Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 652,597 C→T 100% intergenic (+24/‑151) pagP → / → cspE phospholipid:lipid A palmitoyltransferase/constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273652,5970CT97.8% 770.2 / 4.0 231intergenic (+24/‑151)pagP/cspEphospholipid:lipid A palmitoyltransferase/constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (3/2);  new base T (118/107);  total (122/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 235 total aligned reads displayed.

CTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAAT  >  CP009273/652451‑652743
                                                                                                                                                  |                                                                                                                                                  
ctcCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACtttttt                                                                                                                                                 <  2:3495400/150‑1 (MQ=255)
 tcCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTa                                                                                                                                                >  2:1077319/1‑150 (MQ=255)
           aCTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAg                                                                                                                                      <  2:33445/150‑1 (MQ=255)
           aCTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAg                                                                                                                                      <  1:308345/150‑1 (MQ=255)
           aCTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAg                                                                                                                                      >  2:4503192/1‑150 (MQ=255)
           aCTGCCAGTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGCTGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAg                                                                                                                                      <  2:4811957/150‑1 (MQ=255)
                cATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTaaa                                                                                                                                 >  1:110153/1‑150 (MQ=255)
                   tGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATa                                                                                                                              <  1:1249508/150‑1 (MQ=255)
                   tGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATa                                                                                                                              <  1:2719785/150‑1 (MQ=255)
                   tGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATa                                                                                                                              <  2:4125576/150‑1 (MQ=255)
                      ccTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGt                                                                                                                           >  2:1170072/1‑150 (MQ=255)
                      ccTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGt                                                                                                                           <  1:909907/150‑1 (MQ=255)
                       cTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTa                                                                                                                          >  2:619741/1‑150 (MQ=255)
                         ccGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAAc                                                                                                                        >  1:1498519/1‑150 (MQ=255)
                           gTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAATTCAATAGCTAAAATAAGTAACAt                                                                                                                      >  2:4473925/1‑150 (MQ=255)
                           gTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACAt                                                                                                                      <  1:3196971/150‑1 (MQ=255)
                            tGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATc                                                                                                                     <  1:3616604/150‑1 (MQ=255)
                            tGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATc                                                                                                                     >  1:707851/1‑150 (MQ=255)
                               gTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCaaa                                                                                                                  >  1:18812/1‑150 (MQ=255)
                                ttATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCaaaa                                                                                                                 <  2:3709587/150‑1 (MQ=255)
                                  aTGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAAt                                                                                                               <  2:1995138/150‑1 (MQ=255)
                                   tGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATa                                                                                                              >  2:1435832/1‑150 (MQ=255)
                                   tGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATa                                                                                                              >  1:3816189/1‑150 (MQ=255)
                                     gCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAAc                                                                                                            <  2:2481428/150‑1 (MQ=255)
                                      cccAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAAcg                                                                                                           <  1:3486796/150‑1 (MQ=255)
                                       ccAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAAcgc                                                                                                          >  2:3889881/1‑150 (MQ=255)
                                           tGACTTTTCTGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGATAAAATAAGTAACATCAAAAATAACGCGACt                                                                                                      <  1:200957/150‑1 (MQ=255)
                                             aCTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACttt                                                                                                    <  2:4593079/150‑1 (MQ=255)
                                             aCTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACtta                                                                                                    <  2:2802013/150‑2 (MQ=255)
                                                tttCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCTGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTAt                                                                                                 <  2:1447423/150‑1 (MQ=255)
                                                tttCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTAt                                                                                                 <  2:158672/150‑1 (MQ=255)
                                                tttCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTAt                                                                                                 <  2:2177752/150‑1 (MQ=255)
                                                  tCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCa                                                                                               <  1:4900008/150‑1 (MQ=255)
                                                  tCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCa                                                                                               <  1:383474/150‑1 (MQ=255)
                                                   cAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCAc                                                                                              <  1:1742544/150‑1 (MQ=255)
                                                    aGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACt                                                                                             <  1:4659108/150‑1 (MQ=255)
                                                      aTGACCTACATTCAGGGTACCTACAACAATTGCAATGTGTACTTTGCCTGGATGCGCTTTCATTTTTGAGACAATTGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACttt                                                                                           <  1:2497372/150‑1 (MQ=255)
                                                       tGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACtttt                                                                                          <  1:1590858/150‑1 (MQ=255)
                                                         aCCTACATTCCGTGTACCTAAAACAATGGCAATGTGTTCTTTGCCTTGATGAGCTTTCAGTTTTGATACAAATGATTTTTTATTAACTTTTTTATAATCAATAGCTAAAATAATTATCATCAAAAATAACGCGACTTTTATCACTTTTTa                                                                                        <  1:4350283/150‑1 (MQ=255)
                                                            tACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTa                                                                                     <  2:633982/150‑1 (MQ=255)
                                                            tACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTa                                                                                     <  2:4975884/150‑1 (MQ=255)
                                                             aCATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTaa                                                                                    <  1:346147/150‑1 (MQ=255)
                                                              cATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTaaa                                                                                   >  2:2478684/1‑150 (MQ=255)
                                                                   cGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTac                                                                              >  2:4698648/1‑150 (MQ=255)
                                                                     ggTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTacac                                                                            <  2:900480/150‑1 (MQ=255)
                                                                     ggTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTacac                                                                            >  1:5646/1‑150 (MQ=255)
                                                                       tACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTg                                                                          <  1:1258807/150‑1 (MQ=255)
                                                                        aCCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTgg                                                                         <  1:1131529/150‑1 (MQ=255)
                                                                         ccTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGa                                                                        >  1:598524/1‑150 (MQ=255)
                                                                         ccTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGa                                                                        >  1:3135901/1‑150 (MQ=255)
                                                                          cTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGAc                                                                       >  2:5025938/1‑150 (MQ=255)
                                                                           tACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACa                                                                      >  1:2644017/1‑150 (MQ=255)
                                                                               acaaTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGc                                                                  <  1:1401577/150‑1 (MQ=255)
                                                                                caaTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCg                                                                 >  2:3193832/1‑150 (MQ=255)
                                                                                caaTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCg                                                                 >  2:3227398/1‑150 (MQ=255)
                                                                                caaTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCg                                                                 >  2:2967740/1‑150 (MQ=255)
                                                                                 aaTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGt                                                                >  2:1287053/1‑150 (MQ=255)
                                                                                  aTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTa                                                               <  2:2158309/150‑1 (MQ=255)
                                                                                   tGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTAc                                                              >  1:60583/1‑150 (MQ=255)
                                                                                    ggCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTAcc                                                             >  1:2827989/1‑150 (MQ=255)
                                                                                     gCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACca                                                            >  1:1826203/1‑150 (MQ=255)
                                                                                       aaTGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACcaca                                                          <  2:748047/150‑1 (MQ=255)
                                                                                          gtgtACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAAtt                                                       >  1:4800747/1‑150 (MQ=255)
                                                                                             tACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGgt                                                    >  1:815352/1‑150 (MQ=255)
                                                                                               cTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGgtgt                                                  <  2:592411/150‑1 (MQ=255)
                                                                                                 ttGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTAc                                                >  2:4261665/1‑150 (MQ=255)
                                                                                                   gCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTg                                              >  2:4552741/1‑150 (MQ=255)
                                                                                                   gCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTg                                              >  2:4183318/1‑150 (MQ=255)
                                                                                                     cTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGt                                            >  1:4435405/1‑150 (MQ=255)
                                                                                                      tGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTa                                           >  2:1909164/1‑150 (MQ=255)
                                                                                                         aTGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAAcc                                        <  2:2309820/150‑1 (MQ=255)
                                                                                                          tGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCg                                       >  1:1118680/1‑150 (MQ=255)
                                                                                                            cgcTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGac                                     >  1:4970809/1‑150 (MQ=255)
                                                                                                            cgcTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGac                                     >  1:2023999/1‑150 (MQ=255)
                                                                                                            cgcTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGac                                     <  1:3272615/150‑1 (MQ=255)
                                                                                                             gcTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGaca                                    <  2:2171562/150‑1 (MQ=255)
                                                                                                              cTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTCTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGacac                                   >  2:4553453/1‑150 (MQ=255)
                                                                                                                  cAGTTTTGAGACAAATGAAGTTTGAGTAACTTCTTTAAAATCAATAGCTAAAATAAGGAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCa                               <  2:4360985/150‑1 (MQ=255)
                                                                                                                   aGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCAt                              >  1:2918863/1‑150 (MQ=255)
                                                                                                                   aGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCAt                              >  2:2228333/1‑150 (MQ=255)
                                                                                                                     ttttGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCAttt                            <  2:2935945/150‑1 (MQ=255)
                                                                                                                         gagaCAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTgtgt                        >  1:280393/1‑150 (MQ=255)
                                                                                                                          agaCAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTc                       <  2:2715923/150‑1 (MQ=255)
                                                                                                                             cAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAt                    >  2:4012878/1‑150 (MQ=255)
                                                                                                                              aaaTGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAtt                   <  1:4529455/150‑1 (MQ=255)
                                                                                                                              aaaTGAAGTTTTAGTAACTTTTTTAAAATAAATAGCTAAAATAAGTTACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAtt                   <  2:5146747/150‑1 (MQ=255)
                                                                                                                               aaTGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAttt                  <  1:2092062/150‑1 (MQ=255)
                                                                                                                                aTGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAtttt                 >  2:4894969/1‑150 (MQ=255)
                                                                                                                                aTGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAtttt                 >  1:4502159/1‑150 (MQ=255)
                                                                                                                                aTGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACAACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAtttt                 >  1:1230395/1‑150 (MQ=255)
                                                                                                                                 tGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAttttt                <  1:4087657/150‑1 (MQ=255)
                                                                                                                                  gAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTc               >  1:4022146/1‑150 (MQ=255)
                                                                                                                                   aaGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCa              >  2:2553315/1‑150 (MQ=255)
                                                                                                                                       tttAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTa          <  1:256347/150‑1 (MQ=255)
                                                                                                                                       tttAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACACGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTa          >  1:3854389/1‑150 (MQ=255)
                                                                                                                                          aGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCAGTTGTGTCTATTTTTCATGTAAAg       >  2:2952352/1‑150 (MQ=255)
                                                                                                                                           gTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAgg      >  1:4943306/1‑150 (MQ=255)
                                                                                                                                           gTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAgg      <  2:196397/150‑1 (MQ=255)
                                                                                                                                              aCTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATTTAAAGGTaa   >  2:3468823/1‑150 (MQ=255)
                                                                                                                                               ctttttTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATGTGTGTCTATTTTTCATGTAAAGGTAAt  >  2:2739596/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAAT  >  CP009273/652451‑652743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGG  >  CP009273/652448‑652739
                                                                                                                                                     |                                                                                                                                              
CCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAACTTT                                                                                                                                                >  SRR19973063.3657253/1‑150 (MQ=60)
   CTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTT                                                                                                                                             <  SRR19973063.2514965/150‑1 (MQ=60)
     CCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAA                                                                                                                                           >  SRR19973063.2724698/1‑150 (MQ=60)
         GTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATC                                                                                                                                       >  SRR19973063.1458667/1‑150 (MQ=60)
          TTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCA                                                                                                                                      <  SRR19973063.1302973/150‑1 (MQ=60)
            CTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAAT                                                                                                                                    <  SRR19973063.3760589/150‑1 (MQ=60)
             TACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTT‑TTTAAAATTAAAAG                                                                                                                                  >  SRR19973063.3868303/1‑150 (MQ=60)
              ACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAG                                                                                                                                  <  SRR19973063.309249/150‑1 (MQ=60)
                 GCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTA                                                                                                                               >  SRR19973063.4848233/1‑150 (MQ=60)
                  CCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAA                                                                                                                              >  SRR19973063.2945311/1‑150 (MQ=60)
                   CATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAA                                                                                                                             >  SRR19973063.110520/1‑150 (MQ=60)
                   CATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAA                                                                                                                             >  SRR19973063.1250932/1‑150 (MQ=60)
                      TGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATA                                                                                                                          <  SRR19973063.2728569/150‑1 (MQ=60)
                      TGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATA                                                                                                                          <  SRR19973063.1253235/150‑1 (MQ=60)
                      TGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATA                                                                                                                          <  SRR19973063.1253234/150‑1 (MQ=60)
                         CCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGT                                                                                                                       <  SRR19973063.912580/150‑1 (MQ=60)
                            CCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAAC                                                                                                                    >  SRR19973063.1503108/1‑150 (MQ=60)
                              GTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACAT                                                                                                                  <  SRR19973063.3207249/150‑1 (MQ=60)
                               TGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATC                                                                                                                 >  SRR19973063.3304403/1‑150 (MQ=60)
                               TGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATC                                                                                                                 >  SRR19973063.709932/1‑150 (MQ=60)
                               TGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATC                                                                                                                 >  SRR19973063.709869/1‑150 (MQ=60)
                               TGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATC                                                                                                                 <  SRR19973063.3628213/150‑1 (MQ=60)
                                  GTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAA                                                                                                              >  SRR19973063.18881/1‑150 (MQ=60)
                                  GTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAA                                                                                                              >  SRR19973063.18883/1‑150 (MQ=60)
                                    TATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAA                                                                                                            >  SRR19973063.3198582/1‑150 (MQ=60)
                                      TGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATA                                                                                                          >  SRR19973063.3828338/1‑150 (MQ=60)
                                       GGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAA                                                                                                         >  SRR19973063.4277005/1‑150 (MQ=60)
                                       GGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAA                                                                                                         <  SRR19973063.3598659/150‑1 (MQ=60)
                                        GCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAAC                                                                                                        <  SRR19973063.4567791/150‑1 (MQ=60)
                                         CCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACG                                                                                                       <  SRR19973063.3497940/150‑1 (MQ=60)
                                              TGACTTTTCTGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGATAAAATAAGTAACATCAAAAATAACGCGACT                                                                                                  <  SRR19973063.201567/150‑1 (MQ=60)
                                                    TTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATC                                                                                            <  SRR19973063.1173598/150‑1 (MQ=60)
                                                     TCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCA                                                                                           <  SRR19973063.384536/150‑1 (MQ=60)
                                                     TCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCA                                                                                           <  SRR19973063.384567/150‑1 (MQ=60)
                                                     TCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCA                                                                                           <  SRR19973063.4915828/150‑1 (MQ=60)
                                                     TCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCA                                                                                           <  SRR19973063.4546364/150‑1 (MQ=60)
                                                      CAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCAC                                                                                          <  SRR19973063.1747840/150‑1 (MQ=60)
                                                       AGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACT                                                                                         <  SRR19973063.4673975/150‑1 (MQ=60)
                                                       AGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACT                                                                                         >  SRR19973063.4961937/1‑150 (MQ=60)
                                                         ATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTT                                                                                       <  SRR19973063.2010058/150‑1 (MQ=60)
                                                         ATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTT                                                                                       <  SRR19973063.2009903/150‑1 (MQ=60)
                                                         ATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTT                                                                                       <  SRR19973063.1214310/150‑1 (MQ=60)
                                                         ATGACCTACATTCAGGGTACCTACAACAATTGCAATGTGTACTTTGCCTGGATGCGCTTTCATTTTTGAGACAATTGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTT                                                                                       <  SRR19973063.2505339/150‑1 (MQ=60)
                                                          TGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTT                                                                                      >  SRR19973063.4990577/1‑150 (MQ=60)
                                                          TGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTT                                                                                      <  SRR19973063.1595736/150‑1 (MQ=60)
                                                            ACCTACATTCCGTGTACCTAAAACAATGGCAATGTGTTCTTTGCCTTGATGAGCTTTCAGTTTTGATACAAATGATTTTTTATTAACTTTTTTATAATCAATAGCTAAAATAATTATCATCAAAAATAACGCGACTTTTATCACTTTTTA                                                                                    <  SRR19973063.4364181/150‑1 (MQ=60)
                                                            ACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTA                                                                                    >  SRR19973063.2831880/1‑150 (MQ=60)
                                                            ACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTA                                                                                    <  SRR19973063.3110727/150‑1 (MQ=60)
                                                                ACATTCCGGGTACCTACAACAATGTCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAA                                                                                <  SRR19973063.347329/150‑1 (MQ=60)
                                                                ACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAA                                                                                <  SRR19973063.347145/150‑1 (MQ=60)
                                                                  ATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAG                                                                              >  SRR19973063.3960496/1‑150 (MQ=60)
                                                                    TCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTT                                                                            >  SRR19973063.2019059/1‑150 (MQ=60)
                                                                        GGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACAC                                                                        >  SRR19973063.1123284/1‑150 (MQ=60)
                                                                        GGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACAC                                                                        >  SRR19973063.5674/1‑150 (MQ=60)
                                                                          TACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTG                                                                      <  SRR19973063.1262550/150‑1 (MQ=60)
                                                                          TACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTG                                                                      >  SRR19973063.2059023/1‑150 (MQ=60)
                                                                           ACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGG                                                                     <  SRR19973063.1134963/150‑1 (MQ=60)
                                                                            CCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGA                                                                    >  SRR19973063.600286/1‑150 (MQ=60)
                                                                            CCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGA                                                                    >  SRR19973063.600289/1‑150 (MQ=60)
                                                                            CCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGA                                                                    >  SRR19973063.3145987/1‑150 (MQ=60)
                                                                              TACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACA                                                                  >  SRR19973063.2652469/1‑150 (MQ=60)
                                                                                  ACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGC                                                              <  SRR19973063.1405817/150‑1 (MQ=60)
                                                                                  ACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGC                                                              <  SRR19973063.5178066/150‑1 (MQ=60)
                                                                                  ACAATGGCAATGTGTACTGTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGC                                                              <  SRR19973063.4416081/150‑1 (MQ=60)
                                                                                    AATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGT                                                            >  SRR19973063.879398/1‑150 (MQ=60)
                                                                                    AATGGCAAAGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAAAAGATAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTATTTAGTAAATTTACACTGGACAAAGCGT                                                            >  SRR19973063.731538/1‑150 (MQ=60)
                                                                                     ATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTA                                                           >  SRR19973063.4827223/1‑150 (MQ=60)
                                                                                     ATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGAGTA                                                           >  SRR19973063.584126/1‑150 (MQ=60)
                                                                                      TGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTAC                                                          >  SRR19973063.60793/1‑150 (MQ=60)
                                                                                       GGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACC                                                         >  SRR19973063.2837076/1‑150 (MQ=60)
                                                                                       GGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACC                                                         >  SRR19973063.2837074/1‑150 (MQ=60)
                                                                                        GCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCA                                                        >  SRR19973063.1831880/1‑150 (MQ=60)
                                                                                           ATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAA                                                     >  SRR19973063.958694/1‑150 (MQ=60)
                                                                                            TGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAAT                                                    <  SRR19973063.4395319/150‑1 (MQ=60)
                                                                                             GTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATT                                                   >  SRR19973063.4816089/1‑150 (MQ=60)
                                                                                               GTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGG                                                 >  SRR19973063.110771/1‑150 (MQ=60)
                                                                                                TACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGT                                                >  SRR19973063.817708/1‑150 (MQ=60)
                                                                                                 ACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTG                                               <  SRR19973063.491604/150‑1 (MQ=60)
                                                                                                 ACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTG                                               >  SRR19973063.1271308/1‑150 (MQ=60)
                                                                                                       CCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGG                                         <  SRR19973063.1509611/150‑1 (MQ=60)
                                                                                                       CCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGG                                         >  SRR19973063.4488148/1‑150 (MQ=60)
                                                                                                        CTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGT                                        >  SRR19973063.4449639/1‑150 (MQ=60)
                                                                                                          GGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAA                                      >  SRR19973063.245677/1‑150 (MQ=60)
                                                                                                           GATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAAC                                     <  SRR19973063.3858308/150‑1 (MQ=60)
                                                                                                           GATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAAC                                     >  SRR19973063.261217/1‑150 (MQ=60)
                                                                                                             TGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCG                                   >  SRR19973063.1122077/1‑150 (MQ=60)
                                                                                                             TGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCG                                   <  SRR19973063.621561/150‑1 (MQ=60)
                                                                                                               CGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGAC                                 >  SRR19973063.1775156/1‑150 (MQ=60)
                                                                                                               CGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGAC                                 >  SRR19973063.2030318/1‑150 (MQ=60)
                                                                                                               CGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGAC                                 <  SRR19973063.3283105/150‑1 (MQ=60)
                                                                                                               CGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGAC                                 <  SRR19973063.3283120/150‑1 (MQ=60)
                                                                                                               CGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGAC                                 >  SRR19973063.4986874/1‑150 (MQ=60)
                                                                                                                      AGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCAT                          >  SRR19973063.2928169/1‑150 (MQ=60)
                                                                                                                         TTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTG                       >  SRR19973063.2126029/1‑150 (MQ=60)
                                                                                                                          TTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGT                      >  SRR19973063.3119368/1‑150 (MQ=60)
                                                                                                                            GAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGT                    >  SRR19973063.281225/1‑150 (MQ=60)
                                                                                                                              GACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCT                  <  SRR19973063.2667363/150‑1 (MQ=60)
                                                                                                                                CAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAT                >  SRR19973063.4052364/1‑150 (MQ=60)
                                                                                                                                CAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAT                <  SRR19973063.2043966/150‑1 (MQ=60)
                                                                                                                                 AAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATT               <  SRR19973063.4543983/150‑1 (MQ=60)
                                                                                                                                  AATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTT              <  SRR19973063.2098596/150‑1 (MQ=60)
                                                                                                                                  AATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATGTGTGTCTATTT              >  SRR19973063.3372759/1‑150 (MQ=60)
                                                                                                                                   ATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTT             >  SRR19973063.2739843/1‑150 (MQ=60)
                                                                                                                                   ATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTT             >  SRR19973063.4688410/1‑150 (MQ=60)
                                                                                                                                   ATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTT             >  SRR19973063.4516612/1‑150 (MQ=60)
                                                                                                                                   ATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTT             <  SRR19973063.1605799/150‑1 (MQ=60)
                                                                                                                                   ATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACAACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTT             >  SRR19973063.1234070/1‑150 (MQ=60)
                                                                                                                                    TGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTT            <  SRR19973063.4100753/150‑1 (MQ=60)
                                                                                                                                     GAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTC           >  SRR19973063.4035048/1‑150 (MQ=60)
                                                                                                                                         TTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGT       >  SRR19973063.3663846/1‑150 (MQ=60)
                                                                                                                                          TTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTA      <  SRR19973063.453454/150‑1 (MQ=60)
                                                                                                                                          TTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTA      <  SRR19973063.257116/150‑1 (MQ=60)
                                                                                                                                          TTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTA      <  SRR19973063.2859524/150‑1 (MQ=60)
                                                                                                                                          TTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACACGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTA      >  SRR19973063.3866644/1‑150 (MQ=60)
                                                                                                                                           TTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAA     >  SRR19973063.1714979/1‑150 (MQ=60)
                                                                                                                                              GTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGG  >  SRR19973063.4959295/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                              
CCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGG  >  CP009273/652448‑652739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑