Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,411,250 G→T 100% E953D (GAG→GAT)  acrF → multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,411,2500GT98.9% 590.3 / ‑1.9 188E953D (GAG→GAT) acrFmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (97/89);  total (98/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 188 total aligned reads displayed.

CGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACG  >  CP009273/3411105‑3411392
                                                                                                                                                 |                                                                                                                                              
cGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATaa                                                                                                                                              <  1:3266308/148‑1 (MQ=255)
  ttAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAag                                                                                                                                            >  1:381745/1‑148 (MQ=255)
  ttAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAag                                                                                                                                            >  1:2049578/1‑148 (MQ=255)
     gggATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAgg                                                                                                                                         >  2:204485/1‑148 (MQ=255)
      ggATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAggg                                                                                                                                        >  1:3242757/1‑148 (MQ=255)
      ggATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAggg                                                                                                                                        >  1:1885204/1‑148 (MQ=255)
         ttGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTaa                                                                                                                                     <  2:3042029/148‑1 (MQ=255)
         ttGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTaa                                                                                                                                     <  2:55764/148‑1 (MQ=255)
            tCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAgg                                                                                                                                  <  2:3514873/148‑1 (MQ=255)
            tCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAgg                                                                                                                                  <  2:1751425/148‑1 (MQ=255)
             cGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCGACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGAGAAAGAGGGTAAAGgt                                                                                                                                 <  2:2513598/148‑1 (MQ=255)
               gCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAAAGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTACAGgtgt                                                                                                                               >  2:1131627/1‑148 (MQ=255)
                  tgctgcTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGtgttgt                                                                                                                            <  1:2040668/148‑1 (MQ=255)
                        tgGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGc                                                                                                                      <  2:1347095/148‑1 (MQ=255)
                          gcggcgACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGa                                                                                                                    <  2:2734471/148‑1 (MQ=255)
                             gcgACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGacac                                                                                                                 >  1:739043/1‑148 (MQ=255)
                               gACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTg                                                                                                               <  2:2207588/148‑1 (MQ=255)
                                aaacTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGa                                                                                                              <  1:2818833/146‑1 (MQ=255)
                                 cacTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGAt                                                                                                             >  2:136224/1‑148 (MQ=255)
                                 cacTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGAt                                                                                                             <  2:3391101/148‑1 (MQ=255)
                                   ctctTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATgg                                                                                                           >  2:2956051/1‑148 (MQ=255)
                                    tctTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGc                                                                                                          >  1:1843563/1‑148 (MQ=255)
                                     ctTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCa                                                                                                         >  2:1056232/1‑148 (MQ=255)
                                       ttAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGt                                                                                                       >  1:1537723/1‑148 (MQ=255)
                                          aTCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACg                                                                                                    >  2:1851131/1‑148 (MQ=255)
                                          aTCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACg                                                                                                    >  1:1827973/1‑148 (MQ=255)
                                           tCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGAAAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGt                                                                                                   <  1:111689/148‑1 (MQ=255)
                                            cAAAAAAATGACGTCTACTTTAGGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTa                                                                                                  <  1:2396900/148‑1 (MQ=255)
                                             aaaaaaaTGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAt                                                                                                 >  1:2783638/1‑148 (MQ=255)
                                               aaaaaTGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtgc                                                                                               >  2:827320/1‑148 (MQ=255)
                                                  aaTGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtgcgtc                                                                                            >  2:1625155/1‑148 (MQ=255)
                                                    tGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtgcgtctg                                                                                          >  2:1033934/1‑148 (MQ=255)
                                                    tGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtgcgtctg                                                                                          >  1:32494/1‑148 (MQ=255)
                                                     gACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtgcgtctgc                                                                                         >  1:1244663/1‑148 (MQ=255)
                                                       cGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtgcgtctgcgt                                                                                       <  1:2935459/148‑1 (MQ=255)
                                                         tCTACTTTATGGTGTGATTGCTAACGACTATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTcc                                                                                     <  2:2540049/148‑1 (MQ=255)
                                                          cTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCt                                                                                    <  2:2228282/148‑1 (MQ=255)
                                                            aCTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTAt                                                                                  >  1:2272977/1‑148 (MQ=255)
                                                             cTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATc                                                                                 >  2:1413182/1‑148 (MQ=255)
                                                             cTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATc                                                                                 >  2:3360839/1‑148 (MQ=255)
                                                             cTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATc                                                                                 <  1:807221/148‑1 (MQ=255)
                                                             cTTTAGGGTGGGCTTGCTATCGACAATGGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTTAGTTCGCTAAAGATCTCATGGATAAAGAGTGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATc                                                                                 <  2:2969354/148‑1 (MQ=255)
                                                               ttATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCt                                                                               >  2:3233709/1‑148 (MQ=255)
                                                               ttATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCt                                                                               >  2:3447151/1‑148 (MQ=255)
                                                                tATGGTGGGCTTGCTAACGACAATTGGCTTGTCGCCAAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCtg                                                                              <  1:3347956/148‑1 (MQ=255)
                                                                  tggtggGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCtgat                                                                            <  1:1649791/148‑1 (MQ=255)
                                                                  tggtggGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCtgat                                                                            <  1:1649795/148‑1 (MQ=255)
                                                                   ggtggGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCtgatg                                                                           <  2:1678909/148‑1 (MQ=255)
                                                                    gtggGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCtgatga                                                                          >  1:3211174/1‑148 (MQ=255)
                                                                    gtggGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCtgatga                                                                          >  2:826399/1‑148 (MQ=255)
                                                                     tggGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGAc                                                                         <  1:3445436/148‑1 (MQ=255)
                                                                      gggCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGAcc                                                                        <  2:2753482/148‑1 (MQ=255)
                                                                       ggCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCCTGGATAAAGAGGGTAAAGGGGTTGGTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACct                                                                       >  2:2395414/1‑148 (MQ=255)
                                                                       ggCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACct                                                                       <  1:2925688/148‑1 (MQ=255)
                                                                          ttGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACctctc                                                                    <  1:1633204/148‑1 (MQ=255)
                                                                              tAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGc                                                                <  2:2794736/148‑1 (MQ=255)
                                                                              tAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGc                                                                <  2:2500774/148‑1 (MQ=255)
                                                                              tAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGc                                                                <  1:1732041/148‑1 (MQ=255)
                                                                              tAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGAGGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGc                                                                <  1:428751/148‑1 (MQ=255)
                                                                                 cGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCtt                                                             >  2:3165186/1‑148 (MQ=255)
                                                                                  gACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCttt                                                            >  1:2352381/1‑148 (MQ=255)
                                                                                    cAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTAt                                                          >  1:1427789/1‑148 (MQ=255)
                                                                                       ttGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATtct                                                       >  2:2538609/1‑148 (MQ=255)
                                                                                        tGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATtctc                                                      >  2:226509/1‑148 (MQ=255)
                                                                                            ttGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCg                                                  <  2:2165545/148‑1 (MQ=255)
                                                                                             tGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGt                                                 >  1:2850273/1‑148 (MQ=255)
                                                                                                 ggCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTa                                             <  1:1683254/148‑1 (MQ=255)
                                                                                                 ggCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTa                                             <  1:2818288/148‑1 (MQ=255)
                                                                                                   ccAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTAcc                                           >  2:184560/1‑148 (MQ=255)
                                                                                                    cAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCg                                          <  2:1300369/148‑1 (MQ=255)
                                                                                                    cAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCg                                          <  1:2563968/148‑1 (MQ=255)
                                                                                                    cAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCg                                          <  1:2868874/148‑1 (MQ=255)
                                                                                                    cAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCg                                          <  1:3245604/148‑1 (MQ=255)
                                                                                                     aaaaaCGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCgc                                         <  2:1293685/148‑1 (MQ=255)
                                                                                                            cTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTCGCTAt                                  >  2:2983930/1‑148 (MQ=255)
                                                                                                             tATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATc                                 >  1:68078/1‑148 (MQ=255)
                                                                                                              aTTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCa                                <  1:3509014/148‑1 (MQ=255)
                                                                                                               ttttGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCACTCGCCTTTATTCTCGGCGTATTACCGCTCGCTCTCAg                               >  2:2149952/1‑148 (MQ=255)
                                                                                                                tttGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGt                              <  2:1640058/148‑1 (MQ=255)
                                                                                                                  tGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTaa                            >  1:1870977/1‑148 (MQ=255)
                                                                                                                     tCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACgg                         >  1:1089203/1‑148 (MQ=255)
                                                                                                                     tCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACgg                         >  2:432771/1‑148 (MQ=255)
                                                                                                                     tCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACgg                         >  1:3362133/1‑148 (MQ=255)
                                                                                                                      cGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTCTCAGTAACGGt                        >  2:727197/1‑148 (MQ=255)
                                                                                                                        ttGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGc                      >  2:1986517/1‑148 (MQ=255)
                                                                                                                           aGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCgg                   >  2:3367243/1‑148 (MQ=255)
                                                                                                                              tCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAg                <  2:2626707/148‑1 (MQ=255)
                                                                                                                              tCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAg                <  2:2626711/148‑1 (MQ=255)
                                                                                                                                 cTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTgg             >  2:2083083/1‑148 (MQ=255)
                                                                                                                                  tAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCGAGCTATCAGTCACGGTGCCGGCAGTGgc            >  1:1854921/1‑148 (MQ=255)
                                                                                                                                   aaaGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGgcg           >  2:547417/1‑148 (MQ=255)
                                                                                                                                     aGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGgcgcg         >  1:3413259/1‑148 (MQ=255)
                                                                                                                                      gATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGgcgcgc        >  1:1731219/1‑148 (MQ=255)
                                                                                                                                      gATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGgcgcgc        >  2:696653/1‑148 (MQ=255)
                                                                                                                                       aTCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCa       >  1:1056598/1‑148 (MQ=255)
                                                                                                                                         ctcATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGa     <  2:2062174/148‑1 (MQ=255)
                                                                                                                                           cATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAAc   >  2:2841795/1‑148 (MQ=255)
                                                                                                                                            aTGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACg  <  1:590224/148‑1 (MQ=255)
                                                                                                                                            aTGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACg  >  2:3489272/1‑148 (MQ=255)
                                                                                                                                            aTGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACg  >  1:1226019/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
CGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACG  >  CP009273/3411105‑3411392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGCCGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTG  >  CP009273/3411101‑3411397
                                                                                                                                                     |                                                                                                                                                   
GTGCCGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGAT                                                                                                                                                     <  SRR19973065.1775260/150‑1 (MQ=60)
    CGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAG                                                                                                                                                 >  SRR19973065.384415/1‑150 (MQ=60)
    CGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAG                                                                                                                                                 >  SRR19973065.2062674/1‑150 (MQ=60)
    CGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAG                                                                                                                                                 <  SRR19973065.3286111/150‑1 (MQ=60)
     GTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCAAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGA                                                                                                                                                >  SRR19973065.1424895/1‑150 (MQ=60)
       TAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGG                                                                                                                                              >  SRR19973065.2810445/1‑150 (MQ=60)
        AGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGG                                                                                                                                             >  SRR19973065.1897613/1‑150 (MQ=60)
        AGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGG                                                                                                                                             >  SRR19973065.3262305/1‑150 (MQ=60)
          GGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTA                                                                                                                                           <  SRR19973065.1113464/150‑1 (MQ=60)
          GGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTA                                                                                                                                           <  SRR19973065.2875994/150‑1 (MQ=60)
            ATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAA                                                                                                                                         >  SRR19973065.16118/1‑150 (MQ=60)
               GTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGT                                                                                                                                      <  SRR19973065.612623/150‑1 (MQ=60)
                  GGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTT                                                                                                                                   <  SRR19973065.224843/150‑1 (MQ=60)
                      TGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTG                                                                                                                               <  SRR19973065.2053733/150‑1 (MQ=60)
                      TGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTG                                                                                                                               <  SRR19973065.706173/150‑1 (MQ=60)
                        CTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAA                                                                                                                             <  SRR19973065.2296954/150‑1 (MQ=60)
                               CGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACAC                                                                                                                      >  SRR19973065.743987/1‑150 (MQ=60)
                                    ACACTATTTAATAAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCAAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATG                                                                                                                 <  SRR19973065.2836278/150‑1 (MQ=60)
                                    AAACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATG                                                                                                                 <  SRR19973065.2836247/150‑1 (MQ=60)
                                      ACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGC                                                                                                               >  SRR19973065.1855722/1‑150 (MQ=60)
                                         CTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGT                                                                                                            >  SRR19973065.1547869/1‑150 (MQ=60)
                                            TAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACG                                                                                                         >  SRR19973065.1671080/1‑150 (MQ=60)
                                            TAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACG                                                                                                         >  SRR19973065.1840014/1‑150 (MQ=60)
                                               TCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGTTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAT                                                                                                      <  SRR19973065.3324425/150‑1 (MQ=60)
                                               TCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAT                                                                                                      >  SRR19973065.2800909/1‑150 (MQ=60)
                                               TCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAT                                                                                                      <  SRR19973065.563660/150‑1 (MQ=60)
                                               TCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGAAAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAT                                                                                                      <  SRR19973065.112693/150‑1 (MQ=60)
                                                CAAAAAAATGACGTCTACTTTAGGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATG                                                                                                     <  SRR19973065.2411973/150‑1 (MQ=60)
                                                      AATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTG                                                                                               >  SRR19973065.353975/1‑150 (MQ=60)
                                                      AATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTG                                                                                               >  SRR19973065.3441947/1‑150 (MQ=60)
                                                      AATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTG                                                                                               >  SRR19973065.32901/1‑150 (MQ=60)
                                                      AATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTG                                                                                               >  SRR19973065.32852/1‑150 (MQ=60)
                                                       ATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGC                                                                                              >  SRR19973065.1252851/1‑150 (MQ=60)
                                                           CGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCC                                                                                          <  SRR19973065.2953261/150‑1 (MQ=60)
                                                              CTACTTTATGGTGGGCTTGCTACCGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTAT                                                                                       <  SRR19973065.3274994/150‑1 (MQ=60)
                                                              CTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTAT                                                                                       >  SRR19973065.2287327/1‑150 (MQ=60)
                                                                 CTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCT                                                                                    <  SRR19973065.1867794/150‑1 (MQ=60)
                                                                 CTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCT                                                                                    <  SRR19973065.1142080/150‑1 (MQ=60)
                                                                 CTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCT                                                                                    <  SRR19973065.812552/150‑1 (MQ=60)
                                                                    TATGGTGGGCTTGCTAACGACAATTGGCTTGTCGCCAAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGAT                                                                                 <  SRR19973065.3368412/150‑1 (MQ=60)
                                                                      TGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGA                                                                               <  SRR19973065.1660859/150‑1 (MQ=60)
                                                                      TGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGA                                                                               <  SRR19973065.1660863/150‑1 (MQ=60)
                                                                      TGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGA                                                                               <  SRR19973065.3247996/150‑1 (MQ=60)
                                                                      TGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGA                                                                               >  SRR19973065.3230489/1‑150 (MQ=60)
                                                                         TGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTTTTTTGATCGTTGAGTTCGCTAAAGATCTCATGTATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCT                                                                            <  SRR19973065.3466446/150‑1 (MQ=60)
                                                                         TGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCT                                                                            <  SRR19973065.3466441/150‑1 (MQ=60)
                                                                           GGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCT                                                                          <  SRR19973065.2943462/150‑1 (MQ=60)
                                                                           GGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCT                                                                          <  SRR19973065.2943361/150‑1 (MQ=60)
                                                                             CTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCT                                                                        >  SRR19973065.1644966/1‑150 (MQ=60)
                                                                              TTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTC                                                                       <  SRR19973065.1644166/150‑1 (MQ=60)
                                                                                 CTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCC                                                                    >  SRR19973065.2953524/1‑150 (MQ=60)
                                                                                  TAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCT                                                                   <  SRR19973065.1743578/150‑1 (MQ=60)
                                                                                  TAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCT                                                                   <  SRR19973065.1743504/150‑1 (MQ=60)
                                                                                  TAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCT                                                                   <  SRR19973065.1743486/150‑1 (MQ=60)
                                                                                  TAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGAGGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCT                                                                   <  SRR19973065.431727/150‑1 (MQ=60)
                                                                                    ACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTT                                                                 >  SRR19973065.2367020/1‑150 (MQ=60)
                                                                                    ACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTT                                                                 >  SRR19973065.2367037/1‑150 (MQ=60)
                                                                                      GACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTAT                                                               >  SRR19973065.1437085/1‑150 (MQ=60)
                                                                                         AATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCT                                                            <  SRR19973065.2953083/150‑1 (MQ=60)
                                                                                            TGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGG                                                         >  SRR19973065.1499914/1‑150 (MQ=60)
                                                                                               CTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGT                                                      >  SRR19973065.2867803/1‑150 (MQ=60)
                                                                                               CTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGT                                                      >  SRR19973065.1849005/1‑150 (MQ=60)
                                                                                               CTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGT                                                      >  SRR19973065.2060388/1‑150 (MQ=60)
                                                                                                     GGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACC                                                <  SRR19973065.1694514/150‑1 (MQ=60)
                                                                                                     GGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACC                                                <  SRR19973065.2835700/150‑1 (MQ=60)
                                                                                                     GGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACC                                                <  SRR19973065.1694510/150‑1 (MQ=60)
                                                                                                        CAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCT                                             <  SRR19973065.2886470/150‑1 (MQ=60)
                                                                                                        CAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCT                                             <  SRR19973065.2580582/150‑1 (MQ=60)
                                                                                                        CAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCT                                             <  SRR19973065.2579990/150‑1 (MQ=60)
                                                                                                        CAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCT                                             <  SRR19973065.3265191/150‑1 (MQ=60)
                                                                                                        AAAAAACGCTATTTTGATAGTTGAGTTCGCTAAAGTTCTCATTGATAATGAGTTTAATGTTGTTGTTTAAGCGACACTGATGTCAGTACGTATGCGTCTGGGTCCTATCCTGATGAACTCTCTCGCCTTTATTCTCGGCGTATTACCTCT                                             <  SRR19973065.2551456/150‑1 (MQ=60)
                                                                                                          AAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAG                                           <  SRR19973065.965516/150‑1 (MQ=60)
                                                                                                               GCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATC                                      >  SRR19973065.68797/1‑150 (MQ=60)
                                                                                                                 TATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAG                                    <  SRR19973065.791723/150‑1 (MQ=60)
                                                                                                                  ATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGT                                   <  SRR19973065.3530306/150‑1 (MQ=60)
                                                                                                                    TTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAA                                 >  SRR19973065.1883296/1‑150 (MQ=60)
                                                                                                                       GATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGG                              >  SRR19973065.3382690/1‑150 (MQ=60)
                                                                                                                       GATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGG                              >  SRR19973065.1096380/1‑150 (MQ=60)
                                                                                                                       GATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGG                              >  SRR19973065.3382628/1‑150 (MQ=60)
                                                                                                                        ATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGT                             <  SRR19973065.1930641/150‑1 (MQ=60)
                                                                                                                                    GCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGC                 >  SRR19973065.1866969/1‑150 (MQ=60)
                                                                                                                                    GCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCGAGCTATCAGTCACGGTGCCGGCAGTGGC                 >  SRR19973065.1867133/1‑150 (MQ=60)
                                                                                                                                       AAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCG              >  SRR19973065.3434113/1‑150 (MQ=60)
                                                                                                                                       AAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCG              >  SRR19973065.3434110/1‑150 (MQ=60)
                                                                                                                                        AAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGC             >  SRR19973065.1742681/1‑150 (MQ=60)
                                                                                                                                         AGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCA            >  SRR19973065.1063604/1‑150 (MQ=60)
                                                                                                                                              TCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACG       >  SRR19973065.1234018/1‑150 (MQ=60)
                                                                                                                                              TCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTCATGACCTCTCTCGCATTTATTCTCGGCGTATTACCGCTAGCTATCAGTACCGGTGCCGGCAGTGGCGCGCAGAACG       >  SRR19973065.1234019/1‑150 (MQ=60)
                                                                                                                                                ATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCT     <  SRR19973065.593883/150‑1 (MQ=60)
                                                                                                                                                   GATAAAGAGGTTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTG  <  SRR19973065.2978668/150‑1 (MQ=60)
                                                                                                                                                   GATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTG  <  SRR19973065.3184358/150‑1 (MQ=60)
                                                                                                                                                   GATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTG  <  SRR19973065.1138974/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
GTGCCGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTG  >  CP009273/3411101‑3411397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑