Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,430,873 G→T 94.3% S93* (TCA→TAA)  ompN ← outer membrane pore protein N, non‑specific

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,430,8730GT94.3% 574.4 / 24.1 204S93* (TCA→TAA) ompNouter membrane pore protein N, non‑specific
Reads supporting (aligned to +/- strand):  ref base G (5/7);  new base T (100/92);  total (105/99)
Fisher's exact test for biased strand distribution p-value = 5.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 204 total aligned reads displayed.

TGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCA  >  CP009273/1430731‑1431016
                                                                                                                                              |                                                                                                                                               
   aTTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGttt                                                                                                                                         <  1:3056470/148‑1 (MQ=255)
    ttGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGtttt                                                                                                                                        <  1:2445057/148‑1 (MQ=255)
    ttGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGtttt                                                                                                                                        <  1:284869/148‑1 (MQ=255)
    ttGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGtttt                                                                                                                                        >  2:2160700/1‑148 (MQ=255)
    ttGGTATAAGAGTCACCGCCAAATTCAGGCAGAATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGtttt                                                                                                                                        >  2:1293276/1‑148 (MQ=255)
     tGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGttttt                                                                                                                                       <  2:1863441/148‑1 (MQ=255)
         ataAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGaa                                                                                                                                   <  1:1036622/148‑1 (MQ=255)
          taAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAg                                                                                                                                  <  1:2482948/148‑1 (MQ=255)
            agagTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGAt                                                                                                                                >  1:271825/1‑148 (MQ=255)
             gagTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGAtt                                                                                                                               >  2:2788333/1‑148 (MQ=255)
                tCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAg                                                                                                                            <  1:3478150/148‑1 (MQ=255)
                  aCCGCCTAATTCTGGCAGCATATCGGTCCAGCCTTCGATGTCTTGCATTACGCCATAATTACGTACGTAATCGAAAGAACCGTAATCTGAAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAgtg                                                                                                                          <  1:248078/148‑1 (MQ=255)
                   ccGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACTCCATAATTACGTCCGTAATAGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAgtgt                                                                                                                         <  1:168362/148‑1 (MQ=255)
                       cAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTgttgtt                                                                                                                     >  1:3335748/1‑148 (MQ=255)
                        aaaTTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGttt                                                                                                                    <  2:464559/148‑1 (MQ=255)
                         aaTTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTg                                                                                                                   >  1:2140626/1‑148 (MQ=255)
                          aTTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGc                                                                                                                  <  1:1482029/148‑1 (MQ=255)
                           ttCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGcc                                                                                                                 <  1:488860/148‑1 (MQ=255)
                                  agcaTATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAatat                                                                                                          <  2:2459750/148‑1 (MQ=255)
                                   gcaTATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATAtt                                                                                                         <  2:953486/148‑1 (MQ=255)
                                      tatCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTa                                                                                                      <  1:779887/148‑1 (MQ=255)
                                       atCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTAt                                                                                                     >  2:3529433/1‑148 (MQ=255)
                                       atCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTAt                                                                                                     >  2:601490/1‑148 (MQ=255)
                                         cGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTc                                                                                                   <  1:107388/148‑1 (MQ=255)
                                         cGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTc                                                                                                   <  1:2250993/148‑1 (MQ=255)
                                           gTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTccc                                                                                                 <  1:1978510/148‑1 (MQ=255)
                                            tCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCa                                                                                                <  1:1259585/148‑1 (MQ=255)
                                            tCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCa                                                                                                <  2:2657363/148‑1 (MQ=255)
                                            tCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCa                                                                                                <  1:1933807/148‑1 (MQ=255)
                                            tCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCa                                                                                                >  1:707146/1‑148 (MQ=255)
                                                gCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGa                                                                                            >  2:786751/1‑148 (MQ=255)
                                                gCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGa                                                                                            >  2:2342403/1‑148 (MQ=255)
                                                gCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGa                                                                                            >  2:1988851/1‑148 (MQ=255)
                                                 ccTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAc                                                                                           >  1:1191840/1‑148 (MQ=255)
                                                 ccTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAc                                                                                           >  1:1591739/1‑148 (MQ=255)
                                                 ccTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAc                                                                                           >  2:3295415/1‑148 (MQ=255)
                                                     cGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTa                                                                                       <  2:3512479/148‑1 (MQ=255)
                                                           cGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGt                                                                                 >  1:2216449/1‑148 (MQ=255)
                                                              aCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAg                                                                              <  2:1073450/148‑1 (MQ=255)
                                                              aCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATAGTGTATTCCCATTGACCGTAGCCAGTGAg                                                                              >  1:869685/1‑148 (MQ=255)
                                                                aTTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGtt                                                                            <  2:3365115/148‑1 (MQ=255)
                                                                 ttACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTg                                                                           >  2:2375045/1‑148 (MQ=255)
                                                                  tACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGa                                                                          <  1:3542975/148‑1 (MQ=255)
                                                                      ccATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGt                                                                      <  1:3215303/148‑1 (MQ=255)
                                                                        aTAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTa                                                                    <  2:1711207/148‑1 (MQ=255)
                                                                        aTAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTa                                                                    >  1:2596746/1‑148 (MQ=255)
                                                                        aTAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTa                                                                    >  1:41916/1‑148 (MQ=255)
                                                                          aaTTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAt                                                                  <  1:518024/148‑1 (MQ=255)
                                                                          aaTTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAt                                                                  >  2:2826752/1‑148 (MQ=255)
                                                                           aTTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAtt                                                                 >  1:1617915/1‑148 (MQ=255)
                                                                           aTTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAtt                                                                 >  1:2303666/1‑148 (MQ=255)
                                                                            ttACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAttt                                                                >  2:1569057/1‑148 (MQ=255)
                                                                             tACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTg                                                               <  1:3023511/148‑1 (MQ=255)
                                                                             tACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTg                                                               <  1:953704/148‑1 (MQ=255)
                                                                                gTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGt                                                            <  1:3201238/148‑1 (MQ=255)
                                                                                gTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGt                                                            >  2:696607/1‑148 (MQ=255)
                                                                                gTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGt                                                            <  2:620621/148‑1 (MQ=255)
                                                                                 tCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGtt                                                           >  2:3354435/1‑148 (MQ=255)
                                                                                  ccGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGttt                                                          <  2:780778/148‑1 (MQ=255)
                                                                                  ccGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGttt                                                          <  2:1989215/148‑1 (MQ=255)
                                                                                     tAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGc                                                       <  1:1986822/148‑1 (MQ=255)
                                                                                              gAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAAc                                              >  1:688163/1‑148 (MQ=255)
                                                                                                aCCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTTCCTGAATATTGTATTCCCATTGACCGTATCCAGTGAGTTGATCGTTAATTTGGGTTTAGCCTTTAAAAccc                                            >  1:25174/1‑148 (MQ=255)
                                                                                                    tAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGAc                                        >  2:2139611/1‑148 (MQ=255)
                                                                                                       tCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGAcgcg                                     >  1:2225128/1‑148 (MQ=255)
                                                                                                        cTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACgcgc                                    >  1:2831332/1‑148 (MQ=255)
                                                                                                        cTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACgcgc                                    >  1:2754686/1‑148 (MQ=255)
                                                                                                        cTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACgcgc                                    >  2:506562/1‑148 (MQ=255)
                                                                                                        cTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACgcgc                                    >  2:219943/1‑148 (MQ=255)
                                                                                                          gCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCAt                                  <  2:2938275/148‑1 (MQ=255)
                                                                                                          gCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCAt                                  >  1:278132/1‑148 (MQ=255)
                                                                                                            aaaTTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAg                                >  1:351238/1‑148 (MQ=255)
                                                                                                            aaaTTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAg                                <  2:2518704/148‑1 (MQ=255)
                                                                                                             aaTTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGc                               >  1:2927196/1‑148 (MQ=255)
                                                                                                              aTTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGct                              <  2:145994/148‑1 (MQ=255)
                                                                                                               tttCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGctc                             >  1:2107647/1‑148 (MQ=255)
                                                                                                               tttCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGctc                             <  2:1256378/148‑1 (MQ=255)
                                                                                                               tttCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGctc                             <  2:3034994/148‑1 (MQ=255)
                                                                                                               tttCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTAGCCTTTAAAACCCAGACGCGCATAGctc                             >  2:1788111/1‑148 (MQ=255)
                                                                                                                 tCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTg                           >  1:3472189/1‑148 (MQ=255)
                                                                                                                  cAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTgg                          >  1:3236018/1‑148 (MQ=255)
                                                                                                                  cAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTgg                          <  2:2781977/148‑1 (MQ=255)
                                                                                                                         gCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCAt                   >  2:2357589/1‑148 (MQ=255)
                                                                                                                         gCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCAt                   >  2:568101/1‑148 (MQ=255)
                                                                                                                          cAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATc                  <  2:3093073/148‑1 (MQ=255)
                                                                                                                          cAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATc                  <  2:1156923/148‑1 (MQ=255)
                                                                                                                           aaaTGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCt                 <  1:2370158/148‑1 (MQ=255)
                                                                                                                            aaggCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCtt                <  2:3558527/145‑1 (MQ=255)
                                                                                                                             aTGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCttt               <  2:2238314/148‑1 (MQ=255)
                                                                                                                             aTGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCttt               >  1:2638387/1‑148 (MQ=255)
                                                                                                                             aTGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCttt               >  1:1827260/1‑148 (MQ=255)
                                                                                                                               gCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCg             >  2:2158211/1‑148 (MQ=255)
                                                                                                                               gCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCg             <  1:2207088/148‑1 (MQ=255)
                                                                                                                                   gACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGa         <  1:2301901/148‑1 (MQ=255)
                                                                                                                                   gACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGa         <  2:924549/148‑1 (MQ=255)
                                                                                                                                    aCGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGaa        >  2:3173828/1‑148 (MQ=255)
                                                                                                                                      gggTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAAtt      <  2:519747/148‑1 (MQ=255)
                                                                                                                                       ggTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTa     <  2:1216419/148‑1 (MQ=255)
                                                                                                                                       ggTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTa     <  2:2994341/148‑1 (MQ=255)
                                                                                                                                        gTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTAt    <  1:3173828/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                               
TGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCA  >  CP009273/1430731‑1431016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGAAAAA  >  CP009273/1430727‑1431022
                                                                                                                                                  |                                                                                                                                                     
TGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACT                                                                                                                                                    <  SRR19973066.920095/150‑1 (MQ=60)
       ATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTT                                                                                                                                             <  SRR19973066.214425/150‑1 (MQ=60)
       ATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTT                                                                                                                                             >  SRR19973066.846271/1‑150 (MQ=60)
       ATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTT                                                                                                                                             >  SRR19973066.1919304/1‑150 (MQ=60)
       ATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTT                                                                                                                                             <  SRR19973066.3076909/150‑1 (MQ=60)
        TTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTT                                                                                                                                            <  SRR19973066.2461710/150‑1 (MQ=60)
        TTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTT                                                                                                                                            <  SRR19973066.286926/150‑1 (MQ=60)
             ATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGA                                                                                                                                       <  SRR19973066.1044248/150‑1 (MQ=60)
             ATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGA                                                                                                                                       >  SRR19973066.3430566/1‑150 (MQ=60)
             ATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGA                                                                                                                                       >  SRR19973066.2094710/1‑150 (MQ=60)
              TAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGAT                                                                                                                                      >  SRR19973066.273837/1‑150 (MQ=60)
              TAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGAT                                                                                                                                      <  SRR19973066.2499887/150‑1 (MQ=60)
                AGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTC                                                                                                                                    >  SRR19973066.3219690/1‑150 (MQ=60)
                   GTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGT                                                                                                                                 <  SRR19973066.2720206/150‑1 (MQ=60)
                    TCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTG                                                                                                                                <  SRR19973066.3501363/150‑1 (MQ=60)
                      ACCGCCTAATTCTGGCAGCATATCGGTCCAGCCTTCGATGTCTTGCATTACGCCATAATTACGTACGTAATCGAAAGAACCGTAATCTGAAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTT                                                                                                                              <  SRR19973066.249993/150‑1 (MQ=60)
                       CCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACTCCATAATTACGTCCGTAATAGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTG                                                                                                                             <  SRR19973066.169877/150‑1 (MQ=60)
                         GCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTT                                                                                                                           <  SRR19973066.1950794/150‑1 (MQ=60)
                         GCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTT                                                                                                                           >  SRR19973066.3357943/1‑150 (MQ=60)
                           CAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTG                                                                                                                         >  SRR19973066.2155481/1‑150 (MQ=60)
                             gttagtgactggagttcagacgtgtgctcttccgatctccTTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCC                                                                                                                       <  SRR19973066.600364/110‑1 (MQ=60)
                              ATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCT                                                                                                                      <  SRR19973066.1492441/150‑1 (MQ=60)
                               TTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTG                                                                                                                     <  SRR19973066.492525/150‑1 (MQ=60)
                               TTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTG                                                                                                                     <  SRR19973066.477852/150‑1 (MQ=60)
                                  AGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAAT                                                                                                                  <  SRR19973066.1643787/150‑1 (MQ=60)
                                  AGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAAT                                                                                                                  >  SRR19973066.2797381/1‑150 (MQ=60)
                                      AGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTG                                                                                                              >  SRR19973066.3147422/1‑150 (MQ=60)
                                        CATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTA                                                                                                            >  SRR19973066.2914575/1‑150 (MQ=60)
                                          TATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATT                                                                                                          <  SRR19973066.2583716/150‑1 (MQ=60)
                                          TATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATT                                                                                                          <  SRR19973066.785644/150‑1 (MQ=60)
                                            TCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCC                                                                                                        <  SRR19973066.3571252/150‑1 (MQ=60)
                                             CGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCC                                                                                                       <  SRR19973066.108497/150‑1 (MQ=60)
                                             CGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCC                                                                                                       <  SRR19973066.2266511/150‑1 (MQ=60)
                                              GGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCA                                                                                                      >  SRR19973066.712379/1‑150 (MQ=60)
                                               GTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCAT                                                                                                     <  SRR19973066.1992483/150‑1 (MQ=60)
                                               GTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCAT                                                                                                     >  SRR19973066.698897/1‑150 (MQ=60)
                                                TCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATT                                                                                                    <  SRR19973066.1268668/150‑1 (MQ=60)
                                                TCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATT                                                                                                    <  SRR19973066.1947531/150‑1 (MQ=60)
                                                  CAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGA                                                                                                  >  SRR19973066.1716115/1‑150 (MQ=60)
                                                   AGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAC                                                                                                 >  SRR19973066.1200183/1‑150 (MQ=60)
                                                   AGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAC                                                                                                 >  SRR19973066.1603261/1‑150 (MQ=60)
                                                      CTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGT                                                                                              >  SRR19973066.3501674/1‑150 (MQ=60)
                                                       TTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTA                                                                                             >  SRR19973066.1165148/1‑150 (MQ=60)
                                                        TCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAG                                                                                            <  SRR19973066.253830/150‑1 (MQ=60)
                                                         CGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGC                                                                                           <  SRR19973066.324096/150‑1 (MQ=60)
                                                           ATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCA                                                                                         >  SRR19973066.3335331/1‑150 (MQ=60)
                                                             GTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGT                                                                                       >  SRR19973066.2231816/1‑150 (MQ=60)
                                                             GTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGT                                                                                       >  SRR19973066.2231793/1‑150 (MQ=60)
                                                               CGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGA                                                                                     <  SRR19973066.969649/150‑1 (MQ=60)
                                                                GTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATAGTGTATTCCCATTGACCGTAGCCAGTGAG                                                                                    >  SRR19973066.875917/1‑150 (MQ=60)
                                                                      TACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATC                                                                              <  SRR19973066.3566505/150‑1 (MQ=60)
                                                                          CCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTA                                                                          <  SRR19973066.3236595/150‑1 (MQ=60)
                                                                          CCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTA                                                                          >  SRR19973066.42478/1‑150 (MQ=60)
                                                                          CCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTA                                                                          >  SRR19973066.2614328/1‑150 (MQ=60)
                                                                            ATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAT                                                                        >  SRR19973066.777070/1‑150 (MQ=60)
                                                                             TAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATT                                                                       >  SRR19973066.1629649/1‑150 (MQ=60)
                                                                             TAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATT                                                                       >  SRR19973066.1629650/1‑150 (MQ=60)
                                                                             TAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATT                                                                       <  SRR19973066.3497010/150‑1 (MQ=60)
                                                                             TAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATT                                                                       >  SRR19973066.2319440/1‑150 (MQ=60)
                                                                              AATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTT                                                                      <  SRR19973066.521840/150‑1 (MQ=60)
                                                                              AATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTT                                                                      <  SRR19973066.521771/150‑1 (MQ=60)
                                                                                 TACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGG                                                                   <  SRR19973066.2525038/150‑1 (MQ=60)
                                                                                 TACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGG                                                                   <  SRR19973066.960673/150‑1 (MQ=60)
                                                                                 TACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGG                                                                   <  SRR19973066.3043687/150‑1 (MQ=60)
                                                                                 TACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGG                                                                   >  SRR19973066.302984/1‑150 (MQ=60)
                                                                                    GTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTT                                                                <  SRR19973066.3222534/150‑1 (MQ=60)
                                                                                    GTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTT                                                                <  SRR19973066.3222489/150‑1 (MQ=60)
                                                                                      CCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCG                                                              >  SRR19973066.1290036/1‑150 (MQ=60)
                                                                                        GTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCC                                                            >  SRR19973066.3057334/1‑150 (MQ=60)
                                                                                        GTAATAGAAAGAACCGTAATATGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGTTTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCC                                                            <  SRR19973066.2317392/150‑1 (MQ=60)
                                                                                         TAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCT                                                           <  SRR19973066.2000829/150‑1 (MQ=60)
                                                                                         TAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCT                                                           <  SRR19973066.1999997/150‑1 (MQ=60)
                                                                                          AATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTT                                                          <  SRR19973066.1052465/150‑1 (MQ=60)
                                                                                          AATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTT                                                          <  SRR19973066.2842963/150‑1 (MQ=60)
                                                                                                AAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAAC                                                    >  SRR19973066.693221/1‑150 (MQ=60)
                                                                                                AAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAAC                                                    >  SRR19973066.245654/1‑150 (MQ=60)
                                                                                                AAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAAC                                                    >  SRR19973066.2351804/1‑150 (MQ=60)
                                                                                                  GAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTTCCTGAATATTGTATTCCCATTGACCGTATCCAGTGAGTTGATCGTTAATTTGGGTTTAGCCTTTAAAACCC                                                  >  SRR19973066.25535/1‑150 (MQ=60)
                                                                                                        TAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGC                                            >  SRR19973066.2228470/1‑150 (MQ=60)
                                                                                                         AATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCG                                           <  SRR19973066.833854/150‑1 (MQ=60)
                                                                                                         AATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCG                                           >  SRR19973066.2242255/1‑150 (MQ=60)
                                                                                                         AATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCG                                           >  SRR19973066.2240508/1‑150 (MQ=60)
                                                                                                          ATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGC                                          >  SRR19973066.2773229/1‑150 (MQ=60)
                                                                                                          ATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGC                                          >  SRR19973066.2773235/1‑150 (MQ=60)
                                                                                                          ATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGC                                          >  SRR19973066.2850360/1‑150 (MQ=60)
                                                                                                          ATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCACATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAAACCAGACGCGA                                          >  SRR19973066.2850371/1‑150 (MQ=60)
                                                                                                            CTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCAT                                        >  SRR19973066.280159/1‑150 (MQ=60)
                                                                                                              GCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAG                                      >  SRR19973066.2162197/1‑150 (MQ=60)
                                                                                                              GCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAG                                      >  SRR19973066.353974/1‑150 (MQ=60)
                                                                                                               CAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGC                                     >  SRR19973066.2946692/1‑150 (MQ=60)
                                                                                                                 AATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTC                                   >  SRR19973066.2122193/1‑150 (MQ=60)
                                                                                                                  ATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCT                                  >  SRR19973066.2305223/1‑150 (MQ=60)
                                                                                                                   TTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTG                                 >  SRR19973066.3495377/1‑150 (MQ=60)
                                                                                                                    TTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGG                                >  SRR19973066.3257383/1‑150 (MQ=60)
                                                                                                                             GCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCT                       <  SRR19973066.3552909/150‑1 (MQ=60)
                                                                                                                               AAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTT                     <  SRR19973066.2386185/150‑1 (MQ=60)
                                                                                                                               AAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTT                     >  SRR19973066.1840296/1‑150 (MQ=60)
                                                                                                                               AAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTT                     >  SRR19973066.1513455/1‑150 (MQ=60)
                                                                                                                               AAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTT                     >  SRR19973066.2656142/1‑150 (MQ=60)
                                                                                                                               AAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTT                     >  SRR19973066.2655556/1‑150 (MQ=60)
                                                                                                                                   GCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCT                 >  SRR19973066.880419/1‑150 (MQ=60)
                                                                                                                                   GCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCT                 <  SRR19973066.2222378/150‑1 (MQ=60)
                                                                                                                                   GCCAGACGGGTCCATTACTGGTTTTTTGAAGACTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCT                 >  SRR19973066.1220001/1‑150 (MQ=60)
                                                                                                                                    CCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTG                <  SRR19973066.2496323/150‑1 (MQ=60)
                                                                                                                                    CCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTG                <  SRR19973066.2497862/150‑1 (MQ=60)
                                                                                                                                       GACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAAT             <  SRR19973066.2317666/150‑1 (MQ=60)
                                                                                                                                       GACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAAT             <  SRR19973066.2317672/150‑1 (MQ=60)
                                                                                                                                            GTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGACTATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCA        <  SRR19973066.3194962/150‑1 (MQ=60)
                                                                                                                                                ATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGAAA    >  SRR19973066.2208231/1‑150 (MQ=60)
                                                                                                                                                ATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGAAA    >  SRR19973066.1933743/1‑150 (MQ=60)
                                                                                                                                                  TACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGAAAAA  >  SRR19973066.2445177/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
TGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGAAAAA  >  CP009273/1430727‑1431022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑