Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 652,597 C→T 100% intergenic (+24/‑151) pagP → / → cspE phospholipid:lipid A palmitoyltransferase/constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273652,5970CT98.3% 589.9 / 0.3 179intergenic (+24/‑151)pagP/cspEphospholipid:lipid A palmitoyltransferase/constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (91/85);  total (92/87)
Fisher's exact test for biased strand distribution p-value = 6.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 179 total aligned reads displayed.

CCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGT  >  CP009273/652454‑652740
                                                                                                                                               |                                                                                                                                               
ccGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTa                                                                                                                                             <  2:2524561/148‑1 (MQ=255)
ccGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTa                                                                                                                                             >  2:673464/1‑148 (MQ=255)
ccGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTGTTCAGATGACCTACATTCCAGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTa                                                                                                                                             <  1:1829601/148‑1 (MQ=255)
  ggTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTaaa                                                                                                                                           <  2:3194247/148‑1 (MQ=255)
   gTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCCATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTaaaa                                                                                                                                          >  1:3293841/1‑148 (MQ=255)
    ttCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAAt                                                                                                                                         >  1:437350/1‑148 (MQ=255)
     tCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATc                                                                                                                                        >  2:1407102/1‑148 (MQ=255)
          tGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAg                                                                                                                                   <  1:3174341/148‑1 (MQ=255)
           gCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGc                                                                                                                                  >  1:689220/1‑148 (MQ=255)
           gCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGc                                                                                                                                  <  1:420924/148‑1 (MQ=255)
           gCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGc                                                                                                                                  <  1:3546544/148‑1 (MQ=255)
             cATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTa                                                                                                                                <  2:890443/148‑1 (MQ=255)
             cATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTa                                                                                                                                <  2:889957/148‑1 (MQ=255)
              aTTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTaa                                                                                                                               >  1:2393408/1‑148 (MQ=255)
               ttGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTaaa                                                                                                                              >  2:350718/1‑148 (MQ=255)
               ttGGCCTCCGTGGGTGATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTaaa                                                                                                                              <  2:3251277/148‑1 (MQ=255)
                  gCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATa                                                                                                                           >  1:1457500/1‑148 (MQ=255)
                   ccTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATaa                                                                                                                          >  1:154221/1‑148 (MQ=255)
                   ccTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATaa                                                                                                                          >  1:1147680/1‑148 (MQ=255)
                      ccGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTa                                                                                                                       >  1:1028949/1‑148 (MQ=255)
                       cGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTaa                                                                                                                      <  1:2983978/148‑1 (MQ=255)
                           ggTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATc                                                                                                                  >  1:1352120/1‑148 (MQ=255)
                              tATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCaaa                                                                                                               >  1:964363/1‑148 (MQ=255)
                              tATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCaaa                                                                                                               <  2:2102069/148‑1 (MQ=255)
                               aTGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCaaaa                                                                                                              <  2:130293/148‑1 (MQ=255)
                                 ggCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAAt                                                                                                            <  2:1697959/148‑1 (MQ=255)
                                 ggCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAAt                                                                                                            >  1:2831290/1‑148 (MQ=255)
                                   cccAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATaa                                                                                                          >  1:2475544/1‑148 (MQ=255)
                                   cccAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATaa                                                                                                          >  1:3259912/1‑148 (MQ=255)
                                      aGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAActc                                                                                                       >  1:79900/1‑146 (MQ=255)
                                      aGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAAcgc                                                                                                       >  2:1610184/1‑148 (MQ=255)
                                       gTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAAcgcg                                                                                                      <  2:673707/148‑1 (MQ=255)
                                        tGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGa                                                                                                     >  1:3026674/1‑148 (MQ=255)
                                         gACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGAc                                                                                                    >  1:3522767/1‑148 (MQ=255)
                                           cTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACtt                                                                                                  <  2:1145441/148‑1 (MQ=255)
                                            ttttCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACttt                                                                                                 <  2:816802/148‑1 (MQ=255)
                                            ttttCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACttt                                                                                                 >  2:631229/1‑148 (MQ=255)
                                                   aTGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACt                                                                                          <  1:323933/148‑1 (MQ=255)
                                                        cTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTa                                                                                     <  1:996503/148‑1 (MQ=255)
                                                              tCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAg                                                                               >  1:1320957/1‑148 (MQ=255)
                                                              tCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACGTTTTAGTAAAg                                                                               >  2:2346543/1‑148 (MQ=255)
                                                                  ggTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAAAATCAAAAATAAAGCGACTTTTATCACTTTTTAGTAAAGTTac                                                                           >  2:2938414/1‑148 (MQ=255)
                                                                   gTACCTACAACAAGGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTaca                                                                          <  1:1626772/148‑1 (MQ=255)
                                                                      ccTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTg                                                                       >  1:2508947/1‑148 (MQ=255)
                                                                       cTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTgg                                                                      <  2:1922247/148‑1 (MQ=255)
                                                                       cTACAACAATCGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTgg                                                                      >  1:3344443/1‑148 (MQ=255)
                                                                       cTACAACAATCGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTgg                                                                      >  1:3344425/1‑148 (MQ=255)
                                                                        tACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGa                                                                     <  2:3310301/148‑1 (MQ=255)
                                                                        tACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGa                                                                     <  2:1548480/148‑1 (MQ=255)
                                                                            acaaTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACaaa                                                                 >  2:965527/1‑148 (MQ=255)
                                                                             caaTGGCAATGTGTACTTGGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAg                                                                <  2:3176201/148‑1 (MQ=255)
                                                                               aTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCg                                                              <  2:2433791/148‑1 (MQ=255)
                                                                               aTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCg                                                              <  2:489581/148‑1 (MQ=255)
                                                                                tGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGt                                                             >  1:1015997/1‑148 (MQ=255)
                                                                                tGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGt                                                             >  1:1016022/1‑148 (MQ=255)
                                                                                tGGCAATGTGTACTGTGCCTGGATGCGCTTTCATTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGt                                                             <  1:2268879/148‑1 (MQ=255)
                                                                                 ggCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTGTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTa                                                            >  2:640037/1‑148 (MQ=255)
                                                                                         gtACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAAtt                                                    >  1:252477/1‑148 (MQ=255)
                                                                                          tACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTg                                                   <  1:2218254/148‑1 (MQ=255)
                                                                                          tACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTg                                                   >  2:3115952/1‑148 (MQ=255)
                                                                                          tACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAAACCGAATTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACAACAATTg                                                   >  1:1836460/1‑148 (MQ=255)
                                                                                           aCTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTgg                                                  <  2:807627/148‑1 (MQ=255)
                                                                                           aCTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTgg                                                  <  1:3434748/148‑1 (MQ=255)
                                                                                           aCTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTgg                                                  <  2:2591047/148‑1 (MQ=255)
                                                                                            cTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGgt                                                 >  2:2889313/1‑148 (MQ=255)
                                                                                              ttGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGgtgt                                               <  1:2957453/148‑1 (MQ=255)
                                                                                               tGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTa                                              <  1:2787196/148‑1 (MQ=255)
                                                                                                  cTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTg                                           <  1:2620065/148‑1 (MQ=255)
                                                                                                    ggATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGt                                         >  1:11255/1‑148 (MQ=255)
                                                                                                          gcTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGa                                   <  2:1028949/148‑1 (MQ=255)
                                                                                                           cTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGac                                  >  2:2306841/1‑148 (MQ=255)
                                                                                                            tttCAGTTTTGATACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGaca                                 >  1:2825866/1‑148 (MQ=255)
                                                                                                                aGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCAC‑TTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCa                            <  2:2367562/148‑1 (MQ=255)
                                                                                                                  ttttGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCAt                           >  1:1419240/1‑148 (MQ=255)
                                                                                                                  ttttGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCAt                           >  2:48620/1‑148 (MQ=255)
                                                                                                                   tttGATACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCAtt                          >  2:125118/1‑148 (MQ=255)
                                                                                                                   tttGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCAtt                          <  2:2393408/148‑1 (MQ=255)
                                                                                                                    ttGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCAttt                         <  1:767140/148‑1 (MQ=255)
                                                                                                                       agaCAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTtgtg                      <  2:397128/148‑1 (MQ=255)
                                                                                                                       agaCAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTtgtg                      <  2:2607534/148‑1 (MQ=255)
                                                                                                                         aCAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAATTTACACTGGACAAATCGTACCACAATTGGTGTACTGGTAACCGACACAGAATGTGTGTc                    >  1:2453111/1‑148 (MQ=255)
                                                                                                                         aCAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTANCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTc                    >  1:1800855/1‑148 (MQ=255)
                                                                                                                          cAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCt                   <  1:1943936/148‑1 (MQ=255)
                                                                                                                           aaaTGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTa                  >  2:2855231/1‑148 (MQ=255)
                                                                                                                               gAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACCGCAGGTGTGTCTAtttt              >  2:2496748/1‑148 (MQ=255)
                                                                                                                                aaGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAttttt             <  1:2308049/148‑1 (MQ=255)
                                                                                                                                aaGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAttttt             >  1:3346112/1‑148 (MQ=255)
                                                                                                                                 aGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTc            <  1:1253919/148‑1 (MQ=255)
                                                                                                                                    tttAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATg         >  2:706729/1‑148 (MQ=255)
                                                                                                                                    tttAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCAGTTGTGTCTATTTTTCATg         >  2:1301303/1‑148 (MQ=255)
                                                                                                                                     ttAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGt        <  2:2825866/148‑1 (MQ=255)
                                                                                                                                        gTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTaaa     >  2:1868962/1‑148 (MQ=255)
                                                                                                                                        gTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTaaa     >  1:3101832/1‑148 (MQ=255)
                                                                                                                                        gTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATGTGTGTCTATTTTTCATGTaaa     >  1:1192888/1‑148 (MQ=255)
                                                                                                                                         tAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAg    <  2:1332849/148‑1 (MQ=255)
                                                                                                                                         tAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAg    >  1:2678106/1‑148 (MQ=255)
                                                                                                                                          aaCTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAgg   <  2:91305/148‑1 (MQ=255)
                                                                                                                                          aaCTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAgg   <  2:952791/148‑1 (MQ=255)
                                                                                                                                           aCTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGt  <  2:2665719/148‑1 (MQ=255)
                                                                                                                                           aCTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGt  <  2:2138716/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                               
CCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGT  >  CP009273/652454‑652740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTT  >  CP009273/652448‑652746
                                                                                                                                                     |                                                                                                                                                     
CCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAACTTT                                                                                                                                                       >  SRR19973067.92273/1‑150 (MQ=60)
 CTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTT                                                                                                                                                      >  SRR19973067.2624473/1‑150 (MQ=60)
 CTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTATTGAGACAAATGAAGTTTTAGTAACTTTT                                                                                                                                                      >  SRR19973067.2342205/1‑150 (MQ=60)
      CCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAA                                                                                                                                                 >  SRR19973067.1811794/1‑150 (MQ=60)
      CCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTGTTCAGATGACCTACATTCCAGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAA                                                                                                                                                 <  SRR19973067.1842035/150‑1 (MQ=60)
       CGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCCATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAA                                                                                                                                                >  SRR19973067.3314562/1‑150 (MQ=60)
       CGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAA                                                                                                                                                <  SRR19973067.1516142/150‑1 (MQ=60)
        GGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAAT                                                                                                                                               >  SRR19973067.440394/1‑150 (MQ=60)
        GGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAAT                                                                                                                                               >  SRR19973067.440268/1‑150 (MQ=60)
            CTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAAT                                                                                                                                           <  SRR19973067.1823071/150‑1 (MQ=60)
             TACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATA                                                                                                                                          <  SRR19973067.2289864/150‑1 (MQ=60)
              ACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAG                                                                                                                                         >  SRR19973067.2654876/1‑150 (MQ=60)
               CTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGC                                                                                                                                        >  SRR19973067.693966/1‑150 (MQ=60)
               CTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGC                                                                                                                                        <  SRR19973067.1367629/150‑1 (MQ=60)
                TGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCT                                                                                                                                       <  SRR19973067.1241917/150‑1 (MQ=60)
                TGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCT                                                                                                                                       <  SRR19973067.3194432/150‑1 (MQ=60)
                 GCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTA                                                                                                                                      <  SRR19973067.423852/150‑1 (MQ=60)
                 GCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTA                                                                                                                                      <  SRR19973067.422644/150‑1 (MQ=60)
                 GCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTA                                                                                                                                      <  SRR19973067.3568922/150‑1 (MQ=60)
                  CCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAA                                                                                                                                     >  SRR19973067.2408986/1‑150 (MQ=60)
                      TGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATA                                                                                                                                 >  SRR19973067.3245746/1‑150 (MQ=60)
                      TGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATA                                                                                                                                 >  SRR19973067.1467255/1‑150 (MQ=60)
                       GGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAA                                                                                                                                >  SRR19973067.155520/1‑150 (MQ=60)
                       GGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAA                                                                                                                                >  SRR19973067.1155370/1‑150 (MQ=60)
                          CTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTA                                                                                                                             >  SRR19973067.1036030/1‑150 (MQ=60)
                          CTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTA                                                                                                                             <  SRR19973067.2827565/150‑1 (MQ=60)
                          CTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTA                                                                                                                             <  SRR19973067.2798403/150‑1 (MQ=60)
                             CGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACA                                                                                                                          <  SRR19973067.3002836/150‑1 (MQ=60)
                             CGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACA                                                                                                                          <  SRR19973067.3002708/150‑1 (MQ=60)
                             CGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACA                                                                                                                          <  SRR19973067.2415547/150‑1 (MQ=60)
                               TGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATC                                                                                                                        >  SRR19973067.1361362/1‑150 (MQ=60)
                                  GTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAA                                                                                                                     >  SRR19973067.2236594/1‑150 (MQ=60)
                                  GTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAA                                                                                                                     >  SRR19973067.970974/1‑150 (MQ=60)
                                     ATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAAT                                                                                                                  >  SRR19973067.2849486/1‑150 (MQ=60)
                                       GGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAA                                                                                                                >  SRR19973067.3280277/1‑150 (MQ=60)
                                       GGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAA                                                                                                                >  SRR19973067.2491766/1‑150 (MQ=60)
                                       GGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAA                                                                                                                >  SRR19973067.2491820/1‑150 (MQ=60)
                                        GCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAAC                                                                                                               >  SRR19973067.3292152/1‑150 (MQ=60)
                                        GACCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAAC                                                                                                               <  SRR19973067.2570514/150‑1 (MQ=60)
                                          CCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACTC                                                                                                             >  SRR19973067.80789/1‑150 (MQ=60)
                                            AGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGA                                                                                                           >  SRR19973067.3045928/1‑150 (MQ=60)
                                            AGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGA                                                                                                           >  SRR19973067.3045946/1‑150 (MQ=60)
                                             GTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGAC                                                                                                          >  SRR19973067.3545047/1‑150 (MQ=60)
                                              TGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACT                                                                                                         <  SRR19973067.3438236/150‑1 (MQ=60)
                                                      CAGATGACCTACATTCCGGGTACCTACAACACTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCAC                                                                                                 <  SRR19973067.335524/150‑1 (MQ=60)
                                                         ATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTT                                                                                              <  SRR19973067.326138/150‑1 (MQ=60)
                                                         ATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTT                                                                                              <  SRR19973067.326302/150‑1 (MQ=60)
                                                              CTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGT                                                                                         <  SRR19973067.1003404/150‑1 (MQ=60)
                                                              CTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGT                                                                                         <  SRR19973067.1003361/150‑1 (MQ=60)
                                                                  ATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAG                                                                                     >  SRR19973067.1329977/1‑150 (MQ=60)
                                                                  ATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAG                                                                                     >  SRR19973067.1329744/1‑150 (MQ=60)
                                                                         GTACCTACAACAAGGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACT                                                                              <  SRR19973067.1637934/150‑1 (MQ=60)
                                                                          TACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTG                                                                             >  SRR19973067.2525406/1‑150 (MQ=60)
                                                                          TACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTG                                                                             >  SRR19973067.2525246/1‑150 (MQ=60)
                                                                          TACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTG                                                                             >  SRR19973067.3510968/1‑150 (MQ=60)
                                                                           ACCTACAACAATCGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGG                                                                            >  SRR19973067.3365616/1‑150 (MQ=60)
                                                                           ACCTACAACAATCGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGG                                                                            >  SRR19973067.3365634/1‑150 (MQ=60)
                                                                             CTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGAC                                                                          <  SRR19973067.1621243/150‑1 (MQ=60)
                                                                                   CAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCG                                                                    >  SRR19973067.2182431/1‑150 (MQ=60)
                                                                                    AATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGT                                                                   >  SRR19973067.1022971/1‑150 (MQ=60)
                                                                                    AATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGT                                                                   >  SRR19973067.1022996/1‑150 (MQ=60)
                                                                                    AATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGT                                                                   >  SRR19973067.1023350/1‑150 (MQ=60)
                                                                                      TGGCAATGTGTACTGTGCCTGGATGCGCTTTCATTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTAC                                                                 <  SRR19973067.2283914/150‑1 (MQ=60)
                                                                                             GTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATT                                                          >  SRR19973067.254213/1‑150 (MQ=60)
                                                                                              TGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAAACCGAATTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACAACAATTG                                                         >  SRR19973067.1848978/1‑150 (MQ=60)
                                                                                                TACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGT                                                       <  SRR19973067.2233023/150‑1 (MQ=60)
                                                                                                 ACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTG                                                      <  SRR19973067.3456615/150‑1 (MQ=60)
                                                                                                 ACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTG                                                      <  SRR19973067.949044/150‑1 (MQ=60)
                                                                                                    TTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTAC                                                   <  SRR19973067.2976145/150‑1 (MQ=60)
                                                                                                     TGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACT                                                  <  SRR19973067.2805179/150‑1 (MQ=60)
                                                                                                        CTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGT                                               >  SRR19973067.11422/1‑150 (MQ=60)
                                                                                                        CTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGT                                               <  SRR19973067.2637064/150‑1 (MQ=60)
                                                                                                            ATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACC                                           >  SRR19973067.1108198/1‑150 (MQ=60)
                                                                                                            ATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACC                                           >  SRR19973067.981581/1‑150 (MQ=60)
                                                                                                            ATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACC                                           >  SRR19973067.3084772/1‑150 (MQ=60)
                                                                                                                GCTTTCAGTTTTGATACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACA                                       >  SRR19973067.2844047/1‑150 (MQ=60)
                                                                                                                      AGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCAT                                 >  SRR19973067.1428835/1‑150 (MQ=60)
                                                                                                                          TTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGT                             <  SRR19973067.772465/150‑1 (MQ=60)
                                                                                                                            GAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGT                           <  SRR19973067.1930288/150‑1 (MQ=60)
                                                                                                                             ATACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAATTTACACTGGACAAATCGTACCACAATTGGTGTACTGGTAACCGACACAGAATGTGTGTC                          >  SRR19973067.2469236/1‑150 (MQ=60)
                                                                                                                             AGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTC                          >  SRR19973067.48480/1‑150 (MQ=60)
                                                                                                                             AGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTANCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTC                          >  SRR19973067.1812954/1‑150 (MQ=60)
                                                                                                                              GACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCT                         >  SRR19973067.1134869/1‑150 (MQ=60)
                                                                                                                                CAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAT                       <  SRR19973067.1957191/150‑1 (MQ=60)
                                                                                                                                   ATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTT                    >  SRR19973067.1336150/1‑150 (MQ=60)
                                                                                                                                    TGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTT                   >  SRR19973067.3367316/1‑150 (MQ=60)
                                                                                                                                    TGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTT                   >  SRR19973067.3367311/1‑150 (MQ=60)
                                                                                                                                      AAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCA                 <  SRR19973067.2323255/150‑1 (MQ=60)
                                                                                                                                       AGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCAT                <  SRR19973067.1262402/150‑1 (MQ=60)
                                                                                                                                        GTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATG               <  SRR19973067.702632/150‑1 (MQ=60)
                                                                                                                                            TAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAA           >  SRR19973067.3121567/1‑150 (MQ=60)
                                                                                                                                            TAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATGTGTGTCTATTTTTCATGTAAA           >  SRR19973067.1200719/1‑150 (MQ=60)
                                                                                                                                             AGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAG          >  SRR19973067.2695277/1‑150 (MQ=60)
                                                                                                                                              GTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGG         >  SRR19973067.2919872/1‑150 (MQ=60)
                                                                                                                                                  CTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTGACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAAT     >  SRR19973067.739302/1‑150 (MQ=60)
                                                                                                                                                  CTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAAT     >  SRR19973067.739411/1‑150 (MQ=60)
                                                                                                                                                   TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATT    >  SRR19973067.410168/1‑150 (MQ=60)
                                                                                                                                                     TTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTT  <  SRR19973067.632524/150‑1 (MQ=60)
                                                                                                                                                     TTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTT  <  SRR19973067.631957/150‑1 (MQ=60)
                                                                                                                                                     TTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTCTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTT  <  SRR19973067.1832357/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTT  >  CP009273/652448‑652746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑