Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,422,726 G→T 94.9% V44V (GTG→GTT)  yrdA → bacterial transferase hexapeptide domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,422,7260GT94.9% 581.4 / 21.1 198V44V (GTG→GTT) yrdAbacterial transferase hexapeptide domain protein
Reads supporting (aligned to +/- strand):  ref base G (5/5);  new base T (100/88);  total (105/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 201 total aligned reads displayed.

TTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAG  >  CP009273/3422581‑3422870
                                                                                                                                                 |                                                                                                                                                
ttGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTAt                                                                                                                                                >  1:1971404/1‑148 (MQ=255)
 tGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTTTTAtt                                                                                                                                               >  1:2279751/1‑148 (MQ=255)
   cTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCg                                                                                                                                             >  1:445985/1‑148 (MQ=255)
   cTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCg                                                                                                                                             <  2:1058226/148‑1 (MQ=255)
    tGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGt                                                                                                                                            <  1:367044/148‑1 (MQ=255)
     gAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTg                                                                                                                                           >  1:2923703/1‑148 (MQ=255)
      aGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTgg                                                                                                                                          >  1:2087666/1‑148 (MQ=255)
      aGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTgg                                                                                                                                          >  1:293479/1‑148 (MQ=255)
       ggTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGga                                                                                                                                         >  2:1688843/1‑148 (MQ=255)
        gTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGgag                                                                                                                                        <  1:1553381/148‑1 (MQ=255)
            gTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGt                                                                                                                                    >  2:2777159/1‑148 (MQ=255)
            gTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGt                                                                                                                                    >  2:1000875/1‑148 (MQ=255)
               tGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACa                                                                                                                                 <  1:2981020/148‑1 (MQ=255)
               tGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACa                                                                                                                                 <  1:1248481/148‑1 (MQ=255)
                 tCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACAtt                                                                                                                               >  1:1051466/1‑148 (MQ=255)
                 tCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACAtt                                                                                                                               >  2:613905/1‑148 (MQ=255)
                 tCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACAtt                                                                                                                               >  2:552622/1‑148 (MQ=255)
                  cTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTTGAGATGTTCATTa                                                                                                                              >  1:3138636/1‑148 (MQ=255)
                    gATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATg                                                                                                                            <  1:1350692/148‑1 (MQ=255)
                    gATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATg                                                                                                                            <  2:1600672/148‑1 (MQ=255)
                     aTGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGt                                                                                                                           <  2:2685089/148‑1 (MQ=255)
                         tttACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAg                                                                                                                       >  1:1390980/1‑148 (MQ=255)
                         tttACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAg                                                                                                                       <  1:2718478/148‑1 (MQ=255)
                          ttACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATTATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGa                                                                                                                      <  2:835609/148‑1 (MQ=255)
                            aCGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATc                                                                                                                    >  2:1348726/1‑148 (MQ=255)
                             cGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCg                                                                                                                   <  2:625634/148‑1 (MQ=255)
                               cccATACCGCGATCTTTTTCCACAAATCGGTCCGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGa                                                                                                                 >  2:2601124/1‑148 (MQ=255)
                               cccATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGa                                                                                                                 >  2:819416/1‑148 (MQ=255)
                               cccATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGa                                                                                                                 <  1:992910/148‑1 (MQ=255)
                                ccATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAg                                                                                                                <  1:2196733/148‑1 (MQ=255)
                                 cATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGTTCGGAgc                                                                                                               >  1:552620/1‑148 (MQ=255)
                                  aTACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAgca                                                                                                              <  1:2103497/148‑1 (MQ=255)
                                   tACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAgcac                                                                                                             <  1:1253643/148‑1 (MQ=255)
                                   tACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAgcac                                                                                                             >  2:2837202/1‑148 (MQ=255)
                                     ccGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAgcacgc                                                                                                           >  2:1522190/1‑148 (MQ=255)
                                     ccGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAgcacgc                                                                                                           >  1:469990/1‑148 (MQ=255)
                                        cgATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCAcc                                                                                                        >  2:2463267/1‑148 (MQ=255)
                                        cgATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGTGATGTACATTATGTACAGATCGGAGCACGCAcc                                                                                                        >  1:1096848/1‑148 (MQ=255)
                                        cgATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATGTACATTATGTACAGATCGGAGCACGCAcc                                                                                                        >  2:680369/1‑148 (MQ=255)
                                         gATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCa                                                                                                       >  2:1855113/1‑148 (MQ=255)
                                           tCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAat                                                                                                     >  2:2142331/1‑148 (MQ=255)
                                            cTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATGTACATTATTTACAGATAGGAGCACGCACCaaaa                                                                                                    >  1:2396229/1‑146 (MQ=255)
                                             tttttCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAatat                                                                                                   <  1:1760800/148‑1 (MQ=255)
                                               tttCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATcc                                                                                                 <  1:1277085/148‑1 (MQ=255)
                                                 tCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAg                                                                                               <  2:3134064/148‑1 (MQ=255)
                                                 tCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAg                                                                                               <  2:1365838/148‑1 (MQ=255)
                                                       aaTCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGc                                                                                         >  2:1450127/1‑148 (MQ=255)
                                                          cGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGt                                                                                      <  2:1971404/148‑1 (MQ=255)
                                                          cGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGt                                                                                      >  1:2949109/1‑148 (MQ=255)
                                                          cGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGt                                                                                      <  1:192614/148‑1 (MQ=255)
                                                           ggTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTa                                                                                     <  1:2462168/148‑1 (MQ=255)
                                                           ggTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTa                                                                                     <  2:1285505/148‑1 (MQ=255)
                                                           ggTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTa                                                                                     <  1:284592/148‑1 (MQ=255)
                                                               aGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGtt                                                                                 >  1:2382036/1‑148 (MQ=255)
                                                                 cgcgTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGc                                                                               >  1:1466935/1‑148 (MQ=255)
                                                                   cgTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCAt                                                                             <  2:2376108/148‑1 (MQ=255)
                                                                      aaTGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATAGGAGCACGCACCAAAATCCAGGATGGCAGTATTTTTCATGTc                                                                          >  1:3427143/1‑148 (MQ=255)
                                                                        tGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCAc                                                                        <  1:441312/148‑1 (MQ=255)
                                                                         gATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACt                                                                       <  2:2994443/148‑1 (MQ=255)
                                                                          aTCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTc                                                                      <  2:379969/148‑1 (MQ=255)
                                                                          aTCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTc                                                                      <  2:2637493/148‑1 (MQ=255)
                                                                           tCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGGCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCCGTATGTTGCATGTCACTCa                                                                     >  2:797858/1‑148 (MQ=255)
                                                                            cgacgaTAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCAt                                                                    >  2:1426063/1‑148 (MQ=255)
                                                                              acgaTAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATaa                                                                  <  2:2650610/148‑1 (MQ=255)
                                                                              acgaTAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATaa                                                                  <  2:3151407/148‑1 (MQ=255)
                                                                                  tAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATcc                                                              <  2:3325077/148‑1 (MQ=255)
                                                                                  tAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATcc                                                              <  1:1576468/148‑1 (MQ=255)
                                                                                    gCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATTTAAATTATTTACAGATAGGAGCACGCACCAATATACAGGATGGCAGTATTTTGCATGTCACTCAAAAATACTc                                                            >  1:3406847/1‑148 (MQ=255)
                                                                                     cAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGCGATTTACATTATTTACAGATCGGAGCACGCCCCAATATCCAGGATGGGAGTATGTTGCATGTCAATCAAAAAAccacc                                                           >  1:770434/1‑145 (MQ=255)
                                                                                        tgtCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTAc                                                        <  2:2042339/148‑1 (MQ=255)
                                                                                           cGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTAAAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACACATAAATCCACGTACAAc                                                     >  1:3480873/1‑148 (MQ=255)
                                                                                            gTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAAcc                                                    <  1:1000875/148‑1 (MQ=255)
                                                                                             tGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCCACTTGTTATTCGTCGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATACTCGTACAAccc                                                   >  1:3489990/1‑148 (MQ=255)
                                                                                                 tGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGAt                                               >  1:719971/1‑148 (MQ=255)
                                                                                                  ggTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATg                                              <  1:2363318/148‑1 (MQ=255)
                                                                                                   gTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGGGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATgg                                             >  2:754355/1‑148 (MQ=255)
                                                                                                    tGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGGTGTTATTAGTGGAGATGTACATTATTTAAAGATCGGAGCACGCACCAATATACAGGATGGGAGTATGTTGCATGTCACTCCTAAATACTCGTACAACCCACATGGc                                            >  1:2139857/1‑148 (MQ=255)
                                                                                                       cGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAAc                                         >  2:3209832/1‑148 (MQ=255)
                                                                                                          tCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATTTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCa                                      >  1:823093/1‑148 (MQ=255)
                                                                                                          tCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGGACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCa                                      <  2:1994346/148‑1 (MQ=255)
                                                                                                          tCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGGTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGCTGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCa                                      >  1:1903298/1‑148 (MQ=255)
                                                                                                             tCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATTTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTa                                   >  1:1175543/1‑148 (MQ=255)
                                                                                                               tGGCTGATGATGTGGGGATCTGGCCGCTCGTTTTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAAAATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATACTCGTACAACCCAGATGGCAACCCATAAAc                                 >  1:1281894/1‑148 (MQ=255)
                                                                                                               tGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAAc                                 <  1:528413/148‑1 (MQ=255)
                                                                                                               tGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAAc                                 <  1:2132566/148‑1 (MQ=255)
                                                                                                                ggCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATGTACAATATGTACACATCGGAGCACGCCCCAAAATCCAGGATGGCCGTATGTTGCATGTCACTCATAAATACTCGTACAACCCACATGGCAACCCAGTAAcc                                >  1:139809/1‑148 (MQ=255)
                                                                                                                  cTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCAt                              <  2:1886174/148‑1 (MQ=255)
                                                                                                                  cTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCAt                              <  2:2329695/148‑1 (MQ=255)
                                                                                                                   tgatgaTGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCAtt                             >  2:86472/1‑148 (MQ=255)
                                                                                                                    gatgatGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTg                            <  1:1526734/148‑1 (MQ=255)
                                                                                                                        atgTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGa                        <  1:1688513/148‑1 (MQ=255)
                                                                                                                          gtgGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAg                      >  2:3257714/1‑148 (MQ=255)
                                                                                                                          gtgGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAg                      <  2:2862368/148‑1 (MQ=255)
                                                                                                                             gggATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCAAGTCACACATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATg                   >  2:512974/1‑148 (MQ=255)
                                                                                                                              ggATCTGGCCGCTCGTTGTTATTCTTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAAAAACCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCACGTAAAACCCAGATTGCAACCCATTATCCATTGGCGAATATGt                  >  2:2975112/1‑148 (MQ=255)
                                                                                                                                aTCTGGCCGCTAGTTGTTATTCGTGGAGATGTACATTATGTACAGATAGGAGCACGCACCAATATCCAGGATGTCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCa                <  2:2279751/148‑1 (MQ=255)
                                                                                                                                 tCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCAc               >  2:1138734/1‑148 (MQ=255)
                                                                                                                                        gCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGt        <  2:2842860/148‑1 (MQ=255)
                                                                                                                                              tgttATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAg  >  1:3241875/1‑148 (MQ=255)
                                                                                                                                              tgttATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAg  >  1:574056/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAG  >  CP009273/3422581‑3422870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTATTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTG  >  CP009273/3422578‑3422873
                                                                                                                                                    |                                                                                                                                                   
TTATTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTA                                                                                                                                                    <  SRR19973067.984990/150‑1 (MQ=60)
 TATTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTAT                                                                                                                                                   >  SRR19973067.1984770/1‑150 (MQ=60)
  ATTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTTTTATT                                                                                                                                                  >  SRR19973067.2294830/1‑150 (MQ=60)
    TGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCG                                                                                                                                                >  SRR19973067.449079/1‑150 (MQ=60)
    TGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCG                                                                                                                                                >  SRR19973067.448945/1‑150 (MQ=60)
      CTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTG                                                                                                                                              >  SRR19973067.2942283/1‑150 (MQ=60)
       TGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGG                                                                                                                                             >  SRR19973067.2101483/1‑150 (MQ=60)
       TGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGG                                                                                                                                             <  SRR19973067.369595/150‑1 (MQ=60)
       TGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGG                                                                                                                                             >  SRR19973067.295363/1‑150 (MQ=60)
           GTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGAT                                                                                                                                         <  SRR19973067.1564013/150‑1 (MQ=60)
           GTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGAT                                                                                                                                         <  SRR19973067.1563964/150‑1 (MQ=60)
           GTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGACGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGAT                                                                                                                                         <  SRR19973067.3336823/150‑1 (MQ=60)
            TAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATG                                                                                                                                        <  SRR19973067.475160/150‑1 (MQ=60)
                  TGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATT                                                                                                                                  <  SRR19973067.1256909/150‑1 (MQ=60)
                  TGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATT                                                                                                                                  <  SRR19973067.2999851/150‑1 (MQ=60)
                  TGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATT                                                                                                                                  <  SRR19973067.1258862/150‑1 (MQ=60)
                  TGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATT                                                                                                                                  >  SRR19973067.1058679/1‑150 (MQ=60)
                   GTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTA                                                                                                                                 <  SRR19973067.1383461/150‑1 (MQ=60)
                   GTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTTGAGATGTTCATTA                                                                                                                                 >  SRR19973067.3158566/1‑150 (MQ=60)
                       GATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTA                                                                                                                             <  SRR19973067.263710/150‑1 (MQ=60)
                       GATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTA                                                                                                                             <  SRR19973067.1359923/150‑1 (MQ=60)
                          GTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAG                                                                                                                          >  SRR19973067.1400433/1‑150 (MQ=60)
                          GTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGGTGTTATTAGTGGAGATGTACATTATTTACAG                                                                                                                          >  SRR19973067.1400487/1‑150 (MQ=60)
                            TTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGAT                                                                                                                        <  SRR19973067.2736004/150‑1 (MQ=60)
                                 GCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAG                                                                                                                   <  SRR19973067.1651487/150‑1 (MQ=60)
                                  CCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGTTCGGAGC                                                                                                                  >  SRR19973067.556254/1‑150 (MQ=60)
                                  CCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATAGGCGC                                                                                                                  >  SRR19973067.741688/1‑150 (MQ=60)
                                  CCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGC                                                                                                                  <  SRR19973067.999745/150‑1 (MQ=60)
                                   CCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCA                                                                                                                 <  SRR19973067.2211389/150‑1 (MQ=60)
                                     ATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACG                                                                                                               <  SRR19973067.2117454/150‑1 (MQ=60)
                                      TACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGC                                                                                                              <  SRR19973067.1262122/150‑1 (MQ=60)
                                      TACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGC                                                                                                              >  SRR19973067.473199/1‑150 (MQ=60)
                                      TACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGC                                                                                                              >  SRR19973067.473244/1‑150 (MQ=60)
                                         CGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATTTACATTATGTACAGATCGGAGCACGCCCC                                                                                                           >  SRR19973067.1930069/1‑150 (MQ=60)
                                         CGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGTGATGTACATTATGTACAGATCGGAGCACGCACC                                                                                                           >  SRR19973067.1104320/1‑150 (MQ=60)
                                           CGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGTGATGTAAATTATGTACAGATCGGAGCACGCACCCA                                                                                                         >  SRR19973067.324483/1‑150 (MQ=60)
                                             ATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGTGATGTAAATTATTTACAGATCGGAGCACGCCCCAAAA                                                                                                       >  SRR19973067.2411793/1‑150 (MQ=60)
                                             ATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATGTACATTATTTACAGATAGGAGCACGCACCAAAA                                                                                                       >  SRR19973067.2411837/1‑150 (MQ=60)
                                              TCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGGTGTTATTAGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATAT                                                                                                      >  SRR19973067.3467552/1‑150 (MQ=60)
                                                TTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCC                                                                                                    <  SRR19973067.1772785/150‑1 (MQ=60)
                                                  TTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAG                                                                                                  <  SRR19973067.1285780/150‑1 (MQ=60)
                                                  TTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGATGATGATGTGGGGATGTTGCCGCTCGTTGTTATTCGTGGAGATGTAAATTATGTACAGATCGGAGAACGCACCAATATCCAG                                                                                                  <  SRR19973067.1285515/150‑1 (MQ=60)
                                                      CACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATG                                                                                              <  SRR19973067.1964033/150‑1 (MQ=60)
                                                        CAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGC                                                                                            <  SRR19973067.236167/150‑1 (MQ=60)
                                                         AAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATACAGGATGGCA                                                                                           >  SRR19973067.3117112/1‑150 (MQ=60)
                                                         AAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATGTACAATATGTACAGATAGGAGCACGCACCAATATACAGGATGGCA                                                                                           >  SRR19973067.1416137/1‑150 (MQ=60)
                                                           ATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAAAATCCAGGATGGCAGT                                                                                         >  SRR19973067.2967772/1‑150 (MQ=60)
                                                             CGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTAT                                                                                       <  SRR19973067.194105/150‑1 (MQ=60)
                                                              GGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATG                                                                                      <  SRR19973067.2478353/150‑1 (MQ=60)
                                                              GGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATG                                                                                      <  SRR19973067.286451/150‑1 (MQ=60)
                                                                TCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTT                                                                                    >  SRR19973067.2397504/1‑150 (MQ=60)
                                                                 CAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTG                                                                                   >  SRR19973067.2975002/1‑150 (MQ=60)
                                                                  AGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGC                                                                                  >  SRR19973067.1476719/1‑150 (MQ=60)
                                                                  AGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATTTACAATATTTTCAGAACGGAGCACGCACCAAAAAACAGGATGGCAGTATGTTGC                                                                                  >  SRR19973067.360714/1‑150 (MQ=60)
                                                                      CGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGT                                                                              >  SRR19973067.2393178/1‑150 (MQ=60)
                                                                       GTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATAGGAGCACGCACCAAAATCCAGGATGGCAGTATTTTTCATGTC                                                                             >  SRR19973067.3448953/1‑150 (MQ=60)
                                                                           TGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATTTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTC                                                                         >  SRR19973067.900498/1‑150 (MQ=60)
                                                                           TGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTC                                                                         <  SRR19973067.444378/150‑1 (MQ=60)
                                                                            GATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCA                                                                        <  SRR19973067.656498/150‑1 (MQ=60)
                                                                               CGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAA                                                                     <  SRR19973067.1524790/150‑1 (MQ=60)
                                                                               CGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAA                                                                     <  SRR19973067.1700473/150‑1 (MQ=60)
                                                                                     TAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTC                                                               <  SRR19973067.1587263/150‑1 (MQ=60)
                                                                                     TAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATTTAAATTATTTACAGATAGGAGCACGCACCAATATACAGGATGGCAGTATTTTGCATGTCACTCAAAAATACTC                                                               >  SRR19973067.3428534/1‑150 (MQ=60)
                                                                                     TAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGGTGTTATTCGTGGAGATTTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTC                                                               >  SRR19973067.3428551/1‑150 (MQ=60)
                                                                                      AGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATTTACAGATCGGAGCACGCACCAATATACAGGATGGCAGTATGTTGCATGTCACTCAAAAATACTCG                                                              >  SRR19973067.545716/1‑150 (MQ=60)
                                                                                      AGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGCGATTTACATTATTTACAGATCGGAGCACGCCCCAATATCCAGGATGGGAGTATGTTGCATGTCAatcaaaaaaccacc                                                              >  SRR19973067.775774/1‑136 (MQ=60)
                                                                                        CAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGAAGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGATATGTAACATATGTACACATAGGACCACGCACCAAAAACCAGGAAGGCAGTATGTTGCATGTaacacataaaaaatcgga                                                            >  SRR19973067.3428324/1‑132 (MQ=60)
                                                                                            GTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTAAAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACACATAAATCCACGTACAAC                                                        >  SRR19973067.3502961/1‑150 (MQ=60)
                                                                                            GTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGGTGTTATTAGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCCAAAAACAGGATTGCAGTATGTTTCATGTCACTCAaaaaaactcgttaaac                                                        >  SRR19973067.2119139/1‑134 (MQ=60)
                                                                                              CGTGATTGGTGACGTTCGTCTGGCTGATGATTTGGGGATCTGGCCGAAACATGTTATTAGTCCACTAGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCC                                                      >  SRR19973067.3512008/1‑150 (MQ=60)
                                                                                              CGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCC                                                      >  SRR19973067.2958851/1‑150 (MQ=60)
                                                                                              CGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCCACTTGTTATTCGTCGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATACTCGTACAACCC                                                      >  SRR19973067.3512144/1‑150 (MQ=60)
                                                                                               GTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCA                                                     <  SRR19973067.1007757/150‑1 (MQ=60)
                                                                                               GTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCA                                                     <  SRR19973067.2795099/150‑1 (MQ=60)
                                                                                                  ATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGAT                                                  >  SRR19973067.724970/1‑150 (MQ=60)
                                                                                                     GGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGC                                               <  SRR19973067.2378717/150‑1 (MQ=60)
                                                                                                     GGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGGTGTTATTAGTGGAGATGTACATTATTTAAAGATCGGAGCACGCACCAATATACAGGATGGGAGTATGTTGCATGTCACTCCTAAATACTCGTACAACCCACATGGC                                               >  SRR19973067.2154162/1‑150 (MQ=60)
                                                                                                     GGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGGTGTTATTAGTGGAGATGTAAAATATGTACAGATCCGAGCACCCACCACTAACCACGAAGGCAGTATTTTTGATGTCACTCAAAAATACTCGTACAACACACATGGC                                               >  SRR19973067.2154159/1‑150 (MQ=55)
                                                                                                       TGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAA                                             >  SRR19973067.2903624/1‑150 (MQ=60)
                                                                                                           GTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTTTTATTAGTGGAGATTTACAATATGTACAGATCGGAGCACGCACCAAAATACAGGATGGCAGTATGTTGCATGTCACTCATAAAACCTCGTACAACCCAGATGGCAACCCA                                         >  SRR19973067.106672/1‑150 (MQ=60)
                                                                                                           GTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATTTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCA                                         >  SRR19973067.828722/1‑150 (MQ=60)
                                                                                                           GTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGGTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGCTGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCA                                         >  SRR19973067.1916273/1‑150 (MQ=60)
                                                                                                              CGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATTTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTA                                      >  SRR19973067.1183322/1‑150 (MQ=60)
                                                                                                              CGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAAAATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTA                                      >  SRR19973067.1183396/1‑150 (MQ=60)
                                                                                                                TCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTTTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAAAATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATACTCGTACAACCCAGATGGCAACCCATAAAC                                    >  SRR19973067.1290626/1‑150 (MQ=60)
                                                                                                                TCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAAC                                    <  SRR19973067.2263106/150‑1 (MQ=60)
                                                                                                                TCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATGTACATTATTTAAAGATCGGAGCACGCACCAAAAAACAGGATGGCAGTATGTTGCATGTCACTCATAAAAACTCGTACAACCCACATTGCAACCCAATAAC                                    >  SRR19973067.1290624/1‑150 (MQ=60)
                                                                                                                 CTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATGTACAATATGTACACATCGGAGCACGCCCCAAAATCCAGGATGGCCGTATGTTGCATGTCACTCATAAATACTCGTACAACCCACATGGCAACCCAGTAACC                                   >  SRR19973067.141049/1‑150 (MQ=60)
                                                                                                                  TGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCA                                  <  SRR19973067.2146806/150‑1 (MQ=60)
                                                                                                                  TGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCA                                  <  SRR19973067.531967/150‑1 (MQ=60)
                                                                                                                      TGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATTTAAATTATGTAaatatcggtgcaaccaccaaaatccaataaggcagtattttgcatgtcacaaataaaacatccaacaaaaaacaagtaaaaccattaaccatttg                              >  SRR19973067.449686/1‑55 (MQ=60)
                                                                                                                       GATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGC                             <  SRR19973067.1537003/150‑1 (MQ=60)
                                                                                                                       GATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGC                             <  SRR19973067.1539365/150‑1 (MQ=60)
                                                                                                                       GATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGC                             <  SRR19973067.2183371/150‑1 (MQ=60)
                                                                                                                       GATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATACAGGATGGCAGTATGTTGGATGTCACACATAAATCCTCGTACAACCCCGATGGCAACCCAATAAACAATGGC                             >  SRR19973067.1028856/1‑150 (MQ=60)
                                                                                                                       GATGATGTGGGGATCTGGCCGCTCGGTGTTATTGGTGGGGATTTACAATATTTACAGATAGGAGCCCGCACCAAAATCCAGGATGGGAGTATGTTGGATGTCACTCATAAATACTCGTACAACCCAGATGGCAACCCAATAAACATTGGG                             >  SRR19973067.1028846/1‑150 (MQ=60)
                                                                                                                           ATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAG                         <  SRR19973067.1700143/150‑1 (MQ=60)
                                                                                                                                 GGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCATGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATTGCAACCCATTAACCATTGGCGAAGATGTCA                   <  SRR19973067.2945100/150‑1 (MQ=60)
                                                                                                                                 GGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCA                   <  SRR19973067.1799502/150‑1 (MQ=60)
                                                                                                                                      TGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTT              >  SRR19973067.1320936/1‑150 (MQ=60)
                                                                                                                                        GCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGG            <  SRR19973067.1142101/150‑1 (MQ=60)
                                                                                                                                          CGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTC          <  SRR19973067.564124/150‑1 (MQ=60)
                                                                                                                                           GCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCA         >  SRR19973067.1005569/1‑150 (MQ=60)
                                                                                                                                              CGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAA      >  SRR19973067.2074452/1‑150 (MQ=60)
                                                                                                                                               GTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAG     >  SRR19973067.577764/1‑150 (MQ=60)
                                                                                                                                               GTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAG     >  SRR19973067.2112309/1‑150 (MQ=60)
                                                                                                                                               GTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAG     >  SRR19973067.3262193/1‑150 (MQ=60)
                                                                                                                                                TTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGG    <  SRR19973067.2431355/150‑1 (MQ=60)
                                                                                                                                                TTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGG    <  SRR19973067.779092/150‑1 (MQ=60)
                                                                                                                                                 TGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGT   >  SRR19973067.1680785/1‑150 (MQ=60)
                                                                                                                                                 TGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGT   >  SRR19973067.1133329/1‑150 (MQ=60)
                                                                                                                                                  GTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTG  <  SRR19973067.720524/150‑1 (MQ=60)
                                                                                                                                                  GTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTG  >  SRR19973067.2032425/1‑150 (MQ=60)
                                                                                                                                                  GTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTG  >  SRR19973067.1347122/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
TTATTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTG  >  CP009273/3422578‑3422873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑