Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,305,116 C→A 90.1% intergenic (‑103/+9) apbE ← / ← ompC putative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,305,1160CA90.1% 626.6 / 57.4 220intergenic (‑103/+9)apbE/ompCputative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base C (16/6);  new base A (102/96);  total (118/102)
Fisher's exact test for biased strand distribution p-value = 7.20e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 223 total aligned reads displayed.

GAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGC  >  CP009273/2304969‑2305262
                                                                                                                                                   |                                                                                                                                                  
 agCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAAt                                                                                                                                                 <  2:3752630/150‑1 (MQ=255)
  gcgcGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCTAACGGTCGCAAGATTACACCAAAAAACTGAGTTTTTACGCTCAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATc                                                                                                                                                <  1:2374757/150‑1 (MQ=255)
      ggCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGCTATAAATCgaga                                                                                                                                            >  2:2371348/1‑150 (MQ=255)
      ggCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCgaga                                                                                                                                            >  2:2673399/1‑150 (MQ=255)
        cagccagcAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACAGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGAtt                                                                                                                                          >  1:3977328/1‑150 (MQ=255)
         cgccagcTGTGCCACGGGGGTAATGTTTATTCCAATCCGTTATTATCCTCTTGCTAACGGTCGAAAGAGACCACAAAAAACCTGTTTTTGTACGCTTAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTa                                                                                                                                         <  1:515640/149‑1 (MQ=255)
         agccagcAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTa                                                                                                                                         >  1:1511921/1‑150 (MQ=255)
         agccagcAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTGTGTTCGATATAAATCGAGATTa                                                                                                                                         >  2:703805/1‑150 (MQ=255)
          gccagcAGTGCCACGCGGGTAACGCTTATTTCCATCCGTGATGATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAg                                                                                                                                        <  2:3134908/150‑1 (MQ=255)
          gccagcAGTGCCACGCGGGTAAAGCTTATTTCAATACGTGATTATCCTCATGCTAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAAAAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAg                                                                                                                                        <  1:2820941/150‑1 (MQ=255)
          gccagcAGTGCCACGCGGGTAAAGCTTATGTCCATACGTGATTAGCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAg                                                                                                                                        <  2:3134885/150‑1 (MQ=255)
             agcagTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAAc                                                                                                                                     >  1:177836/1‑150 (MQ=255)
             agcagTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAAc                                                                                                                                     >  2:3544450/1‑150 (MQ=255)
              gcagTGCCACGCGGGTAAAGCTTAGTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACt                                                                                                                                    <  2:3865877/150‑1 (MQ=255)
              gcagTGACACGCGGGTAAAGCTTATTTCCATACGTTATTATCCTCATGCGAACGGTCGCAAGAGTACACAAAAAAACTGAGTTTTTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACt                                                                                                                                    <  2:1230426/150‑1 (MQ=255)
               cagTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTAGCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTg                                                                                                                                   <  2:2144135/150‑1 (MQ=255)
                agTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTgg                                                                                                                                  >  1:1591316/1‑150 (MQ=255)
                    ccACGGGGTTAATGCTTATTTCCATCCGTGATTATCCTCATGCGAAGGGTCGCATGAGTACACCAAAAAACTGCGTTTGTACGCTTAAAACATTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCaaa                                                                                                                              <  1:1906477/150‑1 (MQ=255)
                       ggggggTAAAGCTTATTTCAATACGTGTTTATCCTCATGCTAACGGTCGAAAGAGTACACCAAAAAACTGTTTTTGTACGCTTAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCa                                                                                                                           <  1:4255138/147‑1 (MQ=255)
                       cgcgGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAAATGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTGTGTTCGATATCAATCGAGATTAGAACTGGTAAACCa                                                                                                                           >  1:3665986/1‑150 (MQ=255)
                           ggTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGAcc                                                                                                                       >  2:1654394/1‑150 (MQ=255)
                            gTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGAccc                                                                                                                      >  1:184245/1‑150 (MQ=255)
                               aaGCTTATTTCCATACGTGATTATCCTCTTGCGAACGGTCGCAAGAGTACACCAAAAACCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCaga                                                                                                                   <  1:4270680/150‑1 (MQ=255)
                                  cTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCt                                                                                                                >  2:3777777/1‑150 (MQ=255)
                                    tATTTCCATACGTGATTATCCTCATGCGAACGGTCTAATGATTACACCAAAAACCTGAGTTTTTACGATTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTAc                                                                                                              <  1:3946823/150‑1 (MQ=255)
                                    tATTTCAATACGTTATTATCCTCATGCGAACGGTCGAAAGAGTACACCAAAAAACTGAGTTTGTACGCTTAAAAAAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTAc                                                                                                              <  1:4198300/150‑1 (MQ=255)
                                     aTTTCCATACGTGATTATCCTCATGCTAACGGTCGCAAGAGTACACCAAAAAACTTAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACg                                                                                                             <  1:3608000/150‑1 (MQ=255)
                                     aTTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACg                                                                                                             >  2:3262782/1‑150 (MQ=255)
                                         ccATACGTGATTATCCTCATGCTACCGGTCGCAAGAGTACACCAAAAAACTGTGTTTGTACGCTTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGt                                                                                                         <  2:3043847/150‑1 (MQ=255)
                                         ccATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGt                                                                                                         >  2:79871/1‑150 (MQ=255)
                                          cATACGTGATTATCCTCATGCGAACGGTCGAAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGtt                                                                                                        <  1:670808/150‑1 (MQ=255)
                                               gTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAg                                                                                                   >  1:2335525/1‑150 (MQ=255)
                                                 gATTATCCTCATGCGAACGGTCGAACGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAgtg                                                                                                 <  1:1006065/150‑1 (MQ=255)
                                                  aTTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAgtgt                                                                                                >  2:632430/1‑150 (MQ=255)
                                                   ttATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGtt                                                                                               >  1:2090810/1‑150 (MQ=255)
                                                     aTCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGa                                                                                             <  1:991193/150‑1 (MQ=255)
                                                     aTCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGa                                                                                             >  1:810956/1‑150 (MQ=255)
                                                      tCCTCATGCGACCGGCCGCAAGAGTACACAAAAAAACTGTTTTTTTACGCTTAAAAAAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAt                                                                                            <  1:1179407/150‑1 (MQ=255)
                                                      tCCTCATGCGAACGGTCGAAAGAGTACACAAAAAAACGGAGTTTTTACGCTCAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAt                                                                                            <  1:1179450/150‑1 (MQ=255)
                                                       ccTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATg                                                                                           >  2:226479/1‑150 (MQ=255)
                                                         tCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGcc                                                                                         <  2:184245/150‑1 (MQ=255)
                                                         tCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGcc                                                                                         >  1:975465/1‑150 (MQ=255)
                                                           aTGCGAACGGTCGCAAGAGTACACCAAAAAACGGCGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAg                                                                                       <  2:1404806/150‑1 (MQ=255)
                                                               gAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTc                                                                                   >  2:3414341/1‑150 (MQ=255)
                                                                aaCGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCa                                                                                  >  1:1882115/1‑150 (MQ=255)
                                                                aaCGGTCGAAAGAGTACACCAAAAAACTGATTTTGTACGCTTAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCa                                                                                  <  1:3502632/150‑1 (MQ=255)
                                                                     tCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGt                                                                             >  2:505442/1‑150 (MQ=255)
                                                                     tCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGt                                                                             <  1:317339/150‑1 (MQ=255)
                                                                      cGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTg                                                                            >  1:1786135/1‑150 (MQ=255)
                                                                      cGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTg                                                                            >  1:2311541/1‑150 (MQ=255)
                                                                       gCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGa                                                                           >  2:387357/1‑150 (MQ=255)
                                                                          agagTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCCGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCCGCGTCACGAGTGACCt                                                                        >  2:1947602/1‑150 (MQ=255)
                                                                          agagTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACt                                                                        >  2:1947303/1‑150 (MQ=255)
                                                                              tACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGtt                                                                    >  1:2674805/1‑150 (MQ=255)
                                                                               acacCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGACCTGGTTg                                                                   >  2:4061663/1‑150 (MQ=255)
                                                                               acacCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTg                                                                   >  2:3952386/1‑150 (MQ=255)
                                                                                 acCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtc                                                                 <  1:2840867/150‑1 (MQ=255)
                                                                                   cAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcgt                                                               >  2:1713581/1‑150 (MQ=255)
                                                                                     aaaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTcc                                                             >  2:1452282/1‑150 (MQ=255)
                                                                                     aaaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTcc                                                             >  2:2666893/1‑150 (MQ=255)
                                                                                      aaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCca                                                            <  2:3455166/150‑1 (MQ=255)
                                                                                        aaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcagc                                                          >  1:502903/1‑150 (MQ=255)
                                                                                           tGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAgg                                                       >  2:2919383/1‑150 (MQ=255)
                                                                                            gAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGt                                                      >  2:4349394/1‑150 (MQ=255)
                                                                                                 tGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAtt                                                 <  2:133346/150‑1 (MQ=255)
                                                                                                  gTACGCTGAAAACACTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAttt                                                <  2:3570575/150‑1 (MQ=255)
                                                                                                       cTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAg                                           >  1:3022677/1‑150 (MQ=255)
                                                                                                       atGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAg                                           <  1:1936223/149‑1 (MQ=255)
                                                                                                            aaCAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTTATTTTGTAGTCAAc                                      <  1:1605232/150‑1 (MQ=255)
                                                                                                               aaTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTa                                   >  2:3291210/1‑150 (MQ=255)
                                                                                                               aaTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTa                                   <  1:3544450/150‑1 (MQ=255)
                                                                                                                aTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAg                                  <  1:2062394/150‑1 (MQ=255)
                                                                                                                 tGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg                                 >  1:1535231/1‑150 (MQ=255)
                                                                                                                 tGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg                                 >  2:2475663/1‑150 (MQ=255)
                                                                                                                  gAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGt                                >  1:4317198/1‑150 (MQ=255)
                                                                                                                    aaaaaGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTgg                              <  2:1157382/150‑1 (MQ=255)
                                                                                                                    aaaaaGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTgg                              >  2:448416/1‑150 (MQ=255)
                                                                                                                    aaaaaGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTgg                              <  1:1453437/150‑1 (MQ=255)
                                                                                                                     aaaaGGGCCCGCCGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGGTGATTTTGTAGTCAACGTAGGTGGa                             >  1:3420466/1‑150 (MQ=255)
                                                                                                                     aaaaGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGa                             >  1:644144/1‑150 (MQ=255)
                                                                                                                     aaaaGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGa                             >  1:2815552/1‑150 (MQ=255)
                                                                                                                     aaaaGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGa                             >  1:1340757/1‑150 (MQ=255)
                                                                                                                      aaaGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGTTGTAc                            >  2:1729443/1‑150 (MQ=255)
                                                                                                                      aaaGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGAc                            <  1:2951271/150‑1 (MQ=255)
                                                                                                                        aGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt                          >  1:3654174/1‑150 (MQ=255)
                                                                                                                        aGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt                          <  1:1033912/150‑1 (MQ=255)
                                                                                                                           gCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtt                       <  2:1024255/150‑1 (MQ=255)
                                                                                                                              cGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttttt                    >  2:1712777/1‑150 (MQ=255)
                                                                                                                               gCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttg                   >  1:2676711/1‑150 (MQ=255)
                                                                                                                                cAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt                  >  2:899516/1‑150 (MQ=255)
                                                                                                                                cAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt                  >  2:1372419/1‑150 (MQ=255)
                                                                                                                                 aGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACTCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgtt                 <  2:2311714/150‑1 (MQ=255)
                                                                                                                                    cccTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGaa              <  2:1789715/150‑1 (MQ=255)
                                                                                                                                     ccTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag             >  1:2237732/1‑150 (MQ=255)
                                                                                                                                       tttGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagta           >  1:2132725/1‑150 (MQ=255)
                                                                                                                                       tttGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagta           <  1:1344279/150‑1 (MQ=255)
                                                                                                                                            tCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg      <  2:2832724/150‑1 (MQ=255)
                                                                                                                                            tCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg      >  1:3807753/1‑150 (MQ=255)
                                                                                                                                            tCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg      <  1:1562574/150‑1 (MQ=255)
                                                                                                                                               atatCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag   >  1:76877/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGC  >  CP009273/2304969‑2305262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACC  >  CP009273/2304968‑2305265
                                                                                                                                                    |                                                                                                                                                     
AGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAA                                                                                                                                                      >  SRR19973079.3237905/1‑150 (MQ=60)
   GCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCAATACGTTATTATCCTCATGCTAACGGTCGCAAGATTACACCAAAAAACTGAGTTTTTACGCTCAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATC                                                                                                                                                   <  SRR19973079.2387052/150‑1 (MQ=60)
         CAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACAGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATT                                                                                                                                             >  SRR19973079.3997324/1‑150 (MQ=60)
          CGCCAGCTGTGCCACGGGGGTAATGTTTATTCCAATCCGTTATTATCCTCTTGCTAACGGTCGAAAGAGACCACAAAAAACCTGTTTTTGTACGCTTAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTA                                                                                                                                            <  SRR19973079.518758/150‑1 (MQ=36)
          AGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTA                                                                                                                                            >  SRR19973079.1520257/1‑150 (MQ=60)
           GCCAGCAGTGCCACGCGGGTAAAGCTTATTTCAATACGTGATTATCCTCATGCTAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAAAAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAG                                                                                                                                           <  SRR19973079.2835365/150‑1 (MQ=60)
            ccatcagtgccagggggttaaagcttttttcattaggttATTATCCTCATGCGAAGGGTCGCATGTGTAAACAAAAAAACTGATTTTGTCCGCTTAAAAAAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGA                                                                                                                                          <  SRR19973079.3165613/111‑1 (MQ=60)
              AGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAAC                                                                                                                                        >  SRR19973079.179162/1‑150 (MQ=60)
                 AGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATATATCGAGATTAGAACTGG                                                                                                                                     >  SRR19973079.2735515/1‑150 (MQ=60)
                 AGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGG                                                                                                                                     >  SRR19973079.1600077/1‑150 (MQ=60)
                     CCACGGGGTTAATGCTTATTTCCATCCGTGATTATCCTCATGCGAAGGGTCGCATGAGTACACCAAAAAACTGCGTTTGTACGCTTAAAACATTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAA                                                                                                                                 <  SRR19973079.1916637/150‑1 (MQ=60)
                       agggggttaaagtttttttacatagtttattaccctattgctaaggttctaatgattacacaaaaaaaatttttttgtaggtttaaaaaaataAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACC                                                                                                                               <  SRR19973079.3051721/57‑1 (MQ=60)
                        gggGGGTAAAGCTTATTTCAATACGTGTTTATCCTCATGCTAACGGTCGAAAGAGTACACCAAAAAACTGTTTTTGTACGCTTAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCA                                                                                                                              <  SRR19973079.4276663/147‑1 (MQ=60)
                        CGCGGTTAAAGATTATTACCATACTTTATTATCCTCATGCTAACGTTCGCAAGATTACACAAAAAAAATTTTTTTGTACGCTGAAAAAATTCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCA                                                                                                                              <  SRR19973079.1540139/150‑1 (MQ=53)
                        CGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAAATGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTGTGTTCGATATCAATCGAGATTAGAACTGGTAAACCA                                                                                                                              >  SRR19973079.3684655/1‑150 (MQ=60)
                             GTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCC                                                                                                                         >  SRR19973079.185597/1‑150 (MQ=60)
                             GTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCC                                                                                                                         >  SRR19973079.185572/1‑150 (MQ=60)
                                AAGCTTATTTCCATACGTGATTATCCTCTTGCGAACGGTCGCAAGAGTACACCAAAAACCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGA                                                                                                                      <  SRR19973079.4292273/150‑1 (MQ=60)
                                 tgtttttttccttcggtttTTATCCTCTTGCGAAGGGTCGCAAGTGTACACCAAAAAAGTGTGTTTGTCCGCTTAAAAAATTAAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAG                                                                                                                     <  SRR19973079.3339137/131‑1 (MQ=60)
                                 cttttattacaatacgtcataacccaccgcctaacggccccaagataacacaaaaaaaatgctttcgcacgataaaaaaaatcAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAG                                                                                                                     <  SRR19973079.2948210/67‑1 (MQ=60)
                                 atttttttaaaatccgtttttatcctcttgccaagggtcccaatactacaaaaaaaaaattagttttaactcttaaaaaaatcAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAG                                                                                                                     <  SRR19973079.765359/67‑1 (MQ=60)
                                 acataattcccagacgttattagCCTCATGCGAACGGACGCAAGACAACACCAAAAAACTTAGTTTTTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAG                                                                                                                     <  SRR19973079.1167549/127‑1 (MQ=60)
                                 AGCTTATTTCCATCCGTGATTATCCTCATGCGAACGGTCGCAAGAGAACACAAAAAAACTGTGTTGGTACGATTAAAAAAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAG                                                                                                                     <  SRR19973079.512101/150‑1 (MQ=60)
                                 AGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAG                                                                                                                     >  SRR19973079.355757/1‑150 (MQ=60)
                                   CTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCT                                                                                                                   >  SRR19973079.1462591/1‑150 (MQ=60)
                                     TATTTCCATACGTGATTATCCTCATGCGAACGGTCTAATGATTACACCAAAAACCTGAGTTTTTACGATTAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTAC                                                                                                                 <  SRR19973079.3966732/150‑1 (MQ=60)
                                     TATTTCAATACGTTATTATCCTCATGCGAACGGTCGAAAGAGTACACCAAAAAACTGAGTTTGTACGCTTAAAAAAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTAC                                                                                                                 <  SRR19973079.4219575/150‑1 (MQ=60)
                                      ATTTCCATACGTGATTATCCTCATGCTAACGGTCGCAAGAGTACACCAAAAAACTTAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACG                                                                                                                <  SRR19973079.3626298/150‑1 (MQ=60)
                                          CCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGT                                                                                                            >  SRR19973079.10011/1‑150 (MQ=60)
                                           CATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTT                                                                                                           >  SRR19973079.2351386/1‑150 (MQ=60)
                                           CATACGTGATTATCCTCATGCGAACGGTCGAAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTT                                                                                                           <  SRR19973079.674529/150‑1 (MQ=60)
                                            ATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTA                                                                                                          >  SRR19973079.343334/1‑150 (MQ=60)
                                             TACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTAT                                                                                                         >  SRR19973079.531807/1‑150 (MQ=60)
                                                GTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAG                                                                                                      >  SRR19973079.2482157/1‑150 (MQ=60)
                                                GTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAG                                                                                                      >  SRR19973079.2347656/1‑150 (MQ=60)
                                                  GATTATCCTCATGCGAACGGTCGAACGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTG                                                                                                    <  SRR19973079.1011735/150‑1 (MQ=60)
                                                    TTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTT                                                                                                  >  SRR19973079.2101702/1‑150 (MQ=60)
                                                     TATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTG                                                                                                 >  SRR19973079.1983979/1‑150 (MQ=60)
                                                      ATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACTCAGAGCTACGATGTTATCAGTGTTGA                                                                                                >  SRR19973079.2323711/1‑150 (MQ=60)
                                                      ATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGA                                                                                                >  SRR19973079.2836203/1‑150 (MQ=60)
                                                      ATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGA                                                                                                >  SRR19973079.3001065/1‑150 (MQ=60)
                                                      ATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGA                                                                                                >  SRR19973079.2835195/1‑150 (MQ=60)
                                                      ATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGA                                                                                                >  SRR19973079.815603/1‑150 (MQ=60)
                                                      ATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGA                                                                                                <  SRR19973079.996290/150‑1 (MQ=60)
                                                      AACCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTTAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGA                                                                                                <  SRR19973079.996796/150‑1 (MQ=60)
                                                       tcctcccgctAACGGTCGAAAGAGAACACCAAAAAACTGATTTGGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAT                                                                                               <  SRR19973079.1186222/140‑1 (MQ=60)
                                                       TCCTCATGCTAACGGTCGCAAGATAACACAAAAAAACTGTTTTTGTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAT                                                                                               <  SRR19973079.1186420/150‑1 (MQ=60)
                                                       TCCTCATGCGACCGGCCGCAAGAGTACACAAAAAAACTGTTTTTTTACGCTTAAAAAAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAT                                                                                               <  SRR19973079.1186196/150‑1 (MQ=60)
                                                       TCCTCATGCGAACGGTCGAAAGAGTACACAAAAAAACGGAGTTTTTACGCTCAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAT                                                                                               <  SRR19973079.1186239/150‑1 (MQ=60)
                                                        CCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATG                                                                                              >  SRR19973079.3025/1‑150 (MQ=60)
                                                         gtcatgcgaacgttcgcaatattaaaaaaaaaaacttattttttacgattAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGC                                                                                             <  SRR19973079.2708955/100‑1 (MQ=60)
                                                          TCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCC                                                                                            >  SRR19973079.981004/1‑150 (MQ=60)
                                                                GAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTC                                                                                      >  SRR19973079.938357/1‑150 (MQ=60)
                                                                GAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTC                                                                                      >  SRR19973079.801159/1‑150 (MQ=60)
                                                                GAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTC                                                                                      >  SRR19973079.367525/1‑150 (MQ=60)
                                                                 ACCGGTCGCAAGAGAACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCA                                                                                     <  SRR19973079.4283805/150‑1 (MQ=60)
                                                                 AACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCA                                                                                     <  SRR19973079.4283686/150‑1 (MQ=60)
                                                                 AACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCA                                                                                     >  SRR19973079.1892125/1‑150 (MQ=60)
                                                                 AACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCA                                                                                     <  SRR19973079.3520652/150‑1 (MQ=60)
                                                                 AACGGTCGAAAGAGTACACCAAAAAACTGATTTTGTACGCTTAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCA                                                                                     <  SRR19973079.3520643/150‑1 (MQ=60)
                                                                  ACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCAC                                                                                    >  SRR19973079.3579895/1‑150 (MQ=60)
                                                                     gtcccaatattccccaAAAAAAGTGAGTTTGTACGCTCAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAG                                                                                 <  SRR19973079.4053906/134‑1 (MQ=60)
                                                                      TCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGT                                                                                <  SRR19973079.319161/150‑1 (MQ=60)
                                                                       CGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTG                                                                               >  SRR19973079.2323538/1‑150 (MQ=60)
                                                                       CGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTG                                                                               >  SRR19973079.1795367/1‑150 (MQ=60)
                                                                       CGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTG                                                                               >  SRR19973079.1795471/1‑150 (MQ=60)
                                                                            ttgaaacaaaaaaaagttAGTTTGTACGCTCAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTTTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTG                                                                          <  SRR19973079.2990965/132‑1 (MQ=60)
                                                                               TACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTT                                                                       >  SRR19973079.2688673/1‑150 (MQ=60)
                                                                               TACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTT                                                                       >  SRR19973079.2688672/1‑150 (MQ=60)
                                                                               AACACAAAAAAACTGAGTTTGTACGCTTAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTT                                                                       <  SRR19973079.1595284/150‑1 (MQ=60)
                                                                                ACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTG                                                                      <  SRR19973079.3922451/150‑1 (MQ=60)
                                                                                  ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTC                                                                    <  SRR19973079.2855348/150‑1 (MQ=60)
                                                                                  ACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTC                                                                    <  SRR19973079.3962917/150‑1 (MQ=60)
                                                                                         AACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGC                                                             >  SRR19973079.505957/1‑150 (MQ=60)
                                                                                           cttttttttTACGCTTAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAG                                                           <  SRR19973079.753202/141‑1 (MQ=60)
                                                                                           CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAG                                                           >  SRR19973079.1891497/1‑150 (MQ=60)
                                                                                           CTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAG                                                           >  SRR19973079.2179670/1‑150 (MQ=60)
                                                                                            TGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGG                                                          >  SRR19973079.528786/1‑150 (MQ=60)
                                                                                              AGTTTGTACGCTGAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTT                                                        <  SRR19973079.1929960/150‑1 (MQ=60)
                                                                                                  TGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATT                                                    >  SRR19973079.160712/1‑150 (MQ=60)
                                                                                                        CTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAG                                              >  SRR19973079.3038308/1‑150 (MQ=60)
                                                                                                        CTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAG                                              <  SRR19973079.1947133/150‑1 (MQ=60)
                                                                                                        ATGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAG                                              <  SRR19973079.1946555/150‑1 (MQ=60)
                                                                                                         TGAAAAAAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGT                                             <  SRR19973079.3719003/150‑1 (MQ=60)
                                                                                                           AAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGGTGATTTTGTAGTCA                                           >  SRR19973079.1456827/1‑150 (MQ=60)
                                                                                                            AAAAAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAA                                          <  SRR19973079.1547490/150‑1 (MQ=60)
                                                                                                             AACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAAC                                         <  SRR19973079.3498625/150‑1 (MQ=60)
                                                                                                             AACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTTATTTTGTAGTCAAC                                         <  SRR19973079.1614042/150‑1 (MQ=60)
                                                                                                               CAATGAAAAAAGGGCCCGCGGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGAAGGTTGTGTTTGTAGTCAACGT                                       >  SRR19973079.477972/1‑150 (MQ=60)
                                                                                                               CAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGT                                       <  SRR19973079.1892273/150‑1 (MQ=60)
                                                                                                               CAATGAAAAAAGGGCCCGCAGGCCCGTTGTTCGATATAAAACGGGATTAGAACTGGGAAACCCCACCCAGAGCTACGAAGGTTTCAGTGTTGTTGGCCGCGTCACGGGTGTACGGGGTTTCGTCCCGCAGGGTGATTTTGGAGTCCACGT                                       >  SRR19973079.478246/1‑150 (MQ=28)
                                                                                                                AATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTA                                      <  SRR19973079.3562603/150‑1 (MQ=60)
                                                                                                                 ATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAG                                     <  SRR19973079.2073197/150‑1 (MQ=60)
                                                                                                                 ATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAG                                     <  SRR19973079.2074274/150‑1 (MQ=60)
                                                                                                                  TGAAAAAAGGGCCCGCCGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGG                                    <  SRR19973079.1417910/150‑1 (MQ=60)
                                                                                                                  TGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGG                                    >  SRR19973079.1543705/1‑150 (MQ=60)
                                                                                                                   GAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGT                                   >  SRR19973079.4338928/1‑150 (MQ=60)
                                                                                                                    AAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTG                                  >  SRR19973079.1812900/1‑150 (MQ=60)
                                                                                                                     AAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGG                                 <  SRR19973079.1461322/150‑1 (MQ=60)
                                                                                                                     AAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGG                                 >  SRR19973079.3161921/1‑150 (MQ=60)
                                                                                                                      AAAAGGGCCCGCCGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGGTGATTTTGTAGTCAACGTAGGTGGA                                >  SRR19973079.3438126/1‑150 (MQ=60)
                                                                                                                      AAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGA                                <  SRR19973079.4146110/150‑1 (MQ=60)
                                                                                                                      AAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGA                                >  SRR19973079.1348276/1‑150 (MQ=60)
                                                                                                                      AAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGA                                >  SRR19973079.648116/1‑150 (MQ=60)
                                                                                                                      AAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGA                                >  SRR19973079.647794/1‑150 (MQ=60)
                                                                                                                      AAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGA                                >  SRR19973079.2829956/1‑150 (MQ=60)
                                                                                                                       AACGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGAC                               <  SRR19973079.2963426/150‑1 (MQ=60)
                                                                                                                       AAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGAC                               <  SRR19973079.3995222/150‑1 (MQ=60)
                                                                                                                       AAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGAC                               <  SRR19973079.2966412/150‑1 (MQ=60)
                                                                                                                         AGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT                             <  SRR19973079.1039569/150‑1 (MQ=60)
                                                                                                                         AGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT                             <  SRR19973079.1039650/150‑1 (MQ=60)
                                                                                                                         AGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAT                             >  SRR19973079.3672770/1‑150 (MQ=60)
                                                                                                                             CCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTT                         >  SRR19973079.4234231/1‑150 (MQ=60)
                                                                                                                                GCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTG                      >  SRR19973079.2690586/1‑150 (MQ=60)
                                                                                                                                 CAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGT                     <  SRR19973079.2283754/150‑1 (MQ=60)
                                                                                                                                 CAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGT                     <  SRR19973079.2283756/150‑1 (MQ=60)
                                                                                                                                   GGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTG                   >  SRR19973079.3664718/1‑150 (MQ=60)
                                                                                                                                      CCTTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAG                >  SRR19973079.2249315/1‑150 (MQ=60)
                                                                                                                                        TTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTA              <  SRR19973079.1722735/150‑1 (MQ=60)
                                                                                                                                        TTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTA              <  SRR19973079.1351286/150‑1 (MQ=60)
                                                                                                                                        TTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTA              <  SRR19973079.1351643/150‑1 (MQ=60)
                                                                                                                                        TTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTA              >  SRR19973079.2143726/1‑150 (MQ=60)
                                                                                                                                        TTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTA              >  SRR19973079.2143238/1‑150 (MQ=60)
                                                                                                                                        TTTGTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTA              <  SRR19973079.1351816/150‑1 (MQ=60)
                                                                                                                                           GTTCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTA           >  SRR19973079.4306389/1‑150 (MQ=60)
                                                                                                                                             TCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGG         <  SRR19973079.1571194/150‑1 (MQ=60)
                                                                                                                                             TCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGG         <  SRR19973079.1570557/150‑1 (MQ=60)
                                                                                                                                             TCGATATAAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGG         >  SRR19973079.3827240/1‑150 (MQ=60)
                                                                                                                                                ATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAG      >  SRR19973079.77598/1‑150 (MQ=60)
                                                                                                                                                    AAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACC  >  SRR19973079.314732/1‑150 (MQ=60)
                                                                                                                                                    AAATCGAGATTAGAACTGGCAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACC  >  SRR19973079.1015645/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
AGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACC  >  CP009273/2304968‑2305265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑