Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,386,008 G→T 100% Q35K (CAG→AAG)  ycjY ← S9 homolog non‑peptidase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,386,0080GT99.6% 909.7 / ‑4.2 260Q35K (CAG→AAG) ycjYS9 homolog non‑peptidase family protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (106/152);  total (108/152)
Fisher's exact test for biased strand distribution p-value = 4.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 260 total aligned reads displayed.

CTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGAT  >  CP009273/1385861‑1386154
                                                                                                                                                   |                                                                                                                                                  
cTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATTAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTAt                                                                                                                                                  <  2:3962405/150‑1 (MQ=255)
cTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTAt                                                                                                                                                  >  1:1724157/1‑150 (MQ=255)
  aaCTGTCGCGGCTCGCCTCCACTTTCTCCTTGATAAGATGCTTCATAAGCTATATTGACAAATCCCTTTTCCGACAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACATGGGTGAGATATCACATTTGCATTATAc                                                                                                                                                <  1:1016892/150‑1 (MQ=255)
    cTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTg                                                                                                                                              <  2:4114376/150‑1 (MQ=255)
      gACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGAc                                                                                                                                            <  1:760890/150‑1 (MQ=255)
           ggCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACCCAATTGCCTTATACTGACGGGtt                                                                                                                                       >  1:238000/1‑150 (MQ=255)
            gCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGttt                                                                                                                                      <  1:782896/150‑1 (MQ=255)
               cgccgcCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTtcat                                                                                                                                   <  2:1469553/150‑1 (MQ=255)
                   gccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCaaa                                                                                                                               <  2:433504/150‑1 (MQ=255)
                   gccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGGTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTGTCATCaaa                                                                                                                               >  2:3028650/1‑150 (MQ=255)
                     cACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAAtt                                                                                                                             <  2:2713144/150‑1 (MQ=255)
                     cACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAAtt                                                                                                                             >  1:295093/1‑150 (MQ=255)
                       cTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAAtttt                                                                                                                           <  1:1339644/150‑1 (MQ=255)
                         ttCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGGTCTTTAACGCCCCCACCGGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTgg                                                                                                                         >  1:3272537/1‑150 (MQ=255)
                           cACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAg                                                                                                                       <  2:2586704/150‑1 (MQ=255)
                           cACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAg                                                                                                                       >  2:1872954/1‑150 (MQ=255)
                               ttGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGaa                                                                                                                   <  1:804057/150‑1 (MQ=255)
                                   tAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAg                                                                                                               <  2:870496/150‑1 (MQ=255)
                                    aaGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGa                                                                                                              <  1:1351249/150‑1 (MQ=255)
                                     aGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGAt                                                                                                             <  2:2883993/150‑1 (MQ=255)
                                        tGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCAc                                                                                                          <  1:701186/150‑1 (MQ=255)
                                            tCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCa                                                                                                      <  2:422888/150‑1 (MQ=255)
                                              aTAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGa                                                                                                    <  2:2484752/150‑1 (MQ=255)
                                                aaGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACa                                                                                                  <  2:2726389/150‑1 (MQ=255)
                                                aaGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAAAACAATTGCCTTATACTGACGGGTTTCATAAAAATTTTGAGGGAAATAGATCACTGCAGACa                                                                                                  >  2:2897282/1‑150 (MQ=255)
                                                   cAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAag                                                                                               <  2:3517449/150‑1 (MQ=255)
                                                          gACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGt                                                                                        <  1:3828625/150‑1 (MQ=255)
                                                           aCAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTAACGCCCCCACCTGGGTGTGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTTGAGGGAAATTGATCACAGAAGACAAAGAGATGGtt                                                                                       >  2:1183876/1‑150 (MQ=255)
                                                           aCAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGtt                                                                                       <  2:2416869/150‑1 (MQ=255)
                                                            cAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTg                                                                                      >  2:3950249/1‑150 (MQ=255)
                                                            cAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGGTg                                                                                      >  2:2304649/1‑150 (MQ=255)
                                                             aaaTCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTgg                                                                                     >  2:1991640/1‑150 (MQ=255)
                                                             aaaTCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAAACCCCCCCCCAGGGTGAGATAACACAAATGCCTTATACAGACGGGTTTAATAAAATTTTTGGGGGAAATAGATAACAGCAGACAAAGAGAGTGTTgg                                                                                     >  1:1495329/1‑150 (MQ=255)
                                                              aaTCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGa                                                                                    <  1:3798297/150‑1 (MQ=255)
                                                              aaTCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGa                                                                                    <  1:1077776/150‑1 (MQ=255)
                                                               aTCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTTTTCTTTAAAGCCCCCCCCTGGGTGAGGTAAAACAATTGCCGTATACTTACGGGTTTCATCAAAATTTTGAGGGTAAAAGATAAAGGCAGACCAAGAGATGGTTggtt                                                                                   >  1:1423219/1‑148 (MQ=255)
                                                                 cccTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGatta                                                                                 <  1:1899876/150‑1 (MQ=255)
                                                                   cTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTAAAGCAACCACCTGGGTGATATAAAACAATTTCAATATAATTAAGGGTTTAATAAAATTTTTGGTTGAAATATATAACTTCAGAAAAAGTTATGGGTGGtttatt                                                                               >  1:3671264/1‑150 (MQ=255)
                                                                    ttttCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTg                                                                              >  2:1617588/1‑150 (MQ=255)
                                                                     tttCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACCAAGAGATGGTTGGTTTATTGc                                                                             >  2:3774433/1‑150 (MQ=255)
                                                                      ttCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCt                                                                            <  1:2603925/150‑1 (MQ=255)
                                                                         cGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTTACCCCCCCACCAGGGTGAGATAACACAATAGCCTTATAAATAAGGGTTTAATCAAAATTTTGAGGGAAATATATAAATGCAGACAAAGAGATGGTTGTGTTATTTCTGtt                                                                         >  1:2846060/1‑150 (MQ=255)
                                                                         cGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAAATGCCTTATACTGACGGGTTTCATCAAATTTTTGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGGTGtt                                                                         >  1:930579/1‑150 (MQ=255)
                                                                          gCCAGTTTTTTGTCATAGGTTCCGGCGGTTTGTTCTTTTACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGTGGGTAATAGATCACTGCAGACAAAGTGATGGTTGGATTTTTGCTGTTa                                                                        <  2:3404082/150‑1 (MQ=255)
                                                                           ccAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATTGTTGGATTATTGCTGTTAg                                                                       <  2:1911754/150‑1 (MQ=255)
                                                                               ttttttGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGaa                                                                   <  1:1187912/150‑1 (MQ=255)
                                                                               ttttttGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGaa                                                                   <  1:2109200/150‑1 (MQ=255)
                                                                                tttttGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAg                                                                  <  2:1793423/150‑1 (MQ=255)
                                                                                   ttGGCATAGGTTCCGGCCGTTTTTTATTTAACGCCCCCACCTGGGTGAGATAACACAAATGCCTTATACAGACGGGTTTAATCAAATTTTGGAGGGAAATATATCACAGCAGACAAAGAGATGGTTTGATTATTGCTGTTAGTGAAGCTg                                                               >  2:3605150/1‑150 (MQ=255)
                                                                                     ggCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGAc                                                             <  2:3307202/150‑1 (MQ=255)
                                                                                     ggCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGAc                                                             <  1:205061/150‑1 (MQ=255)
                                                                                     ggCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGAc                                                             <  1:3566123/150‑1 (MQ=255)
                                                                                         tAGGTTCCGGCCGTTTTTTCTTTAAAGCCCCCACCTGGGTGAGATAACACAATTGCCGTATACTTACGGGTTTCATCAAATTTTGGAGGGAAATATATCACTGCAGACAAAGAGATTGTTGGATTATTGCTGTTAGTGAAGCTGAATtta                                                         >  2:142116/1‑150 (MQ=255)
                                                                                          aGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAAAAGATCACAGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAATCTGACTttat                                                        >  2:1007437/1‑150 (MQ=255)
                                                                                           ggTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTTCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACattatt                                                       >  2:3473269/1‑150 (MQ=255)
                                                                                           ggTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACACAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTttatt                                                       <  2:2848563/150‑1 (MQ=255)
                                                                                           ggTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTttatt                                                       <  1:3219529/150‑1 (MQ=255)
                                                                                              tCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTattatt                                                    <  1:930335/150‑1 (MQ=255)
                                                                                               ccGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtc                                                   <  2:493964/150‑1 (MQ=255)
                                                                                               ccGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtc                                                   <  1:3886864/150‑1 (MQ=255)
                                                                                                  gCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtcatc                                                <  1:3840065/150‑1 (MQ=255)
                                                                                                   ccGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtcatca                                               >  2:1280585/1‑150 (MQ=255)
                                                                                                    cGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGTTTATTGCTGTTAGTGAAGCTGACTTTATTATTcatcat                                              >  2:895052/1‑150 (MQ=255)
                                                                                                    cGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTcatcat                                              >  2:3537453/1‑150 (MQ=255)
                                                                                                     gTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCAtt                                             <  2:774652/150‑1 (MQ=255)
                                                                                                     gTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCAtt                                             >  2:720175/1‑150 (MQ=255)
                                                                                                     gTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCAtt                                             >  2:596130/1‑150 (MQ=255)
                                                                                                     gTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCAtt                                             <  1:219653/150‑1 (MQ=255)
                                                                                                       ttGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTcc                                           <  2:1810031/150‑1 (MQ=255)
                                                                                                          ttCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGtt                                        <  1:830901/150‑1 (MQ=255)
                                                                                                            cTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTcc                                      <  2:220454/150‑1 (MQ=255)
                                                                                                             tttAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCt                                     <  2:670750/150‑1 (MQ=255)
                                                                                                             tttAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCt                                     <  2:3607890/150‑1 (MQ=255)
                                                                                                             tttAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCt                                     <  1:4118427/150‑1 (MQ=255)
                                                                                                                 aCGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATg                                 >  1:1287766/1‑150 (MQ=255)
                                                                                                                  cGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATgg                                <  1:3165417/150‑1 (MQ=255)
                                                                                                                   gCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGa                               <  2:1586136/150‑1 (MQ=255)
                                                                                                                     ccccACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGt                             <  2:4147704/150‑1 (MQ=255)
                                                                                                                      cccACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTTGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGtt                            <  2:2945090/150‑1 (MQ=255)
                                                                                                                        cACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGt                          >  2:1019660/1‑150 (MQ=255)
                                                                                                                        cACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGt                          >  1:2300343/1‑150 (MQ=255)
                                                                                                                         aCCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTc                         <  2:4010129/150‑1 (MQ=255)
                                                                                                                           cTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCgg                       <  1:1820465/150‑1 (MQ=255)
                                                                                                                           cTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCgg                       >  2:3878388/1‑150 (MQ=255)
                                                                                                                             gggTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGtt                     >  1:1538734/1‑150 (MQ=255)
                                                                                                                             gggTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGtt                     >  2:1504219/1‑150 (MQ=255)
                                                                                                                              ggTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTc                    <  1:725592/150‑1 (MQ=255)
                                                                                                                              ggTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTc                    >  1:439245/1‑150 (MQ=255)
                                                                                                                               gTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCg                   <  2:3323644/150‑1 (MQ=255)
                                                                                                                                     tAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTaa             <  2:2806731/150‑1 (MQ=255)
                                                                                                                                      aaCACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAAc            >  1:1292061/1‑150 (MQ=255)
                                                                                                                                      aaCACAAGTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAAc            <  1:1031415/150‑1 (MQ=255)
                                                                                                                                        cacaATTGCCTTATACTGAAGGGTTTCATCAAATTTTGGAGGGAAATATATCACTGCAGAAAAAGAGATGGTTTTATTATTGCTGTTAGTTAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCTTTTTAACgg          <  2:1118524/150‑1 (MQ=255)
                                                                                                                                          caATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGtt        <  2:3281411/150‑1 (MQ=255)
                                                                                                                                            aTTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTgg      <  1:2778264/150‑1 (MQ=255)
                                                                                                                                            aTTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTgg      <  1:3464681/150‑1 (MQ=255)
                                                                                                                                             ttGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGt     <  1:3130389/150‑1 (MQ=255)
                                                                                                                                               gCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGa   >  1:2482528/1‑150 (MQ=255)
                                                                                                                                                ccTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGat  >  1:1217671/1‑150 (MQ=255)
                                                                                                                                                ccTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGat  >  1:493557/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGAT  >  CP009273/1385861‑1386154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGATA  >  CP009273/1385861‑1386155
                                                                                                                                                   |                                                                                                                                                   
CTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTAT                                                                                                                                                   >  SRR19973074.1717930/1‑150 (MQ=60)
CTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTAT                                                                                                                                                   >  SRR19973074.1727355/1‑150 (MQ=60)
  AACTGTCGCGGCTCGCCTCCACTTTCTCCTTGATAAGATGCTTCATAAGCTATATTGACAAATCCCTTTTCCGACAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACATGGGTGAGATATCACATTTGCATTATAC                                                                                                                                                 <  SRR19973074.1018782/150‑1 (MQ=60)
  AACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTTCCTTATAC                                                                                                                                                 >  SRR19973074.2645079/1‑150 (MQ=60)
  AACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATAC                                                                                                                                                 <  SRR19973074.136550/150‑1 (MQ=60)
   ACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACT                                                                                                                                                <  SRR19973074.1204462/150‑1 (MQ=60)
      GACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGAC                                                                                                                                             <  SRR19973074.2121854/150‑1 (MQ=60)
      GACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGAC                                                                                                                                             <  SRR19973074.762339/150‑1 (MQ=60)
           GGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACCCAATTGCCTTATACTGACGGGTT                                                                                                                                        >  SRR19973074.238482/1‑150 (MQ=60)
            GCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTT                                                                                                                                       <  SRR19973074.1178905/150‑1 (MQ=60)
            GCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTT                                                                                                                                       <  SRR19973074.784405/150‑1 (MQ=60)
              TCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCACCTGGGTGAGATAACACAAATGCCGTATACTGACGGGTTTAA                                                                                                                                     >  SRR19973074.135929/1‑150 (MQ=60)
              TCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCACCAGGGGGAGATAAAACAATTGCCTTATACTGACGGGTTTCA                                                                                                                                     >  SRR19973074.1527125/1‑150 (MQ=60)
                 CCGCCACTTTCACCTTGATAAGATGCGTCATAATCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATATCACAATTGCCTTATACTGACGGGTTTCATCA                                                                                                                                  <  SRR19973074.1274376/150‑1 (MQ=60)
                 CCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCA                                                                                                                                  <  SRR19973074.1274653/150‑1 (MQ=60)
                 CCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTATTTAAAGCCCCCACCAGGGTGAGATAAAACAATTGCCTTATACTGACGGGTTTCATCA                                                                                                                                  >  SRR19973074.831554/1‑150 (MQ=60)
                  CGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAA                                                                                                                                 <  SRR19973074.923420/150‑1 (MQ=60)
                     CACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATT                                                                                                                              >  SRR19973074.2424181/1‑150 (MQ=60)
                     CACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATT                                                                                                                              <  SRR19973074.4129680/150‑1 (MQ=60)
                     CACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATT                                                                                                                              >  SRR19973074.295641/1‑150 (MQ=60)
                       CTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTT                                                                                                                            <  SRR19973074.1342119/150‑1 (MQ=60)
                       CTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTT                                                                                                                            <  SRR19973074.1342147/150‑1 (MQ=60)
                         TTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGGTCTTTAACGCCCCCACCGGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGG                                                                                                                          >  SRR19973074.3278604/1‑150 (MQ=60)
                              CTTGATAAGGTGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTTTTATTTAAAGCCCCCACCAGGGTGAGATAAAACAAATGCCTTATACTGACGGGTTTAATAAAATTTTGGTGGGA                                                                                                                     >  SRR19973074.405446/1‑150 (MQ=60)
                               TTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAA                                                                                                                    <  SRR19973074.805601/150‑1 (MQ=60)
                               TTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAA                                                                                                                    <  SRR19973074.805554/150‑1 (MQ=60)
                                TGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCGGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAA                                                                                                                   >  SRR19973074.2380773/1‑150 (MQ=60)
                                   TAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAG                                                                                                                >  SRR19973074.187327/1‑150 (MQ=60)
                                   TAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAG                                                                                                                <  SRR19973074.2364094/150‑1 (MQ=60)
                                   TAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTATTTAACGCCCCCACCAGGGTGAGATAACACAATTGCCGTATACTGACGGGTTTCATCAAAATTTGGTGGGAAATAG                                                                                                                >  SRR19973074.714072/1‑150 (MQ=60)
                                   TAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTTAAGCCCCCACCTGGGTGAGATAACACAATTGCCGTATACTTACGGGTTTAATCAAATTTTTGTGGGAAATAG                                                                                                                >  SRR19973074.714067/1‑150 (MQ=60)
                                    AAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGA                                                                                                               >  SRR19973074.2227948/1‑150 (MQ=60)
                                    AAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGA                                                                                                               <  SRR19973074.1353723/150‑1 (MQ=60)
                                    AAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGA                                                                                                               <  SRR19973074.1353763/150‑1 (MQ=60)
                                     AGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGAT                                                                                                              <  SRR19973074.3232412/150‑1 (MQ=60)
                                     AGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAAAACAATTGCCTTATACTGTCGGGGTTCATCAAATTTTGGCGGGGAATAGAT                                                                                                              >  SRR19973074.1641664/1‑150 (MQ=60)
                                        TGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCAC                                                                                                           <  SRR19973074.702483/150‑1 (MQ=60)
                                            TCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCA                                                                                                       <  SRR19973074.261029/150‑1 (MQ=60)
                                             CATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAG                                                                                                      <  SRR19973074.783169/150‑1 (MQ=60)
                                                     ATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCAGGGTGAGATAAAACAATTGCCGAATAAAGACGGGGTTGATAAAATTTTGGAGGGAAATAGATCCCAGCAGACAAACAG                                                                                              >  SRR19973074.3743493/1‑150 (MQ=60)
                                                         TGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGG                                                                                          <  SRR19973074.1303079/150‑1 (MQ=60)
                                                          GACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGT                                                                                         <  SRR19973074.3835606/150‑1 (MQ=60)
                                                           ACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTT                                                                                        <  SRR19973074.489717/150‑1 (MQ=60)
                                                           ACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCACCAGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTAATCAAATTTTGGAGGGAAATATATAACTGGAGACAAAGAGATGGTT                                                                                        >  SRR19973074.1865442/1‑150 (MQ=60)
                                                            CAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAAAGCCCCCACCCGGGTGTGATAAAACAATTTCCGTATAATTACGGGGTTAATAAAATTTTTGAGGGAAATAGATCACAGCAGACAAAGAGATGGTTG                                                                                       >  SRR19973074.1053860/1‑150 (MQ=60)
                                                             AAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCACCGGGGTGAGATAACACAAATGCCATATACAGACGGGTTTCATCAAATTTTGGAGGGAAATAGAACACTGCAGACAAAGAGATGGGTGG                                                                                      >  SRR19973074.3137458/1‑150 (MQ=60)
                                                             AAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAAACCCCCCCCCAGGGTGAGATAACACAAATGCCTTATACAGACGGGTTTAATAAAATTTTTGGGGGAAATAGATAACAGCAGACAAAGAGAGTGTTGG                                                                                      >  SRR19973074.1498149/1‑150 (MQ=60)
                                                              AATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGA                                                                                     <  SRR19973074.1079785/150‑1 (MQ=60)
                                                              AATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGA                                                                                     <  SRR19973074.3805254/150‑1 (MQ=60)
                                                              AATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGA                                                                                     <  SRR19973074.3805336/150‑1 (MQ=60)
                                                               ATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTAACGCCCCCCCCGGGGTGTGATAAAACAAATGCCGTATAATGACGGGTTTAATAAAAATTTGGAGGGAAATAGATAACTGCAGACAAAGAGATGGTTGGAT                                                                                    >  SRR19973074.1425856/1‑150 (MQ=60)
                                                               ATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTTTTCTTTAAAGCCCCCCCCTGGGTGAGGTAAAACAATTGCCGTATACTTACGGGTTTCATCAAAATTTTGAGGGTAAAAGATAAAGGCAGACCAAGAGATGGTTGGTT                                                                                    >  SRR19973074.1425852/1‑150 (MQ=58)
                                                                 CCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTA                                                                                  <  SRR19973074.1903518/150‑1 (MQ=60)
                                                                 CCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTA                                                                                  <  SRR19973074.1903517/150‑1 (MQ=60)
                                                                 CCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATAACTGCAGACAAAGAGATGGTTGGATTA                                                                                  >  SRR19973074.632114/1‑150 (MQ=60)
                                                                   CTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATT                                                                                <  SRR19973074.3603153/150‑1 (MQ=60)
                                                                      TTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCT                                                                             <  SRR19973074.2608798/150‑1 (MQ=60)
                                                                      TTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCT                                                                             <  SRR19973074.2882143/150‑1 (MQ=60)
                                                                      TTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCT                                                                             <  SRR19973074.106294/150‑1 (MQ=60)
                                                                      TTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACAGCAGACAAAGAGATGGTTGGATTATTGCT                                                                             >  SRR19973074.2413271/1‑150 (MQ=60)
                                                                      TTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCGGGGTGAGATAACACAATTGCCTTATACTGAAGGGTTTCATCAAATTTTTGAGGGGAAAAGATCACGGCAGACCAAGAGAGGGGTGGGTTATTTCT                                                                             >  SRR19973074.2815673/1‑150 (MQ=60)
                                                                        CCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGT                                                                           <  SRR19973074.3487233/150‑1 (MQ=60)
                                                                         CGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTTACCCCCCCACCAGGGTGAGATAACACAATAGCCTTATAAATAAGGGTTTAATCAAAATTTTGAGGGAAATATATAAATGCAGACAAAGAGATGGTTGTGTTATTTCTGTT                                                                          >  SRR19973074.2851336/1‑150 (MQ=48)
                                                                         CGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAAATGCCTTATACTGACGGGTTTCATCAAATTTTTGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGGTGTT                                                                          >  SRR19973074.932347/1‑150 (MQ=60)
                                                                           CCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTTCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAG                                                                        <  SRR19973074.2987692/150‑1 (MQ=60)
                                                                             AGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTAATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTTCTGTGAGTG                                                                      >  SRR19973074.1956751/1‑150 (MQ=60)
                                                                               TTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAA                                                                    <  SRR19973074.1190178/150‑1 (MQ=60)
                                                                               TTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAA                                                                    <  SRR19973074.2113157/150‑1 (MQ=60)
                                                                                   TTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCAGGGTGAGATAACACAATTGCCGTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTG                                                                >  SRR19973074.3357120/1‑150 (MQ=60)
                                                                                    TGGCATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCACCGGGGTGAGATAACACAATTGCCTTATACAGACGGGTTTCATCAAAATTTGGAGGGTAATATATAACGGCAGACAAAGAGATGGTTGGGTTATTTCTGTTAGTGAAGCGGA                                                               >  SRR19973074.592766/1‑150 (MQ=60)
                                                                                     GGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGAC                                                              <  SRR19973074.205491/150‑1 (MQ=60)
                                                                                     GGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGAC                                                              <  SRR19973074.3572701/150‑1 (MQ=60)
                                                                                           GGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATT                                                        <  SRR19973074.3225477/150‑1 (MQ=60)
                                                                                             TTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTAT                                                      <  SRR19973074.2924059/150‑1 (MQ=60)
                                                                                              TCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATT                                                     <  SRR19973074.932105/150‑1 (MQ=60)
                                                                                              TCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATT                                                     <  SRR19973074.932103/150‑1 (MQ=60)
                                                                                               CCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTC                                                    <  SRR19973074.3893986/150‑1 (MQ=60)
                                                                                               CCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTC                                                    <  SRR19973074.3844391/150‑1 (MQ=60)
                                                                                               CCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTC                                                    <  SRR19973074.3844385/150‑1 (MQ=60)
                                                                                                CGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCA                                                   >  SRR19973074.957284/1‑150 (MQ=60)
                                                                                                 GGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCAT                                                  >  SRR19973074.3437545/1‑150 (MQ=60)
                                                                                                 GGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCAT                                                  <  SRR19973074.1764993/150‑1 (MQ=60)
                                                                                                  GCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATC                                                 <  SRR19973074.3847060/150‑1 (MQ=60)
                                                                                                  GCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATC                                                 <  SRR19973074.3847128/150‑1 (MQ=60)
                                                                                                     GTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATT                                              <  SRR19973074.220109/150‑1 (MQ=60)
                                                                                                      TTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTC                                             <  SRR19973074.1497301/150‑1 (MQ=60)
                                                                                                          TTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTT                                         <  SRR19973074.832499/150‑1 (MQ=60)
                                                                                                          TTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTT                                         <  SRR19973074.832498/150‑1 (MQ=60)
                                                                                                          TTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTT                                         <  SRR19973074.832470/150‑1 (MQ=60)
                                                                                                             TTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCT                                      <  SRR19973074.4126025/150‑1 (MQ=60)
                                                                                                                AACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCAT                                   >  SRR19973074.75835/1‑150 (MQ=60)
                                                                                                                 ACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATG                                  <  SRR19973074.3546347/150‑1 (MQ=60)
                                                                                                                 ACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATG                                  >  SRR19973074.1290210/1‑150 (MQ=60)
                                                                                                                 ACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATG                                  >  SRR19973074.1290225/1‑150 (MQ=60)
                                                                                                                  CGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGG                                 <  SRR19973074.3171239/150‑1 (MQ=60)
                                                                                                                        CACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGT                           >  SRR19973074.2304646/1‑150 (MQ=60)
                                                                                                                           CTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGG                        <  SRR19973074.1823934/150‑1 (MQ=60)
                                                                                                                             GGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTT                      >  SRR19973074.1541656/1‑150 (MQ=60)
                                                                                                                              GGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTC                     <  SRR19973074.726954/150‑1 (MQ=60)
                                                                                                                              GGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTC                     >  SRR19973074.440094/1‑150 (MQ=60)
                                                                                                                               GTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCG                    >  SRR19973074.2162121/1‑150 (MQ=60)
                                                                                                                               GTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCG                    >  SRR19973074.77744/1‑150 (MQ=60)
                                                                                                                                   GATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTT                <  SRR19973074.4126480/150‑1 (MQ=60)
                                                                                                                                      AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAAC             <  SRR19973074.1033267/150‑1 (MQ=60)
                                                                                                                                      AACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAAC             >  SRR19973074.1294512/1‑150 (MQ=60)
                                                                                                                                      AACACAAGTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAAC             <  SRR19973074.1033318/150‑1 (MQ=60)
                                                                                                                                       ACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACG            <  SRR19973074.2077026/150‑1 (MQ=60)
                                                                                                                                            ATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGG       >  SRR19973074.2986146/1‑150 (MQ=60)
                                                                                                                                            ATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGG       <  SRR19973074.3471048/150‑1 (MQ=60)
                                                                                                                                            ATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGG       <  SRR19973074.2783359/150‑1 (MQ=60)
                                                                                                                                             TTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGT      <  SRR19973074.3136119/150‑1 (MQ=60)
                                                                                                                                             TTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGT      <  SRR19973074.3200139/150‑1 (MQ=60)
                                                                                                                                             TTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGT      <  SRR19973074.3200177/150‑1 (MQ=60)
                                                                                                                                               GCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGA    >  SRR19973074.2487132/1‑150 (MQ=60)
                                                                                                                                               GCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGA    >  SRR19973074.2487246/1‑150 (MQ=60)
                                                                                                                                                CCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGAT   >  SRR19973074.3685072/1‑150 (MQ=60)
                                                                                                                                                CCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGAT   >  SRR19973074.1220007/1‑150 (MQ=60)
                                                                                                                                                CCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGAT   >  SRR19973074.1220004/1‑150 (MQ=60)
                                                                                                                                                CCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGAT   >  SRR19973074.494549/1‑150 (MQ=60)
                                                                                                                                                 CTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGATA  >  SRR19973074.600391/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                   
CTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGATA  >  CP009273/1385861‑1386155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑