Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,430,873 G→T 100% S93* (TCA→TAA)  ompN ← outer membrane pore protein N, non‑specific

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,430,8730GT96.8% 762.0 / 14.2 249S93* (TCA→TAA) ompNouter membrane pore protein N, non‑specific
Reads supporting (aligned to +/- strand):  ref base G (1/7);  new base T (127/114);  total (128/121)
Fisher's exact test for biased strand distribution p-value = 3.18e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 249 total aligned reads displayed.

GTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAG  >  CP009273/1430728‑1431017
                                                                                                                                                 |                                                                                                                                                
gTCTGCATTGGTATAAGAGTCACCGCCTAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTAc                                                                                                                                                <  1:2322080/148‑1 (MQ=255)
 tCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACt                                                                                                                                               <  1:969482/148‑1 (MQ=255)
 tCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACt                                                                                                                                               >  1:1689381/1‑148 (MQ=255)
   tGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTgg                                                                                                                                             <  2:67210/148‑1 (MQ=255)
    gCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGt                                                                                                                                            >  1:2192230/1‑148 (MQ=255)
    gCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGt                                                                                                                                            <  2:698423/148‑1 (MQ=255)
    gCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGt                                                                                                                                            <  2:2460350/148‑1 (MQ=255)
     cATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGtt                                                                                                                                           <  1:3132113/148‑1 (MQ=255)
      aTTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGttt                                                                                                                                          <  1:2385208/148‑1 (MQ=255)
       ttGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGtttt                                                                                                                                         >  1:692888/1‑148 (MQ=255)
        tGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGGTGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGACAAATGCCAGACGGGTCCATTACTGGttttt                                                                                                                                        >  1:1151933/1‑148 (MQ=255)
             taAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAg                                                                                                                                   <  1:1871618/148‑1 (MQ=255)
              aaGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGa                                                                                                                                  >  1:579709/1‑148 (MQ=255)
              aaGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGa                                                                                                                                  <  2:2288472/148‑1 (MQ=255)
               agagTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGAt                                                                                                                                 <  2:339050/148‑1 (MQ=255)
               agagTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGAt                                                                                                                                 <  1:2954623/148‑1 (MQ=255)
                      ccGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAgtgt                                                                                                                          >  2:466011/1‑148 (MQ=255)
                        gCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGtgttg                                                                                                                        >  1:2652076/1‑148 (MQ=255)
                         ccAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGtgttgt                                                                                                                       >  1:125089/1‑148 (MQ=255)
                          cAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTgttgtt                                                                                                                      >  1:1215762/1‑148 (MQ=255)
                          cAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTgttgtt                                                                                                                      >  1:948010/1‑148 (MQ=255)
                            aaTTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTg                                                                                                                    <  1:2469755/148‑1 (MQ=255)
                             aTTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGc                                                                                                                   <  2:3024711/148‑1 (MQ=255)
                              ttCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGcc                                                                                                                  <  1:1235387/148‑1 (MQ=255)
                               tCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCt                                                                                                                 <  1:2034585/148‑1 (MQ=255)
                               tCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCt                                                                                                                 <  1:809871/148‑1 (MQ=255)
                                        atatCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGt                                                                                                        >  1:1548152/1‑148 (MQ=255)
                                          atCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTAt                                                                                                      <  2:2717061/148‑1 (MQ=255)
                                           tCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTAtt                                                                                                     <  1:2671971/148‑1 (MQ=255)
                                               tCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCa                                                                                                 <  2:687144/148‑1 (MQ=255)
                                               tCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCa                                                                                                 >  1:1601541/1‑148 (MQ=255)
                                                  aGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTg                                                                                              >  2:1825895/1‑148 (MQ=255)
                                                    ccTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAc                                                                                            >  1:765775/1‑148 (MQ=255)
                                                      ttCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCg                                                                                          <  1:1486631/148‑1 (MQ=255)
                                                          aTGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGc                                                                                      >  2:1767219/1‑148 (MQ=255)
                                                          aTGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGc                                                                                      <  1:3297423/148‑1 (MQ=255)
                                                          aTGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGc                                                                                      >  2:3503857/1‑148 (MQ=255)
                                                           tGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGcc                                                                                     <  2:2960857/148‑1 (MQ=255)
                                                            gTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCa                                                                                    <  1:1007666/148‑1 (MQ=255)
                                                             tCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAg                                                                                   >  2:1488879/1‑148 (MQ=255)
                                                                 aCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAg                                                                               <  2:281164/148‑1 (MQ=255)
                                                                 aCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAg                                                                               >  2:3335322/1‑148 (MQ=255)
                                                                 aCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAg                                                                               <  2:328892/148‑1 (MQ=255)
                                                                     tACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGa                                                                           <  2:2766257/148‑1 (MQ=255)
                                                                      aCGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGAt                                                                          <  2:2064911/148‑1 (MQ=255)
                                                                            tAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTaa                                                                    >  2:897285/1‑148 (MQ=255)
                                                                             aaTTACGTCCGTACTCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAt                                                                   <  2:3115914/148‑1 (MQ=255)
                                                                             aaTTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAt                                                                   <  2:2564294/148‑1 (MQ=255)
                                                                             aaTTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAt                                                                   <  2:3117511/148‑1 (MQ=255)
                                                                               ttACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAttt                                                                 >  2:1956610/1‑148 (MQ=255)
                                                                                tACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTg                                                                >  2:3427800/1‑148 (MQ=255)
                                                                                tACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTg                                                                >  1:1699272/1‑148 (MQ=255)
                                                                                  cGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTggg                                                              >  1:1135482/1‑148 (MQ=255)
                                                                                    tCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGtt                                                            <  2:1530262/148‑1 (MQ=255)
                                                                                     ccGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGttt                                                           >  1:3206874/1‑148 (MQ=255)
                                                                                     ccGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGttt                                                           <  2:1548152/148‑1 (MQ=255)
                                                                                       gTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCg                                                         <  2:2736631/148‑1 (MQ=255)
                                                                                        tAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGc                                                        <  1:1974088/148‑1 (MQ=255)
                                                                                        tAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGc                                                        <  1:2780427/148‑1 (MQ=255)
                                                                                         aaTCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGcc                                                       >  1:1341993/1‑148 (MQ=255)
                                                                                           tCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCtt                                                     >  2:2167660/1‑148 (MQ=255)
                                                                                           tCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCtt                                                     >  2:3389718/1‑148 (MQ=255)
                                                                                            cGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCttt                                                    >  2:1449139/1‑148 (MQ=255)
                                                                                            cGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCttt                                                    >  2:2639864/1‑148 (MQ=255)
                                                                                               aaGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTaaa                                                 >  2:734847/1‑148 (MQ=255)
                                                                                               aaGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTaaa                                                 <  1:644878/148‑1 (MQ=255)
                                                                                               aaGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTaaa                                                 >  1:551403/1‑148 (MQ=255)
                                                                                               aaGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTaaa                                                 <  1:3376069/148‑1 (MQ=255)
                                                                                                aGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGt                                                             <  2:1136650/135‑1 (MQ=255)
                                                                                                aGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTaaaa                                                <  1:1247213/148‑1 (MQ=255)
                                                                                                    ccGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCa                                            >  2:1658610/1‑148 (MQ=255)
                                                                                                    ccGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCa                                            >  2:1645170/1‑148 (MQ=255)
                                                                                                     cGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAg                                           <  2:2487791/148‑1 (MQ=255)
                                                                                                     cGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAg                                           <  2:406623/148‑1 (MQ=255)
                                                                                                       tAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGAc                                         >  2:2381047/1‑148 (MQ=255)
                                                                                                           cTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACgcgc                                     >  2:2144465/1‑148 (MQ=255)
                                                                                                            tGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCa                                    >  1:2184314/1‑148 (MQ=255)
                                                                                                             gCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCAt                                   >  2:1248190/1‑148 (MQ=255)
                                                                                                             gCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCAt                                   >  1:2481731/1‑148 (MQ=255)
                                                                                                             gCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCAt                                   >  1:2733786/1‑148 (MQ=255)
                                                                                                                 aTTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGct                               <  1:1899325/148‑1 (MQ=255)
                                                                                                                 aTTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGct                               <  1:2699461/148‑1 (MQ=255)
                                                                                                                  tttCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGctc                              >  2:746050/1‑148 (MQ=255)
                                                                                                                      aGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGt                          <  2:765936/148‑1 (MQ=255)
                                                                                                                       gCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTc                         >  2:2572716/1‑148 (MQ=255)
                                                                                                                       gCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTc                         >  2:2478334/1‑148 (MQ=255)
                                                                                                                        cccGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCg                        <  1:1001400/148‑1 (MQ=255)
                                                                                                                        cccGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCg                        <  1:2755587/148‑1 (MQ=255)
                                                                                                                            gCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCAt                    >  1:2730873/1‑148 (MQ=255)
                                                                                                                                aTGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCttt                >  2:945317/1‑148 (MQ=255)
                                                                                                                                aTGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCttt                >  2:2508280/1‑148 (MQ=255)
                                                                                                                                aTGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCttt                >  2:1203636/1‑148 (MQ=255)
                                                                                                                                 tGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTAGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTc               >  1:108310/1‑148 (MQ=255)
                                                                                                                                    cAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCt            >  1:2082821/1‑148 (MQ=255)
                                                                                                                                      gACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGa          <  1:3176660/148‑1 (MQ=255)
                                                                                                                                       aCGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGaa         <  2:185015/148‑1 (MQ=255)
                                                                                                                                       aCGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGaa         <  1:2542715/148‑1 (MQ=255)
                                                                                                                                            tCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATc    <  1:1825895/148‑1 (MQ=255)
                                                                                                                                             ccATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCa   >  2:329876/1‑148 (MQ=255)
                                                                                                                                             ccATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCa   >  2:328354/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAG  >  CP009273/1430728‑1431017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGAAAAA  >  CP009273/1430727‑1431022
                                                                                                                                                  |                                                                                                                                                     
TGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACT                                                                                                                                                    >  SRR19973065.1700661/1‑150 (MQ=60)
TGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTCCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACT                                                                                                                                                    <  SRR19973065.1834376/150‑1 (MQ=60)
 GTCTGCATTGGTATAAGAGTCACCGCCTAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTG                                                                                                                                                   <  SRR19973065.2336629/150‑1 (MQ=60)
 GTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGAAATCTGCAAATATCAGCCCGTCAAATGCCAGACGGGTCCATTACTG                                                                                                                                                   >  SRR19973065.400350/1‑150 (MQ=60)
  TCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGG                                                                                                                                                  <  SRR19973065.975918/150‑1 (MQ=60)
   CTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGT                                                                                                                                                 >  SRR19973065.2206286/1‑150 (MQ=60)
     GCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTT                                                                                                                                               <  SRR19973065.1559214/150‑1 (MQ=60)
      CATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTT                                                                                                                                              <  SRR19973065.3151165/150‑1 (MQ=60)
      CATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTT                                                                                                                                              >  SRR19973065.2470389/1‑150 (MQ=60)
      CATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTT                                                                                                                                              >  SRR19973065.2078072/1‑150 (MQ=60)
      CATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTT                                                                                                                                              >  SRR19973065.697502/1‑150 (MQ=60)
       ATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGGTGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGACAAATGCCAGACGGGTCCATTACTGGTTTTT                                                                                                                                             >  SRR19973065.1159347/1‑150 (MQ=60)
       ATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTT                                                                                                                                             <  SRR19973065.2400167/150‑1 (MQ=60)
       ATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGACAAATGCCAGACGGGTCCATTACTGGTTTTT                                                                                                                                             >  SRR19973065.1159945/1‑150 (MQ=60)
         TGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTG                                                                                                                                           <  SRR19973065.2347918/150‑1 (MQ=60)
         TGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTG                                                                                                                                           <  SRR19973065.2796312/150‑1 (MQ=60)
             ATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGA                                                                                                                                       >  SRR19973065.583336/1‑150 (MQ=60)
             ATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGA                                                                                                                                       >  SRR19973065.582447/1‑150 (MQ=60)
              TAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGAT                                                                                                                                      <  SRR19973065.1883944/150‑1 (MQ=60)
               AAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAATATT                                                                                                                                     >  SRR19973065.638507/1‑150 (MQ=60)
                AGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTC                                                                                                                                    >  SRR19973065.3252245/1‑150 (MQ=60)
                AGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTC                                                                                                                                    <  SRR19973065.2972645/150‑1 (MQ=60)
                AGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTC                                                                                                                                    >  SRR19973065.1519991/1‑150 (MQ=60)
                   GTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGT                                                                                                                                 <  SRR19973065.89506/150‑1 (MQ=60)
                       CCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTG                                                                                                                             >  SRR19973065.2668442/1‑150 (MQ=60)
                       CCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTG                                                                                                                             <  SRR19973065.1830628/150‑1 (MQ=60)
                        CGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGT                                                                                                                            >  SRR19973065.126227/1‑150 (MQ=60)
                        CGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGT                                                                                                                            >  SRR19973065.126172/1‑150 (MQ=60)
                         GCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTT                                                                                                                           >  SRR19973065.954272/1‑150 (MQ=60)
                         GCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTT                                                                                                                           >  SRR19973065.1225663/1‑150 (MQ=60)
                         GCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTT                                                                                                                           >  SRR19973065.1223612/1‑150 (MQ=60)
                         GCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTT                                                                                                                           >  SRR19973065.1223645/1‑150 (MQ=60)
                           CAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTG                                                                                                                         >  SRR19973065.1884880/1‑150 (MQ=60)
                            AAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGC                                                                                                                        >  SRR19973065.1255431/1‑150 (MQ=60)
                            AAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGC                                                                                                                        >  SRR19973065.1255423/1‑150 (MQ=60)
                            AAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGAAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGC                                                                                                                        >  SRR19973065.1255394/1‑150 (MQ=60)
                             AATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCC                                                                                                                       <  SRR19973065.2485391/150‑1 (MQ=60)
                             AATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCC                                                                                                                       <  SRR19973065.2485310/150‑1 (MQ=60)
                              ATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCT                                                                                                                      <  SRR19973065.2901736/150‑1 (MQ=60)
                              ATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCT                                                                                                                      <  SRR19973065.2185567/150‑1 (MQ=60)
                               TTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTG                                                                                                                     <  SRR19973065.1243483/150‑1 (MQ=60)
                                TCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGA                                                                                                                    <  SRR19973065.2047631/150‑1 (MQ=60)
                                TCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGA                                                                                                                    <  SRR19973065.815217/150‑1 (MQ=60)
                                    GCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATAT                                                                                                                >  SRR19973065.2753653/1‑150 (MQ=60)
                                     CAGCGTATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATT                                                                                                               <  SRR19973065.1054562/150‑1 (MQ=60)
                                       GCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGT                                                                                                             >  SRR19973065.1558397/1‑150 (MQ=60)
                                       GCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGT                                                                                                             <  SRR19973065.2724256/150‑1 (MQ=60)
                                            TCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCC                                                                                                        <  SRR19973065.2688489/150‑1 (MQ=60)
                                            TCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCC                                                                                                        <  SRR19973065.2688554/150‑1 (MQ=60)
                                            TCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCC                                                                                                        <  SRR19973065.2688527/150‑1 (MQ=60)
                                              GGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCA                                                                                                      >  SRR19973065.1612245/1‑150 (MQ=60)
                                              GGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCA                                                                                                      <  SRR19973065.2185427/150‑1 (MQ=60)
                                                   AGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAC                                                                                                 >  SRR19973065.88921/1‑150 (MQ=60)
                                                   AGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAC                                                                                                 >  SRR19973065.770865/1‑150 (MQ=60)
                                                   AGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAC                                                                                                 >  SRR19973065.771026/1‑150 (MQ=60)
                                                    GCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACC                                                                                                <  SRR19973065.1635477/150‑1 (MQ=60)
                                                       TTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTA                                                                                             <  SRR19973065.1496128/150‑1 (MQ=60)
                                                        TCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAG                                                                                            <  SRR19973065.1851994/150‑1 (MQ=60)
                                                         CGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGC                                                                                           >  SRR19973065.1300081/1‑150 (MQ=60)
                                                          GATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTAAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCC                                                                                          >  SRR19973065.1091150/1‑150 (MQ=60)
                                                           ATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCA                                                                                         <  SRR19973065.3317505/150‑1 (MQ=60)
                                                             GTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGT                                                                                       <  SRR19973065.1014352/150‑1 (MQ=60)
                                                                  ACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTT                                                                                  <  SRR19973065.610239/150‑1 (MQ=60)
                                                                  ACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTT                                                                                  <  SRR19973065.1531531/150‑1 (MQ=60)
                                                                    ATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGA                                                                                >  SRR19973065.2530437/1‑150 (MQ=60)
                                                                    ATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGA                                                                                >  SRR19973065.2530443/1‑150 (MQ=60)
                                                                      TACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATC                                                                              >  SRR19973065.649557/1‑150 (MQ=60)
                                                                        CGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGT                                                                            >  SRR19973065.1706566/1‑150 (MQ=60)
                                                                        CGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGT                                                                            <  SRR19973065.3158609/150‑1 (MQ=60)
                                                                          CCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTA                                                                          >  SRR19973065.65133/1‑150 (MQ=60)
                                                                               ATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTG                                                                     >  SRR19973065.1710602/1‑150 (MQ=60)
                                                                                 TACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGG                                                                   >  SRR19973065.1142843/1‑150 (MQ=60)
                                                                                    GTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTT                                                                >  SRR19973065.3226176/1‑150 (MQ=60)
                                                                                     TCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTC                                                               >  SRR19973065.2680132/1‑150 (MQ=60)
                                                                                       CGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGC                                                             >  SRR19973065.2626707/1‑150 (MQ=60)
                                                                                        GTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCC                                                            <  SRR19973065.1097766/150‑1 (MQ=60)
                                                                                        GTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCC                                                            >  SRR19973065.1350871/1‑150 (MQ=60)
                                                                                         TAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCT                                                           <  SRR19973065.2797679/150‑1 (MQ=60)
                                                                                         TAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCT                                                           <  SRR19973065.1986946/150‑1 (MQ=60)
                                                                                         TAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCT                                                           <  SRR19973065.1986941/150‑1 (MQ=60)
                                                                                             CGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAA                                                       >  SRR19973065.2023290/1‑150 (MQ=60)
                                                                                              GAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAA                                                      >  SRR19973065.554945/1‑150 (MQ=60)
                                                                                              GAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAA                                                      >  SRR19973065.2919262/1‑150 (MQ=60)
                                                                                              GAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAA                                                      >  SRR19973065.1948711/1‑150 (MQ=60)
                                                                                               AAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTagatcggaaga                                                     >  SRR19973065.1144014/1‑139 (MQ=60)
                                                                                               AAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAA                                                     >  SRR19973065.3493302/1‑150 (MQ=60)
                                                                                                AAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAAC                                                    <  SRR19973065.649116/150‑1 (MQ=60)
                                                                                                AAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAAC                                                    <  SRR19973065.3396713/150‑1 (MQ=60)
                                                                                                 AGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACC                                                   >  SRR19973065.3191827/1‑150 (MQ=60)
                                                                                                 AGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACC                                                   >  SRR19973065.3291586/1‑150 (MQ=60)
                                                                                                 AGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACC                                                   <  SRR19973065.3354992/150‑1 (MQ=60)
                                                                                                 AGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACC                                                   <  SRR19973065.2427218/150‑1 (MQ=60)
                                                                                                 AGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACC                                                   <  SRR19973065.1255427/150‑1 (MQ=60)
                                                                                                   AACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCA                                                 >  SRR19973065.3197305/1‑150 (MQ=60)
                                                                                                      CGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGAC                                              <  SRR19973065.3014425/150‑1 (MQ=60)
                                                                                                           TCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCA                                         >  SRR19973065.2199760/1‑150 (MQ=60)
                                                                                                           TCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCA                                         >  SRR19973065.2198329/1‑150 (MQ=60)
                                                                                                            CTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCAT                                        >  SRR19973065.2750790/1‑150 (MQ=60)
                                                                                                            CTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCAT                                        >  SRR19973065.2497352/1‑150 (MQ=60)
                                                                                                                  ATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCT                                  <  SRR19973065.1911805/150‑1 (MQ=60)
                                                                                                                  ATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCT                                  <  SRR19973065.1911511/150‑1 (MQ=60)
                                                                                                                  ATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCT                                  <  SRR19973065.2716227/150‑1 (MQ=60)
                                                                                                                     TCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGT                               <  SRR19973065.71357/150‑1 (MQ=60)
                                                                                                                     TCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGT                               <  SRR19973065.71345/150‑1 (MQ=60)
                                                                                                                         CCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCC                           <  SRR19973065.1008051/150‑1 (MQ=60)
                                                                                                                         CCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCC                           <  SRR19973065.1008057/150‑1 (MQ=60)
                                                                                                                         CCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCC                           <  SRR19973065.2772729/150‑1 (MQ=60)
                                                                                                                         CCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCC                           <  SRR19973065.2772712/150‑1 (MQ=60)
                                                                                                                           CGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCAT                         >  SRR19973065.163489/1‑150 (MQ=60)
                                                                                                                           CGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCAT                         >  SRR19973065.2747864/1‑150 (MQ=60)
                                                                                                                           CGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCAT                         >  SRR19973065.2747865/1‑150 (MQ=60)
                                                                                                                           CGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGGTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCAT                         >  SRR19973065.2716492/1‑150 (MQ=60)
                                                                                                                            GGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATC                        >  SRR19973065.2270813/1‑150 (MQ=60)
                                                                                                                                AATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTAGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTC                    >  SRR19973065.109295/1‑150 (MQ=60)
                                                                                                                                   GCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCT                 >  SRR19973065.2096170/1‑150 (MQ=60)
                                                                                                                                   GCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCT                 <  SRR19973065.966447/150‑1 (MQ=60)
                                                                                                                                       GACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAAT             <  SRR19973065.3195860/150‑1 (MQ=60)
                                                                                                                                       GACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAAT             <  SRR19973065.1407059/150‑1 (MQ=60)
                                                                                                                                        ACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATT            <  SRR19973065.2558641/150‑1 (MQ=60)
                                                                                                                                        ACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATT            <  SRR19973065.1782127/150‑1 (MQ=60)
                                                                                                                                           GGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATC         <  SRR19973065.2232112/150‑1 (MQ=60)
                                                                                                                                             TCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAG       <  SRR19973065.1837923/150‑1 (MQ=60)
                                                                                                                                               CATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGAA     >  SRR19973065.681999/1‑150 (MQ=60)
                                                                                                                                                 TTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGAAAA   >  SRR19973065.312298/1‑150 (MQ=60)
                                                                                                                                                  TACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGAAAAA  <  SRR19973065.1654/150‑1 (MQ=60)
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TGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGAAAAA  >  CP009273/1430727‑1431022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑