Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,303,607 A→C 9.3% L93R (CTG→CGG)  ada ← fused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,303,6070AC 9.3% 722.7 / 15.7 316L93R (CTG→CGG) adafused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (121/152);  new base C (36/4);  total (157/159)
Fisher's exact test for biased strand distribution p-value = 3.00e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 336 total aligned reads displayed.

ATTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCG  >  CP009273/2303458‑2303756
                                                                                                                                                     |                                                                                                                                                      
aTTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCcagca                                                                                                                                                        <  2:134808/150‑1 (MQ=255)
   cgcgCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTc                                                                                                                                                     >  2:1137666/1‑150 (MQ=255)
   cgcgCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGGTCCAGCAGTc                                                                                                                                                     >  2:1907722/1‑150 (MQ=255)
      gcAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGac                                                                                                                                                  <  1:3258829/150‑1 (MQ=255)
      gcAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGac                                                                                                                                                  <  1:2407618/150‑1 (MQ=255)
       cAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGaca                                                                                                                                                 >  2:3486593/1‑150 (MQ=255)
        aaaCGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGacac                                                                                                                                                <  2:3191165/150‑1 (MQ=255)
         aaCGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACAcg                                                                                                                                               >  1:2695973/1‑150 (MQ=255)
           cGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCGTCCAGCGTTACAGGCGTGTCCTGTTCCAGCCGTCGACAcgcg                                                                                                                                             >  1:2788248/1‑150 (MQ=255)
           cGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGAGGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACAcgcg                                                                                                                                             <  2:1680191/150‑1 (MQ=255)
              cGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTgg                                                                                                                                          <  2:2491589/150‑1 (MQ=255)
               gAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCCGTCGACACGCGTggg                                                                                                                                         >  1:3596907/1‑150 (MQ=255)
                aGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGt                                                                                                                                        >  1:695991/1‑150 (MQ=255)
                 gcgcgcCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTg                                                                                                                                       <  1:1166579/150‑1 (MQ=255)
                 gcgcgcCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTg                                                                                                                                       <  2:3630802/150‑1 (MQ=255)
                    cgcCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGAtt                                                                                                                                    <  2:3256362/150‑1 (MQ=255)
                    cgcCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGAtt                                                                                                                                    <  1:1143209/150‑1 (MQ=255)
                       cAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTCACAGGCGTTTCCTGTTCCAGCAGTCGCCACGCGTGGGTGATTTTa                                                                                                                                 >  2:3717091/1‑150 (MQ=255)
                           ccTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCa                                                                                                                             <  1:1493593/150‑1 (MQ=255)
                            cTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCAGTTCCATCAGTCGACACGCGTGGGTGATTTTATACaa                                                                                                                            >  1:924570/1‑150 (MQ=255)
                            cTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCAGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCaa                                                                                                                            >  1:1415776/1‑150 (MQ=255)
                              gTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAAcc                                                                                                                          <  1:1355497/150‑1 (MQ=255)
                                 gCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGAt                                                                                                                       <  1:1112257/150‑1 (MQ=255)
                                  ccAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATg                                                                                                                      <  1:761123/150‑1 (MQ=255)
                                    aGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGtt                                                                                                                    <  1:2359193/150‑1 (MQ=255)
                                    aGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGtt                                                                                                                    <  2:3801025/150‑1 (MQ=255)
                                          tAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑TCTggg                                                                                                             >  1:1280971/1‑150 (MQ=255)
                                             gCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTCAGGCTTCCAGCGTTACAGGCGTGTCCGGTTCCAGCAGTCGACACGCGTGGGGGCTTTTATCCAACCGAGGGT‑GCTGGGcct                                                                                                          >  2:2211220/1‑148 (MQ=255)
                                             gCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCCGTCGACACGCGGGGGTGATTTTATCCAACCGAGGTT‑GCTGGGCAt                                                                                                          >  2:4192288/1‑150 (MQ=255)
                                             gCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATACAACCGATGTT‑GCTGGGcct                                                                                                          >  1:1727239/1‑148 (MQ=255)
                                              cGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATTGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCAGTTACAGCAGTCGACACCCCGGGGTGATTTTATACAAACGAAGTT‑TCTggggctt                                                                                                         >  1:4116566/1‑146 (MQ=255)
                                              cGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCAtt                                                                                                         <  1:2946190/150‑1 (MQ=255)
                                               gTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTg                                                                                                        <  1:3519290/150‑1 (MQ=255)
                                                tCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTgg                                                                                                       <  2:1123681/150‑1 (MQ=255)
                                                 cATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCAACGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTGTCCTGTTCCAGCAGTCGACACCCGTTGGTGATTTTATACAACCCATTTT‑TCTGGGCATTGGc                                                                                                      >  1:49326/1‑150 (MQ=255)
                                                  aTTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCt                                                                                                     >  2:2461177/1‑150 (MQ=255)
                                                   ttCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTT‑CAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCttt                                                                                                   <  2:3997221/150‑1 (MQ=255)
                                                     ccGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCtttt                                                                                                  <  2:910728/150‑1 (MQ=255)
                                                      cGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGTTTTTATCCAACCGATGTT‑GCTGGGCCTTGGCttttt                                                                                                 >  2:2896425/1‑150 (MQ=255)
                                                      cGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCttttt                                                                                                 <  1:1541434/150‑1 (MQ=255)
                                                        gTAGTCGCTTTAAACAACCGATGTAGATGAAAAGGACTCATCGCCACCAGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTGCAGGTACACCCGTCGACACCCGTGGGTGATTTTATACAACCGATTTT‑TGTGGGCAATGGCtttttaa                                                                                               >  1:1069257/1‑148 (MQ=255)
                                                            tCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCCGCCGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCt                                                                                           >  2:3391345/1‑150 (MQ=255)
                                                                 ttAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACCCGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACaa                                                                                      >  2:4048077/1‑150 (MQ=255)
                                                                  tAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCCGTCGACACGCGTGGGTGATTTTATCCAAACGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAAc                                                                                     >  1:4171766/1‑150 (MQ=255)
                                                                   aaaCAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACg                                                                                    >  2:3165774/1‑150 (MQ=255)
                                                                   aaaCAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACg                                                                                    <  1:999942/150‑1 (MQ=255)
                                                                   aaaCAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACg                                                                                    <  2:2357711/150‑1 (MQ=255)
                                                                      caacCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGttt                                                                                 <  2:3771160/150‑1 (MQ=255)
                                                                      caacCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGttt                                                                                 <  1:4260457/150‑1 (MQ=255)
                                                                      caacCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGttt                                                                                 <  2:848847/150‑1 (MQ=255)
                                                                           gATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAgg                                                                            <  2:2096928/150‑1 (MQ=255)
                                                                            aTGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAggg                                                                           <  1:161658/150‑1 (MQ=255)
                                                                             tGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAgggg                                                                          <  1:771460/150‑1 (MQ=255)
                                                                             tGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAgggg                                                                          <  2:3030343/150‑1 (MQ=255)
                                                                              gTAGATGAAATGGACTCATTGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCCGGGGc                                                                         >  2:1893259/1‑150 (MQ=255)
                                                                              gTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCCGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGc                                                                         >  1:2337261/1‑150 (MQ=255)
                                                                              gTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCCTTGGCGTTTTCGGGCTGACAACGTTTGCAGGGGc                                                                         >  2:2991380/1‑150 (MQ=255)
                                                                               tAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCg                                                                        <  1:1955200/150‑1 (MQ=255)
                                                                                aGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGa                                                                       >  2:1308194/1‑150 (MQ=255)
                                                                                aGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGa                                                                       >  2:1446307/1‑150 (MQ=255)
                                                                                 gATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCCGTCGCCACGCGGGGGTGATTTTATCCAACCGCTGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACCACGTTTGCAGGGGCgca                                                                      >  2:1094760/1‑148 (MQ=255)
                                                                                 gATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCCGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTTCAGGGGCGaa                                                                      >  2:465698/1‑150 (MQ=255)
                                                                                 gATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGaa                                                                      <  2:2070260/150‑1 (MQ=255)
                                                                                   tGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAg                                                                    <  1:2989822/150‑1 (MQ=255)
                                                                                     aaaTGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACACGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGcc                                                                  >  1:598485/1‑150 (MQ=255)
                                                                                       aTGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTGTCCGGTTCCAGCCGGCGCCCCGCGGGGGGGATTTTATCCAACCGATGTT‑GCGGGGCATTGGCTTTTTCTGGCGGACAACGTTTGCAGGGGCGAAAGCCAg                                                                >  1:3068090/1‑150 (MQ=255)
                                                                                        tGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCCGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAgc                                                               >  1:2174697/1‑150 (MQ=255)
                                                                                         ggACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAgcg                                                              <  1:1005054/150‑1 (MQ=255)
                                                                                          gACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAgcgg                                                             >  2:710405/1‑150 (MQ=255)
                                                                                          gACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGAGGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAgcgg                                                             >  2:2880446/1‑150 (MQ=255)
                                                                                           aCTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCCGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCCgcggc                                                            >  1:3893746/1‑150 (MQ=255)
                                                                                              cATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCAGTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAg                                                         >  2:3525316/1‑150 (MQ=255)
                                                                                                  gCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGCTGTT‑GCTGGGCCTTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGcc                                                     >  1:559280/1‑150 (MQ=255)
                                                                                                    cagcTGGGCGGATAAGGCTTCCAGGGTTACTGGCGTTTCTTTTTCCTGCAGTCGACCCGGGTGGGTTTTTTTATCCAACCGTTGTT‑GCTGGGCTTTGGCTTTTTGTGGCTTAAAACTTTTGCTGGGGCGAAAGCCAGCGGCGAGTGCCTc                                                   <  1:3772524/147‑1 (MQ=255)
                                                                                                    cACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATACAAACGCTGTT‑TGTGGGGATTTGCTTTTTATGGCTGACAACGTTTTCATGGGCGAAAGCCAGCGGCGAGTGCCTc                                                   >  1:3402764/1‑150 (MQ=255)
                                                                                                      ccTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTTCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGc                                                 >  2:3680221/1‑150 (MQ=255)
                                                                                                       cTGGTCGGATAAGGCTACCAGCGTTACCGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCt                                                <  2:3323111/150‑1 (MQ=255)
                                                                                                          gTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGc                                             <  2:4325408/150‑1 (MQ=255)
                                                                                                                tAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCCGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCCTTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCCTTTGc                                       >  2:771994/1‑150 (MQ=255)
                                                                                                                tAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACCCGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACTTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGc                                       <  2:1711689/150‑1 (MQ=255)
                                                                                                                   ggCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTa                                    >  2:2394865/1‑150 (MQ=255)
                                                                                                                    gCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAg                                   >  2:588549/1‑150 (MQ=255)
                                                                                                                       tCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGATTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAg                                <  2:2603824/150‑1 (MQ=255)
                                                                                                                        ccAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAgg                               <  2:2340745/150‑1 (MQ=255)
                                                                                                                        ccAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCGGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAgg                               <  2:691012/150‑1 (MQ=255)
                                                                                                                         cAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCTTTGGCTTTTTCTGGCTGACACCGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGga                              <  2:3892743/150‑1 (MQ=255)
                                                                                                                          aGCGTTACCGGCGTTTCCTGTTCCAGCCGCCGACCCGCGTGGGTTTTTTAAACCAACCG‑TGTTGGCTGGGATTGGGGTTTTTCTGGCTGACAAGGTTTGCTGGGGCCAACCCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGgag                             <  1:372882/150‑1 (MQ=255)
                                                                                                                           gCGTTACCGGCGGTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCGGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGgaga                            <  1:3883667/150‑1 (MQ=255)
                                                                                                                                 cAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGtttt                      >  1:3731469/1‑150 (MQ=255)
                                                                                                                                     cGTTTCCTGTTCCAGCAGTCGACAGGCGTGGTTTTTTTTATCCAACCGAGGTT‑GGTGGGAATTGGATTTTTCTGGCTGACAACGTTTGCTGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCg                  <  1:4048077/150‑1 (MQ=255)
                                                                                                                                        ttCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCaa               >  1:1012745/1‑150 (MQ=255)
                                                                                                                                        ttCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCaa               >  1:4356512/1‑150 (MQ=255)
                                                                                                                                        ttCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCaa               <  2:2788248/150‑1 (MQ=255)
                                                                                                                                        ttCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCaa               >  2:1253866/1‑150 (MQ=255)
                                                                                                                                            tGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCa           >  2:3457737/1‑150 (MQ=255)
                                                                                                                                                 cagcagTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCt      <  1:2536285/150‑1 (MQ=255)
                                                                                                                                                    cagTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGgc   >  1:2471238/1‑150 (MQ=255)
                                                                                                                                                    cagTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGgc   >  1:2612695/1‑150 (MQ=255)
                                                                                                                                                     agTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGgcg  <  1:2099162/150‑1 (MQ=255)
                                                                                                                                                     agTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGgcg  >  1:2040307/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
ATTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAATGGACTCATCGCCACCTGGTCGGCTAAGGCTTCCAGCGTTACAGGCGTTTCCTGTTCCAGCAGTCGACACGCGTGGGTGATTTTATCCAACCGATGTT‑GCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCG  >  CP009273/2303458‑2303756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A