Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,386,008 G→T 100% Q35K (CAG→AAG)  ycjY ← S9 homolog non‑peptidase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,386,0080GT98.9% 866.1 / 3.2 279coding (103/921 nt)ycjYS9 homolog non‑peptidase family protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  major base T (105/169);  minor base . (3/0);  total (109/170)
Fisher's exact test for biased strand distribution p-value = 5.83e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 280 total aligned reads displayed.

AACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGG  >  CP009273/1385863‑1386150
                                                                                                                                                  |                                                                                                                                              
aaCTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTAt                                                                                                                                              <  2:726972/148‑1 (MQ=255)
aaCTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTAt                                                                                                                                              <  1:1684629/148‑1 (MQ=255)
 aCTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTtata                                                                                                                                             <  2:1424574/148‑1 (MQ=255)
    gACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTg                                                                                                                                          <  2:2331618/148‑1 (MQ=255)
     aCGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGa                                                                                                                                         >  2:42547/1‑148 (MQ=255)
       gcgGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACg                                                                                                                                       <  2:2117739/148‑1 (MQ=255)
           cTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGtt                                                                                                                                   >  2:2924846/1‑148 (MQ=255)
            tCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGttt                                                                                                                                  <  1:1793300/148‑1 (MQ=255)
            tCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGttt                                                                                                                                  <  1:972512/148‑1 (MQ=255)
             cgccgcCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTtc                                                                                                                                 <  2:1005188/148‑1 (MQ=255)
              gccgccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTAACGCCCCCACCTGGGTGAGTT‑AACACAAATGCCTTATACTGACGGGTTtca                                                                                                                                >  2:2483181/1‑148 (MQ=255)
              gccgccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTtca                                                                                                                                <  1:2871502/148‑1 (MQ=255)
               ccgccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTtcat                                                                                                                               <  2:2665917/148‑1 (MQ=255)
                cgccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAAATGCCTGATACTGACGGGTTtcatc                                                                                                                              >  2:1933013/1‑148 (MQ=255)
                     cTTTCACCTTGATAAGATGCGTCATTAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAAtt                                                                                                                         <  2:357598/148‑1 (MQ=255)
                      tttCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTATTTAACGCCCCCACCGGGGTGCGGT‑AACACAAATGCCATATAATGAAGGGTTTCATCAAAttt                                                                                                                        >  1:1419503/1‑148 (MQ=255)
                       ttCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAAtttt                                                                                                                       >  2:3506050/1‑148 (MQ=255)
                       ttCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAAtttt                                                                                                                       <  1:2566439/148‑1 (MQ=255)
                           ccTTGATTAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTTTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAg                                                                                                                   <  2:1165422/148‑1 (MQ=255)
                              tGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGa                                                                                                                >  2:593675/1‑148 (MQ=255)
                               gATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGaa                                                                                                               <  2:3138656/148‑1 (MQ=255)
                                 tAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTAAAGCCCCCCCCGGGGTGTGAT‑AAAACAAGTGGCTTATAATGACGGGTTTCATCAAATTTTGGGGGGtaaa                                                                                                             >  1:3544764/1‑147 (MQ=255)
                                 tAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAAt                                                                                                             <  1:759339/148‑1 (MQ=255)
                                 tAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAAt                                                                                                             <  1:1566449/148‑1 (MQ=255)
                                  aaGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGaaaaa                                                                                                            >  2:296700/1‑146 (MQ=255)
                                   aGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAg                                                                                                           >  2:2574642/1‑148 (MQ=255)
                                   aGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTATTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTACTCAAATTTTGGTGGGAAATat                                                                                                           >  2:2843886/1‑147 (MQ=255)
                                    gATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAACGCCCCCCCCTGGGTGTGAT‑AACACAATTGCCTTATACTGGCGGGTGTCCTCAAATTTTGGAGGGAAATAGa                                                                                                          >  2:1189453/1‑148 (MQ=255)
                                     aTGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAAACCCCCCCCCCGGGGGGGATAAAAAAAATTGGGT‑ATACTGACGGGGTTGTTCAAAATTTGTTTGGGAATAGAt                                                                                                         >  1:3305865/1‑148 (MQ=255)
                                        cGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCAc                                                                                                      <  2:3529782/148‑1 (MQ=255)
                                          tCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAAAGCCCCCCCCGGGGGGAGAT‑AACACAAATGCCTTATAATGACGGGTTTGATCAAATTTTTGTGGGAAATATATCACTg                                                                                                    >  1:2666332/1‑148 (MQ=255)
                                           cATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTTTTGTTTAACGCCCCCCCCAGGGGGAGAT‑AACACAAATTGCTTATAATTAACGGTTTAATAAAAATTTTGTGGGAAAAATATAACTga                                                                                                   >  1:2661384/1‑147 (MQ=255)
                                             tAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAg                                                                                                 <  1:1322535/148‑1 (MQ=255)
                                                 cAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACaa                                                                                             <  1:3146760/148‑1 (MQ=255)
                                                 cAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACaa                                                                                             <  2:3253829/148‑1 (MQ=255)
                                                  aaTAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACaaa                                                                                            <  1:3027752/148‑1 (MQ=255)
                                                   aTAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTTAAGCCCCCACCAGGGTGAGAT‑AACACAATTGCCTTATAATGACCGGTTTCATAAAATTTTTGAGGGAAATAGATAACTGCAGACAAag                                                                                           >  2:3365827/1‑148 (MQ=255)
                                                     aGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAagag                                                                                         <  2:2462711/148‑1 (MQ=255)
                                                     aGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAagag                                                                                         <  2:1601044/148‑1 (MQ=255)
                                                     aGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGGTGGTTAAAGCCCCCCCCCGGGGGTGAT‑AACACAAATTGCTTATAATTACGGGTTTGAAAAAAATTTTGTGGGGAATAGATAAAAGCAGACAAaaag                                                                                         >  1:482503/1‑148 (MQ=255)
                                                          cAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGt                                                                                    <  1:2624936/148‑1 (MQ=255)
                                                              tCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGa                                                                                <  1:1540491/148‑1 (MQ=255)
                                                               cccTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGat                                                                               <  2:242503/148‑1 (MQ=255)
                                                               cccTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAAAGCCCCCCCCCGGGGGTGAT‑AACACAAATGCCGTATAATGACGGGTTTAATCAAAATTTTGTGGGAAATAGATAAAAGCAGACAAAGAGATTGTTggtg                                                                               >  1:3479444/1‑146 (MQ=255)
                                                                 cTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGatta                                                                             <  2:1344170/148‑1 (MQ=255)
                                                                   tttCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGattatt                                                                           <  2:2035339/148‑1 (MQ=255)
                                                                   tttCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGattatt                                                                           <  1:2955534/148‑1 (MQ=255)
                                                                    ttCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTg                                                                          <  2:682394/148‑1 (MQ=255)
                                                                    ttCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAAAGCCCCCCCCGGGGTGGGATAAAAAAAATTGCGT‑ATACTGACGGGTTTAATCAAATTTTGGTGGGAAATATATAAAAGCAGACAAACAGTTTGTTGGTTTATTg                                                                          >  1:895402/1‑148 (MQ=255)
                                                                       cGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAAATTCCTTATAAAGACGGGTTTAATCAAATTTTGGAGGGAAATATATAACTGCAGACAAAGAGATGGTTGGATTATTTCTg                                                                       >  2:1684789/1‑148 (MQ=255)
                                                                       cGCCAGTTTTTTGGAATAGGTTCCGGCCGTTTGTTATTTAACGCCACCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTg                                                                       <  1:2083740/148‑1 (MQ=255)
                                                                        gCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGatt                                                                              >  2:2056185/1‑140 (MQ=255)
                                                                          cAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTa                                                                    <  2:451273/148‑1 (MQ=255)
                                                                          cAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCACCAGGGTGAGAT‑AACACAAATTCCTTATAATGACGGGTTTCATCAAAATTTTGAGGGAAATAGATAACAGCAGACAAAGAGATGGGTGGATTATTTCTGTTa                                                                    >  2:1574557/1‑148 (MQ=255)
                                                                          cAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAAAGCCCCCACCAGGGTGAGAT‑AACACAAATTCCTTATAATGACGGGTTTCATCAAATTTTTGTGGGAAATAGATCACTGCCGACAAAGAGATGGTTGGGTTATTTCTGTTa                                                                    >  2:1058059/1‑148 (MQ=255)
                                                                           aGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAg                                                                   <  1:1207001/148‑1 (MQ=255)
                                                                           aGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAg                                                                   <  1:1211066/148‑1 (MQ=255)
                                                                            gTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGt                                                                  <  2:1858992/148‑1 (MQ=255)
                                                                             ttttttGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTg                                                                 <  1:955371/148‑1 (MQ=255)
                                                                             ttttttGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTg                                                                 <  1:1341642/148‑1 (MQ=255)
                                                                              tttttGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGa                                                                <  2:2292654/148‑1 (MQ=255)
                                                                               ttttGGCATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCACCTGGGTGAGAT‑AACACAATTGCCGTATAATTACGGGTTTCATCAAATTTTGGAGGGAAATAGATAACTGCAGACAAAGAGATGGTTGGATTATTTCTGTTAGTGaa                                                               >  2:2380803/1‑148 (MQ=255)
                                                                                   ggCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTg                                                           <  1:1021783/148‑1 (MQ=255)
                                                                                    gCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGa                                                          <  1:308349/148‑1 (MQ=255)
                                                                                      aTAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACt                                                        <  2:264386/148‑1 (MQ=255)
                                                                                       tAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACtt                                                       <  2:1969995/148‑1 (MQ=255)
                                                                                        aGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACttt                                                      <  1:1661396/148‑1 (MQ=255)
                                                                                         ggTTCCGGCCGTTTGTTGTTTAACGCCCCCACCTGGGTGAGAT‑AAAAAAAATGCCTTATACTGACGGGGTTCATAAAATTTTGGATGGAAATAGATAAAAGCAGTCAAAGAGATGGTTGGTTTATTGCTGTTAGTGAAGCTGAATtta                                                     >  2:3439410/1‑148 (MQ=255)
                                                                                            tCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTttatta                                                  <  2:959264/148‑1 (MQ=255)
                                                                                                 ccGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtcat                                             <  1:1004726/148‑1 (MQ=255)
                                                                                                  cGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtcatc                                            >  1:2567879/1‑148 (MQ=255)
                                                                                                  cGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtcatc                                            <  1:2454478/148‑1 (MQ=255)
                                                                                                  cGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtcatc                                            <  2:3221121/148‑1 (MQ=255)
                                                                                                  cGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtcatc                                            <  1:2771695/148‑1 (MQ=255)
                                                                                                  cGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtcatc                                            <  2:1447769/148‑1 (MQ=255)
                                                                                                     ttGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCAtt                                         <  2:1097772/148‑1 (MQ=255)
                                                                                                         tCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGt                                     >  2:3371986/1‑148 (MQ=255)
                                                                                                            ttAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTcc                                  <  1:422743/148‑1 (MQ=255)
                                                                                                            ttAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTcc                                  <  2:482503/148‑1 (MQ=255)
                                                                                                            ttAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTcc                                  >  2:86227/1‑148 (MQ=255)
                                                                                                             tAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCt                                 <  2:135386/148‑1 (MQ=255)
                                                                                                             tAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCt                                 >  2:271119/1‑148 (MQ=255)
                                                                                                              aaCGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTc                                >  2:1495648/1‑148 (MQ=255)
                                                                                                                cGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCAt                              >  2:1230006/1‑148 (MQ=255)
                                                                                                                      cACCTGGGTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGtt                        <  2:2223614/148‑1 (MQ=255)
                                                                                                                      cACCTGGGTGAGAT‑AACACAAGTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGtt                        <  2:1686175/148‑1 (MQ=255)
                                                                                                                        gcTGTGTGAGAT‑AACACTATTGCCTTATACTGTGGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGTTTTTTGGTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGt                      <  1:2154936/147‑1 (MQ=255)
                                                                                                                           gggTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCgg                   >  2:2945809/1‑148 (MQ=255)
                                                                                                                             gTGAGAT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGtt                 >  1:1989214/1‑148 (MQ=255)
                                                                                                                                agaT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGt              <  2:775270/148‑1 (MQ=255)
                                                                                                                                agaT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGt              <  1:1819429/148‑1 (MQ=255)
                                                                                                                                  aT‑AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGttt            >  1:2266921/1‑148 (MQ=255)
                                                                                                                                     aaCACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTa          >  2:3122229/1‑148 (MQ=255)
                                                                                                                                     aaCACAATTGCCTTATACTGACGGGTTTAATCACATGTTGGAGGGAAATAGATCACTGAAGACAAAGAGATGGATGGATTATTGCTGTTAGTGAAGATGACTTTATTATTCATCATTACTTTAATCATGGAGTTGTCGGTTCGTTTTa          >  2:2779405/1‑148 (MQ=255)
                                                                                                                                      acacAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTaa         >  1:1656617/1‑148 (MQ=255)
                                                                                                                                        acaATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACg       <  1:2763567/148‑1 (MQ=255)
                                                                                                                                         caATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACgg      <  2:1179915/148‑1 (MQ=255)
                                                                                                                                         caATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACgg      <  2:2681384/148‑1 (MQ=255)
                                                                                                                                           aTTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGtt    >  1:1067661/1‑148 (MQ=255)
                                                                                                                                             tGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTgg  >  2:3262666/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
AACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGG  >  CP009273/1385863‑1386150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGATATC  >  CP009273/1385861‑1386157
                                                                                                                                                     |                                                                                                                                                     
CTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTAT                                                                                                                                                     <  SRR19973066.2955728/150‑1 (MQ=60)
 TAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGTGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATA                                                                                                                                                    <  SRR19973066.2021472/150‑1 (MQ=60)
  AACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATAC                                                                                                                                                   <  SRR19973066.1696898/150‑1 (MQ=60)
      GACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGAC                                                                                                                                               <  SRR19973066.383165/150‑1 (MQ=60)
         GCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGG                                                                                                                                            <  SRR19973066.3349163/150‑1 (MQ=60)
              TCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCA                                                                                                                                       <  SRR19973066.1806060/150‑1 (MQ=60)
              TCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCA                                                                                                                                       <  SRR19973066.1805483/150‑1 (MQ=60)
              TCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCA                                                                                                                                       <  SRR19973066.979663/150‑1 (MQ=60)
                GCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATC                                                                                                                                     <  SRR19973066.2890761/150‑1 (MQ=60)
                  CGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AAATTCCTTATACTTACGGGTTTAATCAA                                                                                                                                   >  SRR19973066.2683485/1‑150 (MQ=60)
                      ACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTATTTAACGCCCCCACCGGGGTGCGGT‑AACAC‑AAATGCCATATAATGAAGGGTTTCATCAAATTT                                                                                                                               >  SRR19973066.1429666/1‑150 (MQ=60)
                         TTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGG                                                                                                                            <  SRR19973066.2583855/150‑1 (MQ=60)
                         TTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGG                                                                                                                            <  SRR19973066.783930/150‑1 (MQ=60)
                          TCACCTTGATAAGATGTGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAAAGCCCCCCCCAGGGGGAGAT‑AACAC‑AATTGCCGTATAATGACGGGTTTAATCAAATTTTGGA                                                                                                                           >  SRR19973066.2613421/1‑150 (MQ=60)
                          TCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTAAAGCCCCCCCCTGGGTGAGAT‑AAAAC‑AATTGCCGTATACTGACGGGGTTAATCAAATTTTGGA                                                                                                                           >  SRR19973066.2324420/1‑150 (MQ=60)
                           CACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAG                                                                                                                          <  SRR19973066.2762286/150‑1 (MQ=60)
                            ACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGG                                                                                                                         <  SRR19973066.1641703/150‑1 (MQ=60)
                                 GATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTAAAGCCCCCCCCGGGGTGTGAT‑AAAAC‑AAGTGGCTTATAATGACGGGTTTCATCAAATTTTGGggggtaaa                                                                                                                    >  SRR19973066.3568304/1‑142 (MQ=60)
                                   TAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAG                                                                                                                  <  SRR19973066.1577733/150‑1 (MQ=60)
                                   TAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAG                                                                                                                  <  SRR19973066.764962/150‑1 (MQ=60)
                                     AGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGAT                                                                                                                <  SRR19973066.2592109/150‑1 (MQ=60)
                                     AGATGCGTCATAAGCAATAGTGACAAATCCATTTTCCGCCAGTTTTTTGGCTTAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGAT                                                                                                                <  SRR19973066.1689499/150‑1 (MQ=60)
                                         GCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACT                                                                                                            <  SRR19973066.901504/150‑1 (MQ=60)
                                         GCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACT                                                                                                            <  SRR19973066.3156086/150‑1 (MQ=60)
                                          CGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTG                                                                                                           <  SRR19973066.2995119/150‑1 (MQ=60)
                                          CGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAAAGCCCCCCCCGGGGGGAGAT‑AACAC‑AAATGCCTTATAATGACGGGTTTGATCAAATTTTTGTGGGAAATATATCACTG                                                                                                           >  SRR19973066.2684176/1‑150 (MQ=60)
                                           GTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAACGCCCCCCCCAGGGTGTGAT‑AACAC‑AAATTGCTTATAATGACGGGTTTCATGAAATTTTTGAGGGCAATAGATAACTGC                                                                                                          >  SRR19973066.2652997/1‑150 (MQ=60)
                                              ATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAAAGCCCCCCCCCGGGGGAGAT‑AACAC‑AAATGCCGTATACAGTCGGGGTTCATCAAAATTTGGTGGGCAATAGATCACAGCAGA                                                                                                       >  SRR19973066.3190259/1‑150 (MQ=60)
                                               TAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGAC                                                                                                      <  SRR19973066.105629/150‑1 (MQ=60)
                                               TAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGAC                                                                                                      <  SRR19973066.1332119/150‑1 (MQ=60)
                                                   CAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAG                                                                                                  <  SRR19973066.2327771/150‑1 (MQ=60)
                                                   CAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAG                                                                                                  <  SRR19973066.3167745/150‑1 (MQ=60)
                                                    AATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGA                                                                                                 <  SRR19973066.3048851/150‑1 (MQ=60)
                                                    AATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGA                                                                                                 <  SRR19973066.3047975/150‑1 (MQ=60)
                                                    AATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGA                                                                                                 <  SRR19973066.3047981/150‑1 (MQ=60)
                                                            CAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTG                                                                                         <  SRR19973066.2642788/150‑1 (MQ=60)
                                                            CAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTG                                                                                         <  SRR19973066.2642635/150‑1 (MQ=60)
                                                               ATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAAAGCCCCCCCCCGGGGGTGAT‑AACAC‑AAATGCCGTATAATGACGGGTTTAATCAAAATTTTGTGGGAAATAGATAAAAGCAGACAAAGAGATTGTTGGtg                                                                                      >  SRR19973066.3502665/1‑148 (MQ=50)
                                                                TCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATT                                                                                     <  SRR19973066.1552012/150‑1 (MQ=60)
                                                                TCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATT                                                                                     <  SRR19973066.1551497/150‑1 (MQ=60)
                                                                  cgatcTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTAT                                                                                   <  SRR19973066.2070454/145‑1 (MQ=60)
                                                                    TTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAAAGCCCCCCCCGGGGTGGGATAAAAAA‑AATTGCGT‑ATACTGACGGGTTTAATCAAATTTTGGTGGGAAATAtataaaagcagacaaacagtttgttggtttattg                                                                                 >  SRR19973066.901735/1‑116 (MQ=48)
                                                                     TTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGC                                                                                <  SRR19973066.975364/150‑1 (MQ=60)
                                                                     TTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGC                                                                                <  SRR19973066.2975125/150‑1 (MQ=60)
                                                                         CGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTT                                                                            <  SRR19973066.286027/150‑1 (MQ=60)
                                                                         CGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAAAGCCCCCACCGGGGGGAGAT‑AACACAAATTGCGT‑ATAATGACGGGTTTTATCAAATTTTTGGGGGGAATAGATCACTGCACACAAAGAGATGGgtggggtttttgtgtt                                                                            >  SRR19973066.2938219/1‑134 (MQ=56)
                                                                         CGCCAGTTTTTTGGAATAGGTTCCGGCCGTTTGTTATTTAACGCCACCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTT                                                                            <  SRR19973066.2098115/150‑1 (MQ=60)
                                                                           CCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTAACGCCCCCACCCGGGGGTGTT‑AACACAAATTGCGT‑ATAAAGACGGGGTTCATAAAAATTTTGAGGGAAATAAATAACTGCAGAAAAAGAGATGGTTGGTTTATTGCTTTTAG                                                                          >  SRR19973066.2401486/1‑150 (MQ=38)
                                                                             AGTTTTTTGTCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTG                                                                        <  SRR19973066.1219539/150‑1 (MQ=60)
                                                                             AGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTG                                                                        <  SRR19973066.1219540/150‑1 (MQ=60)
                                                                             AGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTG                                                                        <  SRR19973066.1215422/150‑1 (MQ=60)
                                                                             AGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTG                                                                        <  SRR19973066.3004748/150‑1 (MQ=60)
                                                                             AGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTG                                                                        <  SRR19973066.1219547/150‑1 (MQ=60)
                                                                               TTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAA                                                                      <  SRR19973066.962360/150‑1 (MQ=60)
                                                                               TTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAA                                                                      <  SRR19973066.1351381/150‑1 (MQ=60)
                                                                               TTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAA                                                                      <  SRR19973066.1351451/150‑1 (MQ=60)
                                                                               TTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AAGTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAA                                                                      <  SRR19973066.1349499/150‑1 (MQ=60)
                                                                                TTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAG                                                                     <  SRR19973066.1156418/150‑1 (MQ=60)
                                                                                  TTTGGCATAGGTTCCGGCCGTTTGTTATTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCT                                                                   <  SRR19973066.1179773/150‑1 (MQ=60)
                                                                                  TTTGGCATAGGTTCCGGCCGTGTGTTATTTAAAGCCCCCACCAGGGTGAGAT‑AAAAA‑AAATGCCGTATAATGACGGGTTTCATGAAAATTTTGTGGGAAATAGATaacatcacaccaagagatgggtggcttgttggtgttagtgaatca                                                                   >  SRR19973066.2979132/1‑105 (MQ=23)
                                                                                     GGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGAC                                                                <  SRR19973066.1029291/150‑1 (MQ=60)
                                                                                     GGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGAC                                                                <  SRR19973066.1029300/150‑1 (MQ=60)
                                                                                      GCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACT                                                               <  SRR19973066.310596/150‑1 (MQ=60)
                                                                                      GCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACT                                                               <  SRR19973066.2120793/150‑1 (MQ=60)
                                                                                          AGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTAT                                                           <  SRR19973066.1671197/150‑1 (MQ=60)
                                                                                          AGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTAT                                                           <  SRR19973066.1673501/150‑1 (MQ=60)
                                                                                              TCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATT                                                       <  SRR19973066.597787/150‑1 (MQ=60)
                                                                                                  GCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATC                                                   >  SRR19973066.2585291/1‑150 (MQ=60)
                                                                                                  GCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATC                                                   <  SRR19973066.1846526/150‑1 (MQ=60)
                                                                                                  GCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATC                                                   >  SRR19973066.2585305/1‑150 (MQ=60)
                                                                                                  GCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATC                                                   <  SRR19973066.25761/150‑1 (MQ=60)
                                                                                                   CCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCA                                                  <  SRR19973066.1012128/150‑1 (MQ=60)
                                                                                                    CGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCAT                                                 <  SRR19973066.2790361/150‑1 (MQ=60)
                                                                                                    CGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCAT                                                 <  SRR19973066.1697061/150‑1 (MQ=60)
                                                                                                    CGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCAT                                                 <  SRR19973066.2471201/150‑1 (MQ=60)
                                                                                                    CGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCAT                                                 <  SRR19973066.2790310/150‑1 (MQ=60)
                                                                                                      TTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTC                                               >  SRR19973066.1985376/1‑150 (MQ=60)
                                                                                                       TTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGTGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCC                                              <  SRR19973066.38085/150‑1 (MQ=60)
                                                                                                        TGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGNAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCG                                             >  SRR19973066.1819769/1‑150 (MQ=60)
                                                                                                        TGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCG                                             <  SRR19973066.2933008/150‑1 (MQ=60)
                                                                                                        TGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCG                                             <  SRR19973066.2933001/150‑1 (MQ=60)
                                                                                                        TGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCG                                             >  SRR19973066.2542011/1‑150 (MQ=60)
                                                                                                          TTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTT                                           <  SRR19973066.3529417/150‑1 (MQ=60)
                                                                                                              TTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTC                                       <  SRR19973066.426025/150‑1 (MQ=60)
                                                                                                                AACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCAT                                     <  SRR19973066.2282044/150‑1 (MQ=60)
                                                                                                                 ACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATG                                    <  SRR19973066.1397355/150‑1 (MQ=60)
                                                                                                                   GCCCCCACCAGGGTGAGAT‑AACAC‑AAATTCCTTATAATTACGGGTTTCAAAAAATTTTTGAGGGAAATATATCACAGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTAATAATTACGTTCCTCATGGA                                  >  SRR19973066.1674480/1‑150 (MQ=60)
                                                                                                                    CCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAG                                 >  SRR19973066.1558619/1‑150 (MQ=60)
                                                                                                                      CCCACCTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTT                               <  SRR19973066.3315488/150‑1 (MQ=60)
                                                                                                                          gctgtGTGAGAT‑AACAC‑TATTGCCTTATACTGTGGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGTTTTTTGGTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCG                           <  SRR19973066.2169900/145‑1 (MQ=60)
                                                                                                                           CTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGG                          >  SRR19973066.916971/1‑150 (MQ=60)
                                                                                                                           CTGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGG                          >  SRR19973066.916900/1‑150 (MQ=60)
                                                                                                                            TGGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGT                         >  SRR19973066.12754/1‑150 (MQ=60)
                                                                                                                             GGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTT                        <  SRR19973066.3173855/150‑1 (MQ=60)
                                                                                                                             GGGTGAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTT                        >  SRR19973066.2003226/1‑150 (MQ=60)
                                                                                                                                 GAGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTT                    >  SRR19973066.2890665/1‑150 (MQ=60)
                                                                                                                                  AGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTT                   >  SRR19973066.2282513/1‑150 (MQ=60)
                                                                                                                                  AGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTT                   <  SRR19973066.1832395/150‑1 (MQ=60)
                                                                                                                                  AGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTT                   <  SRR19973066.1431598/150‑1 (MQ=60)
                                                                                                                                  AGAT‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCAGTTCCTCATGGAGATGTCGGTTCGTTT                   >  SRR19973066.2167667/1‑150 (MQ=60)
                                                                                                                                  AGAT‑AACAC‑AAGTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACATAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTT                   <  SRR19973066.1431574/150‑1 (MQ=60)
                                                                                                                                     T‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAA                >  SRR19973066.1668682/1‑150 (MQ=60)
                                                                                                                                     T‑AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAA                >  SRR19973066.1668685/1‑150 (MQ=60)
                                                                                                                                       AACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTNGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAAC               <  SRR19973066.1818835/150‑1 (MQ=60)
                                                                                                                                        ACAC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACG              <  SRR19973066.3450315/150‑1 (MQ=60)
                                                                                                                                          AC‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGT            <  SRR19973066.2782176/150‑1 (MQ=60)
                                                                                                                                           C‑AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTT           >  SRR19973066.1075489/1‑150 (MQ=60)
                                                                                                                                                 GCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGA      >  SRR19973066.3265536/1‑150 (MQ=60)
                                                                                                                                                   CTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGATA    <  SRR19973066.2379426/150‑1 (MQ=60)
                                                                                                                                                     TATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGGTGTCGGTTCGTTTTAACGGTTGGTGATATC  >  SRR19973066.2580313/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGAT‑AACAC‑AATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGATATC  >  CP009273/1385861‑1386157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑