Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,422,726 G→T 100% V44V (GTG→GTT)  yrdA → bacterial transferase hexapeptide domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,422,7260GT97.9% 610.4 / 3.7 192V44V (GTG→GTT) yrdAbacterial transferase hexapeptide domain protein
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base T (77/111);  total (78/114)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 195 total aligned reads displayed.

TTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAG  >  CP009273/3422581‑3422870
                                                                                                                                                 |                                                                                                                                                
ttGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTAt                                                                                                                                                <  1:2905945/148‑1 (MQ=255)
  gCTGAGGTATGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGTTTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTc                                                                                                                                              <  1:2226919/148‑1 (MQ=255)
  gCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTc                                                                                                                                              >  2:2568543/1‑148 (MQ=255)
     gAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTg                                                                                                                                           <  1:3002944/148‑1 (MQ=255)
        gTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGgag                                                                                                                                        <  2:2271216/148‑1 (MQ=255)
        gTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGgag                                                                                                                                        >  1:1639169/1‑148 (MQ=255)
             tATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTa                                                                                                                                   >  1:3511048/1‑148 (MQ=255)
              aTGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTAc                                                                                                                                  <  2:1167966/148‑1 (MQ=255)
                    gATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATg                                                                                                                            >  1:2137303/1‑148 (MQ=255)
                       gTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTAc                                                                                                                         <  2:1295418/148‑1 (MQ=255)
                       gTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTAc                                                                                                                         <  1:415587/148‑1 (MQ=255)
                       gTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTAc                                                                                                                         <  1:2183188/148‑1 (MQ=255)
                         tttACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAg                                                                                                                       >  2:2904968/1‑148 (MQ=255)
                            aCGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATc                                                                                                                    >  1:1408791/1‑148 (MQ=255)
                            aCGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGGTGTTATTCGTGGAGATGTACATTATGTACAGATc                                                                                                                    >  2:1742661/1‑148 (MQ=255)
                              gCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCgg                                                                                                                  <  2:1921793/148‑1 (MQ=255)
                                 cATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCGCGGTGTTATTCGTGGAGATGTACATTATGTAAAGATCGGAgc                                                                                                               >  1:53002/1‑148 (MQ=255)
                                  aTACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAgca                                                                                                              <  1:410465/148‑1 (MQ=255)
                                    aCCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAgcacg                                                                                                            >  2:206151/1‑148 (MQ=255)
                                     ccGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAgcacgc                                                                                                           <  2:2509638/148‑1 (MQ=255)
                                           tCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAat                                                                                                     >  2:3243571/1‑148 (MQ=255)
                                           tCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAat                                                                                                     >  2:2429748/1‑148 (MQ=255)
                                           tCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGGTGTTATTCGTGGAGATGTAAATTATGTTCAGATAGGAGCACGCACCAat                                                                                                     >  1:2418964/1‑148 (MQ=255)
                                             tttttCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAatat                                                                                                   <  1:2714362/148‑1 (MQ=255)
                                                ttCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCa                                                                                                <  2:2896543/148‑1 (MQ=255)
                                                  ccACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAgg                                                                                              <  2:1458475/148‑1 (MQ=255)
                                                  ccACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAgg                                                                                              <  2:1206672/148‑1 (MQ=255)
                                                    acaAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATTTACATTATGTACAGATAGGAGCACGCACCAATATACAGGAt                                                                                            >  1:3009870/1‑148 (MQ=255)
                                                      aaaTCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATgg                                                                                          <  1:1253588/148‑1 (MQ=255)
                                                       aaTCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATTGc                                                                                         >  2:2665439/1‑148 (MQ=255)
                                                       aaTCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGc                                                                                         <  1:2445682/148‑1 (MQ=255)
                                                         tCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAg                                                                                       >  2:3507755/1‑148 (MQ=255)
                                                         tCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGGTGTTATTCGTGGAGATGTACATTATGTAAAGATCGGAGCACGCACCAATATCCAGGATGGCAg                                                                                       >  1:2036928/1‑148 (MQ=255)
                                                          cGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATACAGGATGGCAGt                                                                                      >  2:3365430/1‑148 (MQ=255)
                                                           ggTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTa                                                                                     <  1:3243571/148‑1 (MQ=255)
                                                             tCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATg                                                                                   >  2:1165944/1‑148 (MQ=255)
                                                               aGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGtt                                                                                 >  2:2952667/1‑148 (MQ=255)
                                                                  gcgTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCa                                                                              <  2:2713619/148‑1 (MQ=255)
                                                                   cgTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCAt                                                                             >  2:765482/1‑148 (MQ=255)
                                                                     tAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGt                                                                           <  1:1873150/148‑1 (MQ=255)
                                                                     tAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGt                                                                           <  1:588522/148‑1 (MQ=255)
                                                                       aTGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGGTGTTATTCGTGGAGATGTACATTATGTACCGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCa                                                                         >  2:2938577/1‑148 (MQ=255)
                                                                         gATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACt                                                                       >  2:1966507/1‑148 (MQ=255)
                                                                          aTCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTc                                                                      <  2:269144/148‑1 (MQ=255)
                                                                           tCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCa                                                                     >  2:422772/1‑148 (MQ=255)
                                                                           tCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCa                                                                     <  2:840933/148‑1 (MQ=255)
                                                                             gacgaTAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATa                                                                   <  2:3120685/148‑1 (MQ=255)
                                                                              acgaTAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATaa                                                                  <  1:756535/148‑1 (MQ=255)
                                                                              acgaTAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATaa                                                                  <  1:765482/148‑1 (MQ=255)
                                                                              acgaTAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATaa                                                                  >  1:1169127/1‑148 (MQ=255)
                                                                                gaTAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAAt                                                                >  1:1992447/1‑148 (MQ=255)
                                                                                 aTAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATc                                                               >  2:1035514/1‑148 (MQ=255)
                                                                                 aTAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATc                                                               >  2:1393932/1‑148 (MQ=255)
                                                                                  tAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATcc                                                              <  2:1639169/148‑1 (MQ=255)
                                                                                  tAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATcc                                                              <  1:373129/148‑1 (MQ=255)
                                                                                   aGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCt                                                             <  2:2781075/148‑1 (MQ=255)
                                                                                    gCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTc                                                            <  2:2283163/148‑1 (MQ=255)
                                                                                     cAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCg                                                           <  2:1211403/148‑1 (MQ=255)
                                                                                     cAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGGTTTTATTAGTGGGGATTTTAAATATTTAAAGATACGAGCACCCCCCAAAATAAAAGATGGGCGTATTTTGGATGTAACACAaaaaaacaac                                                           >  1:3257684/1‑142 (MQ=255)
                                                                                      aGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGt                                                          <  1:3076804/148‑1 (MQ=255)
                                                                                       gtgtCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCGGGGTGTTATTAGTGGAGATTTAAAATATTTAAAGATCGGAGCACCCACCAAAATCCAGGATGGCCGTCTGTTTCATGTCACACAAAAAAccaccta                                                         >  1:1268551/1‑143 (MQ=255)
                                                                                        tgtCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTAc                                                        >  2:280352/1‑148 (MQ=255)
                                                                                          tCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACaa                                                      <  1:1165610/148‑1 (MQ=255)
                                                                                           cGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAAc                                                     >  2:16505/1‑148 (MQ=255)
                                                                                            gTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAAcc                                                    <  1:1941687/148‑1 (MQ=255)
                                                                                            gTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAAcc                                                    <  1:1722504/148‑1 (MQ=255)
                                                                                              gATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCa                                                  >  2:3087422/1‑148 (MQ=255)
                                                                                               aTTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAg                                                 >  1:2497984/1‑148 (MQ=255)
                                                                                                ttGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCACGTACAACCCAGa                                                >  2:2080122/1‑148 (MQ=255)
                                                                                                 tGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCCATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGAt                                               >  2:489538/1‑148 (MQ=255)
                                                                                                        gTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAAcc                                        <  2:1584171/148‑1 (MQ=255)
                                                                                                          tCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCa                                      <  2:891544/148‑1 (MQ=255)
                                                                                                          tCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCa                                      <  2:891489/148‑1 (MQ=255)
                                                                                                          tCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCa                                      <  1:430712/148‑1 (MQ=255)
                                                                                                           cGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCACGTACAACCCACATGGCAACCCAt                                     >  1:2061425/1‑148 (MQ=255)
                                                                                                                ggCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGATATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAAcc                                <  1:2383178/148‑1 (MQ=255)
                                                                                                                ggCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAAcc                                <  2:482224/148‑1 (MQ=255)
                                                                                                                 gCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCa                               <  1:1469711/148‑1 (MQ=255)
                                                                                                                 gCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAAAATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCCTTAAccc                               >  2:2093704/1‑147 (MQ=255)
                                                                                                                  cTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCAt                              >  2:2565824/1‑148 (MQ=255)
                                                                                                                  cTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCAt                              <  2:161873/148‑1 (MQ=255)
                                                                                                                  cTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCAt                              <  2:1494129/148‑1 (MQ=255)
                                                                                                                  cTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCAt                              <  1:2411055/148‑1 (MQ=255)
                                                                                                                    gatgatGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTg                            <  1:3446619/148‑1 (MQ=255)
                                                                                                                    gatgatGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTg                            <  2:3101480/148‑1 (MQ=255)
                                                                                                                    gatgatGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTg                            <  2:488365/148‑1 (MQ=255)
                                                                                                                      tgatgTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGc                          <  2:2418425/148‑1 (MQ=255)
                                                                                                                            ggggATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATTGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGAt                    <  2:540502/148‑1 (MQ=255)
                                                                                                                             gggATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATg                   >  1:3462489/1‑148 (MQ=255)
                                                                                                                                aTCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCa                <  1:1003/148‑1 (MQ=255)
                                                                                                                                aTCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCa                <  1:851211/148‑1 (MQ=255)
                                                                                                                                  cTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACt              <  1:2692676/148‑1 (MQ=255)
                                                                                                                                   tGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTg             <  2:3511048/148‑1 (MQ=255)
                                                                                                                                   tGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTg             <  1:1542028/148‑1 (MQ=255)
                                                                                                                                    ggCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGt            >  2:391522/1‑148 (MQ=255)
                                                                                                                                    ggCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGt            >  2:1934974/1‑148 (MQ=255)
                                                                                                                                         cTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTc       <  1:1026811/148‑1 (MQ=255)
                                                                                                                                          tCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTca      >  1:1687433/1‑148 (MQ=255)
                                                                                                                                             ttGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACaa   <  2:236449/148‑1 (MQ=255)
                                                                                                                                              tgttATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAg  >  1:2804417/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAG  >  CP009273/3422581‑3422870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTATTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGAT  >  CP009273/3422578‑3422875
                                                                                                                                                    |                                                                                                                                                     
TTATTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTA                                                                                                                                                      >  SRR19973065.238151/1‑150 (MQ=60)
 TATTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTAT                                                                                                                                                     <  SRR19973065.2259057/150‑1 (MQ=60)
   TTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTC                                                                                                                                                   <  SRR19973065.2923656/150‑1 (MQ=60)
     GCTGAGGTATGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGTTTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGT                                                                                                                                                 <  SRR19973065.2241105/150‑1 (MQ=60)
     GCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGT                                                                                                                                                 <  SRR19973065.1967292/150‑1 (MQ=60)
       tgctcttccgatcTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGG                                                                                                                                               <  SRR19973065.1494532/137‑1 (MQ=60)
        GAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGA                                                                                                                                              <  SRR19973065.3021342/150‑1 (MQ=60)
         AGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTTTTCGTGGAG                                                                                                                                             >  SRR19973065.1648022/1‑150 (MQ=60)
         AGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCGCGTTGTTATTCGTGGAG                                                                                                                                             >  SRR19973065.1650180/1‑150 (MQ=60)
         AGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCGCGTTGTTATTAGTGGAG                                                                                                                                             >  SRR19973065.413401/1‑150 (MQ=60)
              AGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTA                                                                                                                                        >  SRR19973065.3532342/1‑150 (MQ=60)
              AGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTA                                                                                                                                        <  SRR19973065.2092093/150‑1 (MQ=60)
                 ATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACAT                                                                                                                                     <  SRR19973065.2826513/150‑1 (MQ=60)
                    TCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTAT                                                                                                                                  <  SRR19973065.2579907/150‑1 (MQ=60)
                    TCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTAT                                                                                                                                  >  SRR19973065.1765485/1‑150 (MQ=60)
                     CTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGGGATGTTCATTATG                                                                                                                                 >  SRR19973065.2151054/1‑150 (MQ=60)
                        ATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTAC                                                                                                                              <  SRR19973065.3264705/150‑1 (MQ=60)
                          GTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAG                                                                                                                            <  SRR19973065.2197196/150‑1 (MQ=60)
                          GTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAG                                                                                                                            <  SRR19973065.418499/150‑1 (MQ=60)
                          GTTTTACGCCCATACCGAGATCTTTTTCCACTAATCGGTAAGGGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTATGGCTGATGATGTGGTGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAG                                                                                                                            <  SRR19973065.2197693/150‑1 (MQ=60)
                           TTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGGTGTTATTCGTGGAGATGTACATTATTTACAGA                                                                                                                           >  SRR19973065.3027523/1‑150 (MQ=60)
                            TTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGAT                                                                                                                          <  SRR19973065.602070/150‑1 (MQ=60)
                             TTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATC                                                                                                                         >  SRR19973065.1418008/1‑150 (MQ=60)
                                  CCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATGTACATTATTTACAGATAGGAGC                                                                                                                    >  SRR19973065.53698/1‑150 (MQ=60)
                                  CCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCGCGGTGTTATTCGTGGAGATGTACATTATGTAAAGATCGGAGC                                                                                                                    >  SRR19973065.53586/1‑150 (MQ=60)
                                   CCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCA                                                                                                                   <  SRR19973065.1696556/150‑1 (MQ=60)
                                     ATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACG                                                                                                                 <  SRR19973065.413339/150‑1 (MQ=60)
                                            GATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGGTGTTATTCGTGGAGATGTAAATTATGTTCAGATAGGAGCACGCACCAAT                                                                                                          >  SRR19973065.2434206/1‑150 (MQ=60)
                                              TCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATAT                                                                                                        <  SRR19973065.3373738/150‑1 (MQ=60)
                                                TTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCC                                                                                                      <  SRR19973065.2731246/150‑1 (MQ=60)
                                                     CCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATTTACATTATGTACAGATAGGAGCACGCACCAATATACAGGAT                                                                                                 >  SRR19973065.3028303/1‑150 (MQ=60)
                                                         AAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCA                                                                                             <  SRR19973065.1261843/150‑1 (MQ=60)
                                                          AATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAG                                                                                            <  SRR19973065.2461091/150‑1 (MQ=60)
                                                          AATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAG                                                                                            <  SRR19973065.2460841/150‑1 (MQ=60)
                                                          AATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGGTGTTATTCGTGGAGATGTACATTATGTAAAGATCGGAGCACGCACCAATATCCAGGATGGCAG                                                                                            >  SRR19973065.2049980/1‑150 (MQ=60)
                                                              GGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATG                                                                                        <  SRR19973065.3263133/150‑1 (MQ=60)
                                                                TCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTT                                                                                      <  SRR19973065.2716568/150‑1 (MQ=60)
                                                                        TAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCA                                                                              <  SRR19973065.592175/150‑1 (MQ=60)
                                                                        TAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCA                                                                              <  SRR19973065.1885548/150‑1 (MQ=60)
                                                                        TAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCA                                                                              <  SRR19973065.3473494/150‑1 (MQ=60)
                                                                        TAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCA                                                                              <  SRR19973065.422594/150‑1 (MQ=60)
                                                                        TAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCA                                                                              >  SRR19973065.3213427/1‑150 (MQ=60)
                                                                        TAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCA                                                                              <  SRR19973065.1885485/150‑1 (MQ=60)
                                                                             ATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCAT                                                                         <  SRR19973065.412232/150‑1 (MQ=60)
                                                                               CGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAA                                                                       >  SRR19973065.1176581/1‑150 (MQ=60)
                                                                               CGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAA                                                                       <  SRR19973065.171341/150‑1 (MQ=60)
                                                                                 ACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAAT                                                                     <  SRR19973065.770571/150‑1 (MQ=60)
                                                                                 ACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAAT                                                                     <  SRR19973065.761577/150‑1 (MQ=60)
                                                                                 ACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAAT                                                                     <  SRR19973065.761584/150‑1 (MQ=60)
                                                                                 ACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAAT                                                                     >  SRR19973065.2005366/1‑150 (MQ=60)
                                                                                     TAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGATATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTC                                                                 <  SRR19973065.2156349/150‑1 (MQ=60)
                                                                                     TAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTC                                                                 <  SRR19973065.375739/150‑1 (MQ=60)
                                                                                     TAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTC                                                                 <  SRR19973065.375711/150‑1 (MQ=60)
                                                                                        CAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGAAGTAAAATATTTACAGATCGGAGCACGCACCAAAATACAGGATTGCACTATGTTTCATGTaacacataaaaacacgta                                                              >  SRR19973065.1276148/1‑132 (MQ=60)
                                                                                        CAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCGGGGTGTTATTAGTGGAGATTTAAAATATTTAAAGATCGGAGCACCCACCAAAATCCAGGATGGCCGTCTGTTTCATGTCACacaaaaaaccaccta                                                              >  SRR19973065.1276921/1‑135 (MQ=58)
                                                                                         AGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTAC                                                             <  SRR19973065.3095598/150‑1 (MQ=60)
                                                                                             TCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACC                                                         <  SRR19973065.1173053/150‑1 (MQ=60)
                                                                                              CGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCC                                                        >  SRR19973065.1147294/1‑150 (MQ=60)
                                                                                               GTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCA                                                       <  SRR19973065.1954385/150‑1 (MQ=60)
                                                                                               GTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCA                                                       <  SRR19973065.1733942/150‑1 (MQ=60)
                                                                                                TGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAG                                                      >  SRR19973065.2513698/1‑150 (MQ=60)
                                                                                                TGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATACTCGTACAACCCAG                                                      >  SRR19973065.2513323/1‑150 (MQ=60)
                                                                                                  ATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGAT                                                    >  SRR19973065.1176444/1‑150 (MQ=60)
                                                                                                     GGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGC                                                 <  SRR19973065.275877/150‑1 (MQ=60)
                                                                                                      GTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCA                                                <  SRR19973065.3082967/150‑1 (MQ=60)
                                                                                                            TTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCACGTACAACCCACATGGCAACCCAT                                          >  SRR19973065.2074576/1‑150 (MQ=60)
                                                                                                            TTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATACTCGTACAAACCAGATGGCAACCCAT                                          >  SRR19973065.2071242/1‑150 (MQ=60)
                                                                                                             TCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATT                                         <  SRR19973065.433705/150‑1 (MQ=60)
                                                                                                               GTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGTGATTTAAATTATTTAAAGATAGGAGCACGCCCCCAAATACACGATGGCAGTATTTTGCATGTCACACAAAAATACACGTACAAACCACAAGGCAACCCAATAA                                       >  SRR19973065.10398/1‑150 (MQ=33)
                                                                                                               GTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAA                                       <  SRR19973065.1199897/150‑1 (MQ=60)
                                                                                                                   GGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGATATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCAT                                   <  SRR19973065.2398115/150‑1 (MQ=60)
                                                                                                                    GCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATT                                  <  SRR19973065.1479133/150‑1 (MQ=60)
                                                                                                                    GCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATT                                  <  SRR19973065.1478136/150‑1 (MQ=60)
                                                                                                                     CTGATGATGTGGGGATCTGGCCGCTCGTTGTTTTTAGTGGAGATGTTAATTATTTAAAGATCGGATCACGCACCAAAATCCAGGATGGCAGTATGTTGCATGTCACTCAaaaaaacacgtacaaaccccatggcaaccccataaccaatt                                 >  SRR19973065.28800/1‑109 (MQ=60)
                                                                                                                     CTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTG                                 <  SRR19973065.2426267/150‑1 (MQ=60)
                                                                                                                     CTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTG                                 <  SRR19973065.2426236/150‑1 (MQ=60)
                                                                                                                       GATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGC                               <  SRR19973065.3467628/150‑1 (MQ=60)
                                                                                                                              TGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCACATGGCAACCCATTAACCATTGGCGAAGATG                        >  SRR19973065.3483581/1‑150 (MQ=60)
                                                                                                                                GGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTC                      <  SRR19973065.1276357/150‑1 (MQ=60)
                                                                                                                                   ATGTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCTTATAACGCAAGAATATCCAGGATGTCAGTATGTTGCATGTCACTCATAAATCCTCTTACAAGCCAGATGGCTAGCCTTTAACAATTGGCGAAGATGTCACT                   <  SRR19973065.1369713/150‑1 (MQ=60)
                                                                                                                                   ATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACT                   <  SRR19973065.856714/150‑1 (MQ=60)
                                                                                                                                   ATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACT                   <  SRR19973065.1015/150‑1 (MQ=60)
                                                                                                                                   ATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACT                   <  SRR19973065.856721/150‑1 (MQ=60)
                                                                                                                                    TCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTG                  <  SRR19973065.205020/150‑1 (MQ=60)
                                                                                                                                     CTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGT                 <  SRR19973065.2709394/150‑1 (MQ=60)
                                                                                                                                      TGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTT                <  SRR19973065.2970660/150‑1 (MQ=60)
                                                                                                                                      TGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTT                <  SRR19973065.1552225/150‑1 (MQ=60)
                                                                                                                                       GGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTG               <  SRR19973065.3520098/150‑1 (MQ=60)
                                                                                                                                           GCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCA           >  SRR19973065.1698706/1‑150 (MQ=60)
                                                                                                                                            CTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCAC          <  SRR19973065.1033608/150‑1 (MQ=60)
                                                                                                                                               GTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAG       >  SRR19973065.2821776/1‑150 (MQ=60)
                                                                                                                                               GTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAG       >  SRR19973065.2333271/1‑150 (MQ=60)
                                                                                                                                                 TGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGT     >  SRR19973065.1715589/1‑150 (MQ=60)
                                                                                                                                                  GTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTG    >  SRR19973065.2337794/1‑150 (MQ=60)
                                                                                                                                                   TTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGA   <  SRR19973065.405703/150‑1 (MQ=60)
                                                                                                                                                   TTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGA   <  SRR19973065.405570/150‑1 (MQ=60)
                                                                                                                                                    TATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGAT  >  SRR19973065.3468202/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
TTATTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGAT  >  CP009273/3422578‑3422875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑