Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,411,250 G→T 100% E953D (GAG→GAT)  acrF → multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,411,2500GT97.8% 746.7 / 5.7 247E953D (GAG→GAT) acrFmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (3/3);  new base T (144/97);  total (147/100)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 250 total aligned reads displayed.

CGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTG  >  CP009273/3411105‑3411397
                                                                                                                                                 |                                                                                                                                                   
 gTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATaaa                                                                                                                                                  >  1:1191976/1‑148 (MQ=255)
  ttAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAag                                                                                                                                                 >  1:927074/1‑148 (MQ=255)
   tAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAaga                                                                                                                                                >  2:1959529/1‑148 (MQ=255)
     gggATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAgg                                                                                                                                              >  2:296575/1‑148 (MQ=255)
           gTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAg                                                                                                                                        <  2:468426/148‑1 (MQ=255)
             cGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGgt                                                                                                                                      >  1:2511432/1‑148 (MQ=255)
               gCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGgtgt                                                                                                                                    <  2:541498/148‑1 (MQ=255)
               gCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGgtgt                                                                                                                                    <  2:1537090/148‑1 (MQ=255)
                 gTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGtgttg                                                                                                                                  <  2:3125004/148‑1 (MQ=255)
                 gTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGtgttg                                                                                                                                  <  2:1586882/148‑1 (MQ=255)
                 gTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGtgttg                                                                                                                                  <  2:1245224/148‑1 (MQ=255)
                 gTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGtgttg                                                                                                                                  <  2:1208878/148‑1 (MQ=255)
                       ctgGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAg                                                                                                                            <  2:1996560/148‑1 (MQ=255)
                                acacTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGa                                                                                                                   <  1:143200/148‑1 (MQ=255)
                                acacTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGa                                                                                                                   >  2:3609012/1‑148 (MQ=255)
                                acacTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGa                                                                                                                   >  2:2996247/1‑148 (MQ=255)
                                acacTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGa                                                                                                                   >  2:141668/1‑148 (MQ=255)
                                acacTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGa                                                                                                                   >  1:1695718/1‑148 (MQ=255)
                                  acTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATg                                                                                                                 <  1:2314234/148‑1 (MQ=255)
                                   ctctTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATgg                                                                                                                >  2:2916451/1‑148 (MQ=255)
                                   ctctTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATgg                                                                                                                >  2:1967710/1‑148 (MQ=255)
                                   ctctTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATgg                                                                                                                >  1:3242133/1‑148 (MQ=255)
                                   ctctTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATgg                                                                                                                >  1:1906453/1‑148 (MQ=255)
                                     ctTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTTTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCa                                                                                                              >  1:3755241/1‑148 (MQ=255)
                                     ctTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCa                                                                                                              <  2:559989/148‑1 (MQ=255)
                                     ctTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCa                                                                                                              >  1:3317317/1‑148 (MQ=255)
                                        tAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTa                                                                                                           >  2:279702/1‑148 (MQ=255)
                                           tCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGt                                                                                                        <  2:843049/148‑1 (MQ=255)
                                              aaaaaaTGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtg                                                                                                     >  1:2770835/1‑148 (MQ=255)
                                              aaaaaaTGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtg                                                                                                     >  2:2049764/1‑148 (MQ=255)
                                                 aaaTGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtgcgt                                                                                                  >  1:1773179/1‑148 (MQ=255)
                                                  aaTGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtgcgtc                                                                                                 >  2:811123/1‑148 (MQ=255)
                                                  aaTGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtgcgtc                                                                                                 >  2:1815101/1‑148 (MQ=255)
                                                   aTGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtgcgtct                                                                                                >  1:3112626/1‑148 (MQ=255)
                                                       cGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtgcgtctgcgt                                                                                            >  2:3218776/1‑148 (MQ=255)
                                                         tCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTcc                                                                                          <  2:3415850/148‑1 (MQ=255)
                                                          cTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCt                                                                                         >  1:1070286/1‑148 (MQ=255)
                                                           tACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTa                                                                                        >  1:569473/1‑148 (MQ=255)
                                                           tACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTa                                                                                        >  1:218941/1‑148 (MQ=255)
                                                             cTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATc                                                                                      <  2:702143/148‑1 (MQ=255)
                                                              tttATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATcc                                                                                     >  1:1216016/1‑148 (MQ=255)
                                                              tttATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATcc                                                                                     >  1:1215349/1‑148 (MQ=255)
                                                              tttATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATcc                                                                                     >  1:2462420/1‑148 (MQ=255)
                                                                tATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCtg                                                                                   <  1:296575/148‑1 (MQ=255)
                                                                 aTGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCtga                                                                                  <  2:3635684/148‑1 (MQ=255)
                                                                      gggCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGAcc                                                                             <  2:3722304/148‑1 (MQ=255)
                                                                       ggCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACct                                                                            <  2:3448762/148‑1 (MQ=255)
                                                                       ggCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACct                                                                            <  2:683375/148‑1 (MQ=255)
                                                                       ggCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACct                                                                            <  1:2722340/148‑1 (MQ=255)
                                                                         cTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACctct                                                                          >  1:3793209/1‑148 (MQ=255)
                                                                         cTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACctct                                                                          <  2:1151623/148‑1 (MQ=255)
                                                                         cTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACctct                                                                          <  1:553850/148‑1 (MQ=255)
                                                                          ttGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACctctc                                                                         <  2:1521355/148‑1 (MQ=255)
                                                                               aaCGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGcc                                                                    >  2:3778741/1‑148 (MQ=255)
                                                                                aCGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCt                                                                   >  1:1299590/1‑148 (MQ=255)
                                                                                   aCAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTa                                                                <  2:2637864/148‑1 (MQ=255)
                                                                                   aCAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTa                                                                <  2:2566552/148‑1 (MQ=255)
                                                                                   aCAATTGGCTGGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTa                                                                <  2:723995/148‑1 (MQ=255)
                                                                                    cAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTGTAt                                                               >  1:3690744/1‑148 (MQ=255)
                                                                                      cttGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATtc                                                             <  2:3092155/147‑1 (MQ=255)
                                                                                      aTTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATtc                                                             >  1:1332377/1‑148 (MQ=255)
                                                                                       ttGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATtct                                                            >  1:362156/1‑148 (MQ=255)
                                                                                       ttGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATtct                                                            >  1:303567/1‑148 (MQ=255)
                                                                                          gCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCgg                                                         >  1:904465/1‑148 (MQ=255)
                                                                                          gCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCgg                                                         <  2:2678198/148‑1 (MQ=255)
                                                                                            ttGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCg                                                       <  2:294013/148‑1 (MQ=255)
                                                                                              gTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTa                                                     <  2:2284278/148‑1 (MQ=255)
                                                                                              gTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTa                                                     <  2:3476037/148‑1 (MQ=255)
                                                                                                 ggCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTa                                                  <  2:1864414/148‑1 (MQ=255)
                                                                                                    cAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCg                                               <  2:1173217/148‑1 (MQ=255)
                                                                                                       aaaCGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCgcta                                            >  1:2867976/1‑148 (MQ=255)
                                                                                                              aTTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCa                                     <  1:678916/148‑1 (MQ=255)
                                                                                                                tttGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGt                                   >  1:839660/1‑148 (MQ=255)
                                                                                                                 ttGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTa                                  <  2:2166715/148‑1 (MQ=255)
                                                                                                                 ttGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTa                                  <  1:598610/148‑1 (MQ=255)
                                                                                                                  tGATCGTTGAGTTCGCTAAAGATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTaa                                 <  2:1632653/148‑1 (MQ=255)
                                                                                                                    aTCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACg                               >  1:826818/1‑148 (MQ=255)
                                                                                                                    aTCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACg                               >  1:3816235/1‑148 (MQ=255)
                                                                                                                    aTCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACg                               <  1:3487580/148‑1 (MQ=255)
                                                                                                                    aTCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACg                               >  1:3370913/1‑148 (MQ=255)
                                                                                                                     tCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACgg                              >  2:889801/1‑148 (MQ=255)
                                                                                                                         tGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGcc                          >  1:2242489/1‑148 (MQ=255)
                                                                                                                             ttCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCa                      >  1:2048996/1‑148 (MQ=255)
                                                                                                                                 cTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTgg                  >  2:2195463/1‑148 (MQ=255)
                                                                                                                                 cTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTgg                  >  1:217557/1‑148 (MQ=255)
                                                                                                                                 cTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTgg                  >  2:659198/1‑148 (MQ=255)
                                                                                                                                  tAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGgc                 >  2:3710885/1‑148 (MQ=255)
                                                                                                                                  tAAAGATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGgc                 >  1:1617906/1‑148 (MQ=255)
                                                                                                                                     aGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTCTCAGTAACGGTGCCGGCAGTGgcgcg              >  2:628676/1‑148 (MQ=255)
                                                                                                                                     aGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGgcgcg              >  2:1346930/1‑148 (MQ=255)
                                                                                                                                      gATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGgcgcgc             >  2:1001721/1‑148 (MQ=255)
                                                                                                                                         ctcATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGa          >  1:945666/1‑148 (MQ=255)
                                                                                                                                           cATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTg                            <  2:1352038/128‑1 (MQ=255)
                                                                                                                                           cATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAAc        >  2:75971/1‑148 (MQ=255)
                                                                                                                                           cATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAAc        >  2:2835451/1‑148 (MQ=255)
                                                                                                                                             tGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGc      <  2:3526504/148‑1 (MQ=255)
                                                                                                                                             tGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGc      >  2:3334214/1‑148 (MQ=255)
                                                                                                                                              ggATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCt     >  2:388787/1‑148 (MQ=255)
                                                                                                                                               gagaAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCtg    <  2:127178/148‑1 (MQ=255)
                                                                                                                                                 gaAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCtgtg  <  1:2603872/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTG  >  CP009273/3411105‑3411397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGG  >  CP009273/3411104‑3411399
                                                                                                                                                  |                                                                                                                                                     
CCGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAA                                                                                                                                                    >  SRR19973069.1200191/1‑150 (MQ=60)
 CGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAG                                                                                                                                                   >  SRR19973069.933375/1‑150 (MQ=60)
      GGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGT                                                                                                                                              >  SRR19973069.3312003/1‑150 (MQ=60)
        GATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAA                                                                                                                                            >  SRR19973069.9036/1‑150 (MQ=60)
            GTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGT                                                                                                                                        >  SRR19973069.596047/1‑150 (MQ=60)
            GTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGT                                                                                                                                        >  SRR19973069.2528166/1‑150 (MQ=60)
             TCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTG                                                                                                                                       >  SRR19973069.1909194/1‑150 (MQ=60)
                GCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTG                                                                                                                                    <  SRR19973069.1659438/150‑1 (MQ=60)
                GCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTG                                                                                                                                    <  SRR19973069.1659354/150‑1 (MQ=60)
                  GTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTT                                                                                                                                  <  SRR19973069.2764652/150‑1 (MQ=60)
                               CGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGA                                                                                                                     >  SRR19973069.3803913/1‑150 (MQ=60)
                               CGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGA                                                                                                                     >  SRR19973069.1707824/1‑150 (MQ=60)
                               CGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGA                                                                                                                     >  SRR19973069.1707568/1‑150 (MQ=60)
                                 ACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATG                                                                                                                   <  SRR19973069.144495/150‑1 (MQ=60)
                                 ACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCAAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTTTTGAAGCGACACTGATG                                                                                                                   <  SRR19973069.3645782/150‑1 (MQ=60)
                                 ACAATATTTAATCAAAAAAATGACGTCTACTTTATGGGGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTTATCGTTGATTTCGCTAAAGATCTCATGGATAAAGAGGTTAAAGGTGTTGTTGAAGCGACACTGATG                                                                                                                   <  SRR19973069.143780/150‑1 (MQ=60)
                                  CACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGG                                                                                                                  >  SRR19973069.1919618/1‑150 (MQ=60)
                                  CACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGG                                                                                                                  >  SRR19973069.3263354/1‑150 (MQ=60)
                                   ACTCTTTAATCAAAAAAATGACGTCTACTTTTTGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGC                                                                                                                 <  SRR19973069.2329986/150‑1 (MQ=60)
                                   ACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGC                                                                                                                 >  SRR19973069.2452180/1‑150 (MQ=60)
                                   ACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGC                                                                                                                 <  SRR19973069.934794/150‑1 (MQ=60)
                                   ACTCTTAAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGC                                                                                                                 <  SRR19973069.934985/150‑1 (MQ=60)
                                    CTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTTTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCA                                                                                                                >  SRR19973069.3779671/1‑150 (MQ=60)
                                    CTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCA                                                                                                                >  SRR19973069.3339063/1‑150 (MQ=60)
                                          AATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGT                                                                                                          >  SRR19973069.2937003/1‑150 (MQ=60)
                                           ATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTA                                                                                                         >  SRR19973069.1205226/1‑150 (MQ=60)
                                           ATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGATATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTA                                                                                                         <  SRR19973069.1326842/150‑1 (MQ=60)
                                             CAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATG                                                                                                       >  SRR19973069.3746600/1‑150 (MQ=60)
                                             CAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATG                                                                                                       >  SRR19973069.2789294/1‑150 (MQ=60)
                                              AAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGC                                                                                                      <  SRR19973069.824912/150‑1 (MQ=60)
                                                AAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGT                                                                                                    >  SRR19973069.1785670/1‑150 (MQ=60)
                                                AAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGT                                                                                                    >  SRR19973069.688128/1‑150 (MQ=60)
                                                  AAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCT                                                                                                  >  SRR19973069.3133095/1‑150 (MQ=60)
                                                         GTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCT                                                                                           <  SRR19973069.2842946/150‑1 (MQ=60)
                                                         GTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCT                                                                                           >  SRR19973069.3478709/1‑150 (MQ=60)
                                                         GTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCT                                                                                           >  SRR19973069.1077748/1‑150 (MQ=60)
                                                          TCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTA                                                                                          >  SRR19973069.220690/1‑150 (MQ=60)
                                                          TCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTA                                                                                          >  SRR19973069.220647/1‑150 (MQ=60)
                                                          TCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTA                                                                                          >  SRR19973069.573420/1‑150 (MQ=60)
                                                             ACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCC                                                                                       >  SRR19973069.1223676/1‑150 (MQ=60)
                                                             ACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCC                                                                                       >  SRR19973069.1223754/1‑150 (MQ=60)
                                                             ACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCC                                                                                       >  SRR19973069.1224346/1‑150 (MQ=60)
                                                             ACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCC                                                                                       >  SRR19973069.2479232/1‑150 (MQ=60)
                                                             ACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCC                                                                                       >  SRR19973069.2478953/1‑150 (MQ=60)
                                                                 TTTGGTGGGCTGGCTACCGACAATTGGCTTGTCGTCAAAAAACGCTTTTTTGATCGTTGATTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGAT                                                                                   <  SRR19973069.2639581/150‑1 (MQ=60)
                                                                 TATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGAT                                                                                   <  SRR19973069.298598/150‑1 (MQ=60)
                                                                      TGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCT                                                                              <  SRR19973069.118343/150‑1 (MQ=60)
                                                                       GGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTC                                                                             >  SRR19973069.491215/1‑150 (MQ=60)
                                                                        GGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCT                                                                            >  SRR19973069.3817800/1‑150 (MQ=60)
                                                                        GGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCT                                                                            >  SRR19973069.3817796/1‑150 (MQ=60)
                                                                        GGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCT                                                                            <  SRR19973069.2740580/150‑1 (MQ=60)
                                                                          CTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCT                                                                          <  SRR19973069.557712/150‑1 (MQ=60)
                                                                          CTTGATAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCT                                                                          <  SRR19973069.2972/150‑1 (MQ=60)
                                                                             GCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGC                                                                       >  SRR19973069.2787805/1‑150 (MQ=60)
                                                                              CTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCC                                                                      >  SRR19973069.666495/1‑150 (MQ=60)
                                                                               TAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCT                                                                     >  SRR19973069.1308376/1‑150 (MQ=60)
                                                                                   GACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTGTAT                                                                 >  SRR19973069.3714880/1‑150 (MQ=60)
                                                                                     CAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTC                                                               >  SRR19973069.1341558/1‑150 (MQ=60)
                                                                                      AATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCT                                                              >  SRR19973069.305651/1‑150 (MQ=60)
                                                                                      AATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCT                                                              >  SRR19973069.305619/1‑150 (MQ=60)
                                                                                      AATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCT                                                              >  SRR19973069.364766/1‑150 (MQ=60)
                                                                                         TGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGG                                                           >  SRR19973069.1239680/1‑150 (MQ=60)
                                                                                         TGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGG                                                           <  SRR19973069.2376126/150‑1 (MQ=60)
                                                                                         TGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGG                                                           >  SRR19973069.910672/1‑150 (MQ=60)
                                                                                          GGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGNC                                                          <  SRR19973069.910555/150‑1 (MQ=60)
                                                                                          GGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGC                                                          <  SRR19973069.1852394/150‑1 (MQ=60)
                                                                                          GGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGC                                                          <  SRR19973069.910714/150‑1 (MQ=60)
                                                                                               GTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATT                                                     <  SRR19973069.3428182/150‑1 (MQ=60)
                                                                                                  GGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACC                                                  >  SRR19973069.128372/1‑150 (MQ=60)
                                                                                                      AAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTA                                              >  SRR19973069.2886813/1‑150 (MQ=60)
                                                                                                      AAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTA                                              >  SRR19973069.122613/1‑150 (MQ=60)
                                                                                                      AAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGGTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTA                                              >  SRR19973069.3813327/1‑150 (MQ=60)
                                                                                                       AAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAG                                             >  SRR19973069.3614235/1‑150 (MQ=60)
                                                                                                        AAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGC                                            <  SRR19973069.2063997/150‑1 (MQ=60)
                                                                                                         AACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCT                                           <  SRR19973069.1515241/150‑1 (MQ=60)
                                                                                                           CGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTAT                                         >  SRR19973069.527153/1‑150 (MQ=60)
                                                                                                              TATATTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAG                                      >  SRR19973069.2266247/1‑150 (MQ=60)
                                                                                                               ATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGT                                     >  SRR19973069.845556/1‑150 (MQ=60)
                                                                                                               ATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGT                                     <  SRR19973069.683633/150‑1 (MQ=60)
                                                                                                               ATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGT                                     >  SRR19973069.2066865/1‑150 (MQ=60)
                                                                                                                 TTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAA                                   >  SRR19973069.332106/1‑150 (MQ=60)
                                                                                                                  TTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAAC                                  <  SRR19973069.602649/150‑1 (MQ=60)
                                                                                                                   TGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACG                                 >  SRR19973069.3392954/1‑150 (MQ=60)
                                                                                                                   TGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACG                                 >  SRR19973069.3392035/1‑150 (MQ=60)
                                                                                                                   TGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACG                                 >  SRR19973069.3840935/1‑150 (MQ=60)
                                                                                                                   TGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACG                                 >  SRR19973069.832645/1‑150 (MQ=60)
                                                                                                                     ATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGT                               >  SRR19973069.3653990/1‑150 (MQ=60)
                                                                                                                     ATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGT                               <  SRR19973069.598725/150‑1 (MQ=60)
                                                                                                                     ATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGT                               <  SRR19973069.3510081/150‑1 (MQ=60)
                                                                                                                        GTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCC                            >  SRR19973069.2257417/1‑150 (MQ=60)
                                                                                                                        GTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCC                            >  SRR19973069.835460/1‑150 (MQ=60)
                                                                                                                        GTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCC                            >  SRR19973069.2257595/1‑150 (MQ=60)
                                                                                                                            AGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCA                        >  SRR19973069.2063219/1‑150 (MQ=60)
                                                                                                                                CGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGG                    >  SRR19973069.2598380/1‑150 (MQ=60)
                                                                                                                                CGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGG                    >  SRR19973069.219255/1‑150 (MQ=60)
                                                                                                                                CGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGG                    >  SRR19973069.219306/1‑150 (MQ=60)
                                                                                                                                 GCTAAAGATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGC                   >  SRR19973069.1628847/1‑150 (MQ=60)
                                                                                                                                     AAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGC               >  SRR19973069.3126020/1‑150 (MQ=60)
                                                                                                                                      AGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCA              <  SRR19973069.569103/150‑1 (MQ=60)
                                                                                                                                        ATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGA            >  SRR19973069.952038/1‑150 (MQ=60)
                                                                                                                                        ATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGA            >  SRR19973069.2020259/1‑150 (MQ=60)
                                                                                                                                          CTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCagatcggaagagcacacg          >  SRR19973069.2995955/1‑132 (MQ=60)
                                                                                                                                          CTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCagatcggaagagcacacg          >  SRR19973069.1361407/1‑132 (MQ=60)
                                                                                                                                          CTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAAC          >  SRR19973069.3199796/1‑150 (MQ=60)
                                                                                                                                              TGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTG      >  SRR19973069.2046848/1‑150 (MQ=60)
                                                                                                                                               GGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGT     >  SRR19973069.2331674/1‑150 (MQ=60)
                                                                                                                                                GATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTG    >  SRR19973069.42979/1‑150 (MQ=60)
                                                                                                                                                GATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTG    <  SRR19973069.1973109/150‑1 (MQ=60)
                                                                                                                                                  TAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCGATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGG  <  SRR19973069.1080920/150‑1 (MQ=60)
                                                                                                                                                  GAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGG  <  SRR19973069.2621255/150‑1 (MQ=60)
                                                                                                                                                  GAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGG  <  SRR19973069.2621053/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
CCGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGG  >  CP009273/3411104‑3411399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑