Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,375,260 G→T 100% D387Y (GAT→TAT)  degQ → serine endoprotease, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,375,2600GT99.5% 623.0 / ‑4.1 192D387Y (GAT→TAT) degQserine endoprotease, periplasmic
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (88/103);  total (88/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 195 total aligned reads displayed.

CACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCA  >  CP009273/3375115‑3375403
                                                                                                                                                 |                                                                                                                                               
cACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAatat                                                                                                                                               <  2:1941557/148‑1 (MQ=255)
 aCGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACGAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCATCTAAAATATg                                                                                                                                              <  1:2508641/148‑1 (MQ=255)
 aCGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATg                                                                                                                                              <  1:468274/148‑1 (MQ=255)
   gAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATggc                                                                                                                                            >  1:1175255/1‑148 (MQ=255)
       gTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCa                                                                                                                                        <  1:1007978/148‑1 (MQ=255)
         gAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCaaa                                                                                                                                      <  2:3759607/148‑1 (MQ=255)
           aGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAgg                                                                                                                                    <  2:2322218/148‑1 (MQ=255)
            gCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGt                                                                                                                                   >  2:1205687/1‑148 (MQ=255)
               tGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTAtt                                                                                                                                >  1:260994/1‑148 (MQ=255)
               tGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTAtt                                                                                                                                <  1:3530068/148‑1 (MQ=255)
                 gCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTaa                                                                                                                              >  2:1290465/1‑148 (MQ=255)
                    tgctgcGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAAt                                                                                                                           <  2:2653581/148‑1 (MQ=255)
                       tgcGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGa                                                                                                                        <  1:3633261/148‑1 (MQ=255)
                       tgcGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGa                                                                                                                        <  2:2618049/148‑1 (MQ=255)
                       tgcGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGa                                                                                                                        <  2:866814/148‑1 (MQ=255)
                            aaCGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAg                                                                                                                   <  2:196441/148‑1 (MQ=255)
                             aCGGCAAACCACTGGAAGTAGAAGTGACGCTCGATAGCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACNCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGt                                                                                                                  >  1:1933310/1‑148 (MQ=255)
                             aCGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGt                                                                                                                  >  2:2123828/1‑148 (MQ=255)
                              cGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGtt                                                                                                                 >  2:3508213/1‑148 (MQ=255)
                                   aaCCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCaa                                                                                                            <  2:2783355/148‑1 (MQ=255)
                                    aCCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCACAGGTATTAAAATCGATGAAGTTGTCaaa                                                                                                           >  1:701617/1‑148 (MQ=255)
                                    aCCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCaaa                                                                                                           >  1:520037/1‑148 (MQ=255)
                                    aCCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCaaa                                                                                                           >  1:1666409/1‑148 (MQ=255)
                                      cACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAgg                                                                                                         <  2:1926156/148‑1 (MQ=255)
                                          ggAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGc                                                                                                     <  2:3029618/148‑1 (MQ=255)
                                              gTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAg                                                                                                 <  1:3230626/148‑1 (MQ=255)
                                              gTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAg                                                                                                 <  1:1413638/148‑1 (MQ=255)
                                               tAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAgc                                                                                                >  2:2901137/1‑148 (MQ=255)
                                                 gAAGTGACGCTCGATACCAGCACCTCTACGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAgctg                                                                                              <  2:2165512/148‑1 (MQ=255)
                                                  aaGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAgctgc                                                                                             <  1:22471/148‑1 (MQ=255)
                                                    gTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTc                                                                                           <  2:1315762/148‑1 (MQ=255)
                                                       aCGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAgg                                                                                        >  2:2797741/1‑148 (MQ=255)
                                                       aCGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAgg                                                                                        <  2:804436/148‑1 (MQ=255)
                                                           tCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAggctgg                                                                                    <  2:1634041/148‑1 (MQ=255)
                                                             gATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAggctggct                                                                                  <  2:53028/148‑1 (MQ=255)
                                                              aTACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCtt                                                                                 >  1:1527001/1‑148 (MQ=255)
                                                              aTACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCtt                                                                                 >  1:3427092/1‑148 (MQ=255)
                                                                   aGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCaaa                                                                            >  1:2315692/1‑148 (MQ=255)
                                                                    gCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCaaaa                                                                           <  1:3230217/148‑1 (MQ=255)
                                                                    gCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCaaaa                                                                           >  1:2653020/1‑148 (MQ=255)
                                                                    gCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCaaaa                                                                           <  1:347004/148‑1 (MQ=255)
                                                                     cACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCaaaaa                                                                          >  1:1463881/1‑148 (MQ=255)
                                                                      aCCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAg                                                                         >  1:509010/1‑148 (MQ=255)
                                                                       ccTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGa                                                                        >  2:573373/1‑148 (MQ=255)
                                                                        ctattCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGAc                                                                       <  2:1810131/145‑1 (MQ=255)
                                                                         tctTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACg                                                                      <  2:1241818/148‑1 (MQ=255)
                                                                            tcgtcgGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGAtg                                                                   <  2:1016622/148‑1 (MQ=255)
                                                                             cgtcgGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGAtgt                                                                  <  1:3392249/148‑1 (MQ=255)
                                                                               tcgGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGa                                                                <  1:79031/148‑1 (MQ=255)
                                                                                 ggCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAATGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATc                                                              <  2:2144096/148‑1 (MQ=255)
                                                                                   ccAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCAt                                                            <  1:276834/148‑1 (MQ=255)
                                                                                   ccAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCAt                                                            >  2:3800746/1‑148 (MQ=255)
                                                                                    cAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCAtt                                                           >  1:3138903/1‑148 (MQ=255)
                                                                                    cAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCAtt                                                           <  2:260994/148‑1 (MQ=255)
                                                                                     aGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTg                                                          <  2:2861736/148‑1 (MQ=255)
                                                                                       cgcTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATGAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGc                                                        >  1:3507106/1‑148 (MQ=255)
                                                                                          tGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTc                                                     >  1:2692000/1‑148 (MQ=255)
                                                                                          tGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTc                                                     >  1:222685/1‑148 (MQ=255)
                                                                                           gAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCa                                                    <  1:537499/148‑1 (MQ=255)
                                                                                           gAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCa                                                    >  1:2952407/1‑148 (MQ=255)
                                                                                           gAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCa                                                    >  1:2952832/1‑148 (MQ=255)
                                                                                           gAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCa                                                    >  1:3300796/1‑148 (MQ=255)
                                                                                           gAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCa                                                    >  1:1885719/1‑148 (MQ=255)
                                                                                            aaaTGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCaa                                                   >  2:1405391/1‑148 (MQ=255)
                                                                                            aaaTGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCAAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCaa                                                   >  2:1681164/1‑148 (MQ=255)
                                                                                              aTGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAAcc                                                 >  1:1043624/1‑148 (MQ=255)
                                                                                                 aTCACGCCAGCTCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACcgcg                                              <  1:2731757/148‑1 (MQ=255)
                                                                                                  tCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGa                                             <  2:1067246/148‑1 (MQ=255)
                                                                                                  tCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGa                                             <  2:3578813/148‑1 (MQ=255)
                                                                                                     cGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCg                                          <  1:2923286/148‑1 (MQ=255)
                                                                                                     cGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCg                                          <  1:37207/148‑1 (MQ=255)
                                                                                                       ccAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCggg                                        <  2:1962314/148‑1 (MQ=255)
                                                                                                       ccAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCggg                                        <  2:1773097/148‑1 (MQ=255)
                                                                                                       ccAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCggg                                        <  2:1480497/148‑1 (MQ=255)
                                                                                                        cAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGt                                       >  2:3732099/1‑148 (MQ=255)
                                                                                                               ggAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCg                                <  1:1767823/148‑1 (MQ=255)
                                                                                                                gAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGa                               <  1:2711440/148‑1 (MQ=255)
                                                                                                                gAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGa                               >  1:3576744/1‑148 (MQ=255)
                                                                                                                gAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGa                               >  1:1264579/1‑148 (MQ=255)
                                                                                                                gAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGa                               <  2:1322735/148‑1 (MQ=255)
                                                                                                                 aaGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGAt                              <  2:2315692/148‑1 (MQ=255)
                                                                                                                   ggTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTg                            <  1:1205742/148‑1 (MQ=255)
                                                                                                                   ggTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTg                            >  1:28172/1‑148 (MQ=255)
                                                                                                                   ggTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTg                            >  1:3098174/1‑148 (MQ=255)
                                                                                                                     tGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCt                          <  2:101322/148‑1 (MQ=255)
                                                                                                                        aaCGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGaa                       <  1:1546587/148‑1 (MQ=255)
                                                                                                                         aCGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGaaa                      <  1:3667167/148‑1 (MQ=255)
                                                                                                                         aCGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGaaa                      <  2:765779/148‑1 (MQ=255)
                                                                                                                            ttGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGc                   >  2:3428728/1‑148 (MQ=255)
                                                                                                                             tGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCg                  >  1:1343551/1‑148 (MQ=255)
                                                                                                                                gCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTaa               <  1:402350/148‑1 (MQ=255)
                                                                                                                                   aTGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGt            >  1:2115525/1‑148 (MQ=255)
                                                                                                                                    tGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTg           <  1:3771285/148‑1 (MQ=255)
                                                                                                                                     ggTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGc          >  2:1901427/1‑148 (MQ=255)
                                                                                                                                      gTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCt         >  1:3346096/1‑148 (MQ=255)
                                                                                                                                      gTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCt         <  1:2375215/148‑1 (MQ=255)
                                                                                                                                         aGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTggc      >  1:589768/1‑148 (MQ=255)
                                                                                                                                           cTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTggcgg    >  2:210717/1‑148 (MQ=255)
                                                                                                                                             aaaaTATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCa  >  2:2583506/1‑148 (MQ=255)
                                                                                                                                             aaaaTATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCa  >  2:1808944/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCA  >  CP009273/3375115‑3375403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAAC  >  CP009273/3375111‑3375407
                                                                                                                                                     |                                                                                                                                                   
CGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAT                                                                                                                                                     >  SRR19973069.771182/1‑150 (MQ=60)
    CACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGG                                                                                                                                                 <  SRR19973069.2749787/150‑1 (MQ=60)
     ACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACGAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCATCTAAAATATGGC                                                                                                                                                <  SRR19973069.2525363/150‑1 (MQ=60)
     ACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGACGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGC                                                                                                                                                >  SRR19973069.1183393/1‑150 (MQ=60)
     ACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGC                                                                                                                                                <  SRR19973069.471689/150‑1 (MQ=60)
          AGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCCA                                                                                                                                           >  SRR19973069.3732534/1‑150 (MQ=60)
           GTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAA                                                                                                                                          <  SRR19973069.1014917/150‑1 (MQ=60)
                 CTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATT                                                                                                                                    >  SRR19973069.262873/1‑150 (MQ=60)
                 CTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATT                                                                                                                                    <  SRR19973069.1562958/150‑1 (MQ=60)
                 CTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATT                                                                                                                                    <  SRR19973069.3472346/150‑1 (MQ=60)
                   TGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAA                                                                                                                                  <  SRR19973069.3552921/150‑1 (MQ=60)
                           TGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATG                                                                                                                          <  SRR19973069.3657014/150‑1 (MQ=60)
                            GCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGA                                                                                                                         <  SRR19973069.655653/150‑1 (MQ=60)
                            GCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGA                                                                                                                         <  SRR19973069.564572/150‑1 (MQ=60)
                               TAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATAGCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACNCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGT                                                                                                                      >  SRR19973069.1946583/1‑150 (MQ=60)
                               TAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGT                                                                                                                      >  SRR19973069.3499450/1‑150 (MQ=60)
                                AACGGCAAACCACTGGATGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTT                                                                                                                     <  SRR19973069.2780400/150‑1 (MQ=60)
                                  CGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGT                                                                                                                   <  SRR19973069.140376/150‑1 (MQ=60)
                                      AAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCACAGGTATTAAAATCGATGAAGTTGTCAAA                                                                                                               >  SRR19973069.706550/1‑150 (MQ=60)
                                      AAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAA                                                                                                               >  SRR19973069.523729/1‑150 (MQ=60)
                                      AAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAA                                                                                                               >  SRR19973069.1677964/1‑150 (MQ=60)
                                      AAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAA                                                                                                               >  SRR19973069.706680/1‑150 (MQ=60)
                                           ACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAG                                                                                                          <  SRR19973069.3612056/150‑1 (MQ=60)
                                           ACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAG                                                                                                          <  SRR19973069.1249326/150‑1 (MQ=60)
                                              GGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCC                                                                                                       <  SRR19973069.2259233/150‑1 (MQ=60)
                                                  GTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCT                                                                                                   <  SRR19973069.3251755/150‑1 (MQ=60)
                                                  GTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCT                                                                                                   <  SRR19973069.1774450/150‑1 (MQ=60)
                                                  GTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCT                                                                                                   <  SRR19973069.1423489/150‑1 (MQ=60)
                                                      AAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTC                                                                                               <  SRR19973069.22797/150‑1 (MQ=60)
                                                         TGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGG                                                                                            >  SRR19973069.3661786/1‑150 (MQ=60)
                                                          GACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGC                                                                                           >  SRR19973069.206335/1‑150 (MQ=60)
                                                           ACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGTTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCT                                                                                          <  SRR19973069.2718830/150‑1 (MQ=60)
                                                              CTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGC                                                                                       >  SRR19973069.3419491/1‑150 (MQ=60)
                                                                CGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTT                                                                                     >  SRR19973069.3449379/1‑150 (MQ=60)
                                                                CGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTT                                                                                     >  SRR19973069.1537510/1‑150 (MQ=60)
                                                                 GATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTG                                                                                    <  SRR19973069.451937/150‑1 (MQ=60)
                                                                     CCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAA                                                                                >  SRR19973069.2331454/1‑150 (MQ=60)
                                                                      CAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAA                                                                               >  SRR19973069.2670796/1‑150 (MQ=60)
                                                                       AGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAA                                                                              >  SRR19973069.1474098/1‑150 (MQ=60)
                                                                        GCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAG                                                                             <  SRR19973069.3251343/150‑1 (MQ=60)
                                                                        GCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAG                                                                             <  SRR19973069.349476/150‑1 (MQ=60)
                                                                        GCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAG                                                                             >  SRR19973069.513485/1‑150 (MQ=60)
                                                                        GCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAG                                                                             <  SRR19973069.47920/150‑1 (MQ=60)
                                                                        GCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAG                                                                             >  SRR19973069.512637/1‑150 (MQ=60)
                                                                            CTCTTCGTCGGCCAGCGCTGAAATTATCACCCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATAGATGAAGTTTTAAAAGGAAGCCCAGCTGCACAGGCTGGCTTGCAAAAAGAAGA                                                                         >  SRR19973069.3308936/1‑150 (MQ=60)
                                                                              CTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAATTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATG                                                                       >  SRR19973069.2360675/1‑150 (MQ=60)
                                                                              CTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATG                                                                       >  SRR19973069.3154271/1‑150 (MQ=60)
                                                                                 CGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGA                                                                    <  SRR19973069.3414401/150‑1 (MQ=60)
                                                                                 CGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGA                                                                    <  SRR19973069.1501526/150‑1 (MQ=60)
                                                                                   TCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATC                                                                  >  SRR19973069.1794460/1‑150 (MQ=60)
                                                                                   TCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATC                                                                  <  SRR19973069.79892/150‑1 (MQ=60)
                                                                                    CGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCA                                                                 >  SRR19973069.2708246/1‑150 (MQ=60)
                                                                                      GCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATT                                                               >  SRR19973069.3159557/1‑150 (MQ=60)
                                                                                      GCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATT                                                               >  SRR19973069.2538790/1‑150 (MQ=60)
                                                                                      GCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATT                                                               >  SRR19973069.3159461/1‑150 (MQ=60)
                                                                                       CCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTG                                                              <  SRR19973069.278782/150‑1 (MQ=60)
                                                                                       CCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTG                                                              <  SRR19973069.39339/150‑1 (MQ=60)
                                                                                       CCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTG                                                              <  SRR19973069.3548904/150‑1 (MQ=60)
                                                                                         AGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATGAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGC                                                            >  SRR19973069.3529673/1‑150 (MQ=60)
                                                                                           CGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGT                                                          >  SRR19973069.868644/1‑150 (MQ=60)
                                                                                            GCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTC                                                         >  SRR19973069.2710039/1‑150 (MQ=60)
                                                                                            GCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTC                                                         >  SRR19973069.224404/1‑150 (MQ=60)
                                                                                             CTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCA                                                        >  SRR19973069.2972432/1‑150 (MQ=60)
                                                                                             CTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCA                                                        >  SRR19973069.3322412/1‑150 (MQ=60)
                                                                                             CTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCA                                                        >  SRR19973069.2972425/1‑150 (MQ=60)
                                                                                             CTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCA                                                        >  SRR19973069.2972001/1‑150 (MQ=60)
                                                                                             CTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCA                                                        >  SRR19973069.2971997/1‑150 (MQ=60)
                                                                                             CTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCA                                                        >  SRR19973069.1898811/1‑150 (MQ=60)
                                                                                             CTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGACCAGCTGCTCAGGCTGGCTTGCAAAAAGACGAAGTGATCATTGGCGTCA                                                        >  SRR19973069.2971961/1‑150 (MQ=60)
                                                                                               GAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAAC                                                      <  SRR19973069.541287/150‑1 (MQ=60)
                                                                                                AAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACC                                                     >  SRR19973069.1050874/1‑150 (MQ=60)
                                                                                                   TGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCTACCGCG                                                  >  SRR19973069.3251558/1‑150 (MQ=60)
                                                                                                     ATCACGCCAGCTCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGAT                                                <  SRR19973069.2750043/150‑1 (MQ=60)
                                                                                                     ATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGAT                                                >  SRR19973069.2525199/1‑150 (MQ=60)
                                                                                                        ACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGG                                             <  SRR19973069.533782/150‑1 (MQ=60)
                                                                                                         CGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGG                                            <  SRR19973069.37712/150‑1 (MQ=60)
                                                                                                         CGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGG                                            <  SRR19973069.2942652/150‑1 (MQ=60)
                                                                                                            CAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGA                                         <  SRR19973069.3766537/150‑1 (MQ=60)
                                                                                                               CGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACT                                      <  SRR19973069.2320201/150‑1 (MQ=60)
                                                                                                                  TGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGA                                   >  SRR19973069.3600066/1‑150 (MQ=60)
                                                                                                                  TGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGA                                   >  SRR19973069.1273095/1‑150 (MQ=60)
                                                                                                                   GGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGAT                                  <  SRR19973069.1780280/150‑1 (MQ=60)
                                                                                                                    GAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATT                                 <  SRR19973069.2729621/150‑1 (MQ=60)
                                                                                                                     AAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTG                                >  SRR19973069.28582/1‑150 (MQ=60)
                                                                                                                     AAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTG                                >  SRR19973069.3118587/1‑150 (MQ=60)
                                                                                                                       GGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCT                              <  SRR19973069.1299099/150‑1 (MQ=60)
                                                                                                                       GGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCT                              <  SRR19973069.1214028/150‑1 (MQ=60)
                                                                                                                       GGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCT                              <  SRR19973069.1213973/150‑1 (MQ=60)
                                                                                                                           CAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAA                          >  SRR19973069.3387046/1‑150 (MQ=60)
                                                                                                                            AACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAAT                         <  SRR19973069.218607/150‑1 (MQ=60)
                                                                                                                            AACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAAT                         >  SRR19973069.11694/1‑150 (MQ=60)
                                                                                                                            AACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAAT                         <  SRR19973069.1557167/150‑1 (MQ=60)
                                                                                                                            AACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAAT                         <  SRR19973069.1557130/150‑1 (MQ=60)
                                                                                                                             ACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATG                        <  SRR19973069.3691165/150‑1 (MQ=60)
                                                                                                                               GTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCG                      >  SRR19973069.1352841/1‑150 (MQ=60)
                                                                                                                                    GCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAG                 <  SRR19973069.405300/150‑1 (MQ=60)
                                                                                                                                     CGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGT                >  SRR19973069.2130108/1‑150 (MQ=60)
                                                                                                                                        TGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCT             <  SRR19973069.3795780/150‑1 (MQ=60)
                                                                                                                                        TGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCT             >  SRR19973069.3367995/1‑150 (MQ=60)
                                                                                                                                        TGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCT             >  SRR19973069.3367994/1‑150 (MQ=60)
                                                                                                                                        TGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCT             <  SRR19973069.2920298/150‑1 (MQ=60)
                                                                                                                                        TGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCT             <  SRR19973069.2816326/150‑1 (MQ=60)
                                                                                                                                         GGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTG            <  SRR19973069.1932270/150‑1 (MQ=60)
                                                                                                                                          GTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGG           <  SRR19973069.2391327/150‑1 (MQ=60)
                                                                                                                                           TCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGC          >  SRR19973069.593788/1‑150 (MQ=60)
                                                                                                                                           TCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGC          <  SRR19973069.1470539/150‑1 (MQ=60)
                                                                                                                                                  AAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAA   >  SRR19973069.3606788/1‑150 (MQ=60)
                                                                                                                                                   AATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAAC  <  SRR19973069.145374/150‑1 (MQ=60)
                                                                                                                                                   AATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAAC  <  SRR19973069.145433/150‑1 (MQ=60)
                                                                                                                                                   AATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAAC  >  SRR19973069.246405/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
CGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAAC  >  CP009273/3375111‑3375407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑