Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,303,607 A→C 9.2% L93R (CTG→CGG)  ada ← fused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,303,6070AC 9.2% 769.3 / 14.1 334L93R (CTG→CGG) adafused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (123/167);  new base C (40/2);  total (163/171)
Fisher's exact test for biased strand distribution p-value = 9.63e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 349 total aligned reads displayed.

TTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCG  >  CP009273/2303459‑2303756
                                                                                                                                                    |                                                                                                                                                     
ttCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCcagcag                                                                                                                                                      >  2:2045204/1‑150 (MQ=255)
  cgcgCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTc                                                                                                                                                    <  1:236013/150‑1 (MQ=255)
    cgcAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCGGTTCCAGCAGTCGa                                                                                                                                                  >  1:2446571/1‑150 (MQ=255)
      cAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGaca                                                                                                                                                >  2:2532716/1‑150 (MQ=255)
        aaCGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCCGGCGACCcg                                                                                                                                              >  1:2912160/1‑150 (MQ=255)
          cGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTTTTACAGCCGTAGACAcgcg                                                                                                                                            >  1:300804/1‑150 (MQ=255)
            gCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTg                                                                                                                                          <  2:3137123/150‑1 (MQ=255)
              gAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTggg                                                                                                                                        >  1:586518/1‑150 (MQ=255)
                 cgcgcCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGa                                                                                                                                     <  1:3713460/150‑1 (MQ=255)
                   cgcCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCCGTCGACACGCGTGGGTGAtt                                                                                                                                   >  1:4602153/1‑150 (MQ=255)
                   cgcCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGAtt                                                                                                                                   <  2:1984087/150‑1 (MQ=255)
                    gcCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGAttt                                                                                                                                  <  1:3592129/150‑1 (MQ=255)
                    gcCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGAttt                                                                                                                                  <  1:1122983/150‑1 (MQ=255)
                     ccAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGAtttt                                                                                                                                 <  2:777368/150‑1 (MQ=255)
                     ccAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGAtttt                                                                                                                                 <  1:3615790/150‑1 (MQ=255)
                     ccAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAAGGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGAtttt                                                                                                                                 <  1:1202735/150‑1 (MQ=255)
                      cAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTGTCCTGTTCCAGCCGTCGACACGCGTGGGTGATTTTa                                                                                                                                >  1:3515608/1‑150 (MQ=255)
                       aGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTAt                                                                                                                               <  1:1458311/150‑1 (MQ=255)
                        ggCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTGCTGTTCCAGCCGTCGACCCGCGGGGGTGGTTTTAta                                                                                                                              >  1:4415853/1‑149 (MQ=255)
                        ggCCTGGTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATc                                                                                                                              <  1:830648/150‑1 (MQ=255)
                          ccTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCa                                                                                                                            <  2:4189/150‑1 (MQ=255)
                            tGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAAc                                                                                                                          <  2:130633/150‑1 (MQ=255)
                              ttGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCg                                                                                                                        >  2:941646/1‑150 (MQ=255)
                                   aGGCTTTAGTCGTCATTCCGGTAGTCGCTTTAAACAACCTATTTAGATGAAATGGACTCATCGCCACCTGGTCGTCTAAGTCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGAtttt                                                                                                                   <  1:3458875/150‑1 (MQ=255)
                                   aGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGGTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATACAACCGAGGtt                                                                                                                   >  2:2935407/1‑150 (MQ=255)
                                    ggCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCAGTTCCAGCCGTCGACACGCGTGGGTGATTTTATACAACCGATGTTg                                                                                                                  >  2:1097289/1‑150 (MQ=255)
                                     gCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGc                                                                                                                 >  2:2828683/1‑150 (MQ=255)
                                      cTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCt                                                                                                                <  2:4073624/150‑1 (MQ=255)
                                       tttAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGCTGACAGGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTg                                                                                                               <  1:1522679/150‑1 (MQ=255)
                                       tttAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTg                                                                                                               <  1:2482720/150‑1 (MQ=255)
                                        ttAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTGCGGTTACAGCAGTCGACACGCGTGGGTGATTTTATACAACCCCTGTTGCTgg                                                                                                              >  1:3410816/1‑150 (MQ=255)
                                           ggCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCGGTTCCACCCGGCGACACCCGTGGGTGATTTTATCCAACCGATGTTTCTGGGCa                                                                                                           >  1:2604155/1‑150 (MQ=255)
                                             cGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCAtt                                                                                                         <  2:1566210/150‑1 (MQ=255)
                                               tCATTCCTGTAGTCGCTTTAAACAACCGATGTAGATGAACTGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTgg                                                                                                       <  1:4101924/150‑1 (MQ=255)
                                               tCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTgg                                                                                                       <  2:3216766/150‑1 (MQ=255)
                                                  ttCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGGCGACACGCGTGGGTGATTTTATACAACCGATGTTGCTGGGCATTGGCtt                                                                                                    >  1:1807613/1‑150 (MQ=255)
                                                    ccGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTTTTACAGCAGTCGAACCCCGGGGGTGATTTTATACAACCCAAGTTTCGGGGCATTGGCtttt                                                                                                  >  1:1695652/1‑150 (MQ=255)
                                                    ccGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCtttt                                                                                                  <  1:4115400/150‑1 (MQ=255)
                                                     cGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGTTTTTATCCAACCGATGTTGCTGGGCATTGGCttttt                                                                                                 >  2:2855704/1‑150 (MQ=255)
                                                      ggTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACTTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTc                                                                                                <  2:1874451/150‑1 (MQ=255)
                                                          gTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGc                                                                                            >  2:4385078/1‑150 (MQ=255)
                                                             gCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGa                                                                                         >  2:4784522/1‑150 (MQ=255)
                                                             gCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGa                                                                                         <  2:2393957/150‑1 (MQ=255)
                                                                 tAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAAc                                                                                     <  1:807839/150‑1 (MQ=255)
                                                                  aaaCAACCGATTTTGATGATATGGACTCCTCGCCACCTGGTCGGCTAAGGCTTGCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACg                                                                                    <  1:3934501/150‑1 (MQ=255)
                                                                  aaaCAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACg                                                                                    <  1:4763973/150‑1 (MQ=255)
                                                                   aacaacCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGt                                                                                   >  2:3733224/1‑150 (MQ=255)
                                                                      aacCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTTGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTg                                                                                <  2:281883/150‑1 (MQ=255)
                                                                      aacCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTg                                                                                >  1:3128112/1‑150 (MQ=255)
                                                                       acCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGc                                                                               <  1:2054182/150‑1 (MQ=255)
                                                                           aTGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACCCGCGTGGGTGATTTTATCCAACCGAGGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAggg                                                                           >  2:638119/1‑150 (MQ=255)
                                                                            tGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCCGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCCTTGGCTTTTTCTGGCTGACAACGTTTGCCgggg                                                                          >  1:4685504/1‑150 (MQ=255)
                                                                            tGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAgggg                                                                          <  1:646979/150‑1 (MQ=255)
                                                                              tAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCg                                                                        <  1:1684322/150‑1 (MQ=255)
                                                                               aGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTGTCCGGTTCCAGCAGTCGACACGCGTGGGTGATTTTATACAACCGATGTTGCTGGGGATTTGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGa                                                                       >  1:1385035/1‑150 (MQ=255)
                                                                                 aTGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGGCGACACGCGTGGGGGATTTTATCCAACCGCTGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGaaa                                                                     >  2:3690965/1‑150 (MQ=255)
                                                                                     aaTGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCa                                                                 <  2:2272633/150‑1 (MQ=255)
                                                                                      aTGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAg                                                                <  1:1308024/150‑1 (MQ=255)
                                                                                              aTCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGCCACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCCTTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGt                                                        >  2:1708936/1‑150 (MQ=255)
                                                                                                cGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGc                                                      >  2:2627455/1‑150 (MQ=255)
                                                                                                cGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGc                                                      >  2:2329045/1‑150 (MQ=255)
                                                                                                 gCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCCGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTATGGCTGACAAAGTTTGCAGGGGCGAAAGCCAGCGGCGCGTGcc                                                     >  1:2110341/1‑150 (MQ=255)
                                                                                                  ccACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCCTTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCt                                                    >  2:1386665/1‑150 (MQ=255)
                                                                                                  ccACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCt                                                    >  2:3797474/1‑150 (MQ=255)
                                                                                                   cACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCGGTTCCAGCCGTCGACACCCGGGGGTGATTTTATCCAACCCATGTTGCTGGGCCTTGGCTTTTTCTGGCTGACAACGGTTGCAGGGGCGAAAGCCACCGGCGAGTGccgc                                                   >  2:818045/1‑148 (MQ=255)
                                                                                                   aaCCTGGGCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAACGCCAGCGGCGAGTGCCTc                                                   <  2:2228974/149‑1 (MQ=255)
                                                                                                      cTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCt                                                <  2:1921331/150‑1 (MQ=255)
                                                                                                       tGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCCGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTg                                               >  2:4544080/1‑150 (MQ=255)
                                                                                                        ggTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCCGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCGCGCAgg                                              >  1:3978487/1‑150 (MQ=255)
                                                                                                        ggTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCGGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCTGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTgg                                              <  2:509195/150‑1 (MQ=255)
                                                                                                          tCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCa                                            <  2:4602153/150‑1 (MQ=255)
                                                                                                          tCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCa                                            <  2:352611/150‑1 (MQ=255)
                                                                                                            ggatacggCTCCCAGCGTTACTGGCGTTTCCTGTTCCAGCATTCGACACGGGTGGGTTATTTTACCCACCCGTTGTTGGTGGGCTTTGGCTTTTTCTGGCTGAAAACGTTTGCTGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCAtt                                          <  1:184121/144‑1 (MQ=255)
                                                                                                             gCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCAttt                                         >  2:714704/1‑150 (MQ=255)
                                                                                                              cTAAGGCTTCCAGCGTTACAGGCGTGTCCGGTTACAGCCGTCGACACCCGGGGGTGGTTTTATACAACCCATGTTGCTGGGCAATTGCTTTTTCTGGCTGACAACGTTTTCAGGGGCGAAAGCCAGCGGCGAGTGCCGCGCTGGCAtttt                                        >  1:3263639/1‑149 (MQ=255)
                                                                                                               tAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGc                                       <  2:496140/150‑1 (MQ=255)
                                                                                                               tAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGc                                       >  2:3311118/1‑150 (MQ=255)
                                                                                                               tAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGc                                       >  2:3927521/1‑150 (MQ=255)
                                                                                                                aaGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCg                                      >  2:1151731/1‑150 (MQ=255)
                                                                                                                aaGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGAAACGCGTGGGTGATTTTATCCAACCGATGTTGCAGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCg                                      >  2:3952998/1‑150 (MQ=255)
                                                                                                                  ggCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTa                                    <  2:1901403/150‑1 (MQ=255)
                                                                                                                   gCTTCCAGCGTTACCGGCGTTTCCTGTTCCAGCAGTCGACACGCGGGGGTTATTTTATCCACCCGTTGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCTAACGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAg                                   <  1:1026100/150‑1 (MQ=255)
                                                                                                                   gCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCTTGGGCTTTTTCTGGCTGACACCGTTTGCTGGGGCGAAACCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAg                                   <  1:2292782/150‑1 (MQ=255)
                                                                                                                   gCTTCCAGCGTTACAGGCGTTTCCTGTACCAGCAGTCGACACGCGGGGGTGATTTTATCCAACCGATGTTGCTGGGCAGGGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAg                                   <  2:1824307/150‑1 (MQ=255)
                                                                                                                       cctgctttACTGGCGTTTCCTGTTCCAGCAGTCTACACGCGTGGGTTATTTTATCCAACCGTTGTTGCGGGGCTTTGGCTTTTTCTGGCTGACAACGTTGGCTGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAgg                               <  1:2856645/144‑1 (MQ=255)
                                                                                                                        cAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGga                              >  2:2692019/1‑150 (MQ=255)
                                                                                                                        cAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGga                              >  2:3367780/1‑150 (MQ=255)
                                                                                                                         aGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGgag                             <  2:2911308/150‑1 (MQ=255)
                                                                                                                           cGTTACAGGCGGTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGAc                           <  2:1024121/150‑1 (MQ=255)
                                                                                                                            gTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGCCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGTGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACg                          <  2:2129240/150‑1 (MQ=255)
                                                                                                                             ttACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGCGACGt                         >  2:4283088/1‑150 (MQ=255)
                                                                                                                             ttACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTATGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGt                         >  2:2970293/1‑150 (MQ=255)
                                                                                                                                 aGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTc                     >  1:2875203/1‑150 (MQ=255)
                                                                                                                                 aGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACATTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTc                     >  1:731264/1‑150 (MQ=255)
                                                                                                                                         ccTGTTCCAGCAGTCGACACGCGTGGGTTATTTTATCCAACCGTTGTTGCTGGGCTTTGGCTTTTTCTGGCTTACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAg             <  1:4270417/150‑1 (MQ=255)
                                                                                                                                           ggtacccgcagTCGACCCGGGTGGGTTATTTTAACCAACCGTTTTTGGTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCCGGGGCCAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCa           <  1:453453/143‑1 (MQ=255)
                                                                                                                                                 agcagTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCTAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTg     <  2:4454635/150‑1 (MQ=255)
                                                                                                                                                 agcagTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTg     >  1:3757370/1‑150 (MQ=255)
                                                                                                                                                 agcagTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTg     >  2:890893/1‑150 (MQ=255)
                                                                                                                                                    agTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGgcg  >  1:496479/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCG  >  CP009273/2303459‑2303756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A