Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,411,250 G→T 100% E953D (GAG→GAT)  acrF → multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,411,2500GT99.0% 654.8 / ‑2.0 209E953D (GAG→GAT) acrFmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (112/95);  total (114/95)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 209 total aligned reads displayed.

CGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCT  >  CP009273/3411105‑3411394
                                                                                                                                                 |                                                                                                                                                
   tAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAaga                                                                                                                                             <  2:2823647/148‑1 (MQ=255)
      ggATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAggg                                                                                                                                          >  2:582994/1‑148 (MQ=255)
      ggATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAAACAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAggg                                                                                                                                          >  1:1236114/1‑148 (MQ=255)
       gATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGt                                                                                                                                         <  1:578757/148‑1 (MQ=255)
       gATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGt                                                                                                                                         <  1:2364959/148‑1 (MQ=255)
        aTTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTa                                                                                                                                        <  2:1905308/148‑1 (MQ=255)
         ttGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTaa                                                                                                                                       >  2:1475520/1‑148 (MQ=255)
         ttGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTaa                                                                                                                                       >  1:3020970/1‑148 (MQ=255)
         ttGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTaa                                                                                                                                       >  1:2220352/1‑148 (MQ=255)
          tGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTaca                                                                                                                                      >  1:84248/1‑146 (MQ=255)
           gTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAg                                                                                                                                     <  2:1344555/148‑1 (MQ=255)
             cGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGgt                                                                                                                                   <  1:1178909/148‑1 (MQ=255)
              ggCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGgtg                                                                                                                                  <  1:443414/148‑1 (MQ=255)
                cGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGtgtt                                                                                                                                >  1:3373375/1‑148 (MQ=255)
                     tgctgGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGa                                                                                                                           <  1:1155902/148‑1 (MQ=255)
                     tgctgGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGa                                                                                                                           <  2:3548917/148‑1 (MQ=255)
                      gctgGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGaa                                                                                                                          >  1:3139554/1‑148 (MQ=255)
                       ctgGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAg                                                                                                                         <  2:2340867/148‑1 (MQ=255)
                           cggcgACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGac                                                                                                                     <  1:1199487/148‑1 (MQ=255)
                           cggcgACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGATAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGac                                                                                                                     <  1:2971473/148‑1 (MQ=255)
                            ggctACACTATTTAATAAAAAAAATGACGTCTACTTTATGGTGGGATTGCTAACGAAATTTGGCTTGTCGGCAAAAAACGCTATTTTTATCGTTGATTTCGCTAAAGATCTCTTGGATAAAGAGGTTAAAGGTGTTGTTGACGCGaca                                                                                                                    <  1:1925400/148‑1 (MQ=255)
                              cgACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACt                                                                                                                  >  2:250500/1‑148 (MQ=255)
                                acacTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGa                                                                                                                >  2:1818271/1‑148 (MQ=255)
                                 cacTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGAt                                                                                                               <  2:370894/148‑1 (MQ=255)
                                 cacTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGAt                                                                                                               >  2:1136135/1‑148 (MQ=255)
                                     ctTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCa                                                                                                           >  2:555639/1‑148 (MQ=255)
                                     ctTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCa                                                                                                           >  2:239498/1‑148 (MQ=255)
                                       ttAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGt                                                                                                         >  1:2037664/1‑148 (MQ=255)
                                          aTCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACg                                                                                                      >  1:1442299/1‑148 (MQ=255)
                                           tCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGt                                                                                                     >  2:3459340/1‑148 (MQ=255)
                                           tCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGt                                                                                                     >  1:1217949/1‑148 (MQ=255)
                                              aaaaaaTGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtg                                                                                                  >  1:2747053/1‑148 (MQ=255)
                                               aaaaaTGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtgc                                                                                                 >  1:1171437/1‑148 (MQ=255)
                                                aaaaTGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtgcg                                                                                                >  2:2368059/1‑148 (MQ=255)
                                                 aaaTGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtgcgt                                                                                               <  2:1802266/148‑1 (MQ=255)
                                                    tGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtgcgtctg                                                                                            >  2:1272811/1‑148 (MQ=255)
                                                    tGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtgcgtctg                                                                                            >  2:1380955/1‑148 (MQ=255)
                                                    tGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtgcgtctg                                                                                            >  1:2321637/1‑148 (MQ=255)
                                                    tGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtgcgtctg                                                                                            >  1:3438827/1‑148 (MQ=255)
                                                           tACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGGCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTa                                                                                     <  2:180940/148‑1 (MQ=255)
                                                            aCTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTAt                                                                                    <  2:1254338/148‑1 (MQ=255)
                                                             cTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATc                                                                                   >  1:2932497/1‑148 (MQ=255)
                                                             cTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATc                                                                                   <  2:1335655/148‑1 (MQ=255)
                                                             cTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATc                                                                                   >  1:3543223/1‑148 (MQ=255)
                                                              ttttTGGTGGGCTTGCTAACGACAATAGGCTTGTCGGCAAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATcc                                                                                  <  1:3302362/148‑1 (MQ=255)
                                                              tttATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATcc                                                                                  >  2:3316478/1‑148 (MQ=255)
                                                                 aTGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCtga                                                                               <  2:2134866/148‑1 (MQ=255)
                                                                 aTGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCtga                                                                               <  2:780819/148‑1 (MQ=255)
                                                                  tggtggGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCtgat                                                                              >  1:1677120/1‑148 (MQ=255)
                                                                    gtggGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCtgatga                                                                            >  2:3093707/1‑148 (MQ=255)
                                                                     tggGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGAc                                                                           <  1:1777136/148‑1 (MQ=255)
                                                                      gggCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGAcc                                                                          <  2:1866145/148‑1 (MQ=255)
                                                                      gggCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGAcc                                                                          <  2:3226201/148‑1 (MQ=255)
                                                                      gggCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGAcc                                                                          <  2:524791/148‑1 (MQ=255)
                                                                       gggttGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACct                                                                         >  2:3342996/4‑148 (MQ=255)
                                                                       ggCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACct                                                                         >  1:3460012/1‑148 (MQ=255)
                                                                       ggCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACct                                                                         >  1:1700925/1‑148 (MQ=255)
                                                                        gCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACctc                                                                        <  1:3476903/148‑1 (MQ=255)
                                                                        gCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACctc                                                                        <  2:2578189/148‑1 (MQ=255)
                                                                        gCTTGCAAACGACAAATGCCTTGTCGGCCAAAAACGCTATTATGATAGATTAGTTCGCGAAAGATCTCATGGATAAATAGTGTAAAGGTGAGGTTGAAGCGACACTGATCGCACTACGTATGCGTCTGCGTCCTATCCTGATGACctc                                                                        >  2:2116045/1‑148 (MQ=255)
                                                                         cTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACctct                                                                       >  2:2908740/1‑148 (MQ=255)
                                                                         cTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACctct                                                                       <  1:583076/148‑1 (MQ=255)
                                                                             cTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCg                                                                   <  2:2983275/148‑1 (MQ=255)
                                                                                  gACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCttt                                                              <  1:1469313/148‑1 (MQ=255)
                                                                                    cAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTAt                                                            >  2:2957917/1‑148 (MQ=255)
                                                                                       ttGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATtct                                                         >  1:140075/1‑148 (MQ=255)
                                                                                          gCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCgg                                                      <  2:2015721/148‑1 (MQ=255)
                                                                                                  gCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTAc                                              >  1:724135/1‑148 (MQ=255)
                                                                                                    cAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCg                                            <  2:2691692/148‑1 (MQ=255)
                                                                                                    cAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCg                                            <  1:1475520/148‑1 (MQ=255)
                                                                                                       aaaCGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCgcta                                         >  1:749068/1‑148 (MQ=255)
                                                                                                       aaaCGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCgcta                                         <  1:2453032/148‑1 (MQ=255)
                                                                                                        aaCGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCgctag                                        <  1:537855/148‑1 (MQ=255)
                                                                                                         aCGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTCTCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCgctagc                                       >  2:377132/1‑148 (MQ=255)
                                                                                                         aCGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGTCTTTATTCTCGGCGTATTACCgctagc                                       <  1:2128288/148‑1 (MQ=255)
                                                                                                         aCGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCgctagc                                       <  2:3373375/148‑1 (MQ=255)
                                                                                                            cTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTAt                                    >  1:139820/1‑148 (MQ=255)
                                                                                                            cTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATAACCGCTAGCTAt                                    >  1:139879/1‑148 (MQ=255)
                                                                                                               ttttGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAg                                 >  1:1120224/1‑148 (MQ=255)
                                                                                                               ttttGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAg                                 >  1:2780841/1‑148 (MQ=255)
                                                                                                                 ttGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTa                               <  2:634286/148‑1 (MQ=255)
                                                                                                                  tGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTaa                              <  2:2874605/148‑1 (MQ=255)
                                                                                                                    aTCGTTGAGTTCGCTAAAGATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTCTCAGTAACg                            >  1:935375/1‑148 (MQ=255)
                                                                                                                      cGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGt                          <  2:531476/148‑1 (MQ=255)
                                                                                                                      cGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGt                          >  1:3350096/1‑148 (MQ=255)
                                                                                                                         tGAGTTCTCTAAAGATAACATGGATCAAGATGGTAAAGGTGTAGTTGAAGCTAAACTGATGGCAGTACGTATTCGTCTGCGTCCTATACTGATCACCTCTCTCGCCTTTATTCTCTGCGTAATACCGCCCGGTATCAGTACCgtcggc                       >  2:2815662/1‑143 (MQ=255)
                                                                                                                         tGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTCGCTATCAGTAACGGTGcc                       >  1:2886216/1‑148 (MQ=255)
                                                                                                                         tGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGcc                       >  2:710938/1‑148 (MQ=255)
                                                                                                                            gTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGc                    >  2:3500269/1‑148 (MQ=255)
                                                                                                                             ttCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCa                   >  1:1752676/1‑148 (MQ=255)
                                                                                                                                 cTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTgg               >  1:1002304/1‑148 (MQ=255)
                                                                                                                                     aGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGgcgcg           >  1:3238971/1‑148 (MQ=255)
                                                                                                                                     aGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGgcgcg           >  1:1346037/1‑148 (MQ=255)
                                                                                                                                      gATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGgcgcgc          >  2:1713772/1‑148 (MQ=255)
                                                                                                                                      gATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGgcgcgc          >  2:227807/1‑148 (MQ=255)
                                                                                                                                          tcATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGaa      <  2:1992180/148‑1 (MQ=255)
                                                                                                                                          tcATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGaa      <  2:2480179/148‑1 (MQ=255)
                                                                                                                                              ggATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCt  >  2:1244624/1‑148 (MQ=255)
                                                                                                                                              ggATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCt  <  2:2220352/148‑1 (MQ=255)
                                                                                                                                              ggATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCt  <  2:3020970/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCT  >  CP009273/3411105‑3411394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGCCGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGG  >  CP009273/3411101‑3411398
                                                                                                                                                     |                                                                                                                                                    
GTGCCGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGAT                                                                                                                                                      <  SRR19973067.3144675/150‑1 (MQ=60)
    CGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAG                                                                                                                                                  <  SRR19973067.2310269/150‑1 (MQ=60)
    CGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAG                                                                                                                                                  <  SRR19973067.1180268/150‑1 (MQ=60)
        AGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGG                                                                                                                                              >  SRR19973067.1244398/1‑150 (MQ=60)
        AGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAAACAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGG                                                                                                                                              >  SRR19973067.1244406/1‑150 (MQ=60)
           GATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAA                                                                                                                                           >  SRR19973067.3040204/1‑150 (MQ=60)
           GATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAA                                                                                                                                           <  SRR19973067.2380363/150‑1 (MQ=60)
           GATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAA                                                                                                                                           <  SRR19973067.251435/150‑1 (MQ=60)
           GATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAA                                                                                                                                           >  SRR19973067.2884320/1‑150 (MQ=60)
           GATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAA                                                                                                                                           >  SRR19973067.638541/1‑150 (MQ=60)
           GATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAA                                                                                                                                           >  SRR19973067.638564/1‑150 (MQ=60)
           GATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAA                                                                                                                                           <  SRR19973067.3536639/150‑1 (MQ=60)
           GATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAA                                                                                                                                           >  SRR19973067.2235132/1‑150 (MQ=60)
           GATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAA                                                                                                                                           <  SRR19973067.582478/150‑1 (MQ=60)
            ATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTACA                                                                                                                                          >  SRR19973067.85166/1‑150 (MQ=60)
             TTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAG                                                                                                                                         <  SRR19973067.767926/150‑1 (MQ=60)
               GTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGT                                                                                                                                       >  SRR19973067.148564/1‑150 (MQ=60)
               GTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGT                                                                                                                                       >  SRR19973067.1649002/1‑150 (MQ=60)
                 CGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGT                                                                                                                                     <  SRR19973067.1187298/150‑1 (MQ=60)
                 CGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGT                                                                                                                                     <  SRR19973067.1186698/150‑1 (MQ=60)
                  GGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTT                                                                                                                                    >  SRR19973067.3394441/1‑150 (MQ=60)
                  GGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTT                                                                                                                                    <  SRR19973067.446487/150‑1 (MQ=60)
                  GGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTT                                                                                                                                    <  SRR19973067.446489/150‑1 (MQ=60)
                  GGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTT                                                                                                                                    >  SRR19973067.1783201/1‑150 (MQ=60)
                  GGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTT                                                                                                                                    >  SRR19973067.3394811/1‑150 (MQ=60)
                        CTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAA                                                                                                                              >  SRR19973067.750998/1‑150 (MQ=60)
                        CTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAA                                                                                                                              >  SRR19973067.3159489/1‑150 (MQ=60)
                         TGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAG                                                                                                                             <  SRR19973067.2366941/150‑1 (MQ=60)
                         TGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAG                                                                                                                             <  SRR19973067.1163615/150‑1 (MQ=60)
                         TGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAG                                                                                                                             <  SRR19973067.1160443/150‑1 (MQ=60)
                               CGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACAC                                                                                                                       <  SRR19973067.1207335/150‑1 (MQ=60)
                               CGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGATAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACAC                                                                                                                       <  SRR19973067.2990218/150‑1 (MQ=60)
                                GGCTACACTATTTAATAAAAAAAATGACGTCTACTTTATGGTGGGATTGCTAACGAAATTTGGCTTGTCGGCAAAAAACGCTATTTTTATCGTTGATTTCGCTAAAGATCTCTTGGATAAAGAGGTTAAAGGTGTTGTTGACGCGACACT                                                                                                                      <  SRR19973067.1938537/150‑1 (MQ=60)
                                       CTATTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGTGTTCGCTAAAGATCTCTTGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCA                                                                                                               <  SRR19973067.939259/150‑1 (MQ=60)
                                         CTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGT                                                                                                             >  SRR19973067.2051303/1‑150 (MQ=60)
                                            TAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACG                                                                                                          >  SRR19973067.1451995/1‑150 (MQ=60)
                                             AATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGT                                                                                                         >  SRR19973067.1226036/1‑150 (MQ=60)
                                                CAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATG                                                                                                      >  SRR19973067.2764804/1‑150 (MQ=60)
                                                CAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATG                                                                                                      >  SRR19973067.144752/1‑150 (MQ=60)
                                                CAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATG                                                                                                      >  SRR19973067.145658/1‑150 (MQ=60)
                                                 AAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGC                                                                                                     >  SRR19973067.1179203/1‑150 (MQ=60)
                                                 AAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCAAGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGC                                                                                                     >  SRR19973067.1179138/1‑150 (MQ=60)
                                                      AATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTG                                                                                                >  SRR19973067.3460721/1‑150 (MQ=60)
                                                      AATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTG                                                                                                >  SRR19973067.2336899/1‑150 (MQ=60)
                                                      AATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTGGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTG                                                                                                <  SRR19973067.3146542/150‑1 (MQ=60)
                                                             TCTACTTTATGGTGGGCTTGCAAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTA                                                                                         <  SRR19973067.2159739/150‑1 (MQ=60)
                                                               TACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATC                                                                                       >  SRR19973067.3565587/1‑150 (MQ=60)
                                                               TACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATC                                                                                       >  SRR19973067.2951116/1‑150 (MQ=60)
                                                                 CTTTATGGTGGGCTTGCTAACGACATTTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGTATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCT                                                                                     <  SRR19973067.343088/150‑1 (MQ=60)
                                                                  TTTTTGGTGGGCTTGCTAACGACAATAGGCTTGTCGGCAAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTG                                                                                    <  SRR19973067.3323179/150‑1 (MQ=60)
                                                                    TATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGAT                                                                                  >  SRR19973067.1688680/1‑150 (MQ=60)
                                                                         TGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCT                                                                             <  SRR19973067.1789170/150‑1 (MQ=60)
                                                                         TGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCT                                                                             >  SRR19973067.1712615/1‑150 (MQ=60)
                                                                         TGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCT                                                                             >  SRR19973067.3482009/1‑150 (MQ=60)
                                                                          GGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTC                                                                            <  SRR19973067.145374/150‑1 (MQ=60)
                                                                           GGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCT                                                                           >  SRR19973067.3323621/1‑150 (MQ=60)
                                                                           GGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCT                                                                           <  SRR19973067.3260761/150‑1 (MQ=60)
                                                                           GGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCT                                                                           <  SRR19973067.3261088/150‑1 (MQ=60)
                                                                            GCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTC                                                                          <  SRR19973067.3498979/150‑1 (MQ=60)
                                                                             CTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCT                                                                         <  SRR19973067.586730/150‑1 (MQ=60)
                                                                             CTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCT                                                                         <  SRR19973067.586813/150‑1 (MQ=60)
                                                                                      GACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTAT                                                                <  SRR19973067.1479106/150‑1 (MQ=60)
                                                                                         AATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCT                                                             >  SRR19973067.141297/1‑150 (MQ=60)
                                                                                         AATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCT                                                             >  SRR19973067.141316/1‑150 (MQ=60)
                                                                                             GGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGC                                                         >  SRR19973067.1700357/1‑150 (MQ=60)
                                                                                             GGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTATTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGC                                                         >  SRR19973067.3469096/1‑150 (MQ=60)
                                                                                                    CGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTAC                                                  >  SRR19973067.729169/1‑150 (MQ=60)
                                                                                                        CAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCT                                              <  SRR19973067.1485336/150‑1 (MQ=60)
                                                                                                         AAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTA                                             >  SRR19973067.754263/1‑150 (MQ=60)
                                                                                                           AAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGC                                           <  SRR19973067.2469157/150‑1 (MQ=60)
                                                                                                           AAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGC                                           <  SRR19973067.2469111/150‑1 (MQ=60)
                                                                                                            AACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCT                                          <  SRR19973067.541446/150‑1 (MQ=60)
                                                                                                             ACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGTCTTTATTCTCGGCGTATTACCGCTAGCTA                                         <  SRR19973067.2142493/150‑1 (MQ=60)
                                                                                                              CGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTAT                                        >  SRR19973067.1121499/1‑150 (MQ=60)
                                                                                                              CGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTAT                                        >  SRR19973067.140684/1‑150 (MQ=60)
                                                                                                              CGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTAT                                        >  SRR19973067.141060/1‑150 (MQ=60)
                                                                                                              CGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTAT                                        >  SRR19973067.2308155/1‑150 (MQ=60)
                                                                                                              CGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATAACCGCTAGCTAT                                        >  SRR19973067.141100/1‑150 (MQ=60)
                                                                                                              CGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATAACCGCTAGCTAT                                        >  SRR19973067.141120/1‑150 (MQ=60)
                                                                                                                CTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCA                                      >  SRR19973067.3489289/1‑150 (MQ=60)
                                                                                                                CTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCA                                      >  SRR19973067.1720043/1‑150 (MQ=60)
                                                                                                                 TATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTCTCAG                                     >  SRR19973067.2798306/1‑150 (MQ=60)
                                                                                                                 TATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAG                                     >  SRR19973067.1127801/1‑150 (MQ=60)
                                                                                                                 TATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAG                                     >  SRR19973067.2798792/1‑150 (MQ=60)
                                                                                                                  ATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGT                                    <  SRR19973067.2088326/150‑1 (MQ=60)
                                                                                                                      TGATCGTTGAGTTCGCTAAAGATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTCTCAGTAACG                                >  SRR19973067.941721/1‑150 (MQ=60)
                                                                                                                        ATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGT                              >  SRR19973067.3371338/1‑150 (MQ=60)
                                                                                                                          CGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGC                            >  SRR19973067.1209062/1‑150 (MQ=60)
                                                                                                                           GTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTCGCTATCAGTAACGGTGCC                           >  SRR19973067.2904669/1‑150 (MQ=60)
                                                                                                                           GTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCC                           >  SRR19973067.1932387/1‑150 (MQ=60)
                                                                                                                           GTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCC                           >  SRR19973067.2029658/1‑150 (MQ=60)
                                                                                                                           GTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCC                           >  SRR19973067.2904960/1‑150 (MQ=60)
                                                                                                                               AGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCA                       >  SRR19973067.1764619/1‑150 (MQ=60)
                                                                                                                               AGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCA                       >  SRR19973067.3011682/1‑150 (MQ=60)
                                                                                                                                   CGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGG                   >  SRR19973067.509526/1‑150 (MQ=60)
                                                                                                                                   CGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGG                   >  SRR19973067.1009192/1‑150 (MQ=60)
                                                                                                                                       AAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCG               >  SRR19973067.1355244/1‑150 (MQ=60)
                                                                                                                                       AAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCG               >  SRR19973067.3259283/1‑150 (MQ=60)
                                                                                                                                             CTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAAC         >  SRR19973067.561014/1‑150 (MQ=60)
                                                                                                                                             CTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAAC         <  SRR19973067.296321/150‑1 (MQ=60)
                                                                                                                                              TCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTCACGGTGCCGGCAGTGGCGCGCAGAACG        >  SRR19973067.3196857/1‑150 (MQ=60)
                                                                                                                                                 TGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTG     <  SRR19973067.1264513/150‑1 (MQ=60)
                                                                                                                                                    ATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGG  >  SRR19973067.1282563/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GTGCCGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGG  >  CP009273/3411101‑3411398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑