Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 843,299 C→A 90.7% W54C (TGG→TGT)  glnH ← glutamine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273843,2990CA90.7% 597.0 / 49.1 201W54C (TGG→TGT) glnHglutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (8/11);  new base A (69/113);  total (77/124)
Fisher's exact test for biased strand distribution p-value = 8.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 212 total aligned reads displayed.

GAAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTTT  >  CP009273/843152‑843446
                                                                                                                                                   |                                                                                                                                                   
gaAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATTATCCCACAGTAATCCATCGGCTTCAGTTCGTAATCCAGCTTAAGCTCTTTAGCGATGGCAGcaca                                                                                                                                                   >  1:772201/1‑150 (MQ=255)
gaAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGcaca                                                                                                                                                   >  2:3733869/1‑150 (MQ=255)
gaAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCAACCGCTTCAGTTAGTAATCCAGCTTCAGCTCGTTATCGATGGCAGcaca                                                                                                                                                   >  1:2208154/1‑150 (MQ=255)
 aaaTCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCCGCTTCAGCTCTTTAGCGATGGCAGCacac                                                                                                                                                  >  2:1075772/1‑150 (MQ=255)
    tcgatcgCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATACCACTGAAATCCATCGGCTTAATTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGa                                                                                                                                               >  1:582382/1‑150 (MQ=255)
      gatcgCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATc                                                                                                                                             >  2:2230160/1‑150 (MQ=255)
       atcgCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTTGTTTTCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCa                                                                                                                                            >  2:1809036/1‑150 (MQ=255)
       atcgCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCa                                                                                                                                            <  1:2380486/150‑1 (MQ=255)
        tcgCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTTCAGTTCCGGAATGATCCCACTGAAATCCAACGGCTTCAGTTAGTAAAACAGCTTAATCTCTTTAGCGATGGCAGCACACAGATCaa                                                                                                                                           >  1:1488693/1‑150 (MQ=255)
        tcgCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCaa                                                                                                                                           >  2:4181880/1‑150 (MQ=255)
        tcgCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATACCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCaa                                                                                                                                           >  1:3082295/1‑150 (MQ=255)
           cTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGt                                                                                                                                        <  2:401789/150‑1 (MQ=255)
              ttttACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCaa                                                                                                                                     <  1:3608190/150‑1 (MQ=255)
                  aCGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGcc                                                                                                                                 <  2:3909321/150‑1 (MQ=255)
                  aCGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGcc                                                                                                                                 <  2:3122408/150‑1 (MQ=255)
                   cGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGccc                                                                                                                                <  1:2685449/150‑1 (MQ=255)
                   cGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGccc                                                                                                                                >  2:1864607/1‑150 (MQ=255)
                    gCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCca                                                                                                                               <  2:639483/150‑1 (MQ=255)
                     cTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCcac                                                                                                                              <  1:491141/150‑1 (MQ=255)
                        gtcgGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACata                                                                                                                           >  2:2681032/1‑150 (MQ=255)
                          cgGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATAtt                                                                                                                         <  1:3021536/150‑1 (MQ=255)
                           ggTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATAttt                                                                                                                        <  2:3484858/150‑1 (MQ=255)
                           ggTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATAttt                                                                                                                        >  2:2949832/1‑150 (MQ=255)
                           ggTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATAttt                                                                                                                        <  2:1806669/150‑1 (MQ=255)
                              gATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATc                                                                                                                     <  2:3300854/150‑1 (MQ=255)
                              gATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCAACGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATc                                                                                                                     >  1:181970/1‑150 (MQ=255)
                                tGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGc                                                                                                                   <  2:3911964/150‑1 (MQ=255)
                                   tAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCt                                                                                                                <  1:994857/150‑1 (MQ=255)
                                   tAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCt                                                                                                                <  1:3189156/150‑1 (MQ=255)
                                    aaTGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTg                                                                                                               >  2:3007567/1‑150 (MQ=255)
                                     aTGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGt                                                                                                              <  2:3128341/150‑1 (MQ=255)
                                      tGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGtt                                                                                                             <  1:498539/150‑1 (MQ=255)
                                       gCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGttt                                                                                                            <  1:3817942/150‑1 (MQ=255)
                                       gCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGttt                                                                                                            <  1:2948900/150‑1 (MQ=255)
                                        cccGCCAGCGCCAGATCGACGTTTTTGGTTTTCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTa                                                                                                           <  1:2709360/150‑1 (MQ=255)
                                        cccGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTa                                                                                                           <  1:1291788/150‑1 (MQ=255)
                                         ccGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTaa                                                                                                          <  1:3579426/150‑1 (MQ=255)
                                           gccagcgccagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAAt                                                                                                        <  1:3050812/150‑1 (MQ=255)
                                              agcgccagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCa                                                                                                     <  2:2088488/150‑1 (MQ=255)
                                              agcgccagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCa                                                                                                     <  2:4274805/150‑1 (MQ=255)
                                                  ccagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACg                                                                                                 <  1:721708/150‑1 (MQ=255)
                                                   cagATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACgg                                                                                                >  2:2782664/1‑150 (MQ=255)
                                                    agATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGa                                                                                               <  2:4278525/150‑1 (MQ=255)
                                                        cGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGa                                                                                           <  1:2879499/150‑1 (MQ=255)
                                                         gACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAATCGGAACGaa                                                                                          >  2:2309211/1‑150 (MQ=255)
                                                         gACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGaa                                                                                          >  1:4244633/1‑150 (MQ=255)
                                                            gTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGc                                                                                       >  2:2403476/1‑150 (MQ=255)
                                                              ttttGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCgg                                                                                     >  1:947910/1‑150 (MQ=255)
                                                               tttGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCTGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGt                                                                                    <  1:2549774/150‑1 (MQ=255)
                                                                 tGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTAt                                                                                  >  1:3622415/1‑150 (MQ=255)
                                                                 tGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTAt                                                                                  <  2:2952593/150‑1 (MQ=255)
                                                                  ggTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATc                                                                                 <  1:1536937/150‑1 (MQ=255)
                                                                  ggTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATc                                                                                 >  1:1225116/1‑150 (MQ=255)
                                                                  ggTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATc                                                                                 >  1:3416715/1‑150 (MQ=255)
                                                                     ttGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGt                                                                              <  1:1009448/150‑1 (MQ=255)
                                                                      tGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTc                                                                             <  2:712727/150‑1 (MQ=255)
                                                                      tGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTc                                                                             <  2:1272622/150‑1 (MQ=255)
                                                                       gCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGAGGGCAGCACACAGATCAACGTCAAAGCCCACATATTTAGCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTcg                                                                            <  1:3279313/150‑1 (MQ=255)
                                                                         aGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTcgcg                                                                          >  1:1899220/1‑150 (MQ=255)
                                                                         aGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTcgcg                                                                          <  2:1060011/150‑1 (MQ=255)
                                                                         aGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTcgcg                                                                          >  2:3833232/1‑150 (MQ=255)
                                                                               ggAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACt                                                                    >  1:1712650/1‑150 (MQ=255)
                                                                               ggAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACt                                                                    >  1:2236182/1‑150 (MQ=255)
                                                                                 aaTGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTaa                                                                  >  1:2581886/1‑150 (MQ=255)
                                                                                    gATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAttt                                                               >  1:3867602/1‑150 (MQ=255)
                                                                                    gATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAAttt                                                               <  1:1787942/150‑1 (MQ=255)
                                                                                       cccACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACGAAtttttt                                                            <  2:1693605/150‑1 (MQ=255)
                                                                                          aCTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATc                                                         <  1:3856027/150‑1 (MQ=255)
                                                                                             gAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCcgc                                                      >  1:2133185/1‑150 (MQ=255)
                                                                                              aaaTCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCcgcg                                                     <  2:1456278/150‑1 (MQ=255)
                                                                                               aaTCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCgg                                                    <  2:2822969/150‑1 (MQ=255)
                                                                                                  ccATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCAt                                                 >  2:3812544/1‑150 (MQ=255)
                                                                                                    aTCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATga                                               >  1:2657294/1‑150 (MQ=255)
                                                                                                    aTCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATga                                               >  1:127209/1‑150 (MQ=255)
                                                                                                    aTCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATAAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATga                                               <  1:1075428/150‑1 (MQ=255)
                                                                                                       ggCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGagaa                                            <  1:4173309/150‑1 (MQ=255)
                                                                                                         cTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCATCGCGACAACTAATTTTTTATCCGCGGCATGagaaga                                          <  2:1788428/150‑1 (MQ=255)
                                                                                                            cAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAAc                                       <  2:1295496/150‑1 (MQ=255)
                                                                                                              gTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCg                                     >  2:557153/1‑150 (MQ=255)
                                                                                                                    aaTCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAg                               <  1:2523598/150‑1 (MQ=255)
                                                                                                                      tCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGcc                             <  2:562745/150‑1 (MQ=255)
                                                                                                                      tCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGcc                             >  2:1541678/1‑150 (MQ=255)
                                                                                                                        cAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACACCTAATTTTTTATCCGCGGCATGAGACGAAACCGCAAAAGCCAg                           <  1:1990518/150‑1 (MQ=255)
                                                                                                                         aGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAgg                          >  2:3352334/1‑150 (MQ=255)
                                                                                                                         aGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAgg                          >  2:594884/1‑150 (MQ=255)
                                                                                                                           cTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGt                        <  1:4106164/150‑1 (MQ=255)
                                                                                                                           cTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGt                        >  1:535490/1‑150 (MQ=255)
                                                                                                                             tCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCa                      >  1:1606973/1‑150 (MQ=255)
                                                                                                                              cAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAg                     <  1:380564/150‑1 (MQ=255)
                                                                                                                               aGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGt                    <  1:1373826/150‑1 (MQ=255)
                                                                                                                               aGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGt                    <  2:2450502/150‑1 (MQ=255)
                                                                                                                               aGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGt                    <  1:4266087/150‑1 (MQ=255)
                                                                                                                                 ctctTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGc                  <  2:3698339/150‑1 (MQ=255)
                                                                                                                                  tctTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTTTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCa                 <  1:69748/150‑1 (MQ=255)
                                                                                                                                    tttAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGc               >  1:4203670/1‑150 (MQ=255)
                                                                                                                                         cGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTg          <  2:1408109/150‑1 (MQ=255)
                                                                                                                                          gATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGa         >  1:3717741/1‑150 (MQ=255)
                                                                                                                                           aTGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAACCCGCAAAAGCCAGGGTCAGTGCAGCCAGTGaa        <  2:253303/150‑1 (MQ=255)
                                                                                                                                               cAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACtt    >  1:2798948/1‑150 (MQ=255)
                                                                                                                                               cAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACtt    >  1:1076855/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GAAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTTT  >  CP009273/843152‑843446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTTTA  >  CP009273/843152‑843447
                                                                                                                                                   |                                                                                                                                                    
GAAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATTATCCCACAGTAATCCATCGGCTTCAGTTCGTAATCCAGCTTAAGCTCTTTAGCGATGGCAGCACA                                                                                                                                                    >  SRR19973079.776512/1‑150 (MQ=60)
GAAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATACAGCTTCAGCTCTTTATCGATGGCAGCACA                                                                                                                                                    >  SRR19973079.2219125/1‑150 (MQ=60)
GAAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCAACCGCTTCAGTTAGTAATCCAGCTTCAGCTCGTTATCGATGGCAGCACA                                                                                                                                                    >  SRR19973079.2219547/1‑150 (MQ=60)
 AAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACAC                                                                                                                                                   <  SRR19973079.3500385/150‑1 (MQ=60)
 AAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACAC                                                                                                                                                   <  SRR19973079.1975417/150‑1 (MQ=60)
    TCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATACCACTGAAATCCATCGGCTTAATTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGA                                                                                                                                                >  SRR19973079.585807/1‑150 (MQ=60)
       ATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCA                                                                                                                                             <  SRR19973079.2392805/150‑1 (MQ=60)
        TCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTTCAGTTCCGGAATGATCCCACTGAAATCCAACGGCTTCAGTTAGTAAAACAGCTTAATCTCTTTAGCGATGGCAGCACACAGATCAA                                                                                                                                            >  SRR19973079.1496866/1‑150 (MQ=60)
        TCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATACCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAA                                                                                                                                            >  SRR19973079.3098236/1‑150 (MQ=60)
         CGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAAC                                                                                                                                           <  SRR19973079.3215038/150‑1 (MQ=60)
          GCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACG                                                                                                                                          <  SRR19973079.2094133/150‑1 (MQ=60)
              TTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAA                                                                                                                                      <  SRR19973079.3626488/150‑1 (MQ=60)
                   CGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCC                                                                                                                                 <  SRR19973079.2699358/150‑1 (MQ=60)
                     CTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCAC                                                                                                                               >  SRR19973079.254948/1‑150 (MQ=60)
                     CTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCAC                                                                                                                               <  SRR19973079.494606/150‑1 (MQ=60)
                     CTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCAC                                                                                                                               <  SRR19973079.494124/150‑1 (MQ=60)
                      TCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACA                                                                                                                              <  SRR19973079.1144060/150‑1 (MQ=60)
                      TCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACA                                                                                                                              <  SRR19973079.3062424/150‑1 (MQ=60)
                      TCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACA                                                                                                                              <  SRR19973079.739462/150‑1 (MQ=60)
                      TCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACA                                                                                                                              <  SRR19973079.4279695/150‑1 (MQ=60)
                         TCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATAT                                                                                                                           <  SRR19973079.4211777/150‑1 (MQ=60)
                          CGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATT                                                                                                                          <  SRR19973079.3037156/150‑1 (MQ=60)
                          CGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATT                                                                                                                          <  SRR19973079.1690741/150‑1 (MQ=60)
                           GGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTT                                                                                                                         >  SRR19973079.1551215/1‑150 (MQ=60)
                              GATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATC                                                                                                                      <  SRR19973079.4335455/150‑1 (MQ=60)
                              GATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCAACGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATC                                                                                                                      >  SRR19973079.183315/1‑150 (MQ=60)
                                 GGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCC                                                                                                                   <  SRR19973079.3181818/150‑1 (MQ=60)
                                  GTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCC                                                                                                                  <  SRR19973079.3355041/150‑1 (MQ=60)
                                   TAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCT                                                                                                                 <  SRR19973079.1000483/150‑1 (MQ=60)
                                   TAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCT                                                                                                                 <  SRR19973079.3205447/150‑1 (MQ=60)
                                     ATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGT                                                                                                               >  SRR19973079.139718/1‑150 (MQ=60)
                                      TGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTT                                                                                                              >  SRR19973079.2810718/1‑150 (MQ=60)
                                      TGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTT                                                                                                              <  SRR19973079.501573/150‑1 (MQ=60)
                                       GCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTT                                                                                                             <  SRR19973079.3837672/150‑1 (MQ=60)
                                       GCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTT                                                                                                             <  SRR19973079.3837478/150‑1 (MQ=60)
                                       GCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTT                                                                                                             <  SRR19973079.2964024/150‑1 (MQ=60)
                                       GCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTT                                                                                                             <  SRR19973079.1613537/150‑1 (MQ=60)
                                        CCCGCCAGCGCCAGATCGACGTTTTTGGTTTTCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTA                                                                                                            <  SRR19973079.2723374/150‑1 (MQ=60)
                                        CCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTA                                                                                                            <  SRR19973079.1299133/150‑1 (MQ=60)
                                        CCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTA                                                                                                            <  SRR19973079.641708/150‑1 (MQ=60)
                                         CCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAA                                                                                                           <  SRR19973079.3597660/150‑1 (MQ=60)
                                         CCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAA                                                                                                           <  SRR19973079.3597666/150‑1 (MQ=60)
                                           GCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCTTCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAAT                                                                                                         <  SRR19973079.2696332/150‑1 (MQ=60)
                                           GCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAAT                                                                                                         <  SRR19973079.3066611/150‑1 (MQ=60)
                                            CCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATT                                                                                                        >  SRR19973079.3830380/1‑150 (MQ=60)
                                                  CCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACG                                                                                                  <  SRR19973079.725553/150‑1 (MQ=60)
                                                    AGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGA                                                                                                <  SRR19973079.2635619/150‑1 (MQ=60)
                                                     GATCGACGTTTTTGGTTTTCAGTGCCGGAAAGATCCCACAGAAATCCAAAGGCTTAAGTTAGTAAACCACCATCATCTATTTATCGATGGCAGCACACAGATCAACGTCAAAGCCCACAAATTTATAGACCTGTTTAAATTaaaaacgaa                                                                                               >  SRR19973079.3437733/1‑141 (MQ=49)
                                                        CGACGTTTTTTGTTTGCAGTGCCGGAATGATCCCACAGAAATCCATCGGCTTCAGTTAGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATAAACGTCAAAGCCCACAAATTTATAGCCCAGTTTAAATTAAAACGGAACGA                                                                                            >  SRR19973079.1524988/1‑150 (MQ=60)
                                                        CGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGA                                                                                            <  SRR19973079.3793390/150‑1 (MQ=60)
                                                        CGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGA                                                                                            <  SRR19973079.2894071/150‑1 (MQ=60)
                                                         GACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAA                                                                                           >  SRR19973079.4266115/1‑150 (MQ=60)
                                                         GACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAA                                                                                           >  SRR19973079.4264628/1‑150 (MQ=60)
                                                          ACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAG                                                                                          >  SRR19973079.106370/1‑150 (MQ=60)
                                                           CGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGG                                                                                         >  SRR19973079.1285352/1‑150 (MQ=60)
                                                            GTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGC                                                                                        <  SRR19973079.2400787/150‑1 (MQ=60)
                                                              TTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGG                                                                                      >  SRR19973079.953342/1‑150 (MQ=60)
                                                               TTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCTGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGT                                                                                     <  SRR19973079.2562675/150‑1 (MQ=60)
                                                                 TGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTAT                                                                                   >  SRR19973079.3640742/1‑150 (MQ=60)
                                                                 TGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTAT                                                                                   >  SRR19973079.560559/1‑150 (MQ=60)
                                                                  GGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATC                                                                                  >  SRR19973079.3434358/1‑150 (MQ=60)
                                                                  GGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATC                                                                                  >  SRR19973079.1232153/1‑150 (MQ=60)
                                                                  GGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATC                                                                                  <  SRR19973079.1545420/150‑1 (MQ=60)
                                                                   GTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCC                                                                                 <  SRR19973079.893381/150‑1 (MQ=60)
                                                                   GGTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCC                                                                                 >  SRR19973079.1276821/1‑150 (MQ=60)
                                                                     TTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGT                                                                               <  SRR19973079.1015129/150‑1 (MQ=60)
                                                                      TGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTC                                                                              <  SRR19973079.4128015/150‑1 (MQ=60)
                                                                      TGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTC                                                                              <  SRR19973079.1816606/150‑1 (MQ=60)
                                                                       GCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGAGGGCAGCACACAGATCAACGTCAAAGCCCACATATTTAGCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCG                                                                             <  SRR19973079.3295934/150‑1 (MQ=60)
                                                                         AGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCG                                                                           >  SRR19973079.1909330/1‑150 (MQ=60)
                                                                               GGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACT                                                                     >  SRR19973079.2247755/1‑150 (MQ=60)
                                                                               GGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACT                                                                     >  SRR19973079.1721797/1‑150 (MQ=60)
                                                                               GGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACT                                                                     >  SRR19973079.3670370/1‑150 (MQ=60)
                                                                               GGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACT                                                                     >  SRR19973079.2245901/1‑150 (MQ=60)
                                                                               GGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACT                                                                     >  SRR19973079.2245890/1‑150 (MQ=60)
                                                                                 AATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAA                                                                   >  SRR19973079.271548/1‑150 (MQ=60)
                                                                                 AATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAA                                                                   >  SRR19973079.3634459/1‑150 (MQ=60)
                                                                                 AATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAA                                                                   >  SRR19973079.2595009/1‑150 (MQ=60)
                                                                                 AATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAA                                                                   >  SRR19973079.2595099/1‑150 (MQ=60)
                                                                                    GATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTT                                                                >  SRR19973079.3887137/1‑150 (MQ=60)
                                                                                    GATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTT                                                                <  SRR19973079.1797282/150‑1 (MQ=60)
                                                                                    GATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTT                                                                >  SRR19973079.3887287/1‑150 (MQ=60)
                                                                                    GATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTT                                                                <  SRR19973079.1797276/150‑1 (MQ=60)
                                                                                          ACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATC                                                          <  SRR19973079.3875682/150‑1 (MQ=60)
                                                                                           CTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCC                                                         <  SRR19973079.530547/150‑1 (MQ=60)
                                                                                             GAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGC                                                       >  SRR19973079.2144187/1‑150 (MQ=60)
                                                                                               AATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGG                                                     <  SRR19973079.2852009/150‑1 (MQ=60)
                                                                                                   CATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATG                                                 >  SRR19973079.4032157/1‑150 (MQ=60)
                                                                                                    ATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGA                                                >  SRR19973079.128256/1‑150 (MQ=60)
                                                                                                    ATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGA                                                >  SRR19973079.2671051/1‑150 (MQ=60)
                                                                                                    ATCGGCTTCAGTTCGTAATCCAGCTTCAGCTATTTAGCGATGGCAGCACACAGATAAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGA                                                <  SRR19973079.1081309/150‑1 (MQ=60)
                                                                                                    ATCGGATTCAGTTCTTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGA                                                <  SRR19973079.1081659/150‑1 (MQ=60)
                                                                                                       GGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACACCTAATTTTTTATCCGCGGCATGAGAA                                             >  SRR19973079.2925113/1‑150 (MQ=60)
                                                                                                       GGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAA                                             <  SRR19973079.4194465/150‑1 (MQ=60)
                                                                                                           TCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAA                                         <  SRR19973079.1894068/150‑1 (MQ=60)
                                                                                                                    AATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAG                                <  SRR19973079.574792/150‑1 (MQ=60)
                                                                                                                    AATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAG                                <  SRR19973079.2536342/150‑1 (MQ=60)
                                                                                                                     ATCCAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATAAGCGGCATGAGAAGAAACCGCAAAAGC                               >  SRR19973079.2979488/1‑150 (MQ=60)
                                                                                                                        CAGCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACACCTAATTTTTTATCCGCGGCATGAGACGAAACCGCAAAAGCCAG                            <  SRR19973079.2001073/150‑1 (MQ=60)
                                                                                                                          GCTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGG                          >  SRR19973079.2724810/1‑150 (MQ=60)
                                                                                                                           CTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGT                         >  SRR19973079.538707/1‑150 (MQ=60)
                                                                                                                           CTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGT                         <  SRR19973079.4126987/150‑1 (MQ=60)
                                                                                                                           CTTCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGT                         <  SRR19973079.4125485/150‑1 (MQ=60)
                                                                                                                             TCAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCA                       >  SRR19973079.1615789/1‑150 (MQ=60)
                                                                                                                              CAGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAG                      <  SRR19973079.382707/150‑1 (MQ=60)
                                                                                                                               AGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGT                     <  SRR19973079.1381436/150‑1 (MQ=60)
                                                                                                                               AGCTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGT                     <  SRR19973079.4287664/150‑1 (MQ=60)
                                                                                                                                 CTCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGC                   >  SRR19973079.99248/1‑150 (MQ=60)
                                                                                                                                  TCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTTTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCA                  <  SRR19973079.70415/150‑1 (MQ=60)
                                                                                                                                  TCTTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCA                  <  SRR19973079.70438/150‑1 (MQ=60)
                                                                                                                                    TTTAGCGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGC                >  SRR19973079.4224965/1‑150 (MQ=60)
                                                                                                                                         CGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTG           <  SRR19973079.1333858/150‑1 (MQ=60)
                                                                                                                                         CGATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTG           <  SRR19973079.2323328/150‑1 (MQ=60)
                                                                                                                                         CGATGGCAGCACACAGATAAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTG           <  SRR19973079.3095005/150‑1 (MQ=60)
                                                                                                                                          GATGGCAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGA          >  SRR19973079.3736772/1‑150 (MQ=60)
                                                                                                                                               CAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTT     >  SRR19973079.1082756/1‑150 (MQ=60)
                                                                                                                                               CAGCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTT     >  SRR19973079.2813294/1‑150 (MQ=60)
                                                                                                                                                 GCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGACCAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTTT   <  SRR19973079.163571/150‑1 (MQ=60)
                                                                                                                                                 GCACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTTT   <  SRR19973079.163957/150‑1 (MQ=60)
                                                                                                                                                  CACACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTTTA  >  SRR19973079.32460/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                    
GAAATCGATCGCTTTTTTACGCTCGTCGGTGATGGTAATGCCCGCCAGCGCCAGATCGACGTTTTTGGTTTGCAGTGCCGGAATGATCCCACTGAAATCCATCGGCTTCAGTTCGTAATCCAGCTTCAGCTCTTTAGCGATGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTTTA  >  CP009273/843152‑843447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑