Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,305,116 C→A 91.4% intergenic (‑103/+9) apbE ← / ← ompC putative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,305,1160CA91.4% 735.7 / 52.6 238intergenic (‑103/+9)apbE/ompCputative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base C (7/14);  new base A (90/126);  total (97/141)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 239 total aligned reads displayed.

AGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAC  >  CP009273/2304970‑2305264
                                                                                                                                                  |                                                                                                                                                    
     ggCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCgaga                                                                                                                                              <  2:3687587/150‑1 (MQ=255)
       cagccagcAGTGCCACGCGGGTAAAGCTTATTTCCATACGTTATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTCCGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGAtt                                                                                                                                            <  1:4528777/150‑1 (MQ=255)
            agcagTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGACCGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAAc                                                                                                                                       <  2:672260/150‑1 (MQ=255)
                  gCCTCGCGGGTAAATTTTATTTCCATACGTGATTATCATCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGTGTTTTTACGCTGAAAAAAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCaa                                                                                                                                 <  1:2269953/150‑1 (MQ=255)
                  gCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCGGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTaa                                                                                                                                 <  2:2817985/150‑1 (MQ=255)
                   ccACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCaaa                                                                                                                                >  1:4869047/1‑150 (MQ=255)
                            tAAAGCTTATTTCAATCCGTGATTATCCTAATGCGAACGGTCGCAATATTACACAAAAAAACTGATTTTTTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCa                                                                                                                       <  1:3076459/150‑1 (MQ=255)
                                gCTTATTTCCATACGTGTTTATCCTCATGCGACCGGTCGCAAGAGTACACCAAAAAACTGTGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGc                                                                                                                   <  1:2099511/150‑1 (MQ=255)
                                gCTTATCTCCATACGTGATTATCCTCATGCGAACGTTCGCAAGAGTACACCAAAAAACTGTGTTTTTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGc                                                                                                                   <  1:145144/150‑1 (MQ=255)
                                 cTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCt                                                                                                                  <  2:5109688/150‑1 (MQ=255)
                                   tATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTAc                                                                                                                >  2:3516880/1‑150 (MQ=255)
                                   tATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACAAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTAc                                                                                                                <  1:1623378/150‑1 (MQ=255)
                                     tttCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAATAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGa                                                                                                              >  1:962691/1‑150 (MQ=255)
                                      ttCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGAt                                                                                                             >  1:3126598/1‑150 (MQ=255)
                                      ttCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGAt                                                                                                             <  2:1042757/150‑1 (MQ=255)
                                        ccATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGt                                                                                                           <  2:2130539/150‑1 (MQ=255)
                                         cATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGtt                                                                                                          <  2:1474580/150‑1 (MQ=255)
                                              gTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAg                                                                                                     >  1:13732/1‑150 (MQ=255)
                                              gTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAg                                                                                                     >  1:4986096/1‑150 (MQ=255)
                                                 aTTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCCGACCCAGAGCTACGATGTTATCAgtgt                                                                                                  >  2:1846334/1‑150 (MQ=255)
                                                  ttATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGtt                                                                                                 >  1:5111813/1‑150 (MQ=255)
                                                   tATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTg                                                                                                <  1:4809456/150‑1 (MQ=255)
                                                    aTCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGa                                                                                               <  1:1255099/150‑1 (MQ=255)
                                                     tCCTCATGCGATCGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGGAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAt                                                                                              <  2:2713566/150‑1 (MQ=255)
                                                     tCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAt                                                                                              <  1:3539860/150‑1 (MQ=255)
                                                     tCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAt                                                                                              <  2:5170882/150‑1 (MQ=255)
                                                     tCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAt                                                                                              <  1:3247071/150‑1 (MQ=255)
                                                     gccTCAGGCGAACGGTCGAAAGAGTACACCAAAAAACTGTGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAt                                                                                              <  1:1001628/149‑1 (MQ=255)
                                                      ccacATGCGAACGGTCGCAAGAGAACACCAAAAAACTGCGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATg                                                                                             <  1:3495624/147‑1 (MQ=255)
                                                         cATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCa                                                                                          <  2:3895827/150‑1 (MQ=255)
                                                          aTGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAg                                                                                         <  2:5040312/150‑1 (MQ=255)
                                                          aTGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAg                                                                                         <  2:5040227/150‑1 (MQ=255)
                                                           tGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGc                                                                                        >  1:1056195/1‑150 (MQ=255)
                                                            gCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCg                                                                                       <  2:1182060/150‑1 (MQ=255)
                                                             cGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTCCGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGt                                                                                      <  1:1965180/150‑1 (MQ=255)
                                                                   gTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAg                                                                                >  1:2470090/1‑150 (MQ=255)
                                                                   gTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGG‑CAACCAGACCCAGAGCTACGATGTTATCCGTGTTGATGCCAGCGTCACGAGt                                                                               >  1:2518664/1‑150 (MQ=255)
                                                                      gCAAGAGTACACCAAAACACTGAGTGTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGa                                                                             <  2:3540720/150‑1 (MQ=255)
                                                                      gCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGa                                                                             >  2:1391283/1‑150 (MQ=255)
                                                                        aaGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAAc                                                                           <  2:4238235/150‑1 (MQ=255)
                                                                        aaGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAAc                                                                           >  2:1941135/1‑150 (MQ=255)
                                                                         agagTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACt                                                                          <  1:3826925/150‑1 (MQ=255)
                                                                         agagTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACt                                                                          <  1:318335/150‑1 (MQ=255)
                                                                               cacCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgt                                                                    >  2:3610607/1‑150 (MQ=255)
                                                                                acCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtc                                                                   >  1:4278742/1‑150 (MQ=255)
                                                                                   aaaaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcgtc                                                                >  2:4977104/1‑150 (MQ=255)
                                                                                    aaaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTcc                                                               <  1:3706006/150‑1 (MQ=255)
                                                                                           gAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGt                                                        <  1:3373353/150‑1 (MQ=255)
                                                                                           gAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGt                                                        <  1:2618438/150‑1 (MQ=255)
                                                                                           gAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTGTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAggg                                                        >  1:4753627/1‑149 (MQ=255)
                                                                                           gAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGACCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGt                                                        >  1:4753789/1‑150 (MQ=255)
                                                                                            aGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGTCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGtt                                                       >  1:5092985/1‑150 (MQ=255)
                                                                                              tttGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGa                                                     >  1:2006410/1‑150 (MQ=255)
                                                                                                  tACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTGGTCGTCCAGCAGGTTGAtttt                                                 >  2:3417630/1‑150 (MQ=255)
                                                                                                  tACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCGGTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAtttt                                                 >  1:840515/1‑150 (MQ=255)
                                                                                                     gCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTa                                              >  1:3522112/1‑150 (MQ=255)
                                                                                                      cTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAg                                             <  1:3998424/150‑1 (MQ=255)
                                                                                                       tGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAGTTTGTAGt                                            >  1:4230261/1‑150 (MQ=255)
                                                                                                       tGAAAACAATGAAAAAAGGGCCCGCAGGCCCTGTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGGGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAGTGTGTAGt                                            >  2:3457628/1‑150 (MQ=255)
                                                                                                        gAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTTGTAAACCAGACCCAGAGCTACGATGTTTTCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTc                                           <  1:3748022/150‑1 (MQ=255)
                                                                                                        gAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTc                                           >  2:4700751/1‑150 (MQ=255)
                                                                                                           aaCAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAAc                                        <  1:185935/150‑1 (MQ=255)
                                                                                                            aCAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATTAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACg                                       <  2:1442123/150‑1 (MQ=255)
                                                                                                            aCAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACg                                       <  1:3065542/150‑1 (MQ=255)
                                                                                                            aCAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACg                                       <  1:3065601/150‑1 (MQ=255)
                                                                                                             cAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt                                      <  2:2151688/150‑1 (MQ=255)
                                                                                                              aaTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTa                                     >  1:2849254/1‑150 (MQ=255)
                                                                                                               aTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAg                                    <  2:33079/150‑1 (MQ=255)
                                                                                                                  aaaaaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTg                                 <  2:4614528/150‑1 (MQ=255)
                                                                                                                     aaaGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGAc                              >  2:738973/1‑150 (MQ=255)
                                                                                                                      aaGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACa                             <  2:882489/150‑1 (MQ=255)
                                                                                                                       aGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt                            >  1:1226069/1‑150 (MQ=255)
                                                                                                                       aGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt                            <  1:1179432/150‑1 (MQ=255)
                                                                                                                        gggCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATg                           >  1:2243207/1‑150 (MQ=255)
                                                                                                                           cccGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttt                        <  2:4725093/150‑1 (MQ=255)
                                                                                                                             cGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttttt                      <  2:663898/150‑1 (MQ=255)
                                                                                                                             cGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttttt                      >  2:3648314/1‑150 (MQ=255)
                                                                                                                             cGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttttt                      >  2:1892640/1‑150 (MQ=255)
                                                                                                                             cGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttttt                      >  2:3883374/1‑150 (MQ=255)
                                                                                                                             cGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttttt                      >  1:3686257/1‑150 (MQ=255)
                                                                                                                                aGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgtt                   <  2:4372590/150‑1 (MQ=255)
                                                                                                                                aGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgtt                   <  1:2082726/150‑1 (MQ=255)
                                                                                                                                aGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgtt                   >  1:2895113/1‑150 (MQ=255)
                                                                                                                                   cccTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGaa                <  2:1325717/150‑1 (MQ=255)
                                                                                                                                    ccTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag               <  2:1737453/150‑1 (MQ=255)
                                                                                                                                    ccTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag               <  1:1391283/150‑1 (MQ=255)
                                                                                                                                    ccTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag               <  1:3433015/150‑1 (MQ=255)
                                                                                                                                    ccTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTGTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag               >  2:4272817/1‑150 (MQ=255)
                                                                                                                                     cTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagt              <  1:1600690/150‑1 (MQ=255)
                                                                                                                                      tttGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagta             >  1:1656893/1‑150 (MQ=255)
                                                                                                                                      tttGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagta             >  1:2128477/1‑150 (MQ=255)
                                                                                                                                      tttGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagta             <  2:2428901/150‑1 (MQ=255)
                                                                                                                                      tttGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagta             >  2:1006172/1‑150 (MQ=255)
                                                                                                                                        tGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt           >  2:795824/1‑150 (MQ=255)
                                                                                                                                        agTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTCATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCCGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt           >  2:4771690/2‑150 (MQ=255)
                                                                                                                                         gTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAgtagta          >  2:4626354/1‑150 (MQ=255)
                                                                                                                                            cGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggt       <  2:3009668/150‑1 (MQ=255)
                                                                                                                                            cGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggt       >  2:2646576/1‑150 (MQ=255)
                                                                                                                                               tataAATCGAGATTAGAACTGGCAAACCGGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGc    <  1:1091033/150‑1 (MQ=255)
                                                                                                                                                 tCATTCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAc  <  1:1715481/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAC  >  CP009273/2304970‑2305264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACC  >  CP009273/2304975‑2305265
                                                                                                                                             |                                                                                                                                                     
GGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGA                                                                                                                                               >  SRR19973083.1023895/1‑150 (MQ=60)
GGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGA                                                                                                                                               >  SRR19973083.2369091/1‑150 (MQ=60)
  CAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTTATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTCCGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATT                                                                                                                                             <  SRR19973083.4548423/150‑1 (MQ=60)
        GCTGTGCCACGCGGTTAAATATTTTTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGTGAAAACAAAAAAACTGAGTTTTTACGCTGAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACT                                                                                                                                       <  SRR19973083.967987/150‑1 (MQ=60)
             GCCTCGCGGGTAAATTTTATTTCCATACGTGATTATCATCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGTGTTTTTACGCTGAAAAAAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAA                                                                                                                                  <  SRR19973083.2281554/150‑1 (MQ=60)
             GCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCTTGCGAACGGTCGCAAGAGTACACAAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAA                                                                                                                                  <  SRR19973083.3739659/150‑1 (MQ=60)
              CCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAA                                                                                                                                 >  SRR19973083.4889998/1‑150 (MQ=60)
              CCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAA                                                                                                                                 >  SRR19973083.4889953/1‑150 (MQ=60)
               CACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAAC                                                                                                                                >  SRR19973083.4996449/1‑150 (MQ=60)
               CACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAAC                                                                                                                                >  SRR19973083.3484901/1‑150 (MQ=60)
                   CGGTTAAAGATTATTTCAATCCGTGATTATCCTCATGCTAACGGTCGCACGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGA                                                                                                                            <  SRR19973083.226066/150‑1 (MQ=60)
                       TAAAGCTTATTTCCATACGTGATTATCCTCATGCGACGGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCA                                                                                                                        <  SRR19973083.3091181/150‑1 (MQ=60)
                       TAAAGCTTATTTCAATCCGTGATTATCCTAATGCGAACGGTCGCAATATTACACAAAAAAACTGATTTTTTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCA                                                                                                                        <  SRR19973083.3091207/150‑1 (MQ=60)
                           GCTTATTTCCATACGTGTTTATCCTCATGCGACCGGTCGCAAGAGTACACCAAAAAACTGTGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGC                                                                                                                    <  SRR19973083.2110149/150‑1 (MQ=60)
                           GCTTATCTCCATACGTGATTATCCTCATGCGAACGTTCGCAAGAGTACACCAAAAAACTGTGTTTTTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGC                                                                                                                    <  SRR19973083.146023/150‑1 (MQ=60)
                             TTATTTCAATACGTGATTATCCTCATGCTCACGGTCGCAAGAGTACACCAACAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTA                                                                                                                  <  SRR19973083.4586429/150‑1 (MQ=60)
                              TATTTCCATACTTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGATTTTGTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTAC                                                                                                                 <  SRR19973083.294345/150‑1 (MQ=60)
                              TATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTAC                                                                                                                 <  SRR19973083.294379/150‑1 (MQ=60)
                              TATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACAAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTAC                                                                                                                 <  SRR19973083.1631483/150‑1 (MQ=60)
                               ATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACG                                                                                                                >  SRR19973083.109867/1‑150 (MQ=60)
                                TTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAATAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGA                                                                                                               >  SRR19973083.967448/1‑150 (MQ=60)
                                 TTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGAT                                                                                                              >  SRR19973083.3141553/1‑150 (MQ=60)
                                       ACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATC                                                                                                        <  SRR19973083.1616673/150‑1 (MQ=60)
                                        CGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCA                                                                                                       <  SRR19973083.4781648/150‑1 (MQ=60)
                                        CGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCA                                                                                                       <  SRR19973083.4782241/150‑1 (MQ=60)
                                         GTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAG                                                                                                      >  SRR19973083.3609570/1‑150 (MQ=60)
                                         GTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAG                                                                                                      >  SRR19973083.13848/1‑150 (MQ=60)
                                         GTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAG                                                                                                      >  SRR19973083.5007505/1‑150 (MQ=60)
                                           GATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGAGGTTATCAGTG                                                                                                    >  SRR19973083.1092666/1‑150 (MQ=60)
                                             TTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTT                                                                                                  >  SRR19973083.5133601/1‑150 (MQ=60)
                                              TATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTG                                                                                                 <  SRR19973083.4830056/150‑1 (MQ=60)
                                               ATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGA                                                                                                <  SRR19973083.1261220/150‑1 (MQ=60)
                                               ATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGA                                                                                                >  SRR19973083.1418831/1‑150 (MQ=60)
                                                gcctaATGCGACCGGTCGCAACAGTACACCAAAAAACTGTTTTTGTACGCTCAAAAAAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAT                                                                                               <  SRR19973083.914899/145‑1 (MQ=60)
                                                TCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAT                                                                                               >  SRR19973083.2742990/1‑150 (MQ=60)
                                                TCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAT                                                                                               <  SRR19973083.3556115/150‑1 (MQ=60)
                                                TCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAT                                                                                               <  SRR19973083.3262425/150‑1 (MQ=60)
                                                TCCTCATGCGAACGGTCGCAAGAGCACAAAAAAAAACTTAGTTTGTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAT                                                                                               <  SRR19973083.3530190/150‑1 (MQ=60)
                                                GCCTCATGCTAACGGTCCCAAGAGTACACAAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAT                                                                                               <  SRR19973083.3556101/150‑1 (MQ=60)
                                                GCCTCAGGCGAACGGTCGAAAGAGTACACCAAAAAACTGTGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAT                                                                                               <  SRR19973083.1006644/150‑1 (MQ=60)
                                                 CCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATG                                                                                              <  SRR19973083.3511315/150‑1 (MQ=60)
                                                 CCACATGCGAACGGTCGCAAGAGAACACCAAAAAACTGCGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATG                                                                                              <  SRR19973083.3511637/150‑1 (MQ=60)
                                                     ATGCGAACGGTCGCAAGAGTACCCCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAG                                                                                          <  SRR19973083.4122508/150‑1 (MQ=60)
                                                      TGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGC                                                                                         >  SRR19973083.1061513/1‑150 (MQ=60)
                                                        CGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTCCGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGT                                                                                       <  SRR19973083.1975223/150‑1 (MQ=60)
                                                        CGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGT                                                                                       >  SRR19973083.3260284/1‑150 (MQ=60)
                                                         GAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTC                                                                                      >  SRR19973083.2327303/1‑150 (MQ=60)
                                                              GTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAG                                                                                 >  SRR19973083.2482731/1‑150 (MQ=60)
                                                              GTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGG‑CAACCAGACCCAGAGCTACGATGTTATCCGTGTTGATGCCAGCGTCACGAGT                                                                                >  SRR19973083.2531466/1‑150 (MQ=60)
                                                                    AGATTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCATTTGTTCGATATACATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACT                                                                           <  SRR19973083.319943/150‑1 (MQ=60)
                                                                    AGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACT                                                                           <  SRR19973083.319939/150‑1 (MQ=60)
                                                                    AGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACT                                                                           <  SRR19973083.3844109/150‑1 (MQ=60)
                                                                      AGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGG                                                                         <  SRR19973083.4279433/150‑1 (MQ=60)
                                                                      AGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGG                                                                         <  SRR19973083.1184351/150‑1 (MQ=60)
                                                                      AGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGG                                                                         <  SRR19973083.1676232/150‑1 (MQ=60)
                                                                       GTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGT                                                                        >  SRR19973083.2191262/1‑150 (MQ=60)
                                                                           ACCAAAAAACTGTGTTTGTACGCTGAAACCAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTC                                                                    <  SRR19973083.1851064/150‑1 (MQ=60)
                                                                           ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTC                                                                    <  SRR19973083.389142/150‑1 (MQ=60)
                                                                           ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTC                                                                    >  SRR19973083.4297395/1‑150 (MQ=60)
                                                                           ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTC                                                                    <  SRR19973083.373133/150‑1 (MQ=60)
                                                                           ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTC                                                                    >  SRR19973083.3115606/1‑150 (MQ=60)
                                                                           ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTC                                                                    >  SRR19973083.3115867/1‑150 (MQ=60)
                                                                           ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTGTGTTCGATATAAATCGAGAGTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAAGTGGTTGTC                                                                    >  SRR19973083.1302793/1‑150 (MQ=60)
                                                                           ACAAAAAAACTGAGTTTGTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTC                                                                    <  SRR19973083.522401/150‑1 (MQ=60)
                                                                           ACAAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTC                                                                    <  SRR19973083.1410812/150‑1 (MQ=60)
                                                                              aaaaatctttttttgtccgtttAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTC                                                                 <  SRR19973083.3077513/128‑1 (MQ=60)
                                                                               AAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCC                                                                <  SRR19973083.3722880/150‑1 (MQ=60)
                                                                               AAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCC                                                                <  SRR19973083.3722866/150‑1 (MQ=60)
                                                                                    CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAG                                                           >  SRR19973083.4082481/1‑150 (MQ=60)
                                                                                      GAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGT                                                         <  SRR19973083.1484745/150‑1 (MQ=60)
                                                                                      GAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGT                                                         <  SRR19973083.2631583/150‑1 (MQ=60)
                                                                                      GAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGT                                                         <  SRR19973083.2632009/150‑1 (MQ=60)
                                                                                      GAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGT                                                         <  SRR19973083.3389017/150‑1 (MQ=60)
                                                                                      GAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGT                                                         >  SRR19973083.4773943/1‑150 (MQ=60)
                                                                                      GAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTGTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGG                                                         >  SRR19973083.4773906/1‑150 (MQ=60)
                                                                                      GAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGACCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGT                                                         >  SRR19973083.4774068/1‑150 (MQ=60)
                                                                                       AGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGTCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTT                                                        >  SRR19973083.5114738/1‑150 (MQ=60)
                                                                                       AGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTT                                                        >  SRR19973083.5115059/1‑150 (MQ=60)
                                                                                         TTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGA                                                      >  SRR19973083.2016650/1‑150 (MQ=60)
                                                                                             TACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTT                                                  >  SRR19973083.844482/1‑150 (MQ=60)
                                                                                             TACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCGGTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTT                                                  >  SRR19973083.844489/1‑150 (MQ=60)
                                                                                              ACGCTCAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTG                                                 <  SRR19973083.2514158/150‑1 (MQ=60)
                                                                                                GCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTA                                               >  SRR19973083.3538264/1‑150 (MQ=60)
                                                                                                 CTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAG                                              <  SRR19973083.4016208/150‑1 (MQ=60)
                                                                                                  TGAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGT                                             <  SRR19973083.2563567/150‑1 (MQ=60)
                                                                                                  TGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGT                                             <  SRR19973083.1296199/150‑1 (MQ=60)
                                                                                                  TGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAGTTTGTAGT                                             >  SRR19973083.4248792/1‑150 (MQ=60)
                                                                                                   GAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTTGTAAACCAGACCCAGAGCTACGATGTTTTCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTC                                            <  SRR19973083.3765007/150‑1 (MQ=60)
                                                                                                    AAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCA                                           <  SRR19973083.1951052/150‑1 (MQ=60)
                                                                                                      AACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAAC                                         <  SRR19973083.186986/150‑1 (MQ=60)
                                                                                                       ACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACG                                        <  SRR19973083.3080238/150‑1 (MQ=60)
                                                                                                       ACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACG                                        <  SRR19973083.3080246/150‑1 (MQ=60)
                                                                                                       ACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACG                                        <  SRR19973083.3080297/150‑1 (MQ=60)
                                                                                                        CAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGT                                       >  SRR19973083.3543544/1‑150 (MQ=60)
                                                                                                        CAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGT                                       <  SRR19973083.1218649/150‑1 (MQ=60)
                                                                                                         AATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTA                                      >  SRR19973083.2863324/1‑150 (MQ=60)
                                                                                                           TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGG                                    <  SRR19973083.4432858/150‑1 (MQ=60)
                                                                                                           TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGG                                    <  SRR19973083.4432887/150‑1 (MQ=60)
                                                                                                               AAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGA                                >  SRR19973083.326659/1‑150 (MQ=60)
                                                                                                               AAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGA                                >  SRR19973083.3429364/1‑150 (MQ=60)
                                                                                                                AAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGAC                               <  SRR19973083.1812952/150‑1 (MQ=60)
                                                                                                                  AGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT                             >  SRR19973083.1232088/1‑150 (MQ=60)
                                                                                                                  AGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT                             >  SRR19973083.1232589/1‑150 (MQ=60)
                                                                                                                  AGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT                             >  SRR19973083.4848963/1‑150 (MQ=60)
                                                                                                                  AGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT                             <  SRR19973083.1185285/150‑1 (MQ=60)
                                                                                                                   GGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATG                            >  SRR19973083.2254601/1‑150 (MQ=60)
                                                                                                                     GCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTT                          <  SRR19973083.3829823/150‑1 (MQ=60)
                                                                                                                        CGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTT                       >  SRR19973083.3703067/1‑150 (MQ=60)
                                                                                                                        CGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTT                       <  SRR19973083.1984511/150‑1 (MQ=60)
                                                                                                                         GCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTG                      <  SRR19973083.2098303/150‑1 (MQ=60)
                                                                                                                          CAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGT                     >  SRR19973083.3150046/1‑150 (MQ=60)
                                                                                                                           AGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTT                    <  SRR19973083.2092952/150‑1 (MQ=60)
                                                                                                                           AGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTT                    <  SRR19973083.2093008/150‑1 (MQ=60)
                                                                                                                           AGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTT                    <  SRR19973083.2093306/150‑1 (MQ=60)
                                                                                                                           AGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTT                    >  SRR19973083.2909305/1‑150 (MQ=60)
                                                                                                                           AGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTT                    >  SRR19973083.2909224/1‑150 (MQ=60)
                                                                                                                            GGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTG                   <  SRR19973083.2127409/150‑1 (MQ=60)
                                                                                                                               CCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAG                <  SRR19973083.1397987/150‑1 (MQ=60)
                                                                                                                               CCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAG                <  SRR19973083.3448811/150‑1 (MQ=60)
                                                                                                                               CCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAG                <  SRR19973083.1398233/150‑1 (MQ=60)
                                                                                                                                CTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGT               <  SRR19973083.1608702/150‑1 (MQ=60)
                                                                                                                                 TTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTA              >  SRR19973083.1665112/1‑150 (MQ=60)
                                                                                                                                 TTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTA              >  SRR19973083.2139199/1‑150 (MQ=60)
                                                                                                                                 TTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTA              >  SRR19973083.2139202/1‑150 (MQ=60)
                                                                                                                                 TTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTA              >  SRR19973083.1664801/1‑150 (MQ=60)
                                                                                                                                 TTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTA              >  SRR19973083.1664710/1‑150 (MQ=60)
                                                                                                                                         ATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAG      <  SRR19973083.5018451/150‑1 (MQ=60)
                                                                                                                                          TATAAATCGAGATTAGAACTGGCAAACCGGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGC     <  SRR19973083.1096527/150‑1 (MQ=60)
                                                                                                                                            TCATTCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAC   <  SRR19973083.1723906/150‑1 (MQ=60)
                                                                                                                                            TAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAC   <  SRR19973083.3174626/150‑1 (MQ=60)
                                                                                                                                             AAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACC  <  SRR19973083.4202622/150‑1 (MQ=60)
                                                                                                                                             |                                                                                                                                                     
GGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACC  >  CP009273/2304975‑2305265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑