Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,422,726 G→T V44V (GTG→GTT)  yrdA → bacterial transferase hexapeptide domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,422,7260GT100.0% 527.9 / NA 159V44V (GTG→GTT) yrdAbacterial transferase hexapeptide domain protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (78/81);  total (78/81)

Only 100 of 164 total aligned reads displayed.

TATTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGT  >  CP009273/3422579‑3422872
                                                                                                                                                   |                                                                                                                                                  
tATTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTAt                                                                                                                                                  <  1:3144147/150‑1 (MQ=255)
  ttGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTc                                                                                                                                                >  2:1229013/1‑150 (MQ=255)
  ttGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTc                                                                                                                                                >  2:1229008/1‑150 (MQ=255)
   tGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCg                                                                                                                                               <  1:1449657/150‑1 (MQ=255)
       gAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGga                                                                                                                                           <  2:2726025/150‑1 (MQ=255)
       gAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGga                                                                                                                                           >  2:1770136/1‑150 (MQ=255)
        aGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGgag                                                                                                                                          >  2:3180689/1‑150 (MQ=255)
        aGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGgag                                                                                                                                          >  1:3675107/1‑150 (MQ=255)
          gTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGAt                                                                                                                                        <  1:961681/150‑1 (MQ=255)
           tAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCCCACATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATg                                                                                                                                       <  2:335955/150‑1 (MQ=255)
           tAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATg                                                                                                                                       >  1:1151914/1‑150 (MQ=255)
                aTGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACAt                                                                                                                                  >  2:2443607/1‑150 (MQ=255)
                    cTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATg                                                                                                                              <  1:1473047/150‑1 (MQ=255)
                     tGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGt                                                                                                                             >  1:2890783/1‑150 (MQ=255)
                     tGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGt                                                                                                                             >  1:2891879/1‑150 (MQ=255)
                      gATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTa                                                                                                                            >  2:2219711/1‑150 (MQ=255)
                       aTGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTAc                                                                                                                           >  2:578611/1‑150 (MQ=255)
                         gTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAg                                                                                                                         <  1:1071182/150‑1 (MQ=255)
                           tttACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGAt                                                                                                                       <  2:396527/150‑1 (MQ=255)
                            ttACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATc                                                                                                                      <  2:1376829/150‑1 (MQ=255)
                            ttACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATc                                                                                                                      >  2:191528/1‑150 (MQ=255)
                            ttACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATc                                                                                                                      >  1:2297980/1‑150 (MQ=255)
                             tACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCg                                                                                                                     <  2:2356111/150‑1 (MQ=255)
                             tACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCg                                                                                                                     <  2:425914/150‑1 (MQ=255)
                               cGCCCATACCGCGATCTTTTTCCACAACTCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGa                                                                                                                   <  2:3737694/150‑1 (MQ=255)
                               cGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGa                                                                                                                   <  2:2832125/150‑1 (MQ=255)
                                gCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAg                                                                                                                  >  1:2606585/1‑150 (MQ=255)
                                 cccATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAgc                                                                                                                 >  1:1694142/1‑150 (MQ=255)
                                 cccATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAgc                                                                                                                 <  1:1229008/150‑1 (MQ=255)
                                   cATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAgcac                                                                                                               >  1:625508/1‑150 (MQ=255)
                                      aCCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAgcacgca                                                                                                            <  1:3196181/150‑1 (MQ=255)
                                            aTCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACA‑TATGTACAGATCGGAGCACGCACCAatat                                                                                                     >  2:3705860/1‑150 (MQ=255)
                                             tCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAatat                                                                                                     <  1:2430097/150‑1 (MQ=255)
                                                ttttCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCa                                                                                                  <  1:3282071/150‑1 (MQ=255)
                                                ttttCCACAAATCGGTAAGCGCGTATTGATCGACGATAGAAGTGTCGTGATTGGTGACGTTAGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGTAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCa                                                                                                  <  1:2673310/150‑1 (MQ=255)
                                                 tttCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGATCACGCACCAATATCCAg                                                                                                 >  1:104998/1‑150 (MQ=255)
                                                 tttCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAg                                                                                                 <  1:1311418/150‑1 (MQ=255)
                                                   tCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGa                                                                                               >  1:1244751/1‑150 (MQ=255)
                                                      acaAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATgg                                                                                            <  2:2541196/150‑1 (MQ=255)
                                                      acaAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATgg                                                                                            >  2:1385213/1‑150 (MQ=255)
                                                         aaTCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAg                                                                                         <  2:1646824/150‑1 (MQ=255)
                                                            cGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTAt                                                                                      <  1:1937474/150‑1 (MQ=255)
                                                              gTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGt                                                                                    <  2:1618836/150‑1 (MQ=255)
                                                              gTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGt                                                                                    <  2:491284/150‑1 (MQ=255)
                                                              gTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGt                                                                                    >  2:773621/1‑150 (MQ=255)
                                                               tCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGtt                                                                                   <  1:2015157/150‑1 (MQ=255)
                                                               tCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGtt                                                                                   >  2:3266262/1‑150 (MQ=255)
                                                                 aGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGc                                                                                 >  2:2599794/1‑150 (MQ=255)
                                                                 aGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGc                                                                                 >  2:1041058/1‑150 (MQ=255)
                                                                 aGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGc                                                                                 >  1:2768252/1‑150 (MQ=255)
                                                                  gcgcGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATATACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCa                                                                                <  2:634941/150‑1 (MQ=255)
                                                                     cgTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGt                                                                             <  1:2746382/150‑1 (MQ=255)
                                                                      gTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTc                                                                            <  2:2328952/150‑1 (MQ=255)
                                                                       tAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCa                                                                           >  2:1157318/1‑150 (MQ=255)
                                                                          tGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTc                                                                        >  2:3241364/1‑150 (MQ=255)
                                                                          tGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTc                                                                        >  2:1046671/1‑150 (MQ=255)
                                                                           gATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCa                                                                       >  2:1699570/1‑150 (MQ=255)
                                                                             tCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATa                                                                     <  2:3330048/150‑1 (MQ=255)
                                                                               gacgaTAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATaaa                                                                   >  2:958920/1‑150 (MQ=255)
                                                                                 cgaTAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATc                                                                 >  2:512087/1‑150 (MQ=255)
                                                                                 cgaTAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAACATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATc                                                                 >  2:2781208/1‑150 (MQ=255)
                                                                                   aTAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCt                                                               >  1:305240/1‑150 (MQ=255)
                                                                                     aGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCg                                                             <  2:2762419/150‑1 (MQ=255)
                                                                                     aGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCg                                                             <  1:2202979/150‑1 (MQ=255)
                                                                                       cAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTa                                                           <  2:3322501/150‑1 (MQ=255)
                                                                                        aGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTTTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATACAGGATGGCAGTATTTTGCATGTAACTCAAAAATCCTCGTAc                                                          >  1:3201182/1‑150 (MQ=255)
                                                                                           gtCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAAc                                                       >  2:2257769/1‑150 (MQ=255)
                                                                                           gtCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAAc                                                       <  1:2954234/150‑1 (MQ=255)
                                                                                              gTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCa                                                    <  2:2473936/150‑1 (MQ=255)
                                                                                              gTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCa                                                    >  2:1943801/1‑150 (MQ=255)
                                                                                                 aTTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGAt                                                 >  1:1619620/1‑150 (MQ=255)
                                                                                                 aTTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGAt                                                 >  2:730013/1‑150 (MQ=255)
                                                                                                 aTTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGAt                                                 <  2:3514900/150‑1 (MQ=255)
                                                                                                  ttGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATg                                                >  1:633546/1‑150 (MQ=255)
                                                                                                   tGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATgg                                               <  2:2535454/150‑1 (MQ=255)
                                                                                                     gTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCa                                             >  2:1008181/1‑150 (MQ=255)
                                                                                                      tGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCaa                                            >  2:2622575/1‑150 (MQ=255)
                                                                                                        aCGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAAcc                                          <  2:1373076/150‑1 (MQ=255)
                                                                                                         cGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAAccc                                         >  2:2511052/1‑150 (MQ=255)
                                                                                                          gTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCa                                        >  2:583779/1‑150 (MQ=255)
                                                                                                            tCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCAtt                                      <  2:2924137/150‑1 (MQ=255)
                                                                                                                     tgatgaTGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTgg                             >  1:2835576/1‑150 (MQ=255)
                                                                                                                        tgatgTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGa                          >  1:3108119/1‑150 (MQ=255)
                                                                                                                        tgatgTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGa                          <  1:1601610/150‑1 (MQ=255)
                                                                                                                            gtgGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGAt                      <  1:1273204/150‑1 (MQ=255)
                                                                                                                            gtgGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGAt                      <  1:1273720/150‑1 (MQ=255)
                                                                                                                              ggggATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGt                    <  1:502784/150‑1 (MQ=255)
                                                                                                                               gggATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTc                   <  1:850673/150‑1 (MQ=255)
                                                                                                                                 gATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCAc                 >  1:2458875/1‑150 (MQ=255)
                                                                                                                                   tCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTg               <  2:1694142/150‑1 (MQ=255)
                                                                                                                                    cTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGt              <  1:3099699/150‑1 (MQ=255)
                                                                                                                                     tGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGtt             >  2:3528993/1‑150 (MQ=255)
                                                                                                                                     tGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGtt             >  2:3215144/1‑150 (MQ=255)
                                                                                                                                       gCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTgg           <  1:2443607/150‑1 (MQ=255)
                                                                                                                                            tCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTcaca      >  2:1460549/1‑150 (MQ=255)
                                                                                                                                             cGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACaa     >  1:3138513/1‑150 (MQ=255)
                                                                                                                                              gttgttATTCGTGGAGATGTACATTATGTACATATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCCTTAACCCTTGGCGAAGATGTCACTGTTGGTCACAAg    >  1:763920/1‑150 (MQ=255)
                                                                                                                                               ttGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAgg   <  1:3388792/150‑1 (MQ=255)
                                                                                                                                               ttGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAgg   <  1:2781211/150‑1 (MQ=255)
                                                                                                                                                tgttATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGt  <  1:2339866/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TATTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGT  >  CP009273/3422579‑3422872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTATTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGAT  >  CP009273/3422577‑3422875
                                                                                                                                                     |                                                                                                                                                     
TTTATTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTT                                                                                                                                                       >  SRR19973061.1748948/1‑150 (MQ=60)
  TATTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTAT                                                                                                                                                     <  SRR19973061.3152236/150‑1 (MQ=60)
     TGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCG                                                                                                                                                  <  SRR19973061.1453316/150‑1 (MQ=60)
       CTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTG                                                                                                                                                <  SRR19973061.1275097/150‑1 (MQ=60)
          AGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAG                                                                                                                                             >  SRR19973061.3684500/1‑150 (MQ=60)
            GTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGAT                                                                                                                                           <  SRR19973061.964109/150‑1 (MQ=60)
             TAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATG                                                                                                                                          <  SRR19973061.2548022/150‑1 (MQ=60)
             TAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATG                                                                                                                                          >  SRR19973061.1154839/1‑150 (MQ=60)
                      CTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATG                                                                                                                                 <  SRR19973061.1476747/150‑1 (MQ=60)
                       TGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGT                                                                                                                                >  SRR19973061.262549/1‑150 (MQ=60)
                       TGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGT                                                                                                                                >  SRR19973061.2898197/1‑150 (MQ=60)
                       TGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGT                                                                                                                                >  SRR19973061.2899296/1‑150 (MQ=60)
                         ATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTAC                                                                                                                              >  SRR19973061.3132588/1‑150 (MQ=60)
                           GTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAG                                                                                                                            <  SRR19973061.1073929/150‑1 (MQ=60)
                              TTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATC                                                                                                                         <  SRR19973061.3035363/150‑1 (MQ=60)
                              TTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATC                                                                                                                         >  SRR19973061.2303793/1‑150 (MQ=60)
                                  GCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAG                                                                                                                     >  SRR19973061.2613285/1‑150 (MQ=60)
                                   CCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGC                                                                                                                    <  SRR19973061.1232073/150‑1 (MQ=60)
                                   CCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGC                                                                                                                    <  SRR19973061.1232067/150‑1 (MQ=60)
                                   CCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGC                                                                                                                    >  SRR19973061.1698395/1‑150 (MQ=60)
                                     CATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCAC                                                                                                                  >  SRR19973061.627106/1‑150 (MQ=60)
                                        ACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCA                                                                                                               <  SRR19973061.3204383/150‑1 (MQ=60)
                                        ACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCA                                                                                                               <  SRR19973061.3204419/150‑1 (MQ=60)
                                               TCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATAT                                                                                                        <  SRR19973061.2436301/150‑1 (MQ=60)
                                                  TTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCA                                                                                                     <  SRR19973061.3290527/150‑1 (MQ=60)
                                                  TTTTCCACAAATCGGTAAGCGCGTATTGATCGACGATAGAAGTGTCGTGATTGGTGACGTTAGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGTAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCA                                                                                                     <  SRR19973061.2680187/150‑1 (MQ=60)
                                                   TTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGATCACGCACCAATATCCAG                                                                                                    >  SRR19973061.105349/1‑150 (MQ=60)
                                                   TTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAG                                                                                                    >  SRR19973061.2021317/1‑150 (MQ=60)
                                                   TTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAG                                                                                                    <  SRR19973061.1314704/150‑1 (MQ=60)
                                                     TCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGA                                                                                                  >  SRR19973061.1247835/1‑150 (MQ=60)
                                                           AATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATTTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAG                                                                                            >  SRR19973061.2931652/1‑150 (MQ=60)
                                                              CGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTAT                                                                                         <  SRR19973061.1942293/150‑1 (MQ=60)
                                                                 TCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTT                                                                                      >  SRR19973061.368934/1‑150 (MQ=60)
                                                                 TCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTT                                                                                      <  SRR19973061.2020218/150‑1 (MQ=60)
                                                                   AGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGC                                                                                    >  SRR19973061.2775376/1‑150 (MQ=60)
                                                                    GCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCA                                                                                   <  SRR19973061.3188849/150‑1 (MQ=60)
                                                                       CGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGT                                                                                <  SRR19973061.2753452/150‑1 (MQ=60)
                                                                                CGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAA                                                                       >  SRR19973061.2758486/1‑150 (MQ=60)
                                                                                     ATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCT                                                                  >  SRR19973061.306044/1‑150 (MQ=60)
                                                                                       AGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCG                                                                <  SRR19973061.2208513/150‑1 (MQ=60)
                                                                                          AGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTTTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATACAGGATGGCAGTATTTTGCATGTAACTCAAAAATCCTCGTAC                                                             >  SRR19973061.3209410/1‑150 (MQ=60)
                                                                                          AGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTAC                                                             <  SRR19973061.1002409/150‑1 (MQ=60)
                                                                                             GTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAAC                                                          <  SRR19973061.2961855/150‑1 (MQ=60)
                                                                                                   ATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGAT                                                    >  SRR19973061.1623656/1‑150 (MQ=60)
                                                                                                    TTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATG                                                   >  SRR19973061.635159/1‑150 (MQ=60)
                                                                                                     TGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTAAGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGG                                                  >  SRR19973061.1808713/1‑150 (MQ=60)
                                                                                                       GTGACGTTCGTCTGGATGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCA                                                <  SRR19973061.1378435/150‑1 (MQ=60)
                                                                                                                GTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAA                                       >  SRR19973061.1531616/1‑150 (MQ=60)
                                                                                                                      CTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTG                                 >  SRR19973061.1084499/1‑150 (MQ=60)
                                                                                                                       TGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGG                                >  SRR19973061.2842838/1‑150 (MQ=60)
                                                                                                                          TGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGA                             <  SRR19973061.1605592/150‑1 (MQ=60)
                                                                                                                          TGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGA                             >  SRR19973061.3116129/1‑150 (MQ=60)
                                                                                                                              GTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGAT                         <  SRR19973061.1276858/150‑1 (MQ=60)
                                                                                                                              GTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGAT                         <  SRR19973061.1276342/150‑1 (MQ=60)
                                                                                                                              GTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGAT                         <  SRR19973061.2225282/150‑1 (MQ=60)
                                                                                                                               TGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATG                        <  SRR19973061.1609359/150‑1 (MQ=60)
                                                                                                                                GGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGT                       <  SRR19973061.504108/150‑1 (MQ=60)
                                                                                                                                 GGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTC                      <  SRR19973061.852892/150‑1 (MQ=60)
                                                                                                                                   GATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCAC                    >  SRR19973061.2465137/1‑150 (MQ=60)
                                                                                                                                     TCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTG                  <  SRR19973061.2872539/150‑1 (MQ=60)
                                                                                                                                      CTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGT                 <  SRR19973061.3107982/150‑1 (MQ=60)
                                                                                                                                      CTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGT                 <  SRR19973061.3107684/150‑1 (MQ=60)
                                                                                                                                         GCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGG              <  SRR19973061.2449839/150‑1 (MQ=60)
                                                                                                                                           CGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTC            >  SRR19973061.1292726/1‑150 (MQ=60)
                                                                                                                                           CGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTC            <  SRR19973061.621680/150‑1 (MQ=60)
                                                                                                                                             CTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCAC          >  SRR19973061.2443166/1‑150 (MQ=60)
                                                                                                                                               CGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAA        >  SRR19973061.2365089/1‑150 (MQ=60)
                                                                                                                                               CGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAA        >  SRR19973061.3146589/1‑150 (MQ=60)
                                                                                                                                                GTTGTTATTCGTGGAGATGTACATTATGTACATATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCCTTAACCCTTGGCGAAGATGTCACTGTTGGTCACAAG       >  SRR19973061.765924/1‑150 (MQ=60)
                                                                                                                                                GTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAG       >  SRR19973061.832219/1‑150 (MQ=60)
                                                                                                                                                 TTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGG      <  SRR19973061.3396772/150‑1 (MQ=60)
                                                                                                                                                 TTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGG      <  SRR19973061.3397499/150‑1 (MQ=60)
                                                                                                                                                 TTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGG      <  SRR19973061.2788365/150‑1 (MQ=60)
                                                                                                                                                 TTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAACATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGG      <  SRR19973061.2788362/150‑1 (MQ=60)
                                                                                                                                                  TGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGT     >  SRR19973061.749912/1‑150 (MQ=60)
                                                                                                                                                  TGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGT     <  SRR19973061.2345810/150‑1 (MQ=60)
                                                                                                                                                   GTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTG    >  SRR19973061.2150203/1‑150 (MQ=60)
                                                                                                                                                     TATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGAT  <  SRR19973061.2444261/150‑1 (MQ=60)
                                                                                                                                                     TATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGAT  <  SRR19973061.3735178/150‑1 (MQ=60)
                                                                                                                                                     TATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGAT  >  SRR19973061.3272390/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TTTATTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGAT  >  CP009273/3422577‑3422875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑