Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,386,008 G→T 87.7% Q35K (CAG→AAG)  ycjY ← S9 homolog non‑peptidase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,386,0080GT87.7% 542.1 / 64.8 212Q35K (CAG→AAG) ycjYS9 homolog non‑peptidase family protein
Reads supporting (aligned to +/- strand):  ref base G (15/12);  new base T (54/129);  total (71/141)
Fisher's exact test for biased strand distribution p-value = 1.42e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 212 total aligned reads displayed.

TCTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGAT  >  CP009273/1385860‑1386154
                                                                                                                                                    |                                                                                                                                                  
   aaCTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATAc                                                                                                                                                <  2:3763594/150‑1 (MQ=255)
       gACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCC‑TTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACg                                                                                                                                           <  1:3199763/150‑1 (MQ=255)
           cgGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGt                                                                                                                                        <  2:3370601/150‑1 (MQ=255)
            ggCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGtt                                                                                                                                       <  2:2736871/150‑1 (MQ=255)
            ggCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGtt                                                                                                                                       <  2:3987925/150‑1 (MQ=255)
             gCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGttt                                                                                                                                      <  1:4411534/150‑1 (MQ=255)
             gCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGttt                                                                                                                                      <  2:1174573/150‑1 (MQ=255)
              cTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTtc                                                                                                                                     <  1:1192133/150‑1 (MQ=255)
              cTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTtc                                                                                                                                     <  1:2161019/150‑1 (MQ=255)
                 gccgccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTtcatc                                                                                                                                  <  2:3971738/150‑1 (MQ=255)
                    gccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCCCCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCCTCaaa                                                                                                                               >  2:557987/1‑150 (MQ=255)
                      cACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCAGGGTGGGATAACACACTTGCCGTATACAGACGGGGTTCATCaaaat                                                                                                                             >  2:2814597/1‑148 (MQ=255)
                      cACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAAAGCCCCCACCAGGGTGAGATAACACAAATTCCTTAAACTGACGGGTTTCATCaaaat                                                                                                                             >  2:2291378/1‑148 (MQ=255)
                       aCTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAAttt                                                                                                                            <  2:4296677/150‑1 (MQ=255)
                       aCTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAAttt                                                                                                                            <  2:3022296/150‑1 (MQ=255)
                       aCTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAAttt                                                                                                                            <  2:2901954/150‑1 (MQ=255)
                           tCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGa                                                                                                                        <  1:3977271/150‑1 (MQ=255)
                            cACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAg                                                                                                                       <  2:37891/150‑1 (MQ=255)
                              ccTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAggg                                                                                                                     <  2:4201066/150‑1 (MQ=255)
                              ccTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTTACGCCCCCCCCCCGGGGGGTTAAAACAAATTACGTATAAAGACGGGGTTGATAAAATTTTTGTggg                                                                                                                     >  1:777470/1‑150 (MQ=255)
                                  gATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAAt                                                                                                                 <  2:862391/150‑1 (MQ=255)
                                     aaGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCACCAGGGTGAGATAACACAAATGCCATATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGa                                                                                                              >  2:483466/1‑150 (MQ=255)
                                         tGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTAACCCCCCCACCCGGGGGAGATAACACAAATTCCCTATAATGACGGGGTTCATCAAAATTTGGAGGGGCATAGATCAc                                                                                                          >  1:1431547/1‑150 (MQ=255)
                                         tGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTTTTATTTAAAGCCCCCCCCGGGGTGAGATAACAAAATTTCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATATATAAc                                                                                                          >  1:1431538/1‑150 (MQ=255)
                                           cGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTTAAGCCCCCCCCGGGGTGTGATAACACAATTTCCGTATAAAGACGGGGTTCATCAAAATTTTGAGGGTAATATATCAcag                                                                                                        >  1:635268/1‑148 (MQ=255)
                                             tCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCa                                                                                                      <  2:1833430/150‑1 (MQ=255)
                                             tCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTTAAGCCCCCCCCGGGGTGAGATAACACCATTTCCTGATAAAGAAGGGGTTAATCAAATTTTTGAGGGGAatatataaaagca                                                                                                      >  1:1061381/1‑143 (MQ=255)
                                              cATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAg                                                                                                     <  1:962012/150‑1 (MQ=255)
                                              cATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTTAAGCCCCCACCAGGGGGAGATAACACAAATGGCGTATAATGTCGGGTTTGATCAAATTTTTGTGGGGAATAGATAAcagcag                                                                                                     >  1:3169598/1‑150 (MQ=255)
                                               aTAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAACGCCCCCACCGGGGTGTGATAACACAAATTCCGTATAATGACGGGGTTCATCAAATTTTTGAGGGTAATAGATAACTGCAGa                                                                                                    >  1:1880484/1‑150 (MQ=255)
                                                  aGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTCACGCCCACACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATTGATCACTGCAGACaa                                                                                                 <  2:375544/150‑1 (MQ=255)
                                                       tAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTTAACCCCCCCCCCGGGGGTGGTAACACAAAATCCATATAAAGACGGGGTTGATCAAAATTTTGTGGGGAAAAGATAAAATCACACAAAgaga                                                                                            >  1:4233574/1‑150 (MQ=255)
                                                        aGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGAt                                                                                           <  1:2641708/150‑1 (MQ=255)
                                                           gACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGt                                                                                        <  2:2073757/150‑1 (MQ=255)
                                                            aCAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGtt                                                                                       <  2:2302241/150‑1 (MQ=255)
                                                              aaaTCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTAAAGCCCCCCCCAGGGTGCGATTACACAAATGCCGGATAATGACGGGGTTCATCAAAGTTTGGAGGGTAATATGTCACTGCAGACAAAGTGATGGTggg                                                                                     >  2:59791/1‑150 (MQ=255)
                                                              aaaTCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTgg                                                                                     <  1:3380194/150‑1 (MQ=255)
                                                               aaTCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGa                                                                                    <  2:2690637/150‑1 (MQ=255)
                                                                 tCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCCCCGGGGTGAGATAACACAAATTCCTTATACTGTCGGGGTTCATCAAATTTTGGAGGGAAATATATAACTGCAGACAAAGAGATGGTTGGatt                                                                                  >  2:525892/1‑150 (MQ=255)
                                                                 tCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGatt                                                                                  <  1:1828229/150‑1 (MQ=255)
                                                                  cccTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGatta                                                                                 <  2:89584/150‑1 (MQ=255)
                                                                    cTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGattatt                                                                               <  1:526106/150‑1 (MQ=255)
                                                                    cTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGattatt                                                                               <  2:1682221/150‑1 (MQ=255)
                                                                      tttCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGc                                                                             <  1:301521/150‑1 (MQ=255)
                                                                      tttCCGCCAGTTTTTTGGCATAGGGTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATGGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGc                                                                             <  2:4371196/150‑1 (MQ=255)
                                                                       ttCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCt                                                                            <  1:260475/150‑1 (MQ=255)
                                                                        tCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCtt                                                                           <  1:938827/150‑2 (MQ=255)
                                                                         ccGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGt                                                                          <  1:3334092/150‑1 (MQ=255)
                                                                          cGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGtt                                                                         <  1:345019/150‑1 (MQ=255)
                                                                          cGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCATTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGtt                                                                         <  2:1779360/150‑1 (MQ=255)
                                                                          cGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTATTTAAAGCCCCCACCTTGGTGATAAAACAAAAATGCCTAATACTGACCGGTTTCATCAAATTTTGGAGGGAAAAAGATCACAGCAGACAAAAAGATGGTTGGattatatctttt                                                                         >  2:2441186/1‑143 (MQ=255)
                                                                              aGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTg                                                                     <  2:4339600/150‑1 (MQ=255)
                                                                                    ttGGCATAGGTTCCGGCCGTTTGTTCTTTCACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTg                                                               <  1:1682609/150‑1 (MQ=255)
                                                                                         aTAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACttt                                                          <  1:2742795/150‑1 (MQ=255)
                                                                                         aTAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACttt                                                          <  1:2529196/150‑1 (MQ=255)
                                                                                         aTAGGGTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACttt                                                          <  2:451872/150‑1 (MQ=255)
                                                                                          tAGGTTCCGGCCGTGTGTTCTTTAACCCCCCCACCAGGGTGAGAAAACACCAATGCCATATACTGACGGGTTTCATCAAATTTTGGAGGGAAAAAGAAAACTGCACACAAACAGATGGGTGGATTATTGCTGTTAGTGAAGCTGACGtta                                                         >  2:2143525/1‑150 (MQ=255)
                                                                                           aGGTTCCGGCAGTTTGTTCTTTATCGCCCCCACGTGGGTGAGATAAAAGAATTGCCTTATACTGACGGGTTTCATATTATTTTGGAGGGAAATAGATCTCTGCATACAAAGAGATGGTTTGATTATTGCTTTTAGTGATGCTGACTttat                                                        <  2:4485266/150‑1 (MQ=255)
                                                                                            ggTTCCGGCCGAGTGTTGTTTTACGCCCCCAACTGGGTGAGATAACACAATTGCATTATAGTGACGGGTTTCATCATATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTTGTGAAGCTGACTttatt                                                       <  1:1721630/150‑1 (MQ=255)
                                                                                              ttCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTtattat                                                     <  1:191030/150‑1 (MQ=255)
                                                                                               tCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTattatt                                                    <  1:3463785/150‑1 (MQ=255)
                                                                                                 cGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtca                                                  <  2:388084/150‑1 (MQ=255)
                                                                                                   gCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtcatc                                                <  2:4456511/150‑1 (MQ=255)
                                                                                                     cGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTcatcat                                              >  1:3601255/1‑150 (MQ=255)
                                                                                                        ttGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTcc                                           <  1:1501959/150‑1 (MQ=255)
                                                                                                         tGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCg                                          >  2:3344584/1‑150 (MQ=255)
                                                                                                         tGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCg                                          >  1:4094376/1‑150 (MQ=255)
                                                                                                          gTTCTTTAACGCCCCCACCTTGTTGAGATAACACAATTGCCTTATACTGACGGTTTTCATCAAATTTTGTATGTAAATAGATCACTGAAGACAAAGATATTGTTGGATTATTTCTGTTAGTGATGCTTACTTTATTATTCATCATTCCGt                                         <  2:2996634/150‑1 (MQ=255)
                                                                                                          gTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGt                                         <  2:35180/150‑1 (MQ=255)
                                                                                                           ttCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGtt                                        <  2:2890538/150‑1 (MQ=255)
                                                                                                              tttAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCt                                     <  2:1282864/150‑1 (MQ=255)
                                                                                                               ttAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTc                                    <  1:2062658/150‑1 (MQ=255)
                                                                                                                tAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCa                                   <  2:1601088/150‑1 (MQ=255)
                                                                                                                tAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCa                                   >  2:3447995/1‑150 (MQ=255)
                                                                                                                 aaCGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCAt                                  <  2:59172/150‑1 (MQ=255)
                                                                                                                  aCGCCCCCACCTGGGTTATATAACAAAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATg                                 <  2:4009743/150‑1 (MQ=255)
                                                                                                                      ccccACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGt                             <  2:3854652/150‑1 (MQ=255)
                                                                                                                       cccACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGtt                            <  2:3939668/150‑1 (MQ=255)
                                                                                                                       cccACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGtt                            <  1:1715336/150‑1 (MQ=255)
                                                                                                                       cccACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGtt                            <  1:2866369/150‑1 (MQ=255)
                                                                                                                        ccACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTg                           >  1:1465859/1‑150 (MQ=255)
                                                                                                                          aCCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTc                         <  1:3775889/150‑1 (MQ=255)
                                                                                                                           ccTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCg                        >  1:2796105/1‑150 (MQ=255)
                                                                                                                               ggTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTc                    <  1:2276051/150‑1 (MQ=255)
                                                                                                                               ggTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTc                    <  2:4119535/150‑1 (MQ=255)
                                                                                                                                gTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCg                   <  2:306318/150‑1 (MQ=255)
                                                                                                                                gTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCg                   <  2:3644826/150‑1 (MQ=255)
                                                                                                                                 tGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGt                  >  1:4495313/1‑150 (MQ=255)
                                                                                                                                  gagaTAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGtt                 >  2:3473021/1‑150 (MQ=255)
                                                                                                                                   agaTAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGttt                >  2:1565650/1‑150 (MQ=255)
                                                                                                                                   agaTAACACAAGTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGttt                <  2:625416/150‑1 (MQ=255)
                                                                                                                                    gaTAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGtttt               <  1:605757/150‑1 (MQ=255)
                                                                                                                                    gaTAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGtttt               <  1:2235561/150‑1 (MQ=255)
                                                                                                                                      tAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTaa             >  1:3218509/1‑150 (MQ=255)
                                                                                                                                       aaCACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAAc            >  2:3987107/1‑150 (MQ=255)
                                                                                                                                       aaCACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAAc            <  2:1471490/150‑1 (MQ=255)
                                                                                                                                               tGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTg    <  1:2291378/150‑1 (MQ=255)
                                                                                                                                               tGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTg    <  1:3902424/150‑1 (MQ=255)
                                                                                                                                               tGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTg    <  1:1292307/150‑1 (MQ=255)
                                                                                                                                                 ccTGATACTGACGGGTTTCATCACATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGat  >  1:1246636/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TCTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGAT  >  CP009273/1385860‑1386154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGATATC  >  CP009273/1385860‑1386157
                                                                                                                                                    |                                                                                                                                                     
TCTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTA                                                                                                                                                      <  SRR19973080.2481510/150‑1 (MQ=60)
TCTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTA                                                                                                                                                      <  SRR19973080.2967020/150‑1 (MQ=60)
TCTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGA                                                                                                                                                      <  SRR19973080.2253260/150‑1 (MQ=60)
TCTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGA                                                                                                                                                      <  SRR19973080.2253270/150‑1 (MQ=60)
       GACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCC‑TTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACG                                                                                                                                              <  SRR19973080.3217425/150‑1 (MQ=60)
       GACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCC‑TTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACG                                                                                                                                              <  SRR19973080.3217464/150‑1 (MQ=60)
        ACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACG                                                                                                                                              <  SRR19973080.1882938/150‑1 (MQ=60)
        ACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACG                                                                                                                                              <  SRR19973080.4169180/150‑1 (MQ=60)
        ACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACG                                                                                                                                              <  SRR19973080.3453791/150‑1 (MQ=60)
             GCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTT                                                                                                                                         <  SRR19973080.4435528/150‑1 (MQ=60)
              CTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTC                                                                                                                                        <  SRR19973080.2173098/150‑1 (MQ=60)
              CTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTC                                                                                                                                        <  SRR19973080.1199308/150‑1 (MQ=60)
                  CCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCA                                                                                                                                    <  SRR19973080.1215930/150‑1 (MQ=60)
                           TCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGA                                                                                                                           <  SRR19973080.1569019/150‑1 (MQ=60)
                           TCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGA                                                                                                                           <  SRR19973080.3999160/150‑1 (MQ=60)
                                TTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAA                                                                                                                      <  SRR19973080.687217/150‑1 (MQ=60)
                                 TGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAA                                                                                                                     <  SRR19973080.3726676/150‑1 (MQ=60)
                                    TAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAG                                                                                                                  <  SRR19973080.854807/150‑1 (MQ=60)
                                      AGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGAT                                                                                                                <  SRR19973080.2863712/150‑1 (MQ=60)
                                        ATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATTGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCA                                                                                                              <  SRR19973080.3522516/150‑1 (MQ=60)
                                         TGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTTTTATTTAACGCCCCCACCAGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGTGGGAAATAGATAAC                                                                                                             >  SRR19973080.1439925/1‑150 (MQ=60)
                                         TGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTAACCCCCCCACCCGGGGGAGATAACACAAATTCCCTATAATGACGGGGTTCATCAAAATTTGGAGGGGCATAGATCAC                                                                                                             >  SRR19973080.1439926/1‑150 (MQ=60)
                                         TGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCAC                                                                                                             <  SRR19973080.2581133/150‑1 (MQ=60)
                                         TGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTTTTATTTAAAGCCCCCCCCGGGGTGAGATAACAAAATTTCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATATATAAC                                                                                                             >  SRR19973080.1439917/1‑150 (MQ=60)
                                             TCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCA                                                                                                         <  SRR19973080.1109729/150‑1 (MQ=60)
                                             TCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTTACGCCCCCACCAGGGTGAGATAAAACAATTGCCTGATACAGACGGGGTTCATAAAATTTTTGTGGGAAAAAGATAACAGCA                                                                                                         >  SRR19973080.3359572/1‑150 (MQ=60)
                                             TCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTTAAGCCCCCCCCGGGGTGAGATAACACCATTTCCTGATAaagaaggggttaatcaaatttttgaggggaatatataaaagca                                                                                                         >  SRR19973080.1067589/1‑107 (MQ=60)
                                              CATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAG                                                                                                        <  SRR19973080.3840864/150‑1 (MQ=60)
                                              CATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAG                                                                                                        <  SRR19973080.967843/150‑1 (MQ=60)
                                              CATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAG                                                                                                        <  SRR19973080.967410/150‑1 (MQ=60)
                                              CATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAG                                                                                                        <  SRR19973080.1892524/150‑1 (MQ=60)
                                               ATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAACGCCCCCACCGGGGTGTGATAACACAAATTCCGTATAATGACGGGGTTCATCAAATTTTTGAGGGTAATAGATAACTGCAGA                                                                                                       >  SRR19973080.1890950/1‑150 (MQ=60)
                                                   GCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAA                                                                                                   <  SRR19973080.1816177/150‑1 (MQ=60)
                                                        AGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGAT                                                                                              <  SRR19973080.2655961/150‑1 (MQ=60)
                                                              AAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGG                                                                                        <  SRR19973080.3398486/150‑1 (MQ=60)
                                                                 TCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATT                                                                                     <  SRR19973080.1838552/150‑1 (MQ=60)
                                                                    CTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATT                                                                                  <  SRR19973080.529454/150‑1 (MQ=60)
                                                                    CTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATT                                                                                  <  SRR19973080.3677366/150‑1 (MQ=60)
                                                                    CTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATT                                                                                  <  SRR19973080.532581/150‑1 (MQ=60)
                                                                      TTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGC                                                                                <  SRR19973080.303443/150‑1 (MQ=60)
                                                                       TTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCT                                                                               <  SRR19973080.262227/150‑1 (MQ=60)
                                                                        TCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTT                                                                              <  SRR19973080.944562/150‑1 (MQ=60)
                                                                         CCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGT                                                                             <  SRR19973080.3352258/150‑1 (MQ=60)
                                                                         CCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGT                                                                             <  SRR19973080.3351946/150‑1 (MQ=60)
                                                                          CGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTT                                                                            <  SRR19973080.347064/150‑1 (MQ=60)
                                                                          CGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTT                                                                            <  SRR19973080.588706/150‑1 (MQ=60)
                                                                                    TTGGCATAGGTTCCGGCCGTTTGTTCTTTCACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTG                                                                  <  SRR19973080.1692421/150‑1 (MQ=60)
                                                                                       GCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACT                                                               <  SRR19973080.2903960/150‑1 (MQ=60)
                                                                                       GCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACT                                                               <  SRR19973080.3062958/150‑1 (MQ=60)
                                                                                       GCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACT                                                               <  SRR19973080.2903885/150‑1 (MQ=60)
                                                                                        CATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTT                                                              <  SRR19973080.3957894/150‑1 (MQ=60)
                                                                                         ATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTT                                                             <  SRR19973080.2757896/150‑1 (MQ=60)
                                                                                         ATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTT                                                             <  SRR19973080.2543097/150‑1 (MQ=60)
                                                                                            GGTTCCGGCCGAGTGTTGTTTTACGCCCCCAACTGGGTGAGATAACACAATTGCATTATAGTGACGGGTTTCATCATATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTTGTGAAGCTGACTTTATT                                                          <  SRR19973080.1731598/150‑1 (MQ=60)
                                                                                              TTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTAT                                                        <  SRR19973080.192485/150‑1 (MQ=60)
                                                                                              TTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTAT                                                        <  SRR19973080.474542/150‑1 (MQ=60)
                                                                                               TCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATT                                                       <  SRR19973080.3482781/150‑1 (MQ=60)
                                                                                               TCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATT                                                       <  SRR19973080.3482359/150‑1 (MQ=60)
                                                                                                    CCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCA                                                  >  SRR19973080.2015759/1‑150 (MQ=60)
                                                                                                    CCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCA                                                  >  SRR19973080.4302645/1‑150 (MQ=60)
                                                                                                     CGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCAT                                                 >  SRR19973080.3621160/1‑150 (MQ=60)
                                                                                                        TTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCC                                              <  SRR19973080.1510506/150‑1 (MQ=60)
                                                                                                         TGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCG                                             >  SRR19973080.4116649/1‑150 (MQ=60)
                                                                                                               TTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTC                                       <  SRR19973080.2074354/150‑1 (MQ=60)
                                                                                                                  ACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTACATAAAATTTTGGAGGTAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATG                                    >  SRR19973080.633920/1‑150 (MQ=60)
                                                                                                                       CCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTT                               <  SRR19973080.1725281/150‑1 (MQ=60)
                                                                                                                       CCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTT                               <  SRR19973080.2882074/150‑1 (MQ=60)
                                                                                                                        CCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTG                              >  SRR19973080.1473212/1‑150 (MQ=60)
                                                                                                                        CCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTG                              >  SRR19973080.1474311/1‑150 (MQ=60)
                                                                                                                          ACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTC                            <  SRR19973080.1498857/150‑1 (MQ=60)
                                                                                                                          ACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTC                            <  SRR19973080.3796417/150‑1 (MQ=60)
                                                                                                                           CCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCG                           <  SRR19973080.4460428/150‑1 (MQ=60)
                                                                                                                           CCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCG                           >  SRR19973080.2811518/1‑150 (MQ=60)
                                                                                                                           CCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCG                           >  SRR19973080.2811466/1‑150 (MQ=60)
                                                                                                                               GGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTC                       >  SRR19973080.2677755/1‑150 (MQ=60)
                                                                                                                               GGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTC                       <  SRR19973080.2288513/150‑1 (MQ=60)
                                                                                                                               GGTGAGATAACACAAGTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTC                       <  SRR19973080.2288562/150‑1 (MQ=60)
                                                                                                                                 TGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGT                     >  SRR19973080.4519602/1‑150 (MQ=60)
                                                                                                                                   AGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTT                   >  SRR19973080.2488015/1‑150 (MQ=60)
                                                                                                                                    GATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTT                  <  SRR19973080.2247852/150‑1 (MQ=60)
                                                                                                                                    GATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTT                  <  SRR19973080.609505/150‑1 (MQ=60)
                                                                                                                                    GATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTT                  <  SRR19973080.2247822/150‑1 (MQ=60)
                                                                                                                                      TAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAA                >  SRR19973080.3236260/1‑150 (MQ=60)
                                                                                                                                        ACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACG              <  SRR19973080.4052722/150‑1 (MQ=60)
                                                                                                                                               TGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTG       <  SRR19973080.3923964/150‑1 (MQ=60)
                                                                                                                                               TGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTG       >  SRR19973080.2620444/1‑150 (MQ=60)
                                                                                                                                               TGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTG       <  SRR19973080.2303996/150‑1 (MQ=60)
                                                                                                                                               TGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTG       <  SRR19973080.1300124/150‑1 (MQ=60)
                                                                                                                                               TGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTG       <  SRR19973080.1300041/150‑1 (MQ=60)
                                                                                                                                                GCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGGTGTCGGTTCGTTTTAACGGTTGGTGA      >  SRR19973080.1396528/1‑150 (MQ=60)
                                                                                                                                                 CCTGATACTGACGGGTTTCATCACATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGAT     >  SRR19973080.1254185/1‑150 (MQ=60)
                                                                                                                                                   TTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGATAT   <  SRR19973080.1804865/150‑1 (MQ=60)
                                                                                                                                                    TATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGATATC  >  SRR19973080.1594833/1‑150 (MQ=60)
                                                                                                                                                    TATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGATATC  <  SRR19973080.629969/150‑1 (MQ=60)
                                                                                                                                                    TATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGATATC  <  SRR19973080.629986/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
TCTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGATATC  >  CP009273/1385860‑1386157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑