Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 843,299 C→A 100% W54C (TGG→TGT)  glnH ← glutamine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273843,2990CA96.4% 742.7 / 15.5 223W54C (TGG→TGT) glnHglutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (4/4);  new base A (103/112);  total (107/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 230 total aligned reads displayed.

AGAAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTT  >  CP009273/843151‑843445
                                                                                                                                                    |                                                                                                                                                  
agaAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGccc                                                                                                                                                   <  2:3256461/150‑1 (MQ=255)
 gaAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCca                                                                                                                                                  <  1:565174/150‑1 (MQ=255)
  aaaTCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCacac                                                                                                                                                 >  2:3382372/1‑150 (MQ=255)
         tcgCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCaa                                                                                                                                          <  1:1023892/150‑1 (MQ=255)
          cgCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAAc                                                                                                                                         <  1:134479/150‑1 (MQ=255)
          cgCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAAc                                                                                                                                         >  1:2993043/1‑150 (MQ=255)
           gCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACg                                                                                                                                        <  1:1322063/150‑1 (MQ=255)
            cTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGt                                                                                                                                       >  1:2360033/1‑150 (MQ=255)
             ttttttACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTc                                                                                                                                      >  1:239142/1‑150 (MQ=255)
             ttttttACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTc                                                                                                                                      >  1:2627170/1‑150 (MQ=255)
              tttttACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCa                                                                                                                                     <  1:4068645/150‑1 (MQ=255)
               ttttACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCaa                                                                                                                                    <  1:441241/150‑1 (MQ=255)
                 ttACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAg                                                                                                                                  >  1:114560/1‑150 (MQ=255)
                   aCGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGcc                                                                                                                                >  2:3963544/1‑150 (MQ=255)
                      cTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCcac                                                                                                                             >  1:3969273/1‑150 (MQ=255)
                      cTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCcac                                                                                                                             >  1:3927686/1‑150 (MQ=255)
                        cgtcgGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACat                                                                                                                           >  2:377917/1‑150 (MQ=255)
                        cgtcgGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACat                                                                                                                           <  2:3783343/150‑1 (MQ=255)
                           cgGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATAtt                                                                                                                        <  1:976295/150‑1 (MQ=255)
                            ggTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATAttt                                                                                                                       <  1:3464113/150‑1 (MQ=255)
                            ggTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATAttt                                                                                                                       <  1:3269331/150‑1 (MQ=255)
                            ggTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATAttt                                                                                                                       >  2:3341370/1‑150 (MQ=255)
                               gATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATc                                                                                                                    >  2:417388/1‑150 (MQ=255)
                                     aaTGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTg                                                                                                              <  2:1604039/150‑1 (MQ=255)
                                     aaTGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTg                                                                                                              >  2:2214476/1‑150 (MQ=255)
                                       tGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGtt                                                                                                            <  1:3744881/150‑1 (MQ=255)
                                        gCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGttt                                                                                                           <  1:1992093/150‑1 (MQ=255)
                                        gCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGttt                                                                                                           <  2:3835192/150‑1 (MQ=255)
                                         cccGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTa                                                                                                          <  2:2856861/150‑1 (MQ=255)
                                          ccGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTaa                                                                                                         <  2:1027109/150‑1 (MQ=255)
                                           cgccagcgccagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTaaa                                                                                                        <  1:2600467/150‑1 (MQ=255)
                                             ccagcgccagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGTACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAAtt                                                                                                      <  2:3664644/150‑1 (MQ=255)
                                                   ccagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACg                                                                                                <  2:828171/150‑1 (MQ=255)
                                                   ccagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTCCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACg                                                                                                <  2:1275848/150‑1 (MQ=255)
                                                     agATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGa                                                                                              >  1:3188606/1‑150 (MQ=255)
                                                     agATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGa                                                                                              <  1:1212305/150‑1 (MQ=255)
                                                       aTCGACGTTTTTGGTTTTCAGTGCCGGAATGATCCCACTGAAATACAACGGCTTCAGTTCGTAAACCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAAAGTCAAATCCCACAAATTTAACTCCCAGATTAAATTAAAACGGAAc                                                                                            >  2:1091335/1‑150 (MQ=255)
                                                         cGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGa                                                                                          >  2:557954/1‑150 (MQ=255)
                                                          gACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGaa                                                                                         >  1:2560114/1‑150 (MQ=255)
                                                           aCGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAg                                                                                        <  2:2534972/150‑1 (MQ=255)
                                                            cGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAgg                                                                                       <  1:3581098/150‑1 (MQ=255)
                                                            cGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAgg                                                                                       >  1:1498342/1‑150 (MQ=255)
                                                            cGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAgg                                                                                       >  1:4158527/1‑150 (MQ=255)
                                                                  tGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATCTTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTAt                                                                                 <  1:1819277/150‑1 (MQ=255)
                                                                    gTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATcc                                                                               <  1:44694/150‑1 (MQ=255)
                                                                     tttGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCg                                                                              >  1:608024/1‑150 (MQ=255)
                                                                      ttGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGt                                                                             <  1:3722611/150‑1 (MQ=255)
                                                                      ttGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGt                                                                             >  2:3072004/1‑150 (MQ=255)
                                                                       tGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTc                                                                            <  1:643006/150‑1 (MQ=255)
                                                                        gCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGCAGGCGGTATCCGTcg                                                                           >  2:515846/1‑150 (MQ=255)
                                                                         cAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTAGCCGTcgc                                                                          >  1:2810066/1‑150 (MQ=255)
                                                                             gCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACa                                                                      <  2:181078/150‑1 (MQ=255)
                                                                                 gAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTa                                                                  >  1:924075/1‑150 (MQ=255)
                                                                                 gAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTa                                                                  >  1:924104/1‑150 (MQ=255)
                                                                                  aaTGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTaa                                                                 >  2:3574310/1‑150 (MQ=255)
                                                                                   aTGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAt                                                                <  2:2957675/150‑1 (MQ=255)
                                                                                     gATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAttt                                                              >  2:3958670/1‑150 (MQ=255)
                                                                                      aTCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGACCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAtttt                                                             >  2:4005895/1‑150 (MQ=255)
                                                                                      aTCCCACTGAAATCCATCGGATTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAtttt                                                             <  2:346427/150‑1 (MQ=255)
                                                                                       tCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAttttt                                                            <  1:2431678/150‑1 (MQ=255)
                                                                                       tCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAttttt                                                            <  2:236543/150‑1 (MQ=255)
                                                                                         ccACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTa                                                          <  2:2899873/150‑1 (MQ=255)
                                                                                             tGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCcg                                                      <  2:1519300/150‑1 (MQ=255)
                                                                                               aaaTCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCcgcg                                                    >  1:1735578/1‑150 (MQ=255)
                                                                                               aaaTCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCcgcg                                                    >  1:299575/1‑150 (MQ=255)
                                                                                                     aTCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATga                                              <  2:3277883/150‑1 (MQ=255)
                                                                                                            tCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGaaa                                       <  1:3369245/150‑1 (MQ=255)
                                                                                                             cAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAAc                                      >  1:3132664/1‑150 (MQ=255)
                                                                                                                 tCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCa                                  <  2:3414932/150‑1 (MQ=255)
                                                                                                                  cGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCaa                                 >  1:1435996/1‑150 (MQ=255)
                                                                                                                  cGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACAAATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCaa                                 >  1:344973/1‑150 (MQ=255)
                                                                                                                    tAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCaaaa                               <  2:2814542/150‑1 (MQ=255)
                                                                                                                    tAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCaaaa                               <  2:2480294/150‑1 (MQ=255)
                                                                                                                        ccAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCa                           <  2:775305/150‑1 (MQ=255)
                                                                                                                          aGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAgg                         >  1:742406/1‑150 (MQ=255)
                                                                                                                          aGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAgg                         <  1:2042958/150‑1 (MQ=255)
                                                                                                                          aGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAgg                         >  2:728791/1‑150 (MQ=255)
                                                                                                                             ttCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTc                      >  1:2858666/1‑150 (MQ=255)
                                                                                                                                aGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGt                   >  2:3138875/1‑150 (MQ=255)
                                                                                                                                 gCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTg                  <  2:141456/150‑1 (MQ=255)
                                                                                                                                 gCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTg                  <  2:1096358/150‑1 (MQ=255)
                                                                                                                                  ctctTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGc                 <  2:449707/150‑1 (MQ=255)
                                                                                                                                   tctTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCa                >  1:2261602/1‑150 (MQ=255)
                                                                                                                                    ctTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTCTCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAg               >  1:2693996/1‑150 (MQ=255)
                                                                                                                                    ctTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAg               <  2:1936354/150‑1 (MQ=255)
                                                                                                                                    ctTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAg               <  2:2821040/150‑1 (MQ=255)
                                                                                                                                       tAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCa            <  2:2956901/150‑1 (MQ=255)
                                                                                                                                        aGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAg           >  1:176203/1‑150 (MQ=255)
                                                                                                                                        aGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAg           >  1:2341011/1‑150 (MQ=255)
                                                                                                                                        aGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAg           >  1:1542086/1‑150 (MQ=255)
                                                                                                                                        aGCGATGGCAGCACAAAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAg           >  1:178451/1‑150 (MQ=255)
                                                                                                                                          cGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTg         <  1:1243957/150‑1 (MQ=255)
                                                                                                                                           gATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGACGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGa        <  1:1160168/150‑1 (MQ=255)
                                                                                                                                             tGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGaaa      <  2:2756953/150‑1 (MQ=255)
                                                                                                                                              ggCAGCACACAGATCAACGTCAAATCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAAc     >  1:1930488/1‑150 (MQ=255)
                                                                                                                                                cAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACtt   >  2:3607664/1‑150 (MQ=255)
                                                                                                                                                cAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACtt   <  1:314980/150‑1 (MQ=255)
                                                                                                                                                 aGCACACAGATCAACGTCAAAGCCCACATATTTCTCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACttt  <  2:1339608/150‑1 (MQ=255)
                                                                                                                                                 aGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACttt  <  2:3433284/150‑1 (MQ=255)
                                                                                                                                                 aGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACttt  <  2:3092250/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AGAAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTT  >  CP009273/843151‑843445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGAAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTTTAA  >  CP009273/843151‑843448
                                                                                                                                                    |                                                                                                                                                     
AGAAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCAC                                                                                                                                                      >  SRR19973074.2304711/1‑150 (MQ=60)
 GAAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCA                                                                                                                                                     <  SRR19973074.566286/150‑1 (MQ=60)
   AATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACA                                                                                                                                                   <  SRR19973074.2943126/150‑1 (MQ=60)
   AATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACA                                                                                                                                                   <  SRR19973074.446292/150‑1 (MQ=60)
        ATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCA                                                                                                                                              >  SRR19973074.2234329/1‑150 (MQ=60)
         TCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAA                                                                                                                                             <  SRR19973074.1025787/150‑1 (MQ=60)
          CGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAAC                                                                                                                                            >  SRR19973074.2998605/1‑150 (MQ=60)
          CGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAAC                                                                                                                                            <  SRR19973074.3936979/150‑1 (MQ=60)
          CGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAAC                                                                                                                                            <  SRR19973074.134793/150‑1 (MQ=60)
          CGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAAC                                                                                                                                            <  SRR19973074.134772/150‑1 (MQ=60)
           GCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACG                                                                                                                                           >  SRR19973074.3222813/1‑150 (MQ=60)
           GCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACG                                                                                                                                           <  SRR19973074.74210/150‑1 (MQ=60)
           GCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACG                                                                                                                                           <  SRR19973074.1324542/150‑1 (MQ=60)
            CTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGT                                                                                                                                          >  SRR19973074.2364512/1‑150 (MQ=60)
            CTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGT                                                                                                                                          >  SRR19973074.2364534/1‑150 (MQ=60)
            CTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGT                                                                                                                                          >  SRR19973074.2364426/1‑150 (MQ=60)
             TTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTC                                                                                                                                         >  SRR19973074.239626/1‑150 (MQ=60)
             TTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTC                                                                                                                                         >  SRR19973074.2632083/1‑150 (MQ=60)
              TTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCA                                                                                                                                        <  SRR19973074.4076174/150‑1 (MQ=60)
               TTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAA                                                                                                                                       <  SRR19973074.442095/150‑1 (MQ=60)
               TTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAA                                                                                                                                       <  SRR19973074.442081/150‑1 (MQ=60)
               TTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAA                                                                                                                                       <  SRR19973074.3076012/150‑1 (MQ=60)
                 TTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAG                                                                                                                                     >  SRR19973074.1359660/1‑150 (MQ=60)
                 TTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAG                                                                                                                                     >  SRR19973074.114834/1‑150 (MQ=60)
                  TACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGC                                                                                                                                    <  SRR19973074.3295022/150‑1 (MQ=60)
                   ACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCC                                                                                                                                   >  SRR19973074.637018/1‑150 (MQ=60)
                      CTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCAC                                                                                                                                >  SRR19973074.3934912/1‑150 (MQ=60)
                      CTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCAC                                                                                                                                <  SRR19973074.3437566/150‑1 (MQ=60)
                      CTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCAC                                                                                                                                >  SRR19973074.3976592/1‑150 (MQ=60)
                           CGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATT                                                                                                                           <  SRR19973074.978129/150‑1 (MQ=60)
                            GGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTT                                                                                                                          <  SRR19973074.3275389/150‑1 (MQ=60)
                            GGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTT                                                                                                                          <  SRR19973074.3470480/150‑1 (MQ=60)
                              TGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTAT                                                                                                                        >  SRR19973074.1822581/1‑150 (MQ=60)
                               GATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATC                                                                                                                       >  SRR19973074.1412284/1‑150 (MQ=60)
                                    TAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCT                                                                                                                  >  SRR19973074.577062/1‑150 (MQ=60)
                                    TAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCT                                                                                                                  >  SRR19973074.2705900/1‑150 (MQ=60)
                                       TGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTT                                                                                                               >  SRR19973074.2849216/1‑150 (MQ=60)
                                       TGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTT                                                                                                               <  SRR19973074.3751818/150‑1 (MQ=60)
                                       TGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTT                                                                                                               <  SRR19973074.3751774/150‑1 (MQ=60)
                                        GCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTT                                                                                                              <  SRR19973074.1995874/150‑1 (MQ=60)
                                          CCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAA                                                                                                            <  SRR19973074.1640815/150‑1 (MQ=60)
                                           CGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGTATGATCCCACTGTAATCCATCGGCTTCAGTTCGTAATACAGCTTAAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAA                                                                                                           <  SRR19973074.2604936/150‑1 (MQ=60)
                                           CGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAA                                                                                                           <  SRR19973074.2605333/150‑1 (MQ=60)
                                              CAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTC                                                                                                        >  SRR19973074.3592509/1‑150 (MQ=60)
                                                     AGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGA                                                                                                 <  SRR19973074.1214631/150‑1 (MQ=60)
                                                     AGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGA                                                                                                 >  SRR19973074.3194480/1‑150 (MQ=60)
                                                      GATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAA                                                                                                <  SRR19973074.3179011/150‑1 (MQ=60)
                                                       ATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAAC                                                                                               <  SRR19973074.2636796/150‑1 (MQ=60)
                                                         CGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGA                                                                                             >  SRR19973074.911347/1‑150 (MQ=60)
                                                          GACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAA                                                                                            >  SRR19973074.2564893/1‑150 (MQ=60)
                                                            CGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGG                                                                                          >  SRR19973074.4135910/1‑150 (MQ=60)
                                                            CGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGG                                                                                          >  SRR19973074.1501166/1‑150 (MQ=60)
                                                            CGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGG                                                                                          >  SRR19973074.1501172/1‑150 (MQ=60)
                                                            CGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGG                                                                                          <  SRR19973074.3587702/150‑1 (MQ=60)
                                                            CGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGG                                                                                          >  SRR19973074.4166179/1‑150 (MQ=60)
                                                               TTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGG                                                                                       <  SRR19973074.1908823/150‑1 (MQ=60)
                                                                  TGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATCTTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTAT                                                                                    <  SRR19973074.1822743/150‑1 (MQ=60)
                                                                  TGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTAT                                                                                    <  SRR19973074.1822814/150‑1 (MQ=60)
                                                                    GTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCC                                                                                  <  SRR19973074.2617149/150‑1 (MQ=60)
                                                                    GTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCC                                                                                  <  SRR19973074.44822/150‑1 (MQ=60)
                                                                     TTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCG                                                                                 >  SRR19973074.609179/1‑150 (MQ=60)
                                                                     TTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCG                                                                                 >  SRR19973074.1960013/1‑150 (MQ=60)
                                                                     TTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCG                                                                                 <  SRR19973074.1168721/150‑1 (MQ=60)
                                                                     TTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTAGCCG                                                                                 >  SRR19973074.609033/1‑150 (MQ=60)
                                                                      TTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGT                                                                                <  SRR19973074.3729659/150‑1 (MQ=60)
                                                                      TTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGT                                                                                <  SRR19973074.3729475/150‑1 (MQ=60)
                                                                       TGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTC                                                                               <  SRR19973074.644215/150‑1 (MQ=60)
                                                                         CAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTAGCCGTCGC                                                                             >  SRR19973074.2815255/1‑150 (MQ=60)
                                                                                GGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACT                                                                      >  SRR19973074.2401379/1‑150 (MQ=60)
                                                                                 GAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTA                                                                     >  SRR19973074.925834/1‑150 (MQ=60)
                                                                                 GAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTA                                                                     >  SRR19973074.925838/1‑150 (MQ=60)
                                                                                 GAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTA                                                                     >  SRR19973074.925863/1‑150 (MQ=60)
                                                                                   ATGAACCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCATTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAT                                                                   >  SRR19973074.346420/1‑150 (MQ=60)
                                                                                       TCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTT                                                               <  SRR19973074.2436174/150‑1 (MQ=60)
                                                                                           ACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATC                                                           <  SRR19973074.3920601/150‑1 (MQ=60)
                                                                                              GAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGC                                                        >  SRR19973074.1235510/1‑150 (MQ=60)
                                                                                               AAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAATCGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCG                                                       >  SRR19973074.299949/1‑150 (MQ=60)
                                                                                               AAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCG                                                       >  SRR19973074.300132/1‑150 (MQ=60)
                                                                                               AAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCG                                                       >  SRR19973074.1738788/1‑150 (MQ=60)
                                                                                               AAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCG                                                       >  SRR19973074.200162/1‑150 (MQ=60)
                                                                                                   CCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCAT                                                   <  SRR19973074.3138093/150‑1 (MQ=60)
                                                                                                   CCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCAT                                                   <  SRR19973074.945845/150‑1 (MQ=60)
                                                                                                       CGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGA                                               <  SRR19973074.765500/150‑1 (MQ=60)
                                                                                                        GGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAA                                              <  SRR19973074.2358119/150‑1 (MQ=60)
                                                                                                         GCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAG                                             <  SRR19973074.2609322/150‑1 (MQ=60)
                                                                                                            TCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAA                                          <  SRR19973074.3375491/150‑1 (MQ=60)
                                                                                                             CAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAAC                                         >  SRR19973074.3138674/1‑150 (MQ=60)
                                                                                                             CAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAAC                                         >  SRR19973074.3138403/1‑150 (MQ=60)
                                                                                                              AGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACC                                        <  SRR19973074.2818060/150‑1 (MQ=60)
                                                                                                              AGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACC                                        <  SRR19973074.1598687/150‑1 (MQ=60)
                                                                                                                  CGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAA                                    >  SRR19973074.3559383/1‑150 (MQ=60)
                                                                                                                  CGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAA                                    >  SRR19973074.1438671/1‑150 (MQ=60)
                                                                                                                  CGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAA                                    >  SRR19973074.1760947/1‑150 (MQ=60)
                                                                                                                  CGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCAGGAGAAGAAACCGCAA                                    >  SRR19973074.3559385/1‑150 (MQ=60)
                                                                                                                  CGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACAAATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAA                                    >  SRR19973074.345597/1‑150 (MQ=60)
                                                                                                                    TAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAA                                  >  SRR19973074.1232132/1‑150 (MQ=60)
                                                                                                                      ATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGC                                >  SRR19973074.1091220/1‑150 (MQ=60)
                                                                                                                       TCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCC                               >  SRR19973074.2458081/1‑150 (MQ=60)
                                                                                                                          AGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGG                            >  SRR19973074.743800/1‑150 (MQ=60)
                                                                                                                          AGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGG                            <  SRR19973074.2046806/150‑1 (MQ=60)
                                                                                                                            CTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGT                          >  SRR19973074.2225362/1‑150 (MQ=60)
                                                                                                                             TTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTC                         >  SRR19973074.1278879/1‑150 (MQ=60)
                                                                                                                             TTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTC                         >  SRR19973074.2863970/1‑150 (MQ=60)
                                                                                                                              TCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCA                        <  SRR19973074.4016877/150‑1 (MQ=60)
                                                                                                                                   TCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCA                   >  SRR19973074.2265847/1‑150 (MQ=60)
                                                                                                                                    CTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTCTCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAG                  >  SRR19973074.2699000/1‑150 (MQ=60)
                                                                                                                                       TAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCA               <  SRR19973074.3366837/150‑1 (MQ=60)
                                                                                                                                       TAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCA               >  SRR19973074.1518020/1‑150 (MQ=60)
                                                                                                                                       TAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCA               <  SRR19973074.432542/150‑1 (MQ=60)
                                                                                                                                        AGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAG              >  SRR19973074.2345388/1‑150 (MQ=60)
                                                                                                                                        AGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAG              >  SRR19973074.176598/1‑150 (MQ=60)
                                                                                                                                        AGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAG              >  SRR19973074.176584/1‑150 (MQ=60)
                                                                                                                                        AGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAG              >  SRR19973074.1545010/1‑150 (MQ=60)
                                                                                                                                        AGCGATGGCAGCACAAAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAG              >  SRR19973074.178837/1‑150 (MQ=60)
                                                                                                                                          CGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTG            <  SRR19973074.1246341/150‑1 (MQ=60)
                                                                                                                                           GATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGACGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGA           <  SRR19973074.1162376/150‑1 (MQ=60)
                                                                                                                                           GATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGA           <  SRR19973074.1162350/150‑1 (MQ=60)
                                                                                                                                              GGCAGCACACAGATCAACGTCAAATCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAAC        >  SRR19973074.1934179/1‑150 (MQ=60)
                                                                                                                                              GGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAAC        >  SRR19973074.47120/1‑150 (MQ=60)
                                                                                                                                                CAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTT      <  SRR19973074.315558/150‑1 (MQ=60)
                                                                                                                                                 AGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTT     >  SRR19973074.2311643/1‑150 (MQ=60)
                                                                                                                                                  GCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTTT    <  SRR19973074.1995411/150‑1 (MQ=60)
                                                                                                                                                   CACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTAACCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTTTA   >  SRR19973074.461847/1‑150 (MQ=60)
                                                                                                                                                    ACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGCCGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTTTAA  <  SRR19973074.378628/150‑1 (MQ=60)
                                                                                                                                                    ACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTTTAA  <  SRR19973074.378819/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
AGAAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTTTAA  >  CP009273/843151‑843448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑