Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,305,116 C→A 92.5% intergenic (‑103/+9) apbE ← / ← ompC putative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,305,1160CA92.5% 731.3 / 48.1 240intergenic (‑103/+9)apbE/ompCputative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base C (12/6);  new base A (113/109);  total (125/115)
Fisher's exact test for biased strand distribution p-value = 2.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01

Only 100 of 242 total aligned reads displayed.

GAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAG  >  CP009273/2304969‑2305261
                                                                                                                                                   |                                                                                                                                                 
gagCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGATTATTGCCATCCGTGATTATCCTCATGCGAACGGTCGCAAGAGAACACCAAAAAACTGAGTTTGTACGCTGAAAAAAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATaaa                                                                                                                                                 <  1:736412/150‑1 (MQ=255)
   cgcgGCAGCCAGCAGTGCCACGCGGGTACAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGATTTTGTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCg                                                                                                                                              <  2:3795990/150‑1 (MQ=255)
      ggCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCgaga                                                                                                                                           >  2:4007403/1‑150 (MQ=255)
      ggCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCgaga                                                                                                                                           >  2:1054243/1‑150 (MQ=255)
      ggCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCgaga                                                                                                                                           >  2:1672109/1‑150 (MQ=255)
       gCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTAAACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGAt                                                                                                                                          <  2:2325085/150‑1 (MQ=255)
         agccagcTGTGCCACGCGGGTAAAGCTTTTTTCCATACGTTATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGATTTTGTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTa                                                                                                                                        <  1:4256657/150‑1 (MQ=255)
         agccagcAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCACGAGTACACAAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTa                                                                                                                                        <  2:3890465/150‑1 (MQ=255)
         agccagcAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTa                                                                                                                                        <  2:3890970/150‑1 (MQ=255)
            cagcagTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGaa                                                                                                                                     >  2:1411606/1‑150 (MQ=255)
            cagcagTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGaa                                                                                                                                     >  1:2985405/1‑150 (MQ=255)
             agcagTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAAc                                                                                                                                    >  2:4291563/1‑150 (MQ=255)
              gcagTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGTGTTTGTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACt                                                                                                                                   <  1:3577128/150‑1 (MQ=255)
               cagTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTg                                                                                                                                  <  2:3498410/150‑1 (MQ=255)
               cagGGCCACGCGGGTAACGCTTATTTCCATCCGGGATTAACCTCATGCGACCGGTCGCACGAGTACCCCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAACTCGAGATTAGAACTg                                                                                                                                  <  2:1172641/150‑1 (MQ=255)
                 gTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTggc                                                                                                                                >  1:2372256/1‑149 (MQ=255)
                  tGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTggca                                                                                                                               >  1:1044448/1‑148 (MQ=255)
                    ccACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCaaa                                                                                                                             >  2:3119106/1‑150 (MQ=255)
                     cACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAAc                                                                                                                            >  2:1177736/1‑150 (MQ=255)
                        gcgGGTAAAGCTTATTTCCATAGGTGATTATCCTCATGCGAAGGGTCGCAAGAGTACACCAAAAACCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAg                                                                                                                         <  1:3883197/150‑1 (MQ=255)
                             tAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCa                                                                                                                    >  2:3308344/1‑150 (MQ=255)
                               aaGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCaga                                                                                                                  >  1:3758570/1‑150 (MQ=255)
                                aGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCagag                                                                                                                 >  2:3759318/1‑150 (MQ=255)
                                  cTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCt                                                                                                               >  1:4691806/1‑150 (MQ=255)
                                   ttATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTa                                                                                                              >  2:1302220/1‑150 (MQ=255)
                                    tATTTCCATACGTGATTATCCTCATGCGAACGGTCTCAAGAGTACACAAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCGGTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTAc                                                                                                             >  1:2283932/1‑150 (MQ=255)
                                    tATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTAGGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTAc                                                                                                             <  2:2425494/150‑1 (MQ=255)
                                       ttCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGAt                                                                                                          <  2:1601284/150‑1 (MQ=255)
                                       tacaaTACTTGATTATCCTCCTGCGACCGGTCGCAAGAGTACACCAAAAAACTGTTTTTGTACGCTGAAAACATTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGAt                                                                                                          <  2:872045/146‑1 (MQ=255)
                                          cATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGtt                                                                                                       <  2:1962215/150‑1 (MQ=255)
                                          cATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGtt                                                                                                       >  1:2962674/1‑150 (MQ=255)
                                           aTACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTa                                                                                                      >  1:3514743/1‑150 (MQ=255)
                                           aTACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTa                                                                                                      >  1:1807848/1‑150 (MQ=255)
                                           aTACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTa                                                                                                      >  1:4212032/1‑150 (MQ=255)
                                           aTACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTa                                                                                                      >  1:897685/1‑150 (MQ=255)
                                           aTACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTa                                                                                                      >  1:4351872/1‑150 (MQ=255)
                                              cGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCa                                                                                                   >  2:2965514/1‑150 (MQ=255)
                                               gTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAg                                                                                                  >  2:2266866/1‑150 (MQ=255)
                                                  aTTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAgtgt                                                                                               <  2:788945/150‑1 (MQ=255)
                                                      tCCTCATGCGACCGGTCGCAAGAGAACACCAAAAAACGGAGTTTGTCCGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAt                                                                                           <  1:2164529/150‑1 (MQ=255)
                                                      tCCTCATGCGAACGGTCGCAAGATTACACAAAAAAACTGAGTTTTTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAt                                                                                           <  1:1409590/150‑1 (MQ=255)
                                                      tCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAt                                                                                           <  2:370040/150‑1 (MQ=255)
                                                       ccTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGGTGATg                                                                                          >  1:3629320/1‑150 (MQ=255)
                                                        cTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGc                                                                                         >  1:55717/1‑150 (MQ=255)
                                                        cTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGc                                                                                         >  1:4102385/1‑150 (MQ=255)
                                                        cTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGc                                                                                         >  2:2885079/1‑150 (MQ=255)
                                                           aTGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAg                                                                                      >  1:4354137/1‑150 (MQ=255)
                                                           aTGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAg                                                                                      >  2:3437333/1‑150 (MQ=255)
                                                            tGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTGCGATAGAAATCGAGATTAGACCTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGc                                                                                     >  2:1875057/1‑150 (MQ=255)
                                                              cGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGt                                                                                   >  1:4589632/1‑150 (MQ=255)
                                                               gAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTc                                                                                  >  2:3565415/1‑150 (MQ=255)
                                                                aaCGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCa                                                                                 >  2:4743388/1‑150 (MQ=255)
                                                                  cGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTTAAAAAAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACg                                                                               <  1:1438565/150‑1 (MQ=255)
                                                                    gTCGCAAGAGTACCCCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAg                                                                             <  1:331950/150‑1 (MQ=255)
                                                                      cGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATACATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTg                                                                           <  2:4691806/150‑1 (MQ=255)
                                                                      cGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTg                                                                           <  1:2766615/150‑1 (MQ=255)
                                                                       gCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGa                                                                          <  2:4381326/150‑1 (MQ=255)
                                                                           gagTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTg                                                                      <  2:2297965/150‑1 (MQ=255)
                                                                            agTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTgg                                                                     >  1:1931445/1‑150 (MQ=255)
                                                                            agTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTgg                                                                     >  2:4498447/1‑150 (MQ=255)
                                                                            agTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTgg                                                                     >  2:2239059/1‑150 (MQ=255)
                                                                             gTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGt                                                                    <  2:1735879/150‑1 (MQ=255)
                                                                                 acCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtc                                                                >  1:3553866/1‑150 (MQ=255)
                                                                                 acCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtc                                                                >  1:1244221/1‑150 (MQ=255)
                                                                                   cAAAAAACAGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCATGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcga                                                              >  2:1641559/1‑149 (MQ=255)
                                                                                     aaaaaCTTAGTTTGTACGATGAAAAAAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTcc                                                            <  2:73729/150‑1 (MQ=255)
                                                                                     aaaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTcc                                                            <  2:2466538/150‑1 (MQ=255)
                                                                                     aaaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTcc                                                            >  2:2069680/1‑150 (MQ=255)
                                                                                      aaaaCTTAGTTTGAACGCTTAAAAAAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCca                                                           <  1:30424/150‑1 (MQ=255)
                                                                                       acaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcag                                                          <  1:3952531/148‑1 (MQ=255)
                                                                                         aCTGATTTTGTCCGCTCAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcagca                                                        <  1:1210739/150‑1 (MQ=255)
                                                                                         aCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcagca                                                        >  2:470647/1‑150 (MQ=255)
                                                                                          cTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcagcag                                                       >  1:2608140/1‑150 (MQ=255)
                                                                                           tGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAgg                                                      >  1:3313641/1‑150 (MQ=255)
                                                                                             aGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGtt                                                    <  2:3015963/150‑1 (MQ=255)
                                                                                                  gTACGCTCAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAttt                                               <  1:2041538/150‑1 (MQ=255)
                                                                                                     cGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGt                                            >  1:1282377/1‑150 (MQ=255)
                                                                                                        tGAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGt                                         <  1:4175318/150‑1 (MQ=255)
                                                                                                         gAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCCGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTc                                        >  1:926476/1‑150 (MQ=255)
                                                                                                           aaaCAATGAAAAATGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTGGTCGTCCAGCAGGTTGATTTTGTAGTCaa                                      >  2:1807640/1‑150 (MQ=255)
                                                                                                               aaTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGGTGATGTTGTAGTCAACGtc                                  >  2:593486/1‑149 (MQ=255)
                                                                                                                aTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAg                                 <  1:142045/150‑1 (MQ=255)
                                                                                                                 tGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg                                >  1:3366192/1‑150 (MQ=255)
                                                                                                                  gAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGt                               >  2:3812838/1‑150 (MQ=255)
                                                                                                                  gAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGt                               <  1:1116813/150‑1 (MQ=255)
                                                                                                                   aaaaaaGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTg                              >  2:1962370/1‑150 (MQ=255)
                                                                                                                   aaaaaaGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTg                              >  1:3863792/1‑150 (MQ=255)
                                                                                                                     aaaaGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGa                            >  1:4298493/1‑150 (MQ=255)
                                                                                                                       aaGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACa                          >  2:3920507/1‑150 (MQ=255)
                                                                                                                       aaGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACa                          >  2:1509153/1‑150 (MQ=255)
                                                                                                                           gCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGANATGtt                      <  1:187065/150‑1 (MQ=255)
                                                                                                                             ccGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt                    >  2:3039967/1‑150 (MQ=255)
                                                                                                                              cGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttttt                   <  1:4286410/150‑1 (MQ=255)
                                                                                                                                cAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt                 >  1:4476446/1‑150 (MQ=255)
                                                                                                                                        ttGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtag         <  1:2222110/150‑1 (MQ=255)
                                                                                                                                         tGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt        <  2:2770677/150‑1 (MQ=255)
                                                                                                                                            tCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg     <  1:2885079/150‑1 (MQ=255)
                                                                                                                                            tCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg     <  1:2200714/150‑1 (MQ=255)
                                                                                                                                             cGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggt    <  1:3119106/150‑1 (MQ=255)
                                                                                                                                              gATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggta   >  2:2502863/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAG  >  CP009273/2304969‑2305261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAC  >  CP009273/2304967‑2305264
                                                                                                                                                     |                                                                                                                                                    
atgcGCGCGGCCGCCAGCCGTGCCACGCGGGTAACGCTTATTCCCATCCTTTATTATCCTCATCCGAACGGCCGCAAGATAACCCCAAAAAACTGAGTTTGCACACTGAAAACAACCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATA                                                                                                                                                      <  SRR19973078.1846423/146‑1 (MQ=60)
AAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATA                                                                                                                                                      >  SRR19973078.3892401/1‑150 (MQ=60)
AAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATA                                                                                                                                                      >  SRR19973078.1204684/1‑150 (MQ=60)
  tcgggcgtcagcaagctgtgcaaggctgttaaatttttttaaaatactttattaacatcttgctaacggtccaatgactacaaaaaaaaactttttttctccgatcaaaaaaataAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAA                                                                                                                                                    <  SRR19973078.102591/35‑1 (MQ=60)
  GAGCGCGGCAGCCAGCAGTGCCACGCGGGTAACGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGAAAGTGTACACCAAAAAACTGTGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAA                                                                                                                                                    <  SRR19973078.740512/150‑1 (MQ=60)
  GAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGATTATTGCCATCCGTGATTATCCTCATGCGAACGGTCGCAAGAGAACACCAAAAAACTGAGTTTGTACGCTGAAAAAAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAA                                                                                                                                                    <  SRR19973078.740852/150‑1 (MQ=60)
       gggcagccagctgtgccagggggttaaagtttttttcaatatgttattaacctactgcgacgggtcccaagagaacacaaaaaaagtgttTTTGTACGCTTAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAG                                                                                                                                               <  SRR19973078.2498503/60‑1 (MQ=60)
       cggccgcccgctgtgcctgggggttaacgcttttttccttcgtttttttccctcatgctaacggtccaaagttaaaacaaaaaaaggttttttttccggttaaaaaattcAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAG                                                                                                                                               <  SRR19973078.525792/40‑1 (MQ=60)
          ccgccacctgtgcccgggggtaaaagttaattacaatagtttattaccctcttcctacgggtccaattattacacaaaaaacctgcttttgtccgctaaaaccaataAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATT                                                                                                                                            <  SRR19973078.4083051/43‑1 (MQ=60)
          CCGCCAGCTGTGCCACGGGGTTAAAGATTATTTCAATACTTTATTACCCACATGCTAACGGTCGAAAGAGTACACAAAAAAACTGTGTTTGTACGCTTAAAAAAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATT                                                                                                                                            <  SRR19973078.3915714/150‑1 (MQ=58)
          CAGCCAGCAGTGCCACGGGGGTAAAGATTATTTCCATAGGTGATTAGCCTCATGCGAACGGTCGAAAGAGTACACCAAAAAACGGAGTTTTTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATT                                                                                                                                            <  SRR19973078.3915721/150‑1 (MQ=60)
           AGCCAGCTGTGCCACGCGGGTAAAGCTTTTTTCCATACGTTATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGATTTTGTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTA                                                                                                                                           <  SRR19973078.4280861/150‑1 (MQ=60)
            GCCAGCTGTGCCACGCGGGTAAAGTTTTTTTCCATACGTTATTATCCTCATGCGAACGGTCGCAAGAGTACACAAAAAAACTTTGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAG                                                                                                                                          <  SRR19973078.1831553/150‑1 (MQ=60)
            GCCAGCAGTGCCACGCGGGTAAAGATTATTTCCATCCGTGATTATCCTCATGCGAACGGTCGCATGAGTACACCAAAACACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAG                                                                                                                                          <  SRR19973078.1569307/150‑1 (MQ=60)
             CCCGCAGGGCCACGGGGGTAAAGCTTATTTCCATTCGTTATTATCCTAATGCTAACGTTCGAAAGATTACACAAAAAAATGGTTTTTTTACGCTTAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGA                                                                                                                                         <  SRR19973078.2940014/150‑1 (MQ=48)
              CAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAA                                                                                                                                        >  SRR19973078.3002439/1‑150 (MQ=60)
               AGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAAC                                                                                                                                       >  SRR19973078.548924/1‑150 (MQ=60)
                GCAGTGCCACGGGGGTAAAGCTTATTTCCATACGTGTTTATCCTCATGCGAACGGTCGCAAGTGTACACCAAAAAACTGTGTTTGTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACT                                                                                                                                      <  SRR19973078.3597241/150‑1 (MQ=60)
                GCAGTGCCACGCGGTTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACAAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACT                                                                                                                                      <  SRR19973078.3596957/150‑1 (MQ=60)
                GCAGTGCCACGCGGTTAAAGCTTATTTCAATACGTTATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGATTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACT                                                                                                                                      <  SRR19973078.3595404/150‑1 (MQ=60)
                GCAGTGCCACGCGGTTAAAGATTTTTTCCATACGTTATTATCCTCATGCGAACGGTCGAAAGAGTACACAAAAAAACTTAGTTTGTACGCTGAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACT                                                                                                                                      <  SRR19973078.3484778/150‑1 (MQ=60)
                   GTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGC                                                                                                                                   >  SRR19973078.2385666/1‑150 (MQ=60)
                    TGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCA                                                                                                                                  >  SRR19973078.1050907/1‑150 (MQ=60)
                        acggggttaattcttttttccttaggtgtttatcctcttgctaagggtggcaagattacaacaaaaaagttatTTTGTACGCTGAAAAAAATGAAAAAAGGGCCCGCAGGCCCTTTGTTAGATATAAATCGAGATTAGAACTGGCTAACC                                                                                                                              <  SRR19973078.3950572/77‑1 (MQ=60)
                        ACGCGGGTAAAGATTATTTCCATACTTTATTATCCTCATGCTACCGGTCGCAATAGTACACCAAAAACCTGAGTTTGTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACC                                                                                                                              <  SRR19973078.2181551/150‑1 (MQ=60)
                          GCGGGTAAAGCTTATTTCCATAGGTGATTATCCTCATGCGAAGGGTCGCAAGAGTACACCAAAAACCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAG                                                                                                                            <  SRR19973078.3905334/150‑1 (MQ=60)
                            GGGTAAAGATTATTTCCATACGTTATTATCCTCATGCGAACGGTCGAAAGAGTACACCAAAAAACTGATTTTGTACGATGAAAAAATTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGAC                                                                                                                          <  SRR19973078.1903880/150‑1 (MQ=60)
                             GGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGTCAAACCAGACC                                                                                                                         >  SRR19973078.920219/1‑150 (MQ=60)
                              taaaagttttttaccatctgttattaacaactcgctacgggacgaaatataaaacaaaaaaactttgttttTACGCTTAAAAAAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCC                                                                                                                        <  SRR19973078.2053104/79‑1 (MQ=60)
                                 AAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGA                                                                                                                     >  SRR19973078.3780153/1‑150 (MQ=60)
                                  AGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAG                                                                                                                    >  SRR19973078.4310547/1‑150 (MQ=60)
                                    CTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCT                                                                                                                  >  SRR19973078.4718289/1‑150 (MQ=60)
                                     ttattacaatcgtttATTATCCTAATGCGACGGGTCGAAAGAGTACACCAAAAAAATGTTTTTTTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTA                                                                                                                 <  SRR19973078.3649936/135‑1 (MQ=60)
                                      tttttcaatacttttttatcctcatgctaacggtcgatatattacaaaaaaaaactttttttttacgattAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTAC                                                                                                                <  SRR19973078.4585799/80‑1 (MQ=60)
                                      TATTTCCATACGTGATTATCCTCATGCGAACGGTCTCAAGAGTACACAAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCGGTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTAC                                                                                                                >  SRR19973078.2297079/1‑150 (MQ=60)
                                      TATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTAC                                                                                                                >  SRR19973078.997727/1‑150 (MQ=60)
                                      TATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTAC                                                                                                                >  SRR19973078.381240/1‑150 (MQ=60)
                                      TATTTCAATTCGTTATTATCATAATGCGAACGGTCGAAAGATAACACAAAAAAACTTATTTTTTACGTTTAAAAAAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTAC                                                                                                                <  SRR19973078.2141868/150‑1 (MQ=60)
                                      TATTACAATACGTGATTATCCTCATGCGAACGGTCGAAAGATTAACCCAAAAAACTGTGTTTTTCCGCTGAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTAC                                                                                                                <  SRR19973078.2141939/150‑1 (MQ=60)
                                       ATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACG                                                                                                               >  SRR19973078.1137031/1‑150 (MQ=60)
                                           CCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGT                                                                                                           >  SRR19973078.2391481/1‑150 (MQ=60)
                                           CCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGT                                                                                                           >  SRR19973078.2391465/1‑150 (MQ=60)
                                            CATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTT                                                                                                          >  SRR19973078.2979582/1‑150 (MQ=60)
                                             ATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTA                                                                                                         >  SRR19973078.4376350/1‑150 (MQ=60)
                                             ATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTA                                                                                                         >  SRR19973078.4376307/1‑150 (MQ=60)
                                             ATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTA                                                                                                         >  SRR19973078.3534678/1‑150 (MQ=60)
                                             ATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTA                                                                                                         >  SRR19973078.4236027/1‑150 (MQ=60)
                                             ATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTA                                                                                                         >  SRR19973078.1818773/1‑150 (MQ=60)
                                             ATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTA                                                                                                         >  SRR19973078.903254/1‑150 (MQ=60)
                                                     taacccacagcctaacgcgcgcaacctaacacaaaaaaccggattttgtactcttaaaaaaattAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTT                                                                                                 <  SRR19973078.512671/86‑1 (MQ=60)
                                                       ATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGA                                                                                               >  SRR19973078.3550410/1‑150 (MQ=60)
                                                        tcctcatgcgcacggaccaaagataacaaaaaaaaactgagtttgtcccaacaaaccaataaaaaccGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAT                                                                                              <  SRR19973078.3615740/83‑1 (MQ=60)
                                                        TCCTCATGCTAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAT                                                                                              <  SRR19973078.1419300/150‑1 (MQ=60)
                                                        TCCTCATGCGACCGGTCGCAAGAGAACACCAAAAAACGGAGTTTGTCCGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAT                                                                                              <  SRR19973078.2177205/150‑1 (MQ=60)
                                                        TCCTCATGCGAACGGTCGCAAGATTACACAAAAAAACTGAGTTTTTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAT                                                                                              <  SRR19973078.1418318/150‑1 (MQ=60)
                                                         CCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGGTGATG                                                                                             >  SRR19973078.3649898/1‑150 (MQ=60)
                                                          CTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGC                                                                                            >  SRR19973078.56311/1‑150 (MQ=60)
                                                          CTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGC                                                                                            >  SRR19973078.4127430/1‑150 (MQ=60)
                                                          CTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGC                                                                                            >  SRR19973078.4125816/1‑150 (MQ=60)
                                                             ATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAG                                                                                         >  SRR19973078.4378630/1‑150 (MQ=60)
                                                             ATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAG                                                                                         >  SRR19973078.4378291/1‑150 (MQ=60)
                                                                CGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGT                                                                                      >  SRR19973078.4615633/1‑150 (MQ=60)
                                                                CGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGT                                                                                      >  SRR19973078.4615801/1‑150 (MQ=60)
                                                                  AACGGTCGCAAGCGTACACCAAAACCGGGCGTTTGTCCGCTTAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCA                                                                                    <  SRR19973078.429915/150‑1 (MQ=60)
                                                                    CGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTTAAAAAAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACG                                                                                  <  SRR19973078.1447430/150‑1 (MQ=60)
                                                                      GTCGCAAGAGTACCCCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAG                                                                                <  SRR19973078.334115/150‑1 (MQ=60)
                                                                      GTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATGAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAG                                                                                <  SRR19973078.334038/150‑1 (MQ=60)
                                                                      GTCGAAAGAGTACACAAAAAAACTGCGTTTGTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAG                                                                                <  SRR19973078.222781/150‑1 (MQ=60)
                                                                        CGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTG                                                                              <  SRR19973078.2782073/150‑1 (MQ=60)
                                                                            ATAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACT                                                                          <  SRR19973078.4212705/150‑1 (MQ=60)
                                                                             GAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTG                                                                         >  SRR19973078.486159/1‑150 (MQ=60)
                                                                              AGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGG                                                                        >  SRR19973078.1942791/1‑150 (MQ=60)
                                                                              AGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGG                                                                        >  SRR19973078.1942042/1‑150 (MQ=60)
                                                                              AGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTCCGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGG                                                                        >  SRR19973078.3299815/1‑150 (MQ=60)
                                                                              AGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGG                                                                        >  SRR19973078.2812829/1‑150 (MQ=60)
                                                                              AGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGG                                                                        >  SRR19973078.2913434/1‑150 (MQ=60)
                                                                                   ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTC                                                                   >  SRR19973078.1251899/1‑150 (MQ=60)
                                                                                   ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTC                                                                   >  SRR19973078.1251173/1‑150 (MQ=60)
                                                                                   ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTC                                                                   >  SRR19973078.3573913/1‑150 (MQ=60)
                                                                                      AAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTC                                                                <  SRR19973078.2279960/150‑1 (MQ=60)
                                                                                        AAAACTTAGTTTGAACGCTTAAAAAAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCA                                                              <  SRR19973078.30782/150‑1 (MQ=60)
                                                                                        AAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTGCGAAATAAATCGAGATTAGAAATGGCAAACCAGACCCAGAGCTCCGATGTTATCAGTGTTGAGGCCCGCGTCACGAGTGACCTGGTGGTCGTCCA                                                              >  SRR19973078.859544/1‑150 (MQ=60)
                                                                                         ACACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAG                                                             <  SRR19973078.3974904/150‑1 (MQ=60)
                                                                                           actttttttgtccgaTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCA                                                           <  SRR19973078.1218184/135‑1 (MQ=60)
                                                                                           ACTGATTTTGTCCGCTCAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCA                                                           <  SRR19973078.1218076/150‑1 (MQ=60)
                                                                                           ACTGAGTTTGTCCGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCA                                                           <  SRR19973078.4170863/150‑1 (MQ=60)
                                                                                           ACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCA                                                           >  SRR19973078.352344/1‑150 (MQ=60)
                                                                                            CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAG                                                          >  SRR19973078.1442547/1‑150 (MQ=60)
                                                                                            CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAG                                                          >  SRR19973078.2623051/1‑150 (MQ=60)
                                                                                             TGAGTTTGTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGG                                                         <  SRR19973078.2262159/150‑1 (MQ=60)
                                                                                             TGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGG                                                         >  SRR19973078.3332562/1‑150 (MQ=60)
                                                                                               AGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTT                                                       >  SRR19973078.3997402/1‑150 (MQ=60)
                                                                                                    GTACGCTCAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTT                                                  <  SRR19973078.2053511/150‑1 (MQ=60)
                                                                                                      ACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTG                                                <  SRR19973078.754009/150‑1 (MQ=60)
                                                                                                       CGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGT                                               >  SRR19973078.1290374/1‑150 (MQ=60)
                                                                                                       CGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGT                                               >  SRR19973078.3088321/1‑150 (MQ=60)
                                                                                                       CGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGGTGATTTTGT                                               >  SRR19973078.1290053/1‑150 (MQ=60)
                                                                                                        GCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTA                                              >  SRR19973078.4463455/1‑150 (MQ=60)
                                                                                                         CTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGGTGATTTTGTAG                                             >  SRR19973078.4346461/1‑150 (MQ=60)
                                                                                                          TGAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGT                                            <  SRR19973078.4199149/150‑1 (MQ=60)
                                                                                                           GAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCCGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTC                                           >  SRR19973078.932263/1‑150 (MQ=60)
                                                                                                           GAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTC                                           >  SRR19973078.3340130/1‑150 (MQ=60)
                                                                                                              AACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAAC                                        <  SRR19973078.1956244/150‑1 (MQ=60)
                                                                                                              AACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAAC                                        >  SRR19973078.2997865/1‑150 (MQ=60)
                                                                                                                CAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGT                                      >  SRR19973078.1090133/1‑150 (MQ=60)
                                                                                                                CAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGT                                      >  SRR19973078.1371821/1‑150 (MQ=60)
                                                                                                                  ATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAG                                    <  SRR19973078.143354/150‑1 (MQ=60)
                                                                                                                  ATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAG                                    >  SRR19973078.2384337/1‑150 (MQ=60)
                                                                                                                   TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGG                                   >  SRR19973078.3385455/1‑150 (MQ=60)
                                                                                                                    GAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGT                                  <  SRR19973078.1123575/150‑1 (MQ=60)
                                                                                                                     AAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTG                                 >  SRR19973078.3885874/1‑150 (MQ=60)
                                                                                                                     AAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTG                                 >  SRR19973078.3885873/1‑150 (MQ=60)
                                                                                                                       AAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGA                               >  SRR19973078.4322821/1‑150 (MQ=60)
                                                                                                                        AAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGAC                              >  SRR19973078.4431034/1‑150 (MQ=60)
                                                                                                                         AAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTGCGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGTTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACA                             >  SRR19973078.427001/1‑150 (MQ=60)
                                                                                                                             GCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGANATGTT                         <  SRR19973078.188621/150‑1 (MQ=60)
                                                                                                                                CGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTT                      <  SRR19973078.1310360/150‑1 (MQ=60)
                                                                                                                                CGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTT                      <  SRR19973078.4311755/150‑1 (MQ=60)
                                                                                                                                CGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTT                      <  SRR19973078.4310701/150‑1 (MQ=60)
                                                                                                                                 GCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTG                     <  SRR19973078.375543/150‑1 (MQ=60)
                                                                                                                                  CAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGT                    >  SRR19973078.4501781/1‑150 (MQ=60)
                                                                                                                                   AGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTT                   <  SRR19973078.1793402/150‑1 (MQ=60)
                                                                                                                                   AGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTT                   <  SRR19973078.3148871/150‑1 (MQ=60)
                                                                                                                                         TTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTA             >  SRR19973078.2731693/1‑150 (MQ=60)
                                                                                                                                          TTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAG            <  SRR19973078.2235059/150‑1 (MQ=60)
                                                                                                                                           TGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGTAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGT           <  SRR19973078.3023893/150‑1 (MQ=60)
                                                                                                                                              TCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGG        <  SRR19973078.2901465/150‑1 (MQ=60)
                                                                                                                                              TCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGG        <  SRR19973078.2213580/150‑1 (MQ=60)
                                                                                                                                               CGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGT       <  SRR19973078.3136625/150‑1 (MQ=60)
                                                                                                                                               CGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGT       >  SRR19973078.3774695/1‑150 (MQ=60)
                                                                                                                                                 ATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAG     <  SRR19973078.3021810/150‑1 (MQ=60)
                                                                                                                                                   ATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCA   <  SRR19973078.1937982/150‑1 (MQ=60)
                                                                                                                                                   ATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAGTTTGTAGTCAACGTCGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCA   >  SRR19973078.1661325/1‑150 (MQ=60)
                                                                                                                                                    TAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAC  >  SRR19973078.1041505/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
AAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAC  >  CP009273/2304967‑2305264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑