Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 652,597 C→T 100% intergenic (+24/‑151) pagP → / → cspE phospholipid:lipid A palmitoyltransferase/constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273652,5970CT93.9% 713.0 / 25.1 226intergenic (+24/‑151)pagP/cspEphospholipid:lipid A palmitoyltransferase/constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (1/13);  new base T (107/105);  total (108/118)
Fisher's exact test for biased strand distribution p-value = 1.54e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 236 total aligned reads displayed.

TCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAA  >  CP009273/652450‑652742
                                                                                                                                                   |                                                                                                                                                 
tctcCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAActtc                                                                                                                                                   <  2:2714484/148‑1 (MQ=255)
  tcCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAActtctt                                                                                                                                                 >  1:1094638/1‑148 (MQ=255)
    ccGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTa                                                                                                                                               <  1:799614/148‑1 (MQ=255)
    ccGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTa                                                                                                                                               >  1:975250/1‑148 (MQ=255)
      ggTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTaaa                                                                                                                                             <  1:1043908/148‑1 (MQ=255)
      ggTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTaaa                                                                                                                                             >  2:2079334/1‑148 (MQ=255)
      ggTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTaaa                                                                                                                                             <  1:1724234/148‑1 (MQ=255)
      ggTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTaaa                                                                                                                                             <  2:3246750/148‑1 (MQ=255)
       gTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTaaaa                                                                                                                                            <  2:3050396/148‑1 (MQ=255)
          cTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCa                                                                                                                                         >  2:2385095/1‑148 (MQ=255)
          cTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCa                                                                                                                                         >  2:279406/1‑148 (MQ=255)
            aCTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAAt                                                                                                                                       <  1:3219349/148‑1 (MQ=255)
             cTGCCATTGGCCTCCGTGGGTTATGGCTCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACTAATGAAGTTTTAGTAACTTTTTTTAAATCAATa                                                                                                                                      <  2:575515/148‑1 (MQ=255)
             cTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATa                                                                                                                                      <  2:1211908/148‑1 (MQ=255)
              tGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAg                                                                                                                                     >  1:170599/1‑148 (MQ=255)
                 cATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTa                                                                                                                                  >  2:3070229/1‑148 (MQ=255)
                   ttGTCCTCCGTGGGTTATGGCCAAGTGACTTTTCATATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTaaa                                                                                                                                <  2:426447/148‑1 (MQ=255)
                   ttGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTaaa                                                                                                                                >  2:1820430/1‑148 (MQ=255)
                   ttGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTaaa                                                                                                                                >  1:3281421/1‑148 (MQ=255)
                   ttGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTaaa                                                                                                                                <  1:2480891/148‑1 (MQ=255)
                    tGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTCCCTACAACAATGGCCATGTGTACTTTGACTGGATGCGCTTTCAGTTTTGTGACAAATGAAGTTTTAGTAACTTTTGTAAAATCAATAGCTaaaa                                                                                                                               >  2:233852/1‑148 (MQ=255)
                    tGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATATCTaaaa                                                                                                                               >  2:233683/1‑148 (MQ=255)
                    tGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTaaaa                                                                                                                               <  2:1649528/148‑1 (MQ=255)
                    tGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTaaaa                                                                                                                               >  1:3059557/1‑148 (MQ=255)
                     ggCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAAt                                                                                                                              <  2:923737/148‑1 (MQ=255)
                     ggCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAAt                                                                                                                              >  2:451945/1‑148 (MQ=255)
                      gCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATa                                                                                                                             <  1:3225322/148‑1 (MQ=255)
                      gCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATa                                                                                                                             <  2:221250/148‑1 (MQ=255)
                          ccGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTa                                                                                                                         <  1:2847393/148‑1 (MQ=255)
                              gggTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACAt                                                                                                                     >  1:2572817/1‑148 (MQ=255)
                              gggTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACAt                                                                                                                     >  1:2912013/1‑148 (MQ=255)
                              gggTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACAt                                                                                                                     >  1:314580/1‑148 (MQ=255)
                                tttATGGCCCAGTGAATTTTCAGATGACCTACATTCCGGGTACCTACACCCATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTTACATCa                                                                                                                   <  2:3422363/147‑1 (MQ=255)
                                  tATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCaaa                                                                                                                 >  1:861249/1‑148 (MQ=255)
                                  tATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCaaa                                                                                                                 <  1:2776594/148‑1 (MQ=255)
                                  tATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCaaa                                                                                                                 <  2:2640980/148‑1 (MQ=255)
                                   aTGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCaaaa                                                                                                                <  2:1314651/148‑1 (MQ=255)
                                    tGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCaaaaa                                                                                                               >  1:2303675/1‑148 (MQ=255)
                                    tGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCaaaaa                                                                                                               <  2:102658/148‑1 (MQ=255)
                                      gCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATa                                                                                                             <  2:366071/148‑1 (MQ=255)
                                      gCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATa                                                                                                             <  1:1804579/148‑1 (MQ=255)
                                         cAGTGACTTTTCAGATGACCTACATTCCGTGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATATCTAAAATAAGTAACATCAAAAATAAcg                                                                                                          >  1:2337199/1‑148 (MQ=255)
                                         cAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAAcg                                                                                                          <  1:2609879/148‑1 (MQ=255)
                                               cTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACtt                                                                                                    >  1:1932852/1‑148 (MQ=255)
                                                ttttCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACttt                                                                                                   >  2:2983545/1‑148 (MQ=255)
                                                 tttCAGATGACCTACATTCCGGGTACCTACAACAAGGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACtttt                                                                                                  <  2:2223695/148‑1 (MQ=255)
                                                   tCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTAt                                                                                                <  2:2771010/148‑1 (MQ=255)
                                                    cAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAATTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATc                                                                                               <  1:987018/148‑1 (MQ=255)
                                                           ccTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACttttt                                                                                        >  1:239710/1‑148 (MQ=255)
                                                           ccTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACttttt                                                                                        >  1:1824501/1‑148 (MQ=255)
                                                               cATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTa                                                                                    >  1:938501/1‑148 (MQ=255)
                                                                aTTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTaa                                                                                   <  1:2540922/148‑1 (MQ=255)
                                                                 ttCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTaaa                                                                                  <  1:395878/148‑1 (MQ=255)
                                                                  tCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAg                                                                                 <  2:1574105/148‑1 (MQ=255)
                                                                      ggTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTac                                                                             >  2:3391965/1‑148 (MQ=255)
                                                                        tACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTGTTATCACTTTTTAGTAAAGTTacac                                                                           >  1:1843580/1‑148 (MQ=255)
                                                                           cTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAACAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTgg                                                                        >  2:1134242/1‑148 (MQ=255)
                                                                           cTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTgg                                                                        >  2:2084544/1‑148 (MQ=255)
                                                                            tACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGa                                                                       >  2:1696437/1‑148 (MQ=255)
                                                                              caacaaTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACa                                                                     >  2:2484618/1‑148 (MQ=255)
                                                                               aacaaGGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACaa                                                                    <  1:303757/148‑1 (MQ=255)
                                                                                acaaTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACaaa                                                                   >  2:1203030/1‑148 (MQ=255)
                                                                                  aaTGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGc                                                                 <  1:2079334/148‑1 (MQ=255)
                                                                                        aaTGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACca                                                           >  1:1404794/1‑148 (MQ=255)
                                                                                        aaTGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACca                                                           >  1:3179295/1‑148 (MQ=255)
                                                                                          tgtgTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACcaca                                                         >  1:2159116/1‑148 (MQ=255)
                                                                                           gtgtACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACaa                                                        <  1:1456208/148‑1 (MQ=255)
                                                                                             gtACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAAtt                                                      <  1:1173891/148‑1 (MQ=255)
                                                                                               aCTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTgg                                                    <  1:1788828/148‑1 (MQ=255)
                                                                                                    gCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTAc                                               <  2:3427104/148‑1 (MQ=255)
                                                                                                      cTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTg                                             >  2:2274645/1‑148 (MQ=255)
                                                                                                       tGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTgg                                            >  1:1628192/1‑148 (MQ=255)
                                                                                                         gATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTa                                          <  2:68207/148‑1 (MQ=255)
                                                                                                         gATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTa                                          >  2:3290450/1‑148 (MQ=255)
                                                                                                             cgcTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCg                                      <  1:1253512/148‑1 (MQ=255)
                                                                                                             cgcTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCg                                      <  2:2868849/148‑1 (MQ=255)
                                                                                                               cTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGac                                    >  2:523769/1‑148 (MQ=255)
                                                                                                               cTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGac                                    <  2:3531464/148‑1 (MQ=255)
                                                                                                                tttCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGaca                                   >  2:777264/1‑148 (MQ=255)
                                                                                                                 ttCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGacac                                  >  1:3081136/1‑148 (MQ=255)
                                                                                                                    aGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACAACAATTGGTGTACTGGTAACCGACACAGc                               <  1:1363955/148‑1 (MQ=255)
                                                                                                                     gTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCa                              <  1:2633699/148‑1 (MQ=255)
                                                                                                                      ttttGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCAt                             >  2:1322545/1‑148 (MQ=255)
                                                                                                                      ttttGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCAt                             >  2:2546169/1‑148 (MQ=255)
                                                                                                                      ttttGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCAt                             >  1:499619/1‑148 (MQ=255)
                                                                                                                               aaaTGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTa                    >  1:1268566/1‑148 (MQ=255)
                                                                                                                                aaTGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAt                   >  2:2922406/1‑148 (MQ=255)
                                                                                                                                aaTGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAt                   <  2:2303675/148‑1 (MQ=255)
                                                                                                                                 aTGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCAGTTGTTAGTAAAGTTACTCTGGACAAAGCGTACCACAAGTGGGGTCCTGGGGTCCGACACAGCCTGTGTGTCTAtt                  >  2:1961445/1‑148 (MQ=255)
                                                                                                                                    aaGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAttttt               <  2:808943/148‑1 (MQ=255)
                                                                                                                                    aaGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAttttt               >  1:708853/1‑148 (MQ=255)
                                                                                                                                    aaGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACAACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAttttt               >  1:1003299/1‑148 (MQ=255)
                                                                                                                                     aGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTc              <  2:1894595/148‑1 (MQ=255)
                                                                                                                                      gTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCa             >  2:2598992/1‑148 (MQ=255)
                                                                                                                                              aaCTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAgg     >  1:3257132/1‑148 (MQ=255)
                                                                                                                                              aaCTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAgg     <  2:938501/148‑1 (MQ=255)
                                                                                                                                               aCTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAAAGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTAACACAAATGGTGTACTGGTAACAGACACAGCAAATGTGTCTATTTTTCATGTAAATGt    >  2:1291252/1‑148 (MQ=255)
                                                                                                                                                ctttttTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTa   <  2:3134503/148‑1 (MQ=255)
                                                                                                                                                 ttcttTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACAACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTaa  <  1:1391589/148‑1 (MQ=255)
                                                                                                                                                 ttcttTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACAACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTaa  <  1:3166083/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAA  >  CP009273/652450‑652742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTT  >  CP009273/652448‑652746
                                                                                                                                                     |                                                                                                                                                     
CCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAACTTT                                                                                                                                                       >  SRR19973066.1908615/1‑150 (MQ=60)
  TCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTT                                                                                                                                                     >  SRR19973066.1102605/1‑150 (MQ=60)
  TCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTT                                                                                                                                                     >  SRR19973066.1103805/1‑150 (MQ=60)
    TCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTA                                                                                                                                                   >  SRR19973066.982620/1‑150 (MQ=60)
    TCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTA                                                                                                                                                   >  SRR19973066.982592/1‑150 (MQ=60)
    TCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTA                                                                                                                                                   >  SRR19973066.982424/1‑150 (MQ=60)
      CCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAA                                                                                                                                                 <  SRR19973066.805485/150‑1 (MQ=60)
      CCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAA                                                                                                                                                 <  SRR19973066.805538/150‑1 (MQ=60)
      CCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAA                                                                                                                                                 <  SRR19973066.1907201/150‑1 (MQ=60)
        GGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAAT                                                                                                                                               <  SRR19973066.1736711/150‑1 (MQ=60)
        GGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAAT                                                                                                                                               <  SRR19973066.1051580/150‑1 (MQ=60)
        GGTTCTACTGCCATTGGACTCCGTGGGTTATGTCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAAT                                                                                                                                               <  SRR19973066.1051584/150‑1 (MQ=60)
              ACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAG                                                                                                                                         >  SRR19973066.172124/1‑150 (MQ=60)
              ACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAG                                                                                                                                         <  SRR19973066.3240652/150‑1 (MQ=60)
              ACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAG                                                                                                                                         <  SRR19973066.3240806/150‑1 (MQ=60)
              ACTGCCAGTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAG                                                                                                                                         >  SRR19973066.172156/1‑150 (MQ=60)
                 GCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTA                                                                                                                                      <  SRR19973066.1952793/150‑1 (MQ=60)
                   CATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAA                                                                                                                                    >  SRR19973066.2731871/1‑150 (MQ=60)
                   CATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAA                                                                                                                                    >  SRR19973066.3302996/1‑150 (MQ=60)
                    ATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAA                                                                                                                                   >  SRR19973066.3080021/1‑150 (MQ=60)
                     TTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAAT                                                                                                                                  <  SRR19973066.2497815/150‑1 (MQ=60)
                      TGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATA                                                                                                                                 >  SRR19973066.2739231/1‑150 (MQ=60)
                        GCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAG                                                                                                                               <  SRR19973066.3246645/150‑1 (MQ=60)
                          CTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTA                                                                                                                             >  SRR19973066.1320013/1‑150 (MQ=60)
                          CTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTA                                                                                                                             >  SRR19973066.1320010/1‑150 (MQ=60)
                            CCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAAC                                                                                                                           <  SRR19973066.2866524/150‑1 (MQ=60)
                            CCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAAC                                                                                                                           <  SRR19973066.1778173/150‑1 (MQ=60)
                              GTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACAT                                                                                                                         >  SRR19973066.2590272/1‑150 (MQ=60)
                              GTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACAT                                                                                                                         >  SRR19973066.316915/1‑150 (MQ=60)
                              GTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACAT                                                                                                                         >  SRR19973066.2931448/1‑150 (MQ=60)
                                GGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCA                                                                                                                       <  SRR19973066.2391343/150‑1 (MQ=60)
                                  GTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAA                                                                                                                     >  SRR19973066.867449/1‑150 (MQ=60)
                                    TATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGATACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAA                                                                                                                   >  SRR19973066.2319449/1‑150 (MQ=60)
                                    TATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAA                                                                                                                   <  SRR19973066.2795286/150‑1 (MQ=60)
                                     ATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAAT                                                                                                                  <  SRR19973066.3120989/150‑1 (MQ=60)
                                     ATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAAT                                                                                                                  <  SRR19973066.547449/150‑1 (MQ=60)
                                       GGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAA                                                                                                                >  SRR19973066.1735122/1‑150 (MQ=60)
                                       GGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAA                                                                                                                >  SRR19973066.814879/1‑150 (MQ=60)
                                        GCCCTGTGACTTTTCAGATGACCTACATTCCGTGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAAC                                                                                                               <  SRR19973066.1172493/150‑1 (MQ=60)
                                        GCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAAC                                                                                                               <  SRR19973066.1817391/150‑1 (MQ=60)
                                        GCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAAC                                                                                                               <  SRR19973066.2808427/150‑1 (MQ=60)
                                        GCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAAC                                                                                                               <  SRR19973066.1016794/150‑1 (MQ=60)
                                         CCCAGTGACTTTTCAGATGACCTACATTCCGTGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATATCTAAAATAAGTAACATCAAAAATAACG                                                                                                              >  SRR19973066.2353124/1‑150 (MQ=60)
                                         CCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATCCCAATGTGTACTTTGCCTGGATGCGCGTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACG                                                                                                              <  SRR19973066.3291043/150‑1 (MQ=60)
                                           CAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCG                                                                                                            <  SRR19973066.317373/150‑1 (MQ=60)
                                           CAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCG                                                                                                            <  SRR19973066.2627540/150‑1 (MQ=60)
                                           CAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCG                                                                                                            <  SRR19973066.2627523/150‑1 (MQ=60)
                                           CAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCG                                                                                                            <  SRR19973066.894457/150‑1 (MQ=60)
                                               GACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTT                                                                                                        >  SRR19973066.1946568/1‑150 (MQ=60)
                                                  TTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTA                                                                                                     <  SRR19973066.1649668/150‑1 (MQ=60)
                                                      CAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAATTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCAC                                                                                                 <  SRR19973066.994299/150‑1 (MQ=60)
                                                      CAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCAC                                                                                                 <  SRR19973066.2589306/150‑1 (MQ=60)
                                                      CAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCAC                                                                                                 <  SRR19973066.2589998/150‑1 (MQ=60)
                                                           GACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTT                                                                                            >  SRR19973066.241591/1‑150 (MQ=60)
                                                           GACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTT                                                                                            <  SRR19973066.2456603/150‑1 (MQ=60)
                                                           GACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTT                                                                                            <  SRR19973066.2456779/150‑1 (MQ=60)
                                                           GACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTT                                                                                            >  SRR19973066.1837518/1‑150 (MQ=60)
                                                           GACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTT                                                                                            >  SRR19973066.1837723/1‑150 (MQ=60)
                                                             CCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAG                                                                                          >  SRR19973066.2889828/1‑150 (MQ=60)
                                                               TACATTCCGTGTAACTACAAGAATTGCAATGTGTAGTTTGCCTGGATGCGCTTTCTGTTTTGATACAAATGAATTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAAAATCAAAAATAACGCGACTTTTATCACTTTTTAGTA                                                                                        <  SRR19973066.2239301/150‑1 (MQ=60)
                                                               TACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTA                                                                                        <  SRR19973066.2239307/150‑1 (MQ=60)
                                                               TACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTA                                                                                        >  SRR19973066.3265257/1‑150 (MQ=60)
                                                               TACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTA                                                                                        >  SRR19973066.300234/1‑150 (MQ=60)
                                                               TACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTA                                                                                        >  SRR19973066.945330/1‑150 (MQ=60)
                                                               TACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTA                                                                                        >  SRR19973066.947280/1‑150 (MQ=60)
                                                                  ATTCCGGGTACCTACAACAATGTCTATGTGTACTTTGCCTGGATGCGCTTTAAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAG                                                                                     <  SRR19973066.2558189/150‑1 (MQ=60)
                                                                  AGTCCGGGTACCTACAACACTGGCTATGTGTACTTTGCCTGGATGCGCTTTAAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAG                                                                                     <  SRR19973066.1265160/150‑1 (MQ=60)
                                                                   TTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGT                                                                                    <  SRR19973066.398994/150‑1 (MQ=60)
                                                                      CGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTAC                                                                                 >  SRR19973066.1562785/1‑150 (MQ=60)
                                                                        GTTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTGTTATCACTTTTTAGTAAAGTTACAC                                                                               >  SRR19973066.1856760/1‑150 (MQ=60)
                                                                        GGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACAC                                                                               >  SRR19973066.1858517/1‑150 (MQ=60)
                                                                            CCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGA                                                                           >  SRR19973066.2146605/1‑150 (MQ=60)
                                                                                 AACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAG                                                                      >  SRR19973066.1384303/1‑150 (MQ=60)
                                                                                 AACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAG                                                                      >  SRR19973066.3236151/1‑150 (MQ=60)
                                                                                 AACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAG                                                                      <  SRR19973066.306240/150‑1 (MQ=60)
                                                                                 AACAAGGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAG                                                                      <  SRR19973066.305917/150‑1 (MQ=60)
                                                                                    AATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGT                                                                   <  SRR19973066.2093697/150‑1 (MQ=60)
                                                                                    AATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGT                                                                   <  SRR19973066.3089821/150‑1 (MQ=60)
                                                                                     ATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTA                                                                  >  SRR19973066.311523/1‑150 (MQ=60)
                                                                                       GGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACC                                                                <  SRR19973066.471030/150‑1 (MQ=60)
                                                                                        GCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCA                                                               >  SRR19973066.3200487/1‑150 (MQ=60)
                                                                                        GCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCA                                                               >  SRR19973066.3200447/1‑150 (MQ=60)
                                                                                        GCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCA                                                               >  SRR19973066.1414901/1‑150 (MQ=60)
                                                                                          AATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACA                                                             >  SRR19973066.3277462/1‑150 (MQ=60)
                                                                                          AATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACA                                                             >  SRR19973066.2174111/1‑150 (MQ=60)
                                                                                             GTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATT                                                          <  SRR19973066.1466525/150‑1 (MQ=60)
                                                                                              TGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTG                                                         <  SRR19973066.1808357/150‑1 (MQ=60)
                                                                                               GTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGG                                                        <  SRR19973066.1182171/150‑1 (MQ=60)
                                                                                               GTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGG                                                        <  SRR19973066.1417486/150‑1 (MQ=60)
                                                                                               GTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGG                                                        <  SRR19973066.1417480/150‑1 (MQ=60)
                                                                                                 ACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTG                                                      <  SRR19973066.1801577/150‑1 (MQ=60)
                                                                                                       CCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGG                                                >  SRR19973066.1640004/1‑150 (MQ=60)
                                                                                                       CCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGG                                                >  SRR19973066.429991/1‑150 (MQ=60)
                                                                                                              GCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGA                                         <  SRR19973066.2334943/150‑1 (MQ=60)
                                                                                                               CGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGAC                                        <  SRR19973066.1262532/150‑1 (MQ=60)
                                                                                                                 CTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACAC                                      >  SRR19973066.3101719/1‑150 (MQ=60)
                                                                                                                      AGTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCAT                                 >  SRR19973066.503338/1‑150 (MQ=60)
                                                                                                                      AGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACAACAATTGGTGTACTGGTAACCGACACAGCAT                                 <  SRR19973066.1373818/150‑1 (MQ=60)
                                                                                                                       GTTTTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATT                                <  SRR19973066.2651435/150‑1 (MQ=60)
                                                                                                                          TTGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGT                             <  SRR19973066.2977068/150‑1 (MQ=60)
                                                                                                                           TGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTG                            <  SRR19973066.1200731/150‑1 (MQ=60)
                                                                                                                           TGAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTG                            <  SRR19973066.2510404/150‑1 (MQ=60)
                                                                                                                            GAGACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGT                           >  SRR19973066.1813617/1‑150 (MQ=60)
                                                                                                                             AGACAAATTAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTC                          <  SRR19973066.1833403/150‑1 (MQ=60)
                                                                                                                               ACAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTA                        >  SRR19973066.1277727/1‑150 (MQ=60)
                                                                                                                                CAAATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTAT                       >  SRR19973066.3193706/1‑150 (MQ=60)
                                                                                                                                  AATGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTT                     <  SRR19973066.2599966/150‑1 (MQ=60)
                                                                                                                                    TGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTT                   >  SRR19973066.714133/1‑150 (MQ=60)
                                                                                                                                    TGAAGTTTTAGTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTT                   >  SRR19973066.714098/1‑150 (MQ=60)
                                                                                                                                    TGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACAACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTT                   >  SRR19973066.1010690/1‑150 (MQ=60)
                                                                                                                                              GTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGG         <  SRR19973066.3429872/150‑1 (MQ=60)
                                                                                                                                              GTAACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGG         >  SRR19973066.3278563/1‑150 (MQ=60)
                                                                                                                                                 ACTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAA      >  SRR19973066.2068540/1‑150 (MQ=60)
                                                                                                                                                  CTTTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAAT     >  SRR19973066.2170635/1‑150 (MQ=60)
                                                                                                                                                   TTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACAACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATT    <  SRR19973066.1401846/150‑1 (MQ=60)
                                                                                                                                                   TTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACAACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATT    <  SRR19973066.1401627/150‑1 (MQ=60)
                                                                                                                                                   TTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACAACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATT    <  SRR19973066.3187173/150‑1 (MQ=60)
                                                                                                                                                    TTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTT   <  SRR19973066.941409/150‑1 (MQ=60)
                                                                                                                                                    TTTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTT   <  SRR19973066.2461132/150‑1 (MQ=60)
                                                                                                                                                     TTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTT  <  SRR19973066.2971216/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAAGTTTTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTT  >  CP009273/652448‑652746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑