Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,375,260 G→T 83.7% D387Y (GAT→TAT)  degQ → serine endoprotease, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,375,2600GT83.7% 624.3 / 129.2 262D387Y (GAT→TAT) degQserine endoprotease, periplasmic
Reads supporting (aligned to +/- strand):  ref base G (20/23);  new base T (103/116);  total (123/139)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

Only 100 of 262 total aligned reads displayed.

GGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAA  >  CP009273/3375113‑3375406
                                                                                                                                                   |                                                                                                                                                  
ggCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGAt                                                                                                                                                  <  2:1994877/150‑1 (MQ=255)
      aaaGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCCGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATggcggc                                                                                                                                            >  1:124592/1‑150 (MQ=255)
      aaaGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATggcggc                                                                                                                                            <  1:506784/150‑1 (MQ=255)
        aGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCaa                                                                                                                                          >  2:4207904/1‑150 (MQ=255)
        aGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCaa                                                                                                                                          >  2:2021060/1‑150 (MQ=255)
        aGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCaa                                                                                                                                          <  1:1640226/150‑1 (MQ=255)
        aGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCaa                                                                                                                                          >  1:4471477/1‑150 (MQ=255)
         gTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCaaa                                                                                                                                         >  2:966972/1‑150 (MQ=255)
         gTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCaaa                                                                                                                                         >  2:3837954/1‑150 (MQ=255)
          tGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAg                                                                                                                                        >  2:3399135/1‑150 (MQ=255)
          tGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAg                                                                                                                                        <  2:3306250/150‑1 (MQ=255)
          tGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAg                                                                                                                                        <  1:2525104/150‑1 (MQ=255)
          tGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAg                                                                                                                                        <  1:2332410/150‑1 (MQ=255)
          tGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAg                                                                                                                                        <  1:3031581/150‑1 (MQ=255)
           gAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAgg                                                                                                                                       <  2:790032/150‑1 (MQ=255)
             aGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTa                                                                                                                                     <  2:2448080/150‑1 (MQ=255)
               cTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTAtt                                                                                                                                   <  2:3945529/150‑1 (MQ=255)
               cTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTAtt                                                                                                                                   <  1:3580698/150‑1 (MQ=255)
                 tGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTaa                                                                                                                                 <  1:600497/150‑1 (MQ=255)
                 tGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTaa                                                                                                                                 <  1:1330725/150‑1 (MQ=255)
                    ccTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAAt                                                                                                                              >  1:1449991/1‑150 (MQ=255)
                     ctgctgCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAACTc                                                                                                                             >  2:2519263/1‑150 (MQ=255)
                     ctgctgCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATc                                                                                                                             <  1:384442/150‑1 (MQ=255)
                      tgctgcGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCg                                                                                                                            <  2:2882147/150‑1 (MQ=255)
                      tgctgcGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCg                                                                                                                            <  2:3862977/150‑1 (MQ=255)
                       gctgcGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTAATAAAATCGa                                                                                                                           <  1:4103418/150‑1 (MQ=255)
                         tgcGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATg                                                                                                                         >  1:1687643/1‑150 (MQ=255)
                         tgcGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATg                                                                                                                         >  2:1662403/1‑150 (MQ=255)
                         tgcGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATg                                                                                                                         <  2:3012915/150‑1 (MQ=255)
                            gTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAg                                                                                                                      <  1:4087450/150‑1 (MQ=255)
                             tAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGt                                                                                                                     >  1:843858/1‑150 (MQ=255)
                               aCGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTg                                                                                                                   <  1:3832277/150‑1 (MQ=255)
                                cGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGt                                                                                                                  <  2:865100/150‑1 (MQ=255)
                                 ggCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTc                                                                                                                 <  2:1443193/150‑1 (MQ=255)
                                 ggCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTc                                                                                                                 >  2:3839356/1‑150 (MQ=255)
                                  gCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCa                                                                                                                <  1:719277/150‑1 (MQ=255)
                                        cACTGTAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGaa                                                                                                          <  2:3597511/150‑1 (MQ=255)
                                            ggAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGccc                                                                                                      <  1:3411598/150‑1 (MQ=255)
                                                gTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAgct                                                                                                  <  2:1524758/150‑1 (MQ=255)
                                                    aaGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTc                                                                                              <  1:1852513/150‑1 (MQ=255)
                                                     aGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCa                                                                                             <  1:2626131/150‑1 (MQ=255)
                                                     aGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCa                                                                                             <  1:361967/150‑1 (MQ=255)
                                                     aGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCa                                                                                             >  2:3657173/1‑150 (MQ=255)
                                                     aGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCGGGAAGGTGCAACGTTGAGCGATGGTCAGCTCAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCa                                                                                             >  2:3059950/1‑150 (MQ=255)
                                                      gTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAg                                                                                            <  2:1359713/150‑1 (MQ=255)
                                                      gTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAg                                                                                            <  2:2945929/150‑1 (MQ=255)
                                                       tGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCGGGAAGGTGCAACGTTGAGCGATGGTCAGCGCAAATATGGCGGCAAAGGGATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAgg                                                                                           >  1:106186/1‑150 (MQ=255)
                                                          cGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAggctg                                                                                        <  1:3328537/150‑1 (MQ=255)
                                                           gCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAggctgg                                                                                       >  2:2861535/1‑150 (MQ=255)
                                                           gCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAggctgg                                                                                       <  1:3429388/150‑1 (MQ=255)
                                                            cTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCCGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAggctggc                                                                                      >  1:801278/1‑150 (MQ=255)
                                                               gATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTg                                                                                   >  2:3634465/1‑150 (MQ=255)
                                                                aTACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGc                                                                                  >  1:3568328/1‑150 (MQ=255)
                                                                aTACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGc                                                                                  >  2:3208131/1‑150 (MQ=255)
                                                                      gCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCaaaaac                                                                            >  1:3095274/1‑149 (MQ=255)
                                                                        aCCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGAc                                                                          >  2:2442489/1‑150 (MQ=255)
                                                                        aCCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGAc                                                                          <  2:318002/150‑1 (MQ=255)
                                                                        aCCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGAc                                                                          <  1:3546130/150‑1 (MQ=255)
                                                                           tctTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGTAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGAt                                                                       >  1:900511/1‑150 (MQ=255)
                                                                           tctTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGAt                                                                       <  1:2924838/150‑1 (MQ=255)
                                                                               cgtcgGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGa                                                                   >  2:4006366/1‑150 (MQ=255)
                                                                               cgtcgGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGGTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGa                                                                   <  2:542687/150‑1 (MQ=255)
                                                                                  cgGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTCTTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCa                                                                >  1:1831236/1‑150 (MQ=255)
                                                                                     ccAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTg                                                             <  2:4288508/150‑1 (MQ=255)
                                                                                       aGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGc                                                           >  2:3324259/1‑150 (MQ=255)
                                                                                        gcgcTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCg                                                          <  2:4139343/150‑1 (MQ=255)
                                                                                           cTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCa                                                       <  1:242909/150‑1 (MQ=255)
                                                                                            tGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCaa                                                      >  2:1858193/1‑150 (MQ=255)
                                                                                             gAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAAc                                                     >  1:3466165/1‑150 (MQ=255)
                                                                                             gAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAAc                                                     >  2:4185/1‑150 (MQ=255)
                                                                                              aaaTGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACTATGTGATCATTGGCGTCAAcc                                                    >  2:1045868/1‑150 (MQ=255)
                                                                                              aaaTGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAAcc                                                    <  1:3395982/150‑1 (MQ=255)
                                                                                              aaaTGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAAcc                                                    <  1:4357556/150‑1 (MQ=255)
                                                                                              aaaTGATCACGACTGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAACATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAAcc                                                    <  2:2549808/150‑1 (MQ=255)
                                                                                               aaTGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACcg                                                   <  1:1622565/150‑1 (MQ=255)
                                                                                               aaTGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACcg                                                   >  2:1910576/1‑150 (MQ=255)
                                                                                               aaTGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACcg                                                   <  2:638050/150‑1 (MQ=255)
                                                                                                   aTCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGAt                                               <  1:2000853/150‑1 (MQ=255)
                                                                                                    tCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATc                                              >  2:3981862/1‑150 (MQ=255)
                                                                                                      aCGCCAGCGCTGGAAGGTGCAACGTTGAGCGATTGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGATGTCAAAGTAAGACCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGAGATCATAGGCGTCAACCGCGaaccg                                            >  2:1287775/1‑146 (MQ=255)
                                                                                                      aCGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCgg                                            >  2:3697677/1‑150 (MQ=255)
                                                                                                        gCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGt                                          >  2:360378/1‑150 (MQ=255)
                                                                                                        gCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGt                                          <  2:4348856/150‑1 (MQ=255)
                                                                                                          cAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGa                                        >  1:2279336/1‑150 (MQ=255)
                                                                                                             cgcTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACt                                     <  1:3256954/150‑1 (MQ=255)
                                                                                                              gcTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTc                                    >  2:1725203/1‑150 (MQ=255)
                                                                                                                 ggAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGAt                                 >  1:712665/1‑150 (MQ=255)
                                                                                                                   aaGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTg                               <  2:756348/150‑1 (MQ=255)
                                                                                                                   aaGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTg                               <  1:1817959/150‑1 (MQ=255)
                                                                                                                        gCACCGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGaa                          <  1:2372746/150‑1 (MQ=255)
                                                                                                                         cAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGaaa                         >  2:2265857/1‑150 (MQ=255)
                                                                                                                         cAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGaaa                         >  2:830469/1‑150 (MQ=255)
                                                                                                                                gAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTaa                  >  1:2092624/1‑150 (MQ=255)
                                                                                                                                 aGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTaaa                 >  2:2319323/1‑150 (MQ=255)
                                                                                                                                  gCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAg                <  1:2232085/150‑1 (MQ=255)
                                                                                                                                     aTGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGc             >  2:3203106/1‑150 (MQ=255)
                                                                                                                                          cAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTggcg        <  2:150210/150‑1 (MQ=255)
                                                                                                                                             cTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCa     >  1:3875860/1‑150 (MQ=255)
                                                                                                                                             cTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCa     >  2:4513507/1‑150 (MQ=255)
                                                                                                                                              tAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCaa    >  1:2779082/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAA  >  CP009273/3375113‑3375406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAAC  >  CP009273/3375112‑3375407
                                                                                                                                                    |                                                                                                                                                   
GGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATA                                                                                                                                                    <  SRR19973080.4548750/150‑1 (MQ=60)
  GCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATG                                                                                                                                                  <  SRR19973080.2111869/150‑1 (MQ=60)
   CACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGG                                                                                                                                                 <  SRR19973080.842555/150‑1 (MQ=60)
    ACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGC                                                                                                                                                <  SRR19973080.1598191/150‑1 (MQ=60)
      GAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGG                                                                                                                                              <  SRR19973080.2492329/150‑1 (MQ=60)
       AAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCCGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGC                                                                                                                                             >  SRR19973080.125653/1‑150 (MQ=60)
       AAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGACGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGC                                                                                                                                             >  SRR19973080.510835/1‑150 (MQ=60)
       AAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGC                                                                                                                                             <  SRR19973080.510021/150‑1 (MQ=60)
       AAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGC                                                                                                                                             >  SRR19973080.2377071/1‑150 (MQ=60)
         AGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAA                                                                                                                                           >  SRR19973080.4495688/1‑150 (MQ=60)
         AGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAA                                                                                                                                           <  SRR19973080.1649802/150‑1 (MQ=60)
         AGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAA                                                                                                                                           <  SRR19973080.1649826/150‑1 (MQ=60)
          GTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAA                                                                                                                                          >  SRR19973080.4153745/1‑150 (MQ=60)
           TGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAG                                                                                                                                         <  SRR19973080.2538993/150‑1 (MQ=60)
           TGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAG                                                                                                                                         <  SRR19973080.3047900/150‑1 (MQ=60)
           TGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAG                                                                                                                                         <  SRR19973080.2345386/150‑1 (MQ=60)
              AGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTA                                                                                                                                      <  SRR19973080.3477167/150‑1 (MQ=60)
                CTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATT                                                                                                                                    <  SRR19973080.626527/150‑1 (MQ=60)
                CTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATT                                                                                                                                    <  SRR19973080.3600499/150‑1 (MQ=60)
                CTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATT                                                                                                                                    <  SRR19973080.2823149/150‑1 (MQ=60)
                CTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATT                                                                                                                                    <  SRR19973080.3464788/150‑1 (MQ=60)
                  TGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAA                                                                                                                                  <  SRR19973080.604245/150‑1 (MQ=60)
                  TGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAA                                                                                                                                  <  SRR19973080.604211/150‑1 (MQ=60)
                  TGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAA                                                                                                                                  <  SRR19973080.1338749/150‑1 (MQ=60)
                     CCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAAT                                                                                                                               >  SRR19973080.1458411/1‑150 (MQ=60)
                      CTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATC                                                                                                                              <  SRR19973080.4143056/150‑1 (MQ=60)
                      CTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATC                                                                                                                              <  SRR19973080.386627/150‑1 (MQ=60)
                        GCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTAATAAAATCGA                                                                                                                            <  SRR19973080.4125718/150‑1 (MQ=60)
                         CTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGAT                                                                                                                           >  SRR19973080.2725644/1‑150 (MQ=60)
                          TGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATG                                                                                                                          >  SRR19973080.1418344/1‑150 (MQ=60)
                          TGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATG                                                                                                                          >  SRR19973080.1697475/1‑150 (MQ=60)
                          TGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATG                                                                                                                          >  SRR19973080.3713807/1‑150 (MQ=60)
                             GTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAG                                                                                                                       <  SRR19973080.4109712/150‑1 (MQ=60)
                              TAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGT                                                                                                                      >  SRR19973080.848947/1‑150 (MQ=60)
                               AACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTT                                                                                                                     >  SRR19973080.219045/1‑150 (MQ=60)
                                ACGGCACACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTG                                                                                                                    <  SRR19973080.3854754/150‑1 (MQ=60)
                                ACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTG                                                                                                                    <  SRR19973080.3853319/150‑1 (MQ=60)
                                   GCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCA                                                                                                                 <  SRR19973080.723421/150‑1 (MQ=60)
                                         CACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAA                                                                                                           <  SRR19973080.759202/150‑1 (MQ=60)
                                         CACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAA                                                                                                           <  SRR19973080.759162/150‑1 (MQ=60)
                                         CACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAA                                                                                                           <  SRR19973080.1690352/150‑1 (MQ=60)
                                             GGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCC                                                                                                       <  SRR19973080.3430098/150‑1 (MQ=60)
                                             GGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCC                                                                                                       <  SRR19973080.3430090/150‑1 (MQ=60)
                                                  TAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTG                                                                                                  >  SRR19973080.1823551/1‑150 (MQ=60)
                                                  TAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCGGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTG                                                                                                  >  SRR19973080.3347821/1‑150 (MQ=60)
                                                     AAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTC                                                                                               <  SRR19973080.1864591/150‑1 (MQ=60)
                                                     AAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTC                                                                                               <  SRR19973080.1862904/150‑1 (MQ=60)
                                                      AGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCA                                                                                              <  SRR19973080.364028/150‑1 (MQ=60)
                                                      AGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCA                                                                                              <  SRR19973080.364049/150‑1 (MQ=60)
                                                      AGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCA                                                                                              <  SRR19973080.2640351/150‑1 (MQ=60)
                                                       GTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAG                                                                                             <  SRR19973080.48635/150‑1 (MQ=60)
                                                        TGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCGGGAAGGTGCAACGTTGAGCGATGGTCAGCGCAAATATGGCGGCAAAGGGATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGG                                                                                            >  SRR19973080.107133/1‑150 (MQ=60)
                                                           CGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTG                                                                                         <  SRR19973080.3346682/150‑1 (MQ=60)
                                                           CGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTG                                                                                         <  SRR19973080.3238404/150‑1 (MQ=60)
                                                            GCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGG                                                                                        <  SRR19973080.3448057/150‑1 (MQ=60)
                                                            GCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGG                                                                                        >  SRR19973080.2277086/1‑150 (MQ=60)
                                                             CTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCCGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGC                                                                                       >  SRR19973080.805970/1‑150 (MQ=60)
                                                             CTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCGGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGTAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGC                                                                                       >  SRR19973080.1154932/1‑150 (MQ=60)
                                                               CGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTT                                                                                     <  SRR19973080.3417184/150‑1 (MQ=60)
                                                               CGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTT                                                                                     <  SRR19973080.3417504/150‑1 (MQ=60)
                                                                GATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTG                                                                                    <  SRR19973080.3566889/150‑1 (MQ=60)
                                                                 ATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGC                                                                                   >  SRR19973080.1292239/1‑150 (MQ=60)
                                                                 ATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGC                                                                                   >  SRR19973080.3588073/1‑150 (MQ=60)
                                                                  TACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCA                                                                                  <  SRR19973080.4483746/150‑1 (MQ=60)
                                                                  TACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCA                                                                                  <  SRR19973080.4483588/150‑1 (MQ=60)
                                                                   ACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAA                                                                                 >  SRR19973080.4011785/1‑150 (MQ=60)
                                                                   ACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAA                                                                                 >  SRR19973080.4011681/1‑150 (MQ=60)
                                                                       GCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAC                                                                             >  SRR19973080.3112235/1‑150 (MQ=60)
                                                                       GCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATAGATGAAGTTTTCAAAGGAAGCCCACCTGCTCAGGCTGGCTTGCAAAAAG                                                                             >  SRR19973080.3110566/1‑150 (MQ=60)
                                                                        CACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGA                                                                            >  SRR19973080.2436097/1‑150 (MQ=60)
                                                                         ACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGAC                                                                           <  SRR19973080.718583/150‑1 (MQ=60)
                                                                         ACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGAC                                                                           <  SRR19973080.3565746/150‑1 (MQ=60)
                                                                            TCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATTGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAACCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGAT                                                                        >  SRR19973080.3572140/1‑150 (MQ=60)
                                                                            TCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGTAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGAT                                                                        >  SRR19973080.906023/1‑150 (MQ=60)
                                                                            TCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGAT                                                                        <  SRR19973080.892767/150‑1 (MQ=60)
                                                                            TCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGAT                                                                        <  SRR19973080.2940756/150‑1 (MQ=60)
                                                                              TTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGT                                                                      >  SRR19973080.1806464/1‑150 (MQ=60)
                                                                               TCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTG                                                                     <  SRR19973080.3859035/150‑1 (MQ=60)
                                                                                  TCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATC                                                                  >  SRR19973080.3649773/1‑150 (MQ=60)
                                                                                  TCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATC                                                                  >  SRR19973080.4125370/1‑150 (MQ=60)
                                                                                   CGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTCTTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCA                                                                 >  SRR19973080.1841566/1‑150 (MQ=60)
                                                                                   CGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCA                                                                 >  SRR19973080.1840511/1‑150 (MQ=60)
                                                                                   CGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCA                                                                 >  SRR19973080.3132664/1‑150 (MQ=60)
                                                                                   CGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCA                                                                 <  SRR19973080.534301/150‑1 (MQ=60)
                                                                                    GGCCAGCGCTGAAATGATCACGCCAGCGCTGGTATGTTCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCAT                                                                <  SRR19973080.865603/150‑1 (MQ=60)
                                                                                     GCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATT                                                               <  SRR19973080.3896481/150‑1 (MQ=60)
                                                                                          CGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGT                                                          >  SRR19973080.2569579/1‑150 (MQ=60)
                                                                                            CTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCA                                                        <  SRR19973080.244590/150‑1 (MQ=60)
                                                                                              GAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAAC                                                      >  SRR19973080.3485174/1‑150 (MQ=60)
                                                                                              GAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAAC                                                      >  SRR19973080.3485175/1‑150 (MQ=60)
                                                                                               AAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACC                                                     <  SRR19973080.3414332/150‑1 (MQ=60)
                                                                                               AAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACC                                                     >  SRR19973080.127364/1‑150 (MQ=60)
                                                                                               AAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACC                                                     <  SRR19973080.4381335/150‑1 (MQ=60)
                                                                                                AATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCG                                                    <  SRR19973080.1632117/150‑1 (MQ=60)
                                                                                                AATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCG                                                    <  SRR19973080.1632070/150‑1 (MQ=60)
                                                                                                 ATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGC                                                   >  SRR19973080.2558481/1‑150 (MQ=60)
                                                                                                 ATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGC                                                   >  SRR19973080.3590180/1‑150 (MQ=60)
                                                                                                 ATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGC                                                   >  SRR19973080.3590178/1‑150 (MQ=60)
                                                                                                    ATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGAT                                                <  SRR19973080.2920356/150‑1 (MQ=60)
                                                                                                    ATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGAT                                                <  SRR19973080.2012227/150‑1 (MQ=60)
                                                                                                          CCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTG                                          >  SRR19973080.2451092/1‑150 (MQ=60)
                                                                                                           CAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAATGACGAGGTGATCATTGGCGTCAACCGCGATCGGGTGG                                         >  SRR19973080.2291843/1‑150 (MQ=60)
                                                                                                             GCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAAC                                       <  SRR19973080.3034657/150‑1 (MQ=60)
                                                                                                              CGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACT                                      <  SRR19973080.3274871/150‑1 (MQ=60)
                                                                                                              CGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACT                                      <  SRR19973080.3315657/150‑1 (MQ=60)
                                                                                                                  GGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGAT                                  >  SRR19973080.2989099/1‑150 (MQ=60)
                                                                                                                  GGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGAT                                  >  SRR19973080.716792/1‑150 (MQ=60)
                                                                                                                    AAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTG                                <  SRR19973080.1828246/150‑1 (MQ=60)
                                                                                                                         GCACCGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAA                           <  SRR19973080.2385940/150‑1 (MQ=60)
                                                                                                                         GCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAA                           <  SRR19973080.2385911/150‑1 (MQ=60)
                                                                                                                          CAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAA                          >  SRR19973080.187089/1‑150 (MQ=60)
                                                                                                                          CAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAA                          >  SRR19973080.2300672/1‑150 (MQ=60)
                                                                                                                           AACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAAT                         <  SRR19973080.976503/150‑1 (MQ=60)
                                                                                                                              GTTGAGCGATGGTCAGCTAAAATATGGCGGCAAGGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCG                      <  SRR19973080.1856637/150‑1 (MQ=60)
                                                                                                                              GTTGAGCGATGGTCAGCTAAAATATGGCGGCAAGGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCG                      <  SRR19973080.1856692/150‑1 (MQ=60)
                                                                                                                                 GAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAA                   >  SRR19973080.2104441/1‑150 (MQ=60)
                                                                                                                                   GCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAG                 <  SRR19973080.2244371/150‑1 (MQ=60)
                                                                                                                                         GTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGG           <  SRR19973080.2095675/150‑1 (MQ=60)
                                                                                                                                              CTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCA      >  SRR19973080.1602554/1‑150 (MQ=60)
                                                                                                                                              CTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCA      <  SRR19973080.2279836/150‑1 (MQ=60)
                                                                                                                                              CTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCA      >  SRR19973080.3897213/1‑150 (MQ=60)
                                                                                                                                              CTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCA      >  SRR19973080.3392789/1‑150 (MQ=60)
                                                                                                                                               TAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAA     <  SRR19973080.958041/150‑1 (MQ=60)
                                                                                                                                               TAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAA     >  SRR19973080.2794415/1‑150 (MQ=60)
                                                                                                                                                  AATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAAC  <  SRR19973080.466492/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
GGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAAC  >  CP009273/3375112‑3375407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑