Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,305,116 C→A 93.6% intergenic (‑103/+9) apbE ← / ← ompC putative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,305,1160CA93.6% 719.3 / 33.6 221intergenic (‑103/+9)apbE/ompCputative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base C (8/7);  new base A (110/96);  total (118/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 224 total aligned reads displayed.

AGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACC  >  CP009273/2304968‑2305265
                                                                                                                                                     |                                                                                                                                                     
agagCGCGGCAGCCAGCAGTGCCACGCGGTTAAAGCTTTTTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCa                                                                                                                                                      <  2:3654061/150‑1 (MQ=255)
 gcgcgcgcCAGCCAGCAGTGCCACGCGGTTAAAGCTAATTTCCATACGTCATTATCCTCATGCGACCGGTCGAAAGAGTACACCAAAAAAATGAGTTGGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATaaa                                                                                                                                                     <  2:2525560/148‑1 (MQ=255)
  agCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAAt                                                                                                                                                    >  1:1526519/1‑150 (MQ=255)
   gcgcGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACTTTATTATCCTCATGCGAACGGTCGCAATAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATc                                                                                                                                                   <  1:3106382/150‑1 (MQ=255)
   gcgcGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTTTTTATCCTCTTGCGAACGGTCGCAAGTGTACACAAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATc                                                                                                                                                   <  1:4252386/150‑1 (MQ=255)
   gcgcGGCAGCAAGCAGTGCCACGCGGGTAAAGATTATTTCCATACGTTATTATCCTCATGCGAACGTTCGCAAGAGTACACCAAAAAACTGAGTTTTTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATc                                                                                                                                                   <  1:1134488/150‑1 (MQ=255)
    cgcgGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCg                                                                                                                                                  <  2:558238/150‑1 (MQ=255)
    cgcgGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCg                                                                                                                                                  >  2:2976209/1‑150 (MQ=255)
       ggCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCgaga                                                                                                                                               >  2:4147825/1‑150 (MQ=255)
         cagccagcAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGAtt                                                                                                                                             <  2:2264045/150‑1 (MQ=255)
          agccagcTGTGCCACGCGTTTAACGATTATTACCATACTTTATTACCCTCATGCTAACGGTCGCAACAGTACACAAAAAAACTGATTTTTTACGCTCAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTa                                                                                                                                            <  1:3750306/150‑1 (MQ=255)
           gccagcTGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCTTGCGACCGGTCGCACGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAg                                                                                                                                           <  1:1505461/150‑1 (MQ=255)
           gccagcAGTGCCCCGGGGGTAAAGCTTTTTACCATAGGTGATTATCATCTTGCGCACGGTCGCAAGAGTACACCAAAAAACTGAGTTTTTACGCTGAAAACAA‑TTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAg                                                                                                                                           <  1:625939/150‑1 (MQ=255)
              agcagTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGCGATTAGAAc                                                                                                                                        >  2:2105471/1‑150 (MQ=255)
              agcagTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAAc                                                                                                                                        >  2:2376632/1‑150 (MQ=255)
                 agTGCCACGCGGTTAAAGCTTTTTTCAATACGTTATTATCCTCATGCTAACGGTCGCAAGAGTACACCAAAACACTGATTTTTTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTgg                                                                                                                                     <  2:2408220/150‑1 (MQ=255)
                   tGCCACGGGGTTAAATATTATTTCAATTCTTTATAAACCTCATCCGAACGTTCGAAAGATAACAAAAAAAAACTGTGTTTTTACGATCAAAAAAA‑TTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTggca                                                                                                                                   <  1:1992136/150‑3 (MQ=255)
                      cACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAAc                                                                                                                                >  2:367315/1‑150 (MQ=255)
                      cACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAAc                                                                                                                                >  2:4201148/1‑150 (MQ=255)
                          cgGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGa                                                                                                                            >  1:4044263/1‑150 (MQ=255)
                            ggTAAAGCTTATTTCCATACGTGATTAACCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGAcc                                                                                                                          >  1:4156549/1‑150 (MQ=255)
                              tAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCa                                                                                                                        >  1:2874236/1‑150 (MQ=255)
                                   cTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCt                                                                                                                   >  1:2146897/1‑150 (MQ=255)
                                   cTTATTTCCATACGTGATTATCCACATGCCCACGGTCGCAAGAGTACACCAAAAAACTGATTTTGTACGCTCAAAAAAA‑TCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAAACGAGATTAGAACTGGTAAACCAGACCCAGAGCt                                                                                                                   <  2:3190319/150‑1 (MQ=255)
                                     tATTTCCATAAGTGATTATCCTCATTCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGATGAAAACAA‑TGAAAAAAGGGCCCGCAGGACCTTTTTTCGATATAAATAGAGATTAGAACTCTCAAACCAGACCCAGAGCTaa                                                                                                                 >  1:3349063/1‑149 (MQ=255)
                                      aTTTCCATACGTGATTATCCTCATGCCAACGGTCTAAATATAACACAAAAAAACTTTGTTTTTACGCTCAAAAAAT‑TCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACg                                                                                                                <  1:1648641/150‑1 (MQ=255)
                                       tttCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGa                                                                                                               >  1:4451457/1‑150 (MQ=255)
                                         tCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATg                                                                                                             >  1:2387427/1‑150 (MQ=255)
                                          ccATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGt                                                                                                            >  1:1161434/1‑150 (MQ=255)
                                          ccATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGt                                                                                                            >  1:4175580/1‑150 (MQ=255)
                                            aTACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTa                                                                                                          <  2:730337/150‑1 (MQ=255)
                                              aCGTTATTATCCTCATGCGACCGGTCGCACGAGTACACCAAAAACCTGCGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATc                                                                                                        <  1:606784/150‑1 (MQ=255)
                                                   aTTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTCTCAgtgt                                                                                                   >  2:1684951/1‑150 (MQ=255)
                                                      aTCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGa                                                                                                >  1:881536/1‑150 (MQ=255)
                                                       tCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTCCGATGTTATCAGTGTTGAt                                                                                               >  1:1266038/1‑150 (MQ=255)
                                                       tCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAt                                                                                               >  1:3660116/1‑150 (MQ=255)
                                                         cTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGc                                                                                             >  2:616090/1‑150 (MQ=255)
                                                          tCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGATTTTGTCCGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGcc                                                                                            <  2:1190245/150‑1 (MQ=255)
                                                          tCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGcc                                                                                            <  2:4211789/150‑1 (MQ=255)
                                                           cATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCa                                                                                           >  1:3458566/1‑150 (MQ=255)
                                                            aTGCGAACGGTCGCAAGAGTACCCCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAg                                                                                          <  2:1512266/150‑1 (MQ=255)
                                                            aTGCGAACGGTCGAAAGAGTACACAAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAg                                                                                          <  1:4657193/150‑1 (MQ=255)
                                                                gAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTc                                                                                      >  1:1126936/1‑150 (MQ=255)
                                                                 aaCGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCa                                                                                     <  2:1954181/150‑1 (MQ=255)
                                                                 aaCGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCa                                                                                     >  1:3250077/1‑150 (MQ=255)
                                                                   cGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACg                                                                                   >  1:1066893/1‑150 (MQ=255)
                                                                      tCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGt                                                                                <  2:3347256/150‑1 (MQ=255)
                                                                       cGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTg                                                                               >  2:3995835/1‑150 (MQ=255)
                                                                        gCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGa                                                                              >  1:529311/1‑150 (MQ=255)
                                                                        gCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGa                                                                              >  1:232934/1‑150 (MQ=255)
                                                                         cAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGaa                                                                             >  2:731354/1‑150 (MQ=255)
                                                                         cAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGaa                                                                             <  2:4578137/150‑1 (MQ=255)
                                                                         aaaGAGTACACAAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGaa                                                                             <  1:2673087/149‑1 (MQ=255)
                                                                            gagTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTg                                                                          >  1:3059170/1‑150 (MQ=255)
                                                                              taccaaaaaaaaaGTGTGTTTTTACGC‑GTAAACAATTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGt                                                                        <  1:2794704/143‑1 (MQ=255)
                                                                               tACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGtt                                                                       <  2:1551598/150‑1 (MQ=255)
                                                                               tACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGtt                                                                       <  2:4386230/150‑1 (MQ=255)
                                                                                acacCAAACAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTg                                                                      <  2:1063517/150‑1 (MQ=255)
                                                                                acacCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTg                                                                      >  1:1564027/1‑150 (MQ=255)
                                                                                acacCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTg                                                                      <  2:76181/150‑1 (MQ=255)
                                                                                acacCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTg                                                                      <  2:2842533/150‑1 (MQ=255)
                                                                                acacCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTg                                                                      <  2:497978/150‑1 (MQ=255)
                                                                                   ccAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcg                                                                   <  2:1125532/150‑1 (MQ=255)
                                                                                    cAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcgt                                                                  >  2:2689962/1‑150 (MQ=255)
                                                                                     aaaaaaCTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcgtc                                                                 >  1:516298/1‑150 (MQ=255)
                                                                                           cTGAGTTTGTACGCTGAAAACAA‑TGGAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcagcag                                                           >  2:552382/1‑150 (MQ=255)
                                                                                           cTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcagcag                                                           <  2:1775127/150‑1 (MQ=255)
                                                                                           cTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcagcag                                                           >  1:1695950/1‑150 (MQ=255)
                                                                                               gTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGAGGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGtgg                                                       >  2:3857368/1‑148 (MQ=255)
                                                                                                   gTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGAGGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAttt                                                   >  2:89333/1‑150 (MQ=255)
                                                                                                    tACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAtttt                                                  <  1:3168898/150‑1 (MQ=255)
                                                                                                      cGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGt                                                >  2:3128496/1‑150 (MQ=255)
                                                                                                        cTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAg                                              <  1:3107042/150‑1 (MQ=255)
                                                                                                        cTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAg                                              >  2:4667528/1‑150 (MQ=255)
                                                                                                        cTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAg                                              >  2:3746062/1‑150 (MQ=255)
                                                                                                         ttaaaaCAA‑TCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGt                                             <  1:143427/148‑1 (MQ=255)
                                                                                                         tGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGGTGATTTTGTAGt                                             >  1:4526149/1‑150 (MQ=255)
                                                                                                          gAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTc                                            <  2:211371/150‑1 (MQ=255)
                                                                                                          gAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCATTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTc                                            <  2:224695/150‑1 (MQ=255)
                                                                                                             aaCAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATGTTGTAGTCAAc                                         >  2:1360197/1‑150 (MQ=255)
                                                                                                               cAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt                                       >  1:2269979/1‑150 (MQ=255)
                                                                                                                     aaaaaaGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTg                                  <  1:336832/150‑1 (MQ=255)
                                                                                                                      aaaaaGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATGAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTgg                                 <  1:2381018/150‑1 (MQ=255)
                                                                                                                       aaaaGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGa                                >  2:4314724/1‑150 (MQ=255)
                                                                                                                         aaGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACa                              >  1:2641537/1‑150 (MQ=255)
                                                                                                                          aGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt                             <  1:4250950/150‑1 (MQ=255)
                                                                                                                           gggCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATg                            <  2:142816/150‑1 (MQ=255)
                                                                                                                               ccGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt                        <  2:1012307/150‑1 (MQ=255)
                                                                                                                                  cAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt                     >  2:1177690/1‑150 (MQ=255)
                                                                                                                                  cAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt                     >  2:4230438/1‑150 (MQ=255)
                                                                                                                                   aGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgtt                    >  1:2842975/1‑150 (MQ=255)
                                                                                                                                      cccTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGaa                 <  1:4203330/150‑1 (MQ=255)
                                                                                                                                        cTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagt               <  2:2146897/150‑1 (MQ=255)
                                                                                                                                         tttGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagta              <  2:3099210/150‑1 (MQ=255)
                                                                                                                                          ttGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtag             >  2:277709/1‑150 (MQ=255)
                                                                                                                                            gTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAgtagta           >  2:174717/1‑150 (MQ=255)
                                                                                                                                             ttCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGtagtag          >  2:3592027/1‑150 (MQ=255)
                                                                                                                                                gATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtc       >  2:688002/1‑149 (MQ=255)
                                                                                                                                                gATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggta       >  2:66106/1‑150 (MQ=255)
                                                                                                                                                  tataAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGc     >  1:3628658/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAA‑TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACC  >  CP009273/2304968‑2305265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAC  >  CP009273/2304969‑2305264
                                                                                                                                                   |                                                                                                                                                    
gggcgggccccccacctgtccccgggggtaacatcttttttcaaacggtgatcatccacctgcgaccggtcgaaatctaacacaaaaacacggattttttacgcctaaaaaaatcAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAA                                                                                                                                                    <  SRR19973082.4026088/35‑1 (MQ=60)
gcgggggccagcctgctgtgcctggggtttaatgcttttttccatcgttttttaccatattgcgacggttccaaatataaacaaaaaaatttgtttttttactctaaaaaaaatcAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAA                                                                                                                                                    <  SRR19973082.1624012/35‑1 (MQ=60)
GCGCGCGGCAGCCAGCAGGGCCCCGGGGTTAAAGCTTATTTCCATCCTTGTTTACCCTCTTGCGAACGGTCCAAATAGACCACAAAAAAACTTAGTTTGTACGCTCAAAAAAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAA                                                                                                                                                    <  SRR19973082.539285/150‑1 (MQ=38)
 AGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACGCCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAAT                                                                                                                                                   >  SRR19973082.3201505/1‑150 (MQ=60)
 AGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAAT                                                                                                                                                   >  SRR19973082.1536624/1‑150 (MQ=60)
  GCGCGGCAGCCAGCTGTGCCACGCGGGTAAAGCTTATTTCAATACGTGATTATCCTCAGGCGAACGGTCGCACGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATC                                                                                                                                                  <  SRR19973082.4277567/150‑1 (MQ=60)
  GCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTTTTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGTTTACACCAAAAAACTGTGTTTGTACGCTGAAAACATTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATC                                                                                                                                                  <  SRR19973082.1142029/150‑1 (MQ=60)
  GCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACTTTATTATCCTCATGCGAACGGTCGCAATAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATC                                                                                                                                                  <  SRR19973082.3125294/150‑1 (MQ=60)
  GCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTTTTTATCCTCTTGCGAACGGTCGCAAGTGTACACAAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATC                                                                                                                                                  <  SRR19973082.4278241/150‑1 (MQ=60)
  GCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTTATTATCCTCATGCGAACGGTCGCAAGAGTACACAAAAAAACTGAGTTTGTACGCTGAAAAAAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATC                                                                                                                                                  <  SRR19973082.3125300/150‑1 (MQ=60)
  GCGCGGCAGCAAGCAGTGCCACGCGGGTAAAGATTATTTCCATACGTTATTATCCTCATGCGAACGTTCGCAAGAGTACACCAAAAAACTGAGTTTTTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATC                                                                                                                                                  <  SRR19973082.1142038/150‑1 (MQ=60)
     CGGCAGCCAGCAGTGCCACGCGTTAAATGATTATTTCAATACGTTATTATCCTCATGCGAACGGTCGCACTAGTACACAAAAAACATGTTTTTGTACCCGTAAAACAATAAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAG                                                                                                                                               <  SRR19973082.265671/150‑1 (MQ=45)
      ggaagccttctgggcccggggttaaattcttattcaattcgtttattatcctcttgctaaggttcgaaatataaaacaaaaacactttGTTTGTACGATCAAAAAAATAAAAAACGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGA                                                                                                                                              <  SRR19973082.3216728/62‑1 (MQ=28)
      gcagtccttatttcgaggtcggttattgtttattacaacatttctttatactattcccaacggttgtatttgtacaaaaaaaacgggattttgtaggtttaaaactaaGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATATATCGAGA                                                                                                                                              <  SRR19973082.3216382/42‑1 (MQ=60)
      GGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGA                                                                                                                                              >  SRR19973082.1116289/1‑150 (MQ=60)
      GGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGA                                                                                                                                              >  SRR19973082.3520013/1‑150 (MQ=60)
       GCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATCCGTGATTATCCACATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGAT                                                                                                                                             <  SRR19973082.1151549/150‑1 (MQ=60)
         AGCCAGCTGTGCCACGCGTTTAACGATTATTACCATACTTTATTACCCTCATGCTAACGGTCGCAACAGTACACAAAAAAACTGATTTTTTACGCTCAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTA                                                                                                                                           <  SRR19973082.3773492/150‑1 (MQ=60)
          GCCAGCTGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCTTGCGACCGGTCGCACGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAG                                                                                                                                          <  SRR19973082.1515494/150‑1 (MQ=60)
          GCCAGCAGTGCCCCGGGGGTAAAGCTTTTTACCATAGGTGATTATCATCTTGCGCACGGTCGCAAGAGTACACCAAAAAACTGAGTTTTTACGCTGAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAG                                                                                                                                          <  SRR19973082.630325/150‑1 (MQ=60)
          GCCAGCAGTGCCACGGGGTTAAAGATTTTTTCAATACTTGATTATCCTCATGCTATCGGTCGCAAGAGTACACAAAAAAACTGTGTTTGTCCGCTGAAAAAAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAG                                                                                                                                          <  SRR19973082.629997/150‑1 (MQ=60)
          GCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGACCGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAG                                                                                                                                          <  SRR19973082.1515550/150‑1 (MQ=60)
                  tgccacggggttaaatattatttcaattctttataaacctcatccgaacgttcgaaagataacaaaAAAAAACTGTGTTTTTACGATCAAAAAAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCA                                                                                                                                  <  SRR19973082.2004651/84‑1 (MQ=53)
                   gccagggtgttaattatttttaccttcgtttattaacctcttgctaagggtctaaagataacacaaaaaaacttttttttTACGCTTAAAAAAATAAAAAACGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAA                                                                                                                                 <  SRR19973082.3222509/70‑1 (MQ=49)
                   GCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAA                                                                                                                                 >  SRR19973082.2082328/1‑150 (MQ=60)
                        gggtgtaatgtttttttccatagttgattatcatcttgctaacggtcgaatgatTACACAAAAAAACTGAGTTTGTACGCTTAAAAAATTTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAG                                                                                                                            <  SRR19973082.1265729/96‑1 (MQ=60)
                        ggggtTAAAGCTTTTTACCATACGTTATTATCCCCATGCTAAGGGTCCCAATAGAACCCCAAAAAATTGTGTTTGTACGCTAAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAG                                                                                                                            <  SRR19973082.2871995/145‑1 (MQ=60)
                         CGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGA                                                                                                                           >  SRR19973082.1360636/1‑150 (MQ=60)
                         CGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGA                                                                                                                           >  SRR19973082.1360512/1‑150 (MQ=60)
                         CGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGA                                                                                                                           >  SRR19973082.4069053/1‑150 (MQ=60)
                           GGTAAAGCTTATTTCCATACGTGATTAACCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACC                                                                                                                         >  SRR19973082.4181995/1‑150 (MQ=60)
                            GTAACTCTTATTTCCATACGTGATTAACCTATGGCGAACGGTCGAAATAGTACACAAAAAAACTGAGTTTGTCCGCTTAAAAAAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCC                                                                                                                        <  SRR19973082.3712940/150‑1 (MQ=60)
                             TAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCA                                                                                                                       >  SRR19973082.2891966/1‑150 (MQ=60)
                              AAAGCTTATTTCCATACGTTTTTATCCTCATGCGAACGGTCGCAAGAGTACACAAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAG                                                                                                                      <  SRR19973082.2697665/150‑1 (MQ=60)
                               AAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGA                                                                                                                     >  SRR19973082.648507/1‑150 (MQ=60)
                                  CTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTGAGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCT                                                                                                                  >  SRR19973082.2160545/1‑150 (MQ=60)
                                   ttatttcattcgttttttaccctcatgccaccggtccaatgacaacaaaaaaaaattgtttttgtccgctcaaaaaaataAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTA                                                                                                                 <  SRR19973082.816927/70‑1 (MQ=60)
                                   TTATTTCCATAGGTTATTATCCTCATGCTACCGGTCGCAAGACAACACAAAAAACCTGATTTTTTCCGCTTAAAAAAATAAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTA                                                                                                                 <  SRR19973082.2879541/150‑1 (MQ=60)
                                    TATTTCCATAAGTGATTATCCTCATTCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGATGAAAACAATGAAAAAAGGGCCCGCAGGACCTTTTTTCGATATAAATAGAGATTAGAACTCTCAAACCAGACCCAGAGCTAA                                                                                                                >  SRR19973082.3369767/1‑150 (MQ=60)
                                     attacaatccgttttgagcctcaggctaccgggcgaaagatcacacaaaaaaagtgtttttgaacgctcaaaaaaattAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACG                                                                                                               <  SRR19973082.846008/72‑1 (MQ=60)
                                     ATTTCCATACGTGATTATCCTCATGCCAACGGTCTAAATATAACACAAAAAAACTTTGTTTTTACGCTCAAAAAATTCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACG                                                                                                               <  SRR19973082.1659611/150‑1 (MQ=60)
                                      TTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGA                                                                                                              >  SRR19973082.4478498/1‑150 (MQ=60)
                                        TCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCCAACCAGACCCAGAGCTACGATG                                                                                                            >  SRR19973082.2401901/1‑150 (MQ=60)
                                        TCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATG                                                                                                            >  SRR19973082.2402107/1‑150 (MQ=60)
                                         CCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGT                                                                                                           >  SRR19973082.2433231/1‑150 (MQ=60)
                                         CCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGT                                                                                                           >  SRR19973082.1169057/1‑150 (MQ=60)
                                         CCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGT                                                                                                           >  SRR19973082.4201123/1‑150 (MQ=60)
                                            tcctttataatccacatgcccacggaccaaagataaaacaAAAAAACTGATTTTGTACGATAAAAACAATAAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTAT                                                                                                        <  SRR19973082.2478274/110‑1 (MQ=60)
                                             ACGTTATTATCCTCATGCGACCGGTCGCACGAGTACACCAAAAACCTGCGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATC                                                                                                       <  SRR19973082.611091/150‑1 (MQ=60)
                                               tttATTATCCTCATGCGAACGGTCGCAAGAGTACACAAAAAAACTGATTTTTTCCGCTGAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAG                                                                                                     <  SRR19973082.2215786/147‑1 (MQ=60)
                                               GTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAG                                                                                                     <  SRR19973082.2215619/150‑1 (MQ=60)
                                                     ATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGA                                                                                               >  SRR19973082.42544/1‑150 (MQ=60)
                                                     ATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGA                                                                                               >  SRR19973082.887583/1‑150 (MQ=60)
                                                      TCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTCCGATGTTATCAGTGTTGAT                                                                                              >  SRR19973082.1274759/1‑150 (MQ=60)
                                                      TCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAT                                                                                              >  SRR19973082.3682715/1‑150 (MQ=60)
                                                        CTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGC                                                                                            >  SRR19973082.2893580/1‑150 (MQ=60)
                                                          CATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCA                                                                                          >  SRR19973082.3479825/1‑150 (MQ=60)
                                                           ATGCGAACGGTCGAAAGAGTACACAAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAG                                                                                         <  SRR19973082.2309098/150‑1 (MQ=60)
                                                           ATGCGAACGGTCGAAAGAGTACACAAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAG                                                                                         <  SRR19973082.4685357/150‑1 (MQ=60)
                                                           ATGCGAACGGTCGAAAGAGAACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAG                                                                                         <  SRR19973082.4684544/150‑1 (MQ=60)
                                                               GAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTC                                                                                     >  SRR19973082.155633/1‑150 (MQ=60)
                                                               GAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTC                                                                                     >  SRR19973082.1134459/1‑150 (MQ=60)
                                                                AACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCA                                                                                    >  SRR19973082.3270035/1‑150 (MQ=60)
                                                                AACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTGTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCA                                                                                    >  SRR19973082.2356620/1‑150 (MQ=60)
                                                                  CGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACG                                                                                  >  SRR19973082.1074208/1‑150 (MQ=60)
                                                                       GCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGA                                                                             >  SRR19973082.234773/1‑150 (MQ=60)
                                                                       GCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGA                                                                             >  SRR19973082.533108/1‑150 (MQ=60)
                                                                        CAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAA                                                                            <  SRR19973082.2688424/150‑1 (MQ=60)
                                                                        AAAGAGTACACAAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAA                                                                            <  SRR19973082.2689523/150‑1 (MQ=60)
                                                                         AAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAAC                                                                           >  SRR19973082.4701116/1‑150 (MQ=60)
                                                                           GAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTG                                                                         >  SRR19973082.3077924/1‑150 (MQ=60)
                                                                            acaACACCAAAAAACTTATTTTGAACGATTAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGG                                                                        <  SRR19973082.4500648/147‑1 (MQ=60)
                                                                             taccaaaaaaaaagtgtgtttttacgcgtaaacaatTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGT                                                                       <  SRR19973082.2811687/114‑1 (MQ=60)
                                                                              accaacaaaaaaattattattaccgcaaaaaaaaatcAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTT                                                                      <  SRR19973082.3590981/113‑1 (MQ=60)
                                                                               ACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTG                                                                     >  SRR19973082.1574240/1‑150 (MQ=60)
                                                                                   CAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGT                                                                 >  SRR19973082.3160224/1‑150 (MQ=60)
                                                                                    AAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATAGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATAAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTC                                                                >  SRR19973082.3660960/1‑150 (MQ=60)
                                                                                    AAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTC                                                                >  SRR19973082.520970/1‑150 (MQ=60)
                                                                                    AAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTC                                                                >  SRR19973082.445540/1‑150 (MQ=60)
                                                                                    AAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTC                                                                >  SRR19973082.519992/1‑150 (MQ=60)
                                                                                        AACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATAGAGATTATAACAGGCAAACAAGACCAAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCAAGC                                                            >  SRR19973082.1597743/1‑150 (MQ=60)
                                                                                         ACTGAGTTTGTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCA                                                           <  SRR19973082.3091960/150‑1 (MQ=60)
                                                                                         ACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCA                                                           >  SRR19973082.1771297/1‑150 (MQ=60)
                                                                                         ACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCA                                                           >  SRR19973082.4173026/1‑150 (MQ=60)
                                                                                          CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAG                                                          >  SRR19973082.1707223/1‑150 (MQ=60)
                                                                                           TGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGG                                                         <  SRR19973082.3967510/150‑1 (MQ=60)
                                                                                              GTTTGTCCGCTCAAAACAATCAAAACAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTG                                                      <  SRR19973082.1250934/150‑1 (MQ=60)
                                                                                                   TACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTT                                                 <  SRR19973082.3187976/150‑1 (MQ=60)
                                                                                                       CTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAG                                             <  SRR19973082.3126019/150‑1 (MQ=60)
                                                                                                       CTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAG                                             <  SRR19973082.3125957/150‑1 (MQ=60)
                                                                                                        TTAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGT                                            <  SRR19973082.144720/150‑1 (MQ=60)
                                                                                                        TGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGT                                            <  SRR19973082.144735/150‑1 (MQ=60)
                                                                                                        TGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGGTGATTTTGTAGT                                            >  SRR19973082.4553672/1‑150 (MQ=60)
                                                                                                         GAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTC                                           >  SRR19973082.4182126/1‑150 (MQ=60)
                                                                                                              CAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGT                                      >  SRR19973082.2284193/1‑150 (MQ=60)
                                                                                                                ATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAG                                    <  SRR19973082.1671142/150‑1 (MQ=60)
                                                                                                                ATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAG                                    >  SRR19973082.1633037/1‑150 (MQ=60)
                                                                                                                ATGAAAAAAGGGCCCGCAGGCCCGTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAG                                    >  SRR19973082.535703/1‑150 (MQ=60)
                                                                                                                 TCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGG                                   <  SRR19973082.2842910/150‑1 (MQ=60)
                                                                                                                  GAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGT                                  >  SRR19973082.2590363/1‑150 (MQ=60)
                                                                                                                   AAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTG                                 <  SRR19973082.339098/150‑1 (MQ=60)
                                                                                                                    AAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGG                                <  SRR19973082.803418/150‑1 (MQ=60)
                                                                                                                    AAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGG                                >  SRR19973082.3549498/1‑150 (MQ=60)
                                                                                                                    AAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATGAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGG                                <  SRR19973082.2395621/150‑1 (MQ=60)
                                                                                                                       AAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACA                             >  SRR19973082.2657866/1‑150 (MQ=60)
                                                                                                                        AGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT                            <  SRR19973082.4276941/150‑1 (MQ=60)
                                                                                                                        AGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT                            <  SRR19973082.4276800/150‑1 (MQ=60)
                                                                                                                         GGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATG                           >  SRR19973082.3374213/1‑150 (MQ=60)
                                                                                                                         GGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATG                           >  SRR19973082.981686/1‑150 (MQ=60)
                                                                                                                          GGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATGAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGT                          <  SRR19973082.3280313/150‑1 (MQ=60)
                                                                                                                               GCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTG                     >  SRR19973082.4138188/1‑150 (MQ=60)
                                                                                                                                 AGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTT                   >  SRR19973082.2860429/1‑150 (MQ=60)
                                                                                                                                   GCCCTTTGTTCGATATAAATCGAGATGAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGA                 <  SRR19973082.4774858/150‑1 (MQ=60)
                                                                                                                                    CCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAA                <  SRR19973082.4226794/150‑1 (MQ=60)
                                                                                                                                    CCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAA                <  SRR19973082.4228985/150‑1 (MQ=60)
                                                                                                                                      CTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGT              >  SRR19973082.3477980/1‑150 (MQ=60)
                                                                                                                                                TATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGC    >  SRR19973082.3650954/1‑150 (MQ=60)
                                                                                                                                                 ATAAATCGAGATTATAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTATGTGGACATGTTTTTGTTGAAGTAGTATGTAGCA   >  SRR19973082.1906593/1‑150 (MQ=60)
                                                                                                                                                 ATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCA   >  SRR19973082.2790450/1‑150 (MQ=60)
                                                                                                                                                 ATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCA   >  SRR19973082.1906610/1‑150 (MQ=60)
                                                                                                                                                 ATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCA   >  SRR19973082.1906784/1‑150 (MQ=60)
                                                                                                                                                  TAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAC  <  SRR19973082.2006227/150‑1 (MQ=60)
                                                                                                                                                  TAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAC  <  SRR19973082.2007189/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                    
GAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAC  >  CP009273/2304969‑2305264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑