Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 843,299 C→A 57.7% W54C (TGG→TGT)  glnH ← glutamine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273843,2990CA57.7% 45.7 / 243.2 156W54C (TGG→TGT) glnHglutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (26/40);  new base A (36/54);  total (62/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 161 total aligned reads displayed.

AAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTTTA  >  CP009273/843153‑843447
                                                                                                                                                  |                                                                                                                                                    
aaaTCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCacac                                                                                                                                                   <  1:2967450/150‑1 (MQ=255)
aaaTCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCacac                                                                                                                                                   >  1:4756057/1‑150 (MQ=255)
aaaTCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCacac                                                                                                                                                   <  2:4773137/150‑1 (MQ=255)
     gatcgCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTTGTTTGCAGTGCCGGAATGATCCCACAGAAATACATCGGCTTAAGTTCGTAATACACCTTCAGCTCATTAGCGATGGCAGcacacacaaa                                                                                                                                              >  1:3327044/1‑146 (MQ=255)
     gatcgCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTTCAGTGCCGGAATGATCCCACTGAAAACCAAAGGCTTCAGTTCGTAATCCAGCTTCAGCAATTTAGCGATGGCAGCACACAGAta                                                                                                                                              >  1:135374/1‑149 (MQ=255)
      atcgCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCa                                                                                                                                             >  2:2400555/1‑150 (MQ=255)
      atcgCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCa                                                                                                                                             <  2:3952784/150‑1 (MQ=255)
       tcgCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTTGTTTTCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTAAGCTCTTTATCGATGGCAGCACACAGATCaa                                                                                                                                            >  1:656264/1‑150 (MQ=255)
        cgCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAAc                                                                                                                                           <  1:74827/150‑1 (MQ=255)
         gCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACg                                                                                                                                          >  2:3699940/1‑150 (MQ=255)
          cTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGt                                                                                                                                         <  1:1420961/150‑1 (MQ=255)
           ttttttACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGGTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTc                                                                                                                                        >  2:579625/1‑150 (MQ=255)
                tACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATACATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGc                                                                                                                                   >  2:3289104/1‑150 (MQ=255)
                   gCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGcccc                                                                                                                                >  2:748470/1‑149 (MQ=255)
                   gCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCATTAGCGATGGCAGCCCACAGATCAACGTCCAAGCCca                                                                                                                                >  2:697739/1‑150 (MQ=255)
                      cgtcgGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACat                                                                                                                             <  1:4745127/150‑1 (MQ=255)
                      cgtcgGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCGTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACat                                                                                                                             >  2:556955/1‑150 (MQ=255)
                        tcgGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACatat                                                                                                                           <  2:3793375/150‑1 (MQ=255)
                        tcgGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACatat                                                                                                                           <  2:1592283/150‑1 (MQ=255)
                         cgGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATAtt                                                                                                                          <  2:4031215/150‑1 (MQ=255)
                          ggTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATAttt                                                                                                                         >  2:643637/1‑150 (MQ=255)
                          ggTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATAttt                                                                                                                         <  1:2830115/150‑1 (MQ=255)
                            tGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTAt                                                                                                                       <  1:4146981/150‑1 (MQ=255)
                              aTGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCg                                                                                                                     <  1:1488609/150‑1 (MQ=255)
                              aTGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATGTATCg                                                                                                                     <  2:830670/150‑1 (MQ=255)
                              aTGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTATTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATATATCg                                                                                                                     <  2:1780795/150‑1 (MQ=255)
                                ggTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACAGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGGCAAAGCCCACATATTTATCGcc                                                                                                                   >  1:2495730/1‑150 (MQ=255)
                                  tAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCt                                                                                                                 <  1:3229963/150‑1 (MQ=255)
                                   aaTGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTg                                                                                                                <  1:2167253/150‑1 (MQ=255)
                                   aaTGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTg                                                                                                                <  1:2157464/150‑1 (MQ=255)
                                            cagcgccagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAACTCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTc                                                                                                       <  2:2634990/150‑1 (MQ=255)
                                               cgccagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCaaa                                                                                                    <  2:2168088/150‑1 (MQ=255)
                                               cgccagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCaaa                                                                                                    <  2:2168985/150‑1 (MQ=255)
                                               cgccagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCaaa                                                                                                    >  2:2820527/1‑150 (MQ=255)
                                                 ccagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACg                                                                                                  <  1:1414596/150‑1 (MQ=255)
                                                      tCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACg                                                                                             <  1:965068/150‑1 (MQ=255)
                                                       cGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGa                                                                                            >  1:4313076/1‑150 (MQ=255)
                                                         aCGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAg                                                                                          <  1:2085218/150‑1 (MQ=255)
                                                          cGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAgg                                                                                         >  1:4080800/1‑150 (MQ=255)
                                                           gTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGc                                                                                        >  2:665849/1‑150 (MQ=255)
                                                           gTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGc                                                                                        >  2:665934/1‑150 (MQ=255)
                                                             ttttGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCgg                                                                                      <  1:1284992/150‑1 (MQ=255)
                                                             ttttGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCgg                                                                                      >  1:1576746/1‑150 (MQ=255)
                                                               ttGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTa                                                                                    <  1:2678681/150‑1 (MQ=255)
                                                               ttGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTa                                                                                    <  1:2678378/150‑1 (MQ=255)
                                                                  gTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATcc                                                                                 <  2:4766810/150‑1 (MQ=255)
                                                                  gTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATcc                                                                                 <  2:1926246/150‑1 (MQ=255)
                                                                  gTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATcc                                                                                 <  2:413926/150‑1 (MQ=255)
                                                                         gTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGa                                                                          >  2:4359859/1‑150 (MQ=255)
                                                                          tGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGAc                                                                         <  2:3046932/150‑1 (MQ=255)
                                                                          tGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGAc                                                                         <  1:4418810/150‑1 (MQ=255)
                                                                            ccGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACaa                                                                       >  1:1760059/1‑150 (MQ=255)
                                                                             cGGAATGATCCAACTGAAATCCATCGGCTACAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACaaa                                                                      >  1:1274846/1‑149 (MQ=255)
                                                                              ggAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACt                                                                     >  1:4163232/1‑150 (MQ=255)
                                                                                aaTGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTaa                                                                   <  1:4423193/150‑1 (MQ=255)
                                                                                 aTGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAt                                                                  <  1:1753136/150‑1 (MQ=255)
                                                                                 aTGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAt                                                                  <  1:4205449/150‑1 (MQ=255)
                                                                                 aTGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAt                                                                  <  1:4205489/150‑1 (MQ=255)
                                                                                  tGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAtt                                                                 <  2:1769688/150‑1 (MQ=255)
                                                                                  tGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAtt                                                                 <  1:4066816/150‑1 (MQ=255)
                                                                                   gATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAttt                                                                >  1:2699369/1‑150 (MQ=255)
                                                                                    aTCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAtttt                                                               <  1:246467/150‑1 (MQ=255)
                                                                                       ccACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTa                                                            <  2:3263722/150‑1 (MQ=255)
                                                                                        cACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTAt                                                           >  2:407270/1‑150 (MQ=255)
                                                                                               aTCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGc                                                    >  1:1434229/1‑150 (MQ=255)
                                                                                                tCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCa                                                   >  1:4671480/1‑150 (MQ=255)
                                                                                                 ccATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCAt                                                  <  2:2336562/150‑1 (MQ=255)
                                                                                                 ccATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCAt                                                  >  1:2051879/1‑150 (MQ=255)
                                                                                                  cATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATg                                                 <  1:1397235/150‑1 (MQ=255)
                                                                                                    tCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGAGTACTAATTTTTTATCCGCGGCATgag                                               >  1:4530855/1‑150 (MQ=255)
                                                                                                      ggCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACACCTAATTTTTTATCCGCGGCATGagaa                                             >  2:2377597/1‑150 (MQ=255)
                                                                                                         ttCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAgaagaa                                          >  1:1224296/1‑150 (MQ=255)
                                                                                                         ttCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAgaagaa                                          <  2:3833151/150‑1 (MQ=255)
                                                                                                         ttCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAgaagaa                                          >  2:3217734/1‑150 (MQ=255)
                                                                                                         ttCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAgaagaa                                          >  2:3572193/1‑150 (MQ=255)
                                                                                                           cAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAAc                                        >  2:2835922/1‑150 (MQ=255)
                                                                                                           cAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAAc                                        >  2:2836287/1‑150 (MQ=255)
                                                                                                            aGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAAcc                                       <  1:643637/150‑1 (MQ=255)
                                                                                                              ttCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGc                                     >  1:2646705/1‑150 (MQ=255)
                                                                                                                cGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCaa                                   >  1:788471/1‑150 (MQ=255)
                                                                                                                cGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCaa                                   >  1:788424/1‑150 (MQ=255)
                                                                                                                  tAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCaaaa                                 <  2:3432486/150‑1 (MQ=255)
                                                                                                                   aaTCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAg                                >  1:2436712/1‑150 (MQ=255)
                                                                                                                      ccAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCa                             <  1:2059318/150‑1 (MQ=255)
                                                                                                                      ccAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCa                             <  1:2295510/150‑1 (MQ=255)
                                                                                                                      ccAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCa                             <  1:2295436/150‑1 (MQ=255)
                                                                                                                       cAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAg                            <  2:2395044/150‑1 (MQ=255)
                                                                                                                            tCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCa                       <  1:2645557/150‑1 (MQ=255)
                                                                                                                             cAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAg                      >  2:3080596/1‑150 (MQ=255)
                                                                                                                               gCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTg                    <  2:4002972/150‑1 (MQ=255)
                                                                                                                                 tctTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCa                  <  1:879126/150‑1 (MQ=255)
                                                                                                                                 tctTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCa                  <  2:4393293/150‑1 (MQ=255)
                                                                                                                                 tctTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCa                  <  2:4393913/150‑1 (MQ=255)
                                                                                                                                    tttgCGATGGCAGCACACAGTTCAACGTCAAAGCCCACATATTTATGGCCCTGTTTTATTTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTACTTTTTTAGCCTCGGCATGAGACGAAACCCCAAAAGCCAGGGTCAGTGCAGcc               <  2:1445590/147‑1 (MQ=255)
                                                                                                                                     tAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGACGAAACCGCAAAAGCCAGGGTCAGTGCAGCCa              <  1:368567/150‑1 (MQ=255)
                                                                                                                                          aTGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGaa         <  1:2400555/150‑1 (MQ=255)
                                                                                                                                           tGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGaaa        >  1:4810118/1‑150 (MQ=255)
                                                                                                                                            ggCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAAc       <  2:744701/150‑1 (MQ=255)
                                                                                                                                            ggCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAAc       <  1:407270/150‑1 (MQ=255)
                                                                                                                                                 cccACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTTTa  >  2:1690774/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTTTA  >  CP009273/843153‑843447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTT  >  CP009273/843153‑843444
                                                                                                                                                  |                                                                                                                                                 
AAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACAC                                                                                                                                                >  SRR19973081.4784816/1‑150 (MQ=60)
AAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACAC                                                                                                                                                <  SRR19973081.2985734/150‑1 (MQ=60)
AAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACAGAAATCCAACGGCTTCAGTTCGTAATCCAGCTTAAGCACTTTAGCGCTGGCAGCCCAC                                                                                                                                                >  SRR19973081.1419499/1‑150 (MQ=60)
  ATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTTCAGTGCCGGAATGATACCACAGAAATACATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAG                                                                                                                                              >  SRR19973081.4207731/1‑150 (MQ=60)
     GATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTTCAGTGCCGGAATGATCCCACTGAAAACCAAAGGCTTCAGTTCGTAATCCAGCTTCAGCAATTTAGCGATGGCAGCACACAGATA                                                                                                                                           >  SRR19973081.136659/1‑150 (MQ=60)
       TCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTTGTTTTCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTAAGCTCTTTATCGATGGCAGCACACAGATCAA                                                                                                                                         >  SRR19973081.660764/1‑150 (MQ=60)
        CGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAAC                                                                                                                                        <  SRR19973081.75667/150‑1 (MQ=60)
          CTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGT                                                                                                                                      <  SRR19973081.1430551/150‑1 (MQ=60)
          CTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGT                                                                                                                                      <  SRR19973081.1430477/150‑1 (MQ=60)
             TTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAA                                                                                                                                   <  SRR19973081.4535333/150‑1 (MQ=60)
                      CGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACAT                                                                                                                          <  SRR19973081.2357145/150‑1 (MQ=60)
                      CGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACAT                                                                                                                          <  SRR19973081.4773828/150‑1 (MQ=60)
                        TCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATAT                                                                                                                        <  SRR19973081.249771/150‑1 (MQ=60)
                          GGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTT                                                                                                                      <  SRR19973081.2847268/150‑1 (MQ=60)
                          GGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTT                                                                                                                      <  SRR19973081.2016963/150‑1 (MQ=60)
                            TGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTAT                                                                                                                    <  SRR19973081.4172332/150‑1 (MQ=60)
                            TGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTAT                                                                                                                    <  SRR19973081.4172306/150‑1 (MQ=60)
                              ATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCG                                                                                                                  <  SRR19973081.1498458/150‑1 (MQ=60)
                              ATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCG                                                                                                                  <  SRR19973081.2659494/150‑1 (MQ=60)
                                GGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACAGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGGCAAAGCCCACATATTTATCGCC                                                                                                                >  SRR19973081.2511279/1‑150 (MQ=60)
                                  TAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCT                                                                                                              <  SRR19973081.3249437/150‑1 (MQ=60)
                                   AATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTG                                                                                                             <  SRR19973081.2171205/150‑1 (MQ=60)
                                   AATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTG                                                                                                             <  SRR19973081.2181046/150‑1 (MQ=60)
                                       CCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTA                                                                                                         <  SRR19973081.156526/150‑1 (MQ=60)
                                            CAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTC                                                                                                    <  SRR19973081.765332/150‑1 (MQ=60)
                                                 CCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACG                                                                                               <  SRR19973081.1424074/150‑1 (MQ=60)
                                                   AGATCGACGTTTTTTGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTAAGCTCTTTAGAGATGGCAGCACACAGATCAACGTCAAAGCCCACAAATTTATAGCCCAGTTTAAATTCAAACGGA                                                                                             >  SRR19973081.2710107/1‑150 (MQ=60)
                                                      TCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACG                                                                                          <  SRR19973081.971634/150‑1 (MQ=60)
                                                       CGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTATCGATGGCAGCACACAGATCAACGTCAAATCCCACAAATTTATCGCCCTGTTTAAAATAAAACGGAACGA                                                                                         >  SRR19973081.4339238/1‑150 (MQ=60)
                                                       CGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGA                                                                                         >  SRR19973081.4339311/1‑150 (MQ=60)
                                                         ACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAG                                                                                       <  SRR19973081.2098469/150‑1 (MQ=60)
                                                          CGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGG                                                                                      >  SRR19973081.4105547/1‑150 (MQ=60)
                                                          CGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGG                                                                                      >  SRR19973081.4105543/1‑150 (MQ=60)
                                                          CGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGG                                                                                      <  SRR19973081.4437820/150‑1 (MQ=60)
                                                             TTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGG                                                                                   >  SRR19973081.4700663/1‑150 (MQ=60)
                                                             TTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGG                                                                                   <  SRR19973081.1293521/150‑1 (MQ=60)
                                                             TTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGG                                                                                   >  SRR19973081.1586921/1‑150 (MQ=60)
                                                               TTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTA                                                                                 <  SRR19973081.2694998/150‑1 (MQ=60)
                                                               TTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTA                                                                                 <  SRR19973081.2695301/150‑1 (MQ=60)
                                                                          TGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGAC                                                                      <  SRR19973081.4445407/150‑1 (MQ=60)
                                                                            CCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAA                                                                    >  SRR19973081.1771582/1‑150 (MQ=60)
                                                                             CGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAAC                                                                   >  SRR19973081.1285780/1‑150 (MQ=60)
                                                                             CGGAATGATCCAACTGAAATCCATCGGCTACAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAAA                                                                   >  SRR19973081.1283294/1‑150 (MQ=60)
                                                                              GGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACT                                                                  >  SRR19973081.4188680/1‑150 (MQ=60)
                                                                              GGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACT                                                                  >  SRR19973081.4782852/1‑150 (MQ=60)
                                                                                AATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAA                                                                <  SRR19973081.4449813/150‑1 (MQ=60)
                                                                                 ATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAT                                                               <  SRR19973081.4231187/150‑1 (MQ=60)
                                                                                 ATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAT                                                               <  SRR19973081.4231147/150‑1 (MQ=60)
                                                                                 ATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAT                                                               <  SRR19973081.1764625/150‑1 (MQ=60)
                                                                                 ATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAT                                                               <  SRR19973081.2416554/150‑1 (MQ=60)
                                                                                  TGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATT                                                              <  SRR19973081.4091418/150‑1 (MQ=60)
                                                                                   GATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTT                                                             >  SRR19973081.2716079/1‑150 (MQ=60)
                                                                                    ATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTT                                                            <  SRR19973081.3993271/150‑1 (MQ=60)
                                                                                    ATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTT                                                            <  SRR19973081.248487/150‑1 (MQ=60)
                                                                                               ATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGC                                                 >  SRR19973081.1443812/1‑150 (MQ=60)
                                                                                                TCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCA                                                >  SRR19973081.4699831/1‑150 (MQ=60)
                                                                                                TCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCA                                                >  SRR19973081.697899/1‑150 (MQ=60)
                                                                                                 CCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCAT                                               >  SRR19973081.2064814/1‑150 (MQ=60)
                                                                                                  CATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATG                                              <  SRR19973081.1406602/150‑1 (MQ=60)
                                                                                                   ATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGA                                             >  SRR19973081.1653777/1‑150 (MQ=60)
                                                                                                    TCGGCTTCAGTTCGTACTCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAG                                            <  SRR19973081.583731/150‑1 (MQ=60)
                                                                                                    TCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGAGTACTAATTTTTTATCCGCGGCATGAG                                            >  SRR19973081.4558294/1‑150 (MQ=60)
                                                                                                         TTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAA                                       >  SRR19973081.1232290/1‑150 (MQ=60)
                                                                                                         TTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAA                                       <  SRR19973081.2118800/150‑1 (MQ=60)
                                                                                                            AGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACC                                    <  SRR19973081.648069/150‑1 (MQ=60)
                                                                                                              TTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGC                                  >  SRR19973081.2663173/1‑150 (MQ=60)
                                                                                                                CGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAA                                >  SRR19973081.793472/1‑150 (MQ=60)
                                                                                                                CGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAA                                >  SRR19973081.793424/1‑150 (MQ=60)
                                                                                                                   AATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAG                             >  SRR19973081.2451699/1‑150 (MQ=60)
                                                                                                                      CCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCA                          <  SRR19973081.2309858/150‑1 (MQ=60)
                                                                                                                      CCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCA                          <  SRR19973081.2072318/150‑1 (MQ=60)
                                                                                                                      CCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCA                          <  SRR19973081.2309933/150‑1 (MQ=60)
                                                                                                                      CCAGCTTCAGCTATTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCA                          <  SRR19973081.2072775/150‑1 (MQ=60)
                                                                                                                            TCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCA                    <  SRR19973081.2662023/150‑1 (MQ=60)
                                                                                                                            TCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCA                    <  SRR19973081.2663782/150‑1 (MQ=60)
                                                                                                                             CAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAG                   <  SRR19973081.3550231/150‑1 (MQ=60)
                                                                                                                                 TCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCA               <  SRR19973081.884898/150‑1 (MQ=60)
                                                                                                                                 TCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCA               <  SRR19973081.884960/150‑1 (MQ=60)
                                                                                                                                 TCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCA               >  SRR19973081.2288710/1‑150 (MQ=60)
                                                                                                                                  CTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAG              >  SRR19973081.1396688/1‑150 (MQ=60)
                                                                                                                                    TTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCC            >  SRR19973081.3058982/1‑150 (MQ=60)
                                                                                                                                     TAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGACGAAACCGCAAAAGCCAGGGTCAGTGCAGCCA           <  SRR19973081.371039/150‑1 (MQ=60)
                                                                                                                                          ATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAA      <  SRR19973081.2415339/150‑1 (MQ=60)
                                                                                                                                           TGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAA     >  SRR19973081.4839082/1‑150 (MQ=60)
                                                                                                                                            GGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAAC    <  SRR19973081.409856/150‑1 (MQ=60)
                                                                                                                                              CAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTAATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTT  <  SRR19973081.2837656/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                 
AAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTT  >  CP009273/843153‑843444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑