Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 843,299 C→A 41.1% W54C (TGG→TGT)  glnH ← glutamine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273843,2990CA41.1% 193.1 / 237.0 189W54C (TGG→TGT) glnHglutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (52/59);  new base A (28/50);  total (80/109)
Fisher's exact test for biased strand distribution p-value = 1.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 193 total aligned reads displayed.

AGAAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTT  >  CP009273/843151‑843445
                                                                                                                                                    |                                                                                                                                                  
 gaAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCca                                                                                                                                                  <  2:23167/150‑1 (MQ=255)
 gaAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCca                                                                                                                                                  <  2:22351/150‑1 (MQ=255)
  aaaTCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACG‑TTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGcacaca                                                                                                                                                <  2:320470/150‑1 (MQ=255)
  aaaTCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACG‑TTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGcacaca                                                                                                                                                <  2:580725/150‑1 (MQ=255)
       gatcgCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATc                                                                                                                                            <  2:2600044/150‑1 (MQ=255)
          cgCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAAc                                                                                                                                         >  2:2852594/1‑150 (MQ=255)
          cgCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAAc                                                                                                                                         <  1:720460/150‑1 (MQ=255)
          cgCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAAc                                                                                                                                         >  2:3765580/1‑150 (MQ=255)
             ttttttACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTc                                                                                                                                      <  1:566705/150‑1 (MQ=255)
                  tACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGc                                                                                                                                 <  1:3251403/150‑1 (MQ=255)
                    cGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACAGAAATCCAAAGGCGTCAGTTAGTAATACAGCTTCAGCTCTTTATCGATGGCAGCCCACAGATCAACGTCAAAGccc                                                                                                                               >  1:824090/1‑150 (MQ=255)
                     gCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTTCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCca                                                                                                                              >  2:1421441/1‑150 (MQ=255)
                       tcgtcgGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCcaca                                                                                                                            <  1:2902881/150‑1 (MQ=255)
                        cgtcgGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACat                                                                                                                           >  1:4214203/1‑150 (MQ=255)
                        cgtcgGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACat                                                                                                                           >  1:4216465/1‑150 (MQ=255)
                         gtcgGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACata                                                                                                                          >  2:4733400/1‑150 (MQ=255)
                         gtcgGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACata                                                                                                                          <  2:2442471/150‑1 (MQ=255)
                          tcgGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACatat                                                                                                                         <  2:36697/150‑1 (MQ=255)
                           cgGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATAtt                                                                                                                        <  2:1140360/150‑1 (MQ=255)
                           cgGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTGTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATAtt                                                                                                                        >  1:147408/1‑150 (MQ=255)
                            ggTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATAttt                                                                                                                       >  2:4742692/1‑150 (MQ=255)
                            ggTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTACCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATAttt                                                                                                                       <  2:3490885/150‑1 (MQ=255)
                             gTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTa                                                                                                                      <  1:3115910/150‑1 (MQ=255)
                                aTGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCg                                                                                                                   <  1:4603750/150‑1 (MQ=255)
                                aTGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCg                                                                                                                   <  1:2539674/150‑1 (MQ=255)
                                  ggTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGcc                                                                                                                 >  2:1865131/1‑150 (MQ=255)
                                  ggTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGcc                                                                                                                 <  2:1631089/150‑1 (MQ=255)
                                  ggTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGcc                                                                                                                 <  1:3946780/150‑1 (MQ=255)
                                  ggTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGcc                                                                                                                 >  1:595678/1‑150 (MQ=255)
                                    tAATGCCCGCCAGCGCCAGAGCGACGTGTTTGGGTTGCAGTGCCGGCATGATCCCACTGAAATCCAGCGGCGGCCGTTCGTCAGCCCGCTGCAGCTCTTTAGCGATGGCCGCACCCCGATCAACGTCACAGCCCCCATCGGGATCGCCCt                                                                                                               >  2:2147738/1‑150 (MQ=255)
                                     aaTGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTTCAGTGCCGGAATTATCCCACTGAAATACATCGGCTTCAGTTCGTAATACAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAAAGTCCAAGCCCACATATTTATCGcccag                                                                                                              >  1:503419/1‑148 (MQ=255)
                                           cgccagcgccagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTaaa                                                                                                        <  1:2626808/150‑1 (MQ=255)
                                             ccagcgccagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAATCCCACATATTTATCGCCCTGTTTAAAtt                                                                                                      >  2:3028324/1‑150 (MQ=255)
                                             ccagcgccagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAAtt                                                                                                      >  1:3468825/1‑150 (MQ=255)
                                              cagcgccagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTc                                                                                                     >  2:4617612/1‑150 (MQ=255)
                                                gcgccagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCaa                                                                                                   <  1:1387982/150‑1 (MQ=255)
                                                    cagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAAAgg                                                                                               >  2:3438183/1‑150 (MQ=255)
                                                      gATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGaa                                                                                             <  2:1764738/150‑1 (MQ=255)
                                                         cGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGa                                                                                          >  2:3875878/1‑150 (MQ=255)
                                                         cGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACAGAAATCCATCGGCTTCAGTTCGTAATACAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACCTCAAAGCCCACATATTTATAGCCCTGTTTAAATTCAAACGGAACGa                                                                                          >  1:2484320/1‑150 (MQ=255)
                                                          gACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGaa                                                                                         >  1:1100216/1‑150 (MQ=255)
                                                          gACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGaa                                                                                         >  1:2942698/1‑150 (MQ=255)
                                                             ttttttGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTTTTTAAATTCAAACGGAACGAAGGc                                                                                      <  1:3183284/149‑1 (MQ=255)
                                                              tttttGGTTTGCAGTGCCGGAATGATCCCACTTAAATCAATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCg                                                                                     <  1:4392644/150‑1 (MQ=255)
                                                              tttttGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCg                                                                                     <  1:2113419/150‑1 (MQ=255)
                                                               ttttGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCgg                                                                                    >  1:1875889/1‑150 (MQ=255)
                                                                 ttGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTa                                                                                  <  1:4072499/150‑1 (MQ=255)
                                                                 ttGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGATTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTa                                                                                  <  1:3234754/150‑1 (MQ=255)
                                                                   ggTGTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATc                                                                                <  2:2859538/150‑1 (MQ=255)
                                                                    gTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATcc                                                                               <  1:4631242/150‑1 (MQ=255)
                                                                       tGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTc                                                                            <  2:1221821/150‑1 (MQ=255)
                                                                       tGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTc                                                                            <  1:1777553/150‑1 (MQ=255)
                                                                          aGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTcgcg                                                                         <  2:4144463/150‑1 (MQ=255)
                                                                                 gAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACACCTa                                                                  >  2:3823624/1‑150 (MQ=255)
                                                                                 gAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTa                                                                  >  2:400038/1‑150 (MQ=255)
                                                                                   aTGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAt                                                                <  2:1747511/150‑1 (MQ=255)
                                                                                   aTGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAt                                                                <  1:3143364/150‑1 (MQ=255)
                                                                                    tGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAtt                                                               >  1:893373/1‑150 (MQ=255)
                                                                                    tGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAtt                                                               <  1:3000628/150‑1 (MQ=255)
                                                                                     gATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAttt                                                              <  1:3664392/150‑1 (MQ=255)
                                                                                     gATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGACCGAAGGCGGTCTCCGTCGCGACACCTAAttt                                                              >  1:4089190/1‑150 (MQ=255)
                                                                                     gATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAttt                                                              >  1:1150021/1‑150 (MQ=255)
                                                                                      aTCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAtttt                                                             <  1:3810089/150‑1 (MQ=255)
                                                                                        cccACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAtttttt                                                           >  2:2938042/1‑150 (MQ=255)
                                                                                               aaaTCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCcgcg                                                    <  2:3704198/150‑1 (MQ=255)
                                                                                                 aTCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGc                                                  <  1:3833613/150‑1 (MQ=255)
                                                                                                        ggCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGagaa                                           >  1:192384/1‑150 (MQ=255)
                                                                                                          cTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGagaaga                                         <  1:2336532/150‑1 (MQ=255)
                                                                                                          cTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGagaaga                                         <  2:2055656/150‑1 (MQ=255)
                                                                                                           ttCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAgaagaa                                        >  2:1836580/1‑150 (MQ=255)
                                                                                                           ttCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAgaagaa                                        <  1:2097619/150‑1 (MQ=255)
                                                                                                           ttCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAgaagaa                                        <  1:1981905/150‑1 (MQ=255)
                                                                                                             cAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAAc                                      <  2:3079644/150‑1 (MQ=255)
                                                                                                              aggtCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAAcc                                     >  2:3642116/4‑150 (MQ=255)
                                                                                                              aGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAAcc                                     >  2:4525626/1‑150 (MQ=255)
                                                                                                              aGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAAcc                                     <  2:1598352/150‑1 (MQ=255)
                                                                                                              aGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAAcc                                     <  2:2145329/150‑1 (MQ=255)
                                                                                                                 tCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCa                                  >  2:2416643/1‑150 (MQ=255)
                                                                                                                 tCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCa                                  <  2:752582/150‑1 (MQ=255)
                                                                                                                 tCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCa                                  <  2:3120497/150‑1 (MQ=255)
                                                                                                                     aaTCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAg                              <  2:3347690/150‑1 (MQ=255)
                                                                                                                       tCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGcc                            <  1:4006070/150‑1 (MQ=255)
                                                                                                                         cAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAg                          <  1:244181/150‑1 (MQ=255)
                                                                                                                         cAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAg                          <  1:244434/150‑1 (MQ=255)
                                                                                                                          aGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAgg                         <  1:4742692/150‑1 (MQ=255)
                                                                                                                               cAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAg                    >  2:1332733/1‑150 (MQ=255)
                                                                                                                                    ctTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAg               <  1:275419/150‑1 (MQ=255)
                                                                                                                                      ttAGCGATGGCAGCCCACAGACCAACGACAAAGCCCACATATTGATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGcc             <  2:3242733/150‑1 (MQ=255)
                                                                                                                                        aGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAg           >  2:199770/1‑150 (MQ=255)
                                                                                                                                        aGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAg           >  1:1126489/1‑150 (MQ=255)
                                                                                                                                          cGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTg         <  1:3446893/150‑1 (MQ=255)
                                                                                                                                          cGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTg         <  2:192037/150‑1 (MQ=255)
                                                                                                                                           gATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGa        >  1:3652459/1‑150 (MQ=255)
                                                                                                                                           gATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGa        >  1:1256769/1‑150 (MQ=255)
                                                                                                                                           gATGGCAGCACACAGATCAACGTCAAAGCCCACATATGTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGa        <  2:1907192/150‑1 (MQ=255)
                                                                                                                                            aTGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGaa       <  1:2852594/150‑1 (MQ=255)
                                                                                                                                               gcagcaCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACt    <  1:1913149/150‑1 (MQ=255)
                                                                                                                                                 aGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACttt  >  2:4311522/1‑150 (MQ=255)
                                                                                                                                                 aGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACttt  <  2:595678/150‑1 (MQ=255)
                                                                                                                                                 aGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATACGCGGCATGAGAAGAAACCGCAAAAGCCACGGTCAGTGCAGCCAGTGAAACttt  >  2:2942615/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AGAAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTT  >  CP009273/843151‑843445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A