Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,305,116 C→A 100% intergenic (‑103/+9) apbE ← / ← ompC putative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,305,1160CA99.7% 686.9 / ‑6.0 205intergenic (‑103/+9)apbE/ompCputative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (90/114);  minor base T (0/1);  total (90/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 210 total aligned reads displayed.

GCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTA  >  CP009273/2304976‑2305260
                                                                                                                                            |                                                                                                                                                 
gCAGCCAGCTGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCgag                                                                                                                                            <  2:185054/148‑1 (MQ=255)
 cagccagcAGTGCCACGGGGTTAAAGCTTATTTCCATACGTTATTAACCTCATGCGAACGGTCGAAAGATTACACAAAAAAACTGATTTTGTACGCTGAAAAAAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCgaga                                                                                                                                           <  2:847790/148‑1 (MQ=255)
 cagccagcAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCgaga                                                                                                                                           <  2:2503149/148‑1 (MQ=255)
 cagccagcAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCgaga                                                                                                                                           <  2:2905021/148‑1 (MQ=255)
    ccagcAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTa                                                                                                                                        >  1:765476/1‑148 (MQ=255)
      agcagTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGa                                                                                                                                      <  2:1268460/148‑1 (MQ=255)
             ccACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTggca                                                                                                                               >  1:585106/1‑146 (MQ=255)
             ccACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTggca                                                                                                                               >  1:485083/1‑146 (MQ=255)
                cgcgGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAAc                                                                                                                            >  1:2463108/1‑148 (MQ=255)
                 gggggTAAAGCTTATTTCCATAGGTTATTATCCACTTGCGAACGGTCGCAAGAGTACACCAAAAAAGTGTGTTTGTACGCTGAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAAcc                                                                                                                           <  1:180641/146‑1 (MQ=255)
                   gggTAAAGCTTATTTCCATACGTGATGATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAg                                                                                                                         <  2:2154758/148‑1 (MQ=255)
                      tAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGAcc                                                                                                                      >  1:889166/1‑148 (MQ=255)
                       aaaGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGAccc                                                                                                                     >  2:2993900/1‑148 (MQ=255)
                        aaGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTGTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCa                                                                                                                    >  2:1105468/1‑148 (MQ=255)
                         aggttATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACAAAAAAACTGAGTTTGTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCag                                                                                                                   <  1:2905503/145‑1 (MQ=255)
                           cTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCagag                                                                                                                 <  2:650979/148‑1 (MQ=255)
                            ttATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGc                                                                                                                >  1:657419/1‑148 (MQ=255)
                            ttATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGc                                                                                                                <  2:3130938/148‑1 (MQ=255)
                               tttCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTAc                                                                                                             >  2:2691012/1‑148 (MQ=255)
                               tttCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTAc                                                                                                             >  1:918127/1‑148 (MQ=255)
                                ttCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACg                                                                                                            >  2:2675821/1‑148 (MQ=255)
                                ttCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACg                                                                                                            >  2:2531296/1‑148 (MQ=255)
                                ttCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACg                                                                                                            >  1:374852/1‑148 (MQ=255)
                                ttCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACg                                                                                                            >  2:2796395/1‑148 (MQ=255)
                                 tCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGa                                                                                                           >  1:1577698/1‑148 (MQ=255)
                                  ccATCCGGGATTATCCTCCTGCGAACGGTCGCAAGAGTACACCAAAAAACGGAGTTTGTACGCTGAAAACAATCAAACAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGAt                                                                                                          <  1:1305986/148‑1 (MQ=255)
                                  ccATACTTTATTACCCTCTGGCGACCGTCCGCAAGAGAACACAAAAAAACTGATTTTGTCCGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGAt                                                                                                          <  1:1455095/148‑1 (MQ=255)
                                  ccATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGAt                                                                                                          >  1:3057334/1‑148 (MQ=255)
                                  caaTACTTGATTATCCTCATGCGAACGGTCTAAAGATTACACCAAAAAACTGAGTTTGTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGAt                                                                                                          <  1:2047912/146‑1 (MQ=255)
                                    aTACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGt                                                                                                        >  1:81981/1‑148 (MQ=255)
                                    aTACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGAGGt                                                                                                        >  1:2067515/1‑148 (MQ=255)
                                     tACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGTCAAACCAGACCCAGAGCTACGATGtt                                                                                                       >  1:2694407/1‑148 (MQ=255)
                                      aCGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTa                                                                                                      >  2:3249158/1‑148 (MQ=255)
                                         tGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCa                                                                                                   >  1:3161455/1‑148 (MQ=255)
                                          gATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAg                                                                                                  >  2:1434076/1‑148 (MQ=255)
                                          gATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAg                                                                                                  >  1:2484654/1‑148 (MQ=255)
                                           aTTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAgt                                                                                                 <  2:2862385/148‑1 (MQ=255)
                                           aTTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAgt                                                                                                 <  2:2061234/148‑1 (MQ=255)
                                            ttATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAgtg                                                                                                <  2:782370/148‑1 (MQ=255)
                                                  tCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATg                                                                                          >  2:120365/1‑148 (MQ=255)
                                                  tCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATg                                                                                          <  1:1665155/148‑1 (MQ=255)
                                                  tCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATg                                                                                          >  2:2710194/1‑148 (MQ=255)
                                                  tCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATg                                                                                          <  1:2177218/148‑1 (MQ=255)
                                                   cATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGc                                                                                         <  1:1086409/148‑1 (MQ=255)
                                                   cATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGc                                                                                         <  1:1809502/148‑1 (MQ=255)
                                                          aCGGTCGCAAGAGGACACCAAAAAACGGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTc                                                                                  <  2:1774687/148‑1 (MQ=255)
                                                          aCGGTCGAAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGTCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTc                                                                                  <  2:101388/148‑1 (MQ=255)
                                                             gTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGTTGCCAGCGTCACg                                                                               >  1:679508/1‑148 (MQ=255)
                                                               cGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAg                                                                             >  2:1413528/1‑148 (MQ=255)
                                                               cGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAg                                                                             >  2:379134/1‑148 (MQ=255)
                                                                 cAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGGGTCACGAGTg                                                                           >  2:1930577/1‑148 (MQ=255)
                                                                   cgattaaacCAAAAACTTTATTTTTTACTCTTAAAAAAATTAAAAAAGGGCCCGCAGGCACTTTGTTAGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGaa                                                                         <  1:2006280/141‑1 (MQ=255)
                                                                     agTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACt                                                                       <  1:2123273/148‑1 (MQ=255)
                                                                       tACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTgg                                                                     >  2:2176885/1‑148 (MQ=255)
                                                                              aaaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgt‑cgt                                                             >  2:613005/1‑148 (MQ=255)
                                                                               acaaCTGAGTTTGTACGCTGAACACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAACTCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgt‑cgtc                                                            <  2:1252127/146‑1 (MQ=255)
                                                                               aaaaCTGAGTTTGTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgt‑cgtc                                                            <  1:1936328/148‑1 (MQ=255)
                                                                               aaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgt‑cgtc                                                            <  2:17034/148‑1 (MQ=255)
                                                                               aaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgt‑cgtc                                                            <  1:831956/148‑1 (MQ=255)
                                                                                aaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAACTCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTcc                                                           <  2:1053534/148‑1 (MQ=255)
                                                                                  aCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCcag                                                         >  1:2390032/1‑148 (MQ=255)
                                                                                    tGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCcagca                                                       >  1:2001463/1‑148 (MQ=255)
                                                                                     gagaTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCcagcag                                                      <  2:510747/148‑1 (MQ=255)
                                                                                      aGTTTGTACGCTGAAAACACTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAgg                                                     <  2:1731460/148‑1 (MQ=255)
                                                                                      aGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAgg                                                     <  2:1455415/148‑1 (MQ=255)
                                                                                      aGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAgg                                                     <  2:1457078/148‑1 (MQ=255)
                                                                                      aGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAgg                                                     <  2:56908/148‑1 (MQ=255)
                                                                                         ttGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTg                                                  <  2:2687582/148‑1 (MQ=255)
                                                                                            tACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGAtt                                               <  1:2410715/148‑1 (MQ=255)
                                                                                            tACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGAtt                                               <  1:1706251/148‑1 (MQ=255)
                                                                                                cTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGt                                           >  2:1509578/1‑148 (MQ=255)
                                                                                                cTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATAGATATTAGAACATGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACGGGTTGT‑CGTCCAGCAGGGTGATTTTGt                                           >  1:1492727/1‑148 (MQ=255)
                                                                                                  gAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAg                                         <  1:355978/148‑1 (MQ=255)
                                                                                                   aaaaCAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGt                                        >  1:1114010/1‑148 (MQ=255)
                                                                                                   aaaaCAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGt                                        >  1:2766165/1‑148 (MQ=255)
                                                                                                     aaCAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCa                                      >  1:2582000/1‑148 (MQ=255)
                                                                                                      aCAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCaa                                     >  2:2086748/1‑148 (MQ=255)
                                                                                                        aaTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACg                                   >  1:1503237/1‑148 (MQ=255)
                                                                                                        aaTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACg                                   <  1:996515/148‑1 (MQ=255)
                                                                                                            aaaaaaGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg                               <  2:713966/148‑1 (MQ=255)
                                                                                                               aaaGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTgg                            <  2:2463108/148‑1 (MQ=255)
                                                                                                                 aGGGCCCGCAGGCCCTGTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGAc                          >  1:1595450/1‑148 (MQ=255)
                                                                                                                    gCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATg                       <  1:185639/148‑1 (MQ=255)
                                                                                                                     cccGCAGGCCCATTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGt                      <  1:2860476/148‑1 (MQ=255)
                                                                                                                      ccGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtt                     <  1:2825598/148‑1 (MQ=255)
                                                                                                                         cAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttttt                  >  2:2480759/1‑148 (MQ=255)
                                                                                                                         cAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttttt                  >  2:1137025/1‑148 (MQ=255)
                                                                                                                           ggCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt                >  1:3186928/1‑148 (MQ=255)
                                                                                                                              ccTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGa             <  1:2301738/148‑1 (MQ=255)
                                                                                                                              ccTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGa             <  2:2287869/148‑1 (MQ=255)
                                                                                                                               cTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGaa            <  1:529397/148‑1 (MQ=255)
                                                                                                                                 ttGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagt          <  1:2952469/148‑1 (MQ=255)
                                                                                                                                    ttagATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt       <  2:889166/145‑1 (MQ=255)
                                                                                                                                    ttCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt       <  1:2796395/148‑1 (MQ=255)
                                                                                                                                     tCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAgtagta      <  1:1757371/148‑1 (MQ=255)
                                                                                                                                     tCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAgtagta      <  2:1860554/148‑1 (MQ=255)
                                                                                                                                     tCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAgtagta      <  1:1029421/148‑1 (MQ=255)
                                                                                                                                      cGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGtagtag     <  1:550008/148‑1 (MQ=255)
                                                                                                                                      cGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACT‑GTTGTGCGTCCAGCATGTTTATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGtagtag     <  1:3249158/148‑1 (MQ=37)
                                                                                                                                         tataAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggta  >  2:2258577/1‑148 (MQ=255)
                                                                                                                                            |                                                                                                                                                 
GCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTA  >  CP009273/2304976‑2305260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACC  >  CP009273/2304967‑2305265
                                                                                                                                                     |                                                                                                                                                      
AAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATA                                                                                                                                                        >  SRR19973070.436964/1‑150 (MQ=60)
          cccccagctggcccacggggttaacgtttattacaatacttgattaccctcaggccacgggtcgcatcataacacaaaaaacctgattttgaccgctcAAAACAATAAAAAACGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATT                                                                                                                                              <  SRR19973070.572176/52‑1 (MQ=49)
           AGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTA                                                                                                                                             >  SRR19973070.17285/1‑150 (MQ=60)
           AGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTA                                                                                                                                             >  SRR19973070.770854/1‑150 (MQ=60)
            GCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAGAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAG                                                                                                                                            <  SRR19973070.1403994/150‑1 (MQ=60)
                    TGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCA                                                                                                                                    >  SRR19973070.589139/1‑150 (MQ=60)
                    TGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCA                                                                                                                                    >  SRR19973070.488497/1‑150 (MQ=60)
                       CACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAAC                                                                                                                                 >  SRR19973070.2479188/1‑150 (MQ=60)
                       CACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAAC                                                                                                                                 >  SRR19973070.2481880/1‑150 (MQ=60)
                          GGGGGTAAAGCTTATTTCCATAGGTTATTATCCACTTGCGAACGGTCGCAAGAGTACACCAAAAAAGTGTGTTTGTACGCTGAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAG                                                                                                                              <  SRR19973070.182067/150‑1 (MQ=60)
                          GCGGTTAAAGATTATTTCAATACGTGTTTATCATCATGCGAAGGGTCGAAAGAGTACACCAAAAAACTTCGTTTGTACGCTGAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAG                                                                                                                              <  SRR19973070.2132674/150‑1 (MQ=60)
                           CGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAag                                                                                                                             >  SRR19973070.1939439/1‑148 (MQ=60)
                            GGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGAC                                                                                                                            >  SRR19973070.1543653/1‑150 (MQ=60)
                             GGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACC                                                                                                                           >  SRR19973070.895444/1‑150 (MQ=60)
                             GGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACC                                                                                                                           >  SRR19973070.896068/1‑150 (MQ=60)
                              GTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCC                                                                                                                          >  SRR19973070.1662335/1‑150 (MQ=60)
                                  AGGTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACAAAAAAACTGAGTTTGTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAG                                                                                                                      <  SRR19973070.2924294/150‑1 (MQ=60)
                                   tatttTTTCCATACTTTATTATCCTCATGCGAACGGTCGAAAGAGAACACAAAAAAACTTAGTTTGTACGCTCAAAAAAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGC                                                                                                                     <  SRR19973070.160568/145‑1 (MQ=60)
                                   GCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGC                                                                                                                     >  SRR19973070.662101/1‑150 (MQ=60)
                                   GCTTATTACCTTACGTTATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGATTTTGTACGCTTAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGC                                                                                                                     <  SRR19973070.1842593/150‑1 (MQ=60)
                                    ATTATTTCAATACGTGTTTATCCTCATGCGAACGGTCGCAAGAGTACACAAAAAAACTGAGTTTGTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCT                                                                                                                    <  SRR19973070.500337/150‑1 (MQ=60)
                                     TTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGA‑CCAGAGCAAC                                                                                                                  >  SRR19973070.2087769/1‑150 (MQ=60)
                                      TATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTAC                                                                                                                  >  SRR19973070.874559/1‑150 (MQ=60)
                                      TATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTAC                                                                                                                  >  SRR19973070.924665/1‑150 (MQ=60)
                                       ATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACG                                                                                                                 >  SRR19973070.377506/1‑150 (MQ=60)
                                       ATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACG                                                                                                                 >  SRR19973070.377368/1‑150 (MQ=60)
                                       ATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACG                                                                                                                 >  SRR19973070.377600/1‑150 (MQ=60)
                                        TTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGA                                                                                                                >  SRR19973070.1588765/1‑150 (MQ=60)
                                        TTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGA                                                                                                                >  SRR19973070.15234/1‑150 (MQ=60)
                                         ttcaatagttttttatcctctggctacggttctaataataaaaaaaaaaaattgtgttttaacgttaaaaaaaatcAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGAT                                                                                                               <  SRR19973070.21948/74‑1 (MQ=60)
                                         TTCCATACGTGATTATCCTCATGCGACCGGTCGCAATAGTACACCAAAAAACTGAGTTTGTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATTAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGAT                                                                                                               <  SRR19973070.2680536/150‑1 (MQ=60)
                                         TTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGAT                                                                                                               >  SRR19973070.3076874/1‑150 (MQ=60)
                                           ccatactttattaccctctggcgaccgtcCGCAAGAGAACACAAAAAAACTGATTTTGTCCGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGT                                                                                                             <  SRR19973070.1465234/121‑1 (MQ=60)
                                           CCATCCGGGATTATCCTCCTGCGAACGGTCGCAAGAGTACACCAAAAAACGGAGTTTGTACGCTGAAAACAATCAAACAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGT                                                                                                             <  SRR19973070.1315059/150‑1 (MQ=60)
                                           CCATACTTGATTATCCTCATGCGAACGGTCGCAAGAGTACACAAAAAAACTGAGTTTGTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGT                                                                                                             <  SRR19973070.2061786/150‑1 (MQ=60)
                                           CCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGT                                                                                                             >  SRR19973070.82930/1‑150 (MQ=60)
                                           CCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGT                                                                                                             >  SRR19973070.82833/1‑150 (MQ=60)
                                           CCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGAGGT                                                                                                             >  SRR19973070.2081450/1‑150 (MQ=60)
                                           CAATACTTGATTATCCTCATGCGAACGGTCTAAAGATTACACCAAAAAACTGAGTTTGTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGT                                                                                                             <  SRR19973070.2061765/150‑1 (MQ=60)
                                            aaacctttttaatcctcatgccaaggtgccaatgagaacacaaaaaacgtttttttttagcctaaaaaaaatcAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTT                                                                                                            <  SRR19973070.2267003/77‑1 (MQ=60)
                                            CATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGTCAAACCAGACCCAGAGCTACGATGTT                                                                                                            >  SRR19973070.2711860/1‑150 (MQ=60)
                                                cttgagtatcatctcgcgaacggtcgaaagaaaacaaaataaaaattattttgtcgggttaaaaatttaAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCA                                                                                                        <  SRR19973070.2122515/81‑1 (MQ=60)
                                                CGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCA                                                                                                        >  SRR19973070.3181913/1‑150 (MQ=60)
                                                CGTGATTAACCTCATGCGAACGGTCGCACGAGTACACAAAAAAAGTGAGTTTGTCCGCTCAAAAAAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCA                                                                                                        <  SRR19973070.3212950/150‑1 (MQ=60)
                                                 GTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCCGAGCTACGATGTTATCAG                                                                                                       >  SRR19973070.2500808/1‑150 (MQ=60)
                                                 GTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAG                                                                                                       >  SRR19973070.854068/1‑150 (MQ=60)
                                                           TCATGCGACCGGCCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTCAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCC                                                                                             <  SRR19973070.2852757/150‑1 (MQ=60)
                                                           TCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCC                                                                                             <  SRR19973070.1676600/150‑1 (MQ=60)
                                                           TCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCC                                                                                             <  SRR19973070.2191636/150‑1 (MQ=60)
                                                           CCATGCGAACGGTCGCACGATAACACCAAAAAACTGCGTTTGTACGCTCAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCC                                                                                             <  SRR19973070.1194245/150‑1 (MQ=60)
                                                            CATGCGACCGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCA                                                                                            <  SRR19973070.1821940/150‑1 (MQ=60)
                                                            CATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCA                                                                                            <  SRR19973070.1093941/150‑1 (MQ=60)
                                                            CATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCA                                                                                            <  SRR19973070.1821980/150‑1 (MQ=60)
                                                                 GAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTC                                                                                       >  SRR19973070.2975551/1‑150 (MQ=60)
                                                                    CGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGTTGCCAGCGTCACG                                                                                    >  SRR19973070.684351/1‑150 (MQ=60)
                                                                    CGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACG                                                                                    <  SRR19973070.2465388/150‑1 (MQ=60)
                                                                     GGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGA                                                                                   >  SRR19973070.64829/1‑150 (MQ=60)
                                                                            cgattaaaccaaaaactttattttttactcttaaaaaaattAAAAAAGGGCCCGCAGGCACTTTGTTAGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACT                                                                            <  SRR19973070.2019865/109‑1 (MQ=60)
                                                                            AGAGAACACCAAAAAACTTAGTTTGTCCGCTCAAAAAAAGGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACT                                                                            <  SRR19973070.2019789/150‑1 (MQ=60)
                                                                            AGAGAACACCAAAAAACTGATTTTTTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACT                                                                            <  SRR19973070.2019872/150‑1 (MQ=60)
                                                                              CGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGG                                                                          <  SRR19973070.2137483/150‑1 (MQ=60)
                                                                                  CACCAAAAAACTGAGTTTGTCCGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCAAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT                                                                      <  SRR19973070.2022462/150‑1 (MQ=60)
                                                                                        AAAACTGAGTTTGTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCA                                                               <  SRR19973070.1949347/150‑1 (MQ=60)
                                                                                        AAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCA                                                               <  SRR19973070.837597/150‑1 (MQ=60)
                                                                                         AAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAG                                                              >  SRR19973070.2405812/1‑150 (MQ=60)
                                                                                         AAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAG                                                              >  SRR19973070.2383354/1‑150 (MQ=60)
                                                                                           ACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCA                                                            >  SRR19973070.2015015/1‑150 (MQ=60)
                                                                                           ACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCA                                                            >  SRR19973070.337260/1‑150 (MQ=60)
                                                                                                GTTTGTACGCTCAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTG                                                       <  SRR19973070.2810542/150‑1 (MQ=60)
                                                                                                    GTACGCTCAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTT                                                   <  SRR19973070.3092153/150‑1 (MQ=60)
                                                                                                     TACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTT                                                  <  SRR19973070.2426598/150‑1 (MQ=60)
                                                                                                     TACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTT                                                  <  SRR19973070.1718027/150‑1 (MQ=60)
                                                                                                     TACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTT                                                  <  SRR19973070.2426581/150‑1 (MQ=60)
                                                                                                     TACGCTCAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTT                                                  <  SRR19973070.1718021/150‑1 (MQ=60)
                                                                                                       CGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGT                                                >  SRR19973070.1502004/1‑150 (MQ=60)
                                                                                                       CGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATAGATATTAGAACATGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACGGGTTGT‑CGTCCAGCAGGGTGATTTTGT                                                >  SRR19973070.1503171/1‑150 (MQ=60)
                                                                                                          TGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGT                                             >  SRR19973070.2783882/1‑150 (MQ=60)
                                                                                                          TGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGT                                             >  SRR19973070.2783901/1‑150 (MQ=60)
                                                                                                          TGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGT                                             >  SRR19973070.1121622/1‑150 (MQ=60)
                                                                                                           TAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTC                                            <  SRR19973070.358570/150‑1 (MQ=60)
                                                                                                            AAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCA                                           <  SRR19973070.1764808/150‑1 (MQ=60)
                                                                                                            AAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCA                                           >  SRR19973070.2598877/1‑150 (MQ=60)
                                                                                                              AACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAAC                                         <  SRR19973070.2563799/150‑1 (MQ=60)
                                                                                                              AACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAAC                                         >  SRR19973070.2172252/1‑150 (MQ=60)
                                                                                                              AACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAAC                                         >  SRR19973070.1111254/1‑150 (MQ=60)
                                                                                                               ACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACG                                        >  SRR19973070.1513760/1‑150 (MQ=60)
                                                                                                                 AATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTA                                      >  SRR19973070.477788/1‑150 (MQ=60)
                                                                                                                 AATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTA                                      <  SRR19973070.278312/150‑1 (MQ=60)
                                                                                                                 AATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTA                                      <  SRR19973070.1003620/150‑1 (MQ=60)
                                                                                                                 AATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTA                                      <  SRR19973070.1004812/150‑1 (MQ=60)
                                                                                                                      AAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGG                                 >  SRR19973070.948165/1‑150 (MQ=60)
                                                                                                                        AAAGGGCCCGCAGGCCCTGTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGAC                               >  SRR19973070.1606600/1‑150 (MQ=60)
                                                                                                                            GGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGT                           >  SRR19973070.3199951/1‑150 (MQ=60)
                                                                                                                             GCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTT                          <  SRR19973070.187087/150‑1 (MQ=60)
                                                                                                                             GCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTT                          <  SRR19973070.1974807/150‑1 (MQ=60)
                                                                                                                              CCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTT                         <  SRR19973070.2879256/150‑1 (MQ=60)
                                                                                                                              CCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTT                         >  SRR19973070.675012/1‑150 (MQ=60)
                                                                                                                              CCCGCAGGCCCATTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTT                         <  SRR19973070.2879014/150‑1 (MQ=60)
                                                                                                                               CCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTT                        <  SRR19973070.2843668/150‑1 (MQ=60)
                                                                                                                               CCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTT                        <  SRR19973070.2843882/150‑1 (MQ=60)
                                                                                                                                  CAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGT                     >  SRR19973070.3207548/1‑150 (MQ=60)
                                                                                                                                    GGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTG                   >  SRR19973070.742865/1‑150 (MQ=60)
                                                                                                                                      CCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAA                 >  SRR19973070.802197/1‑150 (MQ=60)
                                                                                                                                       CCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAG                <  SRR19973070.2317048/150‑1 (MQ=60)
                                                                                                                                        CTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGT               <  SRR19973070.533018/150‑1 (MQ=60)
                                                                                                                                        CTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGT               <  SRR19973070.532974/150‑1 (MQ=60)
                                                                                                                                          TTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAG             <  SRR19973070.2971471/150‑1 (MQ=60)
                                                                                                                                          TTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAG             <  SRR19973070.2971470/150‑1 (MQ=60)
                                                                                                                                             TTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAG          <  SRR19973070.2814438/150‑1 (MQ=60)
                                                                                                                                              TCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGG         <  SRR19973070.1036711/150‑1 (MQ=60)
                                                                                                                                              TCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGG         <  SRR19973070.1769543/150‑1 (MQ=60)
                                                                                                                                               CGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGT        <  SRR19973070.553661/150‑1 (MQ=60)
                                                                                                                                               CGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACT‑GTTGTGCGTCCAGCATGTTTATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGT        <  SRR19973070.3270094/150‑1 (MQ=60)
                                                                                                                                                GATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTA       >  SRR19973070.1797083/1‑150 (MQ=60)
                                                                                                                                                GATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTA       >  SRR19973070.2206150/1‑150 (MQ=60)
                                                                                                                                                 ATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAG      <  SRR19973070.1060957/150‑1 (MQ=60)
                                                                                                                                                 ATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAG      <  SRR19973070.578260/150‑1 (MQ=60)
                                                                                                                                                   ATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCA    <  SRR19973070.2530776/150‑1 (MQ=60)
                                                                                                                                                     AAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACC  >  SRR19973070.1275871/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                      
AAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGT‑CGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACC  >  CP009273/2304967‑2305265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑