Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,430,873 G→T 100% S93* (TCA→TAA)  ompN ← outer membrane pore protein N, non‑specific

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,430,8730GT98.5% 863.9 / 3.0 268S93* (TCA→TAA) ompNouter membrane pore protein N, non‑specific
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base T (137/126);  total (141/127)
Fisher's exact test for biased strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 268 total aligned reads displayed.

TTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAG  >  CP009273/1430726‑1431017
                                                                                                                                                   |                                                                                                                                                
ttGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTAc                                                                                                                                                >  1:2387298/1‑150 (MQ=255)
 tGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACt                                                                                                                                               >  1:3652864/1‑150 (MQ=255)
  gTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTg                                                                                                                                              >  2:4174385/1‑150 (MQ=255)
     tGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGtt                                                                                                                                           <  2:905391/150‑1 (MQ=255)
     tGCATGGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGtt                                                                                                                                           <  2:135457/150‑1 (MQ=255)
         ttGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGtttttt                                                                                                                                       <  1:1030752/150‑1 (MQ=255)
           ggTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGa                                                                                                                                     <  1:3402231/150‑1 (MQ=255)
            gTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGaa                                                                                                                                    >  1:4632398/1‑150 (MQ=255)
                aaGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGAtt                                                                                                                                <  2:975587/150‑1 (MQ=255)
                aaGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGAtt                                                                                                                                <  2:1780643/150‑1 (MQ=255)
                aaGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGAtt                                                                                                                                >  1:4231839/1‑150 (MQ=255)
                    gTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAgt                                                                                                                            <  1:4123806/150‑1 (MQ=255)
                     tCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAgtg                                                                                                                           <  1:2191695/150‑1 (MQ=255)
                       aCCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGtgtt                                                                                                                         >  2:2885691/1‑150 (MQ=255)
                       aCCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGtgtt                                                                                                                         <  1:5018045/150‑1 (MQ=255)
                       aCCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGtgtt                                                                                                                         <  2:3222533/150‑1 (MQ=255)
                        ccGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGtgttg                                                                                                                        >  2:4383708/1‑150 (MQ=255)
                          gCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTgttgtt                                                                                                                      <  1:3194617/150‑1 (MQ=255)
                           ccAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGttt                                                                                                                     <  2:3844799/150‑1 (MQ=255)
                             aaaTTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGc                                                                                                                   >  2:1100521/1‑150 (MQ=255)
                              aaTTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGcc                                                                                                                  <  2:1757936/150‑1 (MQ=255)
                                   aGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAat                                                                                                             <  2:4911870/150‑1 (MQ=255)
                                    ggCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACTCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAata                                                                                                            <  2:313677/150‑1 (MQ=255)
                                      cagcaTATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATAtt                                                                                                          >  1:1829019/1‑150 (MQ=255)
                                      cagcaTATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATAtt                                                                                                          <  1:3558340/150‑1 (MQ=255)
                                        gcaTATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGt                                                                                                        >  2:1621751/1‑150 (MQ=255)
                                          atatCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTAt                                                                                                      >  2:569734/1‑150 (MQ=255)
                                            atCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTc                                                                                                    >  2:3196005/1‑150 (MQ=255)
                                             tCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTcc                                                                                                   <  1:291251/150‑1 (MQ=255)
                                             tCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTcc                                                                                                   <  1:2582330/150‑1 (MQ=255)
                                             tCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTcc                                                                                                   >  2:214970/1‑150 (MQ=255)
                                                  ccAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTg                                                                                              <  2:1510503/150‑1 (MQ=255)
                                                    aGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAc                                                                                            >  2:2314189/1‑150 (MQ=255)
                                                    aGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAc                                                                                            >  2:1032876/1‑150 (MQ=255)
                                                    aGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAc                                                                                            <  2:1929267/150‑1 (MQ=255)
                                                    aGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAc                                                                                            >  2:4487128/1‑150 (MQ=255)
                                                     gCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAcc                                                                                           >  1:1165270/1‑150 (MQ=255)
                                                     gCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAcc                                                                                           <  1:3142283/150‑1 (MQ=255)
                                                        ttCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTa                                                                                        <  1:4379600/150‑1 (MQ=255)
                                                        ttCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTa                                                                                        >  2:4813719/1‑150 (MQ=255)
                                                        ttCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTa                                                                                        <  2:1231295/150‑1 (MQ=255)
                                                        ttCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTa                                                                                        >  1:628358/1‑150 (MQ=255)
                                                           gATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGcc                                                                                     <  2:413443/150‑1 (MQ=255)
                                                            aTGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCa                                                                                    <  2:5033986/150‑1 (MQ=255)
                                                            aTGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGATTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTATCCa                                                                                    <  1:907045/150‑1 (MQ=255)
                                                             tGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAg                                                                                   >  1:1515434/1‑150 (MQ=255)
                                                                   aCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGtt                                                                             >  2:485658/1‑150 (MQ=255)
                                                                   aCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGtt                                                                             <  1:4212770/150‑1 (MQ=255)
                                                                      tgaCGCCATAATTACGTCCGTAATCGAAAGAACCGGAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGAt                                                                          <  2:2931784/148‑1 (MQ=255)
                                                                             aTAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAt                                                                   >  2:3652470/1‑150 (MQ=255)
                                                                              tAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAtt                                                                  <  1:5085799/150‑1 (MQ=255)
                                                                               aaTTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAAttt                                                                 >  2:1943977/1‑150 (MQ=255)
                                                                                    cGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGtt                                                            <  2:3325875/150‑1 (MQ=255)
                                                                                     gTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGttt                                                           >  1:1646004/1‑150 (MQ=255)
                                                                                      tCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTc                                                          <  2:4073094/150‑1 (MQ=255)
                                                                                      tCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTc                                                          <  2:2426577/150‑1 (MQ=255)
                                                                                         gTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGcc                                                       <  1:1702165/150‑1 (MQ=255)
                                                                                              cGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTna                                                  <  1:2703121/150‑1 (MQ=255)
                                                                                                aaaGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTaaaa                                                >  1:3448078/1‑150 (MQ=255)
                                                                                                 aaGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAAc                                               >  2:4297762/1‑150 (MQ=255)
                                                                                                      ccGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGa                                          >  1:3700644/1‑150 (MQ=255)
                                                                                                       cGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGAc                                         <  1:2264229/150‑1 (MQ=255)
                                                                                                        gTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGAcg                                        <  1:4928533/150‑1 (MQ=255)
                                                                                                        gTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGAcg                                        <  2:3533026/150‑1 (MQ=255)
                                                                                                         tAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGAcgc                                       >  2:3097719/1‑150 (MQ=255)
                                                                                                              tGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATa                                  <  2:2803978/150‑1 (MQ=255)
                                                                                                               gCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAg                                 >  2:3495374/1‑150 (MQ=255)
                                                                                                                cAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGc                                >  1:101237/1‑150 (MQ=255)
                                                                                                                 aaaTTTCAGCACGGCAAATGCCAGACGGGTCCATTACTGGTTTTTAGAAGATACAGTCTTGTTTGCCTCAATAATGTATTCCCATAGACCGAAGCCAGTGAGTTGATAGTTAATTTGGGTTTCGCCTATAAAACCCAGACGCGCATAGct                               >  2:2932661/1‑150 (MQ=255)
                                                                                                                  aaTTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGctc                              >  2:301807/1‑150 (MQ=255)
                                                                                                                  aaTTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGctc                              >  2:5156154/1‑150 (MQ=255)
                                                                                                                     ttCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTgg                           <  2:4953836/150‑1 (MQ=255)
                                                                                                                     ttCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTgg                           >  2:706472/1‑150 (MQ=255)
                                                                                                                       cAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTc                         >  1:3815824/1‑150 (MQ=255)
                                                                                                                        aGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCg                        >  1:3366751/1‑150 (MQ=255)
                                                                                                                        aGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCg                        <  1:4755546/150‑1 (MQ=255)
                                                                                                                        aGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCg                        <  1:2885691/150‑1 (MQ=255)
                                                                                                                        aGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCg                        >  1:1911643/1‑150 (MQ=255)
                                                                                                                          cccGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGcc                      >  1:3478303/1‑150 (MQ=255)
                                                                                                                           ccGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCa                     >  1:774902/1‑150 (MQ=255)
                                                                                                                           ccGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCa                     <  2:3258261/150‑1 (MQ=255)
                                                                                                                           ccGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCa                     >  2:1386058/1‑150 (MQ=255)
                                                                                                                             ggCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATc                   <  1:1481889/150‑1 (MQ=255)
                                                                                                                             ggCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATc                   >  1:3199805/1‑150 (MQ=255)
                                                                                                                                aaaTGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCttt                >  2:1270495/1‑150 (MQ=255)
                                                                                                                                aaaTGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGACTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCttt                <  1:3928173/150‑1 (MQ=255)
                                                                                                                                 aaTGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTc               <  2:3770757/150‑1 (MQ=255)
                                                                                                                                 aaTGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTc               <  1:3684379/150‑1 (MQ=255)
                                                                                                                                  ctGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCg              <  2:2681694/149‑1 (MQ=255)
                                                                                                                                    gCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCt            >  2:3502077/1‑150 (MQ=255)
                                                                                                                                     ccAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTg           >  1:4467451/1‑150 (MQ=255)
                                                                                                                                     ccAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTg           <  1:1232966/150‑1 (MQ=255)
                                                                                                                                      cAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGa          >  2:2519513/1‑150 (MQ=255)
                                                                                                                                         aCGGTTCCATTACTGGTTTTTTGAAGATTCAGAGTTGTTTGCCTGAATATTTTATTCACATTTACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCAAAGCTCTGGTCGCCATCTTTCGCTGAAtt       >  1:129701/1‑150 (MQ=255)
                                                                                                                                          cGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTa      <  2:3611744/150‑1 (MQ=255)
                                                                                                                                          cGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTa      >  2:1262625/1‑150 (MQ=255)
                                                                                                                                          cGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTa      >  1:2958704/1‑150 (MQ=255)
                                                                                                                                           gggTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTAt     >  2:1441765/1‑150 (MQ=255)
                                                                                                                                            ggTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATc    >  1:264313/1‑150 (MQ=255)
                                                                                                                                              tCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAg  <  2:2794748/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATCAG  >  CP009273/1430726‑1431017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATC  >  CP009273/1430724‑1431015
                                                                                                                                                     |                                                                                                                                              
AGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATT                                                                                                                                                <  SRR19973063.4988062/150‑1 (MQ=60)
AGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGCACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATT                                                                                                                                                <  SRR19973063.1418290/150‑1 (MQ=60)
  TTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTAC                                                                                                                                              >  SRR19973063.2394943/1‑150 (MQ=60)
   TGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACT                                                                                                                                             >  SRR19973063.3664576/1‑150 (MQ=60)
     TCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGG                                                                                                                                           >  SRR19973063.843360/1‑150 (MQ=60)
     TCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGG                                                                                                                                           >  SRR19973063.3434992/1‑150 (MQ=60)
       TGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTT                                                                                                                                         >  SRR19973063.4377303/1‑150 (MQ=60)
           TTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTT                                                                                                                                     <  SRR19973063.1033863/150‑1 (MQ=60)
             GGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGA                                                                                                                                   <  SRR19973063.3197915/150‑1 (MQ=60)
             GGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGA                                                                                                                                   <  SRR19973063.3413053/150‑1 (MQ=60)
             GGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGA                                                                                                                                   >  SRR19973063.2605764/1‑150 (MQ=60)
              GTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAA                                                                                                                                  >  SRR19973063.4647201/1‑150 (MQ=60)
               TATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAG                                                                                                                                 >  SRR19973063.3617568/1‑150 (MQ=60)
                 TAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGAGGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGAT                                                                                                                               <  SRR19973063.5130417/150‑1 (MQ=60)
                  AAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATT                                                                                                                              >  SRR19973063.1257013/1‑150 (MQ=60)
                  AAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATT                                                                                                                              >  SRR19973063.4245330/1‑150 (MQ=60)
                  AAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATT                                                                                                                              >  SRR19973063.4208494/1‑150 (MQ=60)
                     AGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAG                                                                                                                           >  SRR19973063.5046064/1‑150 (MQ=60)
                     AGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAG                                                                                                                           >  SRR19973063.2921380/1‑150 (MQ=60)
                     AGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTAGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCAATTACTGGTTTTTTGAAGATTCAG                                                                                                                           >  SRR19973063.5046133/1‑150 (MQ=60)
                      GTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGT                                                                                                                          <  SRR19973063.4137007/150‑1 (MQ=60)
                       TCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTG                                                                                                                         <  SRR19973063.2198678/150‑1 (MQ=60)
                       TCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTG                                                                                                                         <  SRR19973063.2198577/150‑1 (MQ=60)
                        CACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGT                                                                                                                        >  SRR19973063.2914712/1‑150 (MQ=60)
                         ACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTT                                                                                                                       <  SRR19973063.5034238/150‑1 (MQ=60)
                           CGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGT                                                                                                                     >  SRR19973063.4423034/1‑150 (MQ=60)
                            GCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTT                                                                                                                    <  SRR19973063.3204888/150‑1 (MQ=60)
                            GCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTT                                                                                                                    <  SRR19973063.3204941/150‑1 (MQ=60)
                            GCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTT                                                                                                                    >  SRR19973063.639272/1‑150 (MQ=60)
                              CAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTG                                                                                                                  >  SRR19973063.1515135/1‑150 (MQ=60)
                                 ATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCT                                                                                                               >  SRR19973063.2200354/1‑150 (MQ=60)
                                  TTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTG                                                                                                              <  SRR19973063.3396429/150‑1 (MQ=60)
                                   TCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGA                                                                                                             >  SRR19973063.3794677/1‑150 (MQ=60)
                                     AGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCCGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAAT                                                                                                           <  SRR19973063.3202955/150‑1 (MQ=60)
                                        CAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATT                                                                                                        >  SRR19973063.1834711/1‑150 (MQ=60)
                                        CAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATT                                                                                                        <  SRR19973063.3569746/150‑1 (MQ=60)
                                         AGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTG                                                                                                       <  SRR19973063.1395676/150‑1 (MQ=60)
                                          GCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGT                                                                                                      >  SRR19973063.443980/1‑150 (MQ=60)
                                               TCGGTCCATCCTTCGATGTCGTACATTACGCCATAATTACGTCAGTAATCGAAAGAACCGTAATCTGCATATTTCAGCCCGGCAAATGCCAGACGGGTACATTACTGGTTTTTTGATGATTCAGTGTTGTTTGCCTGAATATTGTATTCC                                                                                                 <  SRR19973063.1412774/150‑1 (MQ=60)
                                               TCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCC                                                                                                 <  SRR19973063.2590540/150‑1 (MQ=60)
                                               TCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCC                                                                                                 <  SRR19973063.3271205/150‑1 (MQ=60)
                                               TCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCC                                                                                                 <  SRR19973063.292109/150‑1 (MQ=60)
                                               TCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCC                                                                                                 >  SRR19973063.3690138/1‑150 (MQ=60)
                                                 GGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCA                                                                                               >  SRR19973063.2803734/1‑150 (MQ=60)
                                                 GGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCA                                                                                               >  SRR19973063.2803748/1‑150 (MQ=60)
                                                  GTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCAT                                                                                              <  SRR19973063.3467187/150‑1 (MQ=60)
                                                    CCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTG                                                                                            <  SRR19973063.4065391/150‑1 (MQ=60)
                                                      AGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGAC                                                                                          >  SRR19973063.1016891/1‑150 (MQ=60)
                                                       GCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACC                                                                                         >  SRR19973063.1168784/1‑150 (MQ=60)
                                                       GCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACC                                                                                         <  SRR19973063.3152386/150‑1 (MQ=60)
                                                        CCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCG                                                                                        >  SRR19973063.4824765/1‑150 (MQ=60)
                                                        CCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCG                                                                                        >  SRR19973063.921724/1‑150 (MQ=60)
                                                          TTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTA                                                                                      <  SRR19973063.4393621/150‑1 (MQ=60)
                                                          TTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTA                                                                                      >  SRR19973063.630208/1‑150 (MQ=60)
                                                              ATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCA                                                                                  <  SRR19973063.4806680/150‑1 (MQ=60)
                                                              ATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGATTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTATCCA                                                                                  <  SRR19973063.909701/150‑1 (MQ=60)
                                                               TGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAG                                                                                 <  SRR19973063.3032517/150‑1 (MQ=60)
                                                               TGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAG                                                                                 >  SRR19973063.1520079/1‑150 (MQ=60)
                                                                GTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGT                                                                                <  SRR19973063.422530/150‑1 (MQ=60)
                                                                 TCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTG                                                                               <  SRR19973063.2938681/150‑1 (MQ=60)
                                                                    TACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGT                                                                            >  SRR19973063.3699854/1‑150 (MQ=60)
                                                                     ACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTT                                                                           <  SRR19973063.4226209/150‑1 (MQ=60)
                                                                       ATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGA                                                                         <  SRR19973063.1151510/150‑1 (MQ=60)
                                                                          ACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTTTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCG                                                                      <  SRR19973063.3429887/150‑1 (MQ=60)
                                                                          ACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCG                                                                      <  SRR19973063.1802057/150‑1 (MQ=60)
                                                                           CGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGT                                                                     >  SRR19973063.3489489/1‑150 (MQ=60)
                                                                                TAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATT                                                                <  SRR19973063.5102169/150‑1 (MQ=60)
                                                                                 AATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTT                                                               <  SRR19973063.1036563/150‑1 (MQ=60)
                                                                                 AATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATNCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTT                                                               <  SRR19973063.2687017/150‑1 (MQ=60)
                                                                                       GTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTT                                                         >  SRR19973063.1651033/1‑150 (MQ=60)
                                                                                       GTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTT                                                         <  SRR19973063.449107/150‑1 (MQ=60)
                                                                                         CCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCG                                                       >  SRR19973063.565196/1‑150 (MQ=60)
                                                                                           GTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCC                                                     <  SRR19973063.1707353/150‑1 (MQ=60)
                                                                                             AATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTT                                                   >  SRR19973063.3270432/1‑150 (MQ=60)
                                                                                                CGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTNA                                                <  SRR19973063.2711839/150‑1 (MQ=60)
                                                                                                  AAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAA                                              >  SRR19973063.3459037/1‑150 (MQ=60)
                                                                                                  AAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAA                                              <  SRR19973063.4969991/150‑1 (MQ=60)
                                                                                                      AACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCA                                          >  SRR19973063.1214585/1‑150 (MQ=60)
                                                                                                       ACCGTAATCTGCAAATTTCAGCCAGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACAGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAG                                         >  SRR19973063.1818379/1‑150 (MQ=60)
                                                                                                        CCGTAATCTGCTAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGA                                        <  SRR19973063.1886854/150‑1 (MQ=60)
                                                                                                        CCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGA                                        >  SRR19973063.3712488/1‑150 (MQ=60)
                                                                                                         CGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGAC                                       <  SRR19973063.3756704/150‑1 (MQ=60)
                                                                                                         CGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGAC                                       <  SRR19973063.1537527/150‑1 (MQ=60)
                                                                                                         CGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGAC                                       <  SRR19973063.2271441/150‑1 (MQ=60)
                                                                                                         CGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGAC                                       >  SRR19973063.212230/1‑150 (MQ=60)
                                                                                                          GTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACG                                      <  SRR19973063.4944453/150‑1 (MQ=60)
                                                                                                              TCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCA                                  <  SRR19973063.215615/150‑1 (MQ=60)
                                                                                                                  CAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGC                              >  SRR19973063.101584/1‑150 (MQ=60)
                                                                                                                   AAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCT                             >  SRR19973063.4737457/1‑150 (MQ=60)
                                                                                                                   AAATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCT                             <  SRR19973063.1676992/150‑1 (MQ=60)
                                                                                                                    AATTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTC                            >  SRR19973063.1224487/1‑150 (MQ=60)
                                                                                                                     ATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCT                           <  SRR19973063.2277128/150‑1 (MQ=60)
                                                                                                                      TTTCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTG                          <  SRR19973063.242372/150‑1 (MQ=60)
                                                                                                                        TCAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGT                        >  SRR19973063.3031128/1‑150 (MQ=60)
                                                                                                                         CAGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTC                       >  SRR19973063.3827972/1‑150 (MQ=60)
                                                                                                                          AGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCG                      >  SRR19973063.1917624/1‑150 (MQ=60)
                                                                                                                          AGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCG                      <  SRR19973063.4770710/150‑1 (MQ=60)
                                                                                                                          AGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCG                      >  SRR19973063.3377512/1‑150 (MQ=60)
                                                                                                                          AGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCG                      >  SRR19973063.3377472/1‑150 (MQ=60)
                                                                                                                          AGCCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCG                      <  SRR19973063.2894910/150‑1 (MQ=60)
                                                                                                                            CCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCC                    >  SRR19973063.3489394/1‑150 (MQ=60)
                                                                                                                            CCCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCC                    >  SRR19973063.3489495/1‑150 (MQ=60)
                                                                                                                             CCGGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCA                   >  SRR19973063.777154/1‑150 (MQ=60)
                                                                                                                               GGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATC                 <  SRR19973063.1486424/150‑1 (MQ=60)
                                                                                                                               GGCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATC                 >  SRR19973063.3210092/1‑150 (MQ=60)
                                                                                                                                GCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCT                >  SRR19973063.4730614/1‑150 (MQ=60)
                                                                                                                                GCAAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCT                <  SRR19973063.836160/150‑1 (MQ=60)
                                                                                                                                  AAATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGACTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTT              <  SRR19973063.3940710/150‑1 (MQ=60)
                                                                                                                                   AATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTC             <  SRR19973063.3696175/150‑1 (MQ=60)
                                                                                                                                   AATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTC             <  SRR19973063.1683985/150‑1 (MQ=60)
                                                                                                                                   AATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTC             <  SRR19973063.1516479/150‑1 (MQ=60)
                                                                                                                                   AATGCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTC             >  SRR19973063.419836/1‑150 (MQ=60)
                                                                                                                                      GCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCT          <  SRR19973063.4338492/150‑1 (MQ=60)
                                                                                                                                      GCCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCT          <  SRR19973063.359284/150‑1 (MQ=60)
                                                                                                                                       CCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTG         <  SRR19973063.1236644/150‑1 (MQ=60)
                                                                                                                                       CCAGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTG         >  SRR19973063.4481784/1‑150 (MQ=60)
                                                                                                                                         AGACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAA       >  SRR19973063.3698383/1‑150 (MQ=60)
                                                                                                                                           ACGGTTCCATTACTGGTTTTTTGAAGATTCAGAGTTGTTTGCCTGAATATTTTATTCACATTTACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCAAAGCTCTGGTCGCCATCTTTCGCTGAATT     >  SRR19973063.130117/1‑150 (MQ=60)
                                                                                                                                           ACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATT     <  SRR19973063.2649611/150‑1 (MQ=60)
                                                                                                                                           ACGGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATT     <  SRR19973063.3847993/150‑1 (MQ=60)
                                                                                                                                            CGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTA    >  SRR19973063.2968165/1‑150 (MQ=60)
                                                                                                                                             GGGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTAT   >  SRR19973063.4011045/1‑150 (MQ=60)
                                                                                                                                              GGTCCATTACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATC  <  SRR19973063.2542479/150‑1 (MQ=60)
                                                                                                                                              GGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATC  >  SRR19973063.265102/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                              
AGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCTTTCGCTGAATTATC  >  CP009273/1430724‑1431015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑