Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,386,008 G→T 100% Q35K (CAG→AAG)  ycjY ← S9 homolog non‑peptidase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,386,0080GT98.4% 738.5 / 6.6 245coding (103/921 nt)ycjYS9 homolog non‑peptidase family protein
Reads supporting (aligned to +/- strand):  ref base G (2/0);  major base T (94/145);  minor base . (4/0);  total (100/145)
Fisher's exact test for biased strand distribution p-value = 2.55e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 251 total aligned reads displayed.

AACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTG  >  CP009273/1385863‑1386152
                                                                                                                                                   |                                                                                                                                                
aaCTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTAt                                                                                                                                                <  1:356687/148‑1 (MQ=255)
 aCTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTtata                                                                                                                                               <  1:1205567/148‑1 (MQ=255)
 aCTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTtata                                                                                                                                               >  2:3476018/1‑148 (MQ=255)
 aCTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTtata                                                                                                                                               <  1:2796829/148‑1 (MQ=255)
  cTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATAc                                                                                                                                              <  1:3584411/148‑1 (MQ=255)
  cTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑CATTGCCTtataa                                                                                                                                              >  2:2700399/1‑147 (MQ=255)
    gACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AAATGCCTTATACTg                                                                                                                                            >  2:1073527/1‑148 (MQ=255)
     aCGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGa                                                                                                                                           <  2:2632305/148‑1 (MQ=255)
     aCGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGa                                                                                                                                           <  2:3732848/148‑1 (MQ=255)
      cgcgGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTTAAGCCCCCCCCCGGGGGTGTTAACA‑C‑AAATTCCGGATACTGAc                                                                                                                                          >  1:1020498/1‑148 (MQ=255)
          gCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGt                                                                                                                                      <  1:1212895/148‑1 (MQ=255)
          gCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGt                                                                                                                                      <  2:2781053/148‑1 (MQ=255)
          gCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGt                                                                                                                                      <  2:3170689/148‑1 (MQ=255)
             cgccgcCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAAAGCCCCCACCAGGGTGAGATAACA‑C‑AAATTCCATATAATGACGGGGTtc                                                                                                                                   >  2:336188/1‑148 (MQ=255)
              gccgccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTtca                                                                                                                                  <  2:2423816/148‑1 (MQ=255)
              gccgccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTtca                                                                                                                                  <  2:1646296/148‑1 (MQ=255)
              gccgccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTtca                                                                                                                                  <  1:2210806/148‑1 (MQ=255)
                  ccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCaa                                                                                                                              <  1:2446629/148‑1 (MQ=255)
                  ccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCaa                                                                                                                              <  2:756368/148‑1 (MQ=255)
                     cTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAAAGCCCCCCCCAGGGGGAGATAAAA‑C‑AAATTGCGGATACTGAAGGGTTTAATaaaaat                                                                                                                           >  1:2309048/1‑146 (MQ=255)
                      tttCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAAAGCCCCCACCAGGGTGAGATAAAA‑C‑AATTGCCTTATACAGACGGGTTTCATCAAAatt                                                                                                                          >  1:1900326/1‑148 (MQ=255)
                       ttCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAAAGCCCCCAACAGGGTGAGATAACA‑C‑AAATGCCATATACAGACGGGTTTCATCAAAtttt                                                                                                                         >  2:767591/1‑148 (MQ=255)
                         cACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTgg                                                                                                                       <  2:498328/148‑1 (MQ=255)
                         cACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTgg                                                                                                                       <  2:218133/148‑1 (MQ=255)
                         cACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTgg                                                                                                                       <  2:2146528/148‑1 (MQ=255)
                          aCCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGa                                                                                                                      <  1:103143/148‑1 (MQ=255)
                          aCCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGa                                                                                                                      <  1:3504249/148‑1 (MQ=255)
                              tGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTTTTTAACGCCCCCCCCGGGGTGGGCTAAAA‑C‑AAATTCCTTATAATGACGGGGTTGATCAAATTTTTGTgggc                                                                                                                  >  1:3387511/1‑147 (MQ=255)
                              tGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTTAACCCCCCCCCAGGGTGAGATAACA‑C‑AAATGCCGTATAAAGCCGGGTTTAAACAAAATTTTGTGGGa                                                                                                                  >  1:216081/1‑148 (MQ=255)
                               gATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGaa                                                                                                                 <  1:137627/148‑1 (MQ=255)
                                  aaGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTAACGCCCCCACCTGGGTGTGATAACA‑C‑AATTGCCGTATACTGACGGGGTTCATCAAATTTTTGTGGGAAATa                                                                                                              >  2:860569/1‑148 (MQ=255)
                                    gATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGa                                                                                                            <  2:2988935/148‑1 (MQ=255)
                                     aTGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTTAAGCCCCCCCCGGGGGGAGATTAAA‑C‑AAGTTCCTTATAATGTCGGGTTTGATGAAATTTTGTGGGGGAatatat                                                                                                           >  1:423254/1‑148 (MQ=255)
                                       gCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCa                                                                                                         <  2:2818551/148‑1 (MQ=255)
                                         gTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACt                                                                                                       <  2:1439778/148‑1 (MQ=255)
                                          tCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCAGGGTGAGATAACC‑C‑AATTGCCTTATACGGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTg                                                                                                      >  2:533195/1‑148 (MQ=255)
                                              aaGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGa                                                                                                  <  2:240368/148‑1 (MQ=255)
                                              aaGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTTTTGGTTAAAGCCCCCCCCCGGGGGGGTTAACACC‑AATTGCGT‑ATAAAGAACGGTTTGATAAAAAATTTGGGGGGAATAGATCaaagcaca                                                                                                  >  1:1770283/1‑141 (MQ=255)
                                                   aTAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAag                                                                                             <  1:1776100/148‑1 (MQ=255)
                                                    tAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACCCCCCCACCGGGGTGAGATAACA‑C‑AAATTCCGTATAATGACGGGGTTCATCAAATTTTGGAGGGAAAAACATCACAGCAGAAAAaga                                                                                            >  2:635415/1‑148 (MQ=255)
                                                     aGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAagag                                                                                           <  1:1662544/148‑1 (MQ=255)
                                                     aGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAagag                                                                                           <  2:2227815/148‑1 (MQ=255)
                                                     aGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAagag                                                                                           <  2:300067/148‑1 (MQ=255)
                                                     aGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAAACCCCCCCCCCGGGGGGGCTAACA‑CAAATTGCGT‑ATAATTACGGGTTTGATCAAAATTTTGAGGGACATAGATCAATGCAGACAAagac                                                                                           >  1:2054059/1‑147 (MQ=255)
                                                     aGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAACGCCCCCACCGGGGTGTGATAACA‑C‑AATTTCCGTATACAGACGGGTTTAATCAAATTTTTGTGGGAAAAAGATCACAGCAGACAAagag                                                                                           >  1:2540588/1‑148 (MQ=255)
                                                      gTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAgaga                                                                                          <  1:1884751/148‑1 (MQ=255)
                                                      gTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAgaga                                                                                          <  2:403061/148‑1 (MQ=255)
                                                      gTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAgaga                                                                                          <  2:1633740/148‑1 (MQ=255)
                                                         aCAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATgg                                                                                       <  1:885831/148‑1 (MQ=255)
                                                         aCAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATgg                                                                                       <  2:1241996/148‑1 (MQ=255)
                                                         aCAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATgg                                                                                       <  2:1914121/148‑1 (MQ=255)
                                                         aCAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATgg                                                                                       <  1:1359863/148‑1 (MQ=255)
                                                         aCAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATgg                                                                                       <  1:3390465/148‑1 (MQ=255)
                                                             aTCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTgg                                                                                   <  2:3782598/148‑1 (MQ=255)
                                                                 cTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGatta                                                                               <  1:3185571/148‑1 (MQ=255)
                                                                  ttttCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTTTTGTTTAAAGCCCCCACCAGGGGGATATAAAA‑C‑AATTTCCTTATAAAGACGGGTTTAATAAAAAATTTGTGGGAAATAAATAACAGGAGACAAAGACAATGTTTGatttt                                                                              >  1:3108671/1‑146 (MQ=255)
                                                                       cGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCCCCGGGGTGAGATAACA‑C‑AATTGCCGTATAATGAAGGGTGTCATCAAAATTTTGAGGGAAATAGATCAATGCAGACCAAGAGATGGTGGGAGTATTGCTg                                                                         >  2:2690301/1‑148 (MQ=255)
                                                                        gCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTAACCCCCCCACCGGGGTGAGATAAAA‑C‑AAATGCCGTATACTGACGGGTTTCATCAAATTGTGGTGGGAAATAGATCACTGCAGACAAAGAGATGGGTTGATTATTGCTGt                                                                        >  2:3374034/1‑148 (MQ=255)
                                                                          cAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTAACGCCCCCACCAGGGGGAGATAACA‑A‑AATTGCCTTATAAAGACGGGTTTCATCAAAATTTGGAGGGAAATAGATCACAGCAGACAAAGAGATGGTTGGATTATTGCGGTTa                                                                      >  2:1597839/1‑148 (MQ=255)
                                                                              tttttGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCAGGGTGAGATAACA‑C‑AATTGCCTTATACAGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACCAAGAGATGGTTGGATTATTGCTGTTAGTGa                                                                  >  2:2529709/1‑148 (MQ=255)
                                                                              tttttGGCATAGGTTCCGGCCGTTTGTTCTTTAACCCCCCCACCAGGGTGAGATAAAA‑A‑AAATGCCTTATAATGACGGGTTTCATAAAATTTTTGAGGGAAATATATCACAGCAGACAAAGAGATGGTTGGATTATTTCTTTTattta                                                                  >  2:833292/1‑144 (MQ=255)
                                                                               ttttGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGaa                                                                 <  1:630557/148‑1 (MQ=255)
                                                                                 ttGGCATAGGTTCCGGCCGTTTGTTCTTTAACCCCCCCACCAGGGTGAGATAAAA‑C‑AAATGCCTTATAATGAAGGGTTTCATAAAATATTTGAGGGAAATAGATCACAGCAGACAAATAGATGGTTGGATTATTTCTGTTAGTGAAGc                                                               >  2:1530056/1‑148 (MQ=255)
                                                                                   ggCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTg                                                             <  2:1421600/148‑1 (MQ=255)
                                                                                     cATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCACCTGGGTGAGATAACA‑C‑AAATTCCTTATAAAGACGGGTTTCATCAAATTTTTGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGGTAGTGAAGCttaa                                                           >  2:2528186/1‑145 (MQ=255)
                                                                                      aTAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACt                                                          <  2:274910/148‑1 (MQ=255)
                                                                                      aTAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACt                                                          <  2:2757348/148‑1 (MQ=255)
                                                                                      aTAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACt                                                          <  2:2173873/148‑1 (MQ=255)
                                                                                            tCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTttatta                                                    <  2:943694/148‑1 (MQ=255)
                                                                                            tCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTttatta                                                    <  1:1577628/148‑1 (MQ=255)
                                                                                            tCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTttatta                                                    <  1:3476018/148‑1 (MQ=255)
                                                                                               ggCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtc                                                 <  1:780559/148‑1 (MQ=255)
                                                                                               ggCCGATTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtc                                                 <  2:3415662/148‑1 (MQ=255)
                                                                                                  cGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtcatc                                              >  1:2293437/1‑148 (MQ=255)
                                                                                                    tttGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTcatcat                                            >  1:2999435/1‑148 (MQ=255)
                                                                                                     ttGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCAtt                                           >  1:915544/1‑148 (MQ=255)
                                                                                                         tCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGt                                       >  2:2039056/1‑148 (MQ=255)
                                                                                                            ttAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTcc                                    >  1:2685037/1‑148 (MQ=255)
                                                                                                            ttAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTcc                                    >  1:669163/1‑148 (MQ=255)
                                                                                                                cGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCAt                                <  1:2815339/148‑1 (MQ=255)
                                                                                                                    cccACCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCAGTCCGTTCCTCATGGAg                            <  2:216284/148‑1 (MQ=255)
                                                                                                                       aCCTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTg                         <  2:3068935/148‑1 (MQ=255)
                                                                                                                        ccTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGt                        <  2:2205495/148‑1 (MQ=255)
                                                                                                                        ccTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGt                        >  2:1123064/1‑148 (MQ=255)
                                                                                                                        ccTGGGTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGt                        >  2:2931415/1‑148 (MQ=255)
                                                                                                                           gggTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCgg                     <  1:336188/148‑1 (MQ=255)
                                                                                                                           gggTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCgg                     >  2:1262901/1‑148 (MQ=255)
                                                                                                                           gggTGAGATAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCgg                     <  2:3537356/148‑1 (MQ=255)
                                                                                                                               gagaTAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCg                 <  2:3231218/148‑1 (MQ=255)
                                                                                                                                  aTAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGttt              <  1:3719047/148‑1 (MQ=255)
                                                                                                                                  aTAACA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGttt              >  2:1696123/1‑148 (MQ=255)
                                                                                                                                    aaCA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTa            <  2:1411561/148‑1 (MQ=255)
                                                                                                                                    aaCA‑C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTa            <  1:860569/148‑1 (MQ=255)
                                                                                                                                     aca‑c‑AATTGCCTTAAACTGACGGGATTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGAGTATTGCTGTTAGTGAAGCTGACTTGATTATTCATCATTCCGTTCCTCATGGAGGTGTCGGTTCGGTTTaa           >  2:1800245/1‑148 (MQ=255)
                                                                                                                                             ttGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACGATATAATACATCATTCCGTTCCTCCCCGAGTTGTCGGTTCGTTTTAACGGTTg     <  1:3639454/148‑1 (MQ=255)
                                                                                                                                              tGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTgg    >  1:3786594/1‑148 (MQ=255)
                                                                                                                                              tGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTgg    <  1:3258135/148‑1 (MQ=255)
                                                                                                                                              tGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTgg    >  1:3053449/1‑148 (MQ=255)
                                                                                                                                               gCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGt   <  1:3066985/148‑1 (MQ=255)
                                                                                                                                                ccTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTg  <  1:29036/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
AACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACA‑C‑AATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTG  >  CP009273/1385863‑1386152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGATAT  >  CP009273/1385859‑1386156
                                                                                                                                                      |                                                                                                                                                    
TTCTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATTAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTT                                                                                                                                                      <  SRR19973069.3431297/150‑1 (MQ=60)
TTCTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTT                                                                                                                                                      <  SRR19973069.171408/150‑1 (MQ=60)
TTCTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTT                                                                                                                                                      <  SRR19973069.2113240/150‑1 (MQ=60)
   TAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATA                                                                                                                                                   <  SRR19973069.1453641/150‑1 (MQ=60)
    AACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATAC                                                                                                                                                  <  SRR19973069.359424/150‑1 (MQ=60)
    AACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATAC                                                                                                                                                  <  SRR19973069.359242/150‑1 (MQ=60)
     ACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACT                                                                                                                                                 <  SRR19973069.2815412/150‑1 (MQ=60)
     ACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACT                                                                                                                                                 <  SRR19973069.1213852/150‑1 (MQ=60)
      CTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTG                                                                                                                                                <  SRR19973069.3607789/150‑1 (MQ=60)
         ACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACG                                                                                                                                             <  SRR19973069.890724/150‑1 (MQ=60)
         ACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACG                                                                                                                                             <  SRR19973069.892687/150‑1 (MQ=60)
             GGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTT                                                                                                                                         <  SRR19973069.1252380/150‑1 (MQ=60)
             GGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCAGGGTGTGATAACAC‑AATTGCCTTATACTGACGGGTT                                                                                                                                         >  SRR19973069.2208903/1‑150 (MQ=60)
             GGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAAAGCCCCCACCGGGGTGAGATAACAC‑AAATGCCTTATACTGACGGGTT                                                                                                                                         >  SRR19973069.3488703/1‑150 (MQ=60)
              GCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTT                                                                                                                                        <  SRR19973069.1221216/150‑1 (MQ=60)
                  GCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATC                                                                                                                                    <  SRR19973069.2225786/150‑1 (MQ=60)
                   CCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCA                                                                                                                                   <  SRR19973069.2696627/150‑1 (MQ=60)
                      CCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAAT                                                                                                                                <  SRR19973069.2464164/150‑1 (MQ=60)
                      CCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAAT                                                                                                                                <  SRR19973069.2463100/150‑1 (MQ=60)
                      CCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAAT                                                                                                                                <  SRR19973069.3160509/150‑1 (MQ=60)
                        ACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTATTTAAAGCCCCCACCAGGGTGAGATAAAAC‑AAATGCCTTATAATGACGGGTTTAATCAAATTT                                                                                                                              >  SRR19973069.1913447/1‑150 (MQ=60)
                        ACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAAAGCCCCCACCAGGGTGAGATAAAAC‑AATTGCCTTATACAGACGGGTTTCATCAAAATT                                                                                                                              >  SRR19973069.1913469/1‑150 (MQ=60)
                              ACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGG                                                                                                                        <  SRR19973069.3526807/150‑1 (MQ=60)
                              ACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGG                                                                                                                        <  SRR19973069.104170/150‑1 (MQ=60)
                                CTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTTAAGCCCCCCCCGGGGTGTGATAACAC‑AAATGCCTTATAATGACGGGTTTGATCAAATTTTTGAGGGA                                                                                                                      >  SRR19973069.3671375/1‑150 (MQ=60)
                                CTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTAACGCCCCCCCCGGGGGGAGATAAAACAAATTGCGT‑ATAATGGCGGGTTTCATCAAAATTTTGAGGGA                                                                                                                      >  SRR19973069.217974/1‑150 (MQ=60)
                                CTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGA                                                                                                                      <  SRR19973069.3563797/150‑1 (MQ=60)
                                  TGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAA                                                                                                                    <  SRR19973069.2868022/150‑1 (MQ=60)
                                   GATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAAT                                                                                                                   <  SRR19973069.138898/150‑1 (MQ=60)
                                      AAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTTACGCCCCCCCCGGGGTGAGATAAAAC‑AATTGGCGTATACTGAAGGGTTTGATCAAATTTTtgtggggaatata                                                                                                                >  SRR19973069.821047/1‑137 (MQ=60)
                                       AGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGAT                                                                                                               <  SRR19973069.3012217/150‑1 (MQ=60)
                                         ATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAAAGCCCCCACCGGGGTGTGATAACAC‑AAATGCCGTATACAGACGGGTTTAATCAAAattttgtgggcaatagataa                                                                                                             >  SRR19973069.3809230/1‑130 (MQ=60)
                                               CATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAAAGCCCCCACCCGGGGGGGGTAACAC‑AAATTCCTTATACTGACGGGGTTGATCAAATatttgtgggtaaaagataacagcag                                                                                                       >  SRR19973069.3658170/1‑125 (MQ=60)
                                                       ATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTTTTGTTTAAAGCCCCCACCAGGGTGAGATAACAC‑AAATGCCTTATAATGACGGGGTTCAACAAATTTTGGAGGGAAAAAGATAACAGCAGACAAAGAG                                                                                               >  SRR19973069.2557273/1‑150 (MQ=60)
                                                       ATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAG                                                                                               <  SRR19973069.1788608/150‑1 (MQ=60)
                                                       ATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAACGCCCCCACCGGGGTGTGATAACAC‑AATTTCCGTATACAGACGGGTTTAATCAAATTTTTGTGGGAAAAAGATCACAGCAGACAAAGAG                                                                                               >  SRR19973069.2557400/1‑150 (MQ=60)
                                                         AGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGAT                                                                                             <  SRR19973069.1754271/150‑1 (MQ=60)
                                                         AGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGAT                                                                                             <  SRR19973069.1674064/150‑1 (MQ=60)
                                                          GTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATG                                                                                            <  SRR19973069.1897837/150‑1 (MQ=60)
                                                          GTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATG                                                                                            <  SRR19973069.1625090/150‑1 (MQ=60)
                                                             ACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTT                                                                                         <  SRR19973069.1369332/150‑1 (MQ=60)
                                                             ACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTT                                                                                         <  SRR19973069.3412611/150‑1 (MQ=60)
                                                             ACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTT                                                                                         <  SRR19973069.1369313/150‑1 (MQ=60)
                                                             ACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTT                                                                                         <  SRR19973069.3412388/150‑1 (MQ=60)
                                                             ACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTT                                                                                         <  SRR19973069.3284971/150‑1 (MQ=60)
                                                             ACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTT                                                                                         <  SRR19973069.891955/150‑1 (MQ=60)
                                                                 ATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAAAGCCCCCACCGGGGTGTGATAAAAC‑AAATTGCTTATAATGAAGGGTTTCATCAAAATTTTGAGGGGAATATATCACAGCAGACAAAaatatgggtggtt                                                                                     >  SRR19973069.861990/1‑137 (MQ=43)
                                                                 ATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAAAGCCCCCCCCGGGGGGAGATAACAC‑AAATTCCTTATACAGACGGGTTTGAACAAATTTTTGAGGGtaaaatataaaagcagaaaaaaagattgttggtt                                                                                     >  SRR19973069.306102/1‑116 (MQ=57)
                                                                   CCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTA                                                                                   <  SRR19973069.2277258/150‑1 (MQ=60)
                                                                   CCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTTTTGTTTAAAGCCCCCACCGGGGGGGGATAACAC‑AAATGCCGTATAATGACCGGTTTGATCAAATTTTTGAGGGAAATAGATAACTGCAGACAAAGAtaggggttgggta                                                                                   >  SRR19973069.236784/1‑137 (MQ=60)
                                                                    CCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTAT                                                                                  <  SRR19973069.585388/150‑1 (MQ=60)
                                                                     CTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATT                                                                                 <  SRR19973069.3206271/150‑1 (MQ=60)
                                                                              CAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGT                                                                        <  SRR19973069.3086941/150‑1 (MQ=60)
                                                                                   TTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGC                                                                   <  SRR19973069.634725/150‑1 (MQ=60)
                                                                                          ATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTT                                                            <  SRR19973069.356910/150‑1 (MQ=60)
                                                                                            AGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTAT                                                          <  SRR19973069.1212485/150‑1 (MQ=60)
                                                                                            AGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTAT                                                          <  SRR19973069.1212691/150‑1 (MQ=60)
                                                                                             GGTTCCTGCCGTTTGTTCTTTAACGACCCCACCTGGGTGATATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGATGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATT                                                         <  SRR19973069.2099970/150‑1 (MQ=60)
                                                                                             GGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATT                                                         <  SRR19973069.1897704/150‑1 (MQ=60)
                                                                                             GGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATT                                                         <  SRR19973069.639601/150‑1 (MQ=60)
                                                                                                TCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATT                                                      <  SRR19973069.3498488/150‑1 (MQ=60)
                                                                                                TCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATT                                                      <  SRR19973069.3498487/150‑1 (MQ=60)
                                                                                                TCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATT                                                      <  SRR19973069.1588343/150‑1 (MQ=60)
                                                                                                   GGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCAT                                                   <  SRR19973069.786093/150‑1 (MQ=60)
                                                                                                   GGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCAT                                                   <  SRR19973069.2795312/150‑1 (MQ=60)
                                                                                                    GCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATC                                                  >  SRR19973069.2309021/1‑150 (MQ=60)
                                                                                                    GCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTAATC                                                  >  SRR19973069.2309074/1‑150 (MQ=60)
                                                                                                     CCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCA                                                 <  SRR19973069.1029023/150‑1 (MQ=60)
                                                                                                      CGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCAT                                                <  SRR19973069.3636090/150‑1 (MQ=60)
                                                                                                      CGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCAT                                                >  SRR19973069.3019340/1‑150 (MQ=60)
                                                                                                       GTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATT                                               >  SRR19973069.921794/1‑150 (MQ=60)
                                                                                                       GTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATT                                               >  SRR19973069.510477/1‑150 (MQ=60)
                                                                                                           GTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGT                                           >  SRR19973069.3252354/1‑150 (MQ=60)
                                                                                                            TTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTT                                          <  SRR19973069.943436/150‑1 (MQ=60)
                                                                                                              CTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCC                                        >  SRR19973069.2703030/1‑150 (MQ=60)
                                                                                                              CTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCC                                        >  SRR19973069.673739/1‑150 (MQ=60)
                                                                                                              CTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCC                                        >  SRR19973069.1710706/1‑150 (MQ=60)
                                                                                                                 TAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCA                                     <  SRR19973069.882407/150‑1 (MQ=60)
                                                                                                                 TAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCA                                     >  SRR19973069.199456/1‑150 (MQ=60)
                                                                                                                   ACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATG                                   >  SRR19973069.3245659/1‑150 (MQ=60)
                                                                                                                    CGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGG                                  <  SRR19973069.2833982/150‑1 (MQ=60)
                                                                                                                         CCACCTGGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTG                             <  SRR19973069.3057198/150‑1 (MQ=60)
                                                                                                                               GGGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTT                       <  SRR19973069.338529/150‑1 (MQ=60)
                                                                                                                                GGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTC                      <  SRR19973069.3391064/150‑1 (MQ=60)
                                                                                                                                GGTGAGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTC                      <  SRR19973069.3123594/150‑1 (MQ=60)
                                                                                                                                    AGATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTT                  >  SRR19973069.2863606/1‑150 (MQ=60)
                                                                                                                                     GATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTT                 <  SRR19973069.3832922/150‑1 (MQ=60)
                                                                                                                                      ATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTA                <  SRR19973069.3743371/150‑1 (MQ=60)
                                                                                                                                      ATAACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTA                <  SRR19973069.3743327/150‑1 (MQ=60)
                                                                                                                                        AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAAC              <  SRR19973069.866582/150‑1 (MQ=60)
                                                                                                                                              AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTG         <  SRR19973069.2328274/150‑1 (MQ=60)
                                                                                                                                              AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTG         >  SRR19973069.1217610/1‑150 (MQ=60)
                                                                                                                                               ATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGG        >  SRR19973069.3811158/1‑150 (MQ=60)
                                                                                                                                               ATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGG        >  SRR19973069.3073655/1‑150 (MQ=60)
                                                                                                                                                TTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACGATATAATACATCATTCCGTTCCTCCCCGAGTTGTCGGTTCGTTTTAACGGTTGGT       <  SRR19973069.3663297/150‑1 (MQ=60)
                                                                                                                                                 TGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTG      <  SRR19973069.3279474/150‑1 (MQ=60)
                                                                                                                                                  GCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGA     >  SRR19973069.3319488/1‑150 (MQ=60)
                                                                                                                                                  GCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGA     <  SRR19973069.3087256/150‑1 (MQ=60)
                                                                                                                                                   CCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGAT    <  SRR19973069.1367221/150‑1 (MQ=60)
                                                                                                                                                   CCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGAT    <  SRR19973069.29450/150‑1 (MQ=60)
                                                                                                                                                     TTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGATAT  >  SRR19973069.982610/1‑150 (MQ=60)
                                                                                                                                                      |                                                                                                                                                    
TTCTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAC‑AATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGATAT  >  CP009273/1385859‑1386156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑