Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,430,873 G→T 100% S93* (TCA→TAA)  ompN ← outer membrane pore protein N, non‑specific

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,430,8730GT95.3% 561.7 / 18.0 190S93* (TCA→TAA) ompNouter membrane pore protein N, non‑specific
Reads supporting (aligned to +/- strand):  ref base G (7/2);  new base T (93/88);  total (100/90)
Fisher's exact test for biased strand distribution p-value = 1.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 191 total aligned reads displayed.

GTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGAAA  >  CP009273/1430728‑1431020
                                                                                                                                                 |                                                                                                                                                   
  cTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTg                                                                                                                                                 <  2:1010510/148‑1 (MQ=255)
     cATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGtt                                                                                                                                              <  2:2286063/148‑1 (MQ=255)
      aTTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGttt                                                                                                                                             <  1:1254995/148‑1 (MQ=255)
       ttGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGtttt                                                                                                                                            <  1:2220037/148‑1 (MQ=255)
                gagTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGAtt                                                                                                                                   >  2:1676294/1‑148 (MQ=255)
                  gTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCa                                                                                                                                 <  1:3207146/148‑1 (MQ=255)
                  gTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCa                                                                                                                                 <  1:140041/148‑1 (MQ=255)
                    cACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAgt                                                                                                                               >  2:1271836/1‑148 (MQ=255)
                     aCCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAgtg                                                                                                                              <  2:2481668/148‑1 (MQ=255)
                     aCCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAgtg                                                                                                                              <  2:1542966/148‑1 (MQ=255)
                      ccGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAgtgt                                                                                                                             <  2:2265475/148‑1 (MQ=255)
                      ccGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAgtgt                                                                                                                             >  1:204563/1‑148 (MQ=255)
                      ccGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAgtgt                                                                                                                             >  1:1656095/1‑148 (MQ=255)
                      ccGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAgtgt                                                                                                                             >  2:2656828/1‑148 (MQ=255)
                      ccGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGAGGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAgtgt                                                                                                                             <  2:1359342/148‑1 (MQ=255)
                       cGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGtgtt                                                                                                                            >  2:782275/1‑148 (MQ=255)
                          cAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTgttgtt                                                                                                                         <  1:1464944/148‑1 (MQ=255)
                          cAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTgttgtt                                                                                                                         <  1:1433813/148‑1 (MQ=255)
                           aaacacAGGCAGCAGCTCGGTCCAGCCGACGAGCGCGTACAATACGCCAGAAGTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGttt                                                                                                                        <  2:2205756/143‑1 (MQ=255)
                           aaaTTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGttt                                                                                                                        <  2:2205693/148‑1 (MQ=255)
                             aTTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGc                                                                                                                      >  2:971501/1‑148 (MQ=255)
                              ttCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGcc                                                                                                                     >  2:2988564/1‑148 (MQ=255)
                               tCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCt                                                                                                                    >  1:1320231/1‑148 (MQ=255)
                               tCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCt                                                                                                                    >  1:2968067/1‑148 (MQ=255)
                                   gcagcaTATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAat                                                                                                                <  2:1945464/148‑1 (MQ=255)
                                       caTATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTg                                                                                                            >  1:3198955/1‑148 (MQ=255)
                                        atatCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGt                                                                                                           <  2:803929/148‑1 (MQ=255)
                                         tatCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTa                                                                                                          <  1:3284460/148‑1 (MQ=255)
                                         tatCGGTCAACCCTTCGATTTCGTACATTACGCCATAATTAAGTCAGTAATCGCAAGAACCGTAATCTGCAAATTTCAGCCAGGCAAAGTCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGACTGTCAATTGTa                                                                                                          >  2:1699486/1‑148 (MQ=255)
                                          atCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTAt                                                                                                         <  1:2142965/148‑1 (MQ=255)
                                          atCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTAt                                                                                                         >  1:2007016/1‑148 (MQ=255)
                                          atCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTAt                                                                                                         <  2:31559/148‑1 (MQ=255)
                                             ggTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTcc                                                                                                      <  1:162427/148‑1 (MQ=255)
                                             ggTCCAGCCTTCGATGTAGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTcc                                                                                                      <  1:1643318/148‑1 (MQ=255)
                                               tCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCa                                                                                                    <  2:2131677/148‑1 (MQ=255)
                                                ccAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCAt                                                                                                   >  2:2042122/1‑148 (MQ=255)
                                                    ccTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAc                                                                                               >  2:2387611/1‑148 (MQ=255)
                                                    ccTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAc                                                                                               >  1:39153/1‑148 (MQ=255)
                                                    ccTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAc                                                                                               >  1:2696970/1‑148 (MQ=255)
                                                         gATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAg                                                                                          <  2:403201/148‑1 (MQ=255)
                                                          aTGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGc                                                                                         >  2:3037418/1‑148 (MQ=255)
                                                           tGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGcc                                                                                        <  1:2795563/148‑1 (MQ=255)
                                                            gTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCa                                                                                       >  2:503699/1‑148 (MQ=255)
                                                              cGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGt                                                                                     >  2:185046/1‑148 (MQ=255)
                                                               gTACATTACGACATAATTACGTCCGTAATCGAAATAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTg                                                                                    <  2:1938854/148‑1 (MQ=255)
                                                                    ttACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTg                                                                               >  2:858007/1‑148 (MQ=255)
                                                                     tACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGa                                                                              <  2:949315/148‑1 (MQ=255)
                                                                        gCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCg                                                                           <  2:2935938/148‑1 (MQ=255)
                                                                        gCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCg                                                                           <  2:3198955/148‑1 (MQ=255)
                                                                          cATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGtt                                                                         >  1:1369568/1‑148 (MQ=255)
                                                                          cATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGtt                                                                         >  1:2441705/1‑148 (MQ=255)
                                                                            tAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGTCAGTGAGTTGATCGTTaa                                                                       <  1:537371/148‑1 (MQ=255)
                                                                            tAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTaa                                                                       >  2:1677832/1‑148 (MQ=255)
                                                                             aaTTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAt                                                                      >  2:1117720/1‑148 (MQ=255)
                                                                             aaTTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAt                                                                      <  2:702745/148‑1 (MQ=255)
                                                                             aaTTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAt                                                                      <  1:2781931/148‑1 (MQ=255)
                                                                               ttACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAttt                                                                    >  2:3095112/1‑148 (MQ=255)
                                                                               ttACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAttt                                                                    <  1:2891044/148‑1 (MQ=255)
                                                                                    tCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGtt                                                               >  2:2523544/1‑148 (MQ=255)
                                                                                     ccGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGttt                                                              >  1:2351375/1‑148 (MQ=255)
                                                                                     ccGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGttt                                                              <  1:3105379/148‑1 (MQ=255)
                                                                                        tAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGc                                                           <  1:3153978/148‑1 (MQ=255)
                                                                                         aaTCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGcc                                                          <  1:2258163/148‑1 (MQ=255)
                                                                                           tCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCtt                                                        >  1:765587/1‑148 (MQ=255)
                                                                                           tCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCtt                                                        <  2:945430/148‑1 (MQ=255)
                                                                                                aGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTaaaa                                                   >  1:1651780/1‑148 (MQ=255)
                                                                                                aGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTaaaa                                                   >  2:2539585/1‑148 (MQ=255)
                                                                                                  aaCCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAAcc                                                 <  2:39153/148‑1 (MQ=255)
                                                                                                  aaCCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAAcc                                                 <  2:2696888/148‑1 (MQ=255)
                                                                                                  aaCCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAAcc                                                 >  2:122671/1‑148 (MQ=255)
                                                                                                    ccGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCa                                               >  2:893121/1‑148 (MQ=255)
                                                                                                    ccGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCa                                               >  2:1373441/1‑148 (MQ=255)
                                                                                                     cGTAATCTGCAAATTTCAGCCCGGCAAATGACAGACGGGTCCATTAATTGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCTCCTTTAAAACCCAg                                              >  2:3123953/1‑148 (MQ=255)
                                                                                                      gTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGa                                             >  1:716420/1‑148 (MQ=255)
                                                                                                          tCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGAcgcg                                         >  2:1063735/1‑148 (MQ=255)
                                                                                                           cttcAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACgcgc                                        <  2:2884243/145‑1 (MQ=255)
                                                                                                           cTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACgcgc                                        <  2:2885056/148‑1 (MQ=255)
                                                                                                           cTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACccgc                                        >  1:1253761/1‑148 (MQ=255)
                                                                                                            tGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCa                                       >  1:2603811/1‑148 (MQ=255)
                                                                                                             gCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCAt                                      >  1:1871082/1‑148 (MQ=255)
                                                                                                               aaaTTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAg                                    <  2:2060210/148‑1 (MQ=255)
                                                                                                               aaaTTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAg                                    <  2:1656095/148‑1 (MQ=255)
                                                                                                                aaTTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGc                                   >  2:1638392/1‑148 (MQ=255)
                                                                                                                aaTTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGc                                   <  1:955707/148‑1 (MQ=255)
                                                                                                                aaTTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAAACCAGACGCGCATAGc                                   >  2:1752739/1‑148 (MQ=255)
                                                                                                                 aTTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGct                                  <  1:896739/148‑1 (MQ=255)
                                                                                                                           ggCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCa                        >  2:1144323/1‑148 (MQ=255)
                                                                                                                               aaTGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACTCAGACGCGCATAGCTCTGGTCGCCATCtt                    <  2:3014273/148‑1 (MQ=255)
                                                                                                                               aaTGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCtt                    <  1:1106687/148‑1 (MQ=255)
                                                                                                                                ctGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCttt                   <  1:2540383/147‑1 (MQ=255)
                                                                                                                                aTGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCttt                   >  1:2820519/1‑148 (MQ=255)
                                                                                                                                aTGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCttt                   <  1:2276671/148‑1 (MQ=255)
                                                                                                                                  gCCAGACGGGTCCATTACTGGTTTTTTGAATATTCAGTGTTGTTTTCATTAATAATTTATTCCCAATGAACGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAAACCAGACTCCCAAAGCTCTGGTCGCCATCtttat                 >  1:9065/1‑146 (MQ=255)
                                                                                                                                   ccAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGc                >  1:478972/1‑148 (MQ=255)
                                                                                                                                     aGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTg              <  2:2858808/148‑1 (MQ=255)
                                                                                                                                       aCGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGaa            >  2:2938149/1‑148 (MQ=255)
                                                                                                                                       aCGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGaa            >  1:1429854/1‑148 (MQ=255)
                                                                                                                                        cGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAAt           >  2:669274/1‑148 (MQ=255)
                                                                                                                                             ccATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCa      >  1:2190760/1‑148 (MQ=255)
                                                                                                                                                tGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAAACCAGACGCGCATAGCTATGGTCGCCATCTTTCGCTGAATTATAAGaa   >  1:153867/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGAAA  >  CP009273/1430728‑1431020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGAAAA  >  CP009273/1430731‑1431021
                                                                                                                                              |                                                                                                                                                    
TGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTT                                                                                                                                               >  SRR19973070.1350209/1‑150 (MQ=60)
   ATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTT                                                                                                                                            <  SRR19973070.1263222/150‑1 (MQ=60)
   ATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTT                                                                                                                                            <  SRR19973070.1263798/150‑1 (MQ=60)
    TTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTT                                                                                                                                           <  SRR19973070.2234584/150‑1 (MQ=60)
    TTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTT                                                                                                                                           <  SRR19973070.2234614/150‑1 (MQ=60)
     TGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTG                                                                                                                                          >  SRR19973070.2302570/1‑150 (MQ=60)
      GGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGA                                                                                                                                         <  SRR19973070.3129884/150‑1 (MQ=60)
           AAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATT                                                                                                                                    >  SRR19973070.908809/1‑150 (MQ=60)
               GTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGT                                                                                                                                <  SRR19973070.3227112/150‑1 (MQ=60)
               GTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGT                                                                                                                                <  SRR19973070.3227889/150‑1 (MQ=60)
               GTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGT                                                                                                                                <  SRR19973070.141261/150‑1 (MQ=60)
                 CACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGT                                                                                                                              >  SRR19973070.1667507/1‑150 (MQ=60)
                 CACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGT                                                                                                                              >  SRR19973070.1667494/1‑150 (MQ=60)
                 CACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGT                                                                                                                              >  SRR19973070.206101/1‑150 (MQ=60)
                       CAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTG                                                                                                                        <  SRR19973070.1443727/150‑1 (MQ=60)
                       CAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTG                                                                                                                        <  SRR19973070.1443848/150‑1 (MQ=60)
                       CAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTG                                                                                                                        <  SRR19973070.1475179/150‑1 (MQ=60)
                          ATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCT                                                                                                                     >  SRR19973070.2987139/1‑150 (MQ=60)
                          ATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCT                                                                                                                     <  SRR19973070.531478/150‑1 (MQ=60)
                          ATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCT                                                                                                                     >  SRR19973070.1329364/1‑150 (MQ=60)
                            TCCGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGA                                                                                                                   <  SRR19973070.1419184/150‑1 (MQ=60)
                                  AGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTG                                                                                                             >  SRR19973070.3219657/1‑150 (MQ=60)
                                     ATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTAT                                                                                                          >  SRR19973070.2020186/1‑150 (MQ=60)
                                     ATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTAT                                                                                                          >  SRR19973070.2020607/1‑150 (MQ=60)
                                      TATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATT                                                                                                         <  SRR19973070.3305549/150‑1 (MQ=60)
                                       ATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTC                                                                                                        <  SRR19973070.2157255/150‑1 (MQ=60)
                                       ATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTC                                                                                                        <  SRR19973070.224143/150‑1 (MQ=60)
                                          GGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCA                                                                                                     <  SRR19973070.163759/150‑1 (MQ=60)
                                          GGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCA                                                                                                     <  SRR19973070.1653938/150‑1 (MQ=60)
                                          GGTCCAGCCTTCGATGTAGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCA                                                                                                     <  SRR19973070.1654677/150‑1 (MQ=60)
                                               AGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAC                                                                                                >  SRR19973070.39654/1‑150 (MQ=60)
                                               AGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAC                                                                                                >  SRR19973070.2714347/1‑150 (MQ=60)
                                               AGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAC                                                                                                >  SRR19973070.2714429/1‑150 (MQ=60)
                                                 CCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCG                                                                                              <  SRR19973070.1381994/150‑1 (MQ=60)
                                                        TGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAG                                                                                       <  SRR19973070.2813599/150‑1 (MQ=60)
                                                         GTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGT                                                                                      >  SRR19973070.94849/1‑150 (MQ=60)
                                                         GTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGT                                                                                      >  SRR19973070.2528888/1‑150 (MQ=60)
                                                           CGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGA                                                                                    <  SRR19973070.2806433/150‑1 (MQ=60)
                                                                ATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGA                                                                               >  SRR19973070.3096298/1‑150 (MQ=60)
                                                                    CGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGT                                                                           >  SRR19973070.2284277/1‑150 (MQ=60)
                                                                     GCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTT                                                                          <  SRR19973070.3293748/150‑1 (MQ=60)
                                                                     GCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTT                                                                          >  SRR19973070.1378886/1‑150 (MQ=60)
                                                                     GCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTT                                                                          <  SRR19973070.2093242/150‑1 (MQ=60)
                                                                     GCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTT                                                                          >  SRR19973070.2457702/1‑150 (MQ=60)
                                                                        ATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCACATTGACCGTATCAAGTGAGTTGATCTTTAAT                                                                       >  SRR19973070.1569052/1‑150 (MQ=60)
                                                                         TAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGTCAGTGAGTTGATCGTTAATT                                                                      <  SRR19973070.540972/150‑1 (MQ=60)
                                                                          AATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTT                                                                     <  SRR19973070.2799815/150‑1 (MQ=60)
                                                                            TTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGG                                                                   <  SRR19973070.2909755/150‑1 (MQ=60)
                                                                                GTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTT                                                               >  SRR19973070.2366960/1‑150 (MQ=60)
                                                                                  CCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCG                                                             <  SRR19973070.3125410/150‑1 (MQ=60)
                                                                                  CCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCG                                                             <  SRR19973070.874531/150‑1 (MQ=60)
                                                                                     TAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCT                                                          <  SRR19973070.129280/150‑1 (MQ=60)
                                                                                     TAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCT                                                          <  SRR19973070.3174357/150‑1 (MQ=60)
                                                                                      AATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTT                                                         >  SRR19973070.770966/1‑150 (MQ=60)
                                                                                      AATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTT                                                         <  SRR19973070.2273138/150‑1 (MQ=60)
                                                                                         CGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAA                                                      >  SRR19973070.1368512/1‑150 (MQ=60)
                                                                                         CGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTACCATTGACCGTAGCCAGTGAGATGATCGTTAATTTGGGTTTCGCCTTTAA                                                      >  SRR19973070.2877333/1‑150 (MQ=60)
                                                                                           AAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAA                                                    >  SRR19973070.1663175/1‑150 (MQ=60)
                                                                                            tggagttcagacgtgtgctcttccgatctGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAAC                                                   <  SRR19973070.74436/121‑1 (MQ=60)
                                                                                            AAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAAC                                                   <  SRR19973070.1539165/150‑1 (MQ=60)
                                                                                               AACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCA                                                >  SRR19973070.2058764/1‑150 (MQ=60)
                                                                                                 CCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGA                                              >  SRR19973070.721530/1‑150 (MQ=60)
                                                                                                      ATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGC                                         >  SRR19973070.2921716/1‑150 (MQ=60)
                                                                                                      ATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACCCGC                                         >  SRR19973070.1262553/1‑150 (MQ=60)
                                                                                                       TCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCA                                        >  SRR19973070.2620811/1‑150 (MQ=60)
                                                                                                       TCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCA                                        >  SRR19973070.2620808/1‑150 (MQ=60)
                                                                                                        CTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCAT                                       >  SRR19973070.1883846/1‑150 (MQ=60)
                                                                                                             AATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTC                                  <  SRR19973070.962528/150‑1 (MQ=60)
                                                                                                              ATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCT                                 <  SRR19973070.903087/150‑1 (MQ=60)
                                                                                                              ATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCT                                 <  SRR19973070.553400/150‑1 (MQ=60)
                                                                                                                   AGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCG                            <  SRR19973070.2836518/150‑1 (MQ=60)
                                                                                                                   AGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCG                            <  SRR19973070.400473/150‑1 (MQ=60)
                                                                                                                           AAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTT                    >  SRR19973070.2838755/1‑150 (MQ=60)
                                                                                                                           AAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTT                    >  SRR19973070.1710433/1‑150 (MQ=60)
                                                                                                                            AATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTC                   <  SRR19973070.1114269/150‑1 (MQ=60)
                                                                                                                             CTGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCG                  <  SRR19973070.2556995/150‑1 (MQ=60)
                                                                                                                             ATGCCAGACGGGTCCATTACTGGTTTTTTGAATATTCAGTGTTGTTTTCATTAATAATTTATTCCCAATGAACGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAAACCAGACTCCCAAAGCTCTGGTCGCCATCTTTat                  >  SRR19973070.9200/1‑148 (MQ=60)
                                                                                                                             ATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCG                  <  SRR19973070.3330790/150‑1 (MQ=60)
                                                                                                                             ATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCG                  <  SRR19973070.2291793/150‑1 (MQ=60)
                                                                                                                              TGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGC                 >  SRR19973070.2158017/1‑150 (MQ=60)
                                                                                                                              TGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGC                 >  SRR19973070.482353/1‑150 (MQ=60)
                                                                                                                              TGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGC                 >  SRR19973070.2840663/1‑150 (MQ=60)
                                                                                                                               GCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCT                >  SRR19973070.2720153/1‑150 (MQ=60)
                                                                                                                                  AGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAA             >  SRR19973070.1439724/1‑150 (MQ=60)
                                                                                                                                  AGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCACTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAA             >  SRR19973070.1439793/1‑150 (MQ=60)
                                                                                                                                        GTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCA       >  SRR19973070.2205226/1‑150 (MQ=60)
                                                                                                                                           CATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAAACCAGACGCGCATAGCTATGGTCGCCATCTTTCGCTGAATTATAAGAA    >  SRR19973070.155152/1‑150 (MQ=60)
                                                                                                                                             TTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGAAAA  >  SRR19973070.568408/1‑150 (MQ=60)
                                                                                                                                             TTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGAAAA  >  SRR19973070.516942/1‑150 (MQ=60)
                                                                                                                                             TTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGAAAA  >  SRR19973070.1540540/1‑150 (MQ=60)
                                                                                                                                              |                                                                                                                                                    
TGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGAAAA  >  CP009273/1430731‑1431021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑