Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,430,873 G→T 100% S93* (TCA→TAA)  ompN ← outer membrane pore protein N, non‑specific

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,430,8730GT98.6% 714.4 / 0.8 226S93* (TCA→TAA) ompNouter membrane pore protein N, non‑specific
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base T (121/101);  total (122/104)
Fisher's exact test for biased strand distribution p-value = 3.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 226 total aligned reads displayed.

GTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGA  >  CP009273/1430728‑1431018
                                                                                                                                                 |                                                                                                                                                 
gTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTAc                                                                                                                                                 <  1:387051/148‑1 (MQ=255)
gTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTAc                                                                                                                                                 <  2:1374012/148‑1 (MQ=255)
   tGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTgg                                                                                                                                              <  2:2271378/148‑1 (MQ=255)
       ttGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGtttt                                                                                                                                          >  2:3149831/1‑148 (MQ=255)
            ataAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGaa                                                                                                                                     >  2:3081931/1‑148 (MQ=255)
            ataAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGaa                                                                                                                                     >  1:3401720/1‑148 (MQ=255)
             taAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAg                                                                                                                                    <  2:1665555/148‑1 (MQ=255)
             taAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAg                                                                                                                                    >  1:2146042/1‑148 (MQ=255)
              aaGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGa                                                                                                                                   >  1:1223350/1‑148 (MQ=255)
              aaGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGa                                                                                                                                   <  2:2657728/148‑1 (MQ=255)
              aaGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGa                                                                                                                                   <  1:729427/148‑1 (MQ=255)
               agagTCACCGCCAAATTCAGGCAGCATATCTGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGAt                                                                                                                                  <  2:177646/148‑1 (MQ=255)
                 agTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTc                                                                                                                                >  2:3017179/1‑148 (MQ=255)
                  gTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCa                                                                                                                               <  2:2074987/148‑1 (MQ=255)
                  gTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCa                                                                                                                               <  1:2743933/148‑1 (MQ=255)
                   tCACCGCCTAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATAGAAAGAACCGTAATATGCAAATTTCAGCCCGGCAAAGGCCAGACGGGTCGATTACTGGTTTTTTGAAGATTCAg                                                                                                                              <  1:501261/148‑1 (MQ=255)
                    cACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAgt                                                                                                                             >  1:1032545/1‑148 (MQ=255)
                       cGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGtgtt                                                                                                                          <  1:2096402/148‑1 (MQ=255)
                             aTTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGc                                                                                                                    <  2:1942698/148‑1 (MQ=255)
                             aTTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGc                                                                                                                    >  2:395418/1‑148 (MQ=255)
                               tCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCt                                                                                                                  >  1:2505767/1‑148 (MQ=255)
                                  ggCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGac                                                                                                               >  2:3299039/1‑147 (MQ=255)
                                  ggCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGaa                                                                                                               >  1:441254/1‑148 (MQ=255)
                                  ggCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGaa                                                                                                               <  2:1283236/148‑1 (MQ=255)
                                       caTATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTg                                                                                                          >  2:3528232/1‑148 (MQ=255)
                                         tatCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTa                                                                                                        <  2:1478102/148‑1 (MQ=255)
                                         tatCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTa                                                                                                        <  2:1613551/148‑1 (MQ=255)
                                          atCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTAt                                                                                                       <  2:608274/148‑1 (MQ=255)
                                           tCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGATTATTGTAtt                                                                                                      >  2:2463907/1‑148 (MQ=255)
                                           tCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTAtt                                                                                                      >  2:461049/1‑148 (MQ=255)
                                             ggTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTcc                                                                                                    <  2:2757716/148‑1 (MQ=255)
                                             ggTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTcc                                                                                                    <  2:1919895/148‑1 (MQ=255)
                                               tCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCa                                                                                                  <  2:2638047/148‑1 (MQ=255)
                                                ccAGCCTTCGATGTCGTAAATTACGCCATAATTACGTCAGTAATCTAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGTGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCAt                                                                                                 <  2:3673099/148‑1 (MQ=255)
                                                  aGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTg                                                                                               <  1:3025403/148‑1 (MQ=255)
                                                     cTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAcc                                                                                            <  1:3275646/148‑1 (MQ=255)
                                                      ttCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCg                                                                                           >  1:824155/1‑148 (MQ=255)
                                                      ttCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATAGTGTATTCCCATTGACCg                                                                                           >  1:3406773/1‑148 (MQ=255)
                                                       tCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGt                                                                                          >  1:1638522/1‑148 (MQ=255)
                                                           tGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGcc                                                                                      >  1:2084182/1‑148 (MQ=255)
                                                           tGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGcc                                                                                      <  1:2905443/148‑1 (MQ=255)
                                                              cGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGt                                                                                   <  1:1005012/148‑1 (MQ=255)
                                                               gTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTg                                                                                  <  2:1034198/148‑1 (MQ=255)
                                                                tACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGa                                                                                 >  1:2847960/1‑148 (MQ=255)
                                                                tACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGa                                                                                 >  2:3534251/1‑148 (MQ=255)
                                                                 aCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAg                                                                                >  2:3633310/1‑148 (MQ=255)
                                                                    ttACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTg                                                                             >  2:1876134/1‑148 (MQ=255)
                                                                    ttACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTg                                                                             >  2:1382192/1‑148 (MQ=255)
                                                                     tACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGa                                                                            <  2:2337286/148‑1 (MQ=255)
                                                                          cATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGtt                                                                       >  2:3098219/1‑148 (MQ=255)
                                                                             aaTTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAt                                                                    <  2:3467038/148‑1 (MQ=255)
                                                                             aaTTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAt                                                                    >  1:2650917/1‑148 (MQ=255)
                                                                             aaTTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAt                                                                    >  1:2434401/1‑148 (MQ=255)
                                                                             aaTTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCAGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAt                                                                    >  1:1533900/1‑148 (MQ=255)
                                                                              aTTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAtt                                                                   <  1:3532521/148‑1 (MQ=255)
                                                                              aTTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAtt                                                                   <  1:3527558/148‑1 (MQ=255)
                                                                               ttACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAttt                                                                  >  1:337312/1‑148 (MQ=255)
                                                                                tACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTg                                                                 >  1:1470887/1‑148 (MQ=255)
                                                                                tACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTg                                                                 <  1:639611/148‑1 (MQ=255)
                                                                                  cGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTggg                                                               >  2:1411163/1‑148 (MQ=255)
                                                                                    tCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGtt                                                             >  1:877662/1‑148 (MQ=255)
                                                                                        tAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGc                                                         >  2:781292/1‑148 (MQ=255)
                                                                                        tAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGc                                                         <  2:2996883/148‑1 (MQ=255)
                                                                                         aaTCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGcc                                                        <  2:571881/148‑1 (MQ=255)
                                                                                          aTCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCt                                                       <  2:3573272/148‑1 (MQ=255)
                                                                                          aTCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCt                                                       <  2:2709804/148‑1 (MQ=255)
                                                                                          aTCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCt                                                       <  1:236197/148‑1 (MQ=255)
                                                                                                aGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTaaaa                                                 >  1:1389634/1‑148 (MQ=255)
                                                                                                aGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTaaaa                                                 >  1:916178/1‑148 (MQ=255)
                                                                                                  aaCCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAAcc                                               <  2:1693331/148‑1 (MQ=255)
                                                                                                    ccGTAATCTGCAAATTTCAGCCCGCCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCa                                             >  1:2303868/1‑148 (MQ=255)
                                                                                                     cGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAg                                            <  2:3361757/148‑1 (MQ=255)
                                                                                                        aaTCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTTAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTATCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCATAct                                         >  2:1608633/1‑147 (MQ=255)
                                                                                                             gCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCAt                                    >  1:1519221/1‑148 (MQ=255)
                                                                                                             gCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCAt                                    >  2:524479/1‑148 (MQ=255)
                                                                                                               aaaTTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAg                                  <  2:3549358/148‑1 (MQ=255)
                                                                                                                 aTTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGct                                >  2:3688425/1‑148 (MQ=255)
                                                                                                                  tttCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGctc                               >  2:3447681/1‑148 (MQ=255)
                                                                                                                   ttCAGCCCGGCACATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGctct                              <  2:2276271/148‑1 (MQ=255)
                                                                                                                   ttCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGctct                              >  2:2822349/1‑148 (MQ=255)
                                                                                                                             cAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATc                    >  1:2646104/1‑148 (MQ=255)
                                                                                                                             cAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATc                    >  2:2433430/1‑148 (MQ=255)
                                                                                                                             cAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATc                    >  1:3487148/1‑148 (MQ=255)
                                                                                                                             cAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATc                    >  1:3700024/1‑148 (MQ=255)
                                                                                                                             cAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATc                    <  1:513786/148‑1 (MQ=255)
                                                                                                                             cAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATc                    <  1:560393/148‑1 (MQ=255)
                                                                                                                             cAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATc                    <  2:1155593/148‑1 (MQ=255)
                                                                                                                               aaTGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCtt                  >  2:3086377/1‑148 (MQ=255)
                                                                                                                                aTGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGATGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAAACCACACGCGCATAGATCTGGTCGCCATCttt                 >  1:3797869/1‑148 (MQ=255)
                                                                                                                                  gCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCg               <  2:2698675/148‑1 (MQ=255)
                                                                                                                                      gACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGa           >  1:602854/1‑148 (MQ=255)
                                                                                                                                      gACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGa           >  1:602960/1‑148 (MQ=255)
                                                                                                                                      gACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGa           <  1:3170109/148‑1 (MQ=255)
                                                                                                                                      gACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGa           >  2:6364/1‑148 (MQ=255)
                                                                                                                                       aCGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGaa          >  1:822846/1‑148 (MQ=255)
                                                                                                                                       aCGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGaa          >  2:2177231/1‑148 (MQ=255)
                                                                                                                                       aCGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGaa          >  1:2958476/1‑148 (MQ=255)
                                                                                                                                        cGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAAt         <  2:1545110/148‑1 (MQ=255)
                                                                                                                                          ggTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTa       <  1:2881331/148‑1 (MQ=255)
                                                                                                                                               aTGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGa  <  1:2575505/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGA  >  CP009273/1430728‑1431018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGAAA  >  CP009273/1430724‑1431020
                                                                                                                                                     |                                                                                                                                                   
AGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATT                                                                                                                                                     >  SRR19973069.2740526/1‑150 (MQ=60)
    GTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTG                                                                                                                                                 <  SRR19973069.389930/150‑1 (MQ=60)
    GTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTG                                                                                                                                                 <  SRR19973069.389890/150‑1 (MQ=60)
    GTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTG                                                                                                                                                 <  SRR19973069.389826/150‑1 (MQ=60)
      CTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGT                                                                                                                                               <  SRR19973069.2894322/150‑1 (MQ=60)
      CTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGT                                                                                                                                               >  SRR19973069.2971446/1‑150 (MQ=60)
      CTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGT                                                                                                                                               >  SRR19973069.2998835/1‑150 (MQ=60)
       TGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCACTACTGGTT                                                                                                                                              <  SRR19973069.2613532/150‑1 (MQ=60)
        GCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTT                                                                                                                                             <  SRR19973069.3427944/150‑1 (MQ=60)
              GTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAA                                                                                                                                       >  SRR19973069.3423906/1‑150 (MQ=60)
              GTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAA                                                                                                                                       >  SRR19973069.3423896/1‑150 (MQ=60)
               TATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAG                                                                                                                                      >  SRR19973069.2160761/1‑150 (MQ=60)
                ATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGA                                                                                                                                     >  SRR19973069.3335126/1‑150 (MQ=60)
                ATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGA                                                                                                                                     >  SRR19973069.1422811/1‑150 (MQ=60)
                ATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGA                                                                                                                                     >  SRR19973069.1231710/1‑150 (MQ=60)
                  AAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATT                                                                                                                                   <  SRR19973069.734583/150‑1 (MQ=60)
                      GTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGT                                                                                                                               <  SRR19973069.2762266/150‑1 (MQ=60)
                      GTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGT                                                                                                                               >  SRR19973069.1039680/1‑150 (MQ=60)
                      GTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGT                                                                                                                               >  SRR19973069.1039703/1‑150 (MQ=60)
                       TCACCGCCTAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATAGAAAGAACCGTAATATGCAAATTTCAGCCCGGCAAAGGCCAGACGGGTCGATTACTGGTTTTTTGAAGATTCAGTG                                                                                                                              <  SRR19973069.504856/150‑1 (MQ=60)
                           CGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGT                                                                                                                          <  SRR19973069.2110888/150‑1 (MQ=60)
                                 ATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCT                                                                                                                    >  SRR19973069.2522484/1‑150 (MQ=60)
                                 ATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCT                                                                                                                    >  SRR19973069.2522449/1‑150 (MQ=60)
                                    CAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAA                                                                                                                 >  SRR19973069.444482/1‑150 (MQ=60)
                                     AGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAAT                                                                                                                <  SRR19973069.528488/150‑1 (MQ=60)
                                                    CCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTG                                                                                                 >  SRR19973069.1163648/1‑150 (MQ=60)
                                                      AGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAC                                                                                               <  SRR19973069.3045470/150‑1 (MQ=60)
                                                        CCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCG                                                                                             >  SRR19973069.2702527/1‑150 (MQ=60)
                                                        CCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCG                                                                                             >  SRR19973069.829954/1‑150 (MQ=60)
                                                        CCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCG                                                                                             >  SRR19973069.829970/1‑150 (MQ=60)
                                                        CCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATAGTGTATTCCCATTGACCG                                                                                             >  SRR19973069.3428985/1‑150 (MQ=60)
                                                         CTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGT                                                                                            <  SRR19973069.3297095/150‑1 (MQ=60)
                                                         CTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGT                                                                                            >  SRR19973069.1649766/1‑150 (MQ=60)
                                                          TTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTA                                                                                           >  SRR19973069.718100/1‑150 (MQ=60)
                                                            CGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGC                                                                                         >  SRR19973069.1540415/1‑150 (MQ=60)
                                                             GATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCC                                                                                        >  SRR19973069.2098605/1‑150 (MQ=60)
                                                               TGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAG                                                                                      <  SRR19973069.2924646/150‑1 (MQ=60)
                                                               TGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAG                                                                                      <  SRR19973069.2924593/150‑1 (MQ=60)
                                                                  CGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGA                                                                                   <  SRR19973069.1011918/150‑1 (MQ=60)
                                                                  CGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGA                                                                                   >  SRR19973069.2866728/1‑150 (MQ=60)
                                                                  CGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGA                                                                                   >  SRR19973069.2866726/1‑150 (MQ=60)
                                                                     ACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTT                                                                                >  SRR19973069.907868/1‑150 (MQ=60)
                                                                     ACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTT                                                                                >  SRR19973069.3397998/1‑150 (MQ=60)
                                                                      CATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTG                                                                               >  SRR19973069.3282280/1‑150 (MQ=60)
                                                                               ATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAT                                                                      <  SRR19973069.1393367/150‑1 (MQ=60)
                                                                               ATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAT                                                                      >  SRR19973069.2450812/1‑150 (MQ=60)
                                                                               ATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAT                                                                      >  SRR19973069.2668679/1‑150 (MQ=60)
                                                                               ATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAT                                                                      >  SRR19973069.2803890/1‑150 (MQ=60)
                                                                               ATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAT                                                                      <  SRR19973069.3478086/150‑1 (MQ=60)
                                                                               ATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAT                                                                      >  SRR19973069.2668630/1‑150 (MQ=60)
                                                                               ATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAT                                                                      >  SRR19973069.512333/1‑150 (MQ=60)
                                                                               ATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCAGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAT                                                                      >  SRR19973069.1544435/1‑150 (MQ=60)
                                                                               ATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCAGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAT                                                                      >  SRR19973069.498467/1‑150 (MQ=60)
                                                                                 AATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTT                                                                    >  SRR19973069.234930/1‑150 (MQ=60)
                                                                                 AATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTT                                                                    >  SRR19973069.339666/1‑150 (MQ=60)
                                                                                  ATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTG                                                                   <  SRR19973069.3555405/150‑1 (MQ=60)
                                                                                  ATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTG                                                                   <  SRR19973069.3550375/150‑1 (MQ=60)
                                                                                  ATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTG                                                                   >  SRR19973069.1555688/1‑150 (MQ=60)
                                                                                  ATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTG                                                                   >  SRR19973069.1481141/1‑150 (MQ=60)
                                                                                    TACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGG                                                                 <  SRR19973069.642992/150‑1 (MQ=60)
                                                                                    TACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGG                                                                 <  SRR19973069.643815/150‑1 (MQ=60)
                                                                                      CGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTT                                                               >  SRR19973069.575838/1‑150 (MQ=60)
                                                                                      CGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTT                                                               <  SRR19973069.3140952/150‑1 (MQ=60)
                                                                                      CGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTT                                                               >  SRR19973069.883757/1‑150 (MQ=60)
                                                                                       GTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTT                                                              >  SRR19973069.404917/1‑150 (MQ=60)
                                                                                            TAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCT                                                         >  SRR19973069.2099518/1‑150 (MQ=60)
                                                                                             AATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTT                                                        >  SRR19973069.1876679/1‑150 (MQ=60)
                                                                                             AATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTT                                                        >  SRR19973069.809007/1‑150 (MQ=60)
                                                                                             AATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTT                                                        <  SRR19973069.2091409/150‑1 (MQ=60)
                                                                                             AATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTT                                                        <  SRR19973069.2533043/150‑1 (MQ=60)
                                                                                             AATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTT                                                        >  SRR19973069.2211689/1‑150 (MQ=60)
                                                                                              ATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTT                                                       <  SRR19973069.237984/150‑1 (MQ=60)
                                                                                                  AAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAA                                                   >  SRR19973069.922429/1‑150 (MQ=60)
                                                                                                  AAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAA                                                   >  SRR19973069.1399325/1‑150 (MQ=60)
                                                                                                     GAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCC                                                >  SRR19973069.854596/1‑150 (MQ=60)
                                                                                                      AACCGTAATCTGCAAATTTCAGCCCGCCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCA                                               >  SRR19973069.2319536/1‑150 (MQ=60)
                                                                                                        CCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGA                                             >  SRR19973069.2637316/1‑150 (MQ=60)
                                                                                                         CGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGAC                                            <  SRR19973069.1523475/150‑1 (MQ=60)
                                                                                                            AATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCG                                         >  SRR19973069.3700636/1‑150 (MQ=60)
                                                                                                               CTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCAT                                      >  SRR19973069.1529703/1‑150 (MQ=60)
                                                                                                                          AGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCG                           >  SRR19973069.3521344/1‑150 (MQ=60)
                                                                                                                               GGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATC                      >  SRR19973069.734765/1‑150 (MQ=60)
                                                                                                                               GGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATC                      >  SRR19973069.2438216/1‑150 (MQ=60)
                                                                                                                               GGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATC                      >  SRR19973069.3724209/1‑150 (MQ=60)
                                                                                                                               GGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATC                      >  SRR19973069.3509649/1‑150 (MQ=60)
                                                                                                                               GGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATC                      >  SRR19973069.2663829/1‑150 (MQ=60)
                                                                                                                                GCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCT                     <  SRR19973069.3089994/150‑1 (MQ=60)
                                                                                                                                 CAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTT                    <  SRR19973069.517442/150‑1 (MQ=60)
                                                                                                                                 CAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTT                    <  SRR19973069.564299/150‑1 (MQ=60)
                                                                                                                                 CAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTT                    <  SRR19973069.564318/150‑1 (MQ=60)
                                                                                                                                  AAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGATGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAAACCACACGCGCATAGATCTGGTCGCCATCTTT                   >  SRR19973069.3822474/1‑150 (MQ=60)
                                                                                                                                        CAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGA             >  SRR19973069.1470478/1‑150 (MQ=60)
                                                                                                                                        CAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGA             >  SRR19973069.2331721/1‑150 (MQ=60)
                                                                                                                                        CAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGA             >  SRR19973069.606910/1‑150 (MQ=60)
                                                                                                                                        CAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGA             >  SRR19973069.606919/1‑150 (MQ=60)
                                                                                                                                        CAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGA             >  SRR19973069.607016/1‑150 (MQ=60)
                                                                                                                                         AGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAA            >  SRR19973069.2978106/1‑150 (MQ=60)
                                                                                                                                         AGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAA            >  SRR19973069.828651/1‑150 (MQ=60)
                                                                                                                                          GACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAAT           <  SRR19973069.3190763/150‑1 (MQ=60)
                                                                                                                                             GGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTAT        <  SRR19973069.3551068/150‑1 (MQ=60)
                                                                                                                                             GGGTCCAGTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTAT        <  SRR19973069.3551057/150‑1 (MQ=60)
                                                                                                                                              GGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATC       <  SRR19973069.2900258/150‑1 (MQ=60)
                                                                                                                                                  CATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGAA   >  SRR19973069.140554/1‑150 (MQ=60)
                                                                                                                                                  CATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGAA   <  SRR19973069.2123254/150‑1 (MQ=60)
                                                                                                                                                  CATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGAA   <  SRR19973069.3810217/150‑1 (MQ=60)
                                                                                                                                                  CATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGAA   >  SRR19973069.2917427/1‑150 (MQ=60)
                                                                                                                                                   ATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGAAA  <  SRR19973069.2592598/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
AGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAGAAA  >  CP009273/1430724‑1431020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑