Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 888,964 C→T 100% intergenic (+75/‑276) ybjN → / → potF negative regulator of motility; multicopy suppressor of coaA(Ts)/putrescine transporter subunit: periplasmic‑binding component of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273888,9640CT97.4% 757.6 / 7.5 233intergenic (+75/‑276)ybjN/potFnegative regulator of motility; multicopy suppressor of coaA(Ts)/putrescine transporter subunit: periplasmic‑binding component of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base C (3/3);  new base T (99/127);  total (102/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 238 total aligned reads displayed.

CCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAG  >  CP009273/888820‑889108
                                                                                                                                                |                                                                                                                                                
ccATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTa                                                                                                                                               <  1:2198863/148‑1 (MQ=255)
ccATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTa                                                                                                                                               <  2:628335/148‑1 (MQ=255)
  aTCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTaaa                                                                                                                                             >  2:1790373/1‑148 (MQ=255)
    cAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGa                                                                                                                                           <  1:1763196/148‑1 (MQ=255)
    cAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGa                                                                                                                                           <  1:1298052/148‑1 (MQ=255)
    cAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGa                                                                                                                                           <  2:448748/148‑1 (MQ=255)
     aaCTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGat                                                                                                                                          <  2:3528306/148‑1 (MQ=255)
      aCTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGatt                                                                                                                                         <  2:2792336/148‑1 (MQ=255)
      aCTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGatt                                                                                                                                         <  2:813275/148‑1 (MQ=255)
       ctgctgTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGatta                                                                                                                                        <  1:1631009/148‑1 (MQ=255)
       ctgctgTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGatta                                                                                                                                        <  1:3061231/148‑1 (MQ=255)
          ctgTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTAttt                                                                                                                                     <  1:1790373/148‑1 (MQ=255)
           tgTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTc                                                                                                                                    >  2:1509540/1‑148 (MQ=255)
           tgTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTc                                                                                                                                    <  1:502903/148‑1 (MQ=255)
            gTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCa                                                                                                                                   >  1:2979980/1‑148 (MQ=255)
                  gACTGATGATGAAGGGCAACACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGGGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTtctc                                                                                                                             <  2:3088221/148‑1 (MQ=255)
                  gACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTtctc                                                                                                                             <  2:634802/148‑1 (MQ=255)
                    cTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCtt                                                                                                                           <  1:125229/148‑1 (MQ=255)
                     tgatgatgaAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTtg                                                                                                                          <  2:1769704/148‑1 (MQ=255)
                     ggatgatgaAGGGCAAAACAACGTTACCGAAAACTCTTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTtg                                                                                                                          <  2:2240490/147‑1 (MQ=255)
                        tgatgaAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTGTCTTgtgt                                                                                                                       >  1:3375105/1‑148 (MQ=255)
                        tgatgaAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTgtgt                                                                                                                       >  1:3375101/1‑148 (MQ=255)
                             aaGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGAtt                                                                                                                  >  2:772026/1‑148 (MQ=255)
                             aaGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGAtt                                                                                                                  <  2:460564/148‑1 (MQ=255)
                              aGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGAttt                                                                                                                 <  1:2111208/148‑1 (MQ=255)
                                  cAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCt                                                                                                             <  1:1384151/148‑1 (MQ=255)
                                     aacaacGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTa                                                                                                          >  1:1260177/1‑148 (MQ=255)
                                      acaacGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTAt                                                                                                         <  1:1936407/148‑1 (MQ=255)
                                       caacGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATc                                                                                                        >  2:668998/1‑148 (MQ=255)
                                       caacGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATc                                                                                                        >  2:2494913/1‑148 (MQ=255)
                                          cGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATcaca                                                                                                     >  1:2034120/1‑148 (MQ=255)
                                          cGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATcaca                                                                                                     <  1:1890789/148‑1 (MQ=255)
                                          cGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATcaca                                                                                                     <  1:904769/148‑1 (MQ=255)
                                             tACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATag                                                                                                  <  1:88737/148‑1 (MQ=255)
                                             tACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATag                                                                                                  >  2:3129093/1‑148 (MQ=255)
                                             tACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATag                                                                                                  <  2:2157429/148‑1 (MQ=255)
                                                 gAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCa                                                                                              <  1:1689764/148‑1 (MQ=255)
                                                  aaaaCTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCaa                                                                                             >  2:1579532/1‑148 (MQ=255)
                                                  aaaaCTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCaa                                                                                             >  2:2855841/1‑148 (MQ=255)
                                                   aaaCTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCaaa                                                                                            <  1:2829057/148‑1 (MQ=255)
                                                   aaaCTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCaaa                                                                                            >  2:2622957/1‑148 (MQ=255)
                                                     aCTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCACAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCCAata                                                                                          >  2:2572144/1‑148 (MQ=255)
                                                        aTTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGc                                                                                       <  2:2282044/148‑1 (MQ=255)
                                                        aTTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGc                                                                                       >  2:2999378/1‑148 (MQ=255)
                                                          ttCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCAt                                                                                     >  2:2252018/1‑148 (MQ=255)
                                                          ttCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCAt                                                                                     >  1:1003267/1‑148 (MQ=255)
                                                            cctccACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATaa                                                                                   <  2:1003267/148‑1 (MQ=255)
                                                              tccACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATaaaa                                                                                 <  1:2305409/148‑1 (MQ=255)
                                                                    gATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTGTCTTGGGTCGATTTGGCTTTATCACATATAGCAAATATGCATAAAAATTTGt                                                                           >  1:2533957/1‑148 (MQ=255)
                                                                    gATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGt                                                                           <  1:2862221/148‑1 (MQ=255)
                                                                     aTAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGtt                                                                          <  1:239033/148‑1 (MQ=255)
                                                                     aTAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGtt                                                                          >  1:3554721/1‑148 (MQ=255)
                                                                         cTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAGTTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAAt                                                                      <  1:1408755/148‑1 (MQ=255)
                                                                             tttCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCg                                                                  <  1:1809253/148‑1 (MQ=255)
                                                                             tttCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCGTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCg                                                                  >  1:2456254/1‑148 (MQ=255)
                                                                              ttCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAACTTTGTTAAATACCGt                                                                 >  2:1427977/1‑148 (MQ=255)
                                                                              ttCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGt                                                                 <  2:897738/148‑1 (MQ=255)
                                                                              ttCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGt                                                                 <  2:2979980/148‑1 (MQ=255)
                                                                              ttCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGt                                                                 <  1:1165973/148‑1 (MQ=255)
                                                                               tCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGtt                                                                <  1:1753975/148‑1 (MQ=255)
                                                                               tCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATTCATAAAAATTTGTTAAATACCttt                                                                >  1:992845/1‑148 (MQ=255)
                                                                                    cACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTTTCACATAGAGCAAATATGCATTAAAATTTGTTAAATACCGTTTTTTa                                                           >  2:1321686/1‑148 (MQ=255)
                                                                                    cACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTa                                                           >  2:1228112/1‑148 (MQ=255)
                                                                                      cGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAAt                                                         <  2:1658192/148‑1 (MQ=255)
                                                                                       gCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATc                                                        <  1:1083570/148‑1 (MQ=255)
                                                                                       gCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCTTTTTTTAATc                                                        <  2:1985733/148‑1 (MQ=255)
                                                                                               tGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCtat                                                <  2:1527248/148‑1 (MQ=255)
                                                                                               tGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCtat                                                <  2:3274933/148‑1 (MQ=255)
                                                                                                 gTGCAGTGGCTGCGCGCTTCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAg                                              <  2:2725516/148‑1 (MQ=255)
                                                                                                      gTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCa                                         >  2:3496627/1‑148 (MQ=255)
                                                                                                      gTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTT‑TTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCaa                                        <  2:520038/148‑1 (MQ=255)
                                                                                                       tGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCCAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCaa                                        >  2:721315/1‑148 (MQ=255)
                                                                                                        ggCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCaaa                                       >  1:3044937/1‑148 (MQ=255)
                                                                                                        ggCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCaaa                                       >  1:3045189/1‑148 (MQ=255)
                                                                                                          cTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAAcc                                     >  1:165695/1‑148 (MQ=255)
                                                                                                          cTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAAcc                                     <  1:316320/148‑1 (MQ=255)
                                                                                                               cgcTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGc                                <  1:1727324/148‑1 (MQ=255)
                                                                                                                gcTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCt                               <  1:3181825/148‑1 (MQ=255)
                                                                                                                     aaaaTTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACACTGGCTAAAGt                          >  2:3430708/1‑148 (MQ=255)
                                                                                                                      aaaTTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAATCCCTGGCTAAAGtt                         >  2:3135757/1‑148 (MQ=255)
                                                                                                                       aaTTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTCAAGTTa                        >  2:2207633/1‑148 (MQ=255)
                                                                                                                       aaTTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTa                        >  2:1921740/1‑148 (MQ=255)
                                                                                                                       aaTTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTa                        <  1:816187/148‑1 (MQ=255)
                                                                                                                       aaTTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCAGAGCTATAGTCTCAAACCCTGGCTAAAGTTa                        >  1:2774669/1‑148 (MQ=255)
                                                                                                                        aTTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTAt                       >  1:3281508/1‑148 (MQ=255)
                                                                                                                        aTTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTAt                       <  1:2999813/148‑1 (MQ=255)
                                                                                                                         ttATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTAtt                      >  2:276230/1‑148 (MQ=255)
                                                                                                                         ttATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTAtt                      >  2:1234618/1‑148 (MQ=255)
                                                                                                                          tATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTc                     >  2:1726527/1‑148 (MQ=255)
                                                                                                                              tgctgTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGc                 >  1:2463142/1‑148 (MQ=255)
                                                                                                                              tgctgTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGc                 >  2:1558980/1‑148 (MQ=255)
                                                                                                                                   tttttAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGc            >  1:663694/1‑148 (MQ=255)
                                                                                                                                    ttttAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCt           >  1:1994708/1‑148 (MQ=255)
                                                                                                                                     tttAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCACATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCtt          >  2:1128166/1‑148 (MQ=255)
                                                                                                                                        aaCCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTta       <  1:69672/148‑1 (MQ=255)
                                                                                                                                           cTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTtatat    >  2:2548780/1‑148 (MQ=255)
                                                                                                                                           cTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTtatat    >  2:3349961/1‑148 (MQ=255)
                                                                                                                                           cTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTtatat    >  2:2868101/1‑148 (MQ=255)
                                                                                                                                            ttttttTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTtatata   <  2:357153/148‑1 (MQ=255)
                                                                                                                                             ttttttAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAg  >  1:406398/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAG  >  CP009273/888820‑889108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATGCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAG  >  CP009273/888815‑889112
                                                                                                                                                     |                                                                                                                                                    
AATGCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGATCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTT                                                                                                                                                      <  SRR19973067.1533462/150‑1 (MQ=60)
 ATGCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTT                                                                                                                                                     >  SRR19973067.523553/1‑150 (MQ=60)
  TGCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTT                                                                                                                                                    <  SRR19973067.1352428/150‑1 (MQ=60)
  TGCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTT                                                                                                                                                    <  SRR19973067.2751939/150‑1 (MQ=60)
  TGCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTT                                                                                                                                                    <  SRR19973067.2751904/150‑1 (MQ=60)
  TGCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTT                                                                                                                                                    <  SRR19973067.1352246/150‑1 (MQ=60)
     CCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAA                                                                                                                                                 >  SRR19973067.1293993/1‑150 (MQ=60)
     CCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAA                                                                                                                                                 <  SRR19973067.2213533/150‑1 (MQ=60)
        TCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGAT                                                                                                                                              <  SRR19973067.2033901/150‑1 (MQ=60)
        TCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGAT                                                                                                                                              <  SRR19973067.1697001/150‑1 (MQ=60)
        TCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGAT                                                                                                                                              <  SRR19973067.226780/150‑1 (MQ=60)
         CAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATT                                                                                                                                             <  SRR19973067.1775190/150‑1 (MQ=60)
         CAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATT                                                                                                                                             <  SRR19973067.1306915/150‑1 (MQ=60)
         CAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATT                                                                                                                                             <  SRR19973067.1306919/150‑1 (MQ=60)
            CTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATT                                                                                                                                          <  SRR19973067.3080720/150‑1 (MQ=60)
            CTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATT                                                                                                                                          <  SRR19973067.1642233/150‑1 (MQ=60)
              GCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTC                                                                                                                                        <  SRR19973067.870127/150‑1 (MQ=60)
               CTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCA                                                                                                                                       >  SRR19973067.2998801/1‑150 (MQ=60)
               CTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCA                                                                                                                                       <  SRR19973067.1802438/150‑1 (MQ=60)
               CTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCA                                                                                                                                       <  SRR19973067.1802453/150‑1 (MQ=60)
                TGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCAC                                                                                                                                      <  SRR19973067.1061469/150‑1 (MQ=60)
                TGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCAC                                                                                                                                      <  SRR19973067.506349/150‑1 (MQ=60)
                    ACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCT                                                                                                                                  >  SRR19973067.1644121/1‑150 (MQ=60)
                    ACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCT                                                                                                                                  <  SRR19973067.3327309/150‑1 (MQ=60)
                       GACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTT                                                                                                                               >  SRR19973067.1784617/1‑150 (MQ=60)
                       GACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTT                                                                                                                               <  SRR19973067.1782811/150‑1 (MQ=60)
                        ACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTG                                                                                                                              >  SRR19973067.2049919/1‑150 (MQ=60)
                         CTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGT                                                                                                                             <  SRR19973067.126387/150‑1 (MQ=60)
                         CTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGT                                                                                                                             <  SRR19973067.126375/150‑1 (MQ=60)
                         CTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTGTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGT                                                                                                                             <  SRR19973067.1843947/150‑1 (MQ=60)
                           GATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTGTCTTGTGT                                                                                                                           >  SRR19973067.3396558/1‑150 (MQ=60)
                           GATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGT                                                                                                                           >  SRR19973067.3396555/1‑150 (MQ=60)
                           GATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGT                                                                                                                           >  SRR19973067.3396554/1‑150 (MQ=60)
                              GATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTTAAGATTATTTCACTTCTCTTGTGTCGA                                                                                                                        >  SRR19973067.399873/1‑150 (MQ=60)
                                   AGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGG                                                                                                                   <  SRR19973067.2125248/150‑1 (MQ=60)
                                      GCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTT                                                                                                                <  SRR19973067.3198404/150‑1 (MQ=60)
                                       CAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTT                                                                                                               <  SRR19973067.1393574/150‑1 (MQ=60)
                                        AAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTA                                                                                                              >  SRR19973067.2198271/1‑150 (MQ=60)
                                        AAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTA                                                                                                              >  SRR19973067.1268719/1‑150 (MQ=60)
                                        AAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTA                                                                                                              >  SRR19973067.1999167/1‑150 (MQ=60)
                                         AAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTAT                                                                                                             <  SRR19973067.524314/150‑1 (MQ=60)
                                         AAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTAT                                                                                                             >  SRR19973067.2267469/1‑150 (MQ=60)
                                          AACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATC                                                                                                            <  SRR19973067.783066/150‑1 (MQ=60)
                                          AACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATC                                                                                                            <  SRR19973067.783054/150‑1 (MQ=60)
                                           ACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCA                                                                                                           <  SRR19973067.1948960/150‑1 (MQ=60)
                                           ACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCA                                                                                                           <  SRR19973067.1949614/150‑1 (MQ=60)
                                             AACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACA                                                                                                         >  SRR19973067.2047746/1‑150 (MQ=60)
                                               CGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATA                                                                                                       <  SRR19973067.910743/150‑1 (MQ=60)
                                               CGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATA                                                                                                       <  SRR19973067.1903692/150‑1 (MQ=60)
                                               CGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATA                                                                                                       <  SRR19973067.910561/150‑1 (MQ=60)
                                                  TACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAG                                                                                                    <  SRR19973067.1733453/150‑1 (MQ=60)
                                                  TACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAG                                                                                                    <  SRR19973067.89688/150‑1 (MQ=60)
                                                  TACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAG                                                                                                    <  SRR19973067.1733441/150‑1 (MQ=60)
                                                     CGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAA                                                                                                 >  SRR19973067.2796440/1‑150 (MQ=60)
                                                     CGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAA                                                                                                 >  SRR19973067.970745/1‑150 (MQ=60)
                                                      GAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAA                                                                                                <  SRR19973067.1701397/150‑1 (MQ=60)
                                                       AAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAAT                                                                                               <  SRR19973067.2017589/150‑1 (MQ=60)
                                                        AAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATA                                                                                              <  SRR19973067.2847245/150‑1 (MQ=60)
                                                        AAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATA                                                                                              <  SRR19973067.3029544/150‑1 (MQ=60)
                                                        AAACTATGTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATA                                                                                              <  SRR19973067.2847274/150‑1 (MQ=60)
                                                           CTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGC                                                                                           >  SRR19973067.2396264/1‑150 (MQ=60)
                                                             ATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATTCAT                                                                                         >  SRR19973067.1010160/1‑150 (MQ=60)
                                                             ATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCAT                                                                                         <  SRR19973067.3023137/150‑1 (MQ=60)
                                                             ATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCAT                                                                                         <  SRR19973067.3084162/150‑1 (MQ=60)
                                                                   TCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAAT                                                                                   <  SRR19973067.348123/150‑1 (MQ=60)
                                                                   TCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAAT                                                                                   <  SRR19973067.2320598/150‑1 (MQ=60)
                                                                    CCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATT                                                                                  <  SRR19973067.3535053/150‑1 (MQ=60)
                                                                     CACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTT                                                                                 <  SRR19973067.2854915/150‑1 (MQ=60)
                                                                      ACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTG                                                                                <  SRR19973067.938779/150‑1 (MQ=60)
                                                                      ACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTG                                                                                <  SRR19973067.2600810/150‑1 (MQ=60)
                                                                      ACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTG                                                                                <  SRR19973067.3237868/150‑1 (MQ=60)
                                                                       CTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTGTCTTGGGTCGATTTGGCTTTATCACATATAGCAAATATGCATAAAAATTTGT                                                                               >  SRR19973067.2550560/1‑150 (MQ=60)
                                                                        TGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTT                                                                              >  SRR19973067.3577127/1‑150 (MQ=60)
                                                                         GATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTA                                                                             <  SRR19973067.2880564/150‑1 (MQ=60)
                                                                          ATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAA                                                                            <  SRR19973067.240706/150‑1 (MQ=60)
                                                                           TAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGGTTTTAAACTTTTCTTAAAGATTATTTGACGTCTCTGTGGTGGAGTTGGCTTTATCACATAGAGCAATTATGGAAAAAAATTTGTTAAA                                                                           >  SRR19973067.2403177/1‑150 (MQ=60)
                                                                             ACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATAACATATAGCAAATATGCATAAAAATTTGTTAAATA                                                                         >  SRR19973067.1467806/1‑150 (MQ=60)
                                                                              CTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAGTTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATAC                                                                        <  SRR19973067.1418297/150‑1 (MQ=60)
                                                                              CTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATAC                                                                        <  SRR19973067.1416529/150‑1 (MQ=60)
                                                                                CCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCG                                                                      >  SRR19973067.2472394/1‑150 (MQ=60)
                                                                                CCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGGGTCGATTTTGCTTTATCACATAGAGCAAATATTCATAAAAATTTGTTAAATACCG                                                                      >  SRR19973067.2472393/1‑150 (MQ=60)
                                                                                CCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCGTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCG                                                                      >  SRR19973067.2472403/1‑150 (MQ=60)
                                                                                  TTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTT                                                                    <  SRR19973067.1821481/150‑1 (MQ=60)
                                                                                  TTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATTCATAAAAATTTGTTAAATACCTTT                                                                    >  SRR19973067.999679/1‑150 (MQ=60)
                                                                                  TTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCTTT                                                                    >  SRR19973067.1003151/1‑150 (MQ=60)
                                                                                   TTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTT                                                                   <  SRR19973067.1173720/150‑1 (MQ=60)
                                                                                   TTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTT                                                                   <  SRR19973067.2957688/150‑1 (MQ=60)
                                                                                    TCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTT                                                                  <  SRR19973067.1765897/150‑1 (MQ=60)
                                                                                    TCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTT                                                                  <  SRR19973067.1765924/150‑1 (MQ=60)
                                                                                      GAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATTACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTT                                                                <  SRR19973067.3117115/150‑1 (MQ=60)
                                                                                            GCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCG                                                          <  SRR19973067.1090984/150‑1 (MQ=60)
                                                                                              AGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAG                                                        <  SRR19973067.1231539/150‑1 (MQ=60)
                                                                                                           GTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTCTAGTCTCAAA                                           >  SRR19973067.3064359/1‑150 (MQ=60)
                                                                                                           GTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAA                                           >  SRR19973067.3064334/1‑150 (MQ=60)
                                                                                                           GTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAA                                           >  SRR19973067.3064587/1‑150 (MQ=60)
                                                                                                             GGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACC                                         >  SRR19973067.167051/1‑150 (MQ=60)
                                                                                                              GCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTTTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCC                                        >  SRR19973067.3072138/1‑150 (MQ=60)
                                                                                                               CTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCT                                       <  SRR19973067.318443/150‑1 (MQ=60)
                                                                                                               CTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCT                                       <  SRR19973067.634822/150‑1 (MQ=60)
                                                                                                                    CGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTA                                  <  SRR19973067.1739156/150‑1 (MQ=60)
                                                                                                                     GCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAA                                 <  SRR19973067.3201954/150‑1 (MQ=60)
                                                                                                                          AAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCAGAGCTATAGTCTCAAACCCTGGCTAAAGTTA                            >  SRR19973067.2792592/1‑150 (MQ=60)
                                                                                                                           AAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTAT                           >  SRR19973067.248257/1‑150 (MQ=60)
                                                                                                                           AAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTAT                           >  SRR19973067.3302086/1‑150 (MQ=60)
                                                                                                                            AATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATT                          <  SRR19973067.1771553/150‑1 (MQ=60)
                                                                                                                            AATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATT                          <  SRR19973067.821782/150‑1 (MQ=60)
                                                                                                                             ATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTC                         <  SRR19973067.3018854/150‑1 (MQ=60)
                                                                                                                             ATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTC                         <  SRR19973067.3018410/150‑1 (MQ=60)
                                                                                                                                 TCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGC                     >  SRR19973067.2479317/1‑150 (MQ=60)
                                                                                                                                 TCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGC                     >  SRR19973067.2479331/1‑150 (MQ=60)
                                                                                                                                     CTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATG                 >  SRR19973067.1268522/1‑150 (MQ=60)
                                                                                                                                      TGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGC                >  SRR19973067.1831119/1‑150 (MQ=60)
                                                                                                                                      TGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGC                >  SRR19973067.668226/1‑150 (MQ=60)
                                                                                                                                       GTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGGTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGGTGCT               >  SRR19973067.2554794/1‑150 (MQ=60)
                                                                                                                                       GTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCT               >  SRR19973067.2008179/1‑150 (MQ=60)
                                                                                                                                         TTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTTGCTTTATCACATAGAGCAAATATGAATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTCTAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTT             >  SRR19973067.1365431/1‑150 (MQ=60)
                                                                                                                                          TTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTT            >  SRR19973067.1483139/1‑150 (MQ=60)
                                                                                                                                          TTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTT            >  SRR19973067.3256106/1‑150 (MQ=60)
                                                                                                                                             AACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATA         <  SRR19973067.70483/150‑1 (MQ=60)
                                                                                                                                             AACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATA         <  SRR19973067.70487/150‑1 (MQ=60)
                                                                                                                                                CTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAG      >  SRR19973067.409814/1‑150 (MQ=60)
                                                                                                                                                CTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAG      >  SRR19973067.409226/1‑150 (MQ=60)
                                                                                                                                                 TTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGC     <  SRR19973067.1147583/150‑1 (MQ=60)
                                                                                                                                                   TTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGA   <  SRR19973067.425127/150‑1 (MQ=60)
                                                                                                                                                   TTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGA   >  SRR19973067.3264939/1‑150 (MQ=60)
                                                                                                                                                    TTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAG  <  SRR19973067.2639380/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
AATGCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAG  >  CP009273/888815‑889112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑