Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 888,964 C→T 100% intergenic (+75/‑276) ybjN → / → potF negative regulator of motility; multicopy suppressor of coaA(Ts)/putrescine transporter subunit: periplasmic‑binding component of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273888,9640CT97.5% 800.8 / 6.3 244intergenic (+75/‑276)ybjN/potFnegative regulator of motility; multicopy suppressor of coaA(Ts)/putrescine transporter subunit: periplasmic‑binding component of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base C (4/2);  new base T (127/110);  total (132/112)
Fisher's exact test for biased strand distribution p-value = 6.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 246 total aligned reads displayed.

GCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCG  >  CP009273/888818‑889110
                                                                                                                                                  |                                                                                                                                                  
gCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTa                                                                                                                                                 <  2:4605395/150‑1 (MQ=255)
 cccATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTaa                                                                                                                                                >  2:4485289/1‑150 (MQ=255)
    aTCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGa                                                                                                                                             >  1:1081371/1‑150 (MQ=255)
    aTCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGa                                                                                                                                             >  2:1412455/1‑150 (MQ=255)
    aTCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGa                                                                                                                                             >  2:2486151/1‑150 (MQ=255)
      cAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGatt                                                                                                                                           >  1:3077267/1‑150 (MQ=255)
          tgctgTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTAttt                                                                                                                                       >  1:250116/1‑150 (MQ=255)
            ctgTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCa                                                                                                                                     <  1:4879962/150‑1 (MQ=255)
              gTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACt                                                                                                                                   <  2:160521/150‑1 (MQ=255)
               ttACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTCTCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACtt                                                                                                                                  <  1:3940419/150‑1 (MQ=255)
               ttACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACtt                                                                                                                                  <  1:46443/150‑1 (MQ=255)
                tACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTtc                                                                                                                                 >  2:4528349/1‑150 (MQ=255)
                 aCCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTtct                                                                                                                                <  1:3483003/150‑1 (MQ=255)
                     aCTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTtg                                                                                                                            <  1:1031855/150‑1 (MQ=255)
                     aCTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTtg                                                                                                                            <  1:2148433/150‑1 (MQ=255)
                     aCTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTtg                                                                                                                            <  1:1459273/150‑1 (MQ=255)
                      cTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTtgt                                                                                                                           <  2:3245944/150‑1 (MQ=255)
                      cTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTtgt                                                                                                                           >  2:466877/1‑150 (MQ=255)
                        gatgatgaAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTgtgt                                                                                                                         >  1:2973929/1‑150 (MQ=255)
                             tgaAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGAtt                                                                                                                    >  1:1719972/1‑150 (MQ=255)
                               aaGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTg                                                                                                                  >  2:2279720/1‑150 (MQ=255)
                               aaGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTg                                                                                                                  >  2:3863448/1‑150 (MQ=255)
                               aaGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTg                                                                                                                  <  1:3620462/150‑1 (MQ=255)
                                aGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTgg                                                                                                                 <  2:4957674/150‑1 (MQ=255)
                                aGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTgg                                                                                                                 >  2:4598910/1‑150 (MQ=255)
                                   gCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCtt                                                                                                              <  1:1753257/150‑1 (MQ=255)
                                    cAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCttt                                                                                                             >  2:4052411/1‑150 (MQ=255)
                                     aaaaCAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTa                                                                                                            >  1:2802475/1‑150 (MQ=255)
                                        acaacGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATca                                                                                                         >  1:2237964/1‑150 (MQ=255)
                                          aacGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATcaca                                                                                                       >  1:183545/1‑150 (MQ=255)
                                           acGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACCTCTCTTGTGTCGATTTGGCTTTATCACAt                                                                                                      <  2:3174548/150‑1 (MQ=255)
                                            cGTTACCGACAACTATTTCCTCCACTGATCACTCATTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCATAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATa                                                                                                     <  2:3443928/150‑1 (MQ=255)
                                             gTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATag                                                                                                    >  2:1310695/1‑150 (MQ=255)
                                               tACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATagag                                                                                                  <  1:4782873/150‑1 (MQ=255)
                                               tACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATagag                                                                                                  >  2:4854406/1‑150 (MQ=255)
                                                aCCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAACACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGc                                                                                                 <  2:2311001/150‑1 (MQ=255)
                                                  cGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCaa                                                                                               <  1:218274/150‑1 (MQ=255)
                                                       aCTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATg                                                                                          <  2:57934/150‑1 (MQ=255)
                                                        cTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGc                                                                                         >  2:476826/1‑150 (MQ=255)
                                                           tttCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATa                                                                                      <  2:3830078/150‑1 (MQ=255)
                                                             tcctccACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATaaa                                                                                    <  1:3424859/150‑1 (MQ=255)
                                                             tcctccACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATaaa                                                                                    >  2:191329/1‑150 (MQ=255)
                                                              cctccACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTGTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATaaaa                                                                                   >  2:1061946/1‑150 (MQ=255)
                                                                tccACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAAt                                                                                 >  2:519296/1‑150 (MQ=255)
                                                                 ccACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAAtt                                                                                <  1:4081343/150‑1 (MQ=255)
                                                                  cACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCACAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAAttt                                                                               >  2:964978/1‑150 (MQ=255)
                                                                      gATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTa                                                                           >  1:2264779/1‑150 (MQ=255)
                                                                       aTAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTaa                                                                          >  2:4430389/1‑150 (MQ=255)
                                                                       aTAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTaa                                                                          <  2:3665708/150‑1 (MQ=255)
                                                                        tAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTaaa                                                                         >  1:4503607/1‑150 (MQ=255)
                                                                        tAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTaaa                                                                         >  2:2151139/1‑150 (MQ=255)
                                                                           cTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGAGTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATAc                                                                      >  1:3563690/1‑150 (MQ=255)
                                                                             ccTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCg                                                                    <  2:420365/150‑1 (MQ=255)
                                                                              cTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGt                                                                   <  2:659203/150‑1 (MQ=255)
                                                                               tttCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGtt                                                                  <  2:3119135/150‑1 (MQ=255)
                                                                                ttCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGttt                                                                 >  1:1911308/1‑150 (MQ=255)
                                                                                 tCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGtttt                                                                <  1:1426844/150‑1 (MQ=255)
                                                                                    aGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCACCTATGCATAAAAATTTGTTAATTACCGTTTTTTa                                                             >  2:4372687/1‑150 (MQ=255)
                                                                                    aGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTa                                                             >  1:4290504/1‑150 (MQ=255)
                                                                                      cACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAAt                                                           >  2:3536784/1‑150 (MQ=255)
                                                                                       aCGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATc                                                          >  1:3258490/1‑150 (MQ=255)
                                                                                        cGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATcc                                                         <  1:1647843/150‑1 (MQ=255)
                                                                                          cAGTCGCTGGTGCAGTGGCTGCGCGCTTCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGa                                                       <  2:1779959/150‑1 (MQ=255)
                                                                                           aGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAAGCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATGTGTTAAATACCGTTTTTTAATCCgtg                                                      >  1:4942285/1‑148 (MQ=255)
                                                                                            gTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGc                                                     >  2:4124968/1‑150 (MQ=255)
                                                                                              cGCTGGTGCAGTGGCTGCTCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCta                                                   <  1:653074/150‑1 (MQ=255)
                                                                                               gCTGGTGCAGGGGCTGCGAGCTGCAAAATTATCTGCTGTTTTTAAACTTTTTTTAAAGATTATTTCACTTCTATTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCtat                                                  <  1:2637895/150‑1 (MQ=255)
                                                                                                cTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCtcta                                                 >  2:4914249/1‑150 (MQ=255)
                                                                                                cTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCtata                                                 >  2:114384/1‑150 (MQ=255)
                                                                                                cTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCtata                                                 <  2:1537881/150‑1 (MQ=255)
                                                                                                  ggTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGt                                               >  1:386002/1‑150 (MQ=255)
                                                                                                  ggTGCAGTGGCTGCGCGCTCCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGt                                               >  1:2711047/1‑150 (MQ=255)
                                                                                                     gCAGTGGCTGCGCGCTGAAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTTGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGtctc                                            <  1:377707/150‑1 (MQ=255)
                                                                                                       aGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCaa                                          <  2:350905/150‑1 (MQ=255)
                                                                                                         tGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAAc                                        >  2:2762601/1‑150 (MQ=255)
                                                                                                           gCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAAccc                                      <  2:2568582/150‑1 (MQ=255)
                                                                                                           gCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAAccc                                      <  1:4588601/150‑1 (MQ=255)
                                                                                                            cTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCt                                     <  2:3379848/150‑1 (MQ=255)
                                                                                                            cTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCt                                     <  1:1530748/150‑1 (MQ=255)
                                                                                                             tGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTg                                    >  1:504169/1‑150 (MQ=255)
                                                                                                               cgcgcTGCAAAATTATCTGCTGTTTTTAACCTTTT‑TTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCt                                 >  1:525518/1‑150 (MQ=255)
                                                                                                                  gcTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTaa                               <  2:4697625/150‑1 (MQ=255)
                                                                                                                      cAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGtt                           >  1:209036/1‑150 (MQ=255)
                                                                                                                        aaaTTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTAt                         >  1:1108949/1‑150 (MQ=255)
                                                                                                                         aaTTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTAtt                        <  2:2496141/150‑1 (MQ=255)
                                                                                                                         aaTTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTAtt                        <  2:1666184/150‑1 (MQ=255)
                                                                                                                          aTTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATACGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTc                       >  1:3359541/1‑150 (MQ=255)
                                                                                                                           ttATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTca                      <  1:829166/150‑2 (MQ=255)
                                                                                                                           ttATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCt                      <  1:2486151/150‑1 (MQ=255)
                                                                                                                           ttATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCt                      >  2:2379500/1‑150 (MQ=255)
                                                                                                                            tATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCtt                     >  2:2868923/1‑150 (MQ=255)
                                                                                                                            tATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCtt                     <  2:771148/150‑1 (MQ=255)
                                                                                                                               ctgctgTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCg                  <  2:4661082/150‑1 (MQ=255)
                                                                                                                                 gctgTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGAt                >  2:400003/1‑150 (MQ=255)
                                                                                                                                    gTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCt             <  2:2477777/150‑1 (MQ=255)
                                                                                                                                       tttAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCtttt          <  2:2973929/150‑1 (MQ=255)
                                                                                                                                       tttAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCtttt          >  2:896467/1‑150 (MQ=255)
                                                                                                                                       tttAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCtatt          >  2:3416701/1‑150 (MQ=255)
                                                                                                                                         tAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTtat        >  2:2714173/1‑150 (MQ=255)
                                                                                                                                               ttttttAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCg  <  2:3077267/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCG  >  CP009273/888818‑889110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATGCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATA  >  CP009273/888815‑889107
                                                                                                                                                     |                                                                                                                                               
AATGCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTT                                                                                                                                                 <  SRR19973063.4501872/150‑1 (MQ=60)
AATGCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTT                                                                                                                                                 <  SRR19973063.2242805/150‑1 (MQ=60)
AATGCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTT                                                                                                                                                 <  SRR19973063.4501869/150‑1 (MQ=60)
AATGCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTT                                                                                                                                                 <  SRR19973063.1171601/150‑1 (MQ=60)
AATGCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTT                                                                                                                                                 <  SRR19973063.3066254/150‑1 (MQ=60)
 ATGCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTT                                                                                                                                                <  SRR19973063.1213415/150‑1 (MQ=60)
 ATGCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTT                                                                                                                                                <  SRR19973063.3904728/150‑1 (MQ=60)
    CCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAA                                                                                                                                             <  SRR19973063.4154058/150‑1 (MQ=60)
     CCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAA                                                                                                                                            >  SRR19973063.2485701/1‑150 (MQ=60)
      CATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAG                                                                                                                                           <  SRR19973063.1321502/150‑1 (MQ=60)
       ATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGA                                                                                                                                          <  SRR19973063.1067392/150‑1 (MQ=60)
       ATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGA                                                                                                                                          >  SRR19973063.1084659/1‑150 (MQ=60)
         CAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATT                                                                                                                                        <  SRR19973063.1489660/150‑1 (MQ=60)
         CAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATT                                                                                                                                        >  SRR19973063.3087726/1‑150 (MQ=60)
         CAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATT                                                                                                                                        >  SRR19973063.3087155/1‑150 (MQ=60)
            CTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATT                                                                                                                                     >  SRR19973063.4905664/1‑150 (MQ=60)
             TGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTT                                                                                                                                    >  SRR19973063.250876/1‑150 (MQ=60)
             TGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTT                                                                                                                                    <  SRR19973063.1106139/150‑1 (MQ=60)
             TGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTT                                                                                                                                    >  SRR19973063.250920/1‑150 (MQ=60)
               CTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCA                                                                                                                                  <  SRR19973063.2327518/150‑1 (MQ=60)
               CTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCA                                                                                                                                  <  SRR19973063.4895700/150‑1 (MQ=60)
                TGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCAC                                                                                                                                 <  SRR19973063.4589489/150‑1 (MQ=60)
                  TTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTCTCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTT                                                                                                                               <  SRR19973063.3953016/150‑1 (MQ=60)
                  TTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTT                                                                                                                               <  SRR19973063.46610/150‑1 (MQ=60)
                    ACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCT                                                                                                                             <  SRR19973063.3494123/150‑1 (MQ=60)
                        ACTGATGATGAAGTGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTG                                                                                                                         <  SRR19973063.2594573/150‑1 (MQ=60)
                        ACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTG                                                                                                                         <  SRR19973063.1034971/150‑1 (MQ=60)
                        ACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTG                                                                                                                         <  SRR19973063.2155162/150‑1 (MQ=60)
                        ACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTG                                                                                                                         >  SRR19973063.223091/1‑150 (MQ=60)
                        ACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTG                                                                                                                         <  SRR19973063.1463726/150‑1 (MQ=60)
                        ACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTG                                                                                                                         <  SRR19973063.1463700/150‑1 (MQ=60)
                           GATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGT                                                                                                                      >  SRR19973063.2983433/1‑150 (MQ=60)
                                TGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATT                                                                                                                 >  SRR19973063.1725220/1‑150 (MQ=60)
                                 GAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGAGTT                                                                                                                >  SRR19973063.3505521/1‑150 (MQ=60)
                                  AAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTG                                                                                                               <  SRR19973063.3632079/150‑1 (MQ=60)
                                   AGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGG                                                                                                              <  SRR19973063.392482/150‑1 (MQ=60)
                                    GGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGC                                                                                                             <  SRR19973063.3588738/150‑1 (MQ=60)
                                      GCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTT                                                                                                           <  SRR19973063.1758639/150‑1 (MQ=60)
                                        AAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTA                                                                                                         >  SRR19973063.179008/1‑150 (MQ=60)
                                        AAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTA                                                                                                         >  SRR19973063.2811483/1‑150 (MQ=60)
                                        AAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTA                                                                                                         >  SRR19973063.2788017/1‑150 (MQ=60)
                                           ACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCA                                                                                                      >  SRR19973063.2245068/1‑150 (MQ=60)
                                             AACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACA                                                                                                    >  SRR19973063.184113/1‑150 (MQ=60)
                                                  TACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAG                                                                                               <  SRR19973063.3438751/150‑1 (MQ=60)
                                                  TACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAG                                                                                               >  SRR19973063.4733937/1‑150 (MQ=60)
                                                  TACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAG                                                                                               <  SRR19973063.4798148/150‑1 (MQ=60)
                                                     CGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAA                                                                                            <  SRR19973063.218931/150‑1 (MQ=60)
                                                     CGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAA                                                                                            <  SRR19973063.218894/150‑1 (MQ=60)
                                                       AAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAAT                                                                                          >  SRR19973063.205177/1‑150 (MQ=60)
                                                       AAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAAT                                                                                          >  SRR19973063.2213344/1‑150 (MQ=60)
                                                                TCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAA                                                                                 <  SRR19973063.3435753/150‑1 (MQ=60)
                                                                    CCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATT                                                                             >  SRR19973063.573101/1‑150 (MQ=60)
                                                                    CCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATT                                                                             <  SRR19973063.4094412/150‑1 (MQ=60)
                                                                     CACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGAGTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTT                                                                            >  SRR19973063.3048526/1‑150 (MQ=60)
                                                                      ACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTG                                                                           >  SRR19973063.3876025/1‑150 (MQ=60)
                                                                        TGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTT                                                                         >  SRR19973063.1743752/1‑150 (MQ=60)
                                                                         GATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTA                                                                        >  SRR19973063.2271995/1‑150 (MQ=60)
                                                                         GATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTA                                                                        <  SRR19973063.1632771/150‑1 (MQ=60)
                                                                           TAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAA                                                                      >  SRR19973063.4518063/1‑150 (MQ=60)
                                                                           TAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAA                                                                      >  SRR19973063.4518068/1‑150 (MQ=60)
                                                                             TCTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATA                                                                    >  SRR19973063.4074986/1‑150 (MQ=60)
                                                                              CTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATAC                                                                   >  SRR19973063.747329/1‑150 (MQ=60)
                                                                              CTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGAGTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATAC                                                                   >  SRR19973063.3575116/1‑150 (MQ=60)
                                                                                   TTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATATAGCAAATATGCATAAAAATTTGTTAAATAACGTTT                                                              >  SRR19973063.1917289/1‑150 (MQ=60)
                                                                                   TTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTT                                                              >  SRR19973063.1917256/1‑150 (MQ=60)
                                                                                    TCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTT                                                             <  SRR19973063.1431192/150‑1 (MQ=60)
                                                                                     CGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTT                                                            >  SRR19973063.3417708/1‑150 (MQ=60)
                                                                                      GAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTT                                                           <  SRR19973063.2799031/150‑1 (MQ=60)
                                                                                       AGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTA                                                          <  SRR19973063.3055391/150‑1 (MQ=60)
                                                                                       AGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTA                                                          >  SRR19973063.4304136/1‑150 (MQ=60)
                                                                                         CACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAAT                                                        >  SRR19973063.4424319/1‑150 (MQ=60)
                                                                                         CACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAAT                                                        <  SRR19973063.1716488/150‑1 (MQ=60)
                                                                                          ACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACCTAGAGCAAATATGCATAAAAAGTTGTTATATACCGTTTTTTAATC                                                       >  SRR19973063.1966772/1‑150 (MQ=60)
                                                                                          ACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATC                                                       >  SRR19973063.3268948/1‑150 (MQ=60)
                                                                                           CGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCC                                                      <  SRR19973063.1652876/150‑1 (MQ=60)
                                                                                           CGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCC                                                      <  SRR19973063.5126898/150‑1 (MQ=60)
                                                                                             CAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTATTGATTCGATTTGGATTTATCACATATAGCCAATATTAATAAAAATTATTTAAATACAGTTTTTTAATACGA                                                    >  SRR19973063.1762658/1‑150 (MQ=60)
                                                                                              AGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAAGCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATGTGTTAAATACCGTTTTTTAATCCGTG                                                   >  SRR19973063.4958269/1‑150 (MQ=60)
                                                                                                 CGCTGGTGCAGTGGCTGCTCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTA                                                <  SRR19973063.654989/150‑1 (MQ=60)
                                                                                                 CGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTA                                                <  SRR19973063.654760/150‑1 (MQ=60)
                                                                                                  GCTGGTGCAGGGGCTGCGAGCTGCAAAATTATCTGCTGTTTTTAAACTTTTTTTAAAGATTATTTCACTTCTATTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTAT                                               <  SRR19973063.2646316/150‑1 (MQ=60)
                                                                                                     GGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGT                                            >  SRR19973063.387101/1‑150 (MQ=60)
                                                                                                     GGTGCAGTGGCTGCGCGCTCCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGT                                            >  SRR19973063.2719805/1‑150 (MQ=60)
                                                                                                        GCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTC                                         <  SRR19973063.378775/150‑1 (MQ=60)
                                                                                                        GCAGTGGCTGCGCGCTGAAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTTGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTC                                         <  SRR19973063.378785/150‑1 (MQ=60)
                                                                                                          AGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAA                                       >  SRR19973063.4717587/1‑150 (MQ=60)
                                                                                                          AGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAA                                       >  SRR19973063.356828/1‑150 (MQ=60)
                                                                                                            TGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAAC                                     >  SRR19973063.4615759/1‑150 (MQ=60)
                                                                                                              GCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCC                                   <  SRR19973063.4603283/150‑1 (MQ=60)
                                                                                                               CTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCT                                  <  SRR19973063.1535444/150‑1 (MQ=60)
                                                                                                               CTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCT                                  <  SRR19973063.1535410/150‑1 (MQ=60)
                                                                                                                TGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTG                                 >  SRR19973063.505628/1‑150 (MQ=60)
                                                                                                                  CGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTT‑TTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCT                              >  SRR19973063.527057/1‑150 (MQ=60)
                                                                                                                      CTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAA                           >  SRR19973063.4235432/1‑150 (MQ=60)
                                                                                                                         CAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTT                        >  SRR19973063.209664/1‑150 (MQ=60)
                                                                                                                           AAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTAT                      >  SRR19973063.496153/1‑150 (MQ=60)
                                                                                                                           AAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTAT                      >  SRR19973063.1112320/1‑150 (MQ=60)
                                                                                                                             ATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAATCCCTGGCTAAAGTTATTC                    >  SRR19973063.3370076/1‑150 (MQ=60)
                                                                                                                             ATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTCATTC                    >  SRR19973063.3370131/1‑150 (MQ=60)
                                                                                                                             ATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATACGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTC                    >  SRR19973063.3370237/1‑150 (MQ=60)
                                                                                                                              TTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCT                   <  SRR19973063.2494096/150‑1 (MQ=60)
                                                                                                                              TTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCT                   <  SRR19973063.2494129/150‑1 (MQ=60)
                                                                                                                              TTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCT                   <  SRR19973063.1416742/150‑1 (MQ=60)
                                                                                                                              TTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCT                   >  SRR19973063.2930621/1‑150 (MQ=60)
                                                                                                                              TTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCA                   <  SRR19973063.831561/150‑1 (MQ=60)
                                                                                                                               TATCTGCTGTCTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGAATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTT                  >  SRR19973063.166693/1‑150 (MQ=60)
                                                                                                                                     CTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATG            >  SRR19973063.3765084/1‑150 (MQ=60)
                                                                                                                                     CTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCTTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATG            >  SRR19973063.2100515/1‑150 (MQ=60)
                                                                                                                                      TGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGC           <  SRR19973063.3813999/150‑1 (MQ=60)
                                                                                                                                         TTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTT        <  SRR19973063.2248664/150‑1 (MQ=60)
                                                                                                                                         TTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGAAAATATGCATAAAAATTTGTTAAATAACGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTT        >  SRR19973063.5039772/1‑150 (MQ=60)
                                                                                                                                          TTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGNGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCATAGCTATAGTCTCAAAACCTGTCTAAAATTATTCTTGCGATGCTTTT       >  SRR19973063.2683590/1‑150 (MQ=60)
                                                                                                                                               CCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATA  <  SRR19973063.4870048/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                               
AATGCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATA  >  CP009273/888815‑889107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑