Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 888,964 C→T 100% intergenic (+75/‑276) ybjN → / → potF negative regulator of motility; multicopy suppressor of coaA(Ts)/putrescine transporter subunit: periplasmic‑binding component of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273888,9640CT97.3% 706.9 / 7.5 218intergenic (+75/‑276)ybjN/potFnegative regulator of motility; multicopy suppressor of coaA(Ts)/putrescine transporter subunit: periplasmic‑binding component of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base C (4/2);  new base T (94/118);  total (98/120)
Fisher's exact test for biased strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 219 total aligned reads displayed.

CCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATA  >  CP009273/888820‑889107
                                                                                                                                                |                                                                                                                                               
ccATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTa                                                                                                                                              >  1:2050407/1‑148 (MQ=255)
 cATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTaa                                                                                                                                             <  1:3326248/148‑1 (MQ=255)
  aTCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTaaa                                                                                                                                            >  2:2353906/1‑148 (MQ=255)
      aCTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGatt                                                                                                                                        <  1:1139357/148‑1 (MQ=255)
      aCTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGatt                                                                                                                                        >  1:1458666/1‑148 (MQ=255)
       ctgctgTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGatta                                                                                                                                       <  2:3381195/148‑1 (MQ=255)
       ctgctgTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGatta                                                                                                                                       <  2:1613680/148‑1 (MQ=255)
        tgctgTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGattat                                                                                                                                      >  2:678108/1‑148 (MQ=255)
            gTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCa                                                                                                                                  >  1:1366548/1‑148 (MQ=255)
              tACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACt                                                                                                                                >  1:1801015/1‑148 (MQ=255)
               aCCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACtt                                                                                                                               >  2:1992714/1‑148 (MQ=255)
               aCCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACtt                                                                                                                               >  2:2046542/1‑148 (MQ=255)
               aCCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACtt                                                                                                                               >  2:1051185/1‑148 (MQ=255)
                     tgatgatgaAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTtg                                                                                                                         <  2:933131/148‑1 (MQ=255)
                     tgatgatgaAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTtg                                                                                                                         <  2:693899/148‑1 (MQ=255)
                        tgatgaAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTgtgt                                                                                                                      <  1:760197/148‑1 (MQ=255)
                          atgaAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCg                                                                                                                    <  1:393107/148‑1 (MQ=255)
                           tgaAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGa                                                                                                                   <  2:1163860/148‑1 (MQ=255)
                           tgaAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGa                                                                                                                   <  2:3391056/148‑1 (MQ=255)
                               gggCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTg                                                                                                               >  2:571320/1‑148 (MQ=255)
                                ggCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTgg                                                                                                              >  1:1355368/1‑148 (MQ=255)
                                ggCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTgg                                                                                                              <  1:2255074/148‑1 (MQ=255)
                                  cAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCt                                                                                                            >  2:3235999/1‑148 (MQ=255)
                                     aacaacGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCAc                                                                                                                                 <  1:2754651/124‑1 (MQ=255)
                                     aacaacGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTa                                                                                                         <  1:3150210/148‑1 (MQ=255)
                                     aacaacGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTa                                                                                                         <  2:2758693/148‑1 (MQ=255)
                                        aacGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATca                                                                                                      >  1:1008712/1‑148 (MQ=255)
                                        aacGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATca                                                                                                      >  1:2520415/1‑148 (MQ=255)
                                        aacGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTGTATca                                                                                                      >  2:2796422/1‑148 (MQ=255)
                                          cGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATcaca                                                                                                    <  1:860552/148‑1 (MQ=255)
                                          cGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATcaca                                                                                                    >  2:222183/1‑148 (MQ=255)
                                              aCCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATaga                                                                                                >  2:1955701/1‑148 (MQ=255)
                                              aCCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATaga                                                                                                >  1:1434443/1‑148 (MQ=255)
                                               ccGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTATAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATagag                                                                                               >  1:1277452/1‑148 (MQ=255)
                                               ccGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATagag                                                                                               >  1:1335869/1‑148 (MQ=255)
                                                cGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGc                                                                                              >  2:2498651/1‑148 (MQ=255)
                                                   aaaCTATTTCCTCCACTGATCACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCaaa                                                                                           <  2:802365/148‑1 (MQ=255)
                                                   aaaCTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTGTCTTGTGTCGATTTGGCTTTATCACATAGAGCaaa                                                                                           >  1:2125549/1‑148 (MQ=255)
                                                   aaaCTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCaaa                                                                                           >  2:2667031/1‑148 (MQ=255)
                                                    aaCTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAat                                                                                          >  1:682200/1‑148 (MQ=255)
                                                    aaCTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAat                                                                                          <  2:652800/148‑1 (MQ=255)
                                                     aCTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAata                                                                                         <  2:1677723/148‑1 (MQ=255)
                                                     aCTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAata                                                                                         >  1:104262/1‑148 (MQ=255)
                                                          ttCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCAt                                                                                    <  1:1917013/148‑1 (MQ=255)
                                                          ttCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCAt                                                                                    >  2:3343245/1‑148 (MQ=255)
                                                              tccACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATaaaa                                                                                >  1:2651939/1‑148 (MQ=255)
                                                               ccACTGATAACTCCTTTCGAGCACGCAGTCGCGGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTTACCTTTTTTTAAAGATTATTTGACTTCTCTTGTGTCGATTTTGGTTTATCACATAGAGCAAAAATGGATaaaaa                                                                               >  1:1521070/1‑148 (MQ=255)
                                                                 aCTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAAtt                                                                             <  2:475312/148‑1 (MQ=255)
                                                                  cTGATAACTCCTTTCGAGCTCGCTGTCTCTGGTTCAGTGGCTGCGCGCTGCAAAATTATCTGCTGATTTGAACCTTTTTTTAAAGATTTTTTCACTTCTATTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAAttt                                                                            <  1:3235087/148‑1 (MQ=255)
                                                                  cTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAAttt                                                                            <  1:2903983/148‑1 (MQ=255)
                                                                     aTAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGtt                                                                         >  1:1667037/1‑148 (MQ=255)
                                                                     aTAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAAGTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGtt                                                                         >  1:1667043/1‑148 (MQ=255)
                                                                       aaCTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTGTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAAAATGCATAAAAATTTGTTaa                                                                       >  1:1841860/1‑148 (MQ=255)
                                                                       aaCTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTaa                                                                       >  2:1853393/1‑148 (MQ=255)
                                                                       aaCTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTaa                                                                       <  1:1048502/148‑1 (MQ=255)
                                                                        aCTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTaaa                                                                      >  1:3101794/1‑148 (MQ=255)
                                                                         cTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAAt                                                                     <  2:205820/148‑1 (MQ=255)
                                                                          tCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATa                                                                    <  2:360521/148‑1 (MQ=255)
                                                                           ccTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATAc                                                                   >  2:2316262/1‑148 (MQ=255)
                                                                             tttCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGGTGCAAAATTATCTGCTGTTTTTATCCTTTTTTTAAAGATTATTTCACTTCTATTGTGTCGATTTGGCTTTATCACATTGTGCAAATATGCATATAAATTTGTTAAATACCg                                                                 <  2:1739546/148‑1 (MQ=255)
                                                                             tttCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCg                                                                 <  1:716289/148‑1 (MQ=255)
                                                                               tCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGtt                                                               <  2:200375/148‑1 (MQ=255)
                                                                               tCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGtt                                                               <  2:1304192/148‑1 (MQ=255)
                                                                                      cGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAAt                                                        <  2:79564/148‑1 (MQ=255)
                                                                                         aGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCg                                                     <  1:1832390/148‑1 (MQ=255)
                                                                                          gTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGa                                                    <  1:2219858/148‑1 (MQ=255)
                                                                                           tCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCTTTTTTTAATCCGAg                                                   >  1:3052152/1‑148 (MQ=255)
                                                                                              cTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCta                                                <  2:1169156/148‑1 (MQ=255)
                                                                                              cTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCta                                                <  2:2251542/148‑1 (MQ=255)
                                                                                                ggTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCtata                                              <  2:1524711/148‑1 (MQ=255)
                                                                                                 gTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAg                                             <  1:2770720/148‑1 (MQ=255)
                                                                                                  tGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGt                                            <  2:58774/148‑1 (MQ=255)
                                                                                                        ggCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCaaa                                      <  1:511576/148‑1 (MQ=255)
                                                                                                        ggCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCaaa                                      >  2:3311894/1‑148 (MQ=255)
                                                                                                         gCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAAc                                     <  1:171542/148‑1 (MQ=255)
                                                                                                         gCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAAc                                     >  1:1655385/1‑148 (MQ=255)
                                                                                                          cTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAAcc                                    <  2:2205832/148‑1 (MQ=255)
                                                                                                          cTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAAcc                                    <  1:1992718/148‑1 (MQ=255)
                                                                                                          cTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAAcc                                    <  2:2589857/148‑1 (MQ=255)
                                                                                                             cgcgcTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATACGAGCTATAGTCTCAAACCATg                                 >  2:3071839/1‑148 (MQ=255)
                                                                                                               cgcTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGc                               <  1:1010110/148‑1 (MQ=255)
                                                                                                                  tGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTaa                            <  1:2536570/148‑1 (MQ=255)
                                                                                                                  tGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTaa                            >  1:2398412/1‑148 (MQ=255)
                                                                                                                  tGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTaa                            >  1:3552321/1‑148 (MQ=255)
                                                                                                                       aaTTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTa                       <  2:1594092/148‑1 (MQ=255)
                                                                                                                         ttATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTAtt                     <  2:745303/148‑1 (MQ=255)
                                                                                                                          tATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTc                    >  1:3458187/1‑148 (MQ=255)
                                                                                                                             ctgctgTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTg                 <  1:867402/148‑1 (MQ=255)
                                                                                                                               gctgTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCg               >  1:849667/1‑148 (MQ=255)
                                                                                                                               gctgTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATACTTGCg               >  2:3194721/1‑148 (MQ=255)
                                                                                                                                ctgTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTTTTAAATACCATTTTTTAATCCGAGCTATATTCTCAAACCCTTGCTAAAGTTATTCTTGCGa              >  1:3285730/1‑148 (MQ=255)
                                                                                                                                ctgTTTTTAACCATTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGa              <  2:1157909/148‑1 (MQ=255)
                                                                                                                                     tttAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCtt         <  2:34624/148‑1 (MQ=255)
                                                                                                                                     tttAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCtt         <  2:1716192/148‑1 (MQ=255)
                                                                                                                                      ttAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCttt        <  1:3054531/148‑1 (MQ=255)
                                                                                                                                        aaCCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTta      <  2:1109361/148‑1 (MQ=255)
                                                                                                                                        aaCCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTta      <  2:2887342/148‑1 (MQ=255)
                                                                                                                                          ccTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTtata    >  2:545311/1‑148 (MQ=255)
                                                                                                                                          ccTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTtata    <  1:2921686/148‑1 (MQ=255)
                                                                                                                                            ttttttTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTtatata  >  1:2150508/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATA  >  CP009273/888820‑889107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATGCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGC  >  CP009273/888815‑889113
                                                                                                                                                     |                                                                                                                                                     
AATGCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTT                                                                                                                                                       >  SRR19973066.2607403/1‑150 (MQ=60)
  TGCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTT                                                                                                                                                     <  SRR19973066.3359468/150‑1 (MQ=60)
   GCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTA                                                                                                                                                    >  SRR19973066.2064657/1‑150 (MQ=60)
      CATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAG                                                                                                                                                 <  SRR19973066.3348348/150‑1 (MQ=60)
      CATCAACTGCTGTTACAGACTGATGATGTAGGGCAAAACAACGTTACCGATAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTTCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAG                                                                                                                                                 <  SRR19973066.3348222/150‑1 (MQ=60)
       ATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGA                                                                                                                                                <  SRR19973066.1667485/150‑1 (MQ=60)
       ATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGA                                                                                                                                                <  SRR19973066.3527537/150‑1 (MQ=60)
       ATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGA                                                                                                                                                >  SRR19973066.805670/1‑150 (MQ=60)
         CAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATT                                                                                                                                              >  SRR19973066.1468996/1‑150 (MQ=60)
         CAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATT                                                                                                                                              >  SRR19973066.1468912/1‑150 (MQ=60)
           ACTGCTGTTACCGTCTGATGATGTAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTAT                                                                                                                                            <  SRR19973066.1147505/150‑1 (MQ=60)
               CTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCA                                                                                                                                        >  SRR19973066.1376432/1‑150 (MQ=60)
                 GTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACT                                                                                                                                      >  SRR19973066.1813815/1‑150 (MQ=60)
                 GTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACT                                                                                                                                      <  SRR19973066.2727730/150‑1 (MQ=60)
                 GNTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACT                                                                                                                                      >  SRR19973066.1814244/1‑150 (MQ=60)
                  cagacgtgtgctcttccgatctAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTT                                                                                                                                     <  SRR19973066.2773200/128‑1 (MQ=60)
                             TGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCG                                                                                                                          <  SRR19973066.765829/150‑1 (MQ=60)
                               ATGAAGGTCAAAACAAAGTTACCGAAAACTATTTACTCCACTGATAACTCCTTTCGAGCACGCCGTCGCTTGTGCAGTGGCTGCGCGCTGAAAATTTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGAT                                                                                                                        <  SRR19973066.396082/150‑1 (MQ=60)
                               ATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGAT                                                                                                                        <  SRR19973066.396206/150‑1 (MQ=60)
                               ATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGAT                                                                                                                        >  SRR19973066.1527563/1‑150 (MQ=60)
                                   AGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGG                                                                                                                    >  SRR19973066.1364291/1‑150 (MQ=60)
                                   AGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGG                                                                                                                    >  SRR19973066.1365184/1‑150 (MQ=60)
                                   AGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGG                                                                                                                    >  SRR19973066.209076/1‑150 (MQ=60)
                                     GGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCT                                                                                                                  <  SRR19973066.2270648/150‑1 (MQ=60)
                                     GGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCT                                                                                                                  <  SRR19973066.2270618/150‑1 (MQ=60)
                                      GCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTT                                                                                                                 <  SRR19973066.2810220/150‑1 (MQ=60)
                                       CAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTT                                                                                                                >  SRR19973066.293125/1‑150 (MQ=60)
                                          AACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATC                                                                                                             <  SRR19973066.3171210/150‑1 (MQ=60)
                                          AACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATC                                                                                                             <  SRR19973066.3171214/150‑1 (MQ=60)
                                           ACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCA                                                                                                            >  SRR19973066.2537557/1‑150 (MQ=60)
                                           ACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCA                                                                                                            >  SRR19973066.1016140/1‑150 (MQ=60)
                                           ACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCA                                                                                                            <  SRR19973066.758812/150‑1 (MQ=60)
                                           ACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCA                                                                                                            >  SRR19973066.2522473/1‑150 (MQ=60)
                                            CAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCAC                                                                                                           >  SRR19973066.3410445/1‑150 (MQ=60)
                                            CAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCAC                                                                                                           <  SRR19973066.3144903/150‑1 (MQ=60)
                                               CGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATA                                                                                                        <  SRR19973066.866745/150‑1 (MQ=60)
                                                 TTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGA                                                                                                      >  SRR19973066.1444672/1‑150 (MQ=60)
                                                  TACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTATAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAG                                                                                                     >  SRR19973066.1286675/1‑150 (MQ=60)
                                                  TACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAG                                                                                                     >  SRR19973066.1345563/1‑150 (MQ=60)
                                                    CCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCA                                                                                                   >  SRR19973066.3221715/1‑150 (MQ=60)
                                                    CCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCA                                                                                                   >  SRR19973066.2193537/1‑150 (MQ=60)
                                                    CCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCA                                                                                                   <  SRR19973066.1284830/150‑1 (MQ=60)
                                                    CCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCA                                                                                                   <  SRR19973066.1335912/150‑1 (MQ=60)
                                                    CCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCA                                                                                                   >  SRR19973066.386417/1‑150 (MQ=60)
                                                      GAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTGTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAA                                                                                                 >  SRR19973066.2140266/1‑150 (MQ=60)
                                                       AAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAAT                                                                                                >  SRR19973066.687212/1‑150 (MQ=60)
                                                        AAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATA                                                                                               >  SRR19973066.105401/1‑150 (MQ=60)
                                                        AAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATA                                                                                               >  SRR19973066.105356/1‑150 (MQ=60)
                                                           CTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGC                                                                                            <  SRR19973066.3072579/150‑1 (MQ=60)
                                                            TATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCA                                                                                           <  SRR19973066.2638637/150‑1 (MQ=60)
                                                              TTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATA                                                                                         <  SRR19973066.2252144/150‑1 (MQ=60)
                                                               TTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAA                                                                                        <  SRR19973066.1930656/150‑1 (MQ=60)
                                                                TCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATTCATAAA                                                                                       >  SRR19973066.1327260/1‑150 (MQ=60)
                                                                 CCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAA                                                                                      >  SRR19973066.2669739/1‑150 (MQ=60)
                                                                  CTCCACTGATAACTCCTTTCGAGCACGCAGTCGCGGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTTACCTTTTTTTAAAGATTATTTGACTTCTCTTGTGTCGATTTTGGTTTATCACATAGAGCAAAAATGGATAAAAA                                                                                     >  SRR19973066.1531798/1‑150 (MQ=60)
                                                                     CACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTT                                                                                  <  SRR19973066.1899760/150‑1 (MQ=60)
                                                                       CTGATAACTCCTTTCGAGCTCGCTGTCTCTGGTTCAGTGGCTGCGCGCTGCAAAATTATCTGCTGATTTGAACCTTTTTTTAAAGATTTTTTCACTTCTATTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGT                                                                                <  SRR19973066.3256450/150‑1 (MQ=60)
                                                                       CTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGT                                                                                <  SRR19973066.2923382/150‑1 (MQ=60)
                                                                       CTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGT                                                                                <  SRR19973066.2922852/150‑1 (MQ=60)
                                                                        TGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTT                                                                               >  SRR19973066.1679185/1‑150 (MQ=60)
                                                                        TGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACAGAGAGCAAATATGCATAAAAATTTGTT                                                                               >  SRR19973066.1679186/1‑150 (MQ=60)
                                                                        TGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAAGTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTT                                                                               >  SRR19973066.1679191/1‑150 (MQ=60)
                                                                         GATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTA                                                                              >  SRR19973066.255015/1‑150 (MQ=60)
                                                                         GATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTA                                                                              <  SRR19973066.2151503/150‑1 (MQ=60)
                                                                          ATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTGTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAAAATGCATAAAAATTTGTTAA                                                                             >  SRR19973066.1855024/1‑150 (MQ=60)
                                                                          ATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAA                                                                             >  SRR19973066.1854641/1‑150 (MQ=60)
                                                                           TAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAA                                                                            >  SRR19973066.3122508/1‑150 (MQ=60)
                                                                            AACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAAT                                                                           <  SRR19973066.1056209/150‑1 (MQ=60)
                                                                              CTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATAC                                                                         >  SRR19973066.711290/1‑150 (MQ=60)
                                                                                CCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCG                                                                       >  SRR19973066.2102379/1‑150 (MQ=60)
                                                                                 CTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCCTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGT                                                                      <  SRR19973066.1020545/150‑1 (MQ=60)
                                                                                  TTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTT                                                                     <  SRR19973066.2521140/150‑1 (MQ=60)
                                                                                  TTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTT                                                                     <  SRR19973066.721592/150‑1 (MQ=60)
                                                                                              AGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAG                                                         <  SRR19973066.1845477/150‑1 (MQ=60)
                                                                                              AGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCTTTTTTTAATCCGAG                                                         >  SRR19973066.3072564/1‑150 (MQ=60)
                                                                                               GTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGC                                                        <  SRR19973066.2235215/150‑1 (MQ=60)
                                                                                                TCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCT                                                       >  SRR19973066.90965/1‑150 (MQ=60)
                                                                                                    TGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAG                                                   >  SRR19973066.1495709/1‑150 (MQ=60)
                                                                                                      GTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATTAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTC                                                 <  SRR19973066.2776989/150‑1 (MQ=60)
                                                                                                      GTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTC                                                 <  SRR19973066.2789377/150‑1 (MQ=60)
                                                                                                            TGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAAC                                           >  SRR19973066.1667445/1‑150 (MQ=60)
                                                                                                             GGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACC                                          <  SRR19973066.515360/150‑1 (MQ=60)
                                                                                                              GCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCC                                         <  SRR19973066.173073/150‑1 (MQ=60)
                                                                                                               CTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCT                                        >  SRR19973066.551902/1‑150 (MQ=60)
                                                                                                               CTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCT                                        <  SRR19973066.2006736/150‑1 (MQ=60)
                                                                                                               CTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCT                                        <  SRR19973066.2006740/150‑1 (MQ=60)
                                                                                                                TGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTG                                       <  SRR19973066.1794955/150‑1 (MQ=60)
                                                                                                                TGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTG                                       <  SRR19973066.1794249/150‑1 (MQ=60)
                                                                                                                   GCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCT                                    <  SRR19973066.3257364/150‑1 (MQ=60)
                                                                                                                    CGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTA                                   <  SRR19973066.1017536/150‑1 (MQ=60)
                                                                                                                    CGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTA                                   <  SRR19973066.1017555/150‑1 (MQ=60)
                                                                                                                     GCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAA                                  >  SRR19973066.3575891/1‑150 (MQ=60)
                                                                                                                     GCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAA                                  >  SRR19973066.2414591/1‑150 (MQ=60)
                                                                                                                       TGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAG                                <  SRR19973066.2553810/150‑1 (MQ=60)
                                                                                                                           AAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTAT                            >  SRR19973066.471974/1‑150 (MQ=60)
                                                                                                                            AATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATT                           >  SRR19973066.163141/1‑150 (MQ=60)
                                                                                                                             ATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTC                          >  SRR19973066.3481310/1‑150 (MQ=60)
                                                                                                                             ATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTC                          >  SRR19973066.1373413/1‑150 (MQ=60)
                                                                                                                                 TCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGC                      <  SRR19973066.858662/150‑1 (MQ=60)
                                                                                                                                  CTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAGATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCG                     <  SRR19973066.873092/150‑1 (MQ=60)
                                                                                                                                  CTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCG                     <  SRR19973066.873624/150‑1 (MQ=60)
                                                                                                                                  CTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCG                     >  SRR19973066.855818/1‑150 (MQ=60)
                                                                                                                                  CTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCG                     >  SRR19973066.855777/1‑150 (MQ=60)
                                                                                                                                   TGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTTTTAAATACCATTTTTTAATCCGAGCTATATTCTCAAACCCTTGCTAAAGTTATTCTTGCGA                    >  SRR19973066.3307371/1‑150 (MQ=60)
                                                                                                                                    GCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGAT                   <  SRR19973066.2052931/150‑1 (MQ=60)
                                                                                                                                    GCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGAT                   >  SRR19973066.3055882/1‑150 (MQ=60)
                                                                                                                                     CTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATG                  <  SRR19973066.3103090/150‑1 (MQ=60)
                                                                                                                                          TTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTT             <  SRR19973066.1082322/150‑1 (MQ=60)
                                                                                                                                           TTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTA            <  SRR19973066.3074960/150‑1 (MQ=60)
                                                                                                                                              ACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATAT         >  SRR19973066.1407922/1‑150 (MQ=60)
                                                                                                                                               CCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATA        >  SRR19973066.2165437/1‑150 (MQ=60)
                                                                                                                                               CCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATA        <  SRR19973066.2941163/150‑1 (MQ=60)
                                                                                                                                                CTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAG       <  SRR19973066.1709399/150‑1 (MQ=60)
                                                                                                                                                 TTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTATCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGC      >  SRR19973066.1067228/1‑150 (MQ=60)
                                                                                                                                                    TTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAG   >  SRR19973066.496232/1‑150 (MQ=60)
                                                                                                                                                     TTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGC  >  SRR19973066.519006/1‑150 (MQ=60)
                                                                                                                                                     TTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGC  >  SRR19973066.1295435/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AATGCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAGCGAGC  >  CP009273/888815‑889113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑