                 <  SRR19973074.4028878/150‑1 (MQ=60)
 GCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTC                                                                                                                                                 <  SRR19973074.4028872/150‑1 (MQ=60)
  CGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATGGCTGAGTTTTGAATTCA                                                                                                                                                >  SRR19973074.1142716/1‑150 (MQ=60)
    CTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCACCTGGTGATTTGTATTGATTGCTGAGGTTTGACTTCATT                                                                                                                                              >  SRR19973074.2877190/1‑150 (MQ=60)
    CTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATT                                                                                                                                              >  SRR19973074.2877064/1‑150 (MQ=60)
    CTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATT                                                                                                                                              <  SRR19973074.398265/150‑1 (MQ=60)
      GCCTTTCTTGCTTTTCTGGAAAGACCGCGCCCGATTTTACAGCTTCCTGCATCGCGTCTGGTCGAAAACAAGCGATTAACCGGTGTGGATGGATCAGGCAGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGA                                                                                                                                            <  SRR19973074.360060/150‑1 (MQ=60)
      GCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATACACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGTATTCATTGA                                                                                                                                            >  SRR19973074.2471985/1‑150 (MQ=60)
      GCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGA                                                                                                                                            >  SRR19973074.2447663/1‑150 (MQ=60)
      GCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGA                                                                                                                                            >  SRR19973074.2471738/1‑150 (MQ=60)
        CTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAA                                                                                                                                          >  SRR19973074.620356/1‑150 (MQ=60)
            CATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCG                                                                                                                                      >  SRR19973074.1330499/1‑150 (MQ=60)
             ATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGC                                                                                                                                     >  SRR19973074.1234545/1‑150 (MQ=60)
             ATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGC                                                                                                                                     >  SRR19973074.1884196/1‑150 (MQ=60)
             ATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTGTTATTGATTGCTGAGTTTTGAATTCATTGTAATCCGC                                                                                                                                     >  SRR19973074.712312/1‑150 (MQ=60)
              TGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCC                                                                                                                                    <  SRR19973074.3389546/150‑1 (MQ=60)
               GCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCA                                                                                                                                   <  SRR19973074.3934096/150‑1 (MQ=60)
                CTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAG                                                                                                                                  >  SRR19973074.1711403/1‑150 (MQ=60)
                CTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAG                                                                                                                                  <  SRR19973074.2339971/150‑1 (MQ=60)
                      TGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAA                                                                                                                            <  SRR19973074.3241951/150‑1 (MQ=60)
                      TGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAA                                                                                                                            <  SRR19973074.3241503/150‑1 (MQ=60)
                        GAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACG                                                                                                                          >  SRR19973074.335108/1‑150 (MQ=60)
                          AAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCT                                                                                                                        >  SRR19973074.1920508/1‑150 (MQ=60)
                          AAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCT                                                                                                                        >  SRR19973074.2176051/1‑150 (MQ=60)
                          AAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCT                                                                                                                        >  SRR19973074.3805884/1‑150 (MQ=60)
                          AAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCT                                                                                                                        >  SRR19973074.3805989/1‑150 (MQ=60)
                          AAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCT                                                                                                                        >  SRR19973074.1115175/1‑150 (MQ=60)
                            GACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGG                                                                                                                      <  SRR19973074.1814871/150‑1 (MQ=60)
                             ACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGC                                                                                                                     <  SRR19973074.3311076/150‑1 (MQ=60)
                             ACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGC                                                                                                                     <  SRR19973074.1331571/150‑1 (MQ=60)
                                  GCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTT                                                                                                                >  SRR19973074.3457492/1‑150 (MQ=60)
                                  GCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTT                                                                                                                >  SRR19973074.3457503/1‑150 (MQ=60)
                                    CCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTT                                                                                                              >  SRR19973074.320231/1‑150 (MQ=60)
                                    CCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTT                                                                                                              >  SRR19973074.2453650/1‑150 (MQ=60)
                                      GCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTG                                                                                                            <  SRR19973074.1814915/150‑1 (MQ=60)
                                        TTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTA                                                                                                          >  SRR19973074.3886686/1‑150 (MQ=60)
                                                CTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAG                                                                                                  <  SRR19973074.2949928/150‑1 (MQ=60)
                                                 TACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGA                                                                                                 >  SRR19973074.133937/1‑150 (MQ=60)
                                                 TACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGA                                                                                                 <  SRR19973074.3010397/150‑1 (MQ=60)
                                                 TACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGA                                                                                                 >  SRR19973074.133808/1‑150 (MQ=60)
                                                   CCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTGTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATG                                                                                               >  SRR19973074.1993437/1‑150 (MQ=60)
                                                    CTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGT                                                                                              <  SRR19973074.3964278/150‑1 (MQ=60)
                                                       CATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCA                                                                                           <  SRR19973074.3205795/150‑1 (MQ=60)
                                                       CATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCA                                                                                           <  SRR19973074.418787/150‑1 (MQ=60)
                                                          CGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAG                                                                                        <  SRR19973074.310769/150‑1 (MQ=60)
                                                           GCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGG                                                                                       <  SRR19973074.2944922/150‑1 (MQ=60)
                                                              TCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGTGGAAT                                                                                    >  SRR19973074.3931542/1‑150 (MQ=60)
                                                              TCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAAT                                                                                    <  SRR19973074.1602510/150‑1 (MQ=60)
                                                                TGGTCGATAAACAGCGATAAACCGGTGTGTATGGATCAGGCCGTAAAGGCAACTGGTGTTTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGATACGCTGGCGGTTTTTTGTATCAGTACGATGTTCAGAGGAATAT                                                                                  <  SRR19973074.3784752/150‑1 (MQ=60)
                                                                TGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATAT                                                                                  <  SRR19973074.3784855/150‑1 (MQ=60)
                                                                TGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATAT                                                                                  <  SRR19973074.1019445/150‑1 (MQ=60)
                                                                 GGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGGTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATGTTCAGAGGAATATT                                                                                 >  SRR19973074.1398474/1‑150 (MQ=60)
                                                                 GGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATT                                                                                 <  SRR19973074.3682806/150‑1 (MQ=60)
                                                                  GTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTT                                                                                <  SRR19973074.1930624/150‑1 (MQ=60)
                                                                   TCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTA                                                                               <  SRR19973074.3691582/150‑1 (MQ=60)
                                                                    CGAAAACCAGCGATACACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCAGGCGGTTTTTTTTATAAGCAAGATGTTCAGAGGACTATTTAG                                                                              >  SRR19973074.3165908/1‑150 (MQ=60)
                                                                     GAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGA                                                                             >  SRR19973074.1278340/1‑150 (MQ=60)
                                                                     GAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGA                                                                             <  SRR19973074.3721278/150‑1 (MQ=60)
                                                                      AAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGAC                                                                            <  SRR19973074.532246/150‑1 (MQ=60)
                                                                       AAACCAGCGATAAACCGGTGTGGATGTATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACA                                                                           <  SRR19973074.3244364/150‑1 (MQ=60)
                                                                         ACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATC                                                                         <  SRR19973074.510116/150‑1 (MQ=60)
                                                                         ACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATC                                                                         >  SRR19973074.1152699/1‑150 (MQ=60)
                                                                         ACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATC                                                                         >  SRR19973074.3121207/1‑150 (MQ=60)
                                                                           CAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGT                                                                       <  SRR19973074.2218017/150‑1 (MQ=60)
                                                                            AGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTT                                                                      <  SRR19973074.2194864/150‑1 (MQ=60)
                                                                            AGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTAAGAGGAATATTTAGACATCGGT                                                                      >  SRR19973074.369327/1‑150 (MQ=60)
                                                                            AGCGATAAACCGGTGTGGATGGATCAGGCAGAAAATGCAACTGGTGATTTTTATTGATTGCTGATTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTT                                                                      <  SRR19973074.2195150/150‑1 (MQ=60)
                                                                             GCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGTTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTA                                                                     <  SRR19973074.4069973/150‑1 (MQ=60)
                                                                              CGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTAT                                                                    <  SRR19973074.3122443/150‑1 (MQ=60)
                                                                                ATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAC                                                                  >  SRR19973074.3762038/1‑150 (MQ=60)
                                                                                 TAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACC                                                                 <  SRR19973074.946740/150‑1 (MQ=60)
                                                                                   AACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTTCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAA                                                               <  SRR19973074.2666432/150‑1 (MQ=60)
                                                                                   AACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAA                                                               <  SRR19973074.65036/150‑1 (MQ=60)
                                                                                   AACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAA                                                               <  SRR19973074.579248/150‑1 (MQ=60)
                                                                                    ACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAAT                                                              <  SRR19973074.2504048/150‑1 (MQ=60)
                                                                                     cgggtgggtatgtttcaggcagaatagtCAACTGTTGATTTTTTTTGATTGCTTATTTTTGAATTAATTGAAAACCGACTGCGTAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATC                                                             <  SRR19973074.1822836/122‑1 (MQ=60)
                                                                                     CCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATC                                                             <  SRR19973074.3993515/150‑1 (MQ=60)
                                                                                       GGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGA                                                           >  SRR19973074.2986351/1‑150 (MQ=60)
                                                                                        GTGTTGTTGGTTCAGGCCGAAAATGGAACTGGTGTTTTTTTTTGATTGTTGAGTTTTGTATTCATTGAAAACCGCCAGCGGTAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAA                                                          <  SRR19973074.1447941/150‑1 (MQ=60)
                                                                                        GTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAA                                                          <  SRR19973074.1557061/150‑1 (MQ=60)
                                                                                            GGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACAT                                                      >  SRR19973074.3769436/1‑150 (MQ=60)
                                                                                            GGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACAT                                                      >  SRR19973074.3524148/1‑150 (MQ=60)
                                                                                            GGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGAAATAGTTATACCAATAGAAACAT                                                      >  SRR19973074.390379/1‑150 (MQ=60)
                                                                                             GATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATA                                                     <  SRR19973074.3800111/150‑1 (MQ=60)
                                                                                             GATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATA                                                     <  SRR19973074.1236495/150‑1 (MQ=60)
                                                                                               TGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA                                                   <  SRR19973074.2348042/150‑1 (MQ=60)
                                                                                               TGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA                                                   <  SRR19973074.2348058/150‑1 (MQ=60)
                                                                                                GGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATTTTCAGAGGAATATTTAGAAATCGTTATACCAATCGAAACATATAT                                                  >  SRR19973074.148472/1‑150 (MQ=60)
                                                                                                GGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATAT                                                  >  SRR19973074.148267/1‑150 (MQ=60)
                                                                                                GGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATAT                                                  <  SRR19973074.2679198/150‑1 (MQ=60)
                                                                                                 GATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATT                                                 >  SRR19973074.1978742/1‑150 (MQ=60)
                                                                                                   TCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCA                                               <  SRR19973074.2146032/150‑1 (MQ=60)
                                                                                                   TCAGGCCGAAAAGGCAACTAGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCA                                               <  SRR19973074.1363095/150‑1 (MQ=60)
                                                                                                   TCAGGCCGAAAAGGCAACTAGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCA                                               <  SRR19973074.676741/150‑1 (MQ=60)
                                                                                                    CAGGCCGAAACGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCAT                                              <  SRR19973074.855943/150‑1 (MQ=60)
                                                                                                    CAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCAT                                              <  SRR19973074.855984/150‑1 (MQ=60)
                                                                                                      GGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGA                                            <  SRR19973074.208487/150‑1 (MQ=60)
                                                                                                      GGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGA                                            <  SRR19973074.208474/150‑1 (MQ=60)
                                                                                                      GGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGA                                            <  SRR19973074.208468/150‑1 (MQ=60)
                                                                                                      GGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGA                                            <  SRR19973074.208473/150‑1 (MQ=60)
                                                                                                      GGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACACCGTTATACCAATCGAAACATATATTCATGA                                            <  SRR19973074.2145120/150‑1 (MQ=60)
                                                                                                       GCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA                                           <  SRR19973074.2467772/150‑1 (MQ=60)
                                                                                                          GAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATA                                        <  SRR19973074.1713963/150‑1 (MQ=60)
                                                                                                              AGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCTAAACATATATTCATGAAATATATA                                    <  SRR19973074.1606394/150‑1 (MQ=60)
                                                                                                              AGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATA                                    <  SRR19973074.4146312/150‑1 (MQ=60)
                                                                                                                 CAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAA                                 >  SRR19973074.1618502/1‑150 (MQ=60)
                                                                                                                 CAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAAAATATATTCATGAAATATATATAA                                 >  SRR19973074.1618497/1‑150 (MQ=60)
                                                                                                                      GGTGATTTGTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATT                            >  SRR19973074.703758/1‑150 (MQ=60)
                                                                                                                       GTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATAAATAAATATTT                           >  SRR19973074.933071/1‑150 (MQ=60)
                                                                                                                        TGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTT                          <  SRR19973074.1591263/150‑1 (MQ=60)
                                                                                                                          ATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCC                        >  SRR19973074.3462760/1‑150 (MQ=60)
                                                                                                                          ATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAATGAAGATGTTCAGAGGAATAATTAGACATCGTTATACCAATCGAAACATATATTCATTAAATATATATAAATATTTTCC                        >  SRR19973074.3462799/1‑150 (MQ=60)
                                                                                                                              TTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAAT                    >  SRR19973074.2690978/1‑150 (MQ=60)
                                                                                                                                ATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTT                  >  SRR19973074.1193700/1‑150 (MQ=60)
                                                                                                                                ATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTG                  <  SRR19973074.2500411/150‑1 (MQ=60)
                                                                                                                                  TGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTT                <  SRR19973074.4037221/150‑1 (MQ=60)
                                                                                                                                     TTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTT             <  SRR19973074.2846413/150‑1 (MQ=60)
                                                                                                                                        CTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATC          >  SRR19973074.3033046/1‑150 (MQ=60)
                                                                                                                                        CTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATC          <  SRR19973074.1352160/150‑1 (MQ=60)
                                                                                                                                        CTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATC          >  SRR19973074.938527/1‑150 (MQ=60)
                                                                                                                                         TGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATCTTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCT         <  SRR19973074.2058245/150‑1 (MQ=60)
                                                                                                                                           AGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGA       >  SRR19973074.3427525/1‑150 (MQ=60)
                                                                                                                                             TTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTATACATCGTTATACCAATCGAAACATATATTAAAGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACA     >  SRR19973074.1881806/1‑150 (MQ=60)
                                                                                                                                              TTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAG    <  SRR19973074.857180/150‑1 (MQ=60)
                                                                                                                                                TGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTttataagggagatggtcattggaaattttagaccacgttataacaaacgtaaaaataattcatgaaatatatataaaaaatttacaaattgttattttaataccgaa  >  SRR19973074.340470/1‑43 (MQ=60)
                                                                                                                                                     |                                                                                                                                                
GGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGAT  >  CP009273/724664‑724957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑