Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,411,250 G→T 100% E953D (GAG→GAT)  acrF → multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,411,2500GT95.4% 565.8 / 15.4 197E953D (GAG→GAT) acrFmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (8/2);  new base T (112/75);  total (120/77)
Fisher's exact test for biased strand distribution p-value = 3.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 197 total aligned reads displayed.

GTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCT  >  CP009273/3411106‑3411394
                                                                                                                                                |                                                                                                                                                
gTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTGTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATaaa                                                                                                                                               >  2:1179756/1‑148 (MQ=255)
 ttAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAag                                                                                                                                              >  1:3107116/1‑148 (MQ=255)
  tAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAaga                                                                                                                                             <  2:161485/148‑1 (MQ=255)
  tAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAaga                                                                                                                                             <  1:1444850/148‑1 (MQ=255)
  tAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAaga                                                                                                                                             <  1:1461907/148‑1 (MQ=255)
   aGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAagag                                                                                                                                            <  2:210755/148‑1 (MQ=255)
    gggATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAgg                                                                                                                                           <  2:2768256/148‑1 (MQ=255)
      gATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGt                                                                                                                                         >  1:137649/1‑148 (MQ=255)
      gATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGt                                                                                                                                         >  1:1940314/1‑148 (MQ=255)
          gTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCTAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAg                                                                                                                                     <  2:1032231/148‑1 (MQ=255)
               cGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGtgtt                                                                                                                                >  1:1306511/1‑148 (MQ=255)
                gTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGtgttg                                                                                                                               <  1:2342064/148‑1 (MQ=255)
                 tgctgcTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGtgttgt                                                                                                                              >  1:1612183/1‑148 (MQ=255)
                   ctgctgGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGttgttg                                                                                                                            <  2:735152/148‑1 (MQ=255)
                    tgctgGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGa                                                                                                                           <  2:2843041/148‑1 (MQ=255)
                                cacTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACAATGAt                                                                                                               >  2:2903527/1‑148 (MQ=255)
                                  ctctTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATgg                                                                                                             <  2:3115055/148‑1 (MQ=255)
                                  ctctTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATgg                                                                                                             >  1:576677/1‑148 (MQ=255)
                                   tctTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGc                                                                                                            >  1:2532147/1‑148 (MQ=255)
                                    ctTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCa                                                                                                           >  1:2036505/1‑148 (MQ=255)
                                    ctTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCa                                                                                                           >  1:898733/1‑148 (MQ=255)
                                      ttAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGt                                                                                                         >  2:638214/1‑148 (MQ=255)
                                       tAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTa                                                                                                        >  2:2604909/1‑148 (MQ=255)
                                          tCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACATTTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGTTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGt                                                                                                     <  1:619979/148‑1 (MQ=255)
                                           aaaaaaaaTGAGTTCTAATTTTTGGGGGGCTTGCTAACGACATTTGGCTTGTCGGCCAAAAACGCTTTTTTGATCGTTGATTTCGCTAAAGATCTCATGTATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTa                                                                                                    <  1:470421/147‑1 (MQ=255)
                                             aaaaaaTGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtg                                                                                                  >  2:2183176/1‑148 (MQ=255)
                                               aaaaTGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtgcg                                                                                                >  1:1882730/1‑148 (MQ=255)
                                               aaaaTGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtgcg                                                                                                >  1:3180377/1‑148 (MQ=255)
                                               aaaaTGACGTCTACTTTATGGGGGGCTTGCTACCGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtgcg                                                                                                <  1:3291363/148‑1 (MQ=255)
                                                     acttCTACTTTATGGTGGGGTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAATGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtgcgtctgcg                                                                                          <  1:750933/145‑1 (MQ=255)
                                                      cGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAtgcgtctgcgt                                                                                         >  2:2545268/1‑148 (MQ=255)
                                                          tACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTa                                                                                     >  2:884896/1‑148 (MQ=255)
                                                           aCTTTATGGTGGGCTTGCTATCGACAATTTGCTTGTCGGCCAAAAACTCTATTTTGATCGTTGATTTCGATAAAGATATCATGGATAATGAGGGTATAGTTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTAt                                                                                    <  2:2037149/148‑1 (MQ=255)
                                                           aCTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTAt                                                                                    >  1:2448551/1‑148 (MQ=255)
                                                            cTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATc                                                                                   >  2:2718415/1‑148 (MQ=255)
                                                             tttATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATcc                                                                                  >  1:2923327/1‑148 (MQ=255)
                                                             tttATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATcc                                                                                  >  2:252694/1‑148 (MQ=255)
                                                               tctggtggGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCtg                                                                                <  1:214105/146‑1 (MQ=255)
                                                               tATGGTGGGCTTGCTAACGACAATGGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCtg                                                                                <  2:3445302/148‑1 (MQ=255)
                                                                aTGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCtga                                                                               >  2:1635766/1‑148 (MQ=255)
                                                                 tggtggGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCtgat                                                                              <  1:2775891/148‑1 (MQ=255)
                                                                 tggtggGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCtgat                                                                              >  1:1658104/1‑148 (MQ=255)
                                                                 tggtggGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCtgat                                                                              >  1:247797/1‑148 (MQ=255)
                                                                 tggtggGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCtgat                                                                              <  1:2745611/148‑1 (MQ=255)
                                                                      ggCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTCTCCTGATGACct                                                                         >  1:2660673/1‑148 (MQ=255)
                                                                      ggCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACct                                                                         <  1:2312447/148‑1 (MQ=255)
                                                                      ggCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACct                                                                         >  2:1955461/1‑148 (MQ=255)
                                                                       gCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAATATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACctc                                                                        <  1:1821835/148‑1 (MQ=255)
                                                                       gCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACctc                                                                        <  1:638214/148‑1 (MQ=255)
                                                                        cTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACctct                                                                       <  2:966097/148‑1 (MQ=255)
                                                                        cTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGATATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACctct                                                                       <  1:2276644/148‑1 (MQ=255)
                                                                          tGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACctctct                                                                     >  1:1177071/1‑148 (MQ=255)
                                                                           gCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCtctctc                                                                    >  1:111675/1‑148 (MQ=255)
                                                                           gCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCtctctc                                                                    >  1:2333664/1‑148 (MQ=255)
                                                                              aaCGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGcc                                                                 <  1:1468234/148‑1 (MQ=255)
                                                                              aaCGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGcc                                                                 >  1:3210450/1‑148 (MQ=255)
                                                                               aCGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCt                                                                >  1:2255017/1‑148 (MQ=255)
                                                                                 gACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCttt                                                              >  1:2169926/1‑148 (MQ=255)
                                                                                  aCAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTa                                                             <  2:1827808/148‑1 (MQ=255)
                                                                                  aCAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTa                                                             >  1:362892/1‑148 (MQ=255)
                                                                                     aTTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATtc                                                          >  1:429893/1‑148 (MQ=255)
                                                                                         gCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCgg                                                      >  1:3377646/1‑148 (MQ=255)
                                                                                         gCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCgg                                                      >  1:2969052/1‑148 (MQ=255)
                                                                                           ttGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCg                                                    <  2:1577395/148‑1 (MQ=255)
                                                                                             gTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTa                                                  <  2:426366/148‑1 (MQ=255)
                                                                                              tCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTAt                                                 <  2:3069898/148‑1 (MQ=255)
                                                                                                 gCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTAc                                              >  2:2634669/1‑148 (MQ=255)
                                                                                                   cAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCg                                            >  1:2702131/1‑148 (MQ=255)
                                                                                                   cAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCg                                            >  2:2090806/1‑148 (MQ=255)
                                                                                                    aaaaaCGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCgc                                           >  1:1559797/1‑148 (MQ=255)
                                                                                                       aaCGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCgctag                                        <  2:3023470/148‑1 (MQ=255)
                                                                                                       aaCGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCgctag                                        >  2:2784273/1‑148 (MQ=255)
                                                                                                        aCGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCgctagc                                       <  1:2614988/148‑1 (MQ=255)
                                                                                                         cGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCgctagct                                      >  2:1481612/1‑148 (MQ=255)
                                                                                                           cTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTAt                                    >  2:3262212/1‑148 (MQ=255)
                                                                                                            tATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATc                                   >  1:554087/1‑148 (MQ=255)
                                                                                                            tATTTTGATCGTTGAGTTCACTAAAGATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATc                                   >  2:174865/1‑148 (MQ=255)
                                                                                                             aTTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCa                                  >  2:1401467/1‑148 (MQ=255)
                                                                                                              ttttGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAg                                 >  2:1955926/1‑148 (MQ=255)
                                                                                                               tttGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGt                                >  2:669934/1‑148 (MQ=255)
                                                                                                                ttGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTa                               <  1:1800450/148‑1 (MQ=255)
                                                                                                                 tGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTaa                              <  1:100622/148‑1 (MQ=255)
                                                                                                                    tCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACgg                           >  1:2591051/1‑148 (MQ=255)
                                                                                                                    tCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGGTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTCACgg                           >  2:486208/1‑148 (MQ=255)
                                                                                                                      gTTGAGTTCGCTAAATATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTg                         <  2:1821482/148‑1 (MQ=255)
                                                                                                                      gTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTg                         <  1:2183176/148‑1 (MQ=255)
                                                                                                                      gTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTg                         <  2:1940314/148‑1 (MQ=255)
                                                                                                                      gTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTg                         <  2:137649/148‑1 (MQ=255)
                                                                                                                         gAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCg                      >  1:1523632/1‑148 (MQ=255)
                                                                                                                                cTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTCCCGCTAGCTATCAGTAACGGTGCCGGCAGTgg               >  2:787781/1‑148 (MQ=255)
                                                                                                                                cTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTgg               >  2:3406071/1‑148 (MQ=255)
                                                                                                                                cTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTgg               >  1:2808557/1‑148 (MQ=255)
                                                                                                                                 tAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGgc              >  2:1449680/1‑148 (MQ=255)
                                                                                                                                 tAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGgc              >  2:676115/1‑148 (MQ=255)
                                                                                                                                 tAAAGATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGgc              >  2:3277588/1‑148 (MQ=255)
                                                                                                                                  aaaGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGgcg             >  1:705786/1‑148 (MQ=255)
                                                                                                                                         tcATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGaa      >  2:3427416/1‑148 (MQ=255)
                                                                                                                                         tcATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGaa      >  2:1350006/1‑148 (MQ=255)
                                                                                                                                           aTGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACg    <  2:2165774/148‑1 (MQ=255)
                                                                                                                                             ggATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCt  >  2:2265653/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCT  >  CP009273/3411106‑3411394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGCCGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGG  >  CP009273/3411101‑3411399
                                                                                                                                                     |                                                                                                                                                     
GTGCCGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGAT                                                                                                                                                       >  SRR19973066.1218930/1‑150 (MQ=60)
GTGCCGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATCACGTCTACTTTATTGTTGGCATGCTAAAGACAATTGGCTTGTCGGCCAAAAAAGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGAT                                                                                                                                                       >  SRR19973066.427238/1‑150 (MQ=60)
    CGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAG                                                                                                                                                   >  SRR19973066.3127864/1‑150 (MQ=60)
     GTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGA                                                                                                                                                  >  SRR19973066.672194/1‑150 (MQ=60)
       TAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGG                                                                                                                                                <  SRR19973066.1455126/150‑1 (MQ=60)
       TAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGG                                                                                                                                                <  SRR19973066.1455134/150‑1 (MQ=60)
       TAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGG                                                                                                                                                <  SRR19973066.1472245/150‑1 (MQ=60)
         GGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTCAAGATCTCATGGATAAAGAGGGT                                                                                                                                              >  SRR19973066.1954075/1‑150 (MQ=60)
         GGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGT                                                                                                                                              >  SRR19973066.1552497/1‑150 (MQ=60)
         GGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGT                                                                                                                                              >  SRR19973066.138979/1‑150 (MQ=60)
               GTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGT                                                                                                                                        <  SRR19973066.48024/150‑1 (MQ=60)
                 CGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGT                                                                                                                                      <  SRR19973066.1937146/150‑1 (MQ=60)
                  GGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTT                                                                                                                                     >  SRR19973066.1315985/1‑150 (MQ=60)
                  GGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTT                                                                                                                                     <  SRR19973066.1489936/150‑1 (MQ=60)
                    CGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGT                                                                                                                                   >  SRR19973066.3405850/1‑150 (MQ=60)
                    CGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGT                                                                                                                                   >  SRR19973066.1623877/1‑150 (MQ=60)
                     GTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTT                                                                                                                                  <  SRR19973066.18983/150‑1 (MQ=60)
                     GTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTT                                                                                                                                  <  SRR19973066.2358010/150‑1 (MQ=60)
                     GTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTT                                                                                                                                  <  SRR19973066.2358004/150‑1 (MQ=60)
                                  CGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGA                                                                                                                     >  SRR19973066.740856/1‑150 (MQ=60)
                                   GACACTCTTTAATAAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACTATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGAT                                                                                                                    <  SRR19973066.1363990/150‑1 (MQ=60)
                                    ACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATG                                                                                                                   <  SRR19973066.1477327/150‑1 (MQ=60)
                                    ACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATG                                                                                                                   >  SRR19973066.2412635/1‑150 (MQ=60)
                                     CACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGG                                                                                                                  >  SRR19973066.580735/1‑150 (MQ=60)
                                      ACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGC                                                                                                                 >  SRR19973066.2549360/1‑150 (MQ=60)
                                      ACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGC                                                                                                                 >  SRR19973066.1169291/1‑150 (MQ=60)
                                       CTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCA                                                                                                                >  SRR19973066.2559338/1‑150 (MQ=60)
                                       CTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCA                                                                                                                >  SRR19973066.2050709/1‑150 (MQ=60)
                                       CTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCA                                                                                                                >  SRR19973066.277568/1‑150 (MQ=60)
                                       CTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCA                                                                                                                >  SRR19973066.905078/1‑150 (MQ=60)
                                              ATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTA                                                                                                         >  SRR19973066.3034599/1‑150 (MQ=60)
                                               TCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACATTTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGTTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAT                                                                                                        <  SRR19973066.624373/150‑1 (MQ=60)
                                               TCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTAT                                                                                                        <  SRR19973066.2262678/150‑1 (MQ=60)
                                                CAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATG                                                                                                       >  SRR19973066.233419/1‑150 (MQ=60)
                                                AAAAAAAATGAGTTCTAATTTTTGGGGGGCTTGCTAACGACATTTGGCTTGTCGGCCAAAAACGCTTTTTTGATCGTTGATTTCGCTAAAGATCTCATGTATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATG                                                                                                       <  SRR19973066.473991/150‑1 (MQ=60)
                                                  AAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCG                                                                                                     >  SRR19973066.1896166/1‑150 (MQ=60)
                                                  AAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCG                                                                                                     >  SRR19973066.3201536/1‑150 (MQ=60)
                                                  AAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCG                                                                                                     >  SRR19973066.633397/1‑150 (MQ=60)
                                                    AAAATGACGTCTACTTTATGGGGGGCTTGCTACCGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTC                                                                                                   <  SRR19973066.3313077/150‑1 (MQ=60)
                                                     AAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCT                                                                                                  <  SRR19973066.2310709/150‑1 (MQ=60)
                                                          ACTTCTACTTTATGGTGGGGTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAATGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTC                                                                                             <  SRR19973066.756506/150‑1 (MQ=60)
                                                           CGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCC                                                                                            >  SRR19973066.2274351/1‑150 (MQ=60)
                                                            GTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCT                                                                                           >  SRR19973066.1635409/1‑150 (MQ=60)
                                                            GTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCAAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGACGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCT                                                                                           <  SRR19973066.624045/150‑1 (MQ=60)
                                                              CTACTTTATGGTGGGCTTGCTAACTACAATTGGCTTGTCGGACAAAAACGCTATTTTGATCTTTGAGTTCGCTAAAGATCTCATGGATAAAGAGTGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCTTCCTAT                                                                                         >  SRR19973066.2466661/1‑150 (MQ=60)
                                                              CTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTAT                                                                                         >  SRR19973066.782521/1‑150 (MQ=60)
                                                              CTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTAT                                                                                         >  SRR19973066.2465235/1‑150 (MQ=60)
                                                                ACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCC                                                                                       >  SRR19973066.2942807/1‑150 (MQ=60)
                                                                 CTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCT                                                                                      <  SRR19973066.2959622/150‑1 (MQ=60)
                                                                    TCTGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGAT                                                                                   <  SRR19973066.215871/150‑1 (MQ=60)
                                                                    TATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGAT                                                                                   >  SRR19973066.249711/1‑150 (MQ=60)
                                                                    TATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGAT                                                                                   >  SRR19973066.1670183/1‑150 (MQ=60)
                                                                     ATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATG                                                                                  <  SRR19973066.1997885/150‑1 (MQ=60)
                                                                      TGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGA                                                                                 <  SRR19973066.2794577/150‑1 (MQ=60)
                                                                      TGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGA                                                                                 <  SRR19973066.2764082/150‑1 (MQ=60)
                                                                         TGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTCTCCTGATGACCT                                                                              >  SRR19973066.2678505/1‑150 (MQ=60)
                                                                         TGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCT                                                                              >  SRR19973066.2678657/1‑150 (MQ=60)
                                                                           GGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCT                                                                            >  SRR19973066.3136612/1‑150 (MQ=60)
                                                                           GGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCT                                                                            <  SRR19973066.2328266/150‑1 (MQ=60)
                                                                            GCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAATATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTC                                                                           <  SRR19973066.1834823/150‑1 (MQ=60)
                                                                            GCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTC                                                                           <  SRR19973066.642817/150‑1 (MQ=60)
                                                                             CTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCT                                                                          >  SRR19973066.1185362/1‑150 (MQ=60)
                                                                             CTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGATATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCT                                                                          <  SRR19973066.2292285/150‑1 (MQ=60)
                                                                              TTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTC                                                                         >  SRR19973066.2349581/1‑150 (MQ=60)
                                                                              TTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTC                                                                         >  SRR19973066.112816/1‑150 (MQ=60)
                                                                                 CTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCC                                                                      >  SRR19973066.3231731/1‑150 (MQ=60)
                                                                                  TAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCT                                                                     >  SRR19973066.2270561/1‑150 (MQ=60)
                                                                                  TAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCT                                                                     >  SRR19973066.2611039/1‑150 (MQ=60)
                                                                                   AACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTT                                                                    <  SRR19973066.1478592/150‑1 (MQ=60)
                                                                                    ACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTT                                                                   >  SRR19973066.2185007/1‑150 (MQ=60)
                                                                                    ACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTT                                                                   >  SRR19973066.2184993/1‑150 (MQ=60)
                                                                                     CGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTA                                                                  >  SRR19973066.365738/1‑150 (MQ=60)
                                                                                       ACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATT                                                                >  SRR19973066.1614702/1‑150 (MQ=60)
                                                                                       ACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATT                                                                >  SRR19973066.3394795/1‑150 (MQ=60)
                                                                                        CAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTC                                                               >  SRR19973066.433227/1‑150 (MQ=60)
                                                                                        CAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTC                                                               >  SRR19973066.433217/1‑150 (MQ=60)
                                                                                          ATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTACAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTC                                                             >  SRR19973066.1834467/1‑150 (MQ=60)
                                                                                          ATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTC                                                             <  SRR19973066.2165851/150‑1 (MQ=60)
                                                                                            TGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGG                                                           >  SRR19973066.2988682/1‑150 (MQ=60)
                                                                                            TGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGG                                                           >  SRR19973066.3400171/1‑150 (MQ=60)
                                                                                               CTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGT                                                        >  SRR19973066.560191/1‑150 (MQ=60)
                                                                                               CTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGT                                                        >  SRR19973066.3226587/1‑150 (MQ=60)
                                                                                                      GCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCG                                                 >  SRR19973066.2720228/1‑150 (MQ=60)
                                                                                                       CCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGC                                                >  SRR19973066.1571021/1‑150 (MQ=60)
                                                                                                         AAAAACGCTATTTTGATCGTTTAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTA                                              >  SRR19973066.2733667/1‑150 (MQ=60)
                                                                                                         AAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTA                                              >  SRR19973066.404292/1‑150 (MQ=60)
                                                                                                             ACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTA                                          <  SRR19973066.2632659/150‑1 (MQ=60)
                                                                                                               GCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATC                                        >  SRR19973066.558063/1‑150 (MQ=60)
                                                                                                                CTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCA                                       >  SRR19973066.2836358/1‑150 (MQ=60)
                                                                                                                     TTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAAC                                  <  SRR19973066.254623/150‑1 (MQ=60)
                                                                                                                     TTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAAC                                  <  SRR19973066.1813244/150‑1 (MQ=60)
                                                                                                                     TTGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAAC                                  <  SRR19973066.2068277/150‑1 (MQ=60)
                                                                                                                      TGATCGTTTTTTTCGCTAAAGATCTCATGGATAAAGTGTGTAAAGGTTTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACG                                 <  SRR19973066.3304396/150‑1 (MQ=60)
                                                                                                                      TGATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACG                                 <  SRR19973066.101692/150‑1 (MQ=60)
                                                                                                                       GATCGTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGG                                >  SRR19973066.2608608/1‑150 (MQ=60)
                                                                                                                           GTTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCC                            <  SRR19973066.2198328/150‑1 (MQ=60)
                                                                                                                            TTGAGTTCGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCG                           >  SRR19973066.1534400/1‑150 (MQ=60)
                                                                                                                                   CGCTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGG                    >  SRR19973066.2827452/1‑150 (MQ=60)
                                                                                                                                     CTAAAGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCG                  >  SRR19973066.711004/1‑150 (MQ=60)
                                                                                                                                         AGATCTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCA              >  SRR19973066.1405374/1‑150 (MQ=60)
                                                                                                                                             CTCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAAC          >  SRR19973066.265201/1‑150 (MQ=60)
                                                                                                                                              TCATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACG         >  SRR19973066.1731056/1‑150 (MQ=60)
                                                                                                                                               CATGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGC        >  SRR19973066.1189182/1‑150 (MQ=60)
                                                                                                                                                 TGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTCTCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTG      >  SRR19973066.1305443/1‑150 (MQ=60)
                                                                                                                                                 TGGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTG      >  SRR19973066.1304905/1‑150 (MQ=60)
                                                                                                                                                  GGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGT     >  SRR19973066.2853532/1‑150 (MQ=60)
                                                                                                                                                  GGATAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGT     >  SRR19973066.76938/1‑150 (MQ=60)
                                                                                                                                                     TAAAGTGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGG  <  SRR19973066.335082/150‑1 (MQ=60)
                                                                                                                                                     TAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGG  >  SRR19973066.1206754/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GTGCCGTTAGGGATTGTCGGCGTGCTGCTGGCGGCGACACTCTTTAATCAAAAAAATGACGTCTACTTTATGGTGGGCTTGCTAACGACAATTGGCTTGTCGGCCAAAAACGCTATTTTGATCGTTGAGTTCGCTAAAGATCTCATGGAGAAAGAGGGTAAAGGTGTTGTTGAAGCGACACTGATGGCAGTACGTATGCGTCTGCGTCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGG  >  CP009273/3411101‑3411399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑