Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,375,260 G→T 100% D387Y (GAT→TAT)  degQ → serine endoprotease, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,375,2600GT99.5% 689.7 / ‑4.1 209D387Y (GAT→TAT) degQserine endoprotease, periplasmic
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (92/116);  total (92/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 210 total aligned reads displayed.

CACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAA  >  CP009273/3375115‑3375404
                                                                                                                                                 |                                                                                                                                                
cACGAAAGTGAAGCTTGGCGTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAatat                                                                                                                                                <  1:171185/148‑1 (MQ=255)
  cGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATgg                                                                                                                                              >  1:3512790/1‑148 (MQ=255)
  cGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATgg                                                                                                                                              >  1:1665562/1‑148 (MQ=255)
   gAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATggc                                                                                                                                             <  1:618538/148‑1 (MQ=255)
       gTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCa                                                                                                                                         >  1:882398/1‑148 (MQ=255)
        tGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAACTGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCaa                                                                                                                                        >  1:3450840/1‑148 (MQ=255)
        tGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCaa                                                                                                                                        <  2:2438758/148‑1 (MQ=255)
         gAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCaaa                                                                                                                                       <  1:2904299/148‑1 (MQ=255)
          aaGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGATCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAg                                                                                                                                      <  2:951107/148‑1 (MQ=255)
          aaGCGTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAg                                                                                                                                      <  2:951296/148‑1 (MQ=255)
            gCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGt                                                                                                                                    <  1:2365847/148‑1 (MQ=255)
             cTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTa                                                                                                                                   <  2:2739862/148‑1 (MQ=255)
             cTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTa                                                                                                                                   <  2:1727216/148‑1 (MQ=255)
               tGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGTGATGGTCAGCTAAAATATGGCGGCAAAGGTAtt                                                                                                                                 >  2:3122977/1‑148 (MQ=255)
                  ccTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTaaa                                                                                                                              <  2:3136521/148‑1 (MQ=255)
                  ccTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTaaa                                                                                                                              <  2:2409374/148‑1 (MQ=255)
                    tgctgcGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAANTGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAAt                                                                                                                            <  1:1783458/148‑1 (MQ=255)
                    tgctgcGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAAt                                                                                                                            >  2:3279779/1‑148 (MQ=255)
                    tgctgcGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAAt                                                                                                                            <  2:2234329/148‑1 (MQ=255)
                     gctgcGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATc                                                                                                                           <  1:718989/148‑1 (MQ=255)
                       tgcGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGa                                                                                                                         <  1:1972384/148‑1 (MQ=255)
                            aaCGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAACTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAg                                                                                                                    <  1:1508245/148‑1 (MQ=255)
                               ggCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTg                                                                                                                 <  1:755665/148‑1 (MQ=255)
                                gCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGt                                                                                                                >  1:2166128/1‑148 (MQ=255)
                                gCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGt                                                                                                                >  1:2166273/1‑148 (MQ=255)
                                   aaCCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGTCGGCAAAGGTATTAAAATCGATGAAGTTGTCaa                                                                                                             <  2:3077473/148‑1 (MQ=255)
                                   aaCCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCaa                                                                                                             <  1:1120234/148‑1 (MQ=255)
                                   aaCCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCaa                                                                                                             <  1:1120110/148‑1 (MQ=255)
                                   aaCCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCaa                                                                                                             >  2:1354867/1‑148 (MQ=255)
                                    aCCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCCGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGATCGATGGTCAGCTAAAATATTGCGGCAAAGGTATTAAAATCGATGAAGTTGTCaaa                                                                                                            >  1:3400983/1‑148 (MQ=255)
                                       aCTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGa                                                                                                         >  1:350652/1‑148 (MQ=255)
                                       aCTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGa                                                                                                         >  2:1297487/1‑148 (MQ=255)
                                          ggAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGc                                                                                                      >  2:1593264/1‑148 (MQ=255)
                                            aaGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGccc                                                                                                    >  2:3014898/1‑148 (MQ=255)
                                                 gAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTCTTAAAATCGATGAAGTTGTCAAAGGACGCCCAgctg                                                                                               >  2:61900/1‑148 (MQ=255)
                                                 gAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAgctg                                                                                               <  2:580522/148‑1 (MQ=255)
                                                   aGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAgctgct                                                                                             >  2:2012347/1‑148 (MQ=255)
                                                       aCGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAgg                                                                                         >  1:2175050/1‑148 (MQ=255)
                                                       aCGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAgg                                                                                         <  2:146039/148‑1 (MQ=255)
                                                       aCGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAgg                                                                                         <  1:2844853/148‑1 (MQ=255)
                                                        cGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAggc                                                                                        <  1:1779045/148‑1 (MQ=255)
                                                          cTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAggctg                                                                                      >  2:1146035/1‑148 (MQ=255)
                                                            cGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAggctggc                                                                                    <  2:1172525/148‑1 (MQ=255)
                                                            cGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAggctggc                                                                                    <  2:739462/148‑1 (MQ=255)
                                                               tACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTg                                                                                 <  2:132058/148‑1 (MQ=255)
                                                                    gCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCaaaa                                                                            >  1:1092248/1‑148 (MQ=255)
                                                                     cACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCaaaaa                                                                           >  2:3373146/1‑148 (MQ=255)
                                                                      aCCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAg                                                                          >  1:2709511/1‑148 (MQ=255)
                                                                         tctTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACg                                                                       >  2:1736214/1‑148 (MQ=255)
                                                                         tctTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACg                                                                       <  1:753281/148‑1 (MQ=255)
                                                                         tcgtcgtcgGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACg                                                                       <  1:753942/145‑1 (MQ=255)
                                                                            tcgtcgGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGAtg                                                                    <  1:2060218/148‑1 (MQ=255)
                                                                            tcgtcgGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGAtg                                                                    <  1:289075/148‑1 (MQ=255)
                                                                             cgtcgGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGAtgt                                                                   >  1:584375/1‑148 (MQ=255)
                                                                                 ggCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATc                                                               <  2:2755306/148‑1 (MQ=255)
                                                                                 ggCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATc                                                               <  2:1873384/148‑1 (MQ=255)
                                                                                 ggCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATc                                                               <  1:790190/148‑1 (MQ=255)
                                                                                  gCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCa                                                              >  2:2167902/1‑148 (MQ=255)
                                                                                     aGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTg                                                           <  2:1222335/148‑1 (MQ=255)
                                                                                     aGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTg                                                           <  2:2280691/148‑1 (MQ=255)
                                                                                         cTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGt                                                       >  2:1068397/1‑148 (MQ=255)
                                                                                          tGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTc                                                      <  2:477391/148‑1 (MQ=255)
                                                                                           gAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCa                                                     >  1:2717017/1‑148 (MQ=255)
                                                                                           gAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCa                                                     <  1:832608/148‑1 (MQ=255)
                                                                                           gAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCa                                                     <  1:588267/148‑1 (MQ=255)
                                                                                           gAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCa                                                     <  1:3309818/148‑1 (MQ=255)
                                                                                                gATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACcgc                                                >  1:3054158/1‑148 (MQ=255)
                                                                                                 aTCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACcgcg                                               <  1:3279333/148‑1 (MQ=255)
                                                                                                  tCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGa                                              <  2:1947177/148‑1 (MQ=255)
                                                                                                  tCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGa                                              <  2:1429143/148‑1 (MQ=255)
                                                                                                     cGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGANGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCg                                           >  1:1781159/1‑148 (MQ=255)
                                                                                                     cGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGa                                              <  2:2464895/145‑1 (MQ=255)
                                                                                                     cGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGa                                              >  1:2464895/1‑145 (MQ=255)
                                                                                                     cGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCg                                           >  1:1077892/1‑148 (MQ=255)
                                                                                                     cGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCg                                           >  1:2826777/1‑148 (MQ=255)
                                                                                                          gcgcTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGa                                      <  2:2972728/148‑1 (MQ=255)
                                                                                                          gcgcTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGa                                      >  2:1293399/1‑148 (MQ=255)
                                                                                                               ggAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCg                                 <  2:2175050/148‑1 (MQ=255)
                                                                                                                gAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGa                                >  2:2814891/1‑148 (MQ=255)
                                                                                                                   ggTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTg                             <  1:1201320/148‑1 (MQ=255)
                                                                                                                   ggTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTg                             <  2:822298/148‑1 (MQ=255)
                                                                                                                   ggTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTg                             <  2:334020/148‑1 (MQ=255)
                                                                                                                     tGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACTATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCt                           >  1:32592/1‑148 (MQ=255)
                                                                                                                     tGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACTATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCt                           >  1:2629976/1‑148 (MQ=255)
                                                                                                                     tGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACTATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCt                           >  1:32767/1‑148 (MQ=255)
                                                                                                                        aaCGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGaa                        >  1:3391317/1‑148 (MQ=255)
                                                                                                                        aaCGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGaa                        <  2:676128/148‑1 (MQ=255)
                                                                                                                         aCGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGaaa                       <  2:947956/148‑1 (MQ=255)
                                                                                                                         aCGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGaaa                       >  1:1940750/1‑148 (MQ=255)
                                                                                                                         aCGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGaaa                       >  1:1602372/1‑148 (MQ=255)
                                                                                                                          cGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAAt                      >  2:3493917/1‑148 (MQ=255)
                                                                                                                            ttGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATT‑AAATCCATGTAGTTGTAAAAGGAAGCCCAGCTTCTCAGGCTGGCTTGCAAAAAGAAGATGTGATAATTGGCGTAAAACGCGATCGGGTGTAATCGATTGCTGAAATGCg                   >  2:1901623/1‑148 (MQ=255)
                                                                                                                             tGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCg                   >  2:263382/1‑148 (MQ=255)
                                                                                                                              gAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGt                  >  1:431123/1‑148 (MQ=255)
                                                                                                                                gCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTaa                <  2:2854005/148‑1 (MQ=255)
                                                                                                                                    tGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTg            <  1:1297487/148‑1 (MQ=255)
                                                                                                                                     ggTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGc           >  2:2523766/1‑148 (MQ=255)
                                                                                                                                      gTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCt          >  2:3203300/1‑148 (MQ=255)
                                                                                                                                           cTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTggcgg     <  1:1354867/148‑1 (MQ=255)
                                                                                                                                              aaaTATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCaa  <  2:204634/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAA  >  CP009273/3375115‑3375404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACC  >  CP009273/3375111‑3375408
                                                                                                                                                     |                                                                                                                                                    
CGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCCGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAT                                                                                                                                                      >  SRR19973065.993918/1‑150 (MQ=60)
CGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAT                                                                                                                                                      >  SRR19973065.993895/1‑150 (MQ=60)
CGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAT                                                                                                                                                      >  SRR19973065.1692121/1‑150 (MQ=60)
  GGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATAT                                                                                                                                                    <  SRR19973065.2868414/150‑1 (MQ=60)
    CACGAAAGTGAAGCTTGGCGTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGG                                                                                                                                                  <  SRR19973065.172556/150‑1 (MQ=60)
    CACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGG                                                                                                                                                  >  SRR19973065.3533866/1‑150 (MQ=60)
    CACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGG                                                                                                                                                  >  SRR19973065.3534092/1‑150 (MQ=60)
    CACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGG                                                                                                                                                  >  SRR19973065.1676713/1‑150 (MQ=60)
    CACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGG                                                                                                                                                  >  SRR19973065.1633740/1‑150 (MQ=60)
       GAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGG                                                                                                                                               <  SRR19973065.622514/150‑1 (MQ=60)
         AAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCA                                                                                                                                             >  SRR19973065.887681/1‑150 (MQ=60)
         AAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCA                                                                                                                                             >  SRR19973065.888016/1‑150 (MQ=60)
          AGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAACTGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAA                                                                                                                                            >  SRR19973065.3471880/1‑150 (MQ=60)
          AGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCCCGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAA                                                                                                                                            >  SRR19973065.3471882/1‑150 (MQ=60)
          AGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAA                                                                                                                                            >  SRR19973065.1150002/1‑150 (MQ=60)
             GAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGG                                                                                                                                         <  SRR19973065.2922005/150‑1 (MQ=60)
                GCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTAT                                                                                                                                      <  SRR19973065.2885384/150‑1 (MQ=60)
                GCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTAT                                                                                                                                      <  SRR19973065.2380588/150‑1 (MQ=60)
                    GGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAA                                                                                                                                  >  SRR19973065.3466231/1‑150 (MQ=60)
                       CTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATC                                                                                                                               <  SRR19973065.2260059/150‑1 (MQ=60)
                        TGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAANTGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCG                                                                                                                              <  SRR19973065.1795104/150‑1 (MQ=60)
                        TGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAANTGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCG                                                                                                                              <  SRR19973065.1795117/150‑1 (MQ=60)
                        TGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCG                                                                                                                              <  SRR19973065.1450827/150‑1 (MQ=60)
                         GCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGA                                                                                                                             <  SRR19973065.723803/150‑1 (MQ=60)
                          CTGCTTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATAACAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGAT                                                                                                                            <  SRR19973065.2016865/150‑1 (MQ=60)
                           TGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATG                                                                                                                           <  SRR19973065.1985234/150‑1 (MQ=60)
                               TAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGT                                                                                                                       <  SRR19973065.3202095/150‑1 (MQ=60)
                                AACGTCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATTAAGTT                                                                                                                      <  SRR19973065.3363650/150‑1 (MQ=60)
                                AACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAACTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTT                                                                                                                      <  SRR19973065.1518051/150‑1 (MQ=60)
                                 ACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTG                                                                                                                     <  SRR19973065.2294797/150‑1 (MQ=60)
                                  CGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGTCGGCAAAGGTATTAAAATCGATGAAGTTGT                                                                                                                    >  SRR19973065.2180133/1‑150 (MQ=60)
                                  CGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGT                                                                                                                    >  SRR19973065.2180045/1‑150 (MQ=60)
                                  CGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGT                                                                                                                    >  SRR19973065.2180191/1‑150 (MQ=60)
                                   GGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTC                                                                                                                   <  SRR19973065.760702/150‑1 (MQ=60)
                                   GGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTC                                                                                                                   <  SRR19973065.760277/150‑1 (MQ=60)
                                      AAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCCGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGATCGATGGTCAGCTAAAATATTGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAA                                                                                                                >  SRR19973065.3421769/1‑150 (MQ=60)
                                      AAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAA                                                                                                                >  SRR19973065.3421726/1‑150 (MQ=60)
                                      AAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAA                                                                                                                <  SRR19973065.694082/150‑1 (MQ=60)
                                       AACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAG                                                                                                               <  SRR19973065.1127523/150‑1 (MQ=60)
                                       AACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAG                                                                                                               <  SRR19973065.1534165/150‑1 (MQ=60)
                                       AACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAG                                                                                                               <  SRR19973065.1127537/150‑1 (MQ=60)
                                       AACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAG                                                                                                               <  SRR19973065.1127413/150‑1 (MQ=60)
                                         CCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGA                                                                                                             >  SRR19973065.353109/1‑150 (MQ=60)
                                             TGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCC                                                                                                         >  SRR19973065.2506289/1‑150 (MQ=60)
                                                 AGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCGGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGC                                                                                                     >  SRR19973065.1956258/1‑150 (MQ=60)
                                                   TAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTG                                                                                                   >  SRR19973065.2152151/1‑150 (MQ=60)
                                                   TAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTG                                                                                                   <  SRR19973065.522574/150‑1 (MQ=60)
                                                         TGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGACGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGG                                                                                             >  SRR19973065.2189002/1‑150 (MQ=60)
                                                         TGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGG                                                                                             >  SRR19973065.2188467/1‑150 (MQ=60)
                                                           ACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCT                                                                                           <  SRR19973065.2862360/150‑1 (MQ=60)
                                                            CGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTG                                                                                          <  SRR19973065.1790664/150‑1 (MQ=60)
                                                                CGGTACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTT                                                                                      <  SRR19973065.534370/150‑1 (MQ=60)
                                                                   TACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGCTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCA                                                                                   <  SRR19973065.3295533/150‑1 (MQ=60)
                                                                    ACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCGGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAA                                                                                  >  SRR19973065.92918/1‑150 (MQ=60)
                                                                      CAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAA                                                                                >  SRR19973065.2730783/1‑150 (MQ=60)
                                                                      CAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAA                                                                                >  SRR19973065.1099441/1‑150 (MQ=60)
                                                                        GCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAG                                                                              >  SRR19973065.2726359/1‑150 (MQ=60)
                                                                          ACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGAC                                                                            <  SRR19973065.3075298/150‑1 (MQ=60)
                                                                           CCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACG                                                                           >  SRR19973065.267935/1‑150 (MQ=60)
                                                                             TCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGAT                                                                         <  SRR19973065.758950/150‑1 (MQ=60)
                                                                             TCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGAT                                                                         <  SRR19973065.758306/150‑1 (MQ=60)
                                                                             TCGTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGAT                                                                         <  SRR19973065.758970/150‑1 (MQ=60)
                                                                               TTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGT                                                                       >  SRR19973065.588012/1‑150 (MQ=60)
                                                                                TCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTG                                                                      <  SRR19973065.290962/150‑1 (MQ=60)
                                                                                TCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTG                                                                      <  SRR19973065.2073362/150‑1 (MQ=60)
                                                                                  GTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGAT                                                                    <  SRR19973065.3025623/150‑1 (MQ=60)
                                                                                  GTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGAT                                                                    <  SRR19973065.368173/150‑1 (MQ=60)
                                                                                   TCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGTAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATC                                                                   >  SRR19973065.620772/1‑150 (MQ=60)
                                                                                     GGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCAT                                                                 <  SRR19973065.795414/150‑1 (MQ=60)
                                                                                     GGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCAT                                                                 <  SRR19973065.795422/150‑1 (MQ=60)
                                                                                        CAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGG                                                              <  SRR19973065.109782/150‑1 (MQ=60)
                                                                                             CTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCA                                                         >  SRR19973065.2733918/1‑150 (MQ=60)
                                                                                               GAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAAC                                                       <  SRR19973065.591919/150‑1 (MQ=60)
                                                                                               GAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAAC                                                       <  SRR19973065.838042/150‑1 (MQ=60)
                                                                                               GAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAAC                                                       <  SRR19973065.591808/150‑1 (MQ=60)
                                                                                               GAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAAC                                                       <  SRR19973065.3329991/150‑1 (MQ=60)
                                                                                                  ATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGC                                                    >  SRR19973065.3072831/1‑150 (MQ=60)
                                                                                                     ATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGAT                                                 <  SRR19973065.75222/150‑1 (MQ=60)
                                                                                                     ATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGAT                                                 <  SRR19973065.3299712/150‑1 (MQ=60)
                                                                                                     ATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGAT                                                 <  SRR19973065.3299262/150‑1 (MQ=60)
                                                                                                       CACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGANGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCG                                               >  SRR19973065.1792792/1‑150 (MQ=60)
                                                                                                       CACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGANGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCG                                               >  SRR19973065.1791830/1‑150 (MQ=60)
                                                                                                       CACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCG                                               >  SRR19973065.2844225/1‑150 (MQ=60)
                                                                                                       CACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCG                                               >  SRR19973065.1085012/1‑150 (MQ=60)
                                                                                                       CACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCA                                               >  SRR19973065.2480430/1‑150 (MQ=60)
                                                                                                       CACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCA                                               >  SRR19973065.2479366/1‑150 (MQ=60)
                                                                                                        ACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGG                                              <  SRR19973065.802701/150‑1 (MQ=60)
                                                                                                        ACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGG                                              <  SRR19973065.1123765/150‑1 (MQ=60)
                                                                                                         CGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGG                                             <  SRR19973065.178470/150‑1 (MQ=60)
                                                                                                              GCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAAC                                        <  SRR19973065.1715659/150‑1 (MQ=60)
                                                                                                                  TGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGA                                    >  SRR19973065.1333702/1‑150 (MQ=60)
                                                                                                                   GGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGAT                                   <  SRR19973065.2845922/150‑1 (MQ=60)
                                                                                                                      AGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGC                                >  SRR19973065.2764665/1‑150 (MQ=60)
                                                                                                                       GGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACTATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCT                               >  SRR19973065.2646239/1‑150 (MQ=60)
                                                                                                                       GGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACTATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCT                               >  SRR19973065.33176/1‑150 (MQ=60)
                                                                                                                       GGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACTATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCT                               >  SRR19973065.32999/1‑150 (MQ=60)
                                                                                                                       GGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACTATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCT                               >  SRR19973065.33175/1‑150 (MQ=60)
                                                                                                                       GGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCT                               >  SRR19973065.2915892/1‑150 (MQ=60)
                                                                                                                       GGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCT                               <  SRR19973065.1209006/150‑1 (MQ=60)
                                                                                                                          GCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAA                            >  SRR19973065.3412061/1‑150 (MQ=60)
                                                                                                                           CAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAA                           >  SRR19973065.1613084/1‑150 (MQ=60)
                                                                                                                           CAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAA                           >  SRR19973065.1953445/1‑150 (MQ=60)
                                                                                                                            AACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAAT                          >  SRR19973065.1836203/1‑150 (MQ=60)
                                                                                                                               GTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCG                       <  SRR19973065.1211846/150‑1 (MQ=60)
                                                                                                                                TTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGT                      >  SRR19973065.434119/1‑150 (MQ=60)
                                                                                                                                        TGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCT              <  SRR19973065.1306036/150‑1 (MQ=60)
                                                                                                                                               CTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCA       <  SRR19973065.1363845/150‑1 (MQ=60)
                                                                                                                                                TAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAA      >  SRR19973065.1702375/1‑150 (MQ=60)
                                                                                                                                                TAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAA      >  SRR19973065.940565/1‑150 (MQ=60)
                                                                                                                                                 AAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAA     >  SRR19973065.2355733/1‑150 (MQ=60)
                                                                                                                                                   AATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAAC   >  SRR19973065.3511211/1‑150 (MQ=60)
                                                                                                                                                    ATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACC  >  SRR19973065.3210348/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACC  >  CP009273/3375111‑3375408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑