Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 652,597 C→T 100% intergenic (+24/‑151) pagP → / → cspE phospholipid:lipid A palmitoyltransferase/constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273652,5970CT97.5% 662.2 / 4.7 201intergenic (+24/‑151)pagP/cspEphospholipid:lipid A palmitoyltransferase/constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base T (103/93);  total (105/96)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 207 total aligned reads displayed.

CCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTA  >  CP009273/652454‑652741
                                                                                                                                                |                                                                                                                                                
ccGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAAC‑TTTTTTa                                                                                                                                              >  1:3520338/1‑148 (MQ=255)
 cGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTaa                                                                                                                                             >  2:1459017/1‑148 (MQ=255)
  ggTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAAC‑TTTTTTaaa                                                                                                                                            <  1:862581/148‑1 (MQ=255)
  ggTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTaaa                                                                                                                                            <  2:1442146/148‑1 (MQ=255)
  ggTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTaaa                                                                                                                                            <  1:3713087/148‑1 (MQ=255)
   gTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTaaaa                                                                                                                                           <  1:2491162/148‑1 (MQ=255)
    ttCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAAt                                                                                                                                          <  1:2940405/148‑1 (MQ=255)
     tCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATc                                                                                                                                         >  1:486966/1‑148 (MQ=255)
     tCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATc                                                                                                                                         >  1:1769548/1‑148 (MQ=255)
      cTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCa                                                                                                                                        <  1:2078493/148‑1 (MQ=255)
       tACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTNACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGNGTNCTTTGCCTGGATGCGCTTTCAGTTNTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCaa                                                                                                                                       <  1:2242996/148‑1 (MQ=255)
       tACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCaa                                                                                                                                       <  2:786406/148‑1 (MQ=255)
       tACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCaa                                                                                                                                       <  2:408099/148‑1 (MQ=255)
          tGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAg                                                                                                                                    <  2:1425207/148‑1 (MQ=255)
              aTTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTaa                                                                                                                                >  2:3510769/1‑148 (MQ=255)
                   ccTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATaa                                                                                                                           <  1:3285159/148‑1 (MQ=255)
                       cGTGGGTTATGGCCCAGTGACATTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCATTGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTaa                                                                                                                       <  2:3477885/148‑1 (MQ=255)
                         tGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACa                                                                                                                     >  1:34168/1‑148 (MQ=255)
                          gggTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACAt                                                                                                                    <  1:3441173/148‑1 (MQ=255)
                              tATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCaaa                                                                                                                >  2:2891686/1‑148 (MQ=255)
                              tATGGCCCAGTGACTTTTCAGATGACCTACATACCGGGTACCTACAACAATGGCAATTTGTACTATGCCTGGATGCGCTATCAGTTTTGAGACAAATAAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAAGAAGTAACATCaaa                                                                                                                >  1:1002407/1‑148 (MQ=255)
                               aTGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCaaaa                                                                                                               >  1:3171779/1‑148 (MQ=255)
                                tGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCaaaaa                                                                                                              <  1:1278091/148‑1 (MQ=255)
                                  gCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATa                                                                                                            <  2:314714/148‑1 (MQ=255)
                                   cccAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATaa                                                                                                           <  1:164328/148‑1 (MQ=255)
                                     cAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAAcg                                                                                                         >  2:1458445/1‑148 (MQ=255)
                                        tGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGa                                                                                                      >  1:1335750/1‑148 (MQ=255)
                                        tGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGa                                                                                                      >  1:3691262/1‑148 (MQ=255)
                                         gACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGAc                                                                                                     >  1:2609406/1‑148 (MQ=255)
                                            ttttCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACttt                                                                                                  >  2:409888/1‑148 (MQ=255)
                                            ttttCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACttt                                                                                                  <  1:3247737/148‑1 (MQ=255)
                                             tttCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACtttt                                                                                                 <  1:801662/148‑1 (MQ=255)
                                             tttCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACtttt                                                                                                 >  1:1413920/1‑148 (MQ=255)
                                                  gATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCa                                                                                             <  1:879210/148‑1 (MQ=255)
                                                    tGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACtt                                                                                          <  2:665699/148‑1 (MQ=255)
                                                     gACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACttt                                                                                         <  2:2227696/148‑1 (MQ=255)
                                                     gACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACttt                                                                                         >  2:3653338/1‑148 (MQ=255)
                                                       ccTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACttttt                                                                                       <  1:2327730/148‑1 (MQ=255)
                                                         tACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAg                                                                                     <  1:2495851/148‑1 (MQ=255)
                                                          aCATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGt                                                                                    >  2:1906508/1‑148 (MQ=255)
                                                          aCATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGt                                                                                    >  2:2581021/1‑148 (MQ=255)
                                                           cATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTa                                                                                   >  1:3695798/1‑148 (MQ=255)
                                                            aTTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTaa                                                                                  <  2:827923/148‑1 (MQ=255)
                                                            aTTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTaa                                                                                  >  1:607408/1‑148 (MQ=255)
                                                             ttCCGGGTACCTACAACACTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTaaa                                                                                 <  1:1878499/148‑1 (MQ=255)
                                                             ttCCGGGTACCTACAACAATGGAAATGTTTTCTTTGCCTGGATGCGCGTTCAGTTTTGATACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTaaa                                                                                 <  1:3767885/148‑1 (MQ=255)
                                                              tCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAg                                                                                >  1:2400177/1‑148 (MQ=255)
                                                                 gggTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTa                                                                             <  2:3349706/148‑1 (MQ=255)
                                                                  ggTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTac                                                                            <  1:1222032/148‑1 (MQ=255)
                                                                  ggTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTac                                                                            >  1:380375/1‑148 (MQ=255)
                                                                      ccTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTg                                                                        >  1:1308522/1‑148 (MQ=255)
                                                                            acaaTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACaaa                                                                  <  1:3365174/148‑1 (MQ=255)
                                                                            acaaTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACaaa                                                                  <  1:3366748/148‑1 (MQ=255)
                                                                             caaTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTTAAGTTACACTGGAGAAAg                                                                 >  2:3168957/1‑148 (MQ=255)
                                                                             caaTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACTCTGGACAAAg                                                                 >  2:1933669/1‑148 (MQ=255)
                                                                              aaTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGc                                                                >  2:3732831/1‑148 (MQ=255)
                                                                                  gCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTAc                                                            <  1:3107649/148‑1 (MQ=255)
                                                                                      tgtgTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACcaca                                                        <  2:2099065/148‑1 (MQ=255)
                                                                                      tgtgTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACcacc                                                        >  2:3663883/1‑147 (MQ=255)
                                                                                       gtgtACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACaa                                                       <  1:718080/148‑1 (MQ=255)
                                                                                           aCTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTgg                                                   >  2:489483/1‑148 (MQ=255)
                                                                                            cTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGgt                                                  >  2:1897196/1‑148 (MQ=255)
                                                                                            cTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGgt                                                  >  1:3712303/1‑148 (MQ=255)
                                                                                              ttGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGgtgt                                                >  1:1669895/1‑148 (MQ=255)
                                                                                               tGCCTGGATGCGCTTTCAGTTTTGAGACGAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTa                                               <  2:932186/148‑1 (MQ=255)
                                                                                               tGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTa                                               <  2:1589012/148‑1 (MQ=255)
                                                                                               tGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTa                                               >  2:1166606/1‑148 (MQ=255)
                                                                                                 ccTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACt                                             <  1:1059878/148‑1 (MQ=255)
                                                                                                   tGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTgg                                           >  1:1414793/1‑148 (MQ=255)
                                                                                                   tGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTgg                                           >  1:2274793/1‑148 (MQ=255)
                                                                                                    ggATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGt                                          >  1:1892856/1‑148 (MQ=255)
                                                                                                         cgcTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCg                                     <  2:1235820/148‑1 (MQ=255)
                                                                                                          gcTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGa                                    <  2:2113944/148‑1 (MQ=255)
                                                                                                           cTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGac                                   >  1:2375634/1‑148 (MQ=255)
                                                                                                            tttCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGaca                                  <  2:3780414/148‑1 (MQ=255)
                                                                                                                 gTTTTGATACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCa                             <  2:724977/148‑1 (MQ=255)
                                                                                                                  ttttGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCAt                            >  2:2633828/1‑148 (MQ=255)
                                                                                                                   tttGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCAtt                           >  2:2992159/1‑148 (MQ=255)
                                                                                                                     tGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTtg                         <  1:1514605/148‑1 (MQ=255)
                                                                                                                       ataCAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTtgtg                       >  2:3172836/3‑148 (MQ=255)
                                                                                                                         aCAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTc                     >  2:1732410/1‑148 (MQ=255)
                                                                                                                         aCAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCAGTTGTGTc                     >  2:3384456/1‑148 (MQ=255)
                                                                                                                          cAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCt                    <  1:155876/148‑1 (MQ=255)
                                                                                                                           aaaTGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCCTTTGTGTCTa                   >  2:3743003/1‑148 (MQ=255)
                                                                                                                           aaaTGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTa                   >  2:1852477/1‑148 (MQ=255)
                                                                                                                            aaTGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAt                  <  1:890970/148‑1 (MQ=255)
                                                                                                                            aaTGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAt                  <  1:2456991/148‑1 (MQ=255)
                                                                                                                             aTGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAtt                 >  2:1506631/1‑148 (MQ=255)
                                                                                                                              tGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAttt                >  1:47756/1‑148 (MQ=255)
                                                                                                                              tGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAttt                >  1:2858976/1‑148 (MQ=255)
                                                                                                                              tGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAttt                >  1:947923/1‑148 (MQ=255)
                                                                                                                              tGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAttt                >  1:2145069/1‑148 (MQ=255)
                                                                                                                                   ttttAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCAt           <  1:3116868/148‑1 (MQ=255)
                                                                                                                                   ttttAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCAt           >  2:2353864/1‑148 (MQ=255)
                                                                                                                                    tttAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATg          >  2:958088/1‑148 (MQ=255)
                                                                                                                                       aGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTaa       >  1:1617569/1‑148 (MQ=255)
                                                                                                                                       aGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTaa       <  1:3566932/148‑1 (MQ=255)
                                                                                                                                         tAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAg     >  1:1812041/1‑148 (MQ=255)
                                                                                                                                         tAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAg     >  2:2711366/1‑148 (MQ=255)
                                                                                                                                            c‑tttttTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTa  >  2:1683740/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTA  >  CP009273/652454‑652741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATT  >  CP009273/652448‑652744
                                                                                                                                                      |                                                                                                                                                   
CCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTT                                                                                                                                                     <  SRR19973069.3361824/150‑1 (MQ=60)
CCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTT                                                                                                                                                     <  SRR19973069.3359531/150‑1 (MQ=60)
CCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTT                                                                                                                                                     <  SRR19973069.2564754/150‑1 (MQ=60)
   CTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTT                                                                                                                                                  <  SRR19973069.908395/150‑1 (MQ=60)
    TCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAAC‑TTTTTTA                                                                                                                                                 >  SRR19973069.3543082/1‑150 (MQ=60)
        GGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAAC‑TTTTTTAAAAT                                                                                                                                             <  SRR19973069.868606/150‑1 (MQ=60)
        GGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAAC‑TTTTTTAAAAT                                                                                                                                             <  SRR19973069.868607/150‑1 (MQ=60)
        GGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAAC‑TTTTTTAAAAT                                                                                                                                             <  SRR19973069.868677/150‑1 (MQ=60)
        GGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAAT                                                                                                                                             <  SRR19973069.2500231/150‑1 (MQ=60)
        GGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAAT                                                                                                                                             <  SRR19973069.1835356/150‑1 (MQ=60)
        GGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAAT                                                                                                                                             <  SRR19973069.3737339/150‑1 (MQ=60)
         GTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATC                                                                                                                                            <  SRR19973069.2507825/150‑1 (MQ=60)
         GTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATC                                                                                                                                            >  SRR19973069.1782018/1‑150 (MQ=60)
         GTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATC                                                                                                                                            <  SRR19973069.164108/150‑1 (MQ=60)
         GTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATC                                                                                                                                            >  SRR19973069.490480/1‑150 (MQ=60)
          TTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCA                                                                                                                                           <  SRR19973069.2959907/150‑1 (MQ=60)
            CTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAAT                                                                                                                                         <  SRR19973069.3278691/150‑1 (MQ=60)
            CTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAAT                                                                                                                                         <  SRR19973069.2092877/150‑1 (MQ=60)
             TACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTNACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGNGTNCTTTGCCTGGATGCGCTTTCAGTTNTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATA                                                                                                                                        <  SRR19973069.2258105/150‑1 (MQ=60)
             TACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCATATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATA                                                                                                                                        <  SRR19973069.2258631/150‑1 (MQ=60)
             TACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATA                                                                                                                                        <  SRR19973069.2430094/150‑1 (MQ=60)
                TGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCT                                                                                                                                     <  SRR19973069.1893699/150‑1 (MQ=60)
                       GGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAA                                                                                                                              <  SRR19973069.2253524/150‑1 (MQ=60)
                       GGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAA                                                                                                                              >  SRR19973069.3524622/1‑150 (MQ=60)
                         CCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGT                                                                                                                            <  SRR19973069.1363085/150‑1 (MQ=60)
                         CCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGT                                                                                                                            <  SRR19973069.3306679/150‑1 (MQ=60)
                             CGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACA                                                                                                                        <  SRR19973069.2304143/150‑1 (MQ=60)
                             CGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACA                                                                                                                        >  SRR19973069.892274/1‑150 (MQ=60)
                             CGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACA                                                                                                                        >  SRR19973069.34650/1‑150 (MQ=60)
                                GGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCA                                                                                                                     <  SRR19973069.3463510/150‑1 (MQ=60)
                                GGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCA                                                                                                                     <  SRR19973069.3579406/150‑1 (MQ=60)
                                 GGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAA                                                                                                                    >  SRR19973069.2045017/1‑150 (MQ=60)
                                  GTTATGGCCCAGTGACTTTTCAGATGACCTACATACCGGGTACCTACAACAATGGCAATTTGTACTATGCCTGGATGCGCTATCAGTTTTGAGACAAATAAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAAGAAGTAACATCAAA                                                                                                                   >  SRR19973069.1009285/1‑150 (MQ=60)
                                   TTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAA                                                                                                                  >  SRR19973069.3192439/1‑150 (MQ=60)
                                     ATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAAT                                                                                                                <  SRR19973069.1820830/150‑1 (MQ=60)
                                     ATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAAT                                                                                                                <  SRR19973069.3722357/150‑1 (MQ=60)
                                      TGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATA                                                                                                               <  SRR19973069.1286661/150‑1 (MQ=60)
                                         CCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACG                                                                                                            <  SRR19973069.165748/150‑1 (MQ=60)
                                            AGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGA                                                                                                         >  SRR19973069.3715401/1‑150 (MQ=60)
                                            AGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGA                                                                                                         >  SRR19973069.1344967/1‑150 (MQ=60)
                                             GTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGAC                                                                                                        <  SRR19973069.3625196/150‑1 (MQ=60)
                                             GTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGAC                                                                                                        >  SRR19973069.2626854/1‑150 (MQ=60)
                                                 CTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTT                                                                                                    <  SRR19973069.1567149/150‑1 (MQ=60)
                                                 CTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTT                                                                                                    >  SRR19973069.1423773/1‑150 (MQ=60)
                                                  TTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTA                                                                                                   <  SRR19973069.3269001/150‑1 (MQ=60)
                                                   TTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTAT                                                                                                  <  SRR19973069.807346/150‑1 (MQ=60)
                                                    TTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATC                                                                                                 <  SRR19973069.239250/150‑1 (MQ=60)
                                                       AGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACT                                                                                              >  SRR19973069.726152/1‑150 (MQ=60)
                                                        GATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACT                                                                                              <  SRR19973069.885311/150‑1 (MQ=60)
                                                          TGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTT                                                                                           <  SRR19973069.1050763/150‑1 (MQ=60)
                                                           GACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTT                                                                                          >  SRR19973069.1304498/1‑150 (MQ=60)
                                                             CCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAG                                                                                        <  SRR19973069.2343559/150‑1 (MQ=60)
                                                               TACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTA                                                                                      >  SRR19973069.3719957/1‑150 (MQ=60)
                                                               TACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTA                                                                                      <  SRR19973069.2512047/150‑1 (MQ=60)
                                                               TACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTA                                                                                      <  SRR19973069.2512537/150‑1 (MQ=60)
                                                               TACATTCCGCGTACCTACAACAATGGCAATGTGTACTTTGCCAGGATGCGCTTTAAGTATTGATACAAATGAATTTTTATTAAC‑AATTTTAAAATCAATATATAAAATAAGTAACATCAAAAATAAagatactattataactctttagaa                                                                                      >  SRR19973069.3719827/1‑126 (MQ=60)
                                                                ACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAA                                                                                     >  SRR19973069.611486/1‑150 (MQ=60)
                                                                ACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAA                                                                                     >  SRR19973069.610619/1‑150 (MQ=60)
                                                                  ATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAG                                                                                   >  SRR19973069.2416427/1‑150 (MQ=60)
                                                                   TTCCGGGTACCTACAACACTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGT                                                                                  <  SRR19973069.1891562/150‑1 (MQ=60)
                                                                   TTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGT                                                                                  <  SRR19973069.1745595/150‑1 (MQ=60)
                                                                   TTCCGGGTACCTACAACAATGGAAATGTTTTCTTTGCCTGGATGCGCGTTCAGTTTTGATACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGT                                                                                  <  SRR19973069.3792368/150‑1 (MQ=60)
                                                                      CGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTAC                                                                               >  SRR19973069.383151/1‑150 (MQ=60)
                                                                        GGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACAC                                                                             <  SRR19973069.1230386/150‑1 (MQ=60)
                                                                          TACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTG                                                                           >  SRR19973069.1317459/1‑150 (MQ=60)
                                                                                CAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAA                                                                     >  SRR19973069.3105925/1‑150 (MQ=60)
                                                                                 AACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAG                                                                    >  SRR19973069.3445658/1‑150 (MQ=60)
                                                                                  ACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGC                                                                   <  SRR19973069.3387187/150‑1 (MQ=60)
                                                                                  ACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGC                                                                   <  SRR19973069.3388764/150‑1 (MQ=60)
                                                                                        GCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCA                                                             <  SRR19973069.3128094/150‑1 (MQ=60)
                                                                                         CAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCAC                                                            >  SRR19973069.1201052/1‑150 (MQ=60)
                                                                                          AATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACA                                                           >  SRR19973069.1341033/1‑150 (MQ=60)
                                                                                             GTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATT                                                        <  SRR19973069.723139/150‑1 (MQ=60)
                                                                                             GTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATT                                                        >  SRR19973069.1882473/1‑150 (MQ=60)
                                                                                               GTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGG                                                      <  SRR19973069.1502960/150‑1 (MQ=60)
                                                                                                TACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGT                                                     >  SRR19973069.3736551/1‑150 (MQ=60)
                                                                                                TACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGT                                                     >  SRR19973069.3736566/1‑150 (MQ=60)
                                                                                                  CTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGT                                                   >  SRR19973069.1681492/1‑150 (MQ=60)
                                                                                                  CTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTAAACTGGACAAAGCGTACCACAATTGGTGT                                                   >  SRR19973069.1679912/1‑150 (MQ=60)
                                                                                                  CTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACAACAATTGGTGT                                                   >  SRR19973069.2882519/1‑150 (MQ=60)
                                                                                                       CCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGG                                              <  SRR19973069.1067246/150‑1 (MQ=60)
                                                                                                       CCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGG                                              >  SRR19973069.2290228/1‑150 (MQ=60)
                                                                                                       CCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGG                                              >  SRR19973069.1424634/1‑150 (MQ=60)
                                                                                                       CCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGG                                              >  SRR19973069.1424653/1‑150 (MQ=60)
                                                                                                        CTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGT                                             <  SRR19973069.3774917/150‑1 (MQ=60)
                                                                                                        CTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGT                                             >  SRR19973069.1905975/1‑150 (MQ=60)
                                                                                                         TGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTA                                            <  SRR19973069.2910749/150‑1 (MQ=60)
                                                                                                           GATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAAC                                          >  SRR19973069.41904/1‑150 (MQ=60)
                                                                                                               CGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGAC                                      >  SRR19973069.2391750/1‑150 (MQ=60)
                                                                                                               CGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGAC                                      >  SRR19973069.2391938/1‑150 (MQ=60)
                                                                                                                   TTCAGTTTTGAGACAAATTAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAG                                  <  SRR19973069.3218380/150‑1 (MQ=60)
                                                                                                                           TGAGACAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTG                          <  SRR19973069.1525067/150‑1 (MQ=60)
                                                                                                                                CAAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAT                     <  SRR19973069.157247/150‑1 (MQ=60)
                                                                                                                                 AAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATT                    >  SRR19973069.3134849/1‑150 (MQ=60)
                                                                                                                                 AAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATT                    <  SRR19973069.903348/150‑1 (MQ=60)
                                                                                                                                 AAATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATT                    <  SRR19973069.3432412/150‑1 (MQ=60)
                                                                                                                                  AATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTT                   >  SRR19973069.2159753/1‑150 (MQ=60)
                                                                                                                                  AATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTT                   >  SRR19973069.2159788/1‑150 (MQ=60)
                                                                                                                                  AATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTT                   >  SRR19973069.954305/1‑150 (MQ=60)
                                                                                                                                  AATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTT                   <  SRR19973069.897117/150‑1 (MQ=60)
                                                                                                                                  AATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTT                   >  SRR19973069.48377/1‑150 (MQ=60)
                                                                                                                                  AATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTT                   <  SRR19973069.2473500/150‑1 (MQ=60)
                                                                                                                                  AATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTT                   >  SRR19973069.48357/1‑150 (MQ=60)
                                                                                                                                  AATGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTT                   >  SRR19973069.2877776/1‑150 (MQ=60)
                                                                                                                                  AATGAAGTTTTAGTAAC‑TTTTTTAAAATCAACAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTT                   >  SRR19973069.630085/1‑150 (MQ=60)
                                                                                                                                    TGAAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTT                 >  SRR19973069.3818324/1‑150 (MQ=60)
                                                                                                                                      AAGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCA               >  SRR19973069.3354603/1‑150 (MQ=60)
                                                                                                                                       AGTTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCAT              >  SRR19973069.3161838/1‑150 (MQ=60)
                                                                                                                                         TTTTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGT            <  SRR19973069.3137351/150‑1 (MQ=60)
                                                                                                                                           TTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAA          >  SRR19973069.2599821/1‑150 (MQ=60)
                                                                                                                                           TTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAA          >  SRR19973069.1628503/1‑150 (MQ=60)
                                                                                                                                           TTAGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAA          >  SRR19973069.1628224/1‑150 (MQ=60)
                                                                                                                                             AGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAG        >  SRR19973069.3730940/1‑150 (MQ=60)
                                                                                                                                             AGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAG        >  SRR19973069.1824779/1‑150 (MQ=60)
                                                                                                                                             AGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAG        <  SRR19973069.3590158/150‑1 (MQ=60)
                                                                                                                                             AGTAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAG        <  SRR19973069.1238723/150‑1 (MQ=60)
                                                                                                                                               TAAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGT      >  SRR19973069.2665723/1‑150 (MQ=60)
                                                                                                                                                AAC‑TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTA     >  SRR19973069.3388653/1‑150 (MQ=60)
                                                                                                                                                    TTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATT  >  SRR19973069.3227334/1‑150 (MQ=60)
                                                                                                                                                      |                                                                                                                                                   
CCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAAC‑TTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATT  >  CP009273/652448‑652744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑