Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 843,299 C→A 87.5% W54C (TGG→TGT)  glnH ← glutamine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273843,2990CA87.5% 726.5 / 101.9 273W54C (TGG→TGT) glnHglutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (18/16);  new base A (119/119);  total (137/136)
Fisher's exact test for biased strand distribution p-value = 8.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

Only 100 of 275 total aligned reads displayed.

AGAAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTT  >  CP009273/843151‑843445
                                                                                                                                                    |                                                                                                                                                  
       gatcgCTTTTTTACGCTCGTCGGTGATTGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATc                                                                                                                                            >  2:4141597/1‑150 (MQ=255)
       gatcgCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATc                                                                                                                                            >  1:3631473/1‑150 (MQ=255)
       gatcgCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATc                                                                                                                                            <  2:3925834/150‑1 (MQ=255)
       gatcgCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATc                                                                                                                                            >  2:3893390/1‑150 (MQ=255)
       gatcgCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATc                                                                                                                                            >  1:704690/1‑150 (MQ=255)
        atcgCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCa                                                                                                                                           <  1:4881131/150‑1 (MQ=255)
          cgCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAAc                                                                                                                                         <  2:3502197/150‑1 (MQ=255)
           gCTTTTTTCCGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACg                                                                                                                                        <  2:3669177/150‑1 (MQ=255)
           gCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACg                                                                                                                                        <  2:3669471/150‑1 (MQ=255)
                 ttACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAg                                                                                                                                  >  1:4341596/1‑150 (MQ=255)
                      cTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCcac                                                                                                                             >  1:852098/1‑150 (MQ=255)
                          tcgGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACatat                                                                                                                         >  2:5051513/1‑150 (MQ=255)
                           cgGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATAtt                                                                                                                        >  1:2819341/1‑150 (MQ=255)
                           cgGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATAtt                                                                                                                        <  2:1618497/150‑1 (MQ=255)
                           cgGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATAtt                                                                                                                        <  1:449437/150‑1 (MQ=255)
                            ggTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATAttt                                                                                                                       >  1:630122/1‑150 (MQ=255)
                             gTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTa                                                                                                                      >  2:2704843/1‑150 (MQ=255)
                              tGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATTATCCCACTGAAATCCATCGGCTTCAGTTAGGAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAAAGTCAAATCCCACAAATTTAt                                                                                                                     >  1:891611/1‑150 (MQ=255)
                               gATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATc                                                                                                                    <  2:4699452/150‑1 (MQ=255)
                               gATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATc                                                                                                                    <  2:929932/150‑1 (MQ=255)
                                aTGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCg                                                                                                                   >  2:4912271/1‑150 (MQ=255)
                                  ggTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGcc                                                                                                                 <  2:4301081/150‑1 (MQ=255)
                                       tGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGtt                                                                                                            <  2:2575475/150‑1 (MQ=255)
                                        gCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGttt                                                                                                           >  2:4023430/1‑150 (MQ=255)
                                        gCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGttt                                                                                                           <  2:40866/150‑1 (MQ=255)
                                          ccGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTaa                                                                                                         <  2:2170719/150‑1 (MQ=255)
                                          ccGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTaa                                                                                                         <  2:4231524/150‑1 (MQ=255)
                                            gccagcgccagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAAt                                                                                                       >  2:4008727/1‑150 (MQ=255)
                                              cagcgccagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTc                                                                                                     <  2:2170073/150‑1 (MQ=255)
                                                gcgccagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCaa                                                                                                   >  2:1055316/1‑150 (MQ=255)
                                                   ccagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACg                                                                                                >  2:794113/1‑150 (MQ=255)
                                                   ccagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACg                                                                                                >  2:794138/1‑150 (MQ=255)
                                                    cagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACgg                                                                                               >  1:1606982/1‑150 (MQ=255)
                                                    cagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACgg                                                                                               >  2:2958812/1‑150 (MQ=255)
                                                    cagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACgg                                                                                               >  2:1178367/1‑150 (MQ=255)
                                                     agATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGa                                                                                              >  1:5130926/1‑150 (MQ=255)
                                                       aTCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAAc                                                                                            >  2:709480/1‑150 (MQ=255)
                                                          gACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGaa                                                                                         >  2:836632/1‑150 (MQ=255)
                                                            cGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAgg                                                                                       >  2:3079363/1‑150 (MQ=255)
                                                            cGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAgg                                                                                       >  2:2596513/1‑150 (MQ=255)
                                                            cGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAgg                                                                                       <  1:3456046/150‑1 (MQ=255)
                                                             gTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGc                                                                                      >  1:26192/1‑150 (MQ=255)
                                                                tttGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGt                                                                                   <  1:4375930/150‑1 (MQ=255)
                                                                   ggTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACG‑AGGCGGTATcc                                                                               >  1:1038143/1‑150 (MQ=255)
                                                                    gTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATcc                                                                               >  2:3165832/1‑150 (MQ=255)
                                                                    gTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATcc                                                                               >  2:2515048/1‑150 (MQ=255)
                                                                     tttGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCg                                                                              >  1:2241520/1‑150 (MQ=255)
                                                                       tGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTc                                                                            <  2:2276232/150‑1 (MQ=255)
                                                                        gCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTcg                                                                           <  2:2772070/150‑1 (MQ=255)
                                                                          aGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTcgcg                                                                         >  1:3889800/1‑150 (MQ=255)
                                                                              ccGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACaa                                                                     <  1:4018872/150‑1 (MQ=255)
                                                                               cGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAAc                                                                    <  2:1128440/150‑1 (MQ=255)
                                                                               cGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAAc                                                                    >  1:2496385/1‑150 (MQ=255)
                                                                               cGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAAc                                                                    <  2:4335296/150‑1 (MQ=255)
                                                                                ggAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACt                                                                   <  1:979044/150‑1 (MQ=255)
                                                                                 gAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTa                                                                  <  2:3598541/150‑1 (MQ=255)
                                                                                   aTGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAt                                                                >  2:1569009/1‑150 (MQ=255)
                                                                                      aTCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAtttt                                                             <  1:3058229/150‑1 (MQ=255)
                                                                                      aTCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAtttt                                                             <  2:3719489/150‑1 (MQ=255)
                                                                                       tCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTCTCCGTCGCGACAACTAAttttt                                                            >  2:5062282/1‑150 (MQ=255)
                                                                                         ccACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTa                                                          >  1:2317343/1‑150 (MQ=255)
                                                                                          cACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTAt                                                         <  2:3487486/150‑1 (MQ=255)
                                                                                           aCTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATc                                                        <  1:371836/150‑1 (MQ=255)
                                                                                            cTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATcc                                                       <  1:3215468/150‑1 (MQ=255)
                                                                                            cTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAAAGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATACGTCGCGACAACTAATTTTTTATcc                                                       <  1:3134523/150‑1 (MQ=255)
                                                                                             tGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCcg                                                      >  1:1497368/1‑150 (MQ=255)
                                                                                             tGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCcg                                                      >  1:4918895/1‑150 (MQ=255)
                                                                                              gAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCcgc                                                     <  2:3424300/150‑1 (MQ=255)
                                                                                               aaaTCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCcgcg                                                    <  2:3139226/150‑1 (MQ=255)
                                                                                               aaaTCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCcgcg                                                    >  1:3980419/1‑150 (MQ=255)
                                                                                                 aTCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGc                                                  <  1:3373488/150‑1 (MQ=255)
                                                                                                 aTCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGc                                                  >  1:9174/1‑150 (MQ=255)
                                                                                                 aTCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGc                                                  >  1:1739684/1‑150 (MQ=255)
                                                                                                  tCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCa                                                 >  1:5075779/1‑150 (MQ=255)
                                                                                                   ccATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCAt                                                <  1:3235980/150‑1 (MQ=255)
                                                                                                    cATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATg                                               >  2:3812630/1‑150 (MQ=255)
                                                                                                        ggCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGagaa                                           >  1:1384026/1‑150 (MQ=255)
                                                                                                        ggCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGagaa                                           <  2:1401160/150‑1 (MQ=255)
                                                                                                              aGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAAcc                                     >  2:1578559/1‑150 (MQ=255)
                                                                                                                  cGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCaa                                 >  2:2728520/1‑150 (MQ=255)
                                                                                                                  cGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCaa                                 <  1:124656/150‑1 (MQ=255)
                                                                                                                    tAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAATCCCTCATATTTATCGCCCTGATTATATTCAATCGTAACGAAGGCGGTATCCGTCGCTACAACTAATTTTTTATCCGCTGCATGAGAAGAAACCGCaaat                               <  2:1338549/150‑2 (MQ=255)
                                                                                                                    tAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCaaaa                               >  2:3951058/1‑150 (MQ=255)
                                                                                                                    tAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCAAATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCTGTATCCGTATCGACATCTAATTTTTTATCCGCGGCATGAGAAGAAACCGCaaaa                               <  2:4651148/150‑1 (MQ=255)
                                                                                                                     aaTCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAg                              >  2:1827524/1‑150 (MQ=255)
                                                                                                                     aaTCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAg                              >  1:4192103/1‑150 (MQ=255)
                                                                                                                        ccAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCa                           >  2:245062/1‑150 (MQ=255)
                                                                                                                           gCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAggg                        <  2:2317343/150‑1 (MQ=255)
                                                                                                                            cTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGt                       <  1:2309550/150‑1 (MQ=255)
                                                                                                                              tCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCa                     <  1:5062149/150‑1 (MQ=255)
                                                                                                                                  ctctTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGc                 >  1:1389030/1‑150 (MQ=255)
                                                                                                                                    ctTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAg               >  2:4465212/1‑150 (MQ=255)
                                                                                                                                    ctTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAg               >  2:452327/1‑150 (MQ=255)
                                                                                                                                       tAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCa            >  1:4816121/1‑150 (MQ=255)
                                                                                                                                          cgctGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGATTACATTCAAACGGAACGACGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTg         <  2:4654769/147‑1 (MQ=255)
                                                                                                                                           gATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGa        <  1:116343/150‑1 (MQ=255)
                                                                                                                                           gATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTCTCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGa        >  1:2140200/1‑150 (MQ=255)
                                                                                                                                            aTGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGaa       <  2:5137628/150‑1 (MQ=255)
                                                                                                                                            aTGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGaa       <  2:4455625/150‑1 (MQ=255)
                                                                                                                                             tGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGaaa      <  1:1984915/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AGAAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTT  >  CP009273/843151‑843445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGAAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTTTAA  >  CP009273/843151‑843448
                                                                                                                                                    |                                                                                                                                                     
AGAAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCC                                                                                                                                                      >  SRR19973077.365902/1‑150 (MQ=60)
AGAAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATAGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCAC                                                                                                                                                      <  SRR19973077.4287921/150‑1 (MQ=60)
   AATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACA                                                                                                                                                   >  SRR19973077.2173933/1‑150 (MQ=60)
    ATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAG                                                                                                                                                  <  SRR19973077.819075/150‑1 (MQ=60)
      CGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGAT                                                                                                                                                >  SRR19973077.658609/1‑150 (MQ=60)
       GATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATC                                                                                                                                               >  SRR19973077.3637889/1‑150 (MQ=60)
       GATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATC                                                                                                                                               >  SRR19973077.705932/1‑150 (MQ=60)
        ATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCA                                                                                                                                              <  SRR19973077.4889334/150‑1 (MQ=60)
        ATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCA                                                                                                                                              <  SRR19973077.4889805/150‑1 (MQ=60)
          CGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAAC                                                                                                                                            <  SRR19973077.2552052/150‑1 (MQ=60)
            CTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGT                                                                                                                                          >  SRR19973077.4902576/1‑150 (MQ=60)
             TTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTC                                                                                                                                         <  SRR19973077.4308310/150‑1 (MQ=60)
             TTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTC                                                                                                                                         <  SRR19973077.3497519/150‑1 (MQ=60)
             TTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTC                                                                                                                                         <  SRR19973077.2690461/150‑1 (MQ=60)
              TTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCA                                                                                                                                        <  SRR19973077.1448127/150‑1 (MQ=60)
               TTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAA                                                                                                                                       <  SRR19973077.228414/150‑1 (MQ=60)
                 TTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAG                                                                                                                                     >  SRR19973077.1530947/1‑150 (MQ=60)
                 TTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAG                                                                                                                                     >  SRR19973077.4349272/1‑150 (MQ=60)
                 TTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAG                                                                                                                                     >  SRR19973077.4349260/1‑150 (MQ=60)
                     GCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCA                                                                                                                                 <  SRR19973077.3124158/150‑1 (MQ=60)
                      CTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCAC                                                                                                                                >  SRR19973077.853592/1‑150 (MQ=60)
                        CGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACAT                                                                                                                              <  SRR19973077.4569336/150‑1 (MQ=60)
                           CGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATT                                                                                                                           >  SRR19973077.2824360/1‑150 (MQ=60)
                           CGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATT                                                                                                                           <  SRR19973077.450218/150‑1 (MQ=60)
                            GGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTT                                                                                                                          >  SRR19973077.631250/1‑150 (MQ=60)
                              TGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATTATCCCACTGAAATCCATCGGCTTCAGTTAGGAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAAAGTCAAATCCCACAAATTTAT                                                                                                                        >  SRR19973077.893186/1‑150 (MQ=60)
                                ATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCG                                                                                                                      <  SRR19973077.3235225/150‑1 (MQ=60)
                                ATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCG                                                                                                                      <  SRR19973077.3235070/150‑1 (MQ=60)
                                   GTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCC                                                                                                                   <  SRR19973077.4253808/150‑1 (MQ=60)
                                    TAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCT                                                                                                                  <  SRR19973077.1191570/150‑1 (MQ=60)
                                      ATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGT                                                                                                                <  SRR19973077.865849/150‑1 (MQ=60)
                                      ATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGT                                                                                                                <  SRR19973077.2897245/150‑1 (MQ=60)
                                           CGCCAGCGCCAGAACGACGTTTTTGTTTTTCAGTGCCGGAAATATCCCACAGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACAAATATCAAATTCAAATCCCACATATTTAACGCCCacgttaaa                                                                                                           >  SRR19973077.3021946/1‑142 (MQ=60)
                                             CCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATT                                                                                                         <  SRR19973077.3246839/150‑1 (MQ=60)
                                               AGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCA                                                                                                       <  SRR19973077.3897156/150‑1 (MQ=60)
                                                GCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAA                                                                                                      <  SRR19973077.1364781/150‑1 (MQ=60)
                                                GCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAA                                                                                                      <  SRR19973077.2839175/150‑1 (MQ=60)
                                                    CAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGG                                                                                                  >  SRR19973077.1609774/1‑150 (MQ=60)
                                                     AGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGA                                                                                                 >  SRR19973077.5140007/1‑150 (MQ=60)
                                                      GATCGACGTTTTTGGTTTGCAGTGCCGGAATTATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATAGCCCTGTTTAAATTCAAACGGAA                                                                                                >  SRR19973077.3604908/1‑150 (MQ=60)
                                                       ATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAAC                                                                                               <  SRR19973077.1794946/150‑1 (MQ=60)
                                                       ATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAAC                                                                                               <  SRR19973077.4195927/150‑1 (MQ=60)
                                                       ATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAAC                                                                                               <  SRR19973077.4195934/150‑1 (MQ=60)
                                                       ATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACAGAAAACCATCGGCTTCAGTTCCTAATCCAGCGTCAGCACATTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACAAATTTATCGCCCACTTTAAATTCAAACCGAAC                                                                                               >  SRR19973077.3432208/1‑150 (MQ=60)
                                                        TCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACG                                                                                              <  SRR19973077.2313688/150‑1 (MQ=60)
                                                        TCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACG                                                                                              >  SRR19973077.5081467/1‑150 (MQ=60)
                                                        TCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACG                                                                                              <  SRR19973077.2313738/150‑1 (MQ=60)
                                                         CGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGA                                                                                             >  SRR19973077.948224/1‑150 (MQ=60)
                                                         CGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGA                                                                                             >  SRR19973077.950045/1‑150 (MQ=60)
                                                            CGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGG                                                                                          <  SRR19973077.3462125/150‑1 (MQ=60)
                                                             GTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGC                                                                                         >  SRR19973077.26256/1‑150 (MQ=60)
                                                             GTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGC                                                                                         >  SRR19973077.3752604/1‑150 (MQ=60)
                                                                TTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGT                                                                                      <  SRR19973077.4383672/150‑1 (MQ=60)
                                                                  TGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTAT                                                                                    <  SRR19973077.3796782/150‑1 (MQ=60)
                                                                   GGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATC                                                                                   >  SRR19973077.1040349/1‑150 (MQ=60)
                                                                   GGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACG‑AGGCGGTATCC                                                                                  >  SRR19973077.1039981/1‑150 (MQ=60)
                                                                     TTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCG                                                                                 >  SRR19973077.2245458/1‑150 (MQ=60)
                                                                       TGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTC                                                                               <  SRR19973077.1169009/150‑1 (MQ=60)
                                                                        GCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCG                                                                              <  SRR19973077.470514/150‑1 (MQ=60)
                                                                         CAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGACCGAAGGCGGTATCCGTCGC                                                                             >  SRR19973077.4430952/1‑150 (MQ=60)
                                                                          AGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCG                                                                            >  SRR19973077.3896637/1‑150 (MQ=60)
                                                                              CCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAA                                                                        <  SRR19973077.4025983/150‑1 (MQ=60)
                                                                              CCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAA                                                                        <  SRR19973077.4025951/150‑1 (MQ=60)
                                                                               CGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAAC                                                                       >  SRR19973077.2500803/1‑150 (MQ=60)
                                                                                GGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACT                                                                      <  SRR19973077.980854/150‑1 (MQ=60)
                                                                                GGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACT                                                                      <  SRR19973077.980770/150‑1 (MQ=60)
                                                                                 GAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTA                                                                     <  SRR19973077.2154964/150‑1 (MQ=60)
                                                                                      ATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTT                                                                <  SRR19973077.3063632/150‑1 (MQ=60)
                                                                                      ATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTT                                                                <  SRR19973077.2553705/150‑1 (MQ=60)
                                                                                       TCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTT                                                               >  SRR19973077.5114700/1‑150 (MQ=60)
                                                                                         CCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTA                                                             >  SRR19973077.901461/1‑150 (MQ=60)
                                                                                         CCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTA                                                             >  SRR19973077.2321431/1‑150 (MQ=60)
                                                                                           ACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATC                                                           <  SRR19973077.372481/150‑1 (MQ=60)
                                                                                            CTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCC                                                          <  SRR19973077.3221168/150‑1 (MQ=60)
                                                                                            CTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCC                                                          >  SRR19973077.553913/1‑150 (MQ=60)
                                                                                            CTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCC                                                          <  SRR19973077.3220939/150‑1 (MQ=60)
                                                                                            CTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAAAGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATACGTCGCGACAACTAATTTTTTATCC                                                          <  SRR19973077.3140084/150‑1 (MQ=60)
                                                                                             TGATATCCTTCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATTTTTATCGCCCTGTTTAAATTAAAACGGAACGAAGGCGGTTTCCGTCGCGACAACTAATTTTTTATCCG                                                         <  SRR19973077.678004/150‑1 (MQ=60)
                                                                                             TGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCG                                                         >  SRR19973077.4927634/1‑150 (MQ=60)
                                                                                             TGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCG                                                         <  SRR19973077.3335942/150‑1 (MQ=60)
                                                                                             TGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCG                                                         <  SRR19973077.5122061/150‑1 (MQ=60)
                                                                                             TGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCG                                                         >  SRR19973077.1499983/1‑150 (MQ=60)
                                                                                             TGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCG                                                         <  SRR19973077.2113129/150‑1 (MQ=60)
                                                                                               AAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCG                                                       >  SRR19973077.3987456/1‑150 (MQ=60)
                                                                                                 ATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGC                                                     <  SRR19973077.3379413/150‑1 (MQ=60)
                                                                                                 ATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGC                                                     >  SRR19973077.1742677/1‑150 (MQ=60)
                                                                                                 ATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGC                                                     >  SRR19973077.9200/1‑150 (MQ=60)
                                                                                                 ATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGC                                                     >  SRR19973077.8823/1‑150 (MQ=60)
                                                                                                 ATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGC                                                     <  SRR19973077.797102/150‑1 (MQ=60)
                                                                                                  TCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCA                                                    >  SRR19973077.5084771/1‑150 (MQ=60)
                                                                                                  TCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCA                                                    >  SRR19973077.5084769/1‑150 (MQ=60)
                                                                                                   CCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCAT                                                   <  SRR19973077.3241709/150‑1 (MQ=60)
                                                                                                        GGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAA                                              >  SRR19973077.4459439/1‑150 (MQ=60)
                                                                                                        GGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAA                                              >  SRR19973077.1386453/1‑150 (MQ=60)
                                                                                                        GGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAA                                              >  SRR19973077.1386473/1‑150 (MQ=60)
                                                                                                        GGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAA                                              >  SRR19973077.1387188/1‑150 (MQ=60)
                                                                                                         GCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAG                                             <  SRR19973077.3198239/150‑1 (MQ=60)
                                                                                                            TCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAA                                          >  SRR19973077.2793030/1‑150 (MQ=60)
                                                                                                              AGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACC                                        <  SRR19973077.1598622/150‑1 (MQ=60)
                                                                                                                  CGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAA                                    <  SRR19973077.124901/150‑1 (MQ=60)
                                                                                                                   GTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAA                                   >  SRR19973077.4874890/1‑150 (MQ=60)
                                                                                                                   GTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAA                                   <  SRR19973077.4015817/150‑1 (MQ=60)
                                                                                                                    TAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAA                                  >  SRR19973077.4942560/1‑150 (MQ=60)
                                                                                                                    TAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAA                                  >  SRR19973077.1665783/1‑150 (MQ=60)
                                                                                                                     AATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAG                                 >  SRR19973077.4199480/1‑150 (MQ=60)
                                                                                                                       TCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCC                               <  SRR19973077.4932708/150‑1 (MQ=60)
                                                                                                                         CAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTAAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGTCATGAGAAGAAACCGCAAAAGCCAG                             >  SRR19973077.1822856/1‑150 (MQ=60)
                                                                                                                            CTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGT                          <  SRR19973077.2313629/150‑1 (MQ=60)
                                                                                                                              TCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCA                        <  SRR19973077.2475169/150‑1 (MQ=60)
                                                                                                                              TCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCA                        <  SRR19973077.5071125/150‑1 (MQ=60)
                                                                                                                                 GCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTG                     <  SRR19973077.1258412/150‑1 (MQ=60)
                                                                                                                                  CTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGC                    >  SRR19973077.1391461/1‑150 (MQ=60)
                                                                                                                                       TAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCA               >  SRR19973077.4824663/1‑150 (MQ=60)
                                                                                                                                       TAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCA               >  SRR19973077.2274662/1‑150 (MQ=60)
                                                                                                                                         GCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGT             <  SRR19973077.161488/150‑1 (MQ=60)
                                                                                                                                           GATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGA           <  SRR19973077.116575/150‑1 (MQ=60)
                                                                                                                                           GATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTCTCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGA           >  SRR19973077.2143907/1‑150 (MQ=60)
                                                                                                                                             TGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAA         <  SRR19973077.1988383/150‑1 (MQ=60)
                                                                                                                                                CAGCACACAGATCAACGACAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTAATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCACCCAGTGAAACTT      >  SRR19973077.371226/1‑150 (MQ=60)
                                                                                                                                                   CACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTTTA   <  SRR19973077.643670/150‑1 (MQ=60)
                                                                                                                                                    ACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTTTAA  >  SRR19973077.4618599/1‑150 (MQ=60)
                                                                                                                                                    ACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTTTAA  <  SRR19973077.1391124/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
AGAAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTTTAA  >  CP009273/843151‑843448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑