Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,386,008 G→T 100% Q35K (CAG→AAG)  ycjY ← S9 homolog non‑peptidase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,386,0080GT99.2% 869.6 / ‑3.4 273Q35K (CAG→AAG) ycjYS9 homolog non‑peptidase family protein
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base T (122/143);  total (125/148)
Fisher's exact test for biased strand distribution p-value = 3.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 278 total aligned reads displayed.

CTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGAT  >  CP009273/1385861‑1386154
                                                                                                                                                   |                                                                                                                                                  
cTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTAt                                                                                                                                                  <  1:2174079/150‑1 (MQ=255)
 tAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGTTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTtata                                                                                                                                                 >  1:4186502/1‑150 (MQ=255)
 tAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTtata                                                                                                                                                 <  2:4323993/150‑1 (MQ=255)
  aaCTGACGCGGCTCTCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATAc                                                                                                                                                <  1:2667270/150‑1 (MQ=255)
  aaCTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATAc                                                                                                                                                >  1:2710472/1‑150 (MQ=255)
  aaCTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATAc                                                                                                                                                >  1:5088458/1‑150 (MQ=255)
  aaCTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATAc                                                                                                                                                >  2:1596322/1‑150 (MQ=255)
      gACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGAc                                                                                                                                            <  1:387077/150‑1 (MQ=255)
      gACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGAc                                                                                                                                            >  2:1482239/1‑150 (MQ=255)
         gcgGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACggg                                                                                                                                         <  1:4711273/150‑1 (MQ=255)
          cgGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACAGACGGGt                                                                                                                                        >  1:2706726/1‑150 (MQ=255)
              tCGCCGCCACTTTCACCTGTATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTtca                                                                                                                                    <  2:5093142/150‑1 (MQ=255)
               cgccgcCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTTTTATTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTtcat                                                                                                                                   >  2:4245055/1‑150 (MQ=255)
               cgccgcCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTtcat                                                                                                                                   <  2:2919582/150‑1 (MQ=255)
                gccgccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTtcatc                                                                                                                                  <  1:3760057/150‑1 (MQ=255)
                gccgccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTtcatc                                                                                                                                  <  1:3002436/150‑1 (MQ=255)
                gccgccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTtcatc                                                                                                                                  <  2:3683051/150‑1 (MQ=255)
                gccgccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGATTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTtcatc                                                                                                                                  <  2:1220422/150‑1 (MQ=255)
                 ccgccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTtcatca                                                                                                                                 <  1:1293201/150‑1 (MQ=255)
                  cgccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTATCGCCCCCAACGGGGTGAGATAACCCAATTGCCTGATACTGACGGTTGtcattat                                                                                                                                >  2:2615244/1‑147 (MQ=255)
                    ccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAAt                                                                                                                              >  2:3704610/1‑150 (MQ=255)
                    ccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCCCCAGGGGGGGATAAAACAAATTCCGTATACAGACGGGTTTCATCaaaa                                                                                                                              >  1:901609/1‑149 (MQ=255)
                     cACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAAtt                                                                                                                             <  2:606995/150‑1 (MQ=255)
                       cTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAAtttt                                                                                                                           >  1:2871982/1‑150 (MQ=255)
                       cTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAAtttt                                                                                                                           <  2:1718675/150‑1 (MQ=255)
                       cTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAAtttt                                                                                                                           <  1:1586260/150‑1 (MQ=255)
                          tCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGa                                                                                                                        <  1:1077207/150‑1 (MQ=255)
                          tCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAACGCCCCCCCCAGGGTGAGATAACACAAATGCCTTATACTGACGGGGTTCATCAAATTTTGGa                                                                                                                        >  1:4466718/1‑150 (MQ=255)
                                 gATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAAt                                                                                                                 <  2:2230941/150‑1 (MQ=255)
                                   tAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAg                                                                                                               <  1:3421826/150‑1 (MQ=255)
                                    aaGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTCATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGa                                                                                                              <  2:3462964/150‑1 (MQ=255)
                                            tCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCa                                                                                                      <  1:3216621/150‑1 (MQ=255)
                                             cATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAg                                                                                                     <  1:4120963/150‑1 (MQ=255)
                                             cATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAg                                                                                                     <  1:3313233/150‑1 (MQ=255)
                                              aTAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTTGAGGGAAATATATCACTGCAGa                                                                                                    >  1:2858871/1‑150 (MQ=255)
                                                aaGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGGTCCGGCCGTTTGTTCTTTAAAGCCCCCCCCTGGGTGCGATAACAAAATTGCCGTATACAGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACa                                                                                                  >  2:4771291/1‑150 (MQ=255)
                                                 aGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCGGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACaa                                                                                                 >  1:348669/1‑150 (MQ=255)
                                                   cAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACACag                                                                                               >  2:118656/1‑150 (MQ=255)
                                                   cAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAag                                                                                               <  2:303400/150‑1 (MQ=255)
                                                    aaTAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGTGGGAAATAGATCACTGCAGACAAaga                                                                                              >  2:2478321/1‑150 (MQ=255)
                                                      tAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAgaga                                                                                            <  2:250908/150‑1 (MQ=255)
                                                       aGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGAt                                                                                           >  2:3190422/1‑150 (MQ=255)
                                                       aGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATAATGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGAt                                                                                           >  2:3825315/1‑150 (MQ=255)
                                                       aGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTTTTCTTTAACGCCCCCACCGGGGGGAGATAACACAATTTCCTTATACTGACGGGTTTAATCAAATTTTTGATGGAAATATATCACAGCAGAAAAAGAGAt                                                                                           >  1:1006523/1‑150 (MQ=255)
                                                        gTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACAGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATg                                                                                          >  1:4987754/1‑150 (MQ=255)
                                                          gACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGt                                                                                        <  1:4335103/150‑1 (MQ=255)
                                                          gACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGt                                                                                        <  1:1946684/150‑1 (MQ=255)
                                                               aTCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGat                                                                                   <  2:4916017/150‑1 (MQ=255)
                                                               aTCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAAAAATTCCGTATACAGACGGGGTTCAACAAATTTTGGAGGGAAATATATAACAGCAGACAAAGAGATGGTTGGat                                                                                   >  1:99674/1‑150 (MQ=255)
                                                               aTCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCGGGGTGAGATAACACAAATGCCTTATACTGACGGGGTTCATCAAAATTTGGTGGGGAATATATAACTGCAGACAAAGAGATGGGTGGat                                                                                   >  1:4909185/1‑150 (MQ=255)
                                                                tCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCCCAGGGGTTAGATAAAACAATTGCCTTATACTGACGGGTTTCATAAAATTTAGGAGGGGAATAGATCACTGCACACACAGTGTTGGTTGGatt                                                                                  >  2:2669793/1‑150 (MQ=255)
                                                                tCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACTCAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGatt                                                                                  <  1:1781772/150‑1 (MQ=255)
                                                                tCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGatt                                                                                  <  2:1055822/150‑1 (MQ=255)
                                                                    ttttCCGGCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTTAAGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTGTCATCAACGTTTGGGGGGAAATAGATAAAGTCAGACCAGGTGATGGTTGGGttgttg                                                                              >  2:1777386/1‑150 (MQ=255)
                                                                      ttCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCt                                                                            >  2:2566647/1‑150 (MQ=255)
                                                                       tCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAAATGCCTTATAATGACGGGTTTCAACAAAATTTGGAGGGAAAAAGATCACTGCAGACAAAGAGAGGGGTGGATTATTGCTg                                                                           >  1:5009579/1‑150 (MQ=255)
                                                                       tCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCGGGGTGAGTTAACACAATTGCCTTATACTGACGGGTTTAATCAAATTTTGGAGGGCAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTg                                                                           >  1:687287/1‑150 (MQ=255)
                                                                          gCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAAAACAATTTCCTTATACTGACGGGTTTCATCAAATTTTGGTGGGAAATAGATCACTGCAGACAAAGAGCTGGTTGGATTATTGGTGTTa                                                                        >  1:3635959/1‑150 (MQ=255)
                                                                          gCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCACCAGGGTGTGATAACACAATTGCCTTATAATGACGGGTTTCATCAAATTTTGGAGGGAAATATATAACTGCAGACAAAGAGATGGTTGGATTATTTCTGTTa                                                                        >  1:4621420/1‑150 (MQ=255)
                                                                            cAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGt                                                                      <  2:4568616/150‑1 (MQ=255)
                                                                            cAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGt                                                                      <  1:1435854/150‑1 (MQ=255)
                                                                             aGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTg                                                                     >  2:828187/1‑150 (MQ=255)
                                                                             aGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTg                                                                     <  1:4669386/150‑1 (MQ=255)
                                                                              gTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGa                                                                    <  2:5119843/150‑1 (MQ=255)
                                                                                    tGGCATAGGTTCCGGCCGTGTGTTCTTTAACGCCCCCACCTGGGTTAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAAATTTGTAGGGAAATAGATCACTGCCGACCAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGa                                                              >  2:2160093/1‑150 (MQ=255)
                                                                                     ggCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGAc                                                             <  2:3932545/150‑1 (MQ=255)
                                                                                         tAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTtta                                                         <  2:4129174/150‑1 (MQ=255)
                                                                                         tAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTtta                                                         <  2:1169928/150‑1 (MQ=255)
                                                                                         tAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTAATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTtta                                                         <  2:2830804/150‑1 (MQ=255)
                                                                                            gTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTttatta                                                      <  1:4134573/150‑1 (MQ=255)
                                                                                                cGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtca                                                  <  2:2088273/150‑1 (MQ=255)
                                                                                                 ggCCGTTTGTTCTTTAACCCCCCCACCAGGGTGAGATAACACAATTTCCTTATACTGACGGGTTTAATAAAAATTTGGATGGAAATATATAACTGCAGACAAAGAGATGGTTGGATTATTTCTGTTAGTGAAGCTGACTTTATTATtaat                                                 >  1:3959102/1‑150 (MQ=255)
                                                                                                       ttGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTcc                                           >  1:4162733/1‑150 (MQ=255)
                                                                                                          ttCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGtt                                        >  1:894347/1‑150 (MQ=255)
                                                                                                           tCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTc                                       <  2:2871982/150‑1 (MQ=255)
                                                                                                             tttAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCt                                     <  1:4316176/150‑1 (MQ=255)
                                                                                                              ttAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTc                                    >  1:4547792/1‑150 (MQ=255)
                                                                                                               tAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCa                                   >  2:4425379/1‑150 (MQ=255)
                                                                                                                aaCGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCAt                                  >  1:2377690/1‑150 (MQ=255)
                                                                                                                  cGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATgg                                <  2:1903363/150‑1 (MQ=255)
                                                                                                                   gCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGa                               >  2:1464644/1‑150 (MQ=255)
                                                                                                                    cccccACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAg                              >  1:2594295/1‑150 (MQ=255)
                                                                                                                       ccACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTg                           <  2:3976647/150‑1 (MQ=255)
                                                                                                                        cACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGt                          <  2:1969648/150‑1 (MQ=255)
                                                                                                                        cACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGt                          <  2:2315737/150‑1 (MQ=255)
                                                                                                                         aCCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTc                         <  1:3704610/150‑1 (MQ=255)
                                                                                                                          ccTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCg                        >  1:4871065/1‑150 (MQ=255)
                                                                                                                             gggTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGtt                     >  1:124614/1‑150 (MQ=255)
                                                                                                                              tgTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTc                    >  2:5133849/2‑150 (MQ=255)
                                                                                                                              ggTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTc                    <  2:714364/150‑1 (MQ=255)
                                                                                                                                tGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGt                  >  2:407565/1‑150 (MQ=255)
                                                                                                                                tGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTGGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGt                  <  2:3635959/150‑1 (MQ=255)
                                                                                                                                      aaCACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAAc            >  2:2229139/1‑150 (MQ=255)
                                                                                                                                          caATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGtt        <  2:2706836/150‑1 (MQ=255)
                                                                                                                                           aaTTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTg       >  2:3766238/1‑150 (MQ=255)
                                                                                                                                           aaTTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTg       <  2:1412064/150‑1 (MQ=255)
                                                                                                                                            aTTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTgg      >  2:4987332/1‑150 (MQ=255)
                                                                                                                                             ttGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGt     >  1:5142143/1‑150 (MQ=255)
                                                                                                                                             ttGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGt     >  1:185760/1‑150 (MQ=255)
                                                                                                                                              tGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTg    >  1:5113361/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGAT  >  CP009273/1385861‑1386154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGATATC  >  CP009273/1385861‑1386157
                                                                                                                                                   |                                                                                                                                                     
CTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTAT                                                                                                                                                     <  SRR19973063.2180881/150‑1 (MQ=60)
CTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCCCCAGGGTGAGATAACACAAATGCCTTAT                                                                                                                                                     >  SRR19973063.4810435/1‑150 (MQ=60)
 TAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGTTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAAATGCCTTATA                                                                                                                                                    >  SRR19973063.4199867/1‑150 (MQ=60)
 TAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATA                                                                                                                                                    >  SRR19973063.3442155/1‑150 (MQ=60)
  AACTGACGCGGCTCTCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATAC                                                                                                                                                   <  SRR19973063.2675835/150‑1 (MQ=60)
  AACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATAC                                                                                                                                                   >  SRR19973063.2719228/1‑150 (MQ=60)
  AACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATAC                                                                                                                                                   >  SRR19973063.5104836/1‑150 (MQ=60)
  AACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATAC                                                                                                                                                   <  SRR19973063.491051/150‑1 (MQ=60)
  AACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATAC                                                                                                                                                   <  SRR19973063.491055/150‑1 (MQ=60)
     TGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGTGATAACACAATTGCCTTATACTGA                                                                                                                                                >  SRR19973063.4702833/1‑150 (MQ=60)
     TGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGA                                                                                                                                                <  SRR19973063.4957220/150‑1 (MQ=60)
      GACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGAC                                                                                                                                               <  SRR19973063.388178/150‑1 (MQ=60)
      GACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGAC                                                                                                                                               <  SRR19973063.388379/150‑1 (MQ=60)
      GACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGAC                                                                                                                                               <  SRR19973063.388433/150‑1 (MQ=60)
       ACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACG                                                                                                                                              <  SRR19973063.34464/150‑1 (MQ=60)
       ACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACG                                                                                                                                              <  SRR19973063.1794335/150‑1 (MQ=60)
        CGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTTACGCCCCCACCAGGGGGTGATAACACAAATGCCTTATACTGACGG                                                                                                                                             >  SRR19973063.3576841/1‑150 (MQ=60)
         GCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGG                                                                                                                                            <  SRR19973063.4726284/150‑1 (MQ=60)
          CGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACAGACGGGT                                                                                                                                           >  SRR19973063.2715464/1‑150 (MQ=60)
          CGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCACCTGGGTGAGATAACACAATTGCCTTATAATGACGGGT                                                                                                                                           >  SRR19973063.2715575/1‑150 (MQ=60)
               CGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCAT                                                                                                                                      <  SRR19973063.3718602/150‑1 (MQ=60)
                GCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATC                                                                                                                                     <  SRR19973063.3772062/150‑1 (MQ=60)
                GCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATC                                                                                                                                     <  SRR19973063.630164/150‑1 (MQ=60)
                GCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATC                                                                                                                                     <  SRR19973063.3012012/150‑1 (MQ=60)
                GCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATC                                                                                                                                     <  SRR19973063.408658/150‑1 (MQ=60)
                 CCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCA                                                                                                                                    <  SRR19973063.2851430/150‑1 (MQ=60)
                 CCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCA                                                                                                                                    <  SRR19973063.1297050/150‑1 (MQ=60)
                     CACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATT                                                                                                                                <  SRR19973063.3099993/150‑1 (MQ=60)
                      ACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCAGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTT                                                                                                                               <  SRR19973063.3853028/150‑1 (MQ=60)
                       CTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTTCCTTATACTGACGGGTTTCAACAAAATTT                                                                                                                              >  SRR19973063.2881173/1‑150 (MQ=60)
                       CTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTT                                                                                                                              <  SRR19973063.1591118/150‑1 (MQ=60)
                       CTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCACCTGGGTGAGATAACACAAATTCCTTATACTTACGGGTTTCATCAAATTTT                                                                                                                              >  SRR19973063.2881068/1‑150 (MQ=60)
                          TCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGA                                                                                                                           <  SRR19973063.1080480/150‑1 (MQ=60)
                          TCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAACGCCCCCCCCAGGGTGAGATAACACAAATGCCTTATACTGACGGGGTTCATCAAATTTTGGA                                                                                                                           >  SRR19973063.4481050/1‑150 (MQ=60)
                            ACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGG                                                                                                                         >  SRR19973063.1259286/1‑150 (MQ=60)
                              CTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGA                                                                                                                       <  SRR19973063.4576108/150‑1 (MQ=60)
                                TGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTAATCAAATTTTGGAGGGAAA                                                                                                                     >  SRR19973063.1816013/1‑150 (MQ=60)
                                  ATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCGGGGTGAGATAACACAATTGCCTTATACAGACGGGTTTCATCAAATTTTGGAGGGAAATA                                                                                                                   >  SRR19973063.4236377/1‑150 (MQ=60)
                                   TAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAG                                                                                                                  <  SRR19973063.3858447/150‑1 (MQ=60)
                                   TAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAG                                                                                                                  <  SRR19973063.3432712/150‑1 (MQ=60)
                                    AAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGA                                                                                                                 <  SRR19973063.2676435/150‑1 (MQ=60)
                                      GATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCCCCAGGGTGTGATAAAACAATTTCCTTATAATTACGGGTTTCATaaaaattttgtggggaatatata                                                                                                               >  SRR19973063.977813/1‑127 (MQ=60)
                                       ATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCAGGGTGAGATACCACAATTGCCTTAAACAGACGGGTTTCAACAAAATTTTGATGGAAATAGATCA                                                                                                              >  SRR19973063.1304761/1‑150 (MQ=60)
                                        TGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAAAGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGTGGGAAATAGATCAC                                                                                                             >  SRR19973063.4142137/1‑150 (MQ=60)
                                            TCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCA                                                                                                         <  SRR19973063.5104794/150‑1 (MQ=60)
                                            TCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCA                                                                                                         <  SRR19973063.3226961/150‑1 (MQ=60)
                                             CATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAG                                                                                                        <  SRR19973063.4134154/150‑1 (MQ=60)
                                             CATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAG                                                                                                        <  SRR19973063.3323824/150‑1 (MQ=60)
                                              ATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTTGAGGGTAATAGATAACTGCCGA                                                                                                       >  SRR19973063.2868265/1‑150 (MQ=60)
                                              ATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTTGAGGGAAATATATCACTGCAGA                                                                                                       >  SRR19973063.2868030/1‑150 (MQ=60)
                                                 AGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCGGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAA                                                                                                    >  SRR19973063.349674/1‑150 (MQ=60)
                                                     ATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAG                                                                                                <  SRR19973063.174478/150‑1 (MQ=60)
                                                     ATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAG                                                                                                >  SRR19973063.2951442/1‑150 (MQ=60)
                                                     ATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAG                                                                                                <  SRR19973063.3151835/150‑1 (MQ=60)
                                                       AGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACAGACGGGTTTCATCAAAATTTGGAGGGAAATAGATCACTGCAGACAAAGAGAT                                                                                              >  SRR19973063.1198479/1‑150 (MQ=60)
                                                       AGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTTTTCTTTAACGCCCCCACCGGGGGGAGATAACACAATTTCCTTATACTGACGGGTTTAATCAAATTTTTGATGGAAATATATCACAGCAGAAAAAGAGAT                                                                                              >  SRR19973063.1009554/1‑150 (MQ=60)
                                                        GTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATG                                                                                             <  SRR19973063.5143442/150‑1 (MQ=60)
                                                        GTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACAGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATG                                                                                             >  SRR19973063.5003868/1‑150 (MQ=60)
                                                        GTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCACCTGGGTGAGATAACACAATTGCCTTATAATGACGGGTTTCATCAAATTTTGGAGGGAAATAGATAAAAGCAGACAAAGAGATG                                                                                             >  SRR19973063.5003908/1‑150 (MQ=60)
                                                         TGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGGTCTTTTAAGCCCCCACCAGGGTGTGATAACACAAATGCCCTATACAGACGGGTTTCATCAAAATTTTGTGGGGAAAAGATAACTGCAGAACAAGAGATGG                                                                                            >  SRR19973063.898342/1‑150 (MQ=60)
                                                         TGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTTTTGTTTAACCCCCCCCCCAGGGGGTGATAACAAAAATTCCTGATACAGACGGGTTTAATAAAAATTTTGGGGGAAATATAACACAGCAGACAAAGAGAGGG                                                                                            >  SRR19973063.898334/1‑150 (MQ=33)
                                                          GACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGT                                                                                           <  SRR19973063.1776941/150‑1 (MQ=60)
                                                          GACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGT                                                                                           <  SRR19973063.4348917/150‑1 (MQ=60)
                                                          GACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGT                                                                                           <  SRR19973063.1952776/150‑1 (MQ=60)
                                                          GACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGT                                                                                           <  SRR19973063.1952838/150‑1 (MQ=60)
                                                               ATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGAT                                                                                      <  SRR19973063.1042310/150‑1 (MQ=60)
                                                               ATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACAAAAATTCCGTATACAGACGGGGTTCAACAAATTTTGGAGGGAAATATATAACAGCAGACAAAGAGATGGTTGGAT                                                                                      >  SRR19973063.100017/1‑150 (MQ=60)
                                                               ATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCGGGGTGAGATAACACAAATGCCTTATACTGACGGGGTTCATCAAAATTTGGTGGGGAATATATAACTGCAGACAAAGAGATGGGTGGAT                                                                                      >  SRR19973063.4925036/1‑150 (MQ=60)
                                                                TCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACTCAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATT                                                                                     <  SRR19973063.1787274/150‑1 (MQ=60)
                                                                TCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCAGGGGGTGATAACACAATTTCCTTATACTGACGGGTTTCATAAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATT                                                                                     >  SRR19973063.4462982/1‑150 (MQ=60)
                                                                      TTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCT                                                                               <  SRR19973063.934928/150‑1 (MQ=60)
                                                                       TCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAAATGCCTTATAATGACGGGTTTCAACAAAATTTGGAGGGAAAAAGATCACTGCAGACAAAGAGAGGGGTGGATTATTGCTG                                                                              >  SRR19973063.5025750/1‑150 (MQ=60)
                                                                       TCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCGGGGTGAGTTAACACAATTGCCTTATACTGACGGGTTTAATCAAATTTTGGAGGGCAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTG                                                                              >  SRR19973063.689301/1‑150 (MQ=60)
                                                                       TCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTATTTAAAGCCCCCACCGGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGCAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTG                                                                              >  SRR19973063.3340290/1‑150 (MQ=60)
                                                                        CCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCCCCTGGGTGAGATAACACAAATGGCTTATACTGACGGGTTTCATCAAATTTTGGGGGGTAATAGATCACTGCAGACAAAGAGATGGTTGGGTTATTGCTGG                                                                             >  SRR19973063.4782757/1‑150 (MQ=60)
                                                                        CCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGT                                                                             <  SRR19973063.2279332/150‑1 (MQ=60)
                                                                         CGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTT                                                                            <  SRR19973063.615658/150‑1 (MQ=60)
                                                                          GCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAAAACAATTTCCTTATACTGACGGGTTTCATCAAATTTTGGTGGGAAATAGATCACTGCAGACAAAGAGCTGGTTGGATTATTGGTGTTA                                                                           >  SRR19973063.3647625/1‑150 (MQ=60)
                                                                          GCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCACCAGGGTGTGATAACACAATTGCCTTATAATGACGGGTTTCATCAAATTTTGGAGGGAAATATATAACTGCAGACAAAGAGATGGTTGGATTATTTCTGTTA                                                                           >  SRR19973063.4636185/1‑150 (MQ=60)
                                                                           CCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAG                                                                          <  SRR19973063.1174548/150‑1 (MQ=60)
                                                                            CAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGT                                                                         <  SRR19973063.1440234/150‑1 (MQ=60)
                                                                            CAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGT                                                                         <  SRR19973063.4342176/150‑1 (MQ=60)
                                                                             AGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTG                                                                        <  SRR19973063.4684209/150‑1 (MQ=60)
                                                                             AGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTG                                                                        <  SRR19973063.2633346/150‑1 (MQ=60)
                                                                             AGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTG                                                                        <  SRR19973063.4684276/150‑1 (MQ=60)
                                                                              GTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGA                                                                       <  SRR19973063.791735/150‑1 (MQ=60)
                                                                                   TTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTG                                                                  <  SRR19973063.299703/150‑1 (MQ=60)
                                                                                       CATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCACCTGGGTGAGATAACACAAATGCCTTATACTGACGGGTTTCATAAAATTTTTGAGGGAAATATATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTTACTT                                                              >  SRR19973063.4620869/1‑150 (MQ=60)
                                                                                        ATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTT                                                             <  SRR19973063.4735600/150‑1 (MQ=60)
                                                                                           GGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATT                                                          <  SRR19973063.4178149/150‑1 (MQ=60)
                                                                                           GGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATT                                                          <  SRR19973063.4178151/150‑1 (MQ=60)
                                                                                            GTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTA                                                         <  SRR19973063.4147799/150‑1 (MQ=60)
                                                                                             TTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTAT                                                        <  SRR19973063.4162148/150‑1 (MQ=60)
                                                                                             TTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTAT                                                        <  SRR19973063.4162141/150‑1 (MQ=60)
                                                                                              TCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATT                                                       <  SRR19973063.2415844/150‑1 (MQ=60)
                                                                                               CCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTC                                                      <  SRR19973063.2729637/150‑1 (MQ=60)
                                                                                               CCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTC                                                      <  SRR19973063.1946726/150‑1 (MQ=60)
                                                                                                CGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCA                                                     <  SRR19973063.4023132/150‑1 (MQ=60)
                                                                                                 GGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTAATCAAATTTTGGAGGGAAATAGATAAATGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCAT                                                    >  SRR19973063.4303982/1‑150 (MQ=60)
                                                                                                 GGCCGTTTGTTCTTTAACCCCCCCACCAGGGTGAGATAACACAATTTCCTTATACTGACGGGTTTAATAAAAATTTGGATGGAAATATATAACTGCAGACAAAGAGATGGTTGGATTATTTCTGTTAGTGAAGCTGACTTTATTATTAAT                                                    >  SRR19973063.3971772/1‑150 (MQ=60)
                                                                                                      TTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTC                                               >  SRR19973063.238881/1‑150 (MQ=60)
                                                                                                       TTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCC                                              >  SRR19973063.4176043/1‑150 (MQ=60)
                                                                                                        TGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCG                                             <  SRR19973063.1915696/150‑1 (MQ=60)
                                                                                                          TTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTT                                           >  SRR19973063.896947/1‑150 (MQ=60)
                                                                                                             TTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCT                                        <  SRR19973063.4329900/150‑1 (MQ=60)
                                                                                                              TTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTC                                       >  SRR19973063.4562366/1‑150 (MQ=60)
                                                                                                              TTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGT                                                                                                                                           <  SRR19973063.1898693/50‑1 (MQ=60)
                                                                                                              TTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGT                                                                                                                                           <  SRR19973063.1898790/50‑1 (MQ=60)
                                                                                                               TAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCA                                      <  SRR19973063.135369/150‑1 (MQ=60)
                                                                                                                AACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCAT                                     >  SRR19973063.2385307/1‑150 (MQ=60)
                                                                                                                AACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCAT                                     >  SRR19973063.4941405/1‑150 (MQ=60)
                                                                                                                 ACGCCCCCACCTGGGTGAGATAACACAAGTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATG                                    <  SRR19973063.4381644/150‑1 (MQ=60)
                                                                                                                    CCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAG                                 >  SRR19973063.2602538/1‑150 (MQ=60)
                                                                                                                      CCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTT                               <  SRR19973063.1265136/150‑1 (MQ=60)
                                                                                                                       CCCCCTGGGTGAGATAACACACTTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTG                              <  SRR19973063.2623568/150‑1 (MQ=60)
                                                                                                                        CACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGT                             >  SRR19973063.3741198/1‑150 (MQ=60)
                                                                                                                         ACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTC                            <  SRR19973063.3716460/150‑1 (MQ=60)
                                                                                                                         ACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTC                            <  SRR19973063.5086309/150‑1 (MQ=60)
                                                                                                                         ACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTC                            <  SRR19973063.1490498/150‑1 (MQ=60)
                                                                                                                          CCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCG                           >  SRR19973063.4886763/1‑150 (MQ=60)
                                                                                                                             GGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTT                        >  SRR19973063.125022/1‑150 (MQ=60)
                                                                                                                               GTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCG                      >  SRR19973063.3517361/1‑150 (MQ=60)
                                                                                                                                  AGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTT                   <  SRR19973063.3402533/150‑1 (MQ=60)
                                                                                                                                  AGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTT                   <  SRR19973063.4681389/150‑1 (MQ=60)
                                                                                                                                  AGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTT                   >  SRR19973063.3686132/1‑150 (MQ=60)
                                                                                                                                  AGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTT                   <  SRR19973063.3320127/150‑1 (MQ=60)
                                                                                                                                        CACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGG             >  SRR19973063.4548769/1‑150 (MQ=60)
                                                                                                                                        CACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGG             >  SRR19973063.203750/1‑150 (MQ=60)
                                                                                                                                           AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTG          <  SRR19973063.5121313/150‑1 (MQ=60)
                                                                                                                                           AATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTG          <  SRR19973063.4534234/150‑1 (MQ=60)
                                                                                                                                            ATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGG         <  SRR19973063.5158093/150‑1 (MQ=60)
                                                                                                                                             TTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGT        >  SRR19973063.5158691/1‑150 (MQ=60)
                                                                                                                                             TTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGT        >  SRR19973063.186336/1‑150 (MQ=60)
                                                                                                                                              TGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTG       >  SRR19973063.5129810/1‑150 (MQ=60)
                                                                                                                                              TGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTG       >  SRR19973063.5129811/1‑150 (MQ=60)
                                                                                                                                                 CTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGATA    <  SRR19973063.1334423/150‑1 (MQ=60)
                                                                                                                                                   TATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGATATC  >  SRR19973063.3840997/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
CTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGATATC  >  CP009273/1385861‑1386157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑