Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,422,726 G→T 100% V44V (GTG→GTT)  yrdA → bacterial transferase hexapeptide domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,422,7260GT99.6% 645.1 / ‑5.9 195V44V (GTG→GTT) yrdAbacterial transferase hexapeptide domain protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (89/105);  total (89/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 195 total aligned reads displayed.

TTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCAC  >  CP009273/3422581‑3422867
                                                                                                                                                 |                                                                                                                                             
   cTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCg                                                                                                                                          <  2:3491882/148‑1 (MQ=255)
     gAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTg                                                                                                                                        >  1:3523875/1‑148 (MQ=255)
     gAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTg                                                                                                                                        >  2:2222594/1‑148 (MQ=255)
      aGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTgg                                                                                                                                       <  2:1126844/148‑1 (MQ=255)
        gTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGgag                                                                                                                                     <  2:568646/148‑1 (MQ=255)
        gTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGgag                                                                                                                                     >  2:1407341/1‑148 (MQ=255)
        gTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGgag                                                                                                                                     <  1:2404687/148‑1 (MQ=255)
          aaGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGAt                                                                                                                                   >  1:2956426/1‑148 (MQ=255)
          aaGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGAt                                                                                                                                   >  1:2316098/1‑148 (MQ=255)
           aGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATg                                                                                                                                  <  1:3057119/148‑1 (MQ=255)
           aGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATg                                                                                                                                  <  1:2659203/148‑1 (MQ=255)
            gTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGt                                                                                                                                 <  1:2466227/148‑1 (MQ=255)
            gTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGt                                                                                                                                 <  1:1381041/148‑1 (MQ=255)
              aTGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATGTAc                                                                                                                               >  2:1518785/1‑148 (MQ=255)
               tGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACa                                                                                                                              >  2:3559296/1‑148 (MQ=255)
                 tCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACAtt                                                                                                                            <  2:1279975/148‑1 (MQ=255)
                   tGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTAt                                                                                                                          >  2:3062064/1‑148 (MQ=255)
                    gATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATg                                                                                                                         >  2:3654136/1‑148 (MQ=255)
                    gATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATg                                                                                                                         <  1:1408312/148‑1 (MQ=255)
                     aTGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGt                                                                                                                        <  2:1931953/148‑1 (MQ=255)
                      tGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTa                                                                                                                       >  1:3470439/1‑148 (MQ=255)
                      tGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTa                                                                                                                       >  1:3192112/1‑148 (MQ=255)
                      tGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTa                                                                                                                       <  2:2902071/148‑1 (MQ=255)
                       gTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTAc                                                                                                                      >  2:3147166/1‑148 (MQ=255)
                       gTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTAc                                                                                                                      <  1:1297558/148‑1 (MQ=255)
                        ttttACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGTGATGTACATTATGTACa                                                                                                                     >  2:337849/1‑148 (MQ=255)
                        ttttACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACa                                                                                                                     >  2:338103/1‑148 (MQ=255)
                              gCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCgg                                                                                                               <  2:349605/148‑1 (MQ=255)
                                ccATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAg                                                                                                             <  1:1489507/148‑1 (MQ=255)
                                 cATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCGCGTTGGTTTTAGTGGGGATGTACATTATGTACAGATCGgcgc                                                                                                            >  1:345831/1‑148 (MQ=255)
                                  aTACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAgca                                                                                                           <  2:3290828/148‑1 (MQ=255)
                                    aCCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAgcacg                                                                                                         >  1:766903/1‑148 (MQ=255)
                                       gcgATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAgcacgcac                                                                                                      <  2:2410649/148‑1 (MQ=255)
                                        cgATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCAcc                                                                                                     >  2:1683971/1‑148 (MQ=255)
                                          aTCTTTTTCCACAAATCGGTCAGCGCGTAATGATAGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCCTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGcaacaa                                                                                                   >  2:2635871/1‑148 (MQ=255)
                                           tCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAat                                                                                                  >  1:1233213/1‑148 (MQ=255)
                                           tCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAat                                                                                                  <  1:1914240/148‑1 (MQ=255)
                                              ttttCCACAAATCTGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATc                                                                                               <  2:3763418/148‑1 (MQ=255)
                                              ttttCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATc                                                                                               <  2:617847/148‑1 (MQ=255)
                                              ttttCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATc                                                                                               <  2:3095404/148‑1 (MQ=255)
                                                  ccACAAATCGGTCAGCGCGTAATGATCGTCGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAgg                                                                                           <  2:2572707/148‑1 (MQ=255)
                                                   cacaAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGa                                                                                          <  2:2810689/148‑1 (MQ=255)
                                                       aaTCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTTGTATTAGTGGAGATTTAAAATATGTAAAGATCCGAGCACCCACCAATAAACACGATGGc                                                                                      >  1:3355326/1‑148 (MQ=255)
                                                       aaTCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGc                                                                                      <  1:2453796/148‑1 (MQ=255)
                                                        aTCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCa                                                                                     >  2:2885407/1‑148 (MQ=255)
                                                          cGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTGGTGGAGATGTTCATTATGTACAGATCGGAGCACGCCCCAATATACAGGATGGCAGt                                                                                   >  1:524654/1‑148 (MQ=255)
                                                           ggTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTa                                                                                  <  1:1527906/148‑1 (MQ=255)
                                                                gcgcGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTg                                                                             >  2:3172746/1‑148 (MQ=255)
                                                                gcgcGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTg                                                                             >  2:2633360/1‑148 (MQ=255)
                                                                 cgcgTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTAAAGATAGGAGCACGCACCAATATACAGGATGGCAGTATGTTGc                                                                            >  1:3727098/1‑148 (MQ=255)
                                                                   cgTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCCGTATGTTGCAt                                                                          >  2:370795/1‑148 (MQ=255)
                                                                      aaTGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTTTTTTTAGTGGTGAATTTCATTAATTAAAGATACGAGCACGCCCCAATAAACAAGAATGGACTATGTTGCAAGTc                                                                       >  1:371851/1‑148 (MQ=255)
                                                                      aaTGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTc                                                                       >  2:228532/1‑148 (MQ=255)
                                                                      aaTGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTc                                                                       >  2:570972/1‑148 (MQ=255)
                                                                       aTGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCa                                                                      <  1:451214/148‑1 (MQ=255)
                                                                       aTGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCa                                                                      <  2:2819918/148‑1 (MQ=255)
                                                                        tGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCAc                                                                     >  2:1865571/1‑148 (MQ=255)
                                                                        tGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGGGGAGATTTTCATTATGTACAGATCGGCGCCCGCCCCCATATCCAGGCTGGCAGTATGTTGCATGTGAc                                                                     >  1:1735982/1‑148 (MQ=255)
                                                                         gATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTGGTGGAGATGTACATTATTTAAAGATCGGAGCACGCACCAAAATCAACGATTGCAGGATGTTTCATGTAAca                                                                    >  1:385618/1‑147 (MQ=255)
                                                                              acgaTAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATaa                                                               <  1:1667106/148‑1 (MQ=255)
                                                                                 aTAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATc                                                            <  1:291666/148‑1 (MQ=255)
                                                                                  tAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATcc                                                           >  1:1888491/1‑148 (MQ=255)
                                                                                   aGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCt                                                          <  2:1307658/148‑1 (MQ=255)
                                                                                          tCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACaa                                                   <  2:208364/148‑1 (MQ=255)
                                                                                          tCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACaa                                                   >  2:1268959/1‑148 (MQ=255)
                                                                                           cGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTAAATTATGTACAGATCGGAGCACGCACCAAAATCCAGGATGGCAGTATGTTGCATGTCACTCATAAAAACACGTACAAc                                                  >  1:3267721/1‑148 (MQ=255)
                                                                                           cGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTAATCGTGGAGATGTACATTAATTAAAGATCCGAGCACGCACCAAAATCCAAGAATGCCGTATGTTTAATTTCAATCATAAATACACGTACaaa                                                  >  1:727393/1‑147 (MQ=255)
                                                                                             tGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAAccc                                                <  2:2316098/148‑1 (MQ=255)
                                                                                             tGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAAccc                                                >  2:3625132/1‑148 (MQ=255)
                                                                                             tGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGTTGTACAATATGTACAGATCGGAGCACGCACCAAAATCCAGGATGGCAGTATGTTGCATGTCACTAAAAAATACTCGTAAAAccc                                                >  1:1450655/1‑148 (MQ=255)
                                                                                              gATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGGTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCCCGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCa                                               >  1:2072952/1‑148 (MQ=255)
                                                                                               aTTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAg                                              <  1:2083262/148‑1 (MQ=255)
                                                                                                ttGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGa                                             <  1:1685606/148‑1 (MQ=255)
                                                                                                ttGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGa                                             <  1:3820499/148‑1 (MQ=255)
                                                                                                ttGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGa                                             <  1:2213326/148‑1 (MQ=255)
                                                                                                   gTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAAACCTCGTACAACCCAGATgg                                          >  1:924849/1‑148 (MQ=255)
                                                                                                    tGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAAAATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCACATGGc                                         >  1:3137067/1‑148 (MQ=255)
                                                                                                    tGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATGTACATTATTTACAGATCGGAGCACCCACCAATAACCAGGATGGCAGTATTTTGCATGTCACTCATAAATACTCGTACAACCCAGATGGc                                         >  1:224111/1‑148 (MQ=255)
                                                                                                       cGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATTTTAAGATAGGAGCACGCACCAATATACAGGATGGCAGTATTTTGGATGTCAATCATAAATACTCGTACAACCCACATTGCAAc                                      >  1:1332907/1‑148 (MQ=255)
                                                                                                             tCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTa                                <  2:3700762/148‑1 (MQ=255)
                                                                                                              cTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTaa                               <  2:3815374/148‑1 (MQ=255)
                                                                                                              cTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTaa                               >  2:2654236/1‑148 (MQ=255)
                                                                                                              cTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTAATCGTGGAGATGTACAATATTTACATATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTAACTCATAAAACCTCGTACAACCCAGATTGCAACCCATTaa                               >  2:1568993/1‑148 (MQ=255)
                                                                                                                 gCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTAAAACCCAGATGGCAACCCATTAAACa                            >  2:3522995/1‑148 (MQ=255)
                                                                                                                 gCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATTTACATTATGTACAGATCGGAGCACGCACCAAAAACCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCa                            >  1:896235/1‑148 (MQ=255)
                                                                                                                  cTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCAt                           <  1:2083668/148‑1 (MQ=255)
                                                                                                                    gatgatGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTg                         >  2:2356478/1‑148 (MQ=255)
                                                                                                                       gatgTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCg                      >  2:465552/1‑148 (MQ=255)
                                                                                                                          gtgGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAg                   <  1:56056/148‑1 (MQ=255)
                                                                                                                          gtgGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAAAATACAGGATGGCAGTATGTTGCATGTCACACATAAATACTCGTACAACCCAGATGGCAACCCAATAAACATTGGCGTAg                   >  1:2736037/1‑148 (MQ=255)
                                                                                                                           tgGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAAAATACAGGATGGCAGTATTTTGCAGGTCACTCATAAAACCTCGTACAACCCAGATTGCAACCCATTAACCCTTGGCGAAGa                  >  1:1134674/1‑148 (MQ=255)
                                                                                                                              ggATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGt               <  1:208886/148‑1 (MQ=255)
                                                                                                                                aTCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCa             <  2:2956426/148‑1 (MQ=255)
                                                                                                                                 tCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCAc            >  2:1838725/1‑148 (MQ=255)
                                                                                                                                    ggCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGt         <  1:3616452/148‑1 (MQ=255)
                                                                                                                                      ccGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTg       <  2:224111/148‑1 (MQ=255)
                                                                                                                                        gCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGt     <  1:2502439/148‑1 (MQ=255)
                                                                                                                                           cGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTcac  <  2:1894610/148‑1 (MQ=255)
                                                                                                                                           cGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTcac  >  1:416927/1‑148 (MQ=255)
                                                                                                                                           cGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTcac  <  2:727393/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
TTGCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCAC  >  CP009273/3422581‑3422867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGAT  >  CP009273/3422583‑3422875
                                                                                                                                               |                                                                                                                                                     
GCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGT                                                                                                                                                 <  SRR19973069.739885/150‑1 (MQ=60)
 CTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGGTGTTATTAGTG                                                                                                                                                >  SRR19973069.3546657/1‑150 (MQ=60)
     GGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGA                                                                                                                                            >  SRR19973069.2043058/1‑150 (MQ=60)
      GTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGAT                                                                                                                                           <  SRR19973069.2420956/150‑1 (MQ=60)
      GTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGAT                                                                                                                                           >  SRR19973069.2976044/1‑150 (MQ=60)
       TAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATG                                                                                                                                          >  SRR19973069.1285381/1‑150 (MQ=60)
         AGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTA                                                                                                                                        <  SRR19973069.2677027/150‑1 (MQ=60)
         AGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTA                                                                                                                                        <  SRR19973069.3077347/150‑1 (MQ=60)
          GTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTAC                                                                                                                                       <  SRR19973069.2482761/150‑1 (MQ=60)
          GTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTAC                                                                                                                                       <  SRR19973069.2482782/150‑1 (MQ=60)
          GTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTAC                                                                                                                                       <  SRR19973069.3137080/150‑1 (MQ=60)
          GTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTAC                                                                                                                                       <  SRR19973069.1390675/150‑1 (MQ=60)
              GTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTA                                                                                                                                   >  SRR19973069.210022/1‑150 (MQ=60)
                CTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATG                                                                                                                                 <  SRR19973069.2316884/150‑1 (MQ=60)
                 TGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGT                                                                                                                                <  SRR19973069.788666/150‑1 (MQ=60)
                  GATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTA                                                                                                                               >  SRR19973069.3212836/1‑150 (MQ=60)
                  GATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTA                                                                                                                               >  SRR19973069.3492884/1‑150 (MQ=60)
                  GATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTA                                                                                                                               <  SRR19973069.353035/150‑1 (MQ=60)
                  GATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTA                                                                                                                               >  SRR19973069.1745154/1‑150 (MQ=60)
                  GATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTA                                                                                                                               <  SRR19973069.538442/150‑1 (MQ=60)
                  GATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTA                                                                                                                               <  SRR19973069.1418139/150‑1 (MQ=60)
                   ATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTAC                                                                                                                              <  SRR19973069.3247783/150‑1 (MQ=60)
                   ATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATGTACATTATGTAC                                                                                                                              >  SRR19973069.210433/1‑150 (MQ=60)
                     GTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAG                                                                                                                            <  SRR19973069.1306312/150‑1 (MQ=60)
                     GTTTTACGCCCATACCGCGATCTTTCTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAG                                                                                                                            <  SRR19973069.1306523/150‑1 (MQ=60)
                         TACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCG                                                                                                                        <  SRR19973069.1386520/150‑1 (MQ=60)
                             CCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCGCGTTGGTTTTAGTGGGGATGTACATTATGTACAGATCGGCGC                                                                                                                    >  SRR19973069.348289/1‑150 (MQ=60)
                              CCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCA                                                                                                                   <  SRR19973069.1499851/150‑1 (MQ=60)
                               CATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATTTACATTATTTAAAGATCGGAGCAC                                                                                                                  >  SRR19973069.40217/1‑150 (MQ=60)
                                ATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATGTACATTATTTACAGATCGGAGCACG                                                                                                                 >  SRR19973069.772316/1‑150 (MQ=60)
                                    CGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTAAATTATGTACAGATCGGAGCACCCACC                                                                                                             >  SRR19973069.3019497/1‑150 (MQ=60)
                                       GATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAAT                                                                                                          >  SRR19973069.929238/1‑150 (MQ=60)
                                       GATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAAT                                                                                                          >  SRR19973069.1241619/1‑150 (MQ=60)
                                        ATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTGGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATA                                                                                                         >  SRR19973069.2090674/1‑150 (MQ=60)
                                         TCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATAT                                                                                                        <  SRR19973069.1927432/150‑1 (MQ=60)
                                                 CACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATG                                                                                                <  SRR19973069.1539711/150‑1 (MQ=60)
                                                  ACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGG                                                                                               <  SRR19973069.251003/150‑1 (MQ=60)
                                                     AATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAG                                                                                            <  SRR19973069.3680693/150‑1 (MQ=60)
                                                     AATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAG                                                                                            <  SRR19973069.2470294/150‑1 (MQ=60)
                                                     AATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAG                                                                                            <  SRR19973069.2470297/150‑1 (MQ=60)
                                                      ATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTGGTGGAGATGTTCATTATGTACAGATCGGAGCACGCCCCAATATACAGGATGGCAGT                                                                                           >  SRR19973069.528370/1‑150 (MQ=60)
                                                         GGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATG                                                                                        <  SRR19973069.3447960/150‑1 (MQ=60)
                                                         GGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATG                                                                                        <  SRR19973069.1538415/150‑1 (MQ=60)
                                                           TCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTT                                                                                      <  SRR19973069.1419566/150‑1 (MQ=60)
                                                             AGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTAAAGATAGGAGCACGCACCAATATACAGGATGGCAGTATGTTGC                                                                                    >  SRR19973069.3751413/1‑150 (MQ=60)
                                                                  GTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGGTGTTATTCGTGGAGATGTACATTATGTAAATATAGGAGCACGCACCAATAAACAGGATGGCCGGATGTTGCATGTG                                                                               >  SRR19973069.50533/1‑150 (MQ=60)
                                                                    AATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGGGGAGATTTTCATTATGTACAGATCGGCGCCCGCCCCCATATCCAGGCTGGCAGTATGTTGCATGTGAC                                                                             >  SRR19973069.1748152/1‑150 (MQ=60)
                                                                     ATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTGGTGGAGATGTACATTATTTAAAGATCGGAGCACGCACCAAaatcaacgattgcaggatgtttcatgtaaca                                                                            >  SRR19973069.388446/1‑119 (MQ=60)
                                                                     ATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACT                                                                            <  SRR19973069.454514/150‑1 (MQ=60)
                                                                      TGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGTTGTACATTATGTACAGATAGGAGCACGCACCAAAATCCAGGATGGCAGTATGTTGCATGTCACTC                                                                           >  SRR19973069.151141/1‑150 (MQ=60)
                                                                          CGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAA                                                                       >  SRR19973069.3181379/1‑150 (MQ=60)
                                                                            ACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAAT                                                                     <  SRR19973069.1679828/150‑1 (MQ=60)
                                                                            ACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAAT                                                                     <  SRR19973069.1678668/150‑1 (MQ=60)
                                                                              GATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCC                                                                   <  SRR19973069.1498292/150‑1 (MQ=60)
                                                                              GATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCC                                                                   >  SRR19973069.1901598/1‑150 (MQ=60)
                                                                              GATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCC                                                                   <  SRR19973069.2237626/150‑1 (MQ=60)
                                                                              GATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCC                                                                   <  SRR19973069.2858149/150‑1 (MQ=60)
                                                                               ATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCT                                                                  <  SRR19973069.293672/150‑1 (MQ=60)
                                                                                TAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTC                                                                 <  SRR19973069.3492617/150‑1 (MQ=60)
                                                                                TAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCGCGGTGTTATTAGTGGAGATGTAaaatattttcacatcggagcacccaccaaaaaacacgcttgccgtatgtttcaagtaacacaaaaaaacac                                                                 >  SRR19973069.1329939/1‑79 (MQ=60)
                                                                                       GTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGCGATGTTCAATATTTAAAGATAGGAGCACGCACCAATATACAGGATGGCAGTATTTTTCATGTGAATCATAAATACTCGTACAAC                                                          >  SRR19973069.730686/1‑150 (MQ=60)
                                                                                       GTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAAC                                                          >  SRR19973069.1907733/1‑150 (MQ=60)
                                                                                       GTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTAAATTATGTACAGATCGGAGCACGCACCAAAATCCAGGATGGCAGTATGTTGCATGTCACTCATAAAAACACGTACAAC                                                          >  SRR19973069.3289126/1‑150 (MQ=60)
                                                                                       GTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTAATCGTGGAGATGTACATTAATTAAAGATCCGAGCACGCACCAAAATCCAagaatgccgtatgtttaatttcaatcataaatacacgtacaaa                                                          >  SRR19973069.732533/1‑107 (MQ=60)
                                                                                         CGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGTTGTACAATATGTACAGATCGGAGCACGCACCAAAATCCAGGATGGCAGTATGTTGCATGTCACTAAAAAATACTCGTAAAACCC                                                        >  SRR19973069.1460784/1‑150 (MQ=60)
                                                                                          GTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGGTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCCCGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCA                                                       >  SRR19973069.2087310/1‑150 (MQ=60)
                                                                                             ATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGAT                                                    <  SRR19973069.2097675/150‑1 (MQ=60)
                                                                                              TTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATG                                                   <  SRR19973069.1697372/150‑1 (MQ=60)
                                                                                              TTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATG                                                   <  SRR19973069.3844650/150‑1 (MQ=60)
                                                                                              TTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATG                                                   <  SRR19973069.2227163/150‑1 (MQ=60)
                                                                                              TTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATG                                                   <  SRR19973069.2228322/150‑1 (MQ=60)
                                                                                              TTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATG                                                   <  SRR19973069.3845216/150‑1 (MQ=60)
                                                                                               TGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAAACCTCGTACAACCCAGATGG                                                  >  SRR19973069.931145/1‑150 (MQ=60)
                                                                                                GGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAAAATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCACATGGC                                                 >  SRR19973069.3157621/1‑150 (MQ=60)
                                                                                                GGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATGTACATTATTTACAGATCGGAGCACCCACCAATAACCAGGATGGCAGTATTTTGCATGTCACTCATAAATACTCGTACAACCCAGATGGC                                                 >  SRR19973069.225841/1‑150 (MQ=60)
                                                                                                   GACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATTTTAAGATAGGAGCACGCACCAATATACAGGATGGCAGTATTTTGGATGTCAATCATAAATACTCGTACAACCCACATTGCAAC                                              >  SRR19973069.1342093/1‑150 (MQ=60)
                                                                                                             TGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTAGTGGAGATTTACATTATGTACAGATCGGAGCACGCACCAAAAACCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCACATGGCAACCCAATAACCA                                    >  SRR19973069.902411/1‑150 (MQ=60)
                                                                                                                CTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTG                                 <  SRR19973069.2098088/150‑1 (MQ=60)
                                                                                                                CTGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTAAAGATCGGAGCACCCCCCAATATCCACGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTG                                 >  SRR19973069.3749944/1‑150 (MQ=60)
                                                                                                                 TGATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGG                                <  SRR19973069.2676406/150‑1 (MQ=60)
                                                                                                                  GATGATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGC                               <  SRR19973069.1835477/150‑1 (MQ=60)
                                                                                                                      ATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATACAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATAGGCGAAG                           >  SRR19973069.2754020/1‑150 (MQ=60)
                                                                                                                      ATGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAAAATACAGGATGGCAGTATGTTGCATGTCACACATAAATACTCGTACAACCCAGATGGCAACCCAATAAACATTGGCGTAG                           >  SRR19973069.2754342/1‑150 (MQ=60)
                                                                                                                       TGTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAAAATACAGGATGGCAGTATTTTGCAGGTCACTCATAAAACCTCGTACAACCCAGATTGCAACCCATTAACCCTTGGCGAAGA                          >  SRR19973069.1142595/1‑150 (MQ=60)
                                                                                                                        GTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGAT                         <  SRR19973069.56741/150‑1 (MQ=60)
                                                                                                                        GTGGGGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGAT                         <  SRR19973069.2955790/150‑1 (MQ=60)
                                                                                                                            GGATCTGGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCA                     <  SRR19973069.210544/150‑1 (MQ=60)
                                                                                                                                  GGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTG               <  SRR19973069.3640070/150‑1 (MQ=60)
                                                                                                                                  GGCCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTG               <  SRR19973069.3639932/150‑1 (MQ=60)
                                                                                                                                    CCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGT             <  SRR19973069.3334791/150‑1 (MQ=60)
                                                                                                                                    CCGCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGT             <  SRR19973069.1417162/150‑1 (MQ=60)
                                                                                                                                      GCTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCA           <  SRR19973069.2519145/150‑1 (MQ=60)
                                                                                                                                       CTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCAC          >  SRR19973069.419987/1‑150 (MQ=60)
                                                                                                                                       CTCGTTGTTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCAC          >  SRR19973069.419984/1‑150 (MQ=60)
                                                                                                                                              TTATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGA   >  SRR19973069.1386412/1‑150 (MQ=60)
                                                                                                                                               TATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGAT  <  SRR19973069.101821/150‑1 (MQ=60)
                                                                                                                                               |                                                                                                                                                     
GCTGAGGTAAGTATGTCTGATGTTTTACGCCCATACCGCGATCTTTTTCCACAAATCGGTCAGCGCGTAATGATCGACGATAGCAGTGTCGTGATTGGTGACGTTCGTCTGGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGAT  >  CP009273/3422583‑3422875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑