Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,303,607 A→C 8.9% L93R (CTG→CGG)  ada ← fused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,303,6070AC 8.9% 687.8 / 12.2 286L93R (CTG→CGG) adafused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (101/147);  new base C (31/4);  total (132/154)
Fisher's exact test for biased strand distribution p-value = 7.43e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 297 total aligned reads displayed.

ATTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCG  >  CP009273/2303458‑2303756
                                                                                                                                                     |                                                                                                                                                     
aTTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCcagca                                                                                                                                                       >  2:3036882/1‑150 (MQ=255)
   cgcgCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTc                                                                                                                                                    <  1:3641813/150‑1 (MQ=255)
   cgcgCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTc                                                                                                                                                    <  2:664035/150‑1 (MQ=255)
   cgcgCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTc                                                                                                                                                    <  2:102647/150‑1 (MQ=255)
      gcAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTTGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTGCCTGTTCCCGCAGTCGac                                                                                                                                                 >  1:894484/1‑150 (MQ=255)
        aaaCGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGacac                                                                                                                                               <  2:2244208/150‑1 (MQ=255)
           cGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACAcgcg                                                                                                                                            <  1:1580479/150‑1 (MQ=255)
              cGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTgg                                                                                                                                         <  2:2986687/150‑1 (MQ=255)
               gAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTggg                                                                                                                                        <  1:2073035/150‑1 (MQ=255)
                  cgcgcCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTGCTTTTGCAGCCGTCGACACGGGTGGGTGa                                                                                                                                     >  1:969830/1‑150 (MQ=255)
                   gcgcCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGAt                                                                                                                                    <  1:3036869/150‑1 (MQ=255)
                    cgcCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGAtt                                                                                                                                   <  1:1806374/150‑1 (MQ=255)
                        aGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTAt                                                                                                                               <  1:4530802/150‑1 (MQ=255)
                         ggCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCCGTCGACACGCGTGGGTGATTTTATc                                                                                                                              >  2:3879893/1‑150 (MQ=255)
                         ggCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATc                                                                                                                              <  1:4395318/150‑1 (MQ=255)
                         ggCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATc                                                                                                                              <  2:651519/150‑1 (MQ=255)
                         ggCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATc                                                                                                                              <  1:1647568/150‑1 (MQ=255)
                           ccTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCa                                                                                                                            >  2:3604418/1‑150 (MQ=255)
                            cTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATccca                                                                                                                           >  2:3652858/1‑148 (MQ=255)
                            cTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCaa                                                                                                                           >  2:2775429/1‑150 (MQ=255)
                             tGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAAc                                                                                                                          <  2:1337222/150‑1 (MQ=255)
                               ttGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCg                                                                                                                        <  1:3888206/150‑1 (MQ=255)
                                 gCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGAt                                                                                                                      <  2:557121/150‑1 (MQ=255)
                                  ccAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATg                                                                                                                     <  2:1263551/150‑1 (MQ=255)
                                    aGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGtt                                                                                                                   <  2:3019206/150‑1 (MQ=255)
                                    aGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGtt                                                                                                                   <  1:645287/150‑1 (MQ=255)
                                    aGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGtt                                                                                                                   <  1:1209667/150‑1 (MQ=255)
                                    aGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGtt                                                                                                                   <  1:1967378/150‑1 (MQ=255)
                                     ggCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTg                                                                                                                  <  2:1224210/150‑1 (MQ=255)
                                         ttAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTgg                                                                                                              <  2:462538/150‑1 (MQ=255)
                                          tAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTggg                                                                                                             <  2:3073523/150‑1 (MQ=255)
                                            ggCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCa                                                                                                           <  1:1792257/150‑1 (MQ=255)
                                            ggCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCa                                                                                                           <  1:4069506/150‑1 (MQ=255)
                                               gTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTg                                                                                                        >  2:2516348/1‑150 (MQ=255)
                                               gTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTg                                                                                                        <  2:3966664/150‑1 (MQ=255)
                                               gTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTg                                                                                                        >  2:4380732/1‑150 (MQ=255)
                                                tCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTACACCAGTCGACACCCGGGGGTGATTTTATACAACCGATGTTGCTGGGCCtttg                                                                                                       >  1:3185691/1‑148 (MQ=255)
                                                 cATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGc                                                                                                      <  1:1027929/150‑1 (MQ=255)
                                                 cATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTACTGTTACAGCCGTCGACACGCGTGGGTGATTTTATACAACCGATGTTTCTGGGCATTGGc                                                                                                      >  1:1329774/1‑150 (MQ=255)
                                                   ttCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCtt                                                                                                    >  2:2628432/1‑150 (MQ=255)
                                                    tCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCttt                                                                                                   <  2:1726557/150‑1 (MQ=255)
                                                        gTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTTGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACAAGCGTGGGTGATTTTATACAACCGATGTTTCTGGGCATTTGCtttttat                                                                                               >  2:1100269/1‑148 (MQ=255)
                                                                tttAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACa                                                                                       <  1:3361645/150‑1 (MQ=255)
                                                                  tAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAAc                                                                                     >  2:3248351/1‑150 (MQ=255)
                                                                  tAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAAc                                                                                     >  2:3184434/1‑150 (MQ=255)
                                                                  tAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCGGTTCCAGCCGTCGACACGCGTGGGTGATTTTATACAACCGATGTTGCTGGGCCTTGGCTTTTTCTGGCTGACAAc                                                                                     >  1:2537258/1‑150 (MQ=255)
                                                                   aaaCAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGGGGGTGTTTTTATCCAACCGATGTTGCTGGGCCTTGGGTTTTTATTGCTGACaaag                                                                                    >  1:819974/1‑148 (MQ=255)
                                                                     acaacCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCATCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGtt                                                                                  <  2:1980682/150‑1 (MQ=255)
                                                                     acaacCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGtt                                                                                  <  2:3931636/150‑1 (MQ=255)
                                                                     acaacCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGtt                                                                                  <  2:4466203/150‑1 (MQ=255)
                                                                      caacCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTGTCCTGTTACAGCAGTCGACACGCGTGGGTGATTTTATACAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGTCAACGttt                                                                                 >  1:3940787/1‑150 (MQ=255)
                                                                       aacCGATGTAGATGAACTGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTg                                                                                <  1:899582/150‑1 (MQ=255)
                                                                         ccGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCa                                                                              <  1:710973/150‑1 (MQ=255)
                                                                          cGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCAGTTCCAGCAGTGGACACCCGTGGGGGTTTTTATCAAACAGATGTTGGTGGGCATTGGGTTTTTATGGCTGTCAACGTTTTCAg                                                                             >  1:2732460/1‑150 (MQ=255)
                                                                            aTGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAggg                                                                           >  2:1805309/1‑150 (MQ=255)
                                                                                 gATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTGTGCTGTTACAGCCGGCGACACGCGTGGGTGATTTTATACAAACGATGTTGGTGGGGATTGGCTTTTTATGGCTGACAACGTTTGAAGGGGCGaa                                                                      >  1:1534863/1‑150 (MQ=255)
                                                                                    gAACTGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGc                                                                   <  1:1251592/150‑1 (MQ=255)
                                                                                    gAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCCGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGc                                                                   >  1:2485533/1‑150 (MQ=255)
                                                                                    gAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGc                                                                   <  1:1253075/150‑1 (MQ=255)
                                                                                    gAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGc                                                                   <  1:354330/150‑1 (MQ=255)
                                                                                     aaaTGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCCGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGcc                                                                  >  2:2065465/1‑150 (MQ=255)
                                                                                      aaTGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCa                                                                 <  2:2344166/150‑1 (MQ=255)
                                                                                      aaTGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTGTCCTGTTCCAGCCGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGccc                                                                 >  1:283398/1‑149 (MQ=255)
                                                                                       aTGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAg                                                                >  2:4522048/1‑150 (MQ=255)
                                                                                       aTGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTGTCCTGTTCCAGCCGTCGACACGCGTGGGTGATTTTATACAACCGCTGTTGCTGGGGATTGGCTTTTTGTGGCTGAAAACGGTTGGAGGGGCGAAAGcccg                                                                >  1:1248197/1‑148 (MQ=255)
                                                                                           aCTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGGTTGCAGGGGCGAAAGCCAgcggc                                                            >  2:946129/1‑150 (MQ=255)
                                                                                               aTCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTGTGCTGGTCCAGCCGTCGACACGCGTGGGTGTTTTTATACAACCGATGTTTCTGGGGATTTGCTTTTTGTGGCTGACAACGTTTTCAGGGGCGAAAGCCCgcggcgcgg                                                        >  1:1542204/1‑147 (MQ=255)
                                                                                                tCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTg                                                       >  2:3709209/1‑150 (MQ=255)
                                                                                                tCGCCACCGGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTg                                                       <  2:2417363/150‑1 (MQ=255)
                                                                                                  gCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATACAACCGATGTTTCTGGGCATTGGCGTTTTCTGGCTGACAACGTTTTCAGGGGCGAAAGCCAGCGGCGAGTGcc                                                     >  2:3804167/1‑150 (MQ=255)
                                                                                                  gCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTGTGCGGTTCCAGCCGTCGCCACGCGTGGGTGATTTTATACAACCCAGGTTTCTGGGCAGTTGCTTTTTATGGCTGACAACGTTTGCAGGGGCGAAAGCCCGCGGCGAGTGcc                                                     >  1:3459010/1‑150 (MQ=255)
                                                                                                  gCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTGTCCGGTTCCAGCCGTCGACACGCGTGGGGGCTTTTATCCAACCGATGTTGCTGGGCAGTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCCCCGGCGAGTGcc                                                     >  1:2864988/1‑150 (MQ=255)
                                                                                                     aCCTGGTCGGCTAAGGCTTCCTGCGTTACTGGCGTTTCCTGTTCCTGCAGTCGACAGGCGTGGGTTATTTTATCCAACCTATGTTGGTGGGAATTGGTTTTTTCTGGCTGACAACGTTTGCTGGGGCGAAAGCCAGCGGCGAGTGCCTCg                                                  <  1:678697/150‑1 (MQ=255)
                                                                                                       cTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCt                                                >  2:1622770/1‑150 (MQ=255)
                                                                                                             ggCTACGGCATCCCGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTTATTTTAACCAACCTATGTTGCTGGGAATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCAtt                                          <  2:4165806/150‑1 (MQ=255)
                                                                                                               cTACGGCTTCCTGCGTTACCGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCTGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTg                                        <  1:2167936/150‑1 (MQ=255)
                                                                                                                tAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATACAACCGATGTTGCTGGGCATTGGCTTTTTCTGGGTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGc                                       >  1:4283285/1‑150 (MQ=255)
                                                                                                                 aaGGCTTCCTGGGTTACTGGGGTTTCCTGTTCCAGCCGTCGACCCGCGTGGGTTATTTTATCCAACCGAGGTTGCTGGGCTTTGGCTTTTTCGGGCTGACACCGTTTGCTGGGGCTAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCg                                      <  1:4045503/150‑1 (MQ=255)
                                                                                                                    gCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAg                                   <  2:3298629/150‑1 (MQ=255)
                                                                                                                    gCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAg                                   >  2:2487024/1‑150 (MQ=255)
                                                                                                                    gCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCAATTGCGTAg                                   >  2:1499450/1‑150 (MQ=255)
                                                                                                                        ccAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAgg                               >  2:1329934/1‑150 (MQ=255)
                                                                                                                        ccAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAgg                               >  2:694589/1‑150 (MQ=255)
                                                                                                                         cAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGga                              >  2:4254092/1‑150 (MQ=255)
                                                                                                                          aGCGTTACAGGCGTTTCCAGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCAAACCGATGTTGCTGGGGATTTGCTTTTTCTGGCTGACAACCTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCGCGCTGGCAATTTCGTATAAGgag                             >  1:3933619/1‑150 (MQ=255)
                                                                                                                           gctttACCGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATGGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGgaga                            <  1:3708143/147‑1 (MQ=255)
                                                                                                                           gCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGgaga                            <  2:2169260/150‑1 (MQ=255)
                                                                                                                            cGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCTTTGGCTTTTTCTGGCTGACAACGTTTGCTGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGAc                           <  1:987150/150‑1 (MQ=255)
                                                                                                                            cGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGAc                           >  2:1390885/1‑150 (MQ=255)
                                                                                                                              ttACCGGCGTTTCCTGTTCCAGCAGTCGACACGGGTGGGTTATTTTATCCAACCGATGTTGCTGGGCTTTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGt                         <  1:4496423/150‑1 (MQ=255)
                                                                                                                                 cAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCAAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCAAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGtttt                      <  1:3770312/150‑1 (MQ=255)
                                                                                                                                       tttCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCGGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCa                <  1:2222552/150‑1 (MQ=255)
                                                                                                                                          ccTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAg             >  1:1392603/1‑150 (MQ=255)
                                                                                                                                            tGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCa           >  1:3285882/1‑150 (MQ=255)
                                                                                                                                              ttCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATg         <  2:894472/150‑1 (MQ=255)
                                                                                                                                               tCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGt        <  1:1058909/150‑1 (MQ=255)
                                                                                                                                                  agcagTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTg     >  2:3446818/1‑150 (MQ=255)
                                                                                                                                                   gcagTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTgg    >  2:528681/1‑150 (MQ=255)
                                                                                                                                                     agTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGgcg  >  2:2369551/1‑150 (MQ=255)
                                                                                                                                                     agTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGgcg  >  2:839776/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCG  >  CP009273/2303458‑2303756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A