Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,172,507 C→A 92.6% I445I (ATC→ATA)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,172,5070CA92.6% 523.0 / 34.1 190I445I (ATC→ATA) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (7/7);  new base A (96/80);  total (103/87)
Fisher's exact test for biased strand distribution p-value = 7.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01

Only 100 of 194 total aligned reads displayed.

TCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATC  >  CP009273/4172364‑4172651
                                                                                                                                               |                                                                                                                                                
       ttGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCgg                                                                                                                                       >  1:543291/1‑148 (MQ=255)
            cGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAAcc                                                                                                                                  <  1:1560344/148‑1 (MQ=255)
              tATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACcgt                                                                                                                                <  1:2828342/148‑1 (MQ=255)
              tATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACcgt                                                                                                                                >  1:1497885/1‑148 (MQ=255)
               aTGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACcgtc                                                                                                                               >  2:2019638/1‑148 (MQ=255)
                 gAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACcgtcgt                                                                                                                             >  2:3327446/1‑148 (MQ=255)
                    gTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtc                                                                                                                          >  1:2747824/1‑148 (MQ=255)
                    gTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtc                                                                                                                          >  1:2741218/1‑148 (MQ=255)
                     ttCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtcc                                                                                                                         >  1:366644/1‑148 (MQ=255)
                         aCCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtccgttc                                                                                                                     >  1:1828330/1‑148 (MQ=255)
                          ccGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtccgttcc                                                                                                                    >  1:1329626/1‑148 (MQ=255)
                           cGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtccgttccg                                                                                                                   <  2:2170048/148‑1 (MQ=255)
                           cGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtccgttccg                                                                                                                   <  2:2239335/148‑1 (MQ=255)
                            gTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtccgttccgt                                                                                                                  <  1:2695099/148‑1 (MQ=255)
                              tctcTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTg                                                                                                                >  1:2917594/1‑148 (MQ=255)
                              tctcTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTg                                                                                                                >  1:1600521/1‑148 (MQ=255)
                              tctcTGCTGCGCGAAGAAATCGAACGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTAATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTg                                                                                                                >  1:2917647/1‑148 (MQ=255)
                                   gctgcGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGaa                                                                                                           <  1:2595554/148‑1 (MQ=255)
                                   gctgcGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGaa                                                                                                           >  1:49463/1‑148 (MQ=255)
                                   gctgcGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGaa                                                                                                           <  2:2323649/148‑1 (MQ=255)
                                    ctgcGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGaaa                                                                                                          <  1:1967049/148‑1 (MQ=255)
                                      gcgcGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATg                                                                                                        <  2:429315/148‑1 (MQ=255)
                                           aagaaATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGa                                                                                                   <  1:1918368/148‑1 (MQ=255)
                                           aagaaATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGa                                                                                                   >  1:3155751/1‑148 (MQ=255)
                                              aaaTCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGaaaa                                                                                                <  2:184648/148‑1 (MQ=255)
                                                aTCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAAcc                                                                                              >  2:3512420/1‑148 (MQ=255)
                                                aTCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAAcc                                                                                              >  2:697844/1‑148 (MQ=255)
                                                  cGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCTTTCCGTTGGCGAAATGGCGGAAAACCAg                                                                                            <  2:2568393/148‑1 (MQ=255)
                                                  cGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAg                                                                                            <  1:3199750/148‑1 (MQ=255)
                                                    aaGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGtt                                                                                          >  1:2623068/1‑148 (MQ=255)
                                                    aaGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGtt                                                                                          <  2:1142422/148‑1 (MQ=255)
                                                          ccGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGt                                                                                    <  1:1226287/148‑1 (MQ=255)
                                                            ggTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTg                                                                                  <  1:789480/148‑1 (MQ=255)
                                                            ggTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTg                                                                                  <  1:789566/148‑1 (MQ=255)
                                                             gTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTgg                                                                                 >  1:2390723/1‑148 (MQ=255)
                                                              tATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGc                                                                                >  2:3090748/1‑148 (MQ=255)
                                                               aTCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGcc                                                                               <  2:1804433/148‑1 (MQ=255)
                                                                 ccTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTg                                                                             >  2:2505589/1‑148 (MQ=255)
                                                                 ccTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTg                                                                             >  2:1145341/1‑148 (MQ=255)
                                                                  cTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTgg                                                                            <  1:2353406/148‑1 (MQ=255)
                                                                     aGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTAc                                                                         >  1:1552245/1‑148 (MQ=255)
                                                                      gCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACg                                                                        >  2:2178286/1‑148 (MQ=255)
                                                                         aaGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACgtgt                                                                     >  1:2336315/1‑148 (MQ=255)
                                                                         aaGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACgtgt                                                                     <  1:3327446/148‑1 (MQ=255)
                                                                         aaGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACgtgt                                                                     <  1:1906484/148‑1 (MQ=255)
                                                                          aGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTa                                                                    <  2:1233697/148‑1 (MQ=255)
                                                                               acATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCg                                                               >  2:1142720/1‑148 (MQ=255)
                                                                               acATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCg                                                               >  2:2849214/1‑148 (MQ=255)
                                                                                catcatTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGt                                                              >  2:178690/1‑148 (MQ=255)
                                                                                   catTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCg                                                           >  1:1322916/1‑148 (MQ=255)
                                                                                   catTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCg                                                           <  2:1801826/148‑1 (MQ=255)
                                                                                      tGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTg                                                        >  1:1641263/1‑148 (MQ=255)
                                                                                         tGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGaaa                                                     >  2:2392494/1‑148 (MQ=255)
                                                                                          gTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAag                                                    <  1:429763/148‑1 (MQ=255)
                                                                                          gTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAag                                                    >  1:1153095/1‑148 (MQ=255)
                                                                                             aTGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGc                                                 >  1:1666833/1‑148 (MQ=255)
                                                                                               gAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgt                                               <  2:840946/148‑1 (MQ=255)
                                                                                                 aaaaGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtct                                             <  2:1406821/148‑1 (MQ=255)
                                                                                                   aaGCTTATCTATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTACGTTGGCGAAATGGCGGAAATCCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtctgt                                           <  2:509948/148‑1 (MQ=255)
                                                                                                    aGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtctgtc                                          >  2:2301676/1‑148 (MQ=255)
                                                                                                     gCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtctgtct                                         <  2:3340636/148‑1 (MQ=255)
                                                                                                     gCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtctgtct                                         <  2:2970804/148‑1 (MQ=255)
                                                                                                      cTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGtctc                                        >  1:3299165/1‑148 (MQ=255)
                                                                                                      cTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGtctc                                        >  1:2126384/1‑148 (MQ=255)
                                                                                                       tCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTctct                                       <  1:697844/148‑1 (MQ=255)
                                                                                                        cATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTg                                      >  2:1463188/1‑148 (MQ=255)
                                                                                                        cATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTg                                      >  2:590111/1‑148 (MQ=255)
                                                                                                         aTCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTgg                                     >  2:2724356/1‑148 (MQ=255)
                                                                                                         aTCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTgg                                     >  1:2222572/1‑148 (MQ=255)
                                                                                                          tCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTggg                                    <  2:2747824/148‑1 (MQ=255)
                                                                                                           cGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGc                                   >  1:3392044/1‑148 (MQ=255)
                                                                                                               atCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATc                               >  2:1051566/1‑148 (MQ=255)
                                                                                                                    tAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGAt                          >  1:2550184/1‑148 (MQ=255)
                                                                                                                    tAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGAt                          >  1:2489939/1‑148 (MQ=255)
                                                                                                                    tAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGAt                          >  1:2487919/1‑148 (MQ=255)
                                                                                                                      aCGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATAc                        >  2:1216712/1‑148 (MQ=255)
                                                                                                                      aCGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATAc                        >  2:2657096/1‑148 (MQ=255)
                                                                                                                       cGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATAcc                       >  2:3135624/1‑148 (MQ=255)
                                                                                                                       cGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATAcc                       >  2:946268/1‑148 (MQ=255)
                                                                                                                          tAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTg                    <  2:3246703/148‑1 (MQ=255)
                                                                                                                           aaaGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGa                   <  2:2741218/148‑1 (MQ=255)
                                                                                                                           aaaGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGa                   >  1:1004040/1‑148 (MQ=255)
                                                                                                                           aaaGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGa                   <  1:1989968/148‑1 (MQ=255)
                                                                                                                           aaaGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGa                   >  1:2985308/1‑148 (MQ=255)
                                                                                                                           aaaGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGa                   <  2:2192503/148‑1 (MQ=255)
                                                                                                                              ggCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGc                <  1:1039407/148‑1 (MQ=255)
                                                                                                                               gCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGcc               >  2:1109164/1‑148 (MQ=255)
                                                                                                                                   aGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAg           <  2:1555489/148‑1 (MQ=255)
                                                                                                                                    gTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAgg          <  1:946268/148‑1 (MQ=255)
                                                                                                                                    gTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAgg          <  1:2725465/148‑1 (MQ=255)
                                                                                                                                     tCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAggc         >  2:3249152/1‑147 (MQ=255)
                                                                                                                                      cGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGat        <  2:573918/148‑1 (MQ=255)
                                                                                                                                       gatgatATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGata       <  1:3020008/148‑1 (MQ=255)
                                                                                                                                         tgatATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATg     >  1:3308781/1‑148 (MQ=255)
                                                                                                                                         tgatATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATg     >  1:2162295/1‑148 (MQ=255)
                                                                                                                                          gatATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGa    >  1:3515399/1‑148 (MQ=255)
                                                                                                                                            tataGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGCTc  >  2:563434/1‑148 (MQ=255)
                                                                                                                                            tataGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATc  >  2:3016127/1‑148 (MQ=255)
                                                                                                                                            tataGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATc  <  1:3508396/148‑1 (MQ=255)
                                                                                                                                            tataGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATc  >  2:1378381/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                
TCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATC  >  CP009273/4172364‑4172651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAAC  >  CP009273/4172359‑4172654
                                                                                                                                                    |                                                                                                                                                   
ACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAG                                                                                                                                                    >  SRR19973067.1086959/1‑150 (MQ=60)
ACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAG                                                                                                                                                    >  SRR19973067.1087031/1‑150 (MQ=60)
  TTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGAC                                                                                                                                                  <  SRR19973067.1969939/150‑1 (MQ=60)
   TGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACC                                                                                                                                                 >  SRR19973067.3381841/1‑150 (MQ=60)
    GTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCA                                                                                                                                                >  SRR19973067.2338918/1‑150 (MQ=60)
         CGGTTTGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCG                                                                                                                                           <  SRR19973067.477535/150‑1 (MQ=60)
          GGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGG                                                                                                                                          >  SRR19973067.546896/1‑150 (MQ=60)
           GTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGC                                                                                                                                         >  SRR19973067.1928356/1‑150 (MQ=60)
                 CGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGT                                                                                                                                   <  SRR19973067.1570997/150‑1 (MQ=60)
                 CGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGT                                                                                                                                   >  SRR19973067.1507756/1‑150 (MQ=60)
                 CGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGT                                                                                                                                   >  SRR19973067.1507757/1‑150 (MQ=60)
                   TATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCG                                                                                                                                 <  SRR19973067.2846529/150‑1 (MQ=60)
                     TGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTA                                                                                                                               <  SRR19973067.2752821/150‑1 (MQ=60)
                       ANGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATC                                                                                                                             >  SRR19973067.2765582/1‑150 (MQ=60)
                       AAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATC                                                                                                                             >  SRR19973067.2758955/1‑150 (MQ=60)
                       AAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATC                                                                                                                             >  SRR19973067.2758927/1‑150 (MQ=60)
                        AGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCC                                                                                                                            >  SRR19973067.369193/1‑150 (MQ=60)
                         GTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCG                                                                                                                           >  SRR19973067.2150357/1‑150 (MQ=60)
                            CAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTC                                                                                                                        >  SRR19973067.1840755/1‑150 (MQ=60)
                             AACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCC                                                                                                                       >  SRR19973067.1338703/1‑150 (MQ=60)
                             AACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCC                                                                                                                       >  SRR19973067.1338048/1‑150 (MQ=60)
                                 GTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTG                                                                                                                   <  SRR19973067.2712430/150‑1 (MQ=60)
                                 GTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTG                                                                                                                   >  SRR19973067.2936155/1‑150 (MQ=60)
                                 GTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTG                                                                                                                   >  SRR19973067.1611510/1‑150 (MQ=60)
                                 GTTCTCTGCTGCGCGAAGAAATCGAACGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTAATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTG                                                                                                                   >  SRR19973067.2936208/1‑150 (MQ=60)
                                   TCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGC                                                                                                                 >  SRR19973067.1566086/1‑150 (MQ=60)
                                   TCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGC                                                                                                                 <  SRR19973067.317719/150‑1 (MQ=60)
                                      CTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAA                                                                                                              >  SRR19973067.1609764/1‑150 (MQ=60)
                                      CTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAA                                                                                                              >  SRR19973067.1992238/1‑150 (MQ=60)
                                      CTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAA                                                                                                              >  SRR19973067.50116/1‑150 (MQ=60)
                                        GCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAAT                                                                                                            <  SRR19973067.2612440/150‑1 (MQ=60)
                                         CTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATG                                                                                                           <  SRR19973067.3034495/150‑1 (MQ=60)
                                         CTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATG                                                                                                           <  SRR19973067.1980403/150‑1 (MQ=60)
                                              CGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGA                                                                                                      >  SRR19973067.3175749/1‑150 (MQ=60)
                                                AAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAA                                                                                                    <  SRR19973067.1931458/150‑1 (MQ=60)
                                                       CGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTT                                                                                             >  SRR19973067.2640082/1‑150 (MQ=60)
                                                       CGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTT                                                                                             <  SRR19973067.3219934/150‑1 (MQ=60)
                                                            GTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCG                                                                                        <  SRR19973067.3510386/150‑1 (MQ=60)
                                                               CCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTG                                                                                     <  SRR19973067.1234482/150‑1 (MQ=60)
                                                               CCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTG                                                                                     <  SRR19973067.1234193/150‑1 (MQ=60)
                                                                CGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGG                                                                                    >  SRR19973067.2406268/1‑150 (MQ=60)
                                                                CGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGG                                                                                    >  SRR19973067.2480755/1‑150 (MQ=60)
                                                                 GGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGC                                                                                   <  SRR19973067.794939/150‑1 (MQ=60)
                                                                 GGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGC                                                                                   <  SRR19973067.795027/150‑1 (MQ=60)
                                                                    ATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTG                                                                                >  SRR19973067.601799/1‑150 (MQ=60)
                                                                       CTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTA                                                                             <  SRR19973067.2368766/150‑1 (MQ=60)
                                                                       CTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTA                                                                             <  SRR19973067.2390523/150‑1 (MQ=60)
                                                                        TGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTAC                                                                            >  SRR19973067.1562814/1‑150 (MQ=60)
                                                                         GAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACG                                                                           <  SRR19973067.92198/150‑1 (MQ=60)
                                                                         GAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACG                                                                           <  SRR19973067.1715698/150‑1 (MQ=60)
                                                                            CAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGT                                                                        >  SRR19973067.2351620/1‑150 (MQ=60)
                                                                              AAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAG                                                                      <  SRR19973067.1919482/150‑1 (MQ=60)
                                                                              AAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAG                                                                      <  SRR19973067.3348495/150‑1 (MQ=60)
                                                                               AGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGATCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGA                                                                     >  SRR19973067.2623206/1‑150 (MQ=60)
                                                                                GACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGTGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAG                                                                    <  SRR19973067.2550873/150‑1 (MQ=60)
                                                                                    ACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTG                                                                <  SRR19973067.1934020/150‑1 (MQ=60)
                                                                                    ACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTG                                                                >  SRR19973067.1651491/1‑150 (MQ=60)
                                                                                    ACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTG                                                                <  SRR19973067.1903633/150‑1 (MQ=60)
                                                                                     CATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGC                                                               <  SRR19973067.293398/150‑1 (MQ=60)
                                                                                      ATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCG                                                              >  SRR19973067.1331942/1‑150 (MQ=60)
                                                                                         ATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTG                                                           >  SRR19973067.1652567/1‑150 (MQ=60)
                                                                                         ATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTG                                                           <  SRR19973067.2811065/150‑1 (MQ=60)
                                                                                             ATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAG                                                       >  SRR19973067.1271471/1‑150 (MQ=60)
                                                                                             ATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAG                                                       >  SRR19973067.1160803/1‑150 (MQ=60)
                                                                                             ATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAG                                                       >  SRR19973067.1160772/1‑150 (MQ=60)
                                                                                               GTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAG                                                     <  SRR19973067.432754/150‑1 (MQ=60)
                                                                                                TTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGC                                                    >  SRR19973067.1678330/1‑150 (MQ=60)
                                                                                                 TATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCG                                                   >  SRR19973067.99412/1‑150 (MQ=60)
                                                                                                     AAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTG                                               >  SRR19973067.153552/1‑150 (MQ=60)
                                                                                                         AGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTC                                           >  SRR19973067.3324617/1‑150 (MQ=60)
                                                                                                         AGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTC                                           >  SRR19973067.3319939/1‑150 (MQ=60)
                                                                                                         AGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTC                                           >  SRR19973067.3319911/1‑150 (MQ=60)
                                                                                                         AGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTC                                           >  SRR19973067.2140581/1‑150 (MQ=60)
                                                                                                            TCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGG                                        <  SRR19973067.3534663/150‑1 (MQ=60)
                                                                                                            TCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGG                                        >  SRR19973067.2237366/1‑150 (MQ=60)
                                                                                                            TCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGG                                        <  SRR19973067.702665/150‑1 (MQ=60)
                                                                                                              ATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGC                                      >  SRR19973067.3413624/1‑150 (MQ=60)
                                                                                                                CNATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGA                                    >  SRR19973067.2712734/1‑150 (MQ=60)
                                                                                                                 GATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGAT                                   >  SRR19973067.760326/1‑150 (MQ=60)
                                                                                                                   TATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCT                                 >  SRR19973067.3550449/1‑150 (MQ=60)
                                                                                                                       CGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGAT                             >  SRR19973067.2566938/1‑150 (MQ=60)
                                                                                                                       CGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGAT                             >  SRR19973067.2566865/1‑150 (MQ=60)
                                                                                                                       CGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGAT                             >  SRR19973067.2506307/1‑150 (MQ=60)
                                                                                                                       CGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGAT                             >  SRR19973067.2506278/1‑150 (MQ=60)
                                                                                                                       CGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGAT                             >  SRR19973067.2504270/1‑150 (MQ=60)
                                                                                                                              GTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGA                      >  SRR19973067.3004185/1‑150 (MQ=60)
                                                                                                                              GTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGA                      >  SRR19973067.1010937/1‑150 (MQ=60)
                                                                                                                               TAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGAT                     >  SRR19973067.2945050/1‑150 (MQ=60)
                                                                                                                               TAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGAT                     <  SRR19973067.2188411/150‑1 (MQ=60)
                                                                                                                                AAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATG                    <  SRR19973067.2003418/150‑1 (MQ=60)
                                                                                                                                   GGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCA                 <  SRR19973067.1046556/150‑1 (MQ=60)
                                                                                                                                     CGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACA               <  SRR19973067.522929/150‑1 (MQ=60)
                                                                                                                                     CGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGGTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACA               <  SRR19973067.428676/150‑1 (MQ=60)
                                                                                                                                        AGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGA            >  SRR19973067.2358546/1‑150 (MQ=60)
                                                                                                                                         GTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGAT           >  SRR19973067.2686183/1‑150 (MQ=60)
                                                                                                                                         GTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGAT           <  SRR19973067.2743055/150‑1 (MQ=60)
                                                                                                                                         GTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGAT           <  SRR19973067.952731/150‑1 (MQ=60)
                                                                                                                                         GTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGAT           <  SRR19973067.3155541/150‑1 (MQ=60)
                                                                                                                                            GATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATG        >  SRR19973067.2176752/1‑150 (MQ=60)
                                                                                                                                            GATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATG        <  SRR19973067.3039237/150‑1 (MQ=60)
                                                                                                                                            GATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATG        <  SRR19973067.166878/150‑1 (MQ=60)
                                                                                                                                            GATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATG        >  SRR19973067.3329666/1‑150 (MQ=60)
                                                                                                                                            GATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATG        >  SRR19973067.3330485/1‑150 (MQ=60)
                                                                                                                                             ATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGA       >  SRR19973067.3537653/1‑150 (MQ=60)
                                                                                                                                                 TATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAA   <  SRR19973067.3530615/150‑1 (MQ=60)
                                                                                                                                                  ATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAAC  <  SRR19973067.3019924/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
ACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAAC  >  CP009273/4172359‑4172654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑