Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F361 I0 R1 1353 250.7 8230166 96.0% 7900959 148.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,422,726 G→T 100% V44V (GTG→GTT)  yrdA → bacterial transferase hexapeptide domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,422,7260GT96.6% 778.2 / 14.5 252V44V (GTG→GTT) yrdAbacterial transferase hexapeptide domain protein
Reads supporting (aligned to +/- strand):  ref base G (3/6);  new base T (111/132);  total (114/138)
Fisher's exact test for biased strand distribution p-value = 5.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 252 total aligned reads displayed.

TATTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAG  >  CP009273/3422579‑3422870
                                                                                                                                                   |                                                                                                                                                
  ttGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTAtt                                                                                                                                               <  2:1457855/149‑1 (MQ=255)
  ttGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTAtt                                                                                                                                               <  2:1457987/149‑1 (MQ=255)
  ttGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTAtt                                                                                                                                               <  1:3626837/149‑1 (MQ=255)
      tGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTg                                                                                                                                           >  1:2731420/1‑149 (MQ=255)
         ggTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGgag                                                                                                                                        >  2:2090968/1‑149 (MQ=255)
          gTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGgaga                                                                                                                                       <  1:1861402/149‑1 (MQ=255)
           tAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGAt                                                                                                                                      <  1:803483/149‑1 (MQ=255)
           tAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGAt                                                                                                                                      >  2:2189023/1‑149 (MQ=255)
           tAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGAt                                                                                                                                      >  2:1124033/1‑149 (MQ=255)
            aaGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATg                                                                                                                                     <  1:1135695/149‑1 (MQ=255)
             aGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGGTGTTATTCGTGGAGATGt                                                                                                                                    >  1:4054898/1‑149 (MQ=255)
               tATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGttc                                                                                                                                  <  2:1325340/149‑3 (MQ=255)
               tATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGTTTCGTGGAGATGTaa                                                                                                                                  >  1:1407444/1‑148 (MQ=255)
               tATGTCGGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTAc                                                                                                                                  <  2:2895214/149‑1 (MQ=255)
                aTGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACa                                                                                                                                 <  2:1204031/149‑1 (MQ=255)
                 tGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACAt                                                                                                                                <  1:1960519/149‑1 (MQ=255)
                  gTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACAtt                                                                                                                               <  1:2633038/149‑1 (MQ=255)
                   tCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTCTTCGGGGAGATGTACATTa                                                                                                                              >  2:474414/1‑149 (MQ=255)
                   tCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTa                                                                                                                              <  2:2300186/149‑1 (MQ=255)
                     tGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATg                                                                                                                            >  1:3896553/1‑149 (MQ=255)
                           tttACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGa                                                                                                                      <  1:2780519/149‑1 (MQ=255)
                           tttACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGa                                                                                                                      <  1:153316/149‑1 (MQ=255)
                            ttACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGAt                                                                                                                     <  1:1019712/149‑1 (MQ=255)
                            ttACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGAt                                                                                                                     >  1:1979031/1‑149 (MQ=255)
                             tACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATc                                                                                                                    >  1:970814/1‑149 (MQ=255)
                             tACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATc                                                                                                                    >  2:893903/1‑149 (MQ=255)
                              aCGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCg                                                                                                                   <  2:1430652/149‑1 (MQ=255)
                                    aTACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGGTTTTAGTGGAGATGTACATTATGGACAGATCGGAgcac                                                                                                             >  1:796131/1‑149 (MQ=255)
                                     tACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAgcacg                                                                                                            >  2:2740580/1‑149 (MQ=255)
                                     tACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGGTGTTTTTAGTGGTGATTTTAAATATTTTCAGATAGGTgcacg                                                                                                            >  1:696928/1‑149 (MQ=255)
                                        cgcgATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAgcacgcac                                                                                                         <  1:375604/149‑1 (MQ=255)
                                         gcgATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCAcc                                                                                                        <  2:3662060/149‑1 (MQ=255)
                                           gATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCaa                                                                                                      >  1:296583/1‑149 (MQ=255)
                                           gATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCaa                                                                                                      <  1:2201422/149‑1 (MQ=255)
                                           gATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGATATGTAAATTATGTACAGATCGGAGCACGCACCaa                                                                                                      >  1:1205715/1‑149 (MQ=255)
                                            aTCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGGGATTTACATTATGTACAGATAGGTGCACGCACCaaa                                                                                                     >  1:51596/1‑148 (MQ=255)
                                              cTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAatat                                                                                                   <  2:2927628/149‑1 (MQ=255)
                                              cTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAatat                                                                                                   <  2:2927648/149‑1 (MQ=255)
                                              cTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAatat                                                                                                   <  1:3366567/149‑1 (MQ=255)
                                                ttttCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATcc                                                                                                 <  1:709389/149‑1 (MQ=255)
                                                 tttCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCa                                                                                                <  2:3826740/149‑1 (MQ=255)
                                                  ttCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAg                                                                                               <  1:861542/149‑1 (MQ=255)
                                                   tCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAgg                                                                                              >  2:3593415/1‑149 (MQ=255)
                                                    ccACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGa                                                                                             >  2:3067825/1‑149 (MQ=255)
                                                      acaAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACAATATGTACAGATCGGAGCACGCACCCATATCCAGGATg                                                                                           >  2:10900/1‑149 (MQ=255)
                                                       caAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATgg                                                                                          <  1:1674985/149‑1 (MQ=255)
                                                        aaaTCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGc                                                                                         <  2:810649/149‑1 (MQ=255)
                                                          aTCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAg                                                                                       <  2:778869/149‑1 (MQ=255)
                                                          aTCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAg                                                                                       <  2:2840603/149‑1 (MQ=255)
                                                          aTCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAg                                                                                       >  2:515203/1‑149 (MQ=255)
                                                          aTCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATGTAAAATATTTACAGATCGGAGCAAGCACCAAAATACACGATTGccg                                                                                       >  1:3800209/1‑147 (MQ=255)
                                                            cGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTa                                                                                     <  1:2683183/149‑1 (MQ=255)
                                                               tCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGAATTAAATTATTTACACATAGGAGCACGCACCAAAAAACAGGATGGCAGTATGt                                                                                  >  1:2907795/1‑149 (MQ=255)
                                                                    gcgTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATGTAAATTATGTTAATATAGGAGCACGCACCAATATACCGGATGGCAGTATGtttgat                                                                             >  1:385331/1‑145 (MQ=255)
                                                                      gTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGt                                                                           >  2:2767747/1‑149 (MQ=255)
                                                                           gATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTc                                                                      >  1:3696341/1‑149 (MQ=255)
                                                                           gATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTc                                                                      <  1:2159880/149‑1 (MQ=255)
                                                                            aTCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCa                                                                     >  2:1645188/1‑149 (MQ=255)
                                                                               gacgaTAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATaa                                                                  <  2:3739439/149‑1 (MQ=255)
                                                                                   aTAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATcc                                                              <  1:2540960/149‑1 (MQ=255)
                                                                                   aTAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATcc                                                              <  2:3164186/149‑1 (MQ=255)
                                                                                    tAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTTTTTTTAGTGGAGATTTTAAATATTTTAACATAGGAGCACGCACCCAAATAAAAGATGGCAGTATTTTTCATTTGAAACATaaaaaca                                                             >  1:3445949/1‑145 (MQ=255)
                                                                                     aGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTc                                                            <  1:1632805/149‑1 (MQ=255)
                                                                                        aGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATTTACAGATCGGAGCACGCACCAATATACAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTa                                                         >  1:3371382/1‑149 (MQ=255)
                                                                                        aGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATTTAAAATATGTACACATCCGAGCACGCACCAAAAACCACGATGGCAGTATGTTGCAAGTCACTCAAAAAACCTCGTa                                                         >  1:3326084/1‑149 (MQ=255)
                                                                                              gTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAAccc                                                   >  1:888705/1‑149 (MQ=255)
                                                                                                  ttGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGAt                                               <  2:4068744/149‑1 (MQ=255)
                                                                                                  ttGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGAt                                               <  1:1092947/149‑1 (MQ=255)
                                                                                                   tGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATg                                              <  1:1597003/149‑1 (MQ=255)
                                                                                                   tGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATg                                              <  1:1597069/149‑1 (MQ=255)
                                                                                                     gTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGc                                            <  1:1124033/149‑1 (MQ=255)
                                                                                                           ttCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATAGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAAACCTCGTACAACCCAGATGGCAAcccc                                      >  1:2989776/1‑148 (MQ=255)
                                                                                                           ttCGTCTGGCTGATGATGTGGGGATCTGGCCGCGCGGTGTTATTAGTGGAGATTTTAAATATTTAAATATCGGAGCACGCACCAAAATACACGATGGCAGTATGTTGGATGTCACACATAAATACACGTACAAACCAGATTGCAACCCa                                      >  1:247/1‑149 (MQ=255)
                                                                                                             cGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCAtt                                    <  2:250628/149‑1 (MQ=255)
                                                                                                             cGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCAtt                                    <  2:3526335/149‑1 (MQ=255)
                                                                                                               tCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTaa                                  <  2:1588544/149‑1 (MQ=255)
                                                                                                               tCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTaa                                  <  2:1808299/149‑1 (MQ=255)
                                                                                                               tCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTaa                                  <  1:2304661/149‑1 (MQ=255)
                                                                                                                cTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAAc                                 <  1:58537/149‑1 (MQ=255)
                                                                                                                cTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAAc                                 <  1:23345/149‑1 (MQ=255)
                                                                                                                 tGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAAcc                                >  1:1337891/1‑149 (MQ=255)
                                                                                                                     tgatgaTGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACATATAGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTg                            >  2:2075259/1‑149 (MQ=255)
                                                                                                                     tgatgaTGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTg                            <  1:2200977/149‑1 (MQ=255)
                                                                                                                      gatgatGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGTGATGTACATTATGTAAAGATCGGAGCACGCACCAAAATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATACTCGTACAACCCACATGGCAACCCATTAACCATTgg                           >  1:3877369/1‑149 (MQ=255)
                                                                                                                      gatgatGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGTAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTgg                           <  2:1758263/149‑1 (MQ=255)
                                                                                                                      gatgatGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGTAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTgg                           <  2:3798522/149‑1 (MQ=255)
                                                                                                                      gatgatGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTgg                           >  2:3365338/1‑149 (MQ=255)
                                                                                                                          atgTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGaa                       <  2:312808/149‑1 (MQ=255)
                                                                                                                            gtgGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGa                     <  1:1819337/149‑1 (MQ=255)
                                                                                                                              ggggATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATg                   <  2:1730775/149‑1 (MQ=255)
                                                                                                                                 gATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCa                <  1:3258515/149‑1 (MQ=255)
                                                                                                                                 gATCTGGCCGCTCGTTGTTATTCGGGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATACTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCa                >  2:2222409/1‑149 (MQ=255)
                                                                                                                                  aTCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCAc               <  1:2090938/149‑1 (MQ=255)
                                                                                                                                     tGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGt            >  2:4022684/1‑149 (MQ=255)
                                                                                                                                     tGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTAAAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGt            >  2:1165624/1‑149 (MQ=255)
                                                                                                                                      ggCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGtt           <  2:2520301/149‑1 (MQ=255)
                                                                                                                                        ccGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTgg         <  1:4080384/149‑1 (MQ=255)
                                                                                                                                             cGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTcaca    >  2:1812509/1‑149 (MQ=255)
                                                                                                                                               ttGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAg  <  2:1029656/149‑1 (MQ=255)
                                                                                                                                               ttGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAg  >  1:454258/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TATTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAG  >  CP009273/3422579‑3422870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTATTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGAT  >  CP009273/3422577‑3422875
                                                                                                                                                     |                                                                                                                                                     
TTTATTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTT                                                                                                                                                       <  SRR19973068.1075902/150‑1 (MQ=60)
TTTATTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTT                                                                                                                                                       <  SRR19973068.1076256/150‑1 (MQ=60)
TTTATTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTT                                                                                                                                                       <  SRR19973068.3248360/150‑1 (MQ=60)
  TATTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTAT                                                                                                                                                     <  SRR19973068.1322158/150‑1 (MQ=60)
  TATTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGAT                                                                                                                                                     <  SRR19973068.74043/150‑1 (MQ=60)
    TTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTC                                                                                                                                                   <  SRR19973068.3648834/150‑1 (MQ=60)
       CTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTG                                                                                                                                                >  SRR19973068.2748269/1‑150 (MQ=60)
         GAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGA                                                                                                                                              <  SRR19973068.2184859/150‑1 (MQ=60)
          AGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCGCGGTGTTATTCGTGGAG                                                                                                                                             >  SRR19973068.3810256/1‑150 (MQ=60)
           GGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGA                                                                                                                                            >  SRR19973068.2960235/1‑150 (MQ=60)
            GTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGAT                                                                                                                                           <  SRR19973068.1873708/150‑1 (MQ=60)
             TAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATT                                                                                                                                          >  SRR19973068.3868695/1‑150 (MQ=60)
             TAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATG                                                                                                                                          <  SRR19973068.808949/150‑1 (MQ=60)
              AAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGT                                                                                                                                         <  SRR19973068.1143284/150‑1 (MQ=60)
              AAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGT                                                                                                                                         <  SRR19973068.1143275/150‑1 (MQ=60)
              AAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGGTGTTATTCGTGGAGATGT                                                                                                                                         >  SRR19973068.4079293/1‑150 (MQ=60)
                GTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGTTTCGTGGAGATGTAA                                                                                                                                       >  SRR19973068.1416517/1‑150 (MQ=60)
                   TGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATT                                                                                                                                    <  SRR19973068.508152/150‑1 (MQ=60)
                   TGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATT                                                                                                                                    <  SRR19973068.1973525/150‑1 (MQ=60)
                    GTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTA                                                                                                                                   <  SRR19973068.2649587/150‑1 (MQ=60)
                      CTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATG                                                                                                                                 >  SRR19973068.3919901/1‑150 (MQ=60)
                      CTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATG                                                                                                                                 >  SRR19973068.3920119/1‑150 (MQ=60)
                       TGATGTTTTACGCCCATACCGCGATCTTTTTACACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGT                                                                                                                                <  SRR19973068.4106395/150‑1 (MQ=60)
                        GATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTA                                                                                                                               <  SRR19973068.1902902/150‑1 (MQ=60)
                        GATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTA                                                                                                                               <  SRR19973068.2379420/150‑1 (MQ=60)
                             TTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGAT                                                                                                                          <  SRR19973068.154616/150‑1 (MQ=60)
                             TTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGAT                                                                                                                          >  SRR19973068.1990645/1‑150 (MQ=60)
                             TTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGAT                                                                                                                          >  SRR19973068.1992123/1‑150 (MQ=60)
                             TTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGAT                                                                                                                          <  SRR19973068.2797712/150‑1 (MQ=60)
                             TTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGAT                                                                                                                          <  SRR19973068.1377265/150‑1 (MQ=60)
                             TTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATGTACATTATGTACAGAT                                                                                                                          >  SRR19973068.1992167/1‑150 (MQ=60)
                              TTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATC                                                                                                                         <  SRR19973068.1026328/150‑1 (MQ=60)
                              TTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATC                                                                                                                         >  SRR19973068.977259/1‑150 (MQ=60)
                               TACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCG                                                                                                                        <  SRR19973068.1214951/150‑1 (MQ=60)
                                  GCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAG                                                                                                                     <  SRR19973068.2564165/150‑1 (MQ=60)
                                  GCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAG                                                                                                                     <  SRR19973068.4080861/150‑1 (MQ=60)
                                     CATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTTTTATTCGTGGTGATTTACAATATGTACAAATAGGAGCAA                                                                                                                  >  SRR19973068.802944/1‑150 (MQ=60)
                                     CATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGGTTTTAGTGGAGATGTACATTATGGACAGATCGGAGCAC                                                                                                                  >  SRR19973068.801546/1‑150 (MQ=60)
                                          CGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACC                                                                                                             <  SRR19973068.378158/150‑1 (MQ=60)
                                          CGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGTGATGTAAAATATTTACAGATCGGAGCACGCACC                                                                                                             >  SRR19973068.792233/1‑150 (MQ=60)
                                            CGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAA                                                                                                           >  SRR19973068.298569/1‑150 (MQ=60)
                                            CGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGATATGTAAATTATGTACAGATCGGAGCACGCACCAA                                                                                                           >  SRR19973068.1213760/1‑150 (MQ=60)
                                             GATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAAT                                                                                                          <  SRR19973068.2215806/150‑1 (MQ=60)
                                             GATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGGGATTTACATTATGTACAGATAGGTGCACGCACCAAA                                                                                                          >  SRR19973068.52216/1‑150 (MQ=60)
                                               TCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATAT                                                                                                        <  SRR19973068.3002379/150‑1 (MQ=60)
                                                CTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATTTAAATATAGGAGCACGCACCAATATA                                                                                                       >  SRR19973068.2552823/1‑150 (MQ=60)
                                                CTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATC                                                                                                       <  SRR19973068.3387027/150‑1 (MQ=60)
                                                  TTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCA                                                                                                     <  SRR19973068.747069/150‑1 (MQ=60)
                                                  TTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCA                                                                                                     <  SRR19973068.713967/150‑1 (MQ=60)
                                                  TTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCA                                                                                                     <  SRR19973068.713917/150‑1 (MQ=60)
                                                    TTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGG                                                                                                   <  SRR19973068.867394/150‑1 (MQ=60)
                                                        ACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGG                                                                                               <  SRR19973068.45592/150‑1 (MQ=60)
                                                         CAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGC                                                                                              <  SRR19973068.1685807/150‑1 (MQ=60)
                                                         CAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGC                                                                                              >  SRR19973068.3401057/1‑150 (MQ=60)
                                                         CAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGC                                                                                              <  SRR19973068.1685781/150‑1 (MQ=60)
                                                           AATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATGTAAAATATTTACAGATCGGAGCAAGCACCAAaatacacgattgccg                                                                                            >  SRR19973068.3823000/1‑135 (MQ=60)
                                                              CGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTAT                                                                                         <  SRR19973068.1952131/150‑1 (MQ=60)
                                                              CGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTAT                                                                                         <  SRR19973068.2699892/150‑1 (MQ=60)
                                                               GGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATG                                                                                        <  SRR19973068.1865000/150‑1 (MQ=60)
                                                               GGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAAAATACAGGATTGCAGTATG                                                                                        >  SRR19973068.1601392/1‑150 (MQ=60)
                                                                GTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGAATTAAATTATTTACACATAGGAGCACGCACCAAAAAACAGGATGGCAGTATGT                                                                                       >  SRR19973068.2925887/1‑150 (MQ=60)
                                                                GTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGGTGTTATTAGTGGGGATGTTCATTATTTAAAGATCGGAGCAAGCCCCAAAATCCACGATGGGAGTATGT                                                                                       >  SRR19973068.39867/1‑150 (MQ=60)
                                                                     CGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATGTAAATTATTTAAAGATAGGAGCACGCACCAAAATCCAGGATGGCAGTATGTTGCAT                                                                                  >  SRR19973068.1203853/1‑150 (MQ=60)
                                                                     CGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATGTAAATTATGTTAATATAGGAGCACGCACCAATATACCGGATGGCAGTATGTTtgat                                                                                  >  SRR19973068.387989/1‑146 (MQ=60)
                                                                     CGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCGCGTTGTTATTCGTGGAGATGTAAAGTATGTACATATCGGAGCACGCACCAAAATCCAGGATGGCAGGATGTTTCAT                                                                                  >  SRR19973068.1617961/1‑150 (MQ=60)
                                                                     CGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCGCGGTGTTATTCGTGGAGATTTAAAATATGTACAGATAGGAGCACCCACCAATATACAGGATGGCAGTATTTTGCAT                                                                                  >  SRR19973068.1203866/1‑150 (MQ=60)
                                                                       CGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGT                                                                                <  SRR19973068.2648058/150‑1 (MQ=60)
                                                                         TAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCCAAATCCAGGATGGCAGTATGTTGCATGTCA                                                                              >  SRR19973068.3115317/1‑150 (MQ=60)
                                                                          AATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCAC                                                                             <  SRR19973068.165142/150‑1 (MQ=60)
                                                                           ATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTTGTGATGTACATTATGTACAGATCGGAGCACGCACCAATATACACGATGGCAGTATTTTTCATGTCACT                                                                            >  SRR19973068.1266559/1‑150 (MQ=60)
                                                                            TGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTC                                                                           >  SRR19973068.3718607/1‑150 (MQ=60)
                                                                            TGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATTTACAATATTTACAGATCtgatcacgccccaaaatacaggaatgcagtatttttcatttaactc                                                                           >  SRR19973068.836419/1‑104 (MQ=60)
                                                                             GATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCA                                                                          <  SRR19973068.2174029/150‑1 (MQ=60)
                                                                             GATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTTCATGTCACACA                                                                          >  SRR19973068.1575657/1‑150 (MQ=60)
                                                                             GATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCA                                                                          <  SRR19973068.3718162/150‑1 (MQ=60)
                                                                                   CGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATTTACATTATGTACAGATAGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCAAAAATA                                                                    >  SRR19973068.2652710/1‑150 (MQ=60)
                                                                                   CGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATTTAAAGATCGGAGCACGCACCAAAATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATC                                                                    >  SRR19973068.1027193/1‑150 (MQ=60)
                                                                                     ATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCT                                                                  <  SRR19973068.2557096/150‑1 (MQ=60)
                                                                                       AGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCG                                                                <  SRR19973068.1643418/150‑1 (MQ=60)
                                                                                       AGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCG                                                                <  SRR19973068.1643450/150‑1 (MQ=60)
                                                                                         CAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATTTACAGATCGGAGCACGCACCAATATACAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTA                                                              >  SRR19973068.3391857/1‑150 (MQ=60)
                                                                                         CAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTA                                                              <  SRR19973068.2973339/150‑1 (MQ=60)
                                                                                         CAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATAGGAGCACGCACCAAAATCCAGGATGGCAGTATGTTGCATGTCACTCATAAAaactagta                                                              >  SRR19973068.3391552/1‑142 (MQ=60)
                                                                                         CAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTAAAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATTTTGCATTTCACTCATAAATACACGTA                                                              >  SRR19973068.3391595/1‑150 (MQ=60)
                                                                                         CAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATTTAAAATATGTACACATCCGAGCACGCACCAAAAACCACGATGGCAGTATGTTGCAAGTCACTCAAAAAACCTCGTA                                                              >  SRR19973068.3346393/1‑150 (MQ=60)
                                                                                             GTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTTTTATTCGTGGAGATTTAAATTATTTAAATATCGGAGCACCCACCAAAATCCACGATGGCAGTATTTTGCATGTCACTCATAAATACTCGTACAAC                                                          >  SRR19973068.1510372/1‑150 (MQ=60)
                                                                                             GTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAAC                                                          <  SRR19973068.1105057/150‑1 (MQ=60)
                                                                                               CGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATTTACATTATGTACAGATCGGAGCACCCACCAATATCCAGGATGGCAGTATGTTTCATGTCACTCATAAAACCTCGTACAACCC                                                        >  SRR19973068.894588/1‑150 (MQ=60)
                                                                                               CGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATACAGGATGGCAGTATTTTGCATGTCACTCATAAATCCTCGTACAACCC                                                        >  SRR19973068.894718/1‑150 (MQ=60)
                                                                                                GTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCGCGTTGTTATTAGTGGTGATGTAAAATATGTAAATATCGGAGCACGCACCAAAATCCAGGATTGGAGTATGTTGGATGTAACTAATAAATACTCGTACAACCCA                                                       >  SRR19973068.1771320/1‑150 (MQ=60)
                                                                                                    TTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATG                                                   <  SRR19973068.1100212/150‑1 (MQ=60)
                                                                                                     TGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGG                                                  <  SRR19973068.1607533/150‑1 (MQ=60)
                                                                                                     TGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGG                                                  <  SRR19973068.1607467/150‑1 (MQ=60)
                                                                                                     TGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGG                                                  <  SRR19973068.1607530/150‑1 (MQ=60)
                                                                                                     TGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGG                                                  <  SRR19973068.2769699/150‑1 (MQ=60)
                                                                                                       GTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCA                                                <  SRR19973068.1130862/150‑1 (MQ=60)
                                                                                                       GTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCA                                                <  SRR19973068.1131545/150‑1 (MQ=60)
                                                                                                        TGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAA                                               <  SRR19973068.685546/150‑1 (MQ=60)
                                                                                                            GTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATAGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAAACCTCGTACAACCCAGATGGCAACCCC                                           >  SRR19973068.3008262/1‑150 (MQ=60)
                                                                                                            GTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCGCGGTGTTATTAGTGGAGATTTTAAATATTTAAATATCGGAGCACGCACCAAAATACACGATGGCAGTATGTTGGATGTCACACATAAATACACGTACAAACCAGATTGCAACCCA                                           >  SRR19973068.249/1‑150 (MQ=38)
                                                                                                             TTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAAAATACAGGATGGCAGTATGTTGCATGTCACTCATAAATACACGTACAACCCAGATTGCAACCCAT                                          >  SRR19973068.949698/1‑150 (MQ=60)
                                                                                                             TTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGGGGAGATTTACAATATGTACAGATCGGAGCACGCACCAAAATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATACTCGTACAACCCAGATGGCAACCCAA                                          >  SRR19973068.949714/1‑150 (MQ=60)
                                                                                                              TCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATT                                         <  SRR19973068.2702317/150‑1 (MQ=60)
                                                                                                               CGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTA                                        <  SRR19973068.867247/150‑1 (MQ=60)
                                                                                                                 TCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAAC                                      <  SRR19973068.2319670/150‑1 (MQ=60)
                                                                                                                 TCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAAC                                      <  SRR19973068.2319548/150‑1 (MQ=60)
                                                                                                                 TCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAAC                                      <  SRR19973068.3370494/150‑1 (MQ=60)
                                                                                                                  CTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACC                                     <  SRR19973068.59229/150‑1 (MQ=60)
                                                                                                                  CTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACC                                     <  SRR19973068.23683/150‑1 (MQ=60)
                                                                                                                  CTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACC                                     <  SRR19973068.58075/150‑1 (MQ=60)
                                                                                                                  CTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACC                                     >  SRR19973068.1346544/1‑150 (MQ=60)
                                                                                                                      CTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAAACCTCGTACAACCCACATGGCAACCCAATAACCATTG                                 >  SRR19973068.3370558/1‑150 (MQ=60)
                                                                                                                       TGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGTGATGTACATTATGTAAAGATCGGAGCACGCACCAAAATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATACTCGTACAACCCACATGGCAACCCATTAACCATTGG                                >  SRR19973068.3900780/1‑150 (MQ=60)
                                                                                                                       TGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGG                                <  SRR19973068.2215363/150‑1 (MQ=60)
                                                                                                                       TGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGG                                <  SRR19973068.2215357/150‑1 (MQ=60)
                                                                                                                       TGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGG                                <  SRR19973068.2203345/150‑1 (MQ=60)
                                                                                                                       TGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATAGGAGCACGCACCAAAATCCAGGATGGCAGTATGTTTCATGTCACTCATAAATCCACGTACAACCCACATGGCAACCCAATAACCAATGG                                >  SRR19973068.3900776/1‑150 (MQ=60)
                                                                                                                        GATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGC                               <  SRR19973068.3151082/150‑1 (MQ=60)
                                                                                                                           GATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCAATAACCATTGGCGAA                            >  SRR19973068.3791760/1‑150 (MQ=60)
                                                                                                                            ATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAG                           <  SRR19973068.3528314/150‑1 (MQ=60)
                                                                                                                            ATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAG                           <  SRR19973068.3735806/150‑1 (MQ=60)
                                                                                                                            ATGTGGGGATCGGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAG                           <  SRR19973068.3943938/150‑1 (MQ=60)
                                                                                                                             TGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTAAATTATGTACAGATAGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAAACCAGATGGCAACCCATTAACCATTTGCGAAGA                          >  SRR19973068.569143/1‑150 (MQ=60)
                                                                                                                              GTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGAT                         <  SRR19973068.1831294/150‑1 (MQ=60)
                                                                                                                              GTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGAT                         <  SRR19973068.3883188/150‑1 (MQ=60)
                                                                                                                               TGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAAACCTCGTACAAACCAGATGGCAACCCATTAACCATTGGCGAAGATG                        >  SRR19973068.244161/1‑150 (MQ=60)
                                                                                                                               TGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATGTACATTATGTACAGATAGGAGCACGCACCAATATCCAGGATGGCAGTATTTTGCATGTAACTCATAAATCCTCGTACAACCCAGAAGGCAACCCAATAAACAATGGCGAAGATG                        >  SRR19973068.3691052/1‑150 (MQ=60)
                                                                                                                                  GGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCA                     <  SRR19973068.2293547/150‑1 (MQ=60)
                                                                                                                                  GGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCA                     <  SRR19973068.2294019/150‑1 (MQ=60)
                                                                                                                                   GATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCAC                    <  SRR19973068.3278532/150‑1 (MQ=60)
                                                                                                                                    ATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACT                   <  SRR19973068.2104564/150‑1 (MQ=60)
                                                                                                                                    ATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACT                   <  SRR19973068.2104534/150‑1 (MQ=60)
                                                                                                                                      CTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGT                 <  SRR19973068.2655213/150‑1 (MQ=60)
                                                                                                                                        GGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTG               >  SRR19973068.3313566/1‑150 (MQ=60)
                                                                                                                                          CCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGT             <  SRR19973068.4104862/150‑1 (MQ=60)
                                                                                                                                           CGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTC            <  SRR19973068.2757460/150‑1 (MQ=60)
                                                                                                                                                GTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAG       >  SRR19973068.457475/1‑150 (MQ=60)
                                                                                                                                                GTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAG       >  SRR19973068.2921849/1‑150 (MQ=60)
                                                                                                                                                GTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAG       >  SRR19973068.2054578/1‑150 (MQ=60)
                                                                                                                                                   GTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTG    >  SRR19973068.2589212/1‑150 (MQ=60)
                                                                                                                                                     TATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCGCAAGGTGAT  <  SRR19973068.1219189/150‑1 (MQ=60)
                                                                                                                                                     TATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGAT  <  SRR19973068.1219131/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TTTATTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGAT  >  CP009273/3422577‑3422875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑