Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 652,597 C→T 100% intergenic (+24/‑151) pagP → / → cspE phospholipid:lipid A palmitoyltransferase/constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273652,5970CT98.2% 548.1 / 0.5 167intergenic (+24/‑151)pagP/cspEphospholipid:lipid A palmitoyltransferase/constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (79/85);  total (80/87)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 169 total aligned reads displayed.

TCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTA  >  CP009273/652452‑652741
                                                                                                                                                 |                                                                                                                                                
tcCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAActtttt                                                                                                                                                <  1:1905414/148‑1 (MQ=255)
   cGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTaa                                                                                                                                             <  2:1271169/148‑1 (MQ=255)
    tgTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATTCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTaaa                                                                                                                                            <  1:393147/147‑1 (MQ=255)
    ggTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTaaa                                                                                                                                            <  1:3015438/148‑1 (MQ=255)
     gTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAACTTTTTTaaaa                                                                                                                                           <  2:1522511/148‑1 (MQ=255)
      ttCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAAt                                                                                                                                          <  2:324899/148‑1 (MQ=255)
      ttCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAAt                                                                                                                                          <  1:529055/148‑1 (MQ=255)
      ttCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAAt                                                                                                                                          >  1:2157100/1‑148 (MQ=255)
      ttCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAAt                                                                                                                                          >  1:2156973/1‑148 (MQ=255)
         tACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCaa                                                                                                                                       <  1:2018392/148‑1 (MQ=255)
         tACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCaa                                                                                                                                       <  1:1424291/148‑1 (MQ=255)
          aCTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAAt                                                                                                                                      <  2:1544772/148‑1 (MQ=255)
           cTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATa                                                                                                                                     <  1:472635/148‑1 (MQ=255)
           cTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATa                                                                                                                                     >  1:3338728/1‑148 (MQ=255)
           cTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATa                                                                                                                                     >  1:2861197/1‑148 (MQ=255)
                 ttGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTaaa                                                                                                                               <  2:3350419/148‑1 (MQ=255)
                  tGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTaaaa                                                                                                                              >  2:885360/1‑148 (MQ=255)
                    gCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATa                                                                                                                            >  2:3152009/1‑148 (MQ=255)
                    gCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATa                                                                                                                            >  2:3152469/1‑148 (MQ=255)
                     ccTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATaa                                                                                                                           <  1:992285/148‑1 (MQ=255)
                     ccTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATaa                                                                                                                           <  2:580343/148‑1 (MQ=255)
                       tCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGt                                                                                                                         <  2:1402816/148‑1 (MQ=255)
                        ccGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTa                                                                                                                        <  1:3499554/148‑1 (MQ=255)
                         cGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTaa                                                                                                                       <  2:269982/148‑1 (MQ=255)
                         cGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTaa                                                                                                                       <  2:269985/148‑1 (MQ=255)
                               ttATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCaa                                                                                                                 >  2:3218423/1‑148 (MQ=255)
                                   ggCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAAt                                                                                                             <  1:2425153/148‑1 (MQ=255)
                                    gCCCAGTGACTTTTCAGATGACCTACAGTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATa                                                                                                            <  2:2412081/148‑1 (MQ=255)
                                       cAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACATTGGCTATGTGTACTTTGCCTGGATGCGCTTTGAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAATTAAGTATCATCAAAAATAAcg                                                                                                         <  2:401091/148‑1 (MQ=255)
                                       cAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTCAAATAAGTAACATCAAAAATAAcg                                                                                                         >  1:2792812/1‑148 (MQ=255)
                                       cAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAAcg                                                                                                         <  1:2855383/148‑1 (MQ=255)
                                        aGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAAcgc                                                                                                        >  1:1475244/1‑148 (MQ=255)
                                        aGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAAcgc                                                                                                        >  1:3220474/1‑148 (MQ=255)
                                         gTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAAcgcg                                                                                                       <  1:1327983/148‑1 (MQ=255)
                                             cTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACtt                                                                                                   >  1:1335116/1‑148 (MQ=255)
                                             cTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACtt                                                                                                   >  1:581641/1‑148 (MQ=255)
                                                ttCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTTAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTa                                                                                                <  1:3340000/148‑1 (MQ=255)
                                                ttCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTa                                                                                                <  2:703391/148‑1 (MQ=255)
                                                ttCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTa                                                                                                <  2:703218/148‑1 (MQ=255)
                                                  cAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATc                                                                                              <  1:3169848/148‑1 (MQ=255)
                                                     aTGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACt                                                                                           <  1:1086104/148‑1 (MQ=255)
                                                     aTGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACt                                                                                           <  1:2499036/148‑1 (MQ=255)
                                                     aTGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACt                                                                                           <  1:1086108/148‑1 (MQ=255)
                                                          cTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTa                                                                                      <  2:3073461/148‑1 (MQ=255)
                                                           tACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAg                                                                                     >  2:2215637/1‑148 (MQ=255)
                                                           tACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAg                                                                                     <  2:337471/148‑1 (MQ=255)
                                                            aCATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGt                                                                                    >  1:236509/1‑148 (MQ=255)
                                                              aTTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTaa                                                                                  <  1:974030/148‑1 (MQ=255)
                                                               ttCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTaaa                                                                                 <  2:1848739/148‑1 (MQ=255)
                                                                    ggTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTac                                                                            >  2:1986713/1‑148 (MQ=255)
                                                                       aCCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACt                                                                         >  2:1131050/1‑148 (MQ=255)
                                                                        ccTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTg                                                                        >  1:740711/1‑148 (MQ=255)
                                                                         cTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTgg                                                                       >  1:3372777/1‑148 (MQ=255)
                                                                         cTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTgg                                                                       >  2:2114523/1‑148 (MQ=255)
                                                                         cTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTgg                                                                       >  1:1718456/1‑148 (MQ=255)
                                                                          tACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGa                                                                      >  2:1334276/1‑148 (MQ=255)
                                                                           acaacaATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGAc                                                                     >  1:748327/1‑148 (MQ=255)
                                                                             aacaaTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACaa                                                                   <  2:3182460/148‑1 (MQ=255)
                                                                             aacaaTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACaa                                                                   <  2:1810814/148‑1 (MQ=255)
                                                                              acaaTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACaaa                                                                  <  2:3356072/148‑1 (MQ=255)
                                                                               caaTTGAAATGTGTACTTTGCCTGGATGCTCTTTCAGTTTTGAGACAAATTAAGTTTTAGTAAATTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATAACTTTTTAGTAAAGTTACACTGGACAAAg                                                                 <  1:2957233/148‑1 (MQ=255)
                                                                               caaTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAg                                                                 >  2:3297783/1‑148 (MQ=255)
                                                                                  tGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGt                                                              <  1:753105/148‑1 (MQ=255)
                                                                                   ggCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTa                                                             <  2:2430909/148‑1 (MQ=255)
                                                                                   ggCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTa                                                             <  2:669605/148‑1 (MQ=255)
                                                                                   ggCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTa                                                             >  1:751436/1‑148 (MQ=255)
                                                                                    gCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAATGCGACTTTTATCACTTTTTATTAAAGTTACACTGGACAAAGCGTAc                                                            >  1:3094246/1‑148 (MQ=255)
                                                                                    gCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTAc                                                            >  2:808127/1‑148 (MQ=255)
                                                                                        tgtgTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACcaca                                                        >  2:2513845/1‑148 (MQ=255)
                                                                                         gtgtACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACaa                                                       >  2:2248511/1‑148 (MQ=255)
                                                                                             aCTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTgg                                                   <  2:2874658/148‑1 (MQ=255)
                                                                                             aCTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTgg                                                   <  1:2340293/148‑1 (MQ=255)
                                                                                                ttGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGgtgt                                                >  1:3480508/1‑148 (MQ=255)
                                                                                                ttGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGgtgt                                                >  1:481759/1‑148 (MQ=255)
                                                                                                ttGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGgtgt                                                >  1:2703316/1‑148 (MQ=255)
                                                                                                        aTGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTaa                                        >  1:3453843/1‑148 (MQ=255)
                                                                                                           cgcTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCg                                     >  1:168148/1‑148 (MQ=255)
                                                                                                            gcTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGa                                    <  1:2641683/148‑1 (MQ=255)
                                                                                                              tttCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGaca                                  <  2:514169/148‑1 (MQ=255)
                                                                                                                tCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGAcaca                                <  2:236509/148‑1 (MQ=255)
                                                                                                                  aGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGc                              <  2:2812550/148‑1 (MQ=255)
                                                                                                                    ttttGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCAt                            >  1:2728646/1‑148 (MQ=255)
                                                                                                                      ttGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCAttt                          <  2:887783/148‑1 (MQ=255)
                                                                                                                       tGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTtg                         <  1:875134/148‑1 (MQ=255)
                                                                                                                           aCAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTGGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTc                     <  1:3285685/148‑1 (MQ=255)
                                                                                                                           aCAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTc                     >  2:1409885/1‑148 (MQ=255)
                                                                                                                            cAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCt                    >  2:100755/1‑148 (MQ=255)
                                                                                                                               aTGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAtt                 >  1:2084803/1‑148 (MQ=255)
                                                                                                                                tGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCGCTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAttt                <  2:914453/148‑1 (MQ=255)
                                                                                                                                tGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAttt                <  2:912401/148‑1 (MQ=255)
                                                                                                                                 gAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAtttt               <  1:3138112/148‑1 (MQ=255)
                                                                                                                                  aaGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAttttt              <  1:2555566/148‑1 (MQ=255)
                                                                                                                                   aGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTc             <  1:1986713/148‑1 (MQ=255)
                                                                                                                                      tttAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATg          >  1:2429289/1‑148 (MQ=255)
                                                                                                                                       ttAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGt         <  1:2513845/148‑1 (MQ=255)
                                                                                                                                         aGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTaa       <  2:1027709/148‑1 (MQ=255)
                                                                                                                                           tAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACCCAGCATGTGTGTCTATTTTTCATGTAAAg     >  2:2901479/1‑148 (MQ=255)
                                                                                                                                            aaCTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAgg    >  2:2576274/1‑148 (MQ=255)
                                                                                                                                            aaCTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACAGACACAGCATTTGTGTCTATTTTTCATGTAAAgg    >  2:1114627/1‑148 (MQ=255)
                                                                                                                                             aCTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGt   >  2:1221667/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTA  >  CP009273/652452‑652741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTT  >  CP009273/652448‑652745
                                                                                                                                                     |                                                                                                                                                    
CCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTT                                                                                                                                                      >  SRR19973065.3036242/1‑150 (MQ=60)
    TCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTA                                                                                                                                                  <  SRR19973065.1917931/150‑1 (MQ=60)
        TGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATTCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAAT                                                                                                                                              <  SRR19973065.395891/150‑1 (MQ=60)
        GGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAAT                                                                                                                                              >  SRR19973065.2170842/1‑150 (MQ=60)
        GGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAAT                                                                                                                                              >  SRR19973065.2170969/1‑150 (MQ=60)
        GGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAAT                                                                                                                                              >  SRR19973065.2527213/1‑150 (MQ=60)
        GGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAAT                                                                                                                                              <  SRR19973065.3033905/150‑1 (MQ=60)
        GGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAAT                                                                                                                                              >  SRR19973065.392482/1‑150 (MQ=60)
          TTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCA                                                                                                                                            <  SRR19973065.532521/150‑1 (MQ=60)
             TACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATA                                                                                                                                         <  SRR19973065.1433574/150‑1 (MQ=60)
             TACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATA                                                                                                                                         >  SRR19973065.3359118/1‑150 (MQ=60)
             TACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATA                                                                                                                                         >  SRR19973065.2878765/1‑150 (MQ=60)
             TACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATA                                                                                                                                         <  SRR19973065.2031387/150‑1 (MQ=60)
              ACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAG                                                                                                                                        >  SRR19973065.2350030/1‑150 (MQ=60)
               CTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGC                                                                                                                                       <  SRR19973065.475867/150‑1 (MQ=60)
                TGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCT                                                                                                                                      >  SRR19973065.3489804/1‑150 (MQ=60)
                    ATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAA                                                                                                                                  >  SRR19973065.3427592/1‑150 (MQ=60)
                    ATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAA                                                                                                                                  >  SRR19973065.1680835/1‑150 (MQ=60)
                    ATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAA                                                                                                                                  >  SRR19973065.2355050/1‑150 (MQ=60)
                         CCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGT                                                                                                                             <  SRR19973065.998763/150‑1 (MQ=60)
                         CCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGT                                                                                                                             <  SRR19973065.998878/150‑1 (MQ=60)
                            CCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAAC                                                                                                                          <  SRR19973065.3520813/150‑1 (MQ=60)
                                GGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCA                                                                                                                      <  SRR19973065.316757/150‑1 (MQ=60)
                                       GGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAA                                                                                                               <  SRR19973065.2440454/150‑1 (MQ=60)
                                         CCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTCAAATAAGTAACATCAAAAATAACG                                                                                                             >  SRR19973065.2810123/1‑150 (MQ=60)
                                          CCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGC                                                                                                            >  SRR19973065.1484680/1‑150 (MQ=60)
                                          CCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGC                                                                                                            >  SRR19973065.3239830/1‑150 (MQ=60)
                                           CAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCG                                                                                                           <  SRR19973065.2872931/150‑1 (MQ=60)
                                           CAGTGACTTTTCAGATGACCTACAGTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCG                                                                                                           <  SRR19973065.1282310/150‑1 (MQ=60)
                                             GTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGAC                                                                                                         <  SRR19973065.1336765/150‑1 (MQ=60)
                                               GACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTT                                                                                                       >  SRR19973065.1343959/1‑150 (MQ=60)
                                               GACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTT                                                                                                       >  SRR19973065.1343954/1‑150 (MQ=60)
                                               GACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTT                                                                                                       >  SRR19973065.585272/1‑150 (MQ=60)
                                                ACTTTTCAGATGACATACATTCCGGGTACCTACAACAATGGAAATGTGTACTTTGCCTGGATGCGATTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTT                                                                                                      <  SRR19973065.215375/150‑1 (MQ=60)
                                                 CTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTT                                                                                                     >  SRR19973065.1478358/1‑150 (MQ=60)
                                                    TTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTTAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATC                                                                                                  <  SRR19973065.3360406/150‑1 (MQ=60)
                                                    TTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATC                                                                                                  >  SRR19973065.2385113/1‑150 (MQ=60)
                                                      CAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCAC                                                                                                <  SRR19973065.3189033/150‑1 (MQ=60)
                                                       AGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCATTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACT                                                                                               <  SRR19973065.3321432/150‑1 (MQ=60)
                                                         ATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTT                                                                                             <  SRR19973065.2514754/150‑1 (MQ=60)
                                                         ATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTT                                                                                             <  SRR19973065.1093268/150‑1 (MQ=60)
                                                         ATGACCTACATTCCGGGTACCTACAACAAGGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAATTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTT                                                                                             <  SRR19973065.1093264/150‑1 (MQ=60)
                                                          TGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTT                                                                                            >  SRR19973065.2998164/1‑150 (MQ=60)
                                                           GACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCCATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTT                                                                                           >  SRR19973065.3042805/1‑150 (MQ=60)
                                                              CTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGT                                                                                        >  SRR19973065.238211/1‑150 (MQ=60)
                                                                  tgTCCGGGTACCTACAAAACTCGAAATTTGTACTTTGCCTGGATGCGCTTTCAGTTTTTAGACATATGTATTTTTAGTAACTTTTTTAAAATCAATAGATAAAATAAGTAAAATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAG                                                                                    <  SRR19973065.980363/148‑1 (MQ=60)
                                                                  ATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAG                                                                                    <  SRR19973065.980500/150‑1 (MQ=60)
                                                                      CGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTAC                                                                                >  SRR19973065.2829937/1‑150 (MQ=60)
                                                                      CGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTAC                                                                                >  SRR19973065.3223849/1‑150 (MQ=60)
                                                                         GTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACT                                                                             <  SRR19973065.262158/150‑1 (MQ=60)
                                                                          TACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTG                                                                            >  SRR19973065.745665/1‑150 (MQ=60)
                                                                          TACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTG                                                                            >  SRR19973065.744457/1‑150 (MQ=60)
                                                                           ACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGG                                                                           >  SRR19973065.1729878/1‑150 (MQ=60)
                                                                           ACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGG                                                                           >  SRR19973065.3393402/1‑150 (MQ=60)
                                                                             CTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGAC                                                                         >  SRR19973065.753331/1‑150 (MQ=60)
                                                                               ACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAA                                                                       >  SRR19973065.578778/1‑150 (MQ=60)
                                                                                   CCATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATAACTTTTTAGTAAAGTTACACTGGACAAAGCG                                                                   <  SRR19973065.2975276/150‑1 (MQ=60)
                                                                                   CAATTGAAATGTGTACTTTGCCTGGATGCTCTTTCAGTTTTGAGACAAATTAAGTTTTAGTAAATTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATAACTTTTTAGTAAAGTTACACTGGACAAAGCG                                                                   <  SRR19973065.2975281/150‑1 (MQ=60)
                                                                                     ATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTA                                                                 >  SRR19973065.756448/1‑150 (MQ=60)
                                                                                     ATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTA                                                                 >  SRR19973065.756447/1‑150 (MQ=60)
                                                                                     ATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTA                                                                 >  SRR19973065.756267/1‑150 (MQ=60)
                                                                                      TGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAATGCGACTTTTATCACTTTTTATTAAAGTTACACTGGACAAAGCGTAC                                                                >  SRR19973065.3113137/1‑150 (MQ=60)
                                                                                      TGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTAC                                                                <  SRR19973065.758128/150‑1 (MQ=60)
                                                                                      TGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTAC                                                                <  SRR19973065.1780402/150‑1 (MQ=60)
                                                                                                 ACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTG                                                     <  SRR19973065.2354905/150‑1 (MQ=60)
                                                                                                  CTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGT                                                    >  SRR19973065.2720121/1‑150 (MQ=60)
                                                                                                  CTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGT                                                    >  SRR19973065.510075/1‑150 (MQ=60)
                                                                                                  CTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGT                                                    >  SRR19973065.485036/1‑150 (MQ=60)
                                                                                                  CTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGT                                                    >  SRR19973065.3501689/1‑150 (MQ=60)
                                                                                                  CTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGT                                                    >  SRR19973065.466433/1‑150 (MQ=60)
                                                                                                  CTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGT                                                    >  SRR19973065.1956/1‑150 (MQ=60)
                                                                                                          GGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAA                                            >  SRR19973065.3474896/1‑150 (MQ=60)
                                                                                                             TGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCG                                         >  SRR19973065.169510/1‑150 (MQ=60)
                                                                                                                GCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACA                                      <  SRR19973065.2657989/150‑1 (MQ=60)
                                                                                                                GCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAAAGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGAGTACCACAATAGGTGTAGTGGTAACCGACA                                      >  SRR19973065.2493873/1‑150 (MQ=60)
                                                                                                                      AGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCAT                                >  SRR19973065.2745617/1‑150 (MQ=60)
                                                                                                                           TGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTG                           <  SRR19973065.880726/150‑1 (MQ=60)
                                                                                                                               ACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTGGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTA                       <  SRR19973065.3305664/150‑1 (MQ=60)
                                                                                                                                 AAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATT                     >  SRR19973065.2098167/1‑150 (MQ=60)
                                                                                                                                   ATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTT                   >  SRR19973065.2059822/1‑150 (MQ=60)
                                                                                                                                     GAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTC                 <  SRR19973065.3157191/150‑1 (MQ=60)
                                                                                                                                     GAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTC                 >  SRR19973065.2809106/1‑150 (MQ=60)
                                                                                                                                      AAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCA                <  SRR19973065.2571553/150‑1 (MQ=60)
                                                                                                                                      AAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCA                >  SRR19973065.2712208/1‑150 (MQ=60)
                                                                                                                                       AGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCAT               <  SRR19973065.1999612/150‑1 (MQ=60)
                                                                                                                                        GTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATG              >  SRR19973065.2444621/1‑150 (MQ=60)
                                                                                                                                           TTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAA           <  SRR19973065.2529640/150‑1 (MQ=60)
                                                                                                                                                AACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTA      >  SRR19973065.3359793/1‑150 (MQ=60)
                                                                                                                                                  CTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTagatcggaagagcacacgtctgaactc    >  SRR19973065.1327766/1‑123 (MQ=60)
                                                                                                                                                   TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATT   >  SRR19973065.2933311/1‑150 (MQ=60)
                                                                                                                                                    TTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTT  >  SRR19973065.1480995/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTT  >  CP009273/652448‑652745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑