Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,375,260 G→T 93.8% D387Y (GAT→TAT)  degQ → serine endoprotease, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,375,2600GT93.8% 634.2 / 29.5 195D387Y (GAT→TAT) degQserine endoprotease, periplasmic
Reads supporting (aligned to +/- strand):  ref base G (6/6);  new base T (88/95);  total (94/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

Only 100 of 204 total aligned reads displayed.

GGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACC  >  CP009273/3375113‑3375408
                                                                                                                                                   |                                                                                                                                                    
ggCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAatat                                                                                                                                                    <  1:3538825/150‑1 (MQ=255)
ggCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAatat                                                                                                                                                    <  2:1042144/150‑1 (MQ=255)
 gCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATg                                                                                                                                                   <  2:1586423/150‑1 (MQ=255)
 gCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATg                                                                                                                                                   <  2:2766913/150‑1 (MQ=255)
    cGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGACGGTGCAACGTTGAGCGATGGTCAGCTAAAATATggcg                                                                                                                                                >  2:4588700/1‑150 (MQ=255)
    cGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAatat                                                                                                                                                    <  2:2753124/146‑1 (MQ=255)
    cGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAatat                                                                                                                                                    >  1:2753124/1‑146 (MQ=255)
        aGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGACGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCGGGAAGGTGCAACGTTGAGCGATGGTCCGCTAAAATATGGCGGCaa                                                                                                                                            >  1:3149372/1‑150 (MQ=255)
        aGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCaa                                                                                                                                            <  1:977618/150‑1 (MQ=255)
          tGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAg                                                                                                                                          <  2:2923083/150‑1 (MQ=255)
            aaGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGt                                                                                                                                        <  2:2438104/150‑1 (MQ=255)
            aaGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGt                                                                                                                                        >  2:1815702/1‑150 (MQ=255)
             aGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTa                                                                                                                                       <  1:617361/150‑1 (MQ=255)
               cTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTAtt                                                                                                                                     <  2:2561272/150‑1 (MQ=255)
               cTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTAtt                                                                                                                                     <  2:2526554/150‑1 (MQ=255)
               cTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTAtt                                                                                                                                     <  2:1071789/150‑1 (MQ=255)
               cTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTAtt                                                                                                                                     <  1:3370671/150‑1 (MQ=255)
                ttGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTa                                                                                                                                    <  1:4602957/150‑1 (MQ=255)
                ttGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTa                                                                                                                                    <  2:2588794/150‑1 (MQ=255)
                 tGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTaa                                                                                                                                   <  2:3218025/150‑1 (MQ=255)
                  ggCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTaaa                                                                                                                                  >  2:1948517/1‑150 (MQ=255)
                  ggCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTaaa                                                                                                                                  <  1:630154/150‑1 (MQ=255)
                    ccTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAAt                                                                                                                                >  1:861493/1‑150 (MQ=255)
                         tgcGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATg                                                                                                                           <  2:2102340/150‑1 (MQ=255)
                            gTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAg                                                                                                                        <  2:3340159/150‑1 (MQ=255)
                              aaCGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGtt                                                                                                                      <  1:3351988/150‑1 (MQ=255)
                              aaCGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACATCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGtt                                                                                                                      >  2:743956/1‑150 (MQ=255)
                                cGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGt                                                                                                                    <  2:491026/150‑1 (MQ=255)
                                  gCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCa                                                                                                                  <  1:522533/150‑1 (MQ=255)
                                   cAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCaa                                                                                                                 <  1:724354/150‑1 (MQ=255)
                                    aaaCCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCaaa                                                                                                                >  2:3163360/1‑150 (MQ=255)
                                    aaaCCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCaaa                                                                                                                <  1:1255369/150‑1 (MQ=255)
                                      aCCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAgg                                                                                                              <  2:2901429/150‑1 (MQ=255)
                                        cACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGTGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGaa                                                                                                            >  2:637402/1‑150 (MQ=255)
                                         aCTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAg                                                                                                           >  2:4568561/1‑150 (MQ=255)
                                            ggAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGccc                                                                                                        >  1:2403725/1‑150 (MQ=255)
                                            ggAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGccc                                                                                                        <  1:2622817/150‑1 (MQ=255)
                                             gAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCa                                                                                                       <  2:1030500/150‑1 (MQ=255)
                                              aaGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAg                                                                                                      >  1:3685774/1‑150 (MQ=255)
                                                gTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAgct                                                                                                    >  2:2462541/1‑150 (MQ=255)
                                                  aGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAgctgc                                                                                                  >  1:373114/1‑150 (MQ=255)
                                                     aGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCa                                                                                               >  2:532312/1‑150 (MQ=255)
                                                           gCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAggctgg                                                                                         >  2:1976109/1‑150 (MQ=255)
                                                              cGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCtt                                                                                      <  2:4348803/150‑1 (MQ=255)
                                                               gATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTg                                                                                     >  1:2816316/1‑150 (MQ=255)
                                                               gATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTg                                                                                     >  1:2092938/1‑150 (MQ=255)
                                                                   ccAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCaaa                                                                                 >  1:748648/1‑150 (MQ=255)
                                                                       cACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGa                                                                             <  1:490320/150‑1 (MQ=255)
                                                                       cACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGa                                                                             <  1:2546124/150‑1 (MQ=255)
                                                                        aCCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGAc                                                                            >  2:2513261/1‑150 (MQ=255)
                                                                        aCCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGAc                                                                            >  1:4874700/1‑150 (MQ=255)
                                                                           tctTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGAt                                                                         <  1:3002870/150‑1 (MQ=255)
                                                                              tcgtcgGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGAtgtg                                                                      >  2:315492/1‑150 (MQ=255)
                                                                                  cgGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCa                                                                  <  2:353172/150‑1 (MQ=255)
                                                                                   ggCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCAt                                                                 <  1:1477514/150‑1 (MQ=255)
                                                                                   ggCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCAt                                                                 >  2:2899370/1‑150 (MQ=255)
                                                                                   ggCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCAt                                                                 <  2:2341633/150‑1 (MQ=255)
                                                                                   ggCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCAt                                                                 <  2:2283863/150‑1 (MQ=255)
                                                                                   ggCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCAt                                                                 <  2:4867012/150‑1 (MQ=255)
                                                                                     ccAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTg                                                               <  1:1940435/150‑1 (MQ=255)
                                                                                      cAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTgg                                                              >  1:4315169/1‑150 (MQ=255)
                                                                                      cAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTgg                                                              <  1:3140493/150‑1 (MQ=255)
                                                                                         cgcTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGt                                                           <  1:2311460/150‑1 (MQ=255)
                                                                                         cgcTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGt                                                           >  1:1336685/1‑150 (MQ=255)
                                                                                          gcTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTc                                                          >  2:2933062/1‑150 (MQ=255)
                                                                                           cTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCa                                                         >  2:2291390/1‑150 (MQ=255)
                                                                                            tGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCaa                                                        >  2:4854221/1‑150 (MQ=255)
                                                                                             gAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAAc                                                       <  1:3280040/150‑1 (MQ=255)
                                                                                              aaaTGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAAcc                                                      <  2:410977/150‑1 (MQ=255)
                                                                                              aaaTGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAAcc                                                      >  1:1852387/1‑150 (MQ=255)
                                                                                              aaaTGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAAcc                                                      >  2:4780360/1‑150 (MQ=255)
                                                                                                  gATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGa                                                  >  2:1286489/1‑150 (MQ=255)
                                                                                                    tCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATc                                                <  1:4713271/150‑1 (MQ=255)
                                                                                                    tCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATc                                                <  2:2679482/150‑1 (MQ=255)
                                                                                                      aCGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCgg                                              <  2:4006348/150‑1 (MQ=255)
                                                                                                      aCGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCgg                                              >  2:4708790/1‑150 (MQ=255)
                                                                                                         ccAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTg                                           >  2:2789809/1‑150 (MQ=255)
                                                                                                          cAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGa                                          <  1:1025470/150‑1 (MQ=255)
                                                                                                           aGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCACAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGaa                                         >  2:3123562/1‑150 (MQ=255)
                                                                                                           aGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGaa                                         >  1:3833914/1‑150 (MQ=255)
                                                                                                           aGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATAGGCGTCAACCGCGATCGGGTGaa                                         >  2:3442747/1‑150 (MQ=255)
                                                                                                               cTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCg                                     >  2:660510/1‑150 (MQ=255)
                                                                                                               cTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCg                                     >  1:2250694/1‑150 (MQ=255)
                                                                                                                 ggAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGAt                                   <  2:1407984/150‑1 (MQ=255)
                                                                                                                  gAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGAtt                                  <  2:373114/150‑1 (MQ=255)
                                                                                                                  gAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGAtt                                  <  1:4124998/150‑1 (MQ=255)
                                                                                                                   aaGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTg                                 <  2:4309425/150‑1 (MQ=255)
                                                                                                                      gTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTg                              <  1:4052149/150‑1 (MQ=255)
                                                                                                                          aaCGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAAt                          >  1:4521418/1‑150 (MQ=255)
                                                                                                                          aaCGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAAt                          <  1:2016253/150‑1 (MQ=255)
                                                                                                                            cGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGc                        <  1:2047762/150‑1 (MQ=255)
                                                                                                                             gTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCg                       >  2:4521148/1‑150 (MQ=255)
                                                                                                                                  gCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAg                  <  2:5006337/150‑1 (MQ=255)
                                                                                                                                    gATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTg                >  1:157129/1‑150 (MQ=255)
                                                                                                                                    gATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTg                >  1:4597542/1‑150 (MQ=255)
                                                                                                                                     aTGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGc               >  1:1545119/1‑150 (MQ=255)
                                                                                                                                       ggTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTg             >  1:394091/1‑150 (MQ=255)
                                                                                                                                          cAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTggcg          >  1:4467903/1‑150 (MQ=255)
                                                                                                                                          cAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTggcg          <  1:1414014/150‑1 (MQ=255)
                                                                                                                                               aaaaTATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCaaa     >  2:2847397/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACC  >  CP009273/3375113‑3375408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCG  >  CP009273/3375111‑3375409
                                                                                                                                                     |                                                                                                                                                     
CGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAT                                                                                                                                                       >  SRR19973083.4829032/1‑150 (MQ=60)
CGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAT                                                                                                                                                       >  SRR19973083.4049089/1‑150 (MQ=60)
CGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAT                                                                                                                                                       >  SRR19973083.3405025/1‑150 (MQ=60)
CGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAT                                                                                                                                                       >  SRR19973083.3404970/1‑150 (MQ=60)
CGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAT                                                                                                                                                       <  SRR19973083.1678352/150‑1 (MQ=60)
 GGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATA                                                                                                                                                      <  SRR19973083.4958539/150‑1 (MQ=60)
  GGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATAT                                                                                                                                                     <  SRR19973083.3555071/150‑1 (MQ=60)
  GGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATAT                                                                                                                                                     <  SRR19973083.3325525/150‑1 (MQ=60)
   GCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCGGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATG                                                                                                                                                    >  SRR19973083.1453620/1‑150 (MQ=60)
    CACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGG                                                                                                                                                   <  SRR19973083.702606/150‑1 (MQ=60)
    CACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGG                                                                                                                                                   >  SRR19973083.1857014/1‑150 (MQ=60)
       GAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGG                                                                                                                                                >  SRR19973083.2262799/1‑150 (MQ=60)
        AAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGC                                                                                                                                               >  SRR19973083.3888273/1‑150 (MQ=60)
          AGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGACGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCGGGAAGGTGCAACGTTGAGCGATGGTCCGCTAAAATATGGCGGCAA                                                                                                                                             >  SRR19973083.3164412/1‑150 (MQ=60)
          AGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAA                                                                                                                                             <  SRR19973083.982468/150‑1 (MQ=60)
          AGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAA                                                                                                                                             >  SRR19973083.3162732/1‑150 (MQ=60)
               AGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTA                                                                                                                                        <  SRR19973083.620462/150‑1 (MQ=60)
                 CTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATT                                                                                                                                      <  SRR19973083.3386328/150‑1 (MQ=60)
                 CTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATT                                                                                                                                      <  SRR19973083.772700/150‑1 (MQ=60)
                  TTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTA                                                                                                                                     <  SRR19973083.4622173/150‑1 (MQ=60)
                  TTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTA                                                                                                                                     <  SRR19973083.4622833/150‑1 (MQ=60)
                    GGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAA                                                                                                                                   <  SRR19973083.633316/150‑1 (MQ=60)
                    GGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAA                                                                                                                                   <  SRR19973083.633312/150‑1 (MQ=60)
                      CCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAAT                                                                                                                                 >  SRR19973083.865530/1‑150 (MQ=60)
                            GCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGNTGA                                                                                                                           <  SRR19973083.4477399/150‑1 (MQ=60)
                                AACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTT                                                                                                                       <  SRR19973083.3367605/150‑1 (MQ=60)
                                AACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGGTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTT                                                                                                                       >  SRR19973083.3179139/1‑150 (MQ=60)
                                    GCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCA                                                                                                                   <  SRR19973083.525097/150‑1 (MQ=60)
                                     CAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAA                                                                                                                  <  SRR19973083.727934/150‑1 (MQ=60)
                                      AAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAA                                                                                                                 <  SRR19973083.1261490/150‑1 (MQ=60)
                                      AAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAA                                                                                                                 <  SRR19973083.2466059/150‑1 (MQ=60)
                                              GTAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCC                                                                                                         >  SRR19973083.5105831/1‑150 (MQ=60)
                                              GGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCC                                                                                                         <  SRR19973083.2635981/150‑1 (MQ=60)
                                              GGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCC                                                                                                         >  SRR19973083.2416076/1‑150 (MQ=60)
                                                AAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAG                                                                                                       >  SRR19973083.3702584/1‑150 (MQ=60)
                                                AAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAG                                                                                                       >  SRR19973083.3702577/1‑150 (MQ=60)
                                                    AGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCCGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGC                                                                                                   >  SRR19973083.2228254/1‑150 (MQ=60)
                                                    AGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGC                                                                                                   >  SRR19973083.374857/1‑150 (MQ=60)
                                                                 GATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTG                                                                                      >  SRR19973083.2103549/1‑150 (MQ=60)
                                                                 GATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTG                                                                                      >  SRR19973083.2830262/1‑150 (MQ=60)
                                                                   TACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCA                                                                                    >  SRR19973083.3479366/1‑150 (MQ=60)
                                                                     CCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAA                                                                                  >  SRR19973083.752318/1‑150 (MQ=60)
                                                                     CCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAA                                                                                  >  SRR19973083.3368997/1‑150 (MQ=60)
                                                                         CACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGA                                                                              <  SRR19973083.492624/150‑1 (MQ=60)
                                                                         CACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGA                                                                              <  SRR19973083.2559015/150‑1 (MQ=60)
                                                                         CACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGA                                                                              <  SRR19973083.492687/150‑1 (MQ=60)
                                                                          ACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGAC                                                                             >  SRR19973083.4895633/1‑150 (MQ=60)
                                                                             TCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGAT                                                                          <  SRR19973083.3017378/150‑1 (MQ=60)
                                                                             TCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGAT                                                                          <  SRR19973083.24017/150‑1 (MQ=60)
                                                                                TCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATTGCGGCAAAGGTATTAAAATCGATGAAGTTGTAAAAGGAAGCCCAGCTGCTCAGGCAGGCTTGCAAAAAGACGATGTG                                                                       >  SRR19973083.893127/1‑150 (MQ=60)
                                                                                  GTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGAT                                                                     >  SRR19973083.4708377/1‑150 (MQ=60)
                                                                                   TCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATC                                                                    <  SRR19973083.4271370/150‑1 (MQ=60)
                                                                                     GGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCAT                                                                  <  SRR19973083.1484961/150‑1 (MQ=60)
                                                                                     GGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCAT                                                                  <  SRR19973083.1484982/150‑1 (MQ=60)
                                                                                       CCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTG                                                                <  SRR19973083.1950343/150‑1 (MQ=60)
                                                                                       CCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTG                                                                <  SRR19973083.1950347/150‑1 (MQ=60)
                                                                                        CAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGG                                                               <  SRR19973083.3155512/150‑1 (MQ=60)
                                                                                        CAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGG                                                               <  SRR19973083.534947/150‑1 (MQ=60)
                                                                                        CAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGG                                                               >  SRR19973083.4333913/1‑150 (MQ=60)
                                                                                         AGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGC                                                              <  SRR19973083.3756244/150‑1 (MQ=60)
                                                                                           CGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGT                                                            <  SRR19973083.2323308/150‑1 (MQ=60)
                                                                                           CGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGT                                                            >  SRR19973083.1343213/1‑150 (MQ=60)
                                                                                            GCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTC                                                           >  SRR19973083.1300816/1‑150 (MQ=60)
                                                                                               GAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAAC                                                        <  SRR19973083.3295489/150‑1 (MQ=60)
                                                                                                AAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACC                                                       >  SRR19973083.1861734/1‑150 (MQ=60)
                                                                                                  ATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGC                                                     >  SRR19973083.1961832/1‑150 (MQ=60)
                                                                                                      TCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATC                                                 <  SRR19973083.4733708/150‑1 (MQ=60)
                                                                                                      TCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATC                                                 <  SRR19973083.4733439/150‑1 (MQ=60)
                                                                                                      TCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATC                                                 <  SRR19973083.11316/150‑1 (MQ=60)
                                                                                                            CAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGA                                           <  SRR19973083.1030608/150‑1 (MQ=60)
                                                                                                             AGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAA                                          >  SRR19973083.3851122/1‑150 (MQ=60)
                                                                                                                 CTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCG                                      >  SRR19973083.2262152/1‑150 (MQ=60)
                                                                                                                  TGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGA                                     >  SRR19973083.74865/1‑150 (MQ=60)
                                                                                                                    GAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATT                                   <  SRR19973083.2801386/150‑1 (MQ=60)
                                                                                                                    GAAGGTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATT                                   <  SRR19973083.2763211/150‑1 (MQ=60)
                                                                                                                    GAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATT                                   <  SRR19973083.4143243/150‑1 (MQ=60)
                                                                                                                        GTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTG                               <  SRR19973083.4070148/150‑1 (MQ=60)
                                                                                                                        GTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTG                               <  SRR19973083.206575/150‑1 (MQ=60)
                                                                                                                        GTGCAACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTG                               <  SRR19973083.4070160/150‑1 (MQ=60)
                                                                                                                            AACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAAT                           >  SRR19973083.4541214/1‑150 (MQ=60)
                                                                                                                            AACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAAT                           >  SRR19973083.4541030/1‑150 (MQ=60)
                                                                                                                            AACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAAT                           <  SRR19973083.2026613/150‑1 (MQ=60)
                                                                                                                            AACGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAAT                           <  SRR19973083.2026550/150‑1 (MQ=60)
                                                                                                                              CGTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGC                         <  SRR19973083.2058211/150‑1 (MQ=60)
                                                                                                                               GTTGAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCG                        >  SRR19973083.3521966/1‑150 (MQ=60)
                                                                                                                                  GAGCGATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAA                     <  SRR19973083.3732747/150‑1 (MQ=60)
                                                                                                                                      GATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTG                 >  SRR19973083.4617398/1‑150 (MQ=60)
                                                                                                                                      GATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTG                 >  SRR19973083.158059/1‑150 (MQ=60)
                                                                                                                                       ATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGC                >  SRR19973083.2573541/1‑150 (MQ=60)
                                                                                                                                       ATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGC                >  SRR19973083.1552895/1‑150 (MQ=60)
                                                                                                                                       ATGGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGC                >  SRR19973083.1553570/1‑150 (MQ=60)
                                                                                                                                         GGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTG              >  SRR19973083.395892/1‑150 (MQ=60)
                                                                                                                                         GGTCAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTG              >  SRR19973083.395894/1‑150 (MQ=60)
                                                                                                                                            CAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCG           <  SRR19973083.1421077/150‑1 (MQ=60)
                                                                                                                                            CAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCG           >  SRR19973083.4487322/1‑150 (MQ=60)
                                                                                                                                            CAGCTAAAATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCG           >  SRR19973083.4487312/1‑150 (MQ=60)
                                                                                                                                                  AAATATGGCGGCAAAGGTATTAAAATCGATAAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAA     >  SRR19973083.2977208/1‑150 (MQ=60)
                                                                                                                                                    ATATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACC   >  SRR19973083.1917049/1‑150 (MQ=60)
                                                                                                                                                    AGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACC   <  SRR19973083.3028204/150‑1 (MQ=60)
                                                                                                                                                     TATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCG  <  SRR19973083.1824828/150‑1 (MQ=60)
                                                                                                                                                     TATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCG  <  SRR19973083.3581688/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAGCGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGAAGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGAAATGCGTAAAGTGCTGGCGGCAAAACCG  >  CP009273/3375111‑3375409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑